Rh7AG470300

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0005185
Physical Location & Seq
Reverse (-)
65102948 .. 65107222
4275 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG470300.1

Sequence Viewer

Length: 615 bp
ATGGTGGTGGAGGATGTGGAGATTGACTGTGGTGGTGAGGTTTCAACGAGGGAGTTTAGGAGGCGTTTGAGGTTTAGAAGAGACAATCTTTGTTATGCTGGAAGCCACGGCTTGGATATATCGACCCCATCAGCCTCTTCAAAATATAGTAACCATAAGCATCAATCTAGAACCACTGATGGAAAATCAAACTTGGCCTTGGACACCGGTAGTGACATAACATGGGTGCAATGTGTCCAAGGCCAAGTTCGACCGCAGGAGATCCGCTACGTACCAGCCTATGCTTTAGATGATCCGTTATGTTCTGGTCTTTCACATGATCATCGTGTCGGAAACGAGCCTTGTTCCTATTTGGCAGAATACCTTGACAGCCAGGCTAGTGAAGGTGAGGTTGGGTTTGACAACTTTACATTCTTTTCTGAAAATGGCCAGCCGCAACCAATTCCACATGTGGCACTTGGATCTGGACTCATCAATGATATTGACGTCGGGCCAGAAGGTCCACTTGGTAATCCGATTGGAGGAATTCTTGGGTTAGAGAAAGGTCGCCCCTCTTCTATCCTACAGCAACTGAGGTCTATCACACTTCAGAGGTTTTCTTACTGCCTTCCCTAG

Protein Analysis

204

Amino Acids

22.38

Weight (kDa)

5.18

Isoelectric Point (pI)

49.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 58 - 203 1.2e-19 Xylanase inhibitor N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 489
AccB7I CCANNNNNTGG 1 cut(s) 112
AciI CCGC 3 cut(s) 254, 265, 434
AclWI GGATC 3 cut(s) 256, 287, 469
AcoI YGGCCR 1 cut(s) 427
AcsI RAATTY 1 cut(s) 525
AcuI CTGAAG 1 cut(s) 572
AcyI GRCGYC 1 cut(s) 486
AfaI GTAC 1 cut(s) 273
AfiI CCNNNNNNNGG 2 cut(s) 112, 521
AflIII ACRYGT 1 cut(s) 448
AgeI ACCGGT 1 cut(s) 206
AgsI TTSAA 2 cut(s) 45, 141
AjnI CCWGG 1 cut(s) 372
AjuI GAANNNNNNNTTGG 6 cut(s) 231, 263, 375, 407, 489, 521
Alw26I GTCTC 1 cut(s) 75
AlwI GGATC 3 cut(s) 256, 287, 469
AlwNI CAGNNNCTG 1 cut(s) 571
AoxI GGCC 4 cut(s) 195, 241, 427, 491
ApoI RAATTY 1 cut(s) 525
AsiGI ACCGGT 1 cut(s) 206
AspS9I GGNCC 2 cut(s) 491, 500
AsuHPI GGTGA 2 cut(s) 47, 398
AvaII GGWCC 1 cut(s) 500
BalI TGGCCA 1 cut(s) 429
BccI CCATC 2 cut(s) 136, 173
BceAI ACGGC 1 cut(s) 124
BciT130I CCWGG 1 cut(s) 374
BclI TGATCA 1 cut(s) 319
BcoDI GTCTC 1 cut(s) 75
BfaI CTAG 3 cut(s) 168, 378, 613
BfmI CTRYAG 1 cut(s) 563
BisI GCNGC 1 cut(s) 434
BlsI GCNGC 1 cut(s) 435
Bme1390I CCNGG 1 cut(s) 374
Bme18I GGWCC 1 cut(s) 500
BmgT120I GGNCC 2 cut(s) 491, 500
BmrFI CCNGG 1 cut(s) 374
BmsI GCATC 1 cut(s) 169
BsaAI YACGTR 1 cut(s) 271
BsaHI GRCGYC 1 cut(s) 486
BsaJI CCNNGG 3 cut(s) 106, 198, 238
BsaWI WCCGGW 1 cut(s) 206
Bsc4I CCNNNNNNNGG 2 cut(s) 112, 521
Bse118I RCCGGY 1 cut(s) 206
Bse3DI GCAATG 1 cut(s) 236
BseBI CCWGG 1 cut(s) 374
BseDI CCNNGG 3 cut(s) 106, 198, 238
BseGI GGATG 1 cut(s) 19
BseLI CCNNNNNNNGG 2 cut(s) 112, 521
BseMI GCAATG 1 cut(s) 236
BseMII CTCAG 1 cut(s) 563
Bsh1285I CGRYCG 1 cut(s) 254
BshFI GGCC 4 cut(s) 197, 243, 429, 493
BshTI ACCGGT 1 cut(s) 206
BsiEI CGRYCG 1 cut(s) 254
BsiSI CCGG 1 cut(s) 207
BslI CCNNNNNNNGG 2 cut(s) 112, 521
BsmAI GTCTC 1 cut(s) 75
BsnI GGCC 4 cut(s) 197, 243, 429, 493
Bsp143I GATC 4 cut(s) 261, 292, 319, 461
BspACI CCGC 3 cut(s) 254, 265, 434
BspANI GGCC 4 cut(s) 197, 243, 429, 493
BspCNI CTCAG 1 cut(s) 564
BspPI GGATC 3 cut(s) 256, 287, 469
BsrDI GCAATG 1 cut(s) 236
BsrFI RCCGGY 1 cut(s) 206
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 3 cut(s) 106, 198, 238
BssMI GATC 4 cut(s) 261, 292, 319, 461
BssNI GRCGYC 1 cut(s) 486
BssT1I CCWWGG 2 cut(s) 198, 238
Bst2UI CCWGG 1 cut(s) 374
Bst4CI ACNGT 1 cut(s) 29
Bst6I CTCTTC 3 cut(s) 73, 142, 559
BstACI GRCGYC 1 cut(s) 486
BstBAI YACGTR 1 cut(s) 271
BstC8I GCNNGC 1 cut(s) 431
BstDEI CTNAG 1 cut(s) 572
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 1 cut(s) 19
BstKTI GATC 4 cut(s) 264, 295, 322, 464
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 4 cut(s) 261, 292, 319, 461
BstMCI CGRYCG 1 cut(s) 254
BstNI CCWGG 1 cut(s) 374
BstNSI RCATGY 1 cut(s) 452
BstSCI CCNGG 1 cut(s) 372
BstSFI CTRYAG 1 cut(s) 563
BstSNI TACGTA 1 cut(s) 271
BstX2I RGATCY 2 cut(s) 261, 461
BstYI RGATCY 2 cut(s) 261, 461
BsuRI GGCC 4 cut(s) 197, 243, 429, 493
BtgI CCRYGG 1 cut(s) 106
BtsCI GGATG 1 cut(s) 19
BtsIMutI CAGTG 1 cut(s) 174
Cac8I GCNNGC 1 cut(s) 431
CaiI CAGNNNCTG 1 cut(s) 571
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 2 cut(s) 491, 500
Csp6I GTAC 1 cut(s) 272
CspAI ACCGGT 1 cut(s) 206
CviAII CATG 3 cut(s) 222, 317, 449
CviQI GTAC 1 cut(s) 272
DdeI CTNAG 1 cut(s) 572
DpnI GATC 4 cut(s) 263, 294, 321, 463
DpnII GATC 4 cut(s) 261, 292, 319, 461
EaeI YGGCCR 1 cut(s) 427
Eam1104I CTCTTC 3 cut(s) 73, 142, 559
EarI CTCTTC 3 cut(s) 73, 142, 559
Eco105I TACGTA 1 cut(s) 271
Eco130I CCWWGG 2 cut(s) 198, 238
Eco47I GGWCC 1 cut(s) 500
Eco57I CTGAAG 1 cut(s) 572
EcoRI GAATTC 1 cut(s) 525
EcoRII CCWGG 1 cut(s) 372
EcoT14I CCWWGG 2 cut(s) 198, 238
ErhI CCWWGG 2 cut(s) 198, 238
FaeI CATG 3 cut(s) 225, 320, 452
FalI AAGNNNNNCTT 2 cut(s) 489, 521
FatI CATG 3 cut(s) 221, 316, 448
FbaI TGATCA 1 cut(s) 319
Fnu4HI GCNGC 1 cut(s) 434
FokI GGATG 1 cut(s) 26
Fsp4HI GCNGC 1 cut(s) 434
FspBI CTAG 3 cut(s) 168, 378, 613
GluI GCNGC 1 cut(s) 434
HaeIII GGCC 4 cut(s) 197, 243, 429, 493
HapII CCGG 1 cut(s) 207
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 3 cut(s) 225, 320, 452
HinfI GANTC 1 cut(s) 468
HpaII CCGG 1 cut(s) 207
HphI GGTGA 2 cut(s) 47, 398
Hpy166II GTNNAC 1 cut(s) 503
Hpy188I TCNGA 4 cut(s) 332, 421, 516, 591
Hpy188III TCNNGA 2 cut(s) 168, 465
Hpy8I GTNNAC 1 cut(s) 503
Hpy99I CGWCG 1 cut(s) 491
HpyAV CCTTC 2 cut(s) 377, 491
HpyCH4III ACNGT 1 cut(s) 29
HpyCH4IV ACGT 2 cut(s) 270, 486
HpyCH4V TGCA 1 cut(s) 229
HpyF3I CTNAG 1 cut(s) 572
HpySE526I ACGT 2 cut(s) 270, 486
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 3 cut(s) 225, 320, 452
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 4 cut(s) 261, 292, 319, 461
LweI GCATC 1 cut(s) 169
MaeI CTAG 3 cut(s) 168, 378, 613
MaeII ACGT 2 cut(s) 270, 486
MaeIII GTNAC 2 cut(s) 149, 212
MalI GATC 4 cut(s) 263, 294, 321, 463
MboI GATC 4 cut(s) 261, 292, 319, 461
MboII GAAGA 3 cut(s) 90, 129, 546
MflI RGATCY 2 cut(s) 261, 461
MlsI TGGCCA 1 cut(s) 429
MluCI AATT 2 cut(s) 441, 525
MluNI TGGCCA 1 cut(s) 429
MlyI GAGTC 1 cut(s) 462
MmeI TCCRAC 1 cut(s) 310
Mox20I TGGCCA 1 cut(s) 429
MscI TGGCCA 1 cut(s) 429
Msp20I TGGCCA 1 cut(s) 429
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 374
MvaI CCWGG 1 cut(s) 374
NdeII GATC 4 cut(s) 261, 292, 319, 461
NlaIII CATG 3 cut(s) 225, 320, 452
NmuCI GTSAC 1 cut(s) 212
NspI RCATGY 1 cut(s) 452
PciI ACATGT 1 cut(s) 448
PflMI CCANNNNNTGG 1 cut(s) 112
PinAI ACCGGT 1 cut(s) 206
PkrI GCNGC 1 cut(s) 435
PleI GAGTC 1 cut(s) 462
PpsI GAGTC 1 cut(s) 462
Ppu21I YACGTR 1 cut(s) 271
PscI ACATGT 1 cut(s) 448
Psp6I CCWGG 1 cut(s) 372
PspGI CCWGG 1 cut(s) 372
PspPI GGNCC 2 cut(s) 491, 500
PstNI CAGNNNCTG 1 cut(s) 571
PsuI RGATCY 2 cut(s) 261, 461
RsaI GTAC 1 cut(s) 273
RsaNI GTAC 1 cut(s) 272
SatI GCNGC 1 cut(s) 434
Sau3AI GATC 4 cut(s) 261, 292, 319, 461
Sau96I GGNCC 2 cut(s) 491, 500
SchI GAGTC 1 cut(s) 462
ScrFI CCNGG 1 cut(s) 374
SfaNI GCATC 1 cut(s) 169
SfcI CTRYAG 1 cut(s) 563
SinI GGWCC 1 cut(s) 500
SnaBI TACGTA 1 cut(s) 271
Sse9I AATT 2 cut(s) 441, 525
SsiI CCGC 3 cut(s) 254, 265, 434
SspMI CTAG 3 cut(s) 168, 378, 613
StyD4I CCNGG 1 cut(s) 372
StyI CCWWGG 2 cut(s) 198, 238
TaaI ACNGT 1 cut(s) 29
TaiI ACGT 2 cut(s) 273, 489
TaqI TCGA 2 cut(s) 122, 250
TasI AATT 2 cut(s) 441, 525
TauI GCSGC 1 cut(s) 436
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 1 cut(s) 212
Tsp45I GTSAC 1 cut(s) 212
TspGWI ACGGA 1 cut(s) 285
TspRI CASTG 1 cut(s) 181
Van91I CCANNNNNTGG 1 cut(s) 112
VpaK11BI GGWCC 1 cut(s) 500
XapI RAATTY 1 cut(s) 525
XbaI TCTAGA 1 cut(s) 167
XceI RCATGY 1 cut(s) 452
XspI CTAG 3 cut(s) 168, 378, 613
ZraI GACGTC 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.