Rw3G022470

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002343
Physical Location & Seq
Reverse (-)
27558228 .. 27561582
3355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G022470.1

Sequence Viewer

Length: 1569 bp
ATGCATATAGCAACGTCTCCTAGAAAATCCTTCCAGGCCTTCAAAAGGAAGTATCTAAGCCAGAGATGTGCTGCCTTGCGTACCATGATGTTGGCTGGTAAAGATACTGGCGAGGTGGATATTGAACTTTTGAAGGTTACTCACTTACGGAAGATGGGCCGCTATACTACAGGGTTTATGTTGTTAGGTTCTTCTTCAAAGCGAAGGAAGAAACGGAGGCATTATCAATTGTTCAGCCGTAATGGAGTTATAGTTTCTATATGTACGAGGACTCTTAAGGGTAACAAGATGGTTGTTCGCTGGTTTCACAAAGTTGATGAGGTTGGTATGGTCTTGCCTCTCAATTTTAATGATAGAGAGATTTTCTTCTCTCTCTGTCACCAACATCTCAGTATTGAATGCATTGATGGATTGGCTACTGTCCTCAGCCCCGTGCATTTTGAGAAATTTCTGAAAGAGGCGAAGCAAACACAGCCGGAACCCTTTGTATGCCATAAACAGTTTGAAAATGATGATGTAAAGACCTTTGACATTACTCAAGTTGAAGGTTATTGGAAACAGGAGATACTTAGATACATGCACACAGTTATCCCTTCAAAGGTTCATGTCAGTTCTCAACAATCTGTTAATGAGTTGAAAGTGGAAGAAATTGCTCATGCCAATGATGATATGGATGTTGAAGGCATTGAAAGAAGGGTGTCACTGGCTGCAGGATGTGGGGAAGCAGGCTATACAAGTAAATATGGGACTGATATGCATAAAGGTTCTGATGTTATTTTACCTTGTCCAGAGGTGAAACAAACCCTGCCGCAGTATCTAGCAGAAGGTTCCCAGGAAGTCCTGTCCCAAGATAGTGGCATTAGAGGGTGTTGGTTTAGAGCATTGATTATCAAGAAGCATAAAGATAAAGTTAAGGTGCAGTATCAAGATATTCAGGATGCAGCTGATGAAGCTAACAAACTTGAGGAATGGATTTTAGCATCAAAGATTTCAGCTCCTGATCAATTGGGTCATGATGGTTGGTGGGAAGGCATAGTACTTCAAAAGGAATATGAAGACAGACTTCATGTATACTTCCCAAATCAGGTTTTGATTGCGGGGCGTAGTGAAGTGCGACATTCTCAAGAGTGGCTGGGGAAAGATTGGGCTTATATGAAGGAGAGGCCAGACCTTGTGGTCTCAATATCAAGTACTTTAGAAACCAAGAAAACTGTTGGAAACTCATTTGATGGCATGTTAGGACTGTCTGAAATCAGTGATAGCAGAGAGTTGAAGAAACTTGAAACATCTGCTCTTTCCTCTGATAATGACAAGGATGAAAAAGTCAATGAAGTCAATAAGCCTAAGTGGAAGTCTTTGAGGAAGAGAAGACATGGTAATGGAAGCTCTGCCCGAAAGCTGCATTTTGGTAGTGATGGTAAAAGCACCTCTGGCCTCATGGGATCAAAATCTTGTGGAAGATATTTGATTCCATCATCTCTCATGGTTGACCACGAGAACTGCAAATATTTTGGGGATTCTCTTTTCAGTTCGTCAGTTGTGCCGCCTCTAACAAGCATAGTTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet BAH

Protein Analysis

522

Amino Acids

59.34

Weight (kDa)

8.84

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 279 - 330 9.5e-15 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1175
AccI GTMKAC 1 cut(s) 1071
AciI CCGC 4 cut(s) 160, 809, 1097, 1544
AclWI GGATC 1 cut(s) 1450
AcsI RAATTY 1 cut(s) 446
AfaI GTAC 4 cut(s) 82, 265, 1038, 1192
AfiI CCNNNNNNNGG 3 cut(s) 45, 598, 1084
AflII CTTAAG 1 cut(s) 275
AjnI CCWGG 2 cut(s) 33, 831
AluBI AGCT 5 cut(s) 944, 953, 995, 1386, 1399
AluI AGCT 5 cut(s) 944, 953, 995, 1386, 1399
Alw26I GTCTC 2 cut(s) 21, 1183
AlwI GGATC 1 cut(s) 1450
AlwNI CAGNNNCTG 1 cut(s) 998
AoxI GGCC 4 cut(s) 36, 157, 1163, 1432
ApeKI GCWGC 4 cut(s) 71, 707, 941, 1399
ApoI RAATTY 1 cut(s) 446
AspS9I GGNCC 1 cut(s) 157
AsuHPI GGTGA 2 cut(s) 371, 805
BarI GAAGNNNNNNTAC 2 cut(s) 1020, 1052
BauI CACGAG 1 cut(s) 1493
BbsI GAAGAC 2 cut(s) 1062, 1375
BbvCI CCTCAGC 1 cut(s) 425
BbvI GCAGC 4 cut(s) 58, 694, 953, 1386
BccI CCATC 7 cut(s) 148, 283, 401, 1010, 1223, 1409, 1480
BceAI ACGGC 1 cut(s) 222
BciT130I CCWGG 2 cut(s) 35, 833
BclI TGATCA 1 cut(s) 1000
BcoDI GTCTC 2 cut(s) 21, 1183
BfaI CTAG 2 cut(s) 21, 818
BfmI CTRYAG 2 cut(s) 168, 708
BfrI CTTAAG 1 cut(s) 275
BisI GCNGC 7 cut(s) 72, 160, 708, 809, 942, 1400, 1544
BlsI GCNGC 7 cut(s) 73, 161, 709, 810, 943, 1401, 1545
BmcAI AGTACT 2 cut(s) 1038, 1192
Bme1390I CCNGG 2 cut(s) 35, 833
BmgT120I GGNCC 1 cut(s) 157
BmiI GGNNCC 2 cut(s) 480, 829
BmrFI CCNGG 2 cut(s) 35, 833
BmsI GCATC 2 cut(s) 928, 989
BpiI GAAGAC 2 cut(s) 1062, 1375
Bpu10I CCTNAGC 1 cut(s) 425
BpuEI CTTGAG 3 cut(s) 522, 983, 1107
BsaBI GATNNNNATC 1 cut(s) 1447
BsaI GGTCTC 1 cut(s) 1183
BsaJI CCNNGG 1 cut(s) 831
BsaXI ACNNNNNCTCC 2 cut(s) 237, 267
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 598, 1084
Bse1I ACTGG 2 cut(s) 112, 708
Bse8I GATNNNNATC 1 cut(s) 1447
BseBI CCWGG 2 cut(s) 35, 833
BseDI CCNNGG 1 cut(s) 831
BseGI GGATG 4 cut(s) 679, 719, 943, 1321
BseJI GATNNNNATC 1 cut(s) 1447
BseLI CCNNNNNNNGG 3 cut(s) 45, 598, 1084
BseMII CTCAG 2 cut(s) 403, 439
BseNI ACTGG 2 cut(s) 112, 708
BseXI GCAGC 4 cut(s) 58, 694, 953, 1386
BseYI CCCAGC 1 cut(s) 1132
BsgI GTGCAG 1 cut(s) 938
BshFI GGCC 4 cut(s) 38, 159, 1165, 1434
BsiSI CCGG 1 cut(s) 476
BslFI GGGAC 2 cut(s) 760, 829
BslI CCNNNNNNNGG 3 cut(s) 45, 598, 1084
BsmAI GTCTC 2 cut(s) 21, 1183
BsmBI CGTCTC 1 cut(s) 21
BsmFI GGGAC 2 cut(s) 760, 829
BsmI GAATGC 1 cut(s) 404
BsnI GGCC 4 cut(s) 38, 159, 1165, 1434
Bso31I GGTCTC 1 cut(s) 1183
Bsp143I GATC 2 cut(s) 1000, 1442
BspACI CCGC 4 cut(s) 160, 809, 1097, 1544
BspANI GGCC 4 cut(s) 38, 159, 1165, 1434
BspCNI CTCAG 2 cut(s) 402, 438
BspHI TCATGA 1 cut(s) 1012
BspLI GGNNCC 2 cut(s) 480, 829
BspMAI CTGCAG 1 cut(s) 712
BspPI GGATC 1 cut(s) 1450
BspTI CTTAAG 1 cut(s) 275
BspTNI GGTCTC 1 cut(s) 1183
BsrI ACTGG 2 cut(s) 112, 708
BssECI CCNNGG 1 cut(s) 831
BssMI GATC 2 cut(s) 1000, 1442
BssNAI GTATAC 1 cut(s) 1072
BssSI CACGAG 1 cut(s) 1493
Bst1107I GTATAC 1 cut(s) 1072
Bst2BI CACGAG 1 cut(s) 1493
Bst2UI CCWGG 2 cut(s) 35, 833
Bst4CI ACNGT 5 cut(s) 421, 501, 586, 1213, 1245
Bst6I CTCTTC 1 cut(s) 1358
BstAFI CTTAAG 1 cut(s) 275
BstC8I GCNNGC 1 cut(s) 727
BstDEI CTNAG 5 cut(s) 56, 389, 425, 569, 1344
BstENI CCTNNNNNAGG 1 cut(s) 43
BstF5I GGATG 4 cut(s) 679, 719, 943, 1321
BstKTI GATC 2 cut(s) 1003, 1445
BstMAI GTCTC 2 cut(s) 21, 1183
BstMBI GATC 2 cut(s) 1000, 1442
BstMWI GCNNNNNNNGC 3 cut(s) 472, 950, 1431
BstNI CCWGG 2 cut(s) 35, 833
BstNSI RCATGY 2 cut(s) 580, 1237
BstSCI CCNGG 2 cut(s) 33, 831
BstSFI CTRYAG 2 cut(s) 168, 708
BstV1I GCAGC 4 cut(s) 58, 694, 953, 1386
BstV2I GAAGAC 2 cut(s) 1062, 1375
BstXI CCANNNNNNTGG 2 cut(s) 91, 854
BstZ17I GTATAC 1 cut(s) 1072
BsuRI GGCC 4 cut(s) 38, 159, 1165, 1434
BtsCI GGATG 4 cut(s) 679, 719, 943, 1321
BtsIMutI CAGTG 2 cut(s) 701, 1261
Cac8I GCNNGC 1 cut(s) 727
CaiI CAGNNNCTG 1 cut(s) 998
CciI TCATGA 1 cut(s) 1012
Cfr13I GGNCC 1 cut(s) 157
Csp6I GTAC 4 cut(s) 81, 264, 1037, 1191
CviQI GTAC 4 cut(s) 81, 264, 1037, 1191
DdeI CTNAG 5 cut(s) 56, 389, 425, 569, 1344
DpnI GATC 2 cut(s) 1002, 1444
DpnII GATC 2 cut(s) 1000, 1442
DrdI GACNNNNNNGTC 1 cut(s) 1175
DseDI GACNNNNNNGTC 1 cut(s) 1175
Eam1104I CTCTTC 1 cut(s) 1358
EarI CTCTTC 1 cut(s) 1358
Eco147I AGGCCT 1 cut(s) 38
Eco31I GGTCTC 1 cut(s) 1183
EcoNI CCTNNNNNAGG 1 cut(s) 43
EcoRII CCWGG 2 cut(s) 33, 831
EcoT22I ATGCAT 3 cut(s) 6, 404, 759
Esp3I CGTCTC 1 cut(s) 21
FalI AAGNNNNNCTT 2 cut(s) 1047, 1079
FaqI GGGAC 2 cut(s) 760, 829
FauI CCCGC 1 cut(s) 1090
FbaI TGATCA 1 cut(s) 1000
FblI GTMKAC 1 cut(s) 1071
Fnu4HI GCNGC 7 cut(s) 72, 160, 708, 809, 942, 1400, 1544
FokI GGATG 4 cut(s) 686, 726, 950, 1328
Fsp4HI GCNGC 7 cut(s) 72, 160, 708, 809, 942, 1400, 1544
FspBI CTAG 2 cut(s) 21, 818
GluI GCNGC 7 cut(s) 72, 160, 708, 809, 942, 1400, 1544
GsaI CCCAGC 1 cut(s) 1136
HaeIII GGCC 4 cut(s) 38, 159, 1165, 1434
HapII CCGG 1 cut(s) 476
HincII GTYRAC 1 cut(s) 1489
HindII GTYRAC 1 cut(s) 1489
HinfI GANTC 3 cut(s) 271, 1468, 1517
HpaII CCGG 1 cut(s) 476
HphI GGTGA 2 cut(s) 371, 805
Hpy166II GTNNAC 2 cut(s) 1072, 1489
Hpy188I TCNGA 4 cut(s) 453, 769, 1249, 1303
Hpy188III TCNNGA 7 cut(s) 788, 892, 926, 935, 998, 1013, 1124
Hpy8I GTNNAC 2 cut(s) 1072, 1489
HpyCH4III ACNGT 5 cut(s) 421, 501, 586, 1213, 1245
HpyCH4IV ACGT 1 cut(s) 14
HpyF10VI GCNNNNNNNGC 3 cut(s) 472, 950, 1431
HpyF3I CTNAG 5 cut(s) 56, 389, 425, 569, 1344
HpySE526I ACGT 1 cut(s) 14
Ksp22I TGATCA 1 cut(s) 1000
Kzo9I GATC 2 cut(s) 1000, 1442
LmnI GCTCC 1 cut(s) 1000
Lsp1109I GCAGC 4 cut(s) 58, 694, 953, 1386
LweI GCATC 2 cut(s) 928, 989
MaeI CTAG 2 cut(s) 21, 818
MaeII ACGT 1 cut(s) 14
MaeIII GTNAC 4 cut(s) 136, 281, 377, 699
MalI GATC 2 cut(s) 1002, 1444
MboI GATC 2 cut(s) 1000, 1442
MfeI CAATTG 2 cut(s) 227, 1004
MluCI AATT 5 cut(s) 227, 343, 446, 648, 1004
MlyI GAGTC 1 cut(s) 265
MmeI TCCRAC 1 cut(s) 1195
Mph1103I ATGCAT 3 cut(s) 6, 404, 759
MseI TTAA 4 cut(s) 276, 348, 627, 912
MslI CAYNNNNRTG 3 cut(s) 660, 1377, 1562
MspA1I CMGCKG 1 cut(s) 944
MspCI CTTAAG 1 cut(s) 275
MspI CCGG 1 cut(s) 476
MspR9I CCNGG 2 cut(s) 35, 833
MunI CAATTG 2 cut(s) 227, 1004
Mva1269I GAATGC 1 cut(s) 404
MvaI CCWGG 2 cut(s) 35, 833
MwoI GCNNNNNNNGC 3 cut(s) 472, 950, 1431
NdeII GATC 2 cut(s) 1000, 1442
NlaIV GGNNCC 2 cut(s) 480, 829
NmuCI GTSAC 2 cut(s) 377, 699
NsiI ATGCAT 3 cut(s) 6, 404, 759
NspI RCATGY 2 cut(s) 580, 1237
PagI TCATGA 1 cut(s) 1012
PceI AGGCCT 1 cut(s) 38
PctI GAATGC 1 cut(s) 404
PfeI GAWTC 2 cut(s) 1468, 1517
PkrI GCNGC 7 cut(s) 73, 161, 709, 810, 943, 1401, 1545
PleI GAGTC 1 cut(s) 265
PpsI GAGTC 1 cut(s) 265
Psp6I CCWGG 2 cut(s) 33, 831
PspFI CCCAGC 1 cut(s) 1132
PspGI CCWGG 2 cut(s) 33, 831
PspN4I GGNNCC 2 cut(s) 480, 829
PspPI GGNCC 1 cut(s) 157
PstI CTGCAG 1 cut(s) 712
PstNI CAGNNNCTG 1 cut(s) 998
PvuII CAGCTG 1 cut(s) 944
RsaI GTAC 4 cut(s) 82, 265, 1038, 1192
RsaNI GTAC 4 cut(s) 81, 264, 1037, 1191
RseI CAYNNNNRTG 3 cut(s) 660, 1377, 1562
SaqAI TTAA 4 cut(s) 276, 348, 627, 912
SatI GCNGC 7 cut(s) 72, 160, 708, 809, 942, 1400, 1544
Sau3AI GATC 2 cut(s) 1000, 1442
Sau96I GGNCC 1 cut(s) 157
ScaI AGTACT 2 cut(s) 1038, 1192
SchI GAGTC 1 cut(s) 265
ScrFI CCNGG 2 cut(s) 35, 833
SfaNI GCATC 2 cut(s) 928, 989
SfcI CTRYAG 2 cut(s) 168, 708
SmiMI CAYNNNNRTG 3 cut(s) 660, 1377, 1562
SmlI CTYRAG 4 cut(s) 275, 537, 962, 1122
SmoI CTYRAG 4 cut(s) 275, 537, 962, 1122
Sse9I AATT 5 cut(s) 227, 343, 446, 648, 1004
SseBI AGGCCT 1 cut(s) 38
SsiI CCGC 4 cut(s) 160, 809, 1097, 1544
SspI AATATT 1 cut(s) 1508
SspMI CTAG 2 cut(s) 21, 818
StuI AGGCCT 1 cut(s) 38
StyD4I CCNGG 2 cut(s) 33, 831
TaaI ACNGT 5 cut(s) 421, 501, 586, 1213, 1245
TaiI ACGT 1 cut(s) 17
TasI AATT 5 cut(s) 227, 343, 446, 648, 1004
TatI WGTACW 2 cut(s) 1036, 1190
TauI GCSGC 3 cut(s) 162, 811, 1546
TfiI GAWTC 2 cut(s) 1468, 1517
Tru1I TTAA 4 cut(s) 276, 348, 627, 912
Tru9I TTAA 4 cut(s) 276, 348, 627, 912
TscAI CASTG 2 cut(s) 708, 1261
TseFI GTSAC 2 cut(s) 377, 699
TseI GCWGC 4 cut(s) 71, 707, 941, 1399
Tsp45I GTSAC 2 cut(s) 377, 699
TspDTI ATGAA 7 cut(s) 593, 963, 1055, 1068, 1169, 1332, 1344
TspGWI ACGGA 2 cut(s) 163, 229
TspRI CASTG 2 cut(s) 708, 1261
Vha464I CTTAAG 1 cut(s) 275
XagI CCTNNNNNAGG 1 cut(s) 43
XapI RAATTY 1 cut(s) 446
XceI RCATGY 2 cut(s) 580, 1237
XcmI CCANNNNNNNNNTGG 1 cut(s) 667
XmiI GTMKAC 1 cut(s) 1071
XspI CTAG 2 cut(s) 21, 818
ZrmI AGTACT 2 cut(s) 1038, 1192
Zsp2I ATGCAT 3 cut(s) 6, 404, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.