Rh6AG386100

Belongs to the ATPase B chain family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0007596
Physical Location & Seq
Reverse (-)
58574760 .. 58575392
633 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG386100.1

Sequence Viewer

Length: 633 bp
ATGGCCAACATGATAATGGCCTCCTCCAAGGTCCCAATCTCCTCGGCGGCCCCTTCCCCCATTGCCAAGCCCAAGCTCGTCCCCGTCCAAATCTCCATCCCCAAGCTCACCAAACCCCACCTCCTCCAATCCCTCAAATCCCTCTCCCTAATCGCCGCCACCTCCCTCACCGTCGCGCCGCCGTCCCTCGCCGCCGAGATCGAGAAGGCGGCGCTCTTCGACTTCGACCTGACCCTGCCGATCCAGGCCGTCCAGTTCCTCCTCCTCATGGTGGCCCTCGACAAGATCTACTACTCGCCGCTCGGTAAGTTCATGGACGAGCGCGACGCCGACATCAGGGAGAAGCTGGGGAGCGTGAAGGACACGTCGGCGGAGGTGAAGCAGCTGGAGGAGCAGGGCGTGGCGATCATGAGGGCGGCGAGGGCGGAGATATCGGCGGCGCTGTCCAAGATGAAGAAGGAGACCCAGGCGGAGGTGGAGGTGAAGCTGGCGGAGGGGAGGAAGAAGGTGGAGGCAGAGTTGCAGGAGGCCTTGGGGAAATTGGAGGAGCAGAAGGAGGAGACTTTGAAGTCCTTGGACGCCCAGATTGCTAATCTCAGTGACCAAATTGTCAAGAAGGTTCTTCCTCTGTGA

Protein Analysis

210

Amino Acids

22.81

Weight (kDa)

6.65

Isoelectric Point (pI)

37.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATP-synt_B PF00430 78 - 208 2.5e-13 ATP synthase B/B' CF(0)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 568, 608
AccBSI CCGCTC 1 cut(s) 301
AccII CGCG 2 cut(s) 176, 324
AclWI GGATC 1 cut(s) 235
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 2 cut(s) 327, 579
AfiI CCNNNNNNNGG 4 cut(s) 268, 271, 336, 472
AflIII ACRYGT 1 cut(s) 363
AgsI TTSAA 1 cut(s) 568
AjiI CACGTC 1 cut(s) 366
AjnI CCWGG 2 cut(s) 243, 465
AluBI AGCT 5 cut(s) 76, 106, 346, 385, 487
AluI AGCT 5 cut(s) 76, 106, 346, 385, 487
Alw26I GTCTC 2 cut(s) 455, 554
AlwI GGATC 1 cut(s) 235
AoxI GGCC 6 cut(s) 3, 18, 48, 246, 273, 528
ApeKI GCWGC 1 cut(s) 382
AspLEI GCGC 4 cut(s) 178, 214, 324, 442
AspS9I GGNCC 3 cut(s) 31, 49, 274
AsuHPI GGTGA 4 cut(s) 100, 160, 388, 493
AvaII GGWCC 1 cut(s) 31
BalI TGGCCA 1 cut(s) 5
BbvI GCAGC 1 cut(s) 394
BccI CCATC 1 cut(s) 104
BceAI ACGGC 2 cut(s) 166, 233
BciT130I CCWGG 2 cut(s) 245, 467
BcoDI GTCTC 2 cut(s) 455, 554
BfoI RGCGCY 2 cut(s) 215, 443
BglII AGATCT 1 cut(s) 285
BisI GCNGC 9 cut(s) 48, 156, 179, 192, 210, 299, 383, 417, 438
BlsI GCNGC 9 cut(s) 49, 157, 180, 193, 211, 300, 384, 418, 439
Bme1390I CCNGG 2 cut(s) 245, 467
Bme18I GGWCC 1 cut(s) 31
BmgBI CACGTC 1 cut(s) 366
BmgT120I GGNCC 3 cut(s) 31, 49, 274
BmiI GGNNCC 2 cut(s) 33, 51
BmrFI CCNGG 2 cut(s) 245, 467
BpmI CTGGAG 1 cut(s) 407
BsaHI GRCGYC 2 cut(s) 327, 579
BsaI GGTCTC 1 cut(s) 455
BsaJI CCNNGG 5 cut(s) 27, 42, 465, 531, 573
BsaXI ACNNNNNCTCC 4 cut(s) 105, 135, 503, 533
Bsc4I CCNNNNNNNGG 4 cut(s) 268, 271, 336, 472
Bse1I ACTGG 1 cut(s) 253
Bse3DI GCAATG 1 cut(s) 60
BseBI CCWGG 2 cut(s) 245, 467
BseDI CCNNGG 5 cut(s) 27, 42, 465, 531, 573
BseGI GGATG 1 cut(s) 96
BseLI CCNNNNNNNGG 4 cut(s) 268, 271, 336, 472
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 610
BseNI ACTGG 1 cut(s) 253
BseRI GAGGAG 8 cut(s) 13, 31, 113, 251, 254, 404, 560, 572
BseXI GCAGC 1 cut(s) 394
BseYI CCCAGC 1 cut(s) 346
Bsh1236I CGCG 2 cut(s) 176, 324
BshFI GGCC 6 cut(s) 5, 20, 50, 248, 275, 530
BslFI GGGAC 3 cut(s) 17, 65, 169
BslI CCNNNNNNNGG 4 cut(s) 268, 271, 336, 472
BsmAI GTCTC 2 cut(s) 455, 554
BsmFI GGGAC 3 cut(s) 17, 65, 169
BsnI GGCC 6 cut(s) 5, 20, 50, 248, 275, 530
Bso31I GGTCTC 1 cut(s) 455
Bsp143I GATC 4 cut(s) 198, 240, 285, 405
BspANI GGCC 6 cut(s) 5, 20, 50, 248, 275, 530
BspCNI CTCAG 1 cut(s) 609
BspFNI CGCG 2 cut(s) 176, 324
BspHI TCATGA 1 cut(s) 408
BspLI GGNNCC 2 cut(s) 33, 51
BspPI GGATC 1 cut(s) 235
BspQI GCTCTTC 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 455
BsrBI CCGCTC 1 cut(s) 301
BsrDI GCAATG 1 cut(s) 60
BsrI ACTGG 1 cut(s) 253
BssECI CCNNGG 5 cut(s) 27, 42, 465, 531, 573
BssMI GATC 4 cut(s) 198, 240, 285, 405
BssNI GRCGYC 2 cut(s) 327, 579
BssT1I CCWWGG 3 cut(s) 27, 531, 573
Bst2UI CCWGG 2 cut(s) 245, 467
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 1 cut(s) 221
BstACI GRCGYC 2 cut(s) 327, 579
BstC8I GCNNGC 1 cut(s) 489
BstDEI CTNAG 1 cut(s) 596
BstF5I GGATG 1 cut(s) 96
BstFNI CGCG 2 cut(s) 176, 324
BstH2I RGCGCY 2 cut(s) 215, 443
BstHHI GCGC 4 cut(s) 178, 214, 324, 442
BstKTI GATC 4 cut(s) 201, 243, 288, 408
BstMAI GTCTC 2 cut(s) 455, 554
BstMBI GATC 4 cut(s) 198, 240, 285, 405
BstMWI GCNNNNNNNGC 3 cut(s) 391, 422, 587
BstNI CCWGG 2 cut(s) 245, 467
BstSCI CCNGG 2 cut(s) 243, 465
BstUI CGCG 2 cut(s) 176, 324
BstV1I GCAGC 1 cut(s) 394
BstX2I RGATCY 1 cut(s) 285
BstYI RGATCY 1 cut(s) 285
BsuRI GGCC 6 cut(s) 5, 20, 50, 248, 275, 530
BtrI CACGTC 1 cut(s) 366
BtsCI GGATG 1 cut(s) 96
BtsIMutI CAGTG 1 cut(s) 604
Cac8I GCNNGC 1 cut(s) 489
CciI TCATGA 1 cut(s) 408
CfoI GCGC 4 cut(s) 178, 214, 324, 442
Cfr13I GGNCC 3 cut(s) 31, 49, 274
CseI GACGC 2 cut(s) 335, 587
CviAII CATG 4 cut(s) 10, 268, 313, 409
DdeI CTNAG 1 cut(s) 596
DpnI GATC 4 cut(s) 200, 242, 287, 407
DpnII GATC 4 cut(s) 198, 240, 285, 405
DrdI GACNNNNNNGTC 2 cut(s) 568, 608
DseDI GACNNNNNNGTC 2 cut(s) 568, 608
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
EciI GGCGGA 4 cut(s) 386, 440, 485, 506
Eco130I CCWWGG 3 cut(s) 27, 531, 573
Eco147I AGGCCT 1 cut(s) 530
Eco31I GGTCTC 1 cut(s) 455
Eco32I GATATC 1 cut(s) 432
Eco47I GGWCC 1 cut(s) 31
EcoO109I RGGNCCY 1 cut(s) 31
EcoRII CCWGG 2 cut(s) 243, 465
EcoRV GATATC 1 cut(s) 432
EcoT14I CCWWGG 3 cut(s) 27, 531, 573
ErhI CCWWGG 3 cut(s) 27, 531, 573
FaeI CATG 4 cut(s) 13, 271, 316, 412
FaiI YATR 4 cut(s) 11, 269, 314, 410
FaqI GGGAC 3 cut(s) 17, 65, 169
FatI CATG 4 cut(s) 9, 267, 312, 408
Fnu4HI GCNGC 9 cut(s) 48, 156, 179, 192, 210, 299, 383, 417, 438
FokI GGATG 1 cut(s) 83
Fsp4HI GCNGC 9 cut(s) 48, 156, 179, 192, 210, 299, 383, 417, 438
GlaI GCGC 4 cut(s) 177, 213, 323, 441
GluI GCNGC 9 cut(s) 48, 156, 179, 192, 210, 299, 383, 417, 438
GsaI CCCAGC 1 cut(s) 350
GsuI CTGGAG 1 cut(s) 407
HaeII RGCGCY 2 cut(s) 215, 443
HaeIII GGCC 6 cut(s) 5, 20, 50, 248, 275, 530
HgaI GACGC 2 cut(s) 335, 587
HhaI GCGC 4 cut(s) 178, 214, 324, 442
Hin1I GRCGYC 2 cut(s) 327, 579
Hin1II CATG 4 cut(s) 13, 271, 316, 412
Hin6I GCGC 4 cut(s) 176, 212, 322, 440
HinP1I GCGC 4 cut(s) 176, 212, 322, 440
HphI GGTGA 4 cut(s) 100, 160, 388, 493
Hpy188III TCNNGA 3 cut(s) 202, 409, 613
Hpy99I CGWCG 3 cut(s) 176, 329, 370
HpyAV CCTTC 7 cut(s) 63, 199, 352, 451, 499, 547, 610
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 1 cut(s) 365
HpyCH4V TGCA 1 cut(s) 523
HpyF10VI GCNNNNNNNGC 3 cut(s) 391, 422, 587
HpyF3I CTNAG 1 cut(s) 596
HpySE526I ACGT 1 cut(s) 365
Hsp92I GRCGYC 2 cut(s) 327, 579
Hsp92II CATG 4 cut(s) 13, 271, 316, 412
HspAI GCGC 4 cut(s) 176, 212, 322, 440
Kzo9I GATC 4 cut(s) 198, 240, 285, 405
LguI GCTCTTC 1 cut(s) 221
LmnI GCTCC 3 cut(s) 351, 391, 547
Lsp1109I GCAGC 1 cut(s) 394
MaeII ACGT 1 cut(s) 365
MaeIII GTNAC 1 cut(s) 599
MalI GATC 4 cut(s) 200, 242, 287, 407
MbiI CCGCTC 1 cut(s) 301
MboI GATC 4 cut(s) 198, 240, 285, 405
MboII GAAGA 4 cut(s) 208, 466, 514, 614
MflI RGATCY 1 cut(s) 285
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 539, 606
MluNI TGGCCA 1 cut(s) 5
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 14
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 385
MspR9I CCNGG 2 cut(s) 245, 467
MvaI CCWGG 2 cut(s) 245, 467
MvnI CGCG 2 cut(s) 176, 324
MwoI GCNNNNNNNGC 3 cut(s) 391, 422, 587
NdeII GATC 4 cut(s) 198, 240, 285, 405
NlaIII CATG 4 cut(s) 13, 271, 316, 412
NlaIV GGNNCC 2 cut(s) 33, 51
NmeAIII GCCGAG 2 cut(s) 23, 220
NmuCI GTSAC 1 cut(s) 599
PagI TCATGA 1 cut(s) 408
PceI AGGCCT 1 cut(s) 530
PciSI GCTCTTC 1 cut(s) 221
PkrI GCNGC 9 cut(s) 49, 157, 180, 193, 211, 300, 384, 418, 439
PpuMI RGGWCCY 1 cut(s) 31
Psp5II RGGWCCY 1 cut(s) 31
Psp6I CCWGG 2 cut(s) 243, 465
PspFI CCCAGC 1 cut(s) 346
PspGI CCWGG 2 cut(s) 243, 465
PspN4I GGNNCC 2 cut(s) 33, 51
PspPI GGNCC 3 cut(s) 31, 49, 274
PspPPI RGGWCCY 1 cut(s) 31
PsuI RGATCY 1 cut(s) 285
PvuII CAGCTG 1 cut(s) 385
RseI CAYNNNNRTG 1 cut(s) 14
SapI GCTCTTC 1 cut(s) 221
SatI GCNGC 9 cut(s) 48, 156, 179, 192, 210, 299, 383, 417, 438
Sau3AI GATC 4 cut(s) 198, 240, 285, 405
Sau96I GGNCC 3 cut(s) 31, 49, 274
ScrFI CCNGG 2 cut(s) 245, 467
SinI GGWCC 1 cut(s) 31
SmiMI CAYNNNNRTG 1 cut(s) 14
Sse9I AATT 2 cut(s) 539, 606
SseBI AGGCCT 1 cut(s) 530
StuI AGGCCT 1 cut(s) 530
StyD4I CCNGG 2 cut(s) 243, 465
StyI CCWWGG 3 cut(s) 27, 531, 573
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 1 cut(s) 368
TaqI TCGA 4 cut(s) 201, 219, 225, 279
TasI AATT 2 cut(s) 539, 606
TauI GCSGC 8 cut(s) 50, 158, 181, 194, 212, 301, 419, 440
TscAI CASTG 1 cut(s) 604
TseFI GTSAC 1 cut(s) 599
TseI GCWGC 1 cut(s) 382
Tsp45I GTSAC 1 cut(s) 599
TspDTI ATGAA 2 cut(s) 301, 467
TspRI CASTG 1 cut(s) 604
VpaK11BI GGWCC 1 cut(s) 31
XcmI CCANNNNNNNNNTGG 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.