Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0002643

Physical Location & Seq

Genomic Coordinates Forward (+)

21855611 .. 21861354

Gene Structure 5744 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00399840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2871 bp

ATGGATGATAGTCTATACGACGAGTTCGGGAACTATATAGGACCCGAAATCGAGTCGGACCAGGAGAGTGATAGGGAGGAAGAAGATGAGGAGCTTCCGGATAGGTTAGAAGATAGAGGTGCGGCATCCGACGGTGAGGATGCTCCGAATGGGTGGATGACTGCCTCTAATGATGTAGATATGGACAACCAGGTTGTTCTTGCTGAGGATAAGAAGTATTATCCTACAGCGGAGGAGGTGTACGGTGAAGATGTTGAGACATTGGTCATGGATGAAGATGCACAGCCCCTTGAACAGCCGATTATCAAGGCGGTTCGGAATATTAAGTTTGAGGTTGGTGTCAAGGACTCCTCGACGTATGTGTCGACGCAGTTTCTTGTTGGTCTTATGTCGAACCCTGCTCTGGTTCGGAATGTGGCGCTGGTGGGGCACTTGCAGCATGGGAAGACGGTGTTTATGGATATGTTGGTGGAACAGACTCATCATATGTCTACTTTTGATGCAAATAGTGACAAGCACATGAGGTACACTGATACGAGGATTGATGAACAAGAGAGGAGGATATCTATTAAGGCTGTTCCCATGTCCCTTGTACTTGAGGATAGCAATTCAAAGTCGTACCTGTGCAATGTCATGGATACTCCTGGTCATGTCAATTTCTCTGATGAAATGACTGCTTCACTTAGGCTCGCTGATGGTGCTGTCCTCATTGTAAATACTGAGAGGGCTATACGCCATGCAATCCAAGAGCGCCTACCAATTGTGGTTGTAATCAACAAGGTTGACAGGTTGATAACAGAACTTAAATTGCCCCCAAGGGATGCTTACTTCAAGCTTAGGCATACTCTGGAAGTGATTAATAACCACATAACGGCTGCCTCTTCAACTGCTGGTCATGTACAAGTTATTGATCCTGTTGCTGGAAATGTATGCTTTGCAAGCGCTACTGCAGGATGGTCGTTCACGTTGCAATCATTTGCGAAGCTATATGTTAAGCTTCATGGGATCGCTTTTGACGCGGATCAGTTTGCTTCTCGTCTATGGGGAGATATTTACTATCATCCTGAGGACAGGGCTTTCAGGAGAAACCCCCTGCAAGTGATTGGAGAACATAAAAAGAGTGTTGAGGCAACTCTTGCTGAACTTGGTGTCACCCTTAGCAATGCAGCTTACAAATTGAATGTTAGGCCTCTACTGAGGTTGGCTTGTAGCTCGGTTTTTGGATCTGCCTCGGGCTTCACTGATATGCTTGTGCAACATATTCCTTCTCCTAAAGATGCTGCAACAAGGAAAGTCGATCACACATATACTGGACCAAAAGACTCTTTGATTTACAAAGCCATGAAAAGTTGTGATCCCAATGGCCCGTTGATGGTAAATGTTACCAAGCTGTATCCCAAGTCTGATTGTAGCGTGTTCGATGCATTTGGTAGGGTATACAGTGGCAAGATTCAGACAGGACAGTCAGTACGAGTATTGGGAGAAGGGTATTCACCTGACGATGAGGAGGACATGACAGTCAAAGAAGTAACGAAGCTATGGCTTTACCAAGCACGAGATAGGATACCTATAGCTGAAGCTCCTCCTGGTACTTGGGTTCTCATCGAAGGTGTGGATGCTTCCATAATGAAAACTGCCACTCTGTGTAATGAGAGTTATGACGAGGATGTATACATATTCCGTCCTCTTCAGTTCAATACACTGCCAGTGGTGAAAACGGCAACTGAGCCTCTAAATCCCAGTGAGTTGCCAAAAATGGTGGAGGGTCTTAGAAAGATCAGCAAGAGTTATCCTCTTGCCATCACCAAAGTTGAGGAGTCTGGAGAGCACACAATCTTAGGTACAGGAGAACTGTACTTGGATTCCATCATGAAGGACCTCAGAGAGCTCTACTCCGAAGTAGAAGTCAAGGTTGCAGATCCTGTTGTTTCATTCTGCGAAACAGTTGTGGAATCTTCATCAATGAAGTGTTTTGCTGAAACACCAAACAAGAAGAATAAAATTACTATGATTGCAGAGCCATTAGAGAGAGGTCTTGCAGAGGACATTGAGAAAGGTGTTGTAAGCATTGATTGGTCTAGGAGAGATATAGGTAATTTCTTCCAGACTAGGTATGAGTGGGATGTTCTTGCTGCACGGTCCATATGGGCATTCGGTCCTGATAAGCAGGGACCCAACATACTACTTGATGACACACTTTCAAGCGAAGTTGACAAGAGCTTGCTGAATGCTGTGAAAGACTCCATTGTGCAAGGGTTTCAGTGGGGTGCTCGAGAAGGACCTCTGTGTGATGAACCCATCAGAAATGTTAAGTTCAAGATAGTTGATGCAAGGATTGCACCTGAGCCGTTGCATCGTGGATCTGGTCAAATCATTCCCACATCTCGACGTGTGGCCTATTCATCTTTCCTAATGGCAACCCCACGGCTCATGGAGCCAGTATATTATGTGGAGATACAAACGCCAATCGACTGTATATCTGCAATCTACACTGTTTTATCTCGCAGGCGTGGACATGTTACAGCCGATGTTCCTCAACCCGGGACCCCAGCTTACATCGTTAAGGCATTTTTACCCGTCATAGAATCGTTTGGTTTTGAGACGGACTTGAGGTACCATACTCAAGGACAGGCTTTTTGCCTCTCAGTCTTTGATCATTGGGCTATAGTTCCTGGGGATCCTCTTGACAAGAGTATTGTTCTGCGACCACTTGAACCTGCACCGATTCAGCACCTGGCTCGTGAATTTATGGTCAAGACAAGGCGTAGAAAGGGAATGAGCGAGGATGTGAGCATCAGCAAATTTTTCGATGAGGCCATGATGGTGGAACTGGCTCAGCAGGAAGCTGATATTCACCAGCAAATGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005730 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0007275 GO:0008150 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0010154 GO:0015030 GO:0016020 GO:0016604 GO:0016607 GO:0022414 GO:0030054 GO:0030312 GO:0030532 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043900 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045694 GO:0046540 GO:0048316 GO:0048367 GO:0048437 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0070013 GO:0071007 GO:0071013 GO:0071944 GO:0090567 GO:0097525 GO:0097526 GO:0099402 GO:0120114 GO:1902494 GO:1990904 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

EFG_C EFG_IV EFTUD2 GTP_EFTU GTP_EFTU_D2

Protein Analysis

956

Amino Acids

107.08

Weight (kDa)

5.02

Isoelectric Point (pI)

40.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EFTUD2	PF16004	3 - 116	1.2e-34	116 kDa U5 small nuclear ribonucleoprotein component N-terminus
GTP_EFTU	PF00009	136 - 322	2.3e-33	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	475 - 549	2.7e-10	Elongation factor Tu domain 2
EFG_IV	PF03764	695 - 806	1.1e-18	Elongation factor G, domain IV
EFG_C	PF00679	809 - 897	3.5e-22	Elongation factor G C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002643)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06220 AT1G06220 AT1G06220 AT5G25230 AT5G25230
fragaria_vesca	FvH4_2g24581 FvH4_7g03790 FvH4_7g03790 FvH4_7g03790
malus_domestica	MD02G1013600.v1.1 MD15G1160400.v1.1
prunus_persica	Prupe.3G036300_v2.0.a1
pyrus_communis	pycom02g01260 pycom15g14420
rosa_chinensis	RchiOBHm_Chr1g0327101 RchiOBHm_Chr1g0327121 RchiOBHm_Chr2g0127751
rosa_laevigata	RLG00000012029 RLG00000012030 RLG00000030043
rosa_multiflora	Rmu_sc0000795.1_g000050 Rmu_sc0004240.1_g000007
rosa_roxburghii	Rroxscaffold_4G00323330 Rroxscaffold_6G00399840 Rroxscaffold_6G00400180
rosa_rugosa	Rorug01G0063500
rosa_samantha	Rh1AG080100 Rh1AG080300 Rh1AG080700 Rh1AG080900 Rh1BG064100 Rh1CG078700 Rh1CG078800 Rh1DG085100 Rh1DG085200 Rh2AG323400 Rh2BG332600 Rh2DG350900
rosa_wichuraiana	Rw1G006400 Rw2G026250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	361, 1462, 1517
Acc36I	ACCTGC	1 cut(s)	2725
Acc65I	GGTACC	1 cut(s)	2613
AccB1I	GGYRCC	1 cut(s)	2613
AccI	GTMKAC	4 cut(s)	365, 491, 1437, 1671
AccII	CGCG	1 cut(s)	1019
AccIII	TCCGGA	1 cut(s)	97
AciI	CCGC	4 cut(s)	122, 230, 311, 1019
AclWI	GGATC	9 cut(s)	905, 1013, 1029, 1231, 1349, 1913, 2368, 2672, 2685
AcsI	RAATTY	2 cut(s)	2744, 2801
AcuI	CTGAAG	2 cut(s)	1596, 1673
AdeI	CACNNNGTG	1 cut(s)	1644
AfeI	AGCGCT	1 cut(s)	943
AfiI	CCNNNNNNNGG	5 cut(s)	403, 871, 920, 1372, 2540
AflIII	ACRYGT	2 cut(s)	2389, 2515
AgsI	TTSAA	9 cut(s)	293, 612, 832, 885, 1180, 1696, 2202, 2317, 2714
AjiI	CACGTC	1 cut(s)	2390
AjnI	CCWGG	6 cut(s)	60, 189, 643, 1585, 2671, 2733
Alw21I	GWGCWC	3 cut(s)	1830, 1890, 2272
Alw26I	GTCTC	2 cut(s)	251, 2594
AlwI	GGATC	9 cut(s)	905, 1013, 1029, 1231, 1349, 1913, 2368, 2672, 2685
AlwNI	CAGNNNCTG	2 cut(s)	1922, 2734
Ama87I	CYCGRG	3 cut(s)	1231, 2271, 2540
Aor13HI	TCCGGA	1 cut(s)	97
Aor51HI	AGCGCT	1 cut(s)	943
AoxI	GGCC	4 cut(s)	1187, 1363, 2394, 2814
ApeKI	GCWGC	5 cut(s)	436, 875, 1166, 1280, 2132
ApoI	RAATTY	2 cut(s)	2744, 2801
ArsI	GACNNNNNNTTYG	4 cut(s)	1890, 1922, 2619, 2651
AseI	ATTAAT	1 cut(s)	858
Asp718I	GGTACC	1 cut(s)	2613
AspLEI	GCGC	3 cut(s)	421, 753, 944
AsuC2I	CCSGG	2 cut(s)	2541, 2542
AsuHPI	GGTGA	7 cut(s)	146, 257, 1144, 1485, 1723, 1795, 2846
AvaI	CYCGRG	3 cut(s)	1231, 2271, 2540
AvaII	GGWCC	9 cut(s)	41, 58, 1313, 1876, 2139, 2156, 2171, 2279, 2544
AxyI	CCTNAGG	1 cut(s)	1065
BaeGI	GKGCMC	1 cut(s)	432
BaeI	ACNNNNGTAYC	2 cut(s)	1581, 1614
BamHI	GGATCC	1 cut(s)	2677
BanI	GGYRCC	1 cut(s)	2613
BanII	GRGCYC	1 cut(s)	1890
BauI	CACGAG	2 cut(s)	1554, 2739
BbsI	GAAGAC	1 cut(s)	452
Bbv12I	GWGCWC	3 cut(s)	1830, 1890, 2272
BbvCI	CCTCAGC	1 cut(s)	204
BbvI	GCAGC	5 cut(s)	448, 862, 1178, 1267, 2119
BccI	CCATC	7 cut(s)	689, 948, 1366, 1808, 1874, 2306, 2815
BceAI	ACGGC	4 cut(s)	888, 1734, 2332, 2441
BcgI	CGANNNNNNTGC	6 cut(s)	939, 973, 2720, 2754, 2788, 2822
BciT130I	CCWGG	6 cut(s)	62, 191, 645, 1587, 2673, 2735
BciVI	GTATCC	3 cut(s)	631, 1404, 1557
BclI	TGATCA	1 cut(s)	2653
BcnI	CCSGG	2 cut(s)	2541, 2542
BcoDI	GTCTC	2 cut(s)	251, 2594
BfaI	CTAG	2 cut(s)	2079, 2109
BfmI	CTRYAG	4 cut(s)	225, 948, 1569, 2664
BfoI	RGCGCY	3 cut(s)	422, 754, 945
BfuAI	ACCTGC	1 cut(s)	2725
BfuI	GTATCC	3 cut(s)	631, 1404, 1557
BisI	GCNGC	6 cut(s)	123, 437, 876, 1167, 1281, 2133
BlpI	GCTNAGC	1 cut(s)	2835
BlsI	GCNGC	6 cut(s)	124, 438, 877, 1168, 1282, 2134
Bme1390I	CCNGG	8 cut(s)	62, 191, 645, 1587, 2541, 2542, 2673, 2735
Bme18I	GGWCC	9 cut(s)	41, 58, 1313, 1876, 2139, 2156, 2171, 2279, 2544
BmeT110I	CYCGRG	3 cut(s)	1231, 2271, 2540
BmgBI	CACGTC	1 cut(s)	2390
BmiI	GGNNCC	8 cut(s)	43, 2172, 2173, 2436, 2545, 2546, 2615, 2679
BmrFI	CCNGG	8 cut(s)	62, 191, 645, 1587, 2541, 2542, 2673, 2735
BmrI	ACTGGG	1 cut(s)	1734
BmuI	ACTGGG	1 cut(s)	1734
BoxI	GACNNNNGTC	1 cut(s)	263
BpiI	GAAGAC	1 cut(s)	452
BplI	GAGNNNNNCTC	4 cut(s)	1777, 1809, 1877, 1909
BpmI	CTGGAG	1 cut(s)	1842
Bpu10I	CCTNAGC	4 cut(s)	204, 836, 1157, 2343
Bpu1102I	GCTNAGC	1 cut(s)	2835
BpuEI	CTTGAG	3 cut(s)	617, 2607, 2629
BpuMI	CCSGG	2 cut(s)	2541, 2542
BsaJI	CCNNGG	5 cut(s)	815, 1230, 2423, 2540, 2672
BsaWI	WCCGGW	1 cut(s)	97
BsaXI	ACNNNNNCTCC	2 cut(s)	224, 254
Bsc4I	CCNNNNNNNGG	5 cut(s)	403, 871, 920, 1372, 2540
Bse1I	ACTGG	5 cut(s)	1315, 1706, 1740, 2438, 2835
Bse21I	CCTNAGG	1 cut(s)	1065
Bse3DI	GCAATG	2 cut(s)	634, 1168
BseAI	TCCGGA	1 cut(s)	97
BseBI	CCWGG	6 cut(s)	62, 191, 645, 1587, 2673, 2735
BseDI	CCNNGG	5 cut(s)	815, 1230, 2423, 2540, 2672
BseLI	CCNNNNNNNGG	5 cut(s)	403, 871, 920, 1372, 2540
BseMI	GCAATG	2 cut(s)	634, 1168
BseMII	CTCAG	9 cut(s)	195, 711, 1056, 1187, 1716, 1894, 2334, 2658, 2849
BseNI	ACTGG	5 cut(s)	1315, 1706, 1740, 2438, 2835
BseRI	GAGGAG	7 cut(s)	104, 248, 340, 571, 1520, 1572, 1829
BseSI	GKGCMC	1 cut(s)	432
BseXI	GCAGC	5 cut(s)	448, 862, 1178, 1267, 2119
BseYI	CCCAGC	1 cut(s)	2548
BsgI	GTGCAG	2 cut(s)	2118, 2703
Bsh1236I	CGCG	1 cut(s)	1019
BshFI	GGCC	4 cut(s)	1189, 1365, 2396, 2816
BshNI	GGYRCC	1 cut(s)	2613
BsiHKAI	GWGCWC	3 cut(s)	1830, 1890, 2272
BsiHKCI	CYCGRG	3 cut(s)	1231, 2271, 2540
BsiSI	CCGG	2 cut(s)	98, 2541
BslFI	GGGAC	3 cut(s)	571, 2184, 2557
BslI	CCNNNNNNNGG	5 cut(s)	403, 871, 920, 1372, 2540
BsmAI	GTCTC	2 cut(s)	251, 2594
BsmBI	CGTCTC	1 cut(s)	2594
BsmFI	GGGAC	3 cut(s)	571, 2184, 2557
BsmI	GAATGC	2 cut(s)	2150, 2233
BsnI	GGCC	4 cut(s)	1189, 1365, 2396, 2816
BsoBI	CYCGRG	3 cut(s)	1231, 2271, 2540
Bsp1286I	GDGCHC	4 cut(s)	432, 1830, 1890, 2272
Bsp13I	TCCGGA	1 cut(s)	97
Bsp1407I	TGTACA	1 cut(s)	898
Bsp1720I	GCTNAGC	1 cut(s)	2835
BspACI	CCGC	4 cut(s)	122, 230, 311, 1019
BspANI	GGCC	4 cut(s)	1189, 1365, 2396, 2816
BspCNI	CTCAG	9 cut(s)	196, 712, 1057, 1188, 1717, 1893, 2335, 2657, 2848
BspEI	TCCGGA	1 cut(s)	97
BspFNI	CGCG	1 cut(s)	1019
BspHI	TCATGA	1 cut(s)	1869
BspLI	GGNNCC	8 cut(s)	43, 2172, 2173, 2436, 2545, 2546, 2615, 2679
BspMAI	CTGCAG	1 cut(s)	952
BspMI	ACCTGC	1 cut(s)	2725
BspPI	GGATC	9 cut(s)	905, 1013, 1029, 1231, 1349, 1913, 2368, 2672, 2685
BspT107I	GGYRCC	1 cut(s)	2613
BsrDI	GCAATG	2 cut(s)	634, 1168
BsrGI	TGTACA	1 cut(s)	898
BsrI	ACTGG	5 cut(s)	1315, 1706, 1740, 2438, 2835
BssECI	CCNNGG	5 cut(s)	815, 1230, 2423, 2540, 2672
BssNAI	GTATAC	2 cut(s)	1438, 1672
BssSI	CACGAG	2 cut(s)	1554, 2739
BssT1I	CCWWGG	1 cut(s)	815
Bst1107I	GTATAC	2 cut(s)	1438, 1672
Bst2BI	CACGAG	2 cut(s)	1554, 2739
Bst2UI	CCWGG	6 cut(s)	62, 191, 645, 1587, 2673, 2735
Bst6I	CTCTTC	2 cut(s)	886, 1692
BstAPI	GCANNNNNTGC	2 cut(s)	1136, 2336
BstAUI	TGTACA	1 cut(s)	898
BstC8I	GCNNGC	4 cut(s)	690, 940, 2222, 2507
BstDSI	CCRYGG	1 cut(s)	2423
BstFNI	CGCG	1 cut(s)	1019
BstH2I	RGCGCY	3 cut(s)	422, 754, 945
BstHHI	GCGC	3 cut(s)	421, 753, 944
BstMAI	GTCTC	2 cut(s)	251, 2594
BstMWI	GCNNNNNNNGC	8 cut(s)	427, 436, 698, 939, 1016, 1136, 2336, 2434
BstNI	CCWGG	6 cut(s)	62, 191, 645, 1587, 2673, 2735
BstNSI	RCATGY	1 cut(s)	2519
BstPAI	GACNNNNGTC	1 cut(s)	263
BstSCI	CCNGG	8 cut(s)	60, 189, 643, 1585, 2539, 2540, 2671, 2733
BstSFI	CTRYAG	4 cut(s)	225, 948, 1569, 2664
BstSLI	GKGCMC	1 cut(s)	432
BstUI	CGCG	1 cut(s)	1019
BstV1I	GCAGC	5 cut(s)	448, 862, 1178, 1267, 2119
BstV2I	GAAGAC	1 cut(s)	452
BstX2I	RGATCY	4 cut(s)	1223, 1918, 2360, 2677
BstXI	CCANNNNNNTGG	1 cut(s)	2824
BstYI	RGATCY	4 cut(s)	1223, 1918, 2360, 2677
BstZ17I	GTATAC	2 cut(s)	1438, 1672
Bsu36I	CCTNAGG	1 cut(s)	1065
BsuI	GTATCC	3 cut(s)	631, 1404, 1557
BsuRI	GGCC	4 cut(s)	1189, 1365, 2396, 2816
BtgI	CCRYGG	1 cut(s)	2423
BtrI	CACGTC	1 cut(s)	2390
BtsI	GCAGTG	1 cut(s)	1700
BtsIMutI	CAGTG	8 cut(s)	528, 1239, 1447, 1700, 1713, 1747, 2267, 2490
BveI	ACCTGC	1 cut(s)	2725
Cac8I	GCNNGC	4 cut(s)	690, 940, 2222, 2507
CaiI	CAGNNNCTG	2 cut(s)	1922, 2734
CciI	TCATGA	1 cut(s)	1869
CfoI	GCGC	3 cut(s)	421, 753, 944
Cfr9I	CCCGGG	1 cut(s)	2540
CseI	GACGC	2 cut(s)	376, 1025
CsiI	ACCWGGT	1 cut(s)	189
CspCI	CAANNNNNGTGG	2 cut(s)	1740, 1775
DraIII	CACNNNGTG	1 cut(s)	1644
DrdI	GACNNNNNNGTC	3 cut(s)	361, 1462, 1517
DseDI	GACNNNNNNGTC	3 cut(s)	361, 1462, 1517
Eam1104I	CTCTTC	2 cut(s)	886, 1692
EarI	CTCTTC	2 cut(s)	886, 1692
Ecl136II	GAGCTC	1 cut(s)	1888
Eco130I	CCWWGG	1 cut(s)	815
Eco147I	AGGCCT	1 cut(s)	1189
Eco24I	GRGCYC	1 cut(s)	1890
Eco32I	GATATC	1 cut(s)	564
Eco47I	GGWCC	9 cut(s)	41, 58, 1313, 1876, 2139, 2156, 2171, 2279, 2544
Eco47III	AGCGCT	1 cut(s)	943
Eco53kI	GAGCTC	1 cut(s)	1888
Eco57I	CTGAAG	2 cut(s)	1596, 1673
Eco81I	CCTNAGG	1 cut(s)	1065
Eco88I	CYCGRG	3 cut(s)	1231, 2271, 2540
EcoICRI	GAGCTC	1 cut(s)	1888
EcoO109I	RGGNCCY	5 cut(s)	41, 1876, 2171, 2279, 2544
EcoRII	CCWGG	6 cut(s)	60, 189, 643, 1585, 2671, 2733
EcoRV	GATATC	1 cut(s)	564
EcoT14I	CCWWGG	1 cut(s)	815
EcoT22I	ATGCAT	1 cut(s)	1426
EcoT38I	GRGCYC	1 cut(s)	1890
ErhI	CCWWGG	1 cut(s)	815
Esp3I	CGTCTC	1 cut(s)	2594
FalI	AAGNNNNNCTT	2 cut(s)	808, 840
FaqI	GGGAC	3 cut(s)	571, 2184, 2557
FauNDI	CATATG	2 cut(s)	486, 2144
FbaI	TGATCA	1 cut(s)	2653
FblI	GTMKAC	4 cut(s)	365, 491, 1437, 1671
Fnu4HI	GCNGC	6 cut(s)	123, 437, 876, 1167, 1281, 2133
FriOI	GRGCYC	1 cut(s)	1890
Fsp4HI	GCNGC	6 cut(s)	123, 437, 876, 1167, 1281, 2133
FspBI	CTAG	2 cut(s)	2079, 2109
GlaI	GCGC	3 cut(s)	420, 752, 943
GluI	GCNGC	6 cut(s)	123, 437, 876, 1167, 1281, 2133
GsaI	CCCAGC	1 cut(s)	2552
GsuI	CTGGAG	1 cut(s)	1842
HaeII	RGCGCY	3 cut(s)	422, 754, 945
HaeIII	GGCC	4 cut(s)	1189, 1365, 2396, 2816
HapII	CCGG	2 cut(s)	98, 2541
HgaI	GACGC	2 cut(s)	376, 1025
HhaI	GCGC	3 cut(s)	421, 753, 944
Hin6I	GCGC	3 cut(s)	419, 751, 942
HinP1I	GCGC	3 cut(s)	419, 751, 942
HincII	GTYRAC	3 cut(s)	366, 784, 2212
HindII	GTYRAC	3 cut(s)	366, 784, 2212
HindIII	AAGCTT	2 cut(s)	833, 995
HpaII	CCGG	2 cut(s)	98, 2541
HphI	GGTGA	7 cut(s)	146, 257, 1144, 1485, 1723, 1795, 2846
Hpy99I	CGWCG	5 cut(s)	23, 134, 358, 370, 2391
HpyAV	CCTTC	5 cut(s)	1275, 1478, 1601, 1867, 2270
HpyCH4IV	ACGT	3 cut(s)	356, 965, 2389
HpyF10VI	GCNNNNNNNGC	8 cut(s)	427, 436, 698, 939, 1016, 1136, 2336, 2434
HpySE526I	ACGT	3 cut(s)	356, 965, 2389
HspAI	GCGC	3 cut(s)	419, 751, 942
KflI	GGGWCCC	2 cut(s)	2171, 2544
Kpn2I	TCCGGA	1 cut(s)	97
KpnI	GGTACC	1 cut(s)	2617
Ksp22I	TGATCA	1 cut(s)	2653
LmnI	GCTCC	4 cut(s)	91, 148, 1585, 2434
Lsp1109I	GCAGC	5 cut(s)	448, 862, 1178, 1267, 2119
MabI	ACCWGGT	1 cut(s)	189
MaeI	CTAG	2 cut(s)	2079, 2109
MaeII	ACGT	3 cut(s)	356, 965, 2389
MaeIII	GTNAC	5 cut(s)	509, 1150, 1381, 1528, 2518
MfeI	CAATTG	1 cut(s)	759
MflI	RGATCY	4 cut(s)	1223, 1918, 2360, 2677
MhlI	GDGCHC	4 cut(s)	432, 1830, 1890, 2272
MluCI	AATT	9 cut(s)	607, 655, 759, 806, 1175, 2001, 2095, 2744, 2801
MlyI	GAGTC	6 cut(s)	62, 341, 472, 1316, 1826, 2234
MmeI	TCCRAC	2 cut(s)	36, 153
Mph1103I	ATGCAT	1 cut(s)	1426
MroI	TCCGGA	1 cut(s)	97
MseI	TTAA	7 cut(s)	324, 570, 804, 858, 993, 2310, 2562
MslI	CAYNNNNRTG	2 cut(s)	1244, 2822
MspA1I	CMGCKG	1 cut(s)	230
MspI	CCGG	2 cut(s)	98, 2541
MspR9I	CCNGG	8 cut(s)	62, 191, 645, 1587, 2541, 2542, 2673, 2735
MunI	CAATTG	1 cut(s)	759
Mva1269I	GAATGC	2 cut(s)	2150, 2233
MvaI	CCWGG	6 cut(s)	62, 191, 645, 1587, 2673, 2735
MvnI	CGCG	1 cut(s)	1019
MwoI	GCNNNNNNNGC	8 cut(s)	427, 436, 698, 939, 1016, 1136, 2336, 2434
NciI	CCSGG	2 cut(s)	2541, 2542
NdeI	CATATG	2 cut(s)	486, 2144
NlaIV	GGNNCC	8 cut(s)	43, 2172, 2173, 2436, 2545, 2546, 2615, 2679
NmuCI	GTSAC	2 cut(s)	509, 1150
NsiI	ATGCAT	1 cut(s)	1426
NspI	RCATGY	1 cut(s)	2519
PaeR7I	CTCGAG	1 cut(s)	2271
PagI	TCATGA	1 cut(s)	1869
PceI	AGGCCT	1 cut(s)	1189
PciI	ACATGT	1 cut(s)	2515
PcsI	WCGNNNNNNNCGW	1 cut(s)	362
PctI	GAATGC	2 cut(s)	2150, 2233
PfeI	GAWTC	5 cut(s)	1450, 1862, 1952, 2585, 2725
PkrI	GCNGC	6 cut(s)	124, 438, 877, 1168, 1282, 2134
PleI	GAGTC	6 cut(s)	61, 341, 472, 1316, 1825, 2234
PpsI	GAGTC	6 cut(s)	61, 341, 472, 1316, 1825, 2234
PpuMI	RGGWCCY	5 cut(s)	41, 1876, 2171, 2279, 2544
PscI	ACATGT	1 cut(s)	2515
PshAI	GACNNNNGTC	1 cut(s)	263
PshBI	ATTAAT	1 cut(s)	858
Psp124BI	GAGCTC	1 cut(s)	1890
Psp5II	RGGWCCY	5 cut(s)	41, 1876, 2171, 2279, 2544
Psp6I	CCWGG	6 cut(s)	60, 189, 643, 1585, 2671, 2733
PspFI	CCCAGC	1 cut(s)	2548
PspGI	CCWGG	6 cut(s)	60, 189, 643, 1585, 2671, 2733
PspN4I	GGNNCC	8 cut(s)	43, 2172, 2173, 2436, 2545, 2546, 2615, 2679
PspPPI	RGGWCCY	5 cut(s)	41, 1876, 2171, 2279, 2544
PstI	CTGCAG	1 cut(s)	952
PstNI	CAGNNNCTG	2 cut(s)	1922, 2734
PsuI	RGATCY	4 cut(s)	1223, 1918, 2360, 2677
RseI	CAYNNNNRTG	2 cut(s)	1244, 2822
SacI	GAGCTC	1 cut(s)	1890
SalI	GTCGAC	1 cut(s)	364
SaqAI	TTAA	7 cut(s)	324, 570, 804, 858, 993, 2310, 2562
SatI	GCNGC	6 cut(s)	123, 437, 876, 1167, 1281, 2133
SchI	GAGTC	6 cut(s)	62, 341, 472, 1316, 1826, 2234
ScrFI	CCNGG	8 cut(s)	62, 191, 645, 1587, 2541, 2542, 2673, 2735
SduI	GDGCHC	4 cut(s)	432, 1830, 1890, 2272
SexAI	ACCWGGT	1 cut(s)	189
SfcI	CTRYAG	4 cut(s)	225, 948, 1569, 2664
Sfr274I	CTCGAG	1 cut(s)	2271
SinI	GGWCC	9 cut(s)	41, 58, 1313, 1876, 2139, 2156, 2171, 2279, 2544
SlaI	CTCGAG	1 cut(s)	2271
SmaI	CCCGGG	1 cut(s)	2542
SmiMI	CAYNNNNRTG	2 cut(s)	1244, 2822
SmlI	CTYRAG	4 cut(s)	596, 2271, 2608, 2622
SmoI	CTYRAG	4 cut(s)	596, 2271, 2608, 2622
Sse9I	AATT	9 cut(s)	607, 655, 759, 806, 1175, 2001, 2095, 2744, 2801
SseBI	AGGCCT	1 cut(s)	1189
SsiI	CCGC	4 cut(s)	122, 230, 311, 1019
SspI	AATATT	1 cut(s)	322
SspMI	CTAG	2 cut(s)	2079, 2109
SstI	GAGCTC	1 cut(s)	1890
StuI	AGGCCT	1 cut(s)	1189
StyD4I	CCNGG	8 cut(s)	60, 189, 643, 1585, 2539, 2540, 2671, 2733
StyI	CCWWGG	1 cut(s)	815
TaiI	ACGT	3 cut(s)	359, 968, 2392
TaqII	GACCGA	1 cut(s)	2144
TasI	AATT	9 cut(s)	607, 655, 759, 806, 1175, 2001, 2095, 2744, 2801
TatI	WGTACW	3 cut(s)	592, 898, 1854
TauI	GCSGC	1 cut(s)	125
TfiI	GAWTC	5 cut(s)	1450, 1862, 1952, 2585, 2725
Tru1I	TTAA	7 cut(s)	324, 570, 804, 858, 993, 2310, 2562
Tru9I	TTAA	7 cut(s)	324, 570, 804, 858, 993, 2310, 2562
TscAI	CASTG	8 cut(s)	535, 1246, 1447, 1707, 1713, 1747, 2267, 2497
TseFI	GTSAC	2 cut(s)	509, 1150
TseI	GCWGC	5 cut(s)	436, 875, 1166, 1280, 2132
Tsp45I	GTSAC	2 cut(s)	509, 1150
TspGWI	ACGGA	2 cut(s)	1670, 2618
TspMI	CCCGGG	1 cut(s)	2540
TspRI	CASTG	8 cut(s)	535, 1246, 1447, 1707, 1713, 1747, 2267, 2497
VpaK11BI	GGWCC	9 cut(s)	41, 58, 1313, 1876, 2139, 2156, 2171, 2279, 2544
VspI	ATTAAT	1 cut(s)	858
XapI	RAATTY	2 cut(s)	2744, 2801
XceI	RCATGY	1 cut(s)	2519
XhoI	CTCGAG	1 cut(s)	2271
XmaI	CCCGGG	1 cut(s)	2540
XmiI	GTMKAC	4 cut(s)	365, 491, 1437, 1671
XspI	CTAG	2 cut(s)	2079, 2109
Zsp2I	ATGCAT	1 cut(s)	1426

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.