Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0002643

Physical Location & Seq

Genomic Coordinates Forward (+)

49143520 .. 49151104

Gene Structure 7585 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG350900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2745 bp

ATGGATGATAGTCTATACGACGAGTTCGGGAACTATATAGGACCCGAAATCGAGTCGGACCAGGAGAGTGATAGGGAGGAAGAAGATGAGGAGCTTCCGGATAGGTTAGAAGATAGAGGTGCGGCATCCGACGGTGAGGATGCTCAGAATGGGTGGATGACTGCCTCTAATGATGTAGATATGGACAACCAGGTTGTTCTTGCTGAGGATAAGAAGTATTATCCTACAGCGGAGGAGGTGTACGGCGAAGATGTTGAGACCTTGGTCATGGATGAAGATGCACAGCCCCTTGAACAGCCGATTATCAAGCCGGTTCGGAATATTAAGTTTGAGGTTGGTGTCAAGGACTCCTCGACGTATGTGTCCACGCAGTTTCTTGTTGGTCTTATGTCGAACCCTGCTCTGGTTCGGAATGTGGCGCTGGTGGGGCACTTGCAGCATGGGAAGACGGTGTTTATGGATATGTTGGTGGAACAGACTCATCATATGTCCACTTTTGATGCAAATAGTGATAAGCACATGAGGTACACTGATACGAGGATTGATGAACAAGAGAGGAGGATATCTATTAAGGCTGTTCCCATGTCCCTTGTACTTGAGGATAGCAATTCAAAGTCGTACCTGTGCAATGTCATGGATACTCCTGGTCATGTCAATTTCTCTGATGAAATGACTGCTTCACTTAGGCTCGCTGATGGTGCTGTCCTCATTGTTGATGCTGCAGAAGGGATGATGGTAAATACTGAGAGGGCTATACGCCATGCAATCCAAGAGCGCCTACCAATTGTGGTTGTAATCAACAAGGTTGACAGATTGATAACAGAACTTAAATTGCCCCCAAGGGATGCTTACTTCAAGCTTAGGCATACTCTGGAAGTGATTAATAACCACATAACGGCTGCCTCTTCAACTGCTGGTCATGTACAAGTTATTGATCCTGTTGCTGGAAACGTATGCTTTGCAAGCGCTACTGCAGGATGGTCGTTCACGTTGCAATCATTTGCGAAGCTATATGTTAAGCTTCATGGGATCGCTTTTGACGCGGATCAGTTTGCTTCTCGTCTATGGGGAGATATGTACTATCATCCTGAGGACAGGGCTTTCAGGAGAAAACCCCCTGCAAGTGGAGGAGAAAGGTCGTTTGTCCAGTTTGTGCTTGAACCCCTGTATAAAATTTATAGCCAGGTGATTGGAGAACATAAAAAGAGTGTTGAGGCAACTCTTGCTGAACTTGGTGTCACCCTTAGCAATGCAGCTTACAAATTGAATGTTAGGCCTCTACTGAGGTTGGCTTGTAGCTCGGTTTTTGGATCTGCCTCGGGCTTCACTGATATGCTTGTGCAACATATTCCTTCTCCTAAAGATGCTGCAACAAGGAAAGTCGACCACACATATACTGGACCGAAAGACTCTTTGATTTACAAAGCCATGAAAACTTGTGATCCCAATGGCCCATTGATGGTAAATGTTACCAAGCTGTATCCCAAGTCCGATTGTAGCGTGTTCGATGCATTTGGTAGGGTATACAGTGGTAAGATTCAGACAGGACAGTCAGTACGAGTATTGGGAGAAGGGTATTCACCTGACGATGAGGAGGACATGACAGTCAAAGAAGTAACGAAGCTATGGCTTTACCAAGCGCGAGATAGGATACCTATAGCTGAAGCTCCTCCTGGTACTTGGGTTCTCATCGAAGGTGTGGATGCTTCCATAATGAAAACTGCCACTCTGTGTAATGAGAGTTATGGCGAGGATGTATACATATTCCGTCCTCTTCAGTTCAATACACTGCCAGTGGTGAAAACGGCAACTGAGCCTCTAAATCCCAGTGAGTTGCCAAAAATGGTGGAGGGTCTTAGAAAGATCAGCAAGAGTTATCCTCTTGCCATCACCAAAGTTGAGGAGTCTGGGGAGCACACAATCTTAGGTACAGGAGAACTGTACTTGGATTCCATCATGAAGGACCTCAGAGAGCTCTACTCCGAAGTAGAAGTCAAGGTTGCAGATCCTGTTGTTTCATTCTGCGAAACAGTTGTGGAATCTTCATCAATGAAGTGTTTTGCTGAAACACCAAACAAGAAGAATAAAATTACTATGATTGCAGAGCCATTAGAGAGAGGACTTGCGGAGGACATTGAGAAAGGTGTTGTAAGCATTGATTGGTCTAGGAGAGATATAGGTAATTTCTTCCAGACTAGGTATGAGTGGGATGTTCTTGCTGCACGGTCCATATGGGCATTCGGTCCTGATAAGCAGGGACCCAACATACTACTTGATGACACACTTTCAAGCGAAGTTGACAAGAGCTTGCTGAATGCTGTGAAAGACTCCATTGTGCAAGGGTTTCAGTGGGGTGCTCGAGAAGGACCTCTGTGTGATGAACCCATCAGAAATGTTAAGTTCAAGATAGTTGATGCAAGGATTGCAACTGAGCCGTTGCATCGTGGATCTGGTCAAATCATTCCCACATCTCGACGTGTGGCCTATTCATCTTTCCTAATGGCAACCCCACGACTCATGGAGCCAGTATATTATGTGGAGATACAAACGCCAATCGACTGTATATCCGCAATCTACACTGTTTTATCTCGCAGGCGTGGACATGTTACAGCCGATGTTCCTCAACCCGGGACCCCAGCTTACATTGTTAAGGCATTTTTACCCGTCATAGATGTGGTGACCAGAGAGGGGGGTCCCACAGCGCCGCGACCCCGGGCCAATGAGAAATCGACATGTCGCGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005730 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0007275 GO:0008150 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0010154 GO:0015030 GO:0016020 GO:0016604 GO:0016607 GO:0022414 GO:0030054 GO:0030312 GO:0030532 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043900 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045694 GO:0046540 GO:0048316 GO:0048367 GO:0048437 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0070013 GO:0071007 GO:0071013 GO:0071944 GO:0090567 GO:0097525 GO:0097526 GO:0099402 GO:0120114 GO:1902494 GO:1990904 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

EFG_C EFG_IV EFTUD2 GTP_EFTU GTP_EFTU_D2

Protein Analysis

914

Amino Acids

101.87

Weight (kDa)

5.07

Isoelectric Point (pI)

37.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EFTUD2	PF16004	3 - 116	9.8e-37	116 kDa U5 small nuclear ribonucleoprotein component N-terminus
GTP_EFTU	PF00009	136 - 330	5.9e-42	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	504 - 578	2.5e-10	Elongation factor Tu domain 2
EFG_IV	PF03764	724 - 835	8.7e-19	Elongation factor G, domain IV
EFG_C	PF00679	838 - 893	1.4e-08	Elongation factor G C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002643)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06220 AT1G06220 AT1G06220 AT5G25230 AT5G25230
fragaria_vesca	FvH4_2g24581 FvH4_7g03790 FvH4_7g03790 FvH4_7g03790
malus_domestica	MD02G1013600.v1.1 MD15G1160400.v1.1
prunus_persica	Prupe.3G036300_v2.0.a1
pyrus_communis	pycom02g01260 pycom15g14420
rosa_chinensis	RchiOBHm_Chr1g0327101 RchiOBHm_Chr1g0327121 RchiOBHm_Chr2g0127751
rosa_laevigata	RLG00000012029 RLG00000012030 RLG00000030043
rosa_multiflora	Rmu_sc0000795.1_g000050 Rmu_sc0004240.1_g000007
rosa_roxburghii	Rroxscaffold_4G00323330 Rroxscaffold_6G00399840 Rroxscaffold_6G00400180
rosa_rugosa	Rorug01G0063500
rosa_samantha	Rh1AG080100 Rh1AG080300 Rh1AG080700 Rh1AG080900 Rh1BG064100 Rh1CG078700 Rh1CG078800 Rh1DG085100 Rh1DG085200 Rh2AG323400 Rh2BG332600 Rh2DG350900
rosa_wichuraiana	Rw1G006400 Rw2G026250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	361, 1549, 1604
AccI	GTMKAC	3 cut(s)	1383, 1524, 1758
AccII	CGCG	4 cut(s)	1043, 1642, 2707, 2739
AccIII	TCCGGA	1 cut(s)	97
AciI	CCGC	6 cut(s)	122, 230, 1043, 2126, 2568, 2705
AclWI	GGATC	7 cut(s)	929, 1037, 1053, 1318, 1436, 2000, 2455
AcsI	RAATTY	1 cut(s)	1173
AcuI	CTGAAG	2 cut(s)	1683, 1760
AdeI	CACNNNGTG	1 cut(s)	1731
AfeI	AGCGCT	1 cut(s)	967
AfiI	CCNNNNNNNGG	7 cut(s)	403, 895, 944, 1124, 1459, 2627, 2688
AflIII	ACRYGT	3 cut(s)	2476, 2602, 2732
AgsI	TTSAA	9 cut(s)	293, 612, 856, 909, 1160, 1267, 1783, 2289, 2404
AjiI	CACGTC	1 cut(s)	2477
AjnI	CCWGG	5 cut(s)	60, 189, 643, 1182, 1672
Alw21I	GWGCWC	3 cut(s)	1917, 1977, 2359
Alw26I	GTCTC	1 cut(s)	251
AlwI	GGATC	7 cut(s)	929, 1037, 1053, 1318, 1436, 2000, 2455
AlwNI	CAGNNNCTG	1 cut(s)	2009
Ama87I	CYCGRG	4 cut(s)	1318, 2358, 2627, 2712
Aor13HI	TCCGGA	1 cut(s)	97
Aor51HI	AGCGCT	1 cut(s)	967
AoxI	GGCC	4 cut(s)	1274, 1450, 2481, 2715
ApeKI	GCWGC	6 cut(s)	436, 719, 899, 1253, 1367, 2219
ApoI	RAATTY	1 cut(s)	1173
ArsI	GACNNNNNNTTYG	2 cut(s)	1977, 2009
AseI	ATTAAT	1 cut(s)	882
AspLEI	GCGC	6 cut(s)	421, 777, 968, 1642, 2704, 2741
AsuC2I	CCSGG	4 cut(s)	2628, 2629, 2713, 2714
AsuHPI	GGTGA	7 cut(s)	146, 1198, 1231, 1572, 1810, 1882, 2689
AvaI	CYCGRG	4 cut(s)	1318, 2358, 2627, 2712
AxyI	CCTNAGG	1 cut(s)	1089
BaeGI	GKGCMC	1 cut(s)	432
BaeI	ACNNNNGTAYC	2 cut(s)	1668, 1701
BanII	GRGCYC	1 cut(s)	1977
BbsI	GAAGAC	1 cut(s)	452
Bbv12I	GWGCWC	3 cut(s)	1917, 1977, 2359
BbvCI	CCTCAGC	1 cut(s)	204
BbvI	GCAGC	6 cut(s)	448, 706, 886, 1265, 1354, 2206
BccI	CCATC	7 cut(s)	689, 727, 972, 1453, 1895, 1961, 2393
BceAI	ACGGC	4 cut(s)	259, 912, 1821, 2419
BcgI	CGANNNNNNTGC	2 cut(s)	963, 997
BciT130I	CCWGG	5 cut(s)	62, 191, 645, 1184, 1674
BciVI	GTATCC	3 cut(s)	631, 1491, 1644
BcnI	CCSGG	4 cut(s)	2628, 2629, 2713, 2714
BcoDI	GTCTC	1 cut(s)	251
BfaI	CTAG	2 cut(s)	2166, 2196
BfmI	CTRYAG	4 cut(s)	225, 720, 972, 1656
BfoI	RGCGCY	4 cut(s)	422, 778, 969, 2705
BfuI	GTATCC	3 cut(s)	631, 1491, 1644
BisI	GCNGC	8 cut(s)	123, 437, 720, 900, 1254, 1368, 2220, 2705
BlsI	GCNGC	8 cut(s)	124, 438, 721, 901, 1255, 1369, 2221, 2706
Bme1390I	CCNGG	9 cut(s)	62, 191, 645, 1184, 1674, 2628, 2629, 2713, 2714
BmeT110I	CYCGRG	4 cut(s)	1318, 2358, 2627, 2712
BmgBI	CACGTC	1 cut(s)	2477
BmiI	GGNNCC	8 cut(s)	43, 2259, 2260, 2523, 2632, 2633, 2694, 2695
BmrFI	CCNGG	9 cut(s)	62, 191, 645, 1184, 1674, 2628, 2629, 2713, 2714
BmrI	ACTGGG	1 cut(s)	1821
BmuI	ACTGGG	1 cut(s)	1821
BoxI	GACNNNNGTC	1 cut(s)	263
BpiI	GAAGAC	1 cut(s)	452
BplI	GAGNNNNNCTC	4 cut(s)	1864, 1896, 1964, 1996
Bpu10I	CCTNAGC	3 cut(s)	204, 860, 1244
BpuEI	CTTGAG	1 cut(s)	617
BpuMI	CCSGG	4 cut(s)	2628, 2629, 2713, 2714
BsaI	GGTCTC	1 cut(s)	251
BsaJI	CCNNGG	6 cut(s)	261, 839, 1317, 2627, 2711, 2712
BsaWI	WCCGGW	1 cut(s)	97
BsaXI	ACNNNNNCTCC	2 cut(s)	224, 254
Bsc4I	CCNNNNNNNGG	7 cut(s)	403, 895, 944, 1124, 1459, 2627, 2688
Bse118I	RCCGGY	1 cut(s)	310
Bse1I	ACTGG	5 cut(s)	1147, 1402, 1793, 1827, 2525
Bse21I	CCTNAGG	1 cut(s)	1089
Bse3DI	GCAATG	2 cut(s)	634, 1255
BseAI	TCCGGA	1 cut(s)	97
BseBI	CCWGG	5 cut(s)	62, 191, 645, 1184, 1674
BseDI	CCNNGG	6 cut(s)	261, 839, 1317, 2627, 2711, 2712
BseLI	CCNNNNNNNGG	7 cut(s)	403, 895, 944, 1124, 1459, 2627, 2688
BseMI	GCAATG	2 cut(s)	634, 1255
BseMII	CTCAG	8 cut(s)	158, 195, 735, 1080, 1274, 1803, 1981, 2421
BseNI	ACTGG	5 cut(s)	1147, 1402, 1793, 1827, 2525
BseRI	GAGGAG	8 cut(s)	104, 248, 340, 571, 1143, 1607, 1659, 1916
BseSI	GKGCMC	1 cut(s)	432
BseXI	GCAGC	6 cut(s)	448, 706, 886, 1265, 1354, 2206
BseYI	CCCAGC	1 cut(s)	2635
BsgI	GTGCAG	1 cut(s)	2205
Bsh1236I	CGCG	4 cut(s)	1043, 1642, 2707, 2739
BshFI	GGCC	4 cut(s)	1276, 1452, 2483, 2717
BsiHKAI	GWGCWC	3 cut(s)	1917, 1977, 2359
BsiHKCI	CYCGRG	4 cut(s)	1318, 2358, 2627, 2712
BsiSI	CCGG	4 cut(s)	98, 311, 2628, 2713
BslFI	GGGAC	4 cut(s)	571, 2271, 2644, 2679
BslI	CCNNNNNNNGG	7 cut(s)	403, 895, 944, 1124, 1459, 2627, 2688
BsmAI	GTCTC	1 cut(s)	251
BsmFI	GGGAC	4 cut(s)	571, 2271, 2644, 2679
BsmI	GAATGC	2 cut(s)	2237, 2320
BsnI	GGCC	4 cut(s)	1276, 1452, 2483, 2717
Bso31I	GGTCTC	1 cut(s)	251
BsoBI	CYCGRG	4 cut(s)	1318, 2358, 2627, 2712
Bsp1286I	GDGCHC	4 cut(s)	432, 1917, 1977, 2359
Bsp13I	TCCGGA	1 cut(s)	97
Bsp1407I	TGTACA	1 cut(s)	922
Bsp143I	GATC	8 cut(s)	934, 1029, 1045, 1310, 1441, 1863, 2005, 2447
BspACI	CCGC	6 cut(s)	122, 230, 1043, 2126, 2568, 2705
BspANI	GGCC	4 cut(s)	1276, 1452, 2483, 2717
BspCNI	CTCAG	8 cut(s)	157, 196, 736, 1081, 1275, 1804, 1980, 2422
BspEI	TCCGGA	1 cut(s)	97
BspFNI	CGCG	4 cut(s)	1043, 1642, 2707, 2739
BspHI	TCATGA	1 cut(s)	1956
BspLI	GGNNCC	8 cut(s)	43, 2259, 2260, 2523, 2632, 2633, 2694, 2695
BspMAI	CTGCAG	2 cut(s)	724, 976
BspPI	GGATC	7 cut(s)	929, 1037, 1053, 1318, 1436, 2000, 2455
BspTNI	GGTCTC	1 cut(s)	251
BsrDI	GCAATG	2 cut(s)	634, 1255
BsrFI	RCCGGY	1 cut(s)	310
BsrGI	TGTACA	1 cut(s)	922
BsrI	ACTGG	5 cut(s)	1147, 1402, 1793, 1827, 2525
BssAI	RCCGGY	1 cut(s)	310
BssECI	CCNNGG	6 cut(s)	261, 839, 1317, 2627, 2711, 2712
BssMI	GATC	8 cut(s)	934, 1029, 1045, 1310, 1441, 1863, 2005, 2447
BssNAI	GTATAC	2 cut(s)	1525, 1759
BssT1I	CCWWGG	2 cut(s)	261, 839
Bst1107I	GTATAC	2 cut(s)	1525, 1759
Bst2UI	CCWGG	5 cut(s)	62, 191, 645, 1184, 1674
Bst6I	CTCTTC	2 cut(s)	910, 1779
BstAPI	GCANNNNNTGC	2 cut(s)	1223, 2423
BstAUI	TGTACA	1 cut(s)	922
BstC8I	GCNNGC	4 cut(s)	690, 964, 2309, 2594
BstEII	GGTNACC	1 cut(s)	2677
BstFNI	CGCG	4 cut(s)	1043, 1642, 2707, 2739
BstH2I	RGCGCY	4 cut(s)	422, 778, 969, 2705
BstHHI	GCGC	6 cut(s)	421, 777, 968, 1642, 2704, 2741
BstKTI	GATC	8 cut(s)	937, 1032, 1048, 1313, 1444, 1866, 2008, 2450
BstMAI	GTCTC	1 cut(s)	251
BstMBI	GATC	8 cut(s)	934, 1029, 1045, 1310, 1441, 1863, 2005, 2447
BstMWI	GCNNNNNNNGC	7 cut(s)	427, 436, 698, 963, 1040, 1223, 2423
BstNI	CCWGG	5 cut(s)	62, 191, 645, 1184, 1674
BstNSI	RCATGY	2 cut(s)	2606, 2736
BstPAI	GACNNNNGTC	1 cut(s)	263
BstPI	GGTNACC	1 cut(s)	2677
BstSCI	CCNGG	9 cut(s)	60, 189, 643, 1182, 1672, 2626, 2627, 2711, 2712
BstSFI	CTRYAG	4 cut(s)	225, 720, 972, 1656
BstSLI	GKGCMC	1 cut(s)	432
BstUI	CGCG	4 cut(s)	1043, 1642, 2707, 2739
BstV1I	GCAGC	6 cut(s)	448, 706, 886, 1265, 1354, 2206
BstV2I	GAAGAC	1 cut(s)	452
BstX2I	RGATCY	3 cut(s)	1310, 2005, 2447
BstXI	CCANNNNNNTGG	1 cut(s)	1190
BstYI	RGATCY	3 cut(s)	1310, 2005, 2447
BstZ17I	GTATAC	2 cut(s)	1525, 1759
Bsu36I	CCTNAGG	1 cut(s)	1089
BsuI	GTATCC	3 cut(s)	631, 1491, 1644
BsuRI	GGCC	4 cut(s)	1276, 1452, 2483, 2717
BtrI	CACGTC	1 cut(s)	2477
BtsI	GCAGTG	1 cut(s)	1787
BtsIMutI	CAGTG	8 cut(s)	528, 1326, 1534, 1787, 1800, 1834, 2354, 2577
Cac8I	GCNNGC	4 cut(s)	690, 964, 2309, 2594
CaiI	CAGNNNCTG	1 cut(s)	2009
CciI	TCATGA	1 cut(s)	1956
CfoI	GCGC	6 cut(s)	421, 777, 968, 1642, 2704, 2741
Cfr10I	RCCGGY	1 cut(s)	310
Cfr9I	CCCGGG	2 cut(s)	2627, 2712
CseI	GACGC	1 cut(s)	1049
CsiI	ACCWGGT	1 cut(s)	189
CspCI	CAANNNNNGTGG	2 cut(s)	1827, 1862
DpnI	GATC	8 cut(s)	936, 1031, 1047, 1312, 1443, 1865, 2007, 2449
DpnII	GATC	8 cut(s)	934, 1029, 1045, 1310, 1441, 1863, 2005, 2447
DraIII	CACNNNGTG	1 cut(s)	1731
DrdI	GACNNNNNNGTC	3 cut(s)	361, 1549, 1604
DseDI	GACNNNNNNGTC	3 cut(s)	361, 1549, 1604
Eam1104I	CTCTTC	2 cut(s)	910, 1779
EarI	CTCTTC	2 cut(s)	910, 1779
Ecl136II	GAGCTC	1 cut(s)	1975
Eco130I	CCWWGG	2 cut(s)	261, 839
Eco147I	AGGCCT	1 cut(s)	1276
Eco24I	GRGCYC	1 cut(s)	1977
Eco31I	GGTCTC	1 cut(s)	251
Eco32I	GATATC	1 cut(s)	564
Eco47III	AGCGCT	1 cut(s)	967
Eco53kI	GAGCTC	1 cut(s)	1975
Eco57I	CTGAAG	2 cut(s)	1683, 1760
Eco81I	CCTNAGG	1 cut(s)	1089
Eco88I	CYCGRG	4 cut(s)	1318, 2358, 2627, 2712
Eco91I	GGTNACC	1 cut(s)	2677
EcoICRI	GAGCTC	1 cut(s)	1975
EcoO109I	RGGNCCY	6 cut(s)	41, 1963, 2258, 2366, 2631, 2693
EcoO65I	GGTNACC	1 cut(s)	2677
EcoRII	CCWGG	5 cut(s)	60, 189, 643, 1182, 1672
EcoRV	GATATC	1 cut(s)	564
EcoT14I	CCWWGG	2 cut(s)	261, 839
EcoT22I	ATGCAT	1 cut(s)	1513
EcoT38I	GRGCYC	1 cut(s)	1977
ErhI	CCWWGG	2 cut(s)	261, 839
FalI	AAGNNNNNCTT	2 cut(s)	832, 864
FaqI	GGGAC	4 cut(s)	571, 2271, 2644, 2679
FauNDI	CATATG	2 cut(s)	486, 2231
FblI	GTMKAC	3 cut(s)	1383, 1524, 1758
Fnu4HI	GCNGC	8 cut(s)	123, 437, 720, 900, 1254, 1368, 2220, 2705
FriOI	GRGCYC	1 cut(s)	1977
Fsp4HI	GCNGC	8 cut(s)	123, 437, 720, 900, 1254, 1368, 2220, 2705
FspBI	CTAG	2 cut(s)	2166, 2196
GlaI	GCGC	6 cut(s)	420, 776, 967, 1641, 2703, 2740
GluI	GCNGC	8 cut(s)	123, 437, 720, 900, 1254, 1368, 2220, 2705
GsaI	CCCAGC	1 cut(s)	2639
HaeII	RGCGCY	4 cut(s)	422, 778, 969, 2705
HaeIII	GGCC	4 cut(s)	1276, 1452, 2483, 2717
HapII	CCGG	4 cut(s)	98, 311, 2628, 2713
HgaI	GACGC	1 cut(s)	1049
HhaI	GCGC	6 cut(s)	421, 777, 968, 1642, 2704, 2741
Hin6I	GCGC	6 cut(s)	419, 775, 966, 1640, 2702, 2739
HinP1I	GCGC	6 cut(s)	419, 775, 966, 1640, 2702, 2739
HincII	GTYRAC	3 cut(s)	808, 1384, 2299
HindII	GTYRAC	3 cut(s)	808, 1384, 2299
HindIII	AAGCTT	2 cut(s)	857, 1019
HpaII	CCGG	4 cut(s)	98, 311, 2628, 2713
HphI	GGTGA	7 cut(s)	146, 1198, 1231, 1572, 1810, 1882, 2689
Hpy99I	CGWCG	4 cut(s)	23, 134, 358, 2478
HpyAV	CCTTC	6 cut(s)	719, 1362, 1565, 1688, 1954, 2357
HpyCH4IV	ACGT	4 cut(s)	356, 951, 989, 2476
HpyF10VI	GCNNNNNNNGC	7 cut(s)	427, 436, 698, 963, 1040, 1223, 2423
HpySE526I	ACGT	4 cut(s)	356, 951, 989, 2476
HspAI	GCGC	6 cut(s)	419, 775, 966, 1640, 2702, 2739
KflI	GGGWCCC	3 cut(s)	2258, 2631, 2693
Kpn2I	TCCGGA	1 cut(s)	97
Kzo9I	GATC	8 cut(s)	934, 1029, 1045, 1310, 1441, 1863, 2005, 2447
LmnI	GCTCC	4 cut(s)	91, 1672, 1912, 2521
Lsp1109I	GCAGC	6 cut(s)	448, 706, 886, 1265, 1354, 2206
MabI	ACCWGGT	1 cut(s)	189
MaeI	CTAG	2 cut(s)	2166, 2196
MaeII	ACGT	4 cut(s)	356, 951, 989, 2476
MaeIII	GTNAC	5 cut(s)	1237, 1468, 1615, 2605, 2677
MalI	GATC	8 cut(s)	936, 1031, 1047, 1312, 1443, 1865, 2007, 2449
MboI	GATC	8 cut(s)	934, 1029, 1045, 1310, 1441, 1863, 2005, 2447
MfeI	CAATTG	1 cut(s)	783
MflI	RGATCY	3 cut(s)	1310, 2005, 2447
MhlI	GDGCHC	4 cut(s)	432, 1917, 1977, 2359
MluCI	AATT	8 cut(s)	607, 655, 783, 830, 1173, 1262, 2088, 2182
MlyI	GAGTC	7 cut(s)	62, 341, 472, 1403, 1913, 2321, 2508
MmeI	TCCRAC	2 cut(s)	36, 153
Mph1103I	ATGCAT	1 cut(s)	1513
MroI	TCCGGA	1 cut(s)	97
MseI	TTAA	7 cut(s)	324, 570, 828, 882, 1017, 2397, 2649
MslI	CAYNNNNRTG	2 cut(s)	1331, 2672
MspA1I	CMGCKG	1 cut(s)	230
MspI	CCGG	4 cut(s)	98, 311, 2628, 2713
MspR9I	CCNGG	9 cut(s)	62, 191, 645, 1184, 1674, 2628, 2629, 2713, 2714
MunI	CAATTG	1 cut(s)	783
Mva1269I	GAATGC	2 cut(s)	2237, 2320
MvaI	CCWGG	5 cut(s)	62, 191, 645, 1184, 1674
MvnI	CGCG	4 cut(s)	1043, 1642, 2707, 2739
MwoI	GCNNNNNNNGC	7 cut(s)	427, 436, 698, 963, 1040, 1223, 2423
NciI	CCSGG	4 cut(s)	2628, 2629, 2713, 2714
NdeI	CATATG	2 cut(s)	486, 2231
NdeII	GATC	8 cut(s)	934, 1029, 1045, 1310, 1441, 1863, 2005, 2447
NlaIV	GGNNCC	8 cut(s)	43, 2259, 2260, 2523, 2632, 2633, 2694, 2695
NmuCI	GTSAC	2 cut(s)	1237, 2677
NsiI	ATGCAT	1 cut(s)	1513
NspI	RCATGY	2 cut(s)	2606, 2736
PaeR7I	CTCGAG	1 cut(s)	2358
PagI	TCATGA	1 cut(s)	1956
PceI	AGGCCT	1 cut(s)	1276
PciI	ACATGT	2 cut(s)	2602, 2732
PctI	GAATGC	2 cut(s)	2237, 2320
PfeI	GAWTC	3 cut(s)	1537, 1949, 2039
PkrI	GCNGC	8 cut(s)	124, 438, 721, 901, 1255, 1369, 2221, 2706
PleI	GAGTC	7 cut(s)	61, 341, 472, 1403, 1912, 2321, 2508
PpsI	GAGTC	7 cut(s)	61, 341, 472, 1403, 1912, 2321, 2508
PpuMI	RGGWCCY	6 cut(s)	41, 1963, 2258, 2366, 2631, 2693
PscI	ACATGT	2 cut(s)	2602, 2732
PshAI	GACNNNNGTC	1 cut(s)	263
PshBI	ATTAAT	1 cut(s)	882
Psp124BI	GAGCTC	1 cut(s)	1977
Psp5II	RGGWCCY	6 cut(s)	41, 1963, 2258, 2366, 2631, 2693
Psp6I	CCWGG	5 cut(s)	60, 189, 643, 1182, 1672
PspEI	GGTNACC	1 cut(s)	2677
PspFI	CCCAGC	1 cut(s)	2635
PspGI	CCWGG	5 cut(s)	60, 189, 643, 1182, 1672
PspN4I	GGNNCC	8 cut(s)	43, 2259, 2260, 2523, 2632, 2633, 2694, 2695
PspPPI	RGGWCCY	6 cut(s)	41, 1963, 2258, 2366, 2631, 2693
PstI	CTGCAG	2 cut(s)	724, 976
PstNI	CAGNNNCTG	1 cut(s)	2009
PsuI	RGATCY	3 cut(s)	1310, 2005, 2447
RseI	CAYNNNNRTG	2 cut(s)	1331, 2672
SacI	GAGCTC	1 cut(s)	1977
SalI	GTCGAC	1 cut(s)	1382
SaqAI	TTAA	7 cut(s)	324, 570, 828, 882, 1017, 2397, 2649
SatI	GCNGC	8 cut(s)	123, 437, 720, 900, 1254, 1368, 2220, 2705
Sau3AI	GATC	8 cut(s)	934, 1029, 1045, 1310, 1441, 1863, 2005, 2447
SchI	GAGTC	7 cut(s)	62, 341, 472, 1403, 1913, 2321, 2508
ScrFI	CCNGG	9 cut(s)	62, 191, 645, 1184, 1674, 2628, 2629, 2713, 2714
SduI	GDGCHC	4 cut(s)	432, 1917, 1977, 2359
SexAI	ACCWGGT	1 cut(s)	189
SfcI	CTRYAG	4 cut(s)	225, 720, 972, 1656
Sfr274I	CTCGAG	1 cut(s)	2358
SlaI	CTCGAG	1 cut(s)	2358
SmaI	CCCGGG	2 cut(s)	2629, 2714
SmiMI	CAYNNNNRTG	2 cut(s)	1331, 2672
SmlI	CTYRAG	2 cut(s)	596, 2358
SmoI	CTYRAG	2 cut(s)	596, 2358
Sse9I	AATT	8 cut(s)	607, 655, 783, 830, 1173, 1262, 2088, 2182
SseBI	AGGCCT	1 cut(s)	1276
SsiI	CCGC	6 cut(s)	122, 230, 1043, 2126, 2568, 2705
SspI	AATATT	1 cut(s)	322
SspMI	CTAG	2 cut(s)	2166, 2196
SstI	GAGCTC	1 cut(s)	1977
StuI	AGGCCT	1 cut(s)	1276
StyD4I	CCNGG	9 cut(s)	60, 189, 643, 1182, 1672, 2626, 2627, 2711, 2712
StyI	CCWWGG	2 cut(s)	261, 839
TaiI	ACGT	4 cut(s)	359, 954, 992, 2479
TaqII	GACCGA	2 cut(s)	1417, 2231
TasI	AATT	8 cut(s)	607, 655, 783, 830, 1173, 1262, 2088, 2182
TatI	WGTACW	4 cut(s)	592, 922, 1077, 1941
TauI	GCSGC	2 cut(s)	125, 2707
TfiI	GAWTC	3 cut(s)	1537, 1949, 2039
Tru1I	TTAA	7 cut(s)	324, 570, 828, 882, 1017, 2397, 2649
Tru9I	TTAA	7 cut(s)	324, 570, 828, 882, 1017, 2397, 2649
TscAI	CASTG	8 cut(s)	535, 1333, 1534, 1794, 1800, 1834, 2354, 2584
TseFI	GTSAC	2 cut(s)	1237, 2677
TseI	GCWGC	6 cut(s)	436, 719, 899, 1253, 1367, 2219
Tsp45I	GTSAC	2 cut(s)	1237, 2677
TspGWI	ACGGA	1 cut(s)	1757
TspMI	CCCGGG	2 cut(s)	2627, 2712
TspRI	CASTG	8 cut(s)	535, 1333, 1534, 1794, 1800, 1834, 2354, 2584
VspI	ATTAAT	1 cut(s)	882
XapI	RAATTY	1 cut(s)	1173
XceI	RCATGY	2 cut(s)	2606, 2736
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1394
XhoI	CTCGAG	1 cut(s)	2358
XmaI	CCCGGG	2 cut(s)	2627, 2712
XmiI	GTMKAC	3 cut(s)	1383, 1524, 1758
XspI	CTAG	2 cut(s)	2166, 2196
Zsp2I	ATGCAT	1 cut(s)	1513

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.