Rh4DG247900

sphingosine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008178
Physical Location & Seq
Reverse (-)
46045804 .. 46053545
7742 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG247900.1

Sequence Viewer

Length: 501 bp
ATGAAAGAAGTGCTGTCTAGGAGTGCTCTGTTTGGTGGGACTTTTCCCGGACGAGGTGGTGGCACGGCTGCTGGTCTCATCAAGGCTGACTTGGTGACGGCGGCGGAGGCGGATATGAAGATCCAGGCTCGATGGAAATCGGAGCTCTGGTCGACGGCGGAGGTGGAATCTGCTTTGGTAGGGAGCAGCGGAGGAGCTCTGGTCAGGAAGGACTTGGTGTTCGAGCCTTTATCAGAGGACTCCAAGAGTTTCTGCTGCCAGAAGCTTCTCGAATTCATTGATTCTCTTGGACGTGGTGCCAAGGAGGCTAATTTGTACACTAAAATAGCTGAAGGCATATATTCTTATAGCATTACTCATCATCATCGTGACTCAATCACAGGATGTTGGGTGCAAAGCTGCATCATATATAGATTGTGCAGGATGGGAAAGATACAGTTCATCGGCTACCCCAAAGGAAGAGATGAGAGCATCTGCAAGAAAAATATGGACAATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000287 GO:0000325 GO:0001101 GO:0001558 GO:0001727 GO:0001932 GO:0001934 GO:0001959 GO:0003376 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004888 GO:0004930 GO:0005488 GO:0005515 GO:0005516 GO:0005524 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006066 GO:0006355 GO:0006457 GO:0006629 GO:0006643 GO:0006665 GO:0006670 GO:0006793 GO:0006796 GO:0006807 GO:0006937 GO:0006940 GO:0007154 GO:0007165 GO:0007186 GO:0007275 GO:0007346 GO:0008144 GO:0008150 GO:0008152 GO:0008284 GO:0008289 GO:0008481 GO:0008610 GO:0009056 GO:0009058 GO:0009705 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010799 GO:0010800 GO:0010803 GO:0010941 GO:0016020 GO:0016042 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017050 GO:0017076 GO:0019219 GO:0019220 GO:0019222 GO:0019722 GO:0019751 GO:0019899 GO:0019902 GO:0019903 GO:0019932 GO:0022603 GO:0023051 GO:0023052 GO:0023056 GO:0030148 GO:0030149 GO:0030258 GO:0030307 GO:0030334 GO:0030335 GO:0030554 GO:0031090 GO:0031323 GO:0031325 GO:0031326 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032870 GO:0032879 GO:0033993 GO:0034311 GO:0034312 GO:0034641 GO:0035556 GO:0035639 GO:0036094 GO:0038023 GO:0038036 GO:0040008 GO:0040012 GO:0040017 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044057 GO:0044093 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045125 GO:0045765 GO:0045766 GO:0045787 GO:0045927 GO:0045931 GO:0045933 GO:0045937 GO:0045987 GO:0046165 GO:0046173 GO:0046466 GO:0046467 GO:0046512 GO:0046519 GO:0046520 GO:0046521 GO:0046834 GO:0046872 GO:0048145 GO:0048146 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051270 GO:0051272 GO:0051716 GO:0051721 GO:0051726 GO:0060089 GO:0060255 GO:0060548 GO:0060759 GO:0065007 GO:0065009 GO:0070300 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090257 GO:0090351 GO:0090520 GO:0097159 GO:0097164 GO:0097305 GO:0097306 GO:0097367 GO:0098588 GO:0098805 GO:1901222 GO:1901224 GO:1901265 GO:1901342 GO:1901363 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1903320 GO:1903322 GO:1903506 GO:1904018 GO:2000026 GO:2000112 GO:2000145 GO:2000147 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00600 ko01100 ko04020 ko04071 ko04072 ko04370 ko04371 ko04666 ko05152 map00600 map01100 map04020 map04071 map04072 map04370 map04371 map04666 map05152
Pfam Domains
Protein Families
DAGK_cat

Protein Analysis

166

Amino Acids

18.2

Weight (kDa)

8.51

Isoelectric Point (pI)

31.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 296
AccI GTMKAC 1 cut(s) 152
AciI CCGC 5 cut(s) 101, 104, 110, 158, 189
AclWI GGATC 1 cut(s) 115
AcsI RAATTY 1 cut(s) 272
AcuI CTGAAG 1 cut(s) 351
AfaI GTAC 1 cut(s) 317
AfiI CCNNNNNNNGG 1 cut(s) 53
AjiI CACGTC 1 cut(s) 293
AjnI CCWGG 1 cut(s) 123
AloI GAACNNNNNNTCC 2 cut(s) 203, 235
AluBI AGCT 5 cut(s) 145, 197, 265, 329, 399
AluI AGCT 5 cut(s) 145, 197, 265, 329, 399
Alw21I GWGCWC 3 cut(s) 28, 147, 199
Alw26I GTCTC 1 cut(s) 80
AlwI GGATC 1 cut(s) 115
ApeKI GCWGC 4 cut(s) 68, 186, 255, 399
ApoI RAATTY 1 cut(s) 272
ArsI GACNNNNNNTTYG 2 cut(s) 203, 235
AsuC2I CCSGG 1 cut(s) 48
AsuHPI GGTGA 1 cut(s) 106
BanI GGYRCC 1 cut(s) 296
BanII GRGCYC 2 cut(s) 147, 199
Bbv12I GWGCWC 3 cut(s) 28, 147, 199
BbvI GCAGC 4 cut(s) 55, 198, 242, 386
BccI CCATC 2 cut(s) 126, 418
BceAI ACGGC 3 cut(s) 81, 114, 171
BciT130I CCWGG 1 cut(s) 125
BcnI CCSGG 1 cut(s) 48
BcoDI GTCTC 1 cut(s) 80
BfaI CTAG 1 cut(s) 18
BglI GCCNNNNNGGC 1 cut(s) 305
BisI GCNGC 5 cut(s) 69, 102, 187, 256, 400
BlsI GCNGC 5 cut(s) 70, 103, 188, 257, 401
Bme1390I CCNGG 2 cut(s) 48, 125
BmgBI CACGTC 1 cut(s) 293
BmiI GGNNCC 1 cut(s) 298
BmrFI CCNGG 2 cut(s) 48, 125
BmsI GCATC 2 cut(s) 411, 480
BpuMI CCSGG 1 cut(s) 48
BsaBI GATNNNNATC 1 cut(s) 136
BsaI GGTCTC 1 cut(s) 80
BsaJI CCNNGG 1 cut(s) 300
BsaXI ACNNNNNCTCC 4 cut(s) 134, 164, 186, 216
Bsc4I CCNNNNNNNGG 1 cut(s) 53
Bse8I GATNNNNATC 1 cut(s) 136
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 1 cut(s) 300
BseGI GGATG 2 cut(s) 389, 429
BseJI GATNNNNATC 1 cut(s) 136
BseLI CCNNNNNNNGG 1 cut(s) 53
BseRI GAGGAG 1 cut(s) 207
BseXI GCAGC 4 cut(s) 55, 198, 242, 386
BsgI GTGCAG 1 cut(s) 439
BshNI GGYRCC 1 cut(s) 296
BsiHKAI GWGCWC 3 cut(s) 28, 147, 199
BsiSI CCGG 1 cut(s) 48
BslFI GGGAC 1 cut(s) 52
BslI CCNNNNNNNGG 1 cut(s) 53
BsmAI GTCTC 1 cut(s) 80
BsmFI GGGAC 1 cut(s) 52
Bso31I GGTCTC 1 cut(s) 80
Bsp1286I GDGCHC 3 cut(s) 28, 147, 199
Bsp1407I TGTACA 1 cut(s) 315
Bsp143I GATC 1 cut(s) 120
BspACI CCGC 5 cut(s) 101, 104, 110, 158, 189
BspLI GGNNCC 1 cut(s) 298
BspPI GGATC 1 cut(s) 115
BspT107I GGYRCC 1 cut(s) 296
BspTNI GGTCTC 1 cut(s) 80
BsrGI TGTACA 1 cut(s) 315
BssECI CCNNGG 1 cut(s) 300
BssMI GATC 1 cut(s) 120
BssT1I CCWWGG 1 cut(s) 300
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 1 cut(s) 438
Bst6I CTCTTC 1 cut(s) 454
BstAUI TGTACA 1 cut(s) 315
BstF5I GGATG 2 cut(s) 389, 429
BstKTI GATC 1 cut(s) 123
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 1 cut(s) 120
BstMWI GCNNNNNNNGC 2 cut(s) 107, 305
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 2 cut(s) 46, 123
BstV1I GCAGC 4 cut(s) 55, 198, 242, 386
BstX2I RGATCY 1 cut(s) 120
BstYI RGATCY 1 cut(s) 120
BtrI CACGTC 1 cut(s) 293
BtsCI GGATG 2 cut(s) 389, 429
Csp6I GTAC 1 cut(s) 316
CviQI GTAC 1 cut(s) 316
DpnI GATC 1 cut(s) 122
DpnII GATC 1 cut(s) 120
Eam1104I CTCTTC 1 cut(s) 454
EarI CTCTTC 1 cut(s) 454
EciI GGCGGA 3 cut(s) 119, 125, 173
Ecl136II GAGCTC 2 cut(s) 145, 197
Eco130I CCWWGG 1 cut(s) 300
Eco24I GRGCYC 2 cut(s) 147, 199
Eco31I GGTCTC 1 cut(s) 80
Eco53kI GAGCTC 2 cut(s) 145, 197
Eco57I CTGAAG 1 cut(s) 351
EcoICRI GAGCTC 2 cut(s) 145, 197
EcoRI GAATTC 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 123
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 2 cut(s) 147, 199
ErhI CCWWGG 1 cut(s) 300
FaiI YATR 8 cut(s) 116, 338, 340, 348, 407, 409, 411, 488
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FaqI GGGAC 1 cut(s) 52
FblI GTMKAC 1 cut(s) 152
Fnu4HI GCNGC 5 cut(s) 69, 102, 187, 256, 400
FokI GGATG 2 cut(s) 396, 436
FriOI GRGCYC 2 cut(s) 147, 199
Fsp4HI GCNGC 5 cut(s) 69, 102, 187, 256, 400
FspBI CTAG 1 cut(s) 18
GluI GCNGC 5 cut(s) 69, 102, 187, 256, 400
HapII CCGG 1 cut(s) 48
HincII GTYRAC 1 cut(s) 153
HindII GTYRAC 1 cut(s) 153
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 4 cut(s) 167, 239, 281, 371
HpaII CCGG 1 cut(s) 48
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 2 cut(s) 153, 318
Hpy188I TCNGA 2 cut(s) 142, 235
Hpy188III TCNNGA 3 cut(s) 205, 269, 368
Hpy8I GTNNAC 2 cut(s) 153, 318
Hpy99I CGWCG 1 cut(s) 157
HpyAV CCTTC 2 cut(s) 202, 326
HpyCH4III ACNGT 1 cut(s) 438
HpyCH4IV ACGT 1 cut(s) 292
HpyCH4V TGCA 4 cut(s) 394, 402, 420, 477
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 305
HpySE526I ACGT 1 cut(s) 292
Kzo9I GATC 1 cut(s) 120
LmnI GCTCC 3 cut(s) 142, 183, 194
Lsp1109I GCAGC 4 cut(s) 55, 198, 242, 386
LweI GCATC 2 cut(s) 411, 480
MaeI CTAG 1 cut(s) 18
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 2 cut(s) 94, 368
MalI GATC 1 cut(s) 122
MboI GATC 1 cut(s) 120
MboII GAAGA 2 cut(s) 130, 471
MflI RGATCY 1 cut(s) 120
MhlI GDGCHC 3 cut(s) 28, 147, 199
MluCI AATT 2 cut(s) 272, 310
MlyI GAGTC 2 cut(s) 233, 365
MnlI CCTC 6 cut(s) 47, 100, 154, 185, 229, 298
MslI CAYNNNNRTG 1 cut(s) 366
MspA1I CMGCKG 1 cut(s) 189
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 2 cut(s) 48, 125
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 2 cut(s) 107, 305
NciI CCSGG 1 cut(s) 48
NdeII GATC 1 cut(s) 120
NlaIV GGNNCC 1 cut(s) 298
NmuCI GTSAC 2 cut(s) 94, 368
PfeI GAWTC 2 cut(s) 167, 281
PfoI TCCNGGA 1 cut(s) 46
PkrI GCNGC 5 cut(s) 70, 103, 188, 257, 401
PleI GAGTC 2 cut(s) 233, 365
PpsI GAGTC 2 cut(s) 233, 365
Psp124BI GAGCTC 2 cut(s) 147, 199
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 1 cut(s) 298
PsuI RGATCY 1 cut(s) 120
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
RseI CAYNNNNRTG 1 cut(s) 366
SacI GAGCTC 2 cut(s) 147, 199
SalI GTCGAC 1 cut(s) 151
SatI GCNGC 5 cut(s) 69, 102, 187, 256, 400
Sau3AI GATC 1 cut(s) 120
SchI GAGTC 2 cut(s) 233, 365
ScrFI CCNGG 2 cut(s) 48, 125
SduI GDGCHC 3 cut(s) 28, 147, 199
SetI ASST 8 cut(s) 58, 147, 165, 199, 267, 295, 331, 401
SfaNI GCATC 2 cut(s) 411, 480
SmiMI CAYNNNNRTG 1 cut(s) 366
Sse9I AATT 2 cut(s) 272, 310
SsiI CCGC 5 cut(s) 101, 104, 110, 158, 189
SspI AATATT 1 cut(s) 496
SspMI CTAG 1 cut(s) 18
SstI GAGCTC 2 cut(s) 147, 199
StyD4I CCNGG 2 cut(s) 46, 123
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 1 cut(s) 438
TaiI ACGT 1 cut(s) 295
TaqI TCGA 4 cut(s) 130, 152, 222, 270
TasI AATT 2 cut(s) 272, 310
TatI WGTACW 1 cut(s) 315
TauI GCSGC 1 cut(s) 104
TfiI GAWTC 2 cut(s) 167, 281
TseFI GTSAC 2 cut(s) 94, 368
TseI GCWGC 4 cut(s) 68, 186, 255, 399
Tsp45I GTSAC 2 cut(s) 94, 368
TspDTI ATGAA 4 cut(s) 17, 131, 265, 430
XapI RAATTY 1 cut(s) 272
XmiI GTMKAC 1 cut(s) 152
XspI CTAG 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.