Rh5BG314700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0006316
Physical Location & Seq
Forward (+)
41740285 .. 41768477
28193 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG314700.1

Sequence Viewer

Length: 477 bp
ATGGCTTCGAATACGAATCAGTCTCTGCTGCCGGAGATCGGTCTCGACGGCCTTACTCGGGAGGCCTCCGTCGTTAACTACACTAAGAAAGTGATCGAGGAAGAACAACATCAATTGAGGAAATATATAGAAGAAAATTACTCTAAAATCCACGATGTTGATGGTGGGAATTGCTTCTCGGGTCAACCCAAACACGAAACGGCCAATCTATTGCAGTCGTTTATTGACAACCTTGACAACGTTTTGCCGCAGGGGATTTTGGACGACAAGAGAGGAGCACTGACGGTGCCAACGTCGAGCGGAGAAGTGAAGCCTTTGACTGCCGAGGAATTGGAGCAGTACGAGTCCGTTTCAGAGTCCTCCAACTCTGACTCCGACGATGGCCTCGAAGACGAGGAAGAGGACGGCGACGAAGACGAGGATGACGAGGACGATGAGGATGAAGATGACGACGACGATGTCCAGGAGGTCGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transcrip_act

Protein Analysis

158

Amino Acids

17.58

Weight (kDa)

4.05

Isoelectric Point (pI)

60.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 458
AccB1I GGYRCC 1 cut(s) 286
AccBSI CCGCTC 1 cut(s) 300
AciI CCGC 2 cut(s) 248, 300
AclI AACGTT 1 cut(s) 240
AcoI YGGCCR 1 cut(s) 201
AfaI GTAC 1 cut(s) 341
AfiI CCNNNNNNNGG 2 cut(s) 38, 58
AjnI CCWGG 1 cut(s) 462
Alw21I GWGCWC 1 cut(s) 280
Alw26I GTCTC 2 cut(s) 27, 47
AlwNI CAGNNNCTG 1 cut(s) 25
Ama87I CYCGRG 2 cut(s) 57, 178
AoxI GGCC 4 cut(s) 49, 63, 201, 382
ApeKI GCWGC 1 cut(s) 28
Asp700I GAANNNNTTC 1 cut(s) 173
AsuII TTCGAA 1 cut(s) 8
AvaI CYCGRG 2 cut(s) 57, 178
BanI GGYRCC 1 cut(s) 286
BbsI GAAGAC 2 cut(s) 396, 420
Bbv12I GWGCWC 1 cut(s) 280
BbvI GCAGC 1 cut(s) 15
BccI CCATC 2 cut(s) 155, 374
BceAI ACGGC 3 cut(s) 64, 216, 421
BciT130I CCWGG 1 cut(s) 464
BcoDI GTCTC 2 cut(s) 27, 47
BisI GCNGC 2 cut(s) 29, 248
BlsI GCNGC 2 cut(s) 30, 249
Bme1390I CCNGG 1 cut(s) 464
BmeT110I CYCGRG 2 cut(s) 57, 178
BmiI GGNNCC 1 cut(s) 288
BmrFI CCNGG 1 cut(s) 464
BpiI GAAGAC 2 cut(s) 396, 420
Bpu14I TTCGAA 1 cut(s) 8
BsaI GGTCTC 1 cut(s) 47
BsaJI CCNNGG 1 cut(s) 324
BsaXI ACNNNNNCTCC 2 cut(s) 356, 386
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 58
BseBI CCWGG 1 cut(s) 464
BseDI CCNNGG 1 cut(s) 324
BseGI GGATG 2 cut(s) 427, 445
BseLI CCNNNNNNNGG 2 cut(s) 38, 58
BseRI GAGGAG 1 cut(s) 288
BseXI GCAGC 1 cut(s) 15
BshFI GGCC 4 cut(s) 51, 65, 203, 384
BshNI GGYRCC 1 cut(s) 286
BsiHKAI GWGCWC 1 cut(s) 280
BsiHKCI CYCGRG 2 cut(s) 57, 178
BsiSI CCGG 1 cut(s) 32
BslI CCNNNNNNNGG 2 cut(s) 38, 58
BsmAI GTCTC 2 cut(s) 27, 47
BsnI GGCC 4 cut(s) 51, 65, 203, 384
Bso31I GGTCTC 1 cut(s) 47
BsoBI CYCGRG 2 cut(s) 57, 178
Bsp119I TTCGAA 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 280
Bsp143I GATC 2 cut(s) 36, 93
BspACI CCGC 2 cut(s) 248, 300
BspANI GGCC 4 cut(s) 51, 65, 203, 384
BspLI GGNNCC 1 cut(s) 288
BspT104I TTCGAA 1 cut(s) 8
BspT107I GGYRCC 1 cut(s) 286
BspTNI GGTCTC 1 cut(s) 47
BsrBI CCGCTC 1 cut(s) 300
BssECI CCNNGG 1 cut(s) 324
BssMI GATC 2 cut(s) 36, 93
Bst2UI CCWGG 1 cut(s) 464
Bst4CI ACNGT 1 cut(s) 286
Bst6I CTCTTC 1 cut(s) 393
BstBI TTCGAA 1 cut(s) 8
BstDEI CTNAG 1 cut(s) 84
BstF5I GGATG 2 cut(s) 427, 445
BstKTI GATC 2 cut(s) 39, 96
BstMAI GTCTC 2 cut(s) 27, 47
BstMBI GATC 2 cut(s) 36, 93
BstNI CCWGG 1 cut(s) 464
BstSCI CCNGG 1 cut(s) 462
BstV1I GCAGC 1 cut(s) 15
BstV2I GAAGAC 2 cut(s) 396, 420
BsuRI GGCC 4 cut(s) 51, 65, 203, 384
BtsCI GGATG 2 cut(s) 427, 445
BtsIMutI CAGTG 1 cut(s) 278
CaiI CAGNNNCTG 1 cut(s) 25
Csp6I GTAC 1 cut(s) 340
CviJI RGCY 6 cut(s) 5, 51, 65, 203, 313, 384
CviKI_1 RGCY 6 cut(s) 5, 51, 65, 203, 313, 384
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 1 cut(s) 84
DpnI GATC 2 cut(s) 38, 95
DpnII GATC 2 cut(s) 36, 93
DrdI GACNNNNNNGTC 1 cut(s) 458
DseDI GACNNNNNNGTC 1 cut(s) 458
EaeI YGGCCR 1 cut(s) 201
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
Eco147I AGGCCT 1 cut(s) 65
Eco31I GGTCTC 1 cut(s) 47
Eco88I CYCGRG 2 cut(s) 57, 178
EcoRII CCWGG 1 cut(s) 462
FaiI YATR 3 cut(s) 126, 128, 475
Fnu4HI GCNGC 2 cut(s) 29, 248
FokI GGATG 2 cut(s) 434, 452
Fsp4HI GCNGC 2 cut(s) 29, 248
GluI GCNGC 2 cut(s) 29, 248
HaeIII GGCC 4 cut(s) 51, 65, 203, 384
HapII CCGG 1 cut(s) 32
HincII GTYRAC 2 cut(s) 76, 185
HindII GTYRAC 2 cut(s) 76, 185
HinfI GANTC 4 cut(s) 16, 344, 356, 371
HpaI GTTAAC 1 cut(s) 76
HpaII CCGG 1 cut(s) 32
Hpy166II GTNNAC 2 cut(s) 76, 185
Hpy188I TCNGA 3 cut(s) 355, 370, 376
Hpy188III TCNNGA 2 cut(s) 44, 59
Hpy8I GTNNAC 2 cut(s) 76, 185
Hpy99I CGWCG 7 cut(s) 50, 74, 298, 380, 413, 455, 458
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 2 cut(s) 240, 293
HpyCH4V TGCA 1 cut(s) 214
HpyF3I CTNAG 1 cut(s) 84
HpySE526I ACGT 2 cut(s) 240, 293
KspAI GTTAAC 1 cut(s) 76
Kzo9I GATC 2 cut(s) 36, 93
LmnI GCTCC 2 cut(s) 275, 334
LpnPI CCDG 3 cut(s) 45, 236, 449
Lsp1109I GCAGC 1 cut(s) 15
MaeII ACGT 2 cut(s) 240, 293
MalI GATC 2 cut(s) 38, 95
MbiI CCGCTC 1 cut(s) 300
MboI GATC 2 cut(s) 36, 93
MboII GAAGA 6 cut(s) 113, 143, 401, 410, 425, 455
MfeI CAATTG 1 cut(s) 113
MhlI GDGCHC 1 cut(s) 280
MluCI AATT 4 cut(s) 113, 136, 169, 329
MlyI GAGTC 3 cut(s) 353, 365, 365
MmeI TCCRAC 2 cut(s) 387, 399
MroXI GAANNNNTTC 1 cut(s) 173
MseI TTAA 1 cut(s) 75
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 1 cut(s) 464
MunI CAATTG 1 cut(s) 113
MvaI CCWGG 1 cut(s) 464
NdeII GATC 2 cut(s) 36, 93
NlaIV GGNNCC 1 cut(s) 288
NmeAIII GCCGAG 1 cut(s) 349
NspV TTCGAA 1 cut(s) 8
PceI AGGCCT 1 cut(s) 65
PcsI WCGNNNNNNNCGW 3 cut(s) 290, 384, 423
PdmI GAANNNNTTC 1 cut(s) 173
PfeI GAWTC 1 cut(s) 16
PflFI GACNNNGTC 1 cut(s) 458
PfoI TCCNGGA 1 cut(s) 462
PkrI GCNGC 2 cut(s) 30, 249
PleI GAGTC 3 cut(s) 352, 364, 365
PpsI GAGTC 3 cut(s) 352, 364, 365
Psp1406I AACGTT 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 462
PspGI CCWGG 1 cut(s) 462
PspN4I GGNNCC 1 cut(s) 288
PstNI CAGNNNCTG 1 cut(s) 25
PsyI GACNNNGTC 1 cut(s) 458
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 2 cut(s) 29, 248
Sau3AI GATC 2 cut(s) 36, 93
SchI GAGTC 3 cut(s) 353, 365, 365
ScrFI CCNGG 1 cut(s) 464
SduI GDGCHC 1 cut(s) 280
SetI ASST 4 cut(s) 234, 243, 296, 471
SfuI TTCGAA 1 cut(s) 8
Sse9I AATT 4 cut(s) 113, 136, 169, 329
SseBI AGGCCT 1 cut(s) 65
SsiI CCGC 2 cut(s) 248, 300
StuI AGGCCT 1 cut(s) 65
StyD4I CCNGG 1 cut(s) 462
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 2 cut(s) 243, 296
TaqI TCGA 5 cut(s) 8, 45, 96, 296, 387
TaqII GACCGA 1 cut(s) 29
TasI AATT 4 cut(s) 113, 136, 169, 329
TauI GCSGC 1 cut(s) 250
TfiI GAWTC 1 cut(s) 16
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TscAI CASTG 1 cut(s) 285
TseI GCWGC 1 cut(s) 28
TspDTI ATGAA 1 cut(s) 456
TspGWI ACGGA 2 cut(s) 58, 337
TspRI CASTG 1 cut(s) 285
Tth111I GACNNNGTC 1 cut(s) 458
XcmI CCANNNNNNNNNTGG 1 cut(s) 158
XmnI GAANNNNTTC 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.