Rh7CG149600

Sphingosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0008178
Physical Location & Seq
Forward (+)
12217468 .. 12221968
4501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG149600.1

Sequence Viewer

Length: 306 bp
ATGACGTCGTCACCGGCGGCCAAACCCTCTTGGCTCAAATCGAGCCTTCAATTTCCAGCACCAACGGGACCAAGAGGGAGGTCATCAGGCTTAAAGTGGAGGGTCTGGAGATCAGAATCGGAGCTCTGGTCGACGACGGAGGTGGAATCTGCTTTGGTAGGGAGCAGCGGAGGAGCTCTGGTCAGGAAGGACTTGGTGTTCGAGTCTTTATCAGAGGACTCCAAGAGTTTCTGCTACCAGAAGCTTCTCGAATTCATTGATTCTCTTGTGGATGGCCGAAGAGGCTTGGGTTCTTGTTTTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000287 GO:0000325 GO:0001101 GO:0001558 GO:0001727 GO:0001932 GO:0001934 GO:0001959 GO:0003376 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006066 GO:0006355 GO:0006457 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006793 GO:0006796 GO:0006807 GO:0006937 GO:0006940 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0008284 GO:0008289 GO:0008481 GO:0008610 GO:0009056 GO:0009058 GO:0009705 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010799 GO:0010800 GO:0010803 GO:0010941 GO:0016020 GO:0016042 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017050 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019722 GO:0019751 GO:0019899 GO:0019902 GO:0019903 GO:0019932 GO:0022603 GO:0023051 GO:0023052 GO:0023056 GO:0030148 GO:0030149 GO:0030258 GO:0030307 GO:0030334 GO:0030335 GO:0030554 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032870 GO:0032879 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0035556 GO:0035639 GO:0036094 GO:0038023 GO:0038036 GO:0040008 GO:0040012 GO:0040017 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044057 GO:0044093 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045125 GO:0045765 GO:0045766 GO:0045787 GO:0045927 GO:0045931 GO:0045933 GO:0045937 GO:0045987 GO:0046165 GO:0046173 GO:0046466 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0046521 GO:0046834 GO:0046872 GO:0048145 GO:0048146 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051270 GO:0051272 GO:0051716 GO:0051721 GO:0051726 GO:0060089 GO:0060255 GO:0060548 GO:0060759 GO:0065007 GO:0065009 GO:0070300 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090257 GO:0090351 GO:0090520 GO:0097159 GO:0097164 GO:0097305 GO:0097306 GO:0097367 GO:0098588 GO:0098805 GO:1901222 GO:1901224 GO:1901265 GO:1901342 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903320 GO:1903322 GO:1903506 GO:1904018 GO:2000026 GO:2000112 GO:2000145 GO:2000147 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04020 ko04071 ko04072 ko04370 ko04371 ko04666 ko05152 map00600 map01100 map04020 map04071 map04072 map04370 map04371 map04666 map05152
Pfam Domains
Protein Families
DAGK_cat

Protein Analysis

101

Amino Acids

11.11

Weight (kDa)

8.71

Isoelectric Point (pI)

65.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
AccI GTMKAC 1 cut(s) 131
AciI CCGC 2 cut(s) 17, 168
AcoI YGGCCR 2 cut(s) 18, 274
AcsI RAATTY 1 cut(s) 251
AcyI GRCGYC 1 cut(s) 5
AgsI TTSAA 1 cut(s) 50
AloI GAACNNNNNNTCC 2 cut(s) 182, 214
AluBI AGCT 3 cut(s) 124, 176, 244
AluI AGCT 3 cut(s) 124, 176, 244
Alw21I GWGCWC 2 cut(s) 126, 178
AoxI GGCC 2 cut(s) 18, 274
ApeKI GCWGC 1 cut(s) 165
ApoI RAATTY 1 cut(s) 251
ArsI GACNNNNNNTTYG 2 cut(s) 182, 214
AspS9I GGNCC 1 cut(s) 68
AsuHPI GGTGA 1 cut(s) 3
AvaII GGWCC 1 cut(s) 68
BanII GRGCYC 2 cut(s) 126, 178
Bbv12I GWGCWC 2 cut(s) 126, 178
BbvI GCAGC 1 cut(s) 177
BccI CCATC 1 cut(s) 266
BglI GCCNNNNNGGC 1 cut(s) 282
BisI GCNGC 2 cut(s) 18, 166
BlsI GCNGC 2 cut(s) 19, 167
Bme18I GGWCC 1 cut(s) 68
BmgT120I GGNCC 1 cut(s) 68
BmiI GGNNCC 1 cut(s) 69
BpmI CTGGAG 1 cut(s) 127
BsaBI GATNNNNATC 1 cut(s) 115
BsaHI GRCGYC 1 cut(s) 5
BsaXI ACNNNNNCTCC 4 cut(s) 113, 143, 165, 195
Bse118I RCCGGY 1 cut(s) 13
Bse8I GATNNNNATC 1 cut(s) 115
BseGI GGATG 1 cut(s) 277
BseJI GATNNNNATC 1 cut(s) 115
BseRI GAGGAG 1 cut(s) 186
BseXI GCAGC 1 cut(s) 177
BshFI GGCC 2 cut(s) 20, 276
BsiHKAI GWGCWC 2 cut(s) 126, 178
BsiSI CCGG 1 cut(s) 14
BslFI GGGAC 1 cut(s) 81
BsmFI GGGAC 1 cut(s) 81
BsnI GGCC 2 cut(s) 20, 276
Bsp1286I GDGCHC 2 cut(s) 126, 178
Bsp143I GATC 1 cut(s) 110
BspACI CCGC 2 cut(s) 17, 168
BspANI GGCC 2 cut(s) 20, 276
BspLI GGNNCC 1 cut(s) 69
BsrFI RCCGGY 1 cut(s) 13
BssAI RCCGGY 1 cut(s) 13
BssMI GATC 1 cut(s) 110
BssNI GRCGYC 1 cut(s) 5
Bst6I CTCTTC 1 cut(s) 274
BstACI GRCGYC 1 cut(s) 5
BstF5I GGATG 1 cut(s) 277
BstKTI GATC 1 cut(s) 113
BstMBI GATC 1 cut(s) 110
BstMWI GCNNNNNNNGC 1 cut(s) 282
BstV1I GCAGC 1 cut(s) 177
BsuRI GGCC 2 cut(s) 20, 276
BtsCI GGATG 1 cut(s) 277
Cfr10I RCCGGY 1 cut(s) 13
Cfr13I GGNCC 1 cut(s) 68
CviJI RGCY 9 cut(s) 20, 34, 45, 90, 124, 176, 244, 276, 285
CviKI_1 RGCY 9 cut(s) 20, 34, 45, 90, 124, 176, 244, 276, 285
DpnI GATC 1 cut(s) 112
DpnII GATC 1 cut(s) 110
EaeI YGGCCR 2 cut(s) 18, 274
Eam1104I CTCTTC 1 cut(s) 274
EarI CTCTTC 1 cut(s) 274
Ecl136II GAGCTC 2 cut(s) 124, 176
Eco24I GRGCYC 2 cut(s) 126, 178
Eco47I GGWCC 1 cut(s) 68
Eco53kI GAGCTC 2 cut(s) 124, 176
EcoICRI GAGCTC 2 cut(s) 124, 176
EcoRI GAATTC 1 cut(s) 251
EcoT38I GRGCYC 2 cut(s) 126, 178
FaqI GGGAC 1 cut(s) 81
FblI GTMKAC 1 cut(s) 131
Fnu4HI GCNGC 2 cut(s) 18, 166
FokI GGATG 1 cut(s) 284
FriOI GRGCYC 2 cut(s) 126, 178
Fsp4HI GCNGC 2 cut(s) 18, 166
GluI GCNGC 2 cut(s) 18, 166
GsuI CTGGAG 1 cut(s) 127
HaeIII GGCC 2 cut(s) 20, 276
HapII CCGG 1 cut(s) 14
Hin1I GRCGYC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 132
HindII GTYRAC 1 cut(s) 132
HindIII AAGCTT 1 cut(s) 242
HinfI GANTC 5 cut(s) 116, 146, 203, 218, 260
HpaII CCGG 1 cut(s) 14
HphI GGTGA 1 cut(s) 3
Hpy166II GTNNAC 1 cut(s) 132
Hpy188I TCNGA 3 cut(s) 115, 121, 214
Hpy188III TCNNGA 3 cut(s) 106, 184, 248
Hpy8I GTNNAC 1 cut(s) 132
Hpy99I CGWCG 3 cut(s) 10, 136, 139
HpyAV CCTTC 2 cut(s) 56, 181
HpyCH4IV ACGT 1 cut(s) 5
HpyF10VI GCNNNNNNNGC 1 cut(s) 282
HpySE526I ACGT 1 cut(s) 5
Hsp92I GRCGYC 1 cut(s) 5
Kzo9I GATC 1 cut(s) 110
LmnI GCTCC 3 cut(s) 121, 162, 173
LpnPI CCDG 8 cut(s) 27, 69, 72, 91, 112, 164, 169, 251
Lsp1109I GCAGC 1 cut(s) 177
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 1 cut(s) 9
MalI GATC 1 cut(s) 112
MboI GATC 1 cut(s) 110
MboII GAAGA 1 cut(s) 291
MhlI GDGCHC 2 cut(s) 126, 178
MluCI AATT 2 cut(s) 50, 251
MlyI GAGTC 2 cut(s) 212, 212
MnlI CCTC 8 cut(s) 37, 68, 72, 93, 133, 164, 208, 275
MseI TTAA 1 cut(s) 92
MspA1I CMGCKG 1 cut(s) 168
MspI CCGG 1 cut(s) 14
MwoI GCNNNNNNNGC 1 cut(s) 282
NdeII GATC 1 cut(s) 110
NlaIV GGNNCC 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 9
PfeI GAWTC 3 cut(s) 116, 146, 260
PflFI GACNNNGTC 1 cut(s) 7
PkrI GCNGC 2 cut(s) 19, 167
PleI GAGTC 2 cut(s) 211, 212
PpsI GAGTC 2 cut(s) 211, 212
Psp124BI GAGCTC 2 cut(s) 126, 178
PspN4I GGNNCC 1 cut(s) 69
PspPI GGNCC 1 cut(s) 68
PsyI GACNNNGTC 1 cut(s) 7
SacI GAGCTC 2 cut(s) 126, 178
SalI GTCGAC 1 cut(s) 130
SaqAI TTAA 1 cut(s) 92
SatI GCNGC 2 cut(s) 18, 166
Sau3AI GATC 1 cut(s) 110
Sau96I GGNCC 1 cut(s) 68
SchI GAGTC 2 cut(s) 212, 212
SduI GDGCHC 2 cut(s) 126, 178
SetI ASST 6 cut(s) 8, 83, 126, 144, 178, 246
SgrAI CRCCGGYG 1 cut(s) 13
SinI GGWCC 1 cut(s) 68
Sse9I AATT 2 cut(s) 50, 251
SsiI CCGC 2 cut(s) 17, 168
SstI GAGCTC 2 cut(s) 126, 178
TaiI ACGT 1 cut(s) 8
TaqI TCGA 4 cut(s) 41, 131, 201, 249
TasI AATT 2 cut(s) 50, 251
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 3 cut(s) 116, 146, 260
Tru1I TTAA 1 cut(s) 92
Tru9I TTAA 1 cut(s) 92
TseFI GTSAC 1 cut(s) 9
TseI GCWGC 1 cut(s) 165
Tsp45I GTSAC 1 cut(s) 9
TspDTI ATGAA 1 cut(s) 244
TspGWI ACGGA 1 cut(s) 152
Tth111I GACNNNGTC 1 cut(s) 7
VpaK11BI GGWCC 1 cut(s) 68
XapI RAATTY 1 cut(s) 251
XmiI GTMKAC 1 cut(s) 131
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.