Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002643

Physical Location & Seq

Genomic Coordinates Forward (+)

1899951 .. 1904897

Gene Structure 4947 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G06220.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2964 bp

ATGGAAAGTAGCTTGTATGATGAGTTTGGTAACTATGTTGGACCTGAGATTGAGTCTGACAGAGATAGTGATGATGAAGTAGAAGATGAAGATCTCCAAGATAAGCATCTTGAAGAAAATGGTTCCGATGGCGAACAAGGGCCTGGGGGTTCTAATGGCTGGATTACCACTATCAATGATGTTGAGATGGAGAACCAAATTGTTCTTCCTGAGGATAAGAAGTACTACCCTACTGCTGAGGAGGTTTATGGTGAGGATGTTGAGACCTTGGTTATGGATGAAGATGAGCAGCCTCTTGAGCAACCCATTATCAAACCTGTTAGAGATATTAGATTTGAGGTGGGGGTCAAAGATCAAGCAACGTATGTGTCAACACAGTTTCTTATCGGCCTCATGTCTAATCCTGCGCTTGTGAGGAATGTTGCTCTTGTGGGGCATCTACAGCACGGCAAAACTGTCTTCATGGATATGTTGGTAGAGCAGACGCATCATATGTCTACTTTTAATGCTAAAAACGAGAAGCATATGAAATATACAGACACACGAGTCGATGAGCAGGAGAGAAATATATCAATCAAGGCGGTTCCAATGTCTCTTGTCCTTGAGGACAGTAGATCCAAATCATACCTGTGCAATATCATGGATACCCCTGGACATGTCAATTTCTCTGACGAAATGACTGCTTCTTTAAGACTTGCTGATGGTGCTGTTCTGATTGTTGATGCTGCTGAAGGAGTGATGGTTAACACAGAGAGAGCTATACGGCATGCAATCCAGGACCATCTCCCTATTGTAGTTGTTATCAATAAGGTTGACAGGCTCATAACTGAGCTCAAACTACCTCCAAGGGATGCTTACTACAAGCTGAGGCATACAATTGAAGTCATTAATAACCACATATCTGCTGCTTCAACAACTGCTGGAGACTTACCTCTTATAGACCCTGCAGCTGGAAATGTATGTTTTGCCAGTGGTACAGCCGGATGGTCCTTCACATTACAATCTTTTGCTAAAATGTATGCCAAGCTTCATGGTGTGGCCATGGACGTGGATAAGTTTGCATCTCGACTTTGGGGAGATGTATATTATCACTCTGATACTAGGGTATTCAAGAGAAGTCCTCCAGTAGGTGGTGGAGAAAGAGCATTTGTTCAGTTTATTCTGGAGCCCCTATACAAGATATACAGCCAAGTGATAGGTGAACATAAGAAGAGTGTAGAGACCACCCTTGCAGAATTAGGAGTGACTCTGAGTAATAGTGCATATAAGCTGAACGTCAGACCTCTACTTAGGTTAGCCTGTAGTTCAGTGTTTGGTTCGGCATCAGGGTTCACTGATATGTTGGTAAAGCACATTCCTTCACCCAGAGAGGCTGCAGCTAGGAAGGTCGACCATTCATATACCGGAACCAAAGATTCCCCCATTTATGAATCAATGGTAGAATGTGATCCATCTGGACCTCTCATGGTTAATGTGACAAAGTTGTACCCCAAATCAGATACTAGTGTTTTTGATGTGTTTGGAAGAGTTTATAGTGGTAGGCTTCAAACAGGGCAAAGCGTACGTGTACTAGGAGAAGGGTATTCACCTGAGGATGAGGAGGACATGACTATAAAAGAAGTGACGAAGCTATGGATATATCAAGCTAGGTATAGAATACCAGTAAGCAGTGCCCCTCCCGGTTCCTGGGTTTTAATTGAAGGTGTCGATGCATCCATCATGAAAACTGCGACTCTGTGTAATGCAAGCTATGATGAAGATGTCTACATATTCCGGGCTCTCCAATTTAATACCCTCCCAGTAGTCAAGACTGCAACTGAGCCTTTGAATCCTAGTGAGCTGCCTAAAATGGTGGAAGGGCTTAGGAAAATTAGCAAAAGCTATCCCCTTGCGATTACCAAAGTTGAGGAGTCTGGGGAACATACTATTCTAGGCACAGGTGAGTTGTACTTGGATTCAATCATGAAGGACCTCAGGGAGCTCTATTCAGAGGTCGAAGTAAAGGTTGCAGATCCGGTGGTCTCCTTCTGTGAGACAGTGGTTGAATCTTCGTCGATGAAGTGTTTTGCTGAGACACCAAACAAAAAGAACAAAATAACTATGATTGCAGAGCCATTGGATAGAGGACTTGCAGAGGACATTGAGAATGGCGTTGTAAGCATTGACTGGAACAGAAAACAACTTGGTGACTTCTTCAGGACGAAATATGACTGGGATTTACTTGCTGCACGTTCCATCTGGGCTTTTGGCCCTGACAAACAGGGGCCAAACATATTATTGGATGACACCCTTCCAACTGAGGTGGACAGGAACTTGATGATGGCAGTGAAAGACTCCATTGTTCAAGGGTTCCAGTGGGGTGCTCGTGAAGGTCCCCTCTGTGATGAGCCCATCAGAAATGTAAAGTTCAAGATTGTTGATGCACGGATAGCACCAGAGCCATTGCATAGAGGATCTGGTCAGATGATTCCAACAGCAAGACGTGTTGCATATTCAGCTTTTCTAATGGCAACTCCAAGGCTAATGGAACCTGTCTACTATGTGGAGATACAAACACCAATCGACTGTGTAACTGCAATCTACACAGTTTTGTCACGTAGACGTGGGCATGTCACATCTGATGTTCCTCAACCCGGTACTCCAGCTTATATCGTGAAGGCCTTTCTGCCTGTGATAGAGTCCTTTGGGTTTGAGACAGATCTGAGGTACCACACTCAGGGACAAGCCTTTTGCCTATCTGTGTTTGATCACTGGGCCATTGTGCCAGGAGACCCTCTAGACAAAGCCATCCAGTTGCGCCCACTTGAGCCCGCTCCTATTCAGCATCTAGCTCGGGAATTCATGGTCAAGACCCGCCGTAGAAAGGGAATGAGCGAAGACGTCAGCGGAAATAAATTCTTTGATGAAGCGATGATGGTAGAGCTTGCCCAGCAAACAGGCGATCTCCATCTGCAGATGATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005730 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0007275 GO:0008150 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0010154 GO:0015030 GO:0016020 GO:0016604 GO:0016607 GO:0022414 GO:0030054 GO:0030312 GO:0030532 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043900 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045694 GO:0046540 GO:0048316 GO:0048367 GO:0048437 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0070013 GO:0071007 GO:0071013 GO:0071944 GO:0090567 GO:0097525 GO:0097526 GO:0099402 GO:0120114 GO:1902494 GO:1990904 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

EFG_C EFG_IV EFTUD2 GTP_EFTU GTP_EFTU_D2

Protein Analysis

987

Amino Acids

110.44

Weight (kDa)

5.05

Isoelectric Point (pI)

43.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EFTUD2	PF16004	4 - 118	1.4e-33	116 kDa U5 small nuclear ribonucleoprotein component N-terminus
GTP_EFTU	PF00009	138 - 332	2e-43	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	506 - 580	6.3e-09	Elongation factor Tu domain 2
EFG_IV	PF03764	723 - 837	9.2e-19	Elongation factor G, domain IV
EFG_C	PF00679	840 - 928	2.5e-22	Elongation factor G C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002643)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06220 AT1G06220 AT1G06220 AT5G25230 AT5G25230
fragaria_vesca	FvH4_2g24581 FvH4_7g03790 FvH4_7g03790 FvH4_7g03790
malus_domestica	MD02G1013600.v1.1 MD15G1160400.v1.1
prunus_persica	Prupe.3G036300_v2.0.a1
pyrus_communis	pycom02g01260 pycom15g14420
rosa_chinensis	RchiOBHm_Chr1g0327101 RchiOBHm_Chr1g0327121 RchiOBHm_Chr2g0127751
rosa_laevigata	RLG00000012029 RLG00000012030 RLG00000030043
rosa_multiflora	Rmu_sc0000795.1_g000050 Rmu_sc0004240.1_g000007
rosa_roxburghii	Rroxscaffold_4G00323330 Rroxscaffold_6G00399840 Rroxscaffold_6G00400180
rosa_rugosa	Rorug01G0063500
rosa_samantha	Rh1AG080100 Rh1AG080300 Rh1AG080700 Rh1AG080900 Rh1BG064100 Rh1CG078700 Rh1CG078800 Rh1DG085100 Rh1DG085200 Rh2AG323400 Rh2BG332600 Rh2DG350900
rosa_wichuraiana	Rw1G006400 Rw2G026250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	545
AatII	GACGTC	1 cut(s)	2883
Acc65I	GGTACC	1 cut(s)	2706
AccB1I	GGYRCC	1 cut(s)	2706
AccB7I	CCANNNNNTGG	1 cut(s)	1130
AccBSI	CCGCTC	1 cut(s)	2813
AccI	GTMKAC	5 cut(s)	497, 1389, 1764, 2535, 2599
AciI	CCGC	4 cut(s)	581, 2811, 2854, 2886
AclWI	GGATC	4 cut(s)	609, 1442, 2006, 2461
AcoI	YGGCCR	1 cut(s)	1038
AcsI	RAATTY	2 cut(s)	2837, 2894
AcuI	CTGAAG	2 cut(s)	750, 2179
AcyI	GRCGYC	1 cut(s)	2880
AfaI	GTAC	8 cut(s)	224, 976, 1487, 1563, 1569, 1949, 2638, 2708
AfiI	CCNNNNNNNGG	7 cut(s)	950, 1127, 1130, 1686, 2633, 2716, 2863
AflIII	ACRYGT	3 cut(s)	655, 1564, 2482
AhlI	ACTAGT	1 cut(s)	1502
AjiI	CACGTC	3 cut(s)	1048, 2483, 2603
AjnI	CCWGG	5 cut(s)	142, 649, 774, 1685, 2764
AjuI	GAANNNNNNNTTGG	2 cut(s)	189, 221
Alw21I	GWGCWC	3 cut(s)	834, 1983, 2365
Alw26I	GTCTC	9 cut(s)	257, 597, 918, 1214, 2026, 2027, 2066, 2687, 2763
AlwI	GGATC	4 cut(s)	609, 1442, 2006, 2461
Ama87I	CYCGRG	1 cut(s)	2832
AoxI	GGCC	7 cut(s)	140, 388, 1038, 2248, 2264, 2658, 2754
ApeKI	GCWGC	8 cut(s)	289, 725, 905, 947, 1373, 1376, 1840, 2225
ApoI	RAATTY	2 cut(s)	2837, 2894
ArsI	GACNNNNNNTTYG	2 cut(s)	2712, 2744
AseI	ATTAAT	1 cut(s)	888
Asp718I	GGTACC	1 cut(s)	2706
AspLEI	GCGC	2 cut(s)	409, 2799
AsuC2I	CCSGG	3 cut(s)	1680, 1775, 2634
AsuHPI	GGTGA	6 cut(s)	263, 1211, 1353, 1578, 1952, 2198
AvaI	CYCGRG	1 cut(s)	2832
AvaII	GGWCC	6 cut(s)	41, 778, 987, 1457, 1969, 2372
AxyI	CCTNAGG	3 cut(s)	210, 1590, 1973
BaeGI	GKGCMC	1 cut(s)	1675
BaeI	ACNNNNGTAYC	4 cut(s)	1089, 1122, 1491, 1524
BalI	TGGCCA	1 cut(s)	1040
BanI	GGYRCC	1 cut(s)	2706
BanII	GRGCYC	6 cut(s)	834, 1170, 1780, 1983, 2391, 2811
BauI	CACGAG	2 cut(s)	543, 2364
BbsI	GAAGAC	2 cut(s)	451, 2883
Bbv12I	GWGCWC	3 cut(s)	834, 1983, 2365
BbvCI	CCTCAGC	2 cut(s)	237, 866
BbvI	GCAGC	8 cut(s)	301, 712, 892, 959, 1360, 1388, 1827, 2212
BceAI	ACGGC	3 cut(s)	463, 779, 2841
BcgI	CGANNNNNNTGC	4 cut(s)	662, 696, 2813, 2847
BciT130I	CCWGG	5 cut(s)	144, 651, 776, 1687, 2766
BciVI	GTATCC	1 cut(s)	637
BclI	TGATCA	1 cut(s)	2746
BcnI	CCSGG	3 cut(s)	1680, 1775, 2634
BcoDI	GTCTC	9 cut(s)	257, 597, 918, 1214, 2026, 2027, 2066, 2687, 2763
BcuI	ACTAGT	1 cut(s)	1502
BfaI	CTAG	9 cut(s)	1101, 1380, 1503, 1571, 1647, 1833, 1931, 2777, 2828
BfmI	CTRYAG	5 cut(s)	440, 945, 1300, 1374, 2951
BfuI	GTATCC	1 cut(s)	637
BglII	AGATCT	2 cut(s)	91, 2698
BisI	GCNGC	8 cut(s)	290, 726, 906, 948, 1374, 1377, 1841, 2226
BlsI	GCNGC	8 cut(s)	291, 727, 907, 949, 1375, 1378, 1842, 2227
BmcAI	AGTACT	1 cut(s)	224
Bme1390I	CCNGG	8 cut(s)	144, 651, 776, 1680, 1687, 1775, 2634, 2766
Bme18I	GGWCC	6 cut(s)	41, 778, 987, 1457, 1969, 2372
BmeT110I	CYCGRG	1 cut(s)	2832
BmgBI	CACGTC	3 cut(s)	1048, 2483, 2603
BmrFI	CCNGG	8 cut(s)	144, 651, 776, 1680, 1687, 1775, 2634, 2766
BmrI	ACTGGG	3 cut(s)	1793, 2221, 2761
BmuI	ACTGGG	3 cut(s)	1793, 2221, 2761
BpiI	GAAGAC	2 cut(s)	451, 2883
BplI	GAGNNNNNCTC	2 cut(s)	1105, 1137
BpmI	CTGGAG	4 cut(s)	942, 1107, 1184, 2625
Bpu10I	CCTNAGC	3 cut(s)	237, 866, 1862
BpuEI	CTTGAG	3 cut(s)	317, 623, 2825
BpuMI	CCSGG	3 cut(s)	1680, 1775, 2634
BsaAI	YACGTR	2 cut(s)	1565, 2597
BsaBI	GATNNNNATC	4 cut(s)	90, 105, 619, 2946
BsaHI	GRCGYC	1 cut(s)	2880
BsaI	GGTCTC	4 cut(s)	257, 1214, 2026, 2763
BsaJI	CCNNGG	7 cut(s)	143, 267, 649, 845, 1041, 1686, 2516
BsaWI	WCCGGW	2 cut(s)	1403, 2014
BsaXI	ACNNNNNCTCC	2 cut(s)	726, 756
Bsc4I	CCNNNNNNNGG	7 cut(s)	950, 1127, 1130, 1686, 2633, 2716, 2863
Bse1I	ACTGG	9 cut(s)	969, 1124, 1661, 1799, 2171, 2216, 2353, 2756, 2791
Bse21I	CCTNAGG	3 cut(s)	210, 1590, 1973
Bse3DI	GCAATG	1 cut(s)	2441
Bse8I	GATNNNNATC	4 cut(s)	90, 105, 619, 2946
BseBI	CCWGG	5 cut(s)	144, 651, 776, 1687, 2766
BseDI	CCNNGG	7 cut(s)	143, 267, 649, 845, 1041, 1686, 2516
BseGI	GGATG	8 cut(s)	262, 283, 856, 989, 1600, 1712, 2287, 2787
BseJI	GATNNNNATC	4 cut(s)	90, 105, 619, 2946
BseLI	CCNNNNNNNGG	7 cut(s)	950, 1127, 1130, 1686, 2633, 2716, 2863
BseMI	GCAATG	1 cut(s)	2441
BseNI	ACTGG	9 cut(s)	969, 1124, 1661, 1799, 2171, 2216, 2353, 2756, 2791
BseRI	GAGGAG	3 cut(s)	254, 1613, 1922
BseSI	GKGCMC	1 cut(s)	1675
BseXI	GCAGC	8 cut(s)	301, 712, 892, 959, 1360, 1388, 1827, 2212
BseYI	CCCAGC	1 cut(s)	2928
BsgI	GTGCAG	1 cut(s)	2211
BshFI	GGCC	7 cut(s)	142, 390, 1040, 2250, 2266, 2660, 2756
BshNI	GGYRCC	1 cut(s)	2706
BsiHKAI	GWGCWC	3 cut(s)	834, 1983, 2365
BsiHKCI	CYCGRG	1 cut(s)	2832
BsiSI	CCGG	6 cut(s)	981, 1404, 1680, 1774, 2015, 2634
BsiWI	CGTACG	1 cut(s)	1561
BslFI	GGGAC	2 cut(s)	2358, 2733
BslI	CCNNNNNNNGG	7 cut(s)	950, 1127, 1130, 1686, 2633, 2716, 2863
BsmAI	GTCTC	9 cut(s)	257, 597, 918, 1214, 2026, 2027, 2066, 2687, 2763
BsmFI	GGGAC	2 cut(s)	2358, 2733
BsnI	GGCC	7 cut(s)	142, 390, 1040, 2250, 2266, 2660, 2756
Bso31I	GGTCTC	4 cut(s)	257, 1214, 2026, 2763
BsoBI	CYCGRG	1 cut(s)	2832
Bsp1286I	GDGCHC	8 cut(s)	834, 1170, 1675, 1780, 1983, 2365, 2391, 2811
Bsp143I	GATC	9 cut(s)	91, 352, 614, 1447, 2011, 2453, 2698, 2746, 2941
Bsp19I	CCATGG	1 cut(s)	1041
BspACI	CCGC	4 cut(s)	581, 2811, 2854, 2886
BspANI	GGCC	7 cut(s)	142, 390, 1040, 2250, 2266, 2660, 2756
BspHI	TCATGA	2 cut(s)	1719, 1962
BspMAI	CTGCAG	3 cut(s)	949, 1378, 2955
BspPI	GGATC	4 cut(s)	609, 1442, 2006, 2461
BspT107I	GGYRCC	1 cut(s)	2706
BspTNI	GGTCTC	4 cut(s)	257, 1214, 2026, 2763
BsrBI	CCGCTC	1 cut(s)	2813
BsrDI	GCAATG	1 cut(s)	2441
BsrI	ACTGG	9 cut(s)	969, 1124, 1661, 1799, 2171, 2216, 2353, 2756, 2791
BssECI	CCNNGG	7 cut(s)	143, 267, 649, 845, 1041, 1686, 2516
BssMI	GATC	9 cut(s)	91, 352, 614, 1447, 2011, 2453, 2698, 2746, 2941
BssNI	GRCGYC	1 cut(s)	2880
BssSI	CACGAG	2 cut(s)	543, 2364
BssT1I	CCWWGG	4 cut(s)	267, 845, 1041, 2516
Bst2BI	CACGAG	2 cut(s)	543, 2364
Bst2UI	CCWGG	5 cut(s)	144, 651, 776, 1687, 2766
Bst4CI	ACNGT	6 cut(s)	378, 457, 611, 2038, 2567, 2587
Bst6I	CTCTTC	2 cut(s)	1205, 1519
BstACI	GRCGYC	1 cut(s)	2880
BstBAI	YACGTR	2 cut(s)	1565, 2597
BstC8I	GCNNGC	4 cut(s)	768, 1747, 2811, 2925
BstDSI	CCRYGG	1 cut(s)	1041
BstENI	CCTNNNNNAGG	1 cut(s)	1125
BstF5I	GGATG	8 cut(s)	262, 283, 856, 989, 1600, 1712, 2287, 2787
BstHHI	GCGC	2 cut(s)	409, 2799
BstKTI	GATC	9 cut(s)	94, 355, 617, 1450, 2014, 2456, 2701, 2749, 2944
BstMAI	GTCTC	9 cut(s)	257, 597, 918, 1214, 2026, 2027, 2066, 2687, 2763
BstMBI	GATC	9 cut(s)	91, 352, 614, 1447, 2011, 2453, 2698, 2746, 2941
BstMWI	GCNNNNNNNGC	7 cut(s)	298, 442, 704, 2157, 2429, 2495, 2929
BstNI	CCWGG	5 cut(s)	144, 651, 776, 1687, 2766
BstNSI	RCATGY	3 cut(s)	659, 770, 2612
BstSCI	CCNGG	8 cut(s)	142, 649, 774, 1678, 1685, 1773, 2632, 2764
BstSFI	CTRYAG	5 cut(s)	440, 945, 1300, 1374, 2951
BstSLI	GKGCMC	1 cut(s)	1675
BstV1I	GCAGC	8 cut(s)	301, 712, 892, 959, 1360, 1388, 1827, 2212
BstV2I	GAAGAC	2 cut(s)	451, 2883
BstX2I	RGATCY	5 cut(s)	91, 614, 2011, 2453, 2698
BstXI	CCANNNNNNTGG	1 cut(s)	1048
BstYI	RGATCY	5 cut(s)	91, 614, 2011, 2453, 2698
Bsu36I	CCTNAGG	3 cut(s)	210, 1590, 1973
BsuI	GTATCC	1 cut(s)	637
BsuRI	GGCC	7 cut(s)	142, 390, 1040, 2250, 2266, 2660, 2756
BtgI	CCRYGG	1 cut(s)	1041
BtgZI	GCGATG	1 cut(s)	2924
BtrI	CACGTC	3 cut(s)	1048, 2483, 2603
BtsCI	GGATG	8 cut(s)	262, 283, 856, 989, 1600, 1712, 2287, 2787
BtsI	GCAGTG	2 cut(s)	1675, 2331
BtsIMutI	CAGTG	8 cut(s)	976, 1314, 1332, 1675, 2043, 2331, 2360, 2749
Cac8I	GCNNGC	4 cut(s)	768, 1747, 2811, 2925
CciI	TCATGA	2 cut(s)	1719, 1962
CfoI	GCGC	2 cut(s)	409, 2799
CseI	GACGC	1 cut(s)	493
Csp6I	GTAC	8 cut(s)	223, 975, 1486, 1562, 1568, 1948, 2637, 2707
CspCI	CAANNNNNGTGG	2 cut(s)	2283, 2318
CviQI	GTAC	8 cut(s)	223, 975, 1486, 1562, 1568, 1948, 2637, 2707
DpnI	GATC	9 cut(s)	93, 354, 616, 1449, 2013, 2455, 2700, 2748, 2943
DpnII	GATC	9 cut(s)	91, 352, 614, 1447, 2011, 2453, 2698, 2746, 2941
DrdI	GACNNNNNNGTC	1 cut(s)	545
DseDI	GACNNNNNNGTC	1 cut(s)	545
EaeI	YGGCCR	1 cut(s)	1038
Eam1104I	CTCTTC	2 cut(s)	1205, 1519
EarI	CTCTTC	2 cut(s)	1205, 1519
Ecl136II	GAGCTC	2 cut(s)	832, 1981
Eco130I	CCWWGG	4 cut(s)	267, 845, 1041, 2516
Eco147I	AGGCCT	1 cut(s)	2660
Eco24I	GRGCYC	6 cut(s)	834, 1170, 1780, 1983, 2391, 2811
Eco31I	GGTCTC	4 cut(s)	257, 1214, 2026, 2763
Eco47I	GGWCC	6 cut(s)	41, 778, 987, 1457, 1969, 2372
Eco53kI	GAGCTC	2 cut(s)	832, 1981
Eco57I	CTGAAG	2 cut(s)	750, 2179
Eco81I	CCTNAGG	3 cut(s)	210, 1590, 1973
Eco88I	CYCGRG	1 cut(s)	2832
EcoICRI	GAGCTC	2 cut(s)	832, 1981
EcoNI	CCTNNNNNAGG	1 cut(s)	1125
EcoO109I	RGGNCCY	3 cut(s)	140, 1969, 2372
EcoRI	GAATTC	1 cut(s)	2837
EcoRII	CCWGG	5 cut(s)	142, 649, 774, 1685, 2764
EcoT14I	CCWWGG	4 cut(s)	267, 845, 1041, 2516
EcoT22I	ATGCAT	1 cut(s)	1714
EcoT38I	GRGCYC	6 cut(s)	834, 1170, 1780, 1983, 2391, 2811
ErhI	CCWWGG	4 cut(s)	267, 845, 1041, 2516
FalI	AAGNNNNNCTT	2 cut(s)	838, 870
FaqI	GGGAC	2 cut(s)	2358, 2733
FauI	CCCGC	2 cut(s)	2818, 2861
FauNDI	CATATG	2 cut(s)	492, 525
FbaI	TGATCA	1 cut(s)	2746
FblI	GTMKAC	5 cut(s)	497, 1389, 1764, 2535, 2599
Fnu4HI	GCNGC	8 cut(s)	290, 726, 906, 948, 1374, 1377, 1841, 2226
FokI	GGATG	8 cut(s)	269, 290, 863, 996, 1607, 1699, 2294, 2774
FriOI	GRGCYC	6 cut(s)	834, 1170, 1780, 1983, 2391, 2811
Fsp4HI	GCNGC	8 cut(s)	290, 726, 906, 948, 1374, 1377, 1841, 2226
FspBI	CTAG	9 cut(s)	1101, 1380, 1503, 1571, 1647, 1833, 1931, 2777, 2828
GlaI	GCGC	2 cut(s)	408, 2798
GluI	GCNGC	8 cut(s)	290, 726, 906, 948, 1374, 1377, 1841, 2226
GsaI	CCCAGC	1 cut(s)	2932
GsuI	CTGGAG	4 cut(s)	942, 1107, 1184, 2625
HaeIII	GGCC	7 cut(s)	142, 390, 1040, 2250, 2266, 2660, 2756
HapII	CCGG	6 cut(s)	981, 1404, 1680, 1774, 2015, 2634
HgaI	GACGC	1 cut(s)	493
HhaI	GCGC	2 cut(s)	409, 2799
Hin1I	GRCGYC	1 cut(s)	2880
Hin6I	GCGC	2 cut(s)	407, 2797
HinP1I	GCGC	2 cut(s)	407, 2797
HincII	GTYRAC	4 cut(s)	372, 745, 814, 1390
HindII	GTYRAC	4 cut(s)	372, 745, 814, 1390
HindIII	AAGCTT	1 cut(s)	1025
HpaI	GTTAAC	1 cut(s)	745
HpaII	CCGG	6 cut(s)	981, 1404, 1680, 1774, 2015, 2634
HphI	GGTGA	6 cut(s)	263, 1211, 1353, 1578, 1952, 2198
Hpy99I	CGWCG	1 cut(s)	2056
HpyCH4III	ACNGT	6 cut(s)	378, 457, 611, 2038, 2567, 2587
HpyCH4IV	ACGT	9 cut(s)	362, 1047, 1275, 1564, 2230, 2482, 2596, 2602, 2880
HpyF10VI	GCNNNNNNNGC	7 cut(s)	298, 442, 704, 2157, 2429, 2495, 2929
HpySE526I	ACGT	9 cut(s)	362, 1047, 1275, 1564, 2230, 2482, 2596, 2602, 2880
Hsp92I	GRCGYC	1 cut(s)	2880
HspAI	GCGC	2 cut(s)	407, 2797
KpnI	GGTACC	1 cut(s)	2710
Ksp22I	TGATCA	1 cut(s)	2746
KspAI	GTTAAC	1 cut(s)	745
Kzo9I	GATC	9 cut(s)	91, 352, 614, 1447, 2011, 2453, 2698, 2746, 2941
LmnI	GCTCC	3 cut(s)	1165, 1978, 2818
Lsp1109I	GCAGC	8 cut(s)	301, 712, 892, 959, 1360, 1388, 1827, 2212
MaeI	CTAG	9 cut(s)	1101, 1380, 1503, 1571, 1647, 1833, 1931, 2777, 2828
MaeII	ACGT	9 cut(s)	362, 1047, 1275, 1564, 2230, 2482, 2596, 2602, 2880
MaeIII	GTNAC	8 cut(s)	29, 1243, 1474, 1621, 2186, 2569, 2592, 2611
MalI	GATC	9 cut(s)	93, 354, 616, 1449, 2013, 2455, 2700, 2748, 2943
MbiI	CCGCTC	1 cut(s)	2813
MboI	GATC	9 cut(s)	91, 352, 614, 1447, 2011, 2453, 2698, 2746, 2941
MfeI	CAATTG	1 cut(s)	876
MflI	RGATCY	5 cut(s)	91, 614, 2011, 2453, 2698
MhlI	GDGCHC	8 cut(s)	834, 1170, 1675, 1780, 1983, 2365, 2391, 2811
MlsI	TGGCCA	1 cut(s)	1040
MluCI	AATT	9 cut(s)	198, 661, 876, 1235, 1695, 1784, 1869, 2837, 2894
MluNI	TGGCCA	1 cut(s)	1040
MlyI	GAGTC	7 cut(s)	62, 555, 1240, 1726, 1919, 2327, 2687
MmeI	TCCRAC	3 cut(s)	19, 2318, 2495
Mox20I	TGGCCA	1 cut(s)	1040
Mph1103I	ATGCAT	1 cut(s)	1714
MscI	TGGCCA	1 cut(s)	1040
MseI	TTAA	7 cut(s)	504, 689, 744, 888, 1470, 1694, 1788
MslI	CAYNNNNRTG	4 cut(s)	467, 628, 1046, 1337
Msp20I	TGGCCA	1 cut(s)	1040
MspA1I	CMGCKG	2 cut(s)	950, 2886
MspI	CCGG	6 cut(s)	981, 1404, 1680, 1774, 2015, 2634
MspR9I	CCNGG	8 cut(s)	144, 651, 776, 1680, 1687, 1775, 2634, 2766
MunI	CAATTG	1 cut(s)	876
MvaI	CCWGG	5 cut(s)	144, 651, 776, 1687, 2766
MwoI	GCNNNNNNNGC	7 cut(s)	298, 442, 704, 2157, 2429, 2495, 2929
NciI	CCSGG	3 cut(s)	1680, 1775, 2634
NcoI	CCATGG	1 cut(s)	1041
NdeI	CATATG	2 cut(s)	492, 525
NdeII	GATC	9 cut(s)	91, 352, 614, 1447, 2011, 2453, 2698, 2746, 2941
NmuCI	GTSAC	6 cut(s)	1243, 1474, 1621, 2186, 2592, 2611
NsiI	ATGCAT	1 cut(s)	1714
NspI	RCATGY	3 cut(s)	659, 770, 2612
PaeI	GCATGC	1 cut(s)	770
PagI	TCATGA	2 cut(s)	1719, 1962
PceI	AGGCCT	1 cut(s)	2660
PciI	ACATGT	1 cut(s)	655
PfeI	GAWTC	6 cut(s)	1415, 1430, 1828, 1955, 2045, 2467
Pfl23II	CGTACG	1 cut(s)	1561
PflMI	CCANNNNNTGG	1 cut(s)	1130
PfoI	TCCNGGA	1 cut(s)	774
PkrI	GCNGC	8 cut(s)	291, 727, 907, 949, 1375, 1378, 1842, 2227
PleI	GAGTC	7 cut(s)	61, 554, 1240, 1726, 1918, 2327, 2686
PpsI	GAGTC	7 cut(s)	61, 554, 1240, 1726, 1918, 2327, 2686
Ppu21I	YACGTR	2 cut(s)	1565, 2597
PpuMI	RGGWCCY	2 cut(s)	1969, 2372
PscI	ACATGT	1 cut(s)	655
PshBI	ATTAAT	1 cut(s)	888
Psp124BI	GAGCTC	2 cut(s)	834, 1983
Psp5II	RGGWCCY	2 cut(s)	1969, 2372
Psp6I	CCWGG	5 cut(s)	142, 649, 774, 1685, 2764
PspFI	CCCAGC	1 cut(s)	2928
PspGI	CCWGG	5 cut(s)	142, 649, 774, 1685, 2764
PspLI	CGTACG	1 cut(s)	1561
PspPPI	RGGWCCY	2 cut(s)	1969, 2372
PstI	CTGCAG	3 cut(s)	949, 1378, 2955
PsuI	RGATCY	5 cut(s)	91, 614, 2011, 2453, 2698
PvuII	CAGCTG	1 cut(s)	950
RsaI	GTAC	8 cut(s)	224, 976, 1487, 1563, 1569, 1949, 2638, 2708
RsaNI	GTAC	8 cut(s)	223, 975, 1486, 1562, 1568, 1948, 2637, 2707
RseI	CAYNNNNRTG	4 cut(s)	467, 628, 1046, 1337
SacI	GAGCTC	2 cut(s)	834, 1983
SalI	GTCGAC	1 cut(s)	1388
SaqAI	TTAA	7 cut(s)	504, 689, 744, 888, 1470, 1694, 1788
SatI	GCNGC	8 cut(s)	290, 726, 906, 948, 1374, 1377, 1841, 2226
Sau3AI	GATC	9 cut(s)	91, 352, 614, 1447, 2011, 2453, 2698, 2746, 2941
ScaI	AGTACT	1 cut(s)	224
SchI	GAGTC	7 cut(s)	62, 555, 1240, 1726, 1919, 2327, 2687
ScrFI	CCNGG	8 cut(s)	144, 651, 776, 1680, 1687, 1775, 2634, 2766
SduI	GDGCHC	8 cut(s)	834, 1170, 1675, 1780, 1983, 2365, 2391, 2811
SfcI	CTRYAG	5 cut(s)	440, 945, 1300, 1374, 2951
SinI	GGWCC	6 cut(s)	41, 778, 987, 1457, 1969, 2372
SmiMI	CAYNNNNRTG	4 cut(s)	467, 628, 1046, 1337
SmlI	CTYRAG	3 cut(s)	296, 602, 2804
SmoI	CTYRAG	3 cut(s)	296, 602, 2804
SpeI	ACTAGT	1 cut(s)	1502
SphI	GCATGC	1 cut(s)	770
Sse9I	AATT	9 cut(s)	198, 661, 876, 1235, 1695, 1784, 1869, 2837, 2894
SseBI	AGGCCT	1 cut(s)	2660
SsiI	CCGC	4 cut(s)	581, 2811, 2854, 2886
SspMI	CTAG	9 cut(s)	1101, 1380, 1503, 1571, 1647, 1833, 1931, 2777, 2828
SstI	GAGCTC	2 cut(s)	834, 1983
StuI	AGGCCT	1 cut(s)	2660
StyD4I	CCNGG	8 cut(s)	142, 649, 774, 1678, 1685, 1773, 2632, 2764
StyI	CCWWGG	4 cut(s)	267, 845, 1041, 2516
TaaI	ACNGT	6 cut(s)	378, 457, 611, 2038, 2567, 2587
TaiI	ACGT	9 cut(s)	365, 1050, 1278, 1567, 2233, 2485, 2599, 2605, 2883
TaqI	TCGA	7 cut(s)	549, 1066, 1389, 1707, 1995, 2054, 2562
TasI	AATT	9 cut(s)	198, 661, 876, 1235, 1695, 1784, 1869, 2837, 2894
TatI	WGTACW	3 cut(s)	222, 1567, 1947
TfiI	GAWTC	6 cut(s)	1415, 1430, 1828, 1955, 2045, 2467
Tru1I	TTAA	7 cut(s)	504, 689, 744, 888, 1470, 1694, 1788
Tru9I	TTAA	7 cut(s)	504, 689, 744, 888, 1470, 1694, 1788
TscAI	CASTG	8 cut(s)	976, 1314, 1339, 1675, 2043, 2331, 2360, 2756
TseFI	GTSAC	6 cut(s)	1243, 1474, 1621, 2186, 2592, 2611
TseI	GCWGC	8 cut(s)	289, 725, 905, 947, 1373, 1376, 1840, 2225
Tsp45I	GTSAC	6 cut(s)	1243, 1474, 1621, 2186, 2592, 2611
TspGWI	ACGGA	1 cut(s)	2440
TspRI	CASTG	8 cut(s)	976, 1314, 1339, 1675, 2043, 2331, 2360, 2756
Van91I	CCANNNNNTGG	1 cut(s)	1130
VpaK11BI	GGWCC	6 cut(s)	41, 778, 987, 1457, 1969, 2372
VspI	ATTAAT	1 cut(s)	888
XagI	CCTNNNNNAGG	1 cut(s)	1125
XapI	RAATTY	2 cut(s)	2837, 2894
XbaI	TCTAGA	1 cut(s)	2776
XceI	RCATGY	3 cut(s)	659, 770, 2612
XmiI	GTMKAC	5 cut(s)	497, 1389, 1764, 2535, 2599
XspI	CTAG	9 cut(s)	1101, 1380, 1503, 1571, 1647, 1833, 1931, 2777, 2828
ZraI	GACGTC	1 cut(s)	2881
ZrmI	AGTACT	1 cut(s)	224
Zsp2I	ATGCAT	1 cut(s)	1714

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.