Rh6BG050600

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001747
Physical Location & Seq
Reverse (-)
8201446 .. 8205326
3881 bp
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UTR
Exon/CDS
Intron
Rh6BG050600.1

Sequence Viewer

Length: 576 bp
ATGCTTCTTTGGGAATGTGTTTGCCTTCTGGATGCTTCTTATACCAGGTTCATATGTAAGAGATATATAGGAGAGGAGGTGGACTCGGAAGACAATATTAGGAAGGTTGTGTCAGCCGGACCAGAAGACAGCTTTCCACCCTCATTCAAGCACTTGGTTATTTTGGATATGGCTGAGAGAGAACAGCAGCACCAGGGTGATGCACAGATGCAATCTCCAAAGGATGACATGGTGGCATGTGTGATGGCATTGGAGGCTGCTTTGCTTCCATGCTTGCCTGCCAGAGAGCTTCAAGCAATAGACCGTTCACCGCACCCGTCTCATCAGATTGATGTTGAGAGACATGCCAGAGATTTTATGGAAGCTGCCAAAAAGCTTCAGCTCTATTTTATTGGTCTGCAACGTGAGGATCTGCCCACGAATGCTGAAAAACTCAGAAAGGAGATTGCTGTGATGGAAGAGGAGTTGAAGGTAAAGAATGAGATTATCAAGAAGAATGAAAGGTTAATCCAAGGGTGGAAAAAGGAGCTGAGAGACCAATTGGACAGACACAATACTGAGCTCGAGAAAGTGTAG

Protein Analysis

191

Amino Acids

22.19

Weight (kDa)

5.35

Isoelectric Point (pI)

59.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med28 PF11594 80 - 177 2.7e-23 Mediator complex subunit 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 311
AclWI GGATC 1 cut(s) 417
AcuI CTGAAG 1 cut(s) 362
AgsI TTSAA 3 cut(s) 148, 293, 469
AjnI CCWGG 2 cut(s) 44, 192
AleI CACNNNNGTG 1 cut(s) 195
AluBI AGCT 7 cut(s) 132, 289, 365, 376, 382, 529, 562
AluI AGCT 7 cut(s) 132, 289, 365, 376, 382, 529, 562
Alw21I GWGCWC 1 cut(s) 564
Alw26I GTCTC 3 cut(s) 324, 334, 528
AlwI GGATC 1 cut(s) 417
Ama87I CYCGRG 1 cut(s) 563
ApeKI GCWGC 3 cut(s) 187, 257, 365
AspS9I GGNCC 1 cut(s) 119
AsuHPI GGTGA 2 cut(s) 209, 300
AvaI CYCGRG 1 cut(s) 563
AvaII GGWCC 1 cut(s) 119
BanII GRGCYC 1 cut(s) 564
BbsI GAAGAC 2 cut(s) 96, 132
Bbv12I GWGCWC 1 cut(s) 564
BbvI GCAGC 3 cut(s) 199, 244, 352
BccI CCATC 2 cut(s) 238, 448
BciT130I CCWGG 2 cut(s) 46, 194
BcoDI GTCTC 3 cut(s) 324, 334, 528
BisI GCNGC 3 cut(s) 188, 258, 366
BlsI GCNGC 3 cut(s) 189, 259, 367
Bme1390I CCNGG 2 cut(s) 46, 194
Bme18I GGWCC 1 cut(s) 119
BmeT110I CYCGRG 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 119
BmrFI CCNGG 2 cut(s) 46, 194
BmsI GCATC 3 cut(s) 22, 190, 198
BpiI GAAGAC 2 cut(s) 96, 132
BplI GAGNNNNNCTC 2 cut(s) 68, 100
BsaI GGTCTC 1 cut(s) 528
BsaJI CCNNGG 2 cut(s) 193, 511
BsaXI ACNNNNNCTCC 2 cut(s) 63, 93
BseBI CCWGG 2 cut(s) 46, 194
BseDI CCNNGG 2 cut(s) 193, 511
BseGI GGATG 2 cut(s) 37, 229
BseMII CTCAG 4 cut(s) 165, 448, 521, 549
BseRI GAGGAG 2 cut(s) 89, 476
BseXI GCAGC 3 cut(s) 199, 244, 352
BsiHKAI GWGCWC 1 cut(s) 564
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 1 cut(s) 117
BsmAI GTCTC 3 cut(s) 324, 334, 528
BsmBI CGTCTC 1 cut(s) 324
BsmI GAATGC 1 cut(s) 427
Bso31I GGTCTC 1 cut(s) 528
BsoBI CYCGRG 1 cut(s) 563
Bsp1286I GDGCHC 1 cut(s) 564
Bsp143I GATC 1 cut(s) 409
BspACI CCGC 1 cut(s) 311
BspCNI CTCAG 4 cut(s) 166, 447, 522, 550
BspPI GGATC 1 cut(s) 417
BspTNI GGTCTC 1 cut(s) 528
BssECI CCNNGG 2 cut(s) 193, 511
BssMI GATC 1 cut(s) 409
BssT1I CCWWGG 1 cut(s) 511
Bst2UI CCWGG 2 cut(s) 46, 194
Bst4CI ACNGT 1 cut(s) 305
Bst6I CTCTTC 1 cut(s) 453
BstC8I GCNNGC 2 cut(s) 275, 279
BstDEI CTNAG 4 cut(s) 174, 434, 530, 558
BstF5I GGATG 2 cut(s) 37, 229
BstKTI GATC 1 cut(s) 412
BstMAI GTCTC 3 cut(s) 324, 334, 528
BstMBI GATC 1 cut(s) 409
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 2 cut(s) 46, 194
BstNSI RCATGY 2 cut(s) 240, 347
BstSCI CCNGG 2 cut(s) 44, 192
BstV1I GCAGC 3 cut(s) 199, 244, 352
BstV2I GAAGAC 2 cut(s) 96, 132
BstX2I RGATCY 1 cut(s) 409
BstYI RGATCY 1 cut(s) 409
BtsCI GGATG 2 cut(s) 37, 229
Cac8I GCNNGC 2 cut(s) 275, 279
Cfr13I GGNCC 1 cut(s) 119
CsiI ACCWGGT 1 cut(s) 44
CviAII CATG 4 cut(s) 229, 237, 270, 344
DdeI CTNAG 4 cut(s) 174, 434, 530, 558
DpnI GATC 1 cut(s) 411
DpnII GATC 1 cut(s) 409
Eam1104I CTCTTC 1 cut(s) 453
EarI CTCTTC 1 cut(s) 453
Ecl136II GAGCTC 1 cut(s) 562
Eco130I CCWWGG 1 cut(s) 511
Eco24I GRGCYC 1 cut(s) 564
Eco31I GGTCTC 1 cut(s) 528
Eco47I GGWCC 1 cut(s) 119
Eco53kI GAGCTC 1 cut(s) 562
Eco57I CTGAAG 1 cut(s) 362
Eco88I CYCGRG 1 cut(s) 563
EcoICRI GAGCTC 1 cut(s) 562
EcoRII CCWGG 2 cut(s) 44, 192
EcoT14I CCWWGG 1 cut(s) 511
EcoT38I GRGCYC 1 cut(s) 564
ErhI CCWWGG 1 cut(s) 511
Esp3I CGTCTC 1 cut(s) 324
FaeI CATG 4 cut(s) 232, 240, 273, 347
FatI CATG 4 cut(s) 228, 236, 269, 343
FauNDI CATATG 1 cut(s) 53
Fnu4HI GCNGC 3 cut(s) 188, 258, 366
FokI GGATG 2 cut(s) 44, 236
FriOI GRGCYC 1 cut(s) 564
Fsp4HI GCNGC 3 cut(s) 188, 258, 366
GluI GCNGC 3 cut(s) 188, 258, 366
HapII CCGG 1 cut(s) 117
Hin1II CATG 4 cut(s) 232, 240, 273, 347
HindIII AAGCTT 1 cut(s) 374
HinfI GANTC 1 cut(s) 83
HpaII CCGG 1 cut(s) 117
HphI GGTGA 2 cut(s) 209, 300
Hpy166II GTNNAC 2 cut(s) 82, 308
Hpy188I TCNGA 3 cut(s) 88, 327, 437
Hpy188III TCNNGA 3 cut(s) 29, 490, 565
Hpy8I GTNNAC 2 cut(s) 82, 308
HpyAV CCTTC 3 cut(s) 35, 97, 463
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4IV ACGT 1 cut(s) 403
HpyCH4V TGCA 3 cut(s) 203, 211, 400
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 4 cut(s) 174, 434, 530, 558
HpySE526I ACGT 1 cut(s) 403
Hsp92II CATG 4 cut(s) 232, 240, 273, 347
Kzo9I GATC 1 cut(s) 409
LmnI GCTCC 1 cut(s) 526
Lsp1109I GCAGC 3 cut(s) 199, 244, 352
LweI GCATC 3 cut(s) 22, 190, 198
MabI ACCWGGT 1 cut(s) 44
MaeII ACGT 1 cut(s) 403
MalI GATC 1 cut(s) 411
MboI GATC 1 cut(s) 409
MboII GAAGA 4 cut(s) 101, 137, 470, 505
MfeI CAATTG 1 cut(s) 539
MflI RGATCY 1 cut(s) 409
MhlI GDGCHC 1 cut(s) 564
MluCI AATT 1 cut(s) 539
MlyI GAGTC 1 cut(s) 77
MnlI CCTC 6 cut(s) 67, 70, 151, 247, 400, 454
MseI TTAA 1 cut(s) 506
MslI CAYNNNNRTG 1 cut(s) 195
MspI CCGG 1 cut(s) 117
MspR9I CCNGG 2 cut(s) 46, 194
MunI CAATTG 1 cut(s) 539
Mva1269I GAATGC 1 cut(s) 427
MvaI CCWGG 2 cut(s) 46, 194
MwoI GCNNNNNNNGC 1 cut(s) 254
NdeI CATATG 1 cut(s) 53
NdeII GATC 1 cut(s) 409
NlaIII CATG 4 cut(s) 232, 240, 273, 347
NspI RCATGY 2 cut(s) 240, 347
OliI CACNNNNGTG 1 cut(s) 195
PaeR7I CTCGAG 1 cut(s) 563
PctI GAATGC 1 cut(s) 427
PkrI GCNGC 3 cut(s) 189, 259, 367
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
Psp124BI GAGCTC 1 cut(s) 564
Psp6I CCWGG 2 cut(s) 44, 192
PspGI CCWGG 2 cut(s) 44, 192
PspPI GGNCC 1 cut(s) 119
PsuI RGATCY 1 cut(s) 409
RseI CAYNNNNRTG 1 cut(s) 195
SacI GAGCTC 1 cut(s) 564
SaqAI TTAA 1 cut(s) 506
SatI GCNGC 3 cut(s) 188, 258, 366
Sau3AI GATC 1 cut(s) 409
Sau96I GGNCC 1 cut(s) 119
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 2 cut(s) 46, 194
SduI GDGCHC 1 cut(s) 564
SexAI ACCWGGT 1 cut(s) 44
SfaNI GCATC 3 cut(s) 22, 190, 198
Sfr274I CTCGAG 1 cut(s) 563
SinI GGWCC 1 cut(s) 119
SlaI CTCGAG 1 cut(s) 563
SmiMI CAYNNNNRTG 1 cut(s) 195
SmlI CTYRAG 1 cut(s) 563
SmoI CTYRAG 1 cut(s) 563
Sse9I AATT 1 cut(s) 539
SsiI CCGC 1 cut(s) 311
SspI AATATT 1 cut(s) 97
SstI GAGCTC 1 cut(s) 564
StyD4I CCNGG 2 cut(s) 44, 192
StyI CCWWGG 1 cut(s) 511
TaaI ACNGT 1 cut(s) 305
TaiI ACGT 1 cut(s) 406
TaqI TCGA 1 cut(s) 564
TasI AATT 1 cut(s) 539
Tru1I TTAA 1 cut(s) 506
Tru9I TTAA 1 cut(s) 506
TseI GCWGC 3 cut(s) 187, 257, 365
TspDTI ATGAA 2 cut(s) 40, 513
VpaK11BI GGWCC 1 cut(s) 119
XceI RCATGY 2 cut(s) 240, 347
XcmI CCANNNNNNNNNTGG 1 cut(s) 355
XhoI CTCGAG 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.