Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002643

Physical Location & Seq

Genomic Coordinates Forward (+)

861308 .. 866359

Gene Structure 5052 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1013600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2967 bp

ATGGATGACAGTCTGTACGATGAGTTTGGGAATTACATAGGCCCGGAAATCGAGTCTGATCAAGAGAGTGATAGAGAGGACGAGGATGAGGAGCTGCCCGATAGGCAAGAGGATAGGGATGCATCAGATGGCGATGATGCAGCTGCTGGTCCCAATGGGTGGCTGACTGCGTCCAATGATGTCGATATGGATAACCAGGTTGTCCTTGCTGAGGATAAAAAGTATTATCCAACCGCGGAAGAGGTCTATGGTGAAGATGTTGAGACATTGGTTATGGATGAAGATGCACAGCCTCTGGAACAACCCATTATTAAGCCTGTAAGGAATGTCAAGTTTGAGGTTGGTGTCAAGGATTCTTCCACCTACGTGTCGAACCAGTTCCTTTTGGGACTCATGTCGAACCCCTCTCTGGTTAGGAATGTAGCACTTGTGGGGCACTTGCAGCACGGGAAGACAGTGTTCATGGATATGTTGGTGGAGCAGACTCACCATATGTCTACTTTTGATTCAAATAGCGATAAGCACATGAGGTACACGGATACAAGGATTGATGAGCAAGAGAGGAGGATATCCATTAAGACAGTTCCTATGTCCCTTGTACTCGAGGATAGCAAGTGTAAATCGTATTTGTGCAACATCATGGATACGCCTGGTCATGTCAATTTCTCTGATGAAATGACTGCAGCCCTTAGGCTTGCCGATGGTGCTGTCTTGATTGTGGACGCTGCAGAAGGAATGATGGTAAATACCGAGAGGGCTATACGCCATGCAATCCAAGAGCGGCTACCAATTGTGGTTGTAATCAACAAGGTTGACAGATTGATAACAGAACTCAAATTGCCCCCAAGAGATGCTTACTTCAAGATAAGGCATACTTTGGACGTCATTAATACCCACATAACTGCGGCCTCTTCAACTGCTGGCAATGTACAAATTATTGACCCTGTTGCAGGAAATGTTTGCTTTGCAAGTGCTACCGCAGGATGGTCATTCACGTTGCAATCGTTTGCAAAGCTATATCTTAAACTCCATGGGGCTGATAACACGGACGTAGATAAGTTTGCCTCTCGTCTCTGGGGAGATATGTACTATCATCCAGATGACAGGAGTTTTAGGAGAAAACCCCCTGCCAGTGGAGGCGAAAGGTCATTTGTCCAGTTTGTGCTCGAACCCCTGTATAAAATTTATAGCCAGGTGATTGGGGAACATAAAAAGAGTGTGGAGGCAACTCTTGCTGAATTTGGTGTCACCCTCAGCAATGCAGCTTACAAGTTGAATGTTAGACCTCTGCTGAGGTTGGCTTGTAGCTCAGTTTTCGGATATGCCTCAGGCTTTACTGATATGCTGGTTCAACATATTCCTTCTCCTAAAGGTGCCGCAATGAGGAAAGTAGACCATATATATACTGGACCAAAAGACTCTTTGATTTACAAGGCCATGAAGGATTGTGATCCTGATGGTCCTTTGATGGTCAATGTGACAAAATTGTATCCCAAGTCCGACTGTAGTGTGTTTGATGCATTTGGTAGGGTTTATAGTGGCAAGATTCAGACAGGGCAGACTGTGCGGGTATTGGGAGAAGGCTATTCACCAGAGGATGAGGAGGACATGACAGTCAAAGAGGTGACTAAGTTATGGCTTTATCAAGCTCGAGATAGGATACCAATAGCCGAGGCTCCTCCTGGTACTTGGGTTCTTATTGAAGGTGTGGATGCTTCTATAATGAAAACTGCCACCATCTGTAATGAATATTACGACGAGGATGTATACATCTTCCGTCCTCTTCAGTTCAATACTCTGCCAGTTGTGAAAACAGCAACTGAGCCACTAAATCCAAGTGAGCTGCCAAAGATGGTGGAGGGACTAAGAAAGATAAGCAAGAGTTATCCTCTTGCAATCACTAAAGTTGAGGAGTCTGGGGAGCACACAATATTAGGTACAGGAGAACTCTACTTGGATTCAATAATGAAGGATCTTAGAGAGTTATATTCAGAAGTAGAAGTCAAGGTTGCGGATCCTGTTGTTTCATTTTGTGAAACAGTGGTGGAGTCTTCGTCAATGAAATGTTTTGCTGAAACACCCAATAAGAAGAATAAAATAACTATGATTGCGGAGCCATTGGAGAGAGGACTTGCTGAGGACATTGAGAATGGTGTTGTAAGCATTGATTGGTCTCGGAAGGATATAGGCAATTTTTTCCAGACTAGATATGAGTGGGATGTTCTTGCTGCACGTTCCATATGGGCATTTGGCCCTGATAAGCAGGGACCTAACATTCTATTAGATGACACTCTTTCCACTGAAGTCGACAAAAGCTTGCTGAATGCTGTGAAGGATTCAATTGTTCAAGGGTTTCAATGGGGTGCTCGAGAAGGACCCCTTTGTGATGAACCAATCAGAAATGTCAAGTTCAAAATTGTGGATGCAAGGATTGCCCAAGAGCCATTGCATCGTGGATCTGGCCAGATCATTCCCACATCTCGACGTGTGGCCTATTCATCTTTCCTGATGGCAACACCGCGGCTTATGGAGCCAGTATATTATGTGGAGATACAAACACCCATCGACTGTATTTCTGCAATCTACACTGTTCTATCTCGCAGGCGTGGACATGTTACTGCTGATGTTCCTCAACCTGGGACCCCTGCTTATATCGTCAAGGCGTTCTTACCAGTGATCGAATCATTTGGCTTTGAAACTGATTTGAGGTACCATACCCAAGGACAGGCTTTCTGCCTCTCAGTGTTTGATCACTGGGCTATAGTCCCTGGGGATCCACTTGATAAGAGCATCGTTCTGCGACCCCTTGAACCTGCCCCAATTCAGCATCTGGCCCGTGAGTTTATGGTCAAGACAAGACGTAGAAAGGGAATGAGCGAGGATGTGAGCATCAACAAATTCTTCGACGAGGCTATGATGGTGGAGCTGGCTCAGCAGGCAGCTGATCTTCATCTGCAAATGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005730 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0007275 GO:0008150 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0010154 GO:0015030 GO:0016020 GO:0016604 GO:0016607 GO:0022414 GO:0030054 GO:0030312 GO:0030532 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043900 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045694 GO:0046540 GO:0048316 GO:0048367 GO:0048437 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0070013 GO:0071007 GO:0071013 GO:0071944 GO:0090567 GO:0097525 GO:0097526 GO:0099402 GO:0120114 GO:1902494 GO:1990904 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

EFG_C EFG_IV EFTUD2 GTP_EFTU GTP_EFTU_D2

Protein Analysis

989

Amino Acids

110.82

Weight (kDa)

4.97

Isoelectric Point (pI)

38.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EFTUD2	PF16004	3 - 118	1.1e-36	116 kDa U5 small nuclear ribonucleoprotein component N-terminus
GTP_EFTU	PF00009	138 - 332	1.4e-41	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	507 - 581	5.8e-11	Elongation factor Tu domain 2
EFG_IV	PF03764	724 - 838	2.6e-19	Elongation factor G, domain IV
EFG_C	PF00679	841 - 929	3.7e-22	Elongation factor G C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002643)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06220 AT1G06220 AT1G06220 AT5G25230 AT5G25230
fragaria_vesca	FvH4_2g24581 FvH4_7g03790 FvH4_7g03790 FvH4_7g03790
malus_domestica	MD02G1013600.v1.1 MD15G1160400.v1.1
prunus_persica	Prupe.3G036300_v2.0.a1
pyrus_communis	pycom02g01260 pycom15g14420
rosa_chinensis	RchiOBHm_Chr1g0327101 RchiOBHm_Chr1g0327121 RchiOBHm_Chr2g0127751
rosa_laevigata	RLG00000012029 RLG00000012030 RLG00000030043
rosa_multiflora	Rmu_sc0000795.1_g000050 Rmu_sc0004240.1_g000007
rosa_roxburghii	Rroxscaffold_4G00323330 Rroxscaffold_6G00399840 Rroxscaffold_6G00400180
rosa_rugosa	Rorug01G0063500
rosa_samantha	Rh1AG080100 Rh1AG080300 Rh1AG080700 Rh1AG080900 Rh1BG064100 Rh1CG078700 Rh1CG078800 Rh1DG085100 Rh1DG085200 Rh2AG323400 Rh2BG332600 Rh2DG350900
rosa_wichuraiana	Rw1G006400 Rw2G026250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1613
AatII	GACGTC	1 cut(s)	885
Acc36I	ACCTGC	1 cut(s)	2821
Acc65I	GGTACC	1 cut(s)	2709
AccB1I	GGYRCC	2 cut(s)	1373, 2709
AccB7I	CCANNNNNTGG	1 cut(s)	159
AccBSI	CCGCTC	1 cut(s)	781
AccI	GTMKAC	4 cut(s)	497, 1392, 1767, 2307
AccII	CGCG	2 cut(s)	236, 2521
AclWI	GGATC	7 cut(s)	1445, 1980, 2009, 2022, 2464, 2768, 2781
AcoI	YGGCCR	1 cut(s)	2461
AcsI	RAATTY	3 cut(s)	1182, 1238, 2897
AcuI	CTGAAG	2 cut(s)	1769, 2322
AcyI	GRCGYC	1 cut(s)	882
AfaI	GTAC	8 cut(s)	17, 533, 600, 930, 1088, 1687, 1939, 2711
AfiI	CCNNNNNNNGG	9 cut(s)	159, 211, 409, 950, 984, 1133, 1383, 2636, 2725
AflIII	ACRYGT	3 cut(s)	366, 2485, 2611
AjiI	CACGTC	1 cut(s)	2486
AjnI	CCWGG	6 cut(s)	195, 649, 1191, 1681, 2635, 2767
AleI	CACNNNNGTG	1 cut(s)	365
Alw21I	GWGCWC	3 cut(s)	1167, 1926, 2368
Alw26I	GTCTC	3 cut(s)	257, 1076, 2178
AlwI	GGATC	7 cut(s)	1445, 1980, 2009, 2022, 2464, 2768, 2781
AlwNI	CAGNNNCTG	4 cut(s)	146, 295, 1820, 2830
Ama87I	CYCGRG	3 cut(s)	602, 1650, 2367
AoxI	GGCC	7 cut(s)	40, 906, 1434, 2251, 2461, 2490, 2832
ApoI	RAATTY	3 cut(s)	1182, 1238, 2897
AseI	ATTAAT	1 cut(s)	888
Asp700I	GAANNNNTTC	1 cut(s)	377
Asp718I	GGTACC	1 cut(s)	2709
AspS9I	GGNCC	9 cut(s)	41, 149, 1409, 1460, 2252, 2267, 2375, 2640, 2833
AsuC2I	CCSGG	1 cut(s)	44
AsuHPI	GGTGA	6 cut(s)	263, 479, 1207, 1240, 1581, 1636
AvaI	CYCGRG	3 cut(s)	602, 1650, 2367
AvaII	GGWCC	6 cut(s)	149, 1409, 1460, 2267, 2375, 2640
AxyI	CCTNAGG	2 cut(s)	689, 1327
BaeGI	GKGCMC	1 cut(s)	438
BaeI	ACNNNNGTAYC	2 cut(s)	1677, 1710
BalI	TGGCCA	1 cut(s)	2463
BamHI	GGATCC	2 cut(s)	2014, 2773
BanI	GGYRCC	2 cut(s)	1373, 2709
BbsI	GAAGAC	2 cut(s)	458, 2043
Bbv12I	GWGCWC	3 cut(s)	1167, 1926, 2368
BbvCI	CCTCAGC	4 cut(s)	210, 1253, 1292, 2136
BciT130I	CCWGG	6 cut(s)	197, 651, 1193, 1683, 2637, 2769
BciVI	GTATCC	4 cut(s)	532, 637, 1500, 1653
BclI	TGATCA	2 cut(s)	58, 2749
BcnI	CCSGG	1 cut(s)	44
BcoDI	GTCTC	3 cut(s)	257, 1076, 2178
BfaI	CTAG	1 cut(s)	2205
BfmI	CTRYAG	4 cut(s)	681, 726, 1504, 2760
BfuAI	ACCTGC	1 cut(s)	2821
BfuI	GTATCC	4 cut(s)	532, 637, 1500, 1653
BglI	GCCNNNNNGGC	1 cut(s)	103
BlpI	GCTNAGC	1 cut(s)	2931
Bme1390I	CCNGG	7 cut(s)	44, 197, 651, 1193, 1683, 2637, 2769
Bme18I	GGWCC	6 cut(s)	149, 1409, 1460, 2267, 2375, 2640
BmeT110I	CYCGRG	3 cut(s)	602, 1650, 2367
BmgBI	CACGTC	1 cut(s)	2486
BmgT120I	GGNCC	9 cut(s)	41, 149, 1409, 1460, 2252, 2267, 2375, 2640, 2833
BmrFI	CCNGG	7 cut(s)	44, 197, 651, 1193, 1683, 2637, 2769
BmrI	ACTGGG	1 cut(s)	2764
BmuI	ACTGGG	1 cut(s)	2764
BoxI	GACNNNNGTC	1 cut(s)	394
BpiI	GAAGAC	2 cut(s)	458, 2043
BplI	GAGNNNNNCTC	2 cut(s)	1873, 1905
Bpu10I	CCTNAGC	4 cut(s)	210, 1253, 1292, 2136
Bpu1102I	GCTNAGC	1 cut(s)	2931
BpuMI	CCSGG	1 cut(s)	44
BsaAI	YACGTR	1 cut(s)	367
BsaBI	GATNNNNATC	2 cut(s)	1449, 2949
BsaHI	GRCGYC	1 cut(s)	882
BsaI	GGTCTC	1 cut(s)	2178
BsaJI	CCNNGG	8 cut(s)	234, 1030, 1671, 2519, 2636, 2719, 2767, 2768
BsaXI	ACNNNNNCTCC	2 cut(s)	2039, 2069
Bsc4I	CCNNNNNNNGG	9 cut(s)	159, 211, 409, 950, 984, 1133, 1383, 2636, 2725
Bse1I	ACTGG	8 cut(s)	376, 1131, 1156, 1411, 1802, 2534, 2672, 2759
Bse21I	CCTNAGG	2 cut(s)	689, 1327
Bse3DI	GCAATG	4 cut(s)	931, 1264, 1386, 2444
Bse8I	GATNNNNATC	2 cut(s)	1449, 2949
BseBI	CCWGG	6 cut(s)	197, 651, 1193, 1683, 2637, 2769
BseDI	CCNNGG	8 cut(s)	234, 1030, 1671, 2519, 2636, 2719, 2767, 2768
BseJI	GATNNNNATC	2 cut(s)	1449, 2949
BseLI	CCNNNNNNNGG	9 cut(s)	159, 211, 409, 950, 984, 1133, 1383, 2636, 2725
BseMI	GCAATG	4 cut(s)	931, 1264, 1386, 2444
BseMII	CTCAG	9 cut(s)	201, 1267, 1283, 1323, 1341, 1812, 2127, 2754, 2945
BseNI	ACTGG	8 cut(s)	376, 1131, 1156, 1411, 1802, 2534, 2672, 2759
BseRI	GAGGAG	5 cut(s)	104, 577, 1616, 1668, 1925
BseSI	GKGCMC	1 cut(s)	438
BsgI	GTGCAG	1 cut(s)	2214
Bsh1236I	CGCG	2 cut(s)	236, 2521
BshFI	GGCC	7 cut(s)	42, 908, 1436, 2253, 2463, 2492, 2834
BshNI	GGYRCC	2 cut(s)	1373, 2709
BsiHKAI	GWGCWC	3 cut(s)	1167, 1926, 2368
BsiHKCI	CYCGRG	3 cut(s)	602, 1650, 2367
BsiSI	CCGG	1 cut(s)	44
BslFI	GGGAC	7 cut(s)	135, 402, 577, 1875, 2280, 2653, 2750
BslI	CCNNNNNNNGG	9 cut(s)	159, 211, 409, 950, 984, 1133, 1383, 2636, 2725
BsmAI	GTCTC	3 cut(s)	257, 1076, 2178
BsmBI	CGTCTC	1 cut(s)	1076
BsmFI	GGGAC	7 cut(s)	135, 402, 577, 1875, 2280, 2653, 2750
BsmI	GAATGC	1 cut(s)	2329
BsnI	GGCC	7 cut(s)	42, 908, 1436, 2253, 2463, 2492, 2834
Bso31I	GGTCTC	1 cut(s)	2178
BsoBI	CYCGRG	3 cut(s)	602, 1650, 2367
Bsp1286I	GDGCHC	4 cut(s)	438, 1167, 1926, 2368
Bsp1407I	TGTACA	1 cut(s)	928
Bsp1720I	GCTNAGC	1 cut(s)	2931
Bsp19I	CCATGG	1 cut(s)	1030
BspANI	GGCC	7 cut(s)	42, 908, 1436, 2253, 2463, 2492, 2834
BspCNI	CTCAG	9 cut(s)	202, 1266, 1284, 1322, 1340, 1813, 2128, 2753, 2944
BspFNI	CGCG	2 cut(s)	236, 2521
BspMAI	CTGCAG	2 cut(s)	685, 730
BspMI	ACCTGC	1 cut(s)	2821
BspPI	GGATC	7 cut(s)	1445, 1980, 2009, 2022, 2464, 2768, 2781
BspT107I	GGYRCC	2 cut(s)	1373, 2709
BspTNI	GGTCTC	1 cut(s)	2178
BsrBI	CCGCTC	1 cut(s)	781
BsrDI	GCAATG	4 cut(s)	931, 1264, 1386, 2444
BsrGI	TGTACA	1 cut(s)	928
BsrI	ACTGG	8 cut(s)	376, 1131, 1156, 1411, 1802, 2534, 2672, 2759
BssECI	CCNNGG	8 cut(s)	234, 1030, 1671, 2519, 2636, 2719, 2767, 2768
BssNAI	GTATAC	1 cut(s)	1768
BssNI	GRCGYC	1 cut(s)	882
BssT1I	CCWWGG	2 cut(s)	1030, 2719
Bst1107I	GTATAC	1 cut(s)	1768
Bst2UI	CCWGG	6 cut(s)	197, 651, 1193, 1683, 2637, 2769
Bst4CI	ACNGT	9 cut(s)	11, 457, 583, 1505, 1564, 1615, 2041, 2570, 2590
Bst6I	CTCTTC	3 cut(s)	234, 916, 1788
BstACI	GRCGYC	1 cut(s)	882
BstAPI	GCANNNNNTGC	3 cut(s)	1232, 1564, 2432
BstAUI	TGTACA	1 cut(s)	928
BstBAI	YACGTR	1 cut(s)	367
BstC8I	GCNNGC	6 cut(s)	696, 922, 2318, 2603, 2928, 2937
BstDSI	CCRYGG	3 cut(s)	234, 1030, 2519
BstENI	CCTNNNNNAGG	2 cut(s)	209, 948
BstFNI	CGCG	2 cut(s)	236, 2521
BstMAI	GTCTC	3 cut(s)	257, 1076, 2178
BstMWI	GCNNNNNNNGC	9 cut(s)	103, 442, 704, 1232, 1564, 2432, 2530, 2932, 2936
BstNI	CCWGG	6 cut(s)	197, 651, 1193, 1683, 2637, 2769
BstNSI	RCATGY	1 cut(s)	2615
BstPAI	GACNNNNGTC	1 cut(s)	394
BstSCI	CCNGG	7 cut(s)	42, 195, 649, 1191, 1681, 2635, 2767
BstSFI	CTRYAG	4 cut(s)	681, 726, 1504, 2760
BstSLI	GKGCMC	1 cut(s)	438
BstUI	CGCG	2 cut(s)	236, 2521
BstV2I	GAAGAC	2 cut(s)	458, 2043
BstX2I	RGATCY	4 cut(s)	1972, 2014, 2456, 2773
BstXI	CCANNNNNNTGG	1 cut(s)	1199
BstYI	RGATCY	4 cut(s)	1972, 2014, 2456, 2773
BstZ17I	GTATAC	1 cut(s)	1768
Bsu36I	CCTNAGG	2 cut(s)	689, 1327
BsuI	GTATCC	4 cut(s)	532, 637, 1500, 1653
BsuRI	GGCC	7 cut(s)	42, 908, 1436, 2253, 2463, 2492, 2834
BtgI	CCRYGG	3 cut(s)	234, 1030, 2519
BtgZI	GCGATG	1 cut(s)	147
BtrI	CACGTC	1 cut(s)	2486
BtsIMutI	CAGTG	8 cut(s)	462, 1138, 2046, 2298, 2586, 2679, 2748, 2752
BveI	ACCTGC	1 cut(s)	2821
Cac8I	GCNNGC	6 cut(s)	696, 922, 2318, 2603, 2928, 2937
CaiI	CAGNNNCTG	4 cut(s)	146, 295, 1820, 2830
Cfr13I	GGNCC	9 cut(s)	41, 149, 1409, 1460, 2252, 2267, 2375, 2640, 2833
Cfr42I	CCGCGG	2 cut(s)	237, 2522
CseI	GACGC	2 cut(s)	159, 731
CsiI	ACCWGGT	1 cut(s)	195
Csp6I	GTAC	8 cut(s)	16, 532, 599, 929, 1087, 1686, 1938, 2710
CspCI	CAANNNNNGTGG	2 cut(s)	1836, 1871
CviQI	GTAC	8 cut(s)	16, 532, 599, 929, 1087, 1686, 1938, 2710
DrdI	GACNNNNNNGTC	1 cut(s)	1613
DseDI	GACNNNNNNGTC	1 cut(s)	1613
EaeI	YGGCCR	1 cut(s)	2461
Eam1104I	CTCTTC	3 cut(s)	234, 916, 1788
EarI	CTCTTC	3 cut(s)	234, 916, 1788
Eco130I	CCWWGG	2 cut(s)	1030, 2719
Eco31I	GGTCTC	1 cut(s)	2178
Eco32I	GATATC	1 cut(s)	570
Eco47I	GGWCC	6 cut(s)	149, 1409, 1460, 2267, 2375, 2640
Eco57I	CTGAAG	2 cut(s)	1769, 2322
Eco81I	CCTNAGG	2 cut(s)	689, 1327
Eco88I	CYCGRG	3 cut(s)	602, 1650, 2367
EcoNI	CCTNNNNNAGG	2 cut(s)	209, 948
EcoO109I	RGGNCCY	3 cut(s)	2267, 2375, 2640
EcoRII	CCWGG	6 cut(s)	195, 649, 1191, 1681, 2635, 2767
EcoRV	GATATC	1 cut(s)	570
EcoT14I	CCWWGG	2 cut(s)	1030, 2719
EcoT22I	ATGCAT	2 cut(s)	124, 1522
ErhI	CCWWGG	2 cut(s)	1030, 2719
Esp3I	CGTCTC	1 cut(s)	1076
FalI	AAGNNNNNCTT	8 cut(s)	838, 870, 859, 891, 2364, 2396, 2651, 2683
FaqI	GGGAC	7 cut(s)	135, 402, 577, 1875, 2280, 2653, 2750
FauI	CCCGC	1 cut(s)	1560
FauNDI	CATATG	2 cut(s)	492, 2240
FbaI	TGATCA	2 cut(s)	58, 2749
FblI	GTMKAC	4 cut(s)	497, 1392, 1767, 2307
FspBI	CTAG	1 cut(s)	2205
HaeIII	GGCC	7 cut(s)	42, 908, 1436, 2253, 2463, 2492, 2834
HapII	CCGG	1 cut(s)	44
HgaI	GACGC	2 cut(s)	159, 731
Hin1I	GRCGYC	1 cut(s)	882
HincII	GTYRAC	2 cut(s)	814, 2308
HindII	GTYRAC	2 cut(s)	814, 2308
HindIII	AAGCTT	1 cut(s)	2314
HpaII	CCGG	1 cut(s)	44
HphI	GGTGA	6 cut(s)	263, 479, 1207, 1240, 1581, 1636
Hpy166II	GTNNAC	8 cut(s)	498, 534, 721, 814, 1393, 1768, 2308, 2609
Hpy188I	TCNGA	9 cut(s)	58, 127, 670, 1319, 1501, 1551, 1993, 2178, 2399
Hpy8I	GTNNAC	8 cut(s)	498, 534, 721, 814, 1393, 1768, 2308, 2609
Hpy99I	CGWCG	3 cut(s)	1760, 2487, 2909
HpyAV	CCTTC	9 cut(s)	725, 1371, 1435, 1574, 1697, 1963, 2173, 2326, 2366
HpyCH4III	ACNGT	9 cut(s)	11, 457, 583, 1505, 1564, 1615, 2041, 2570, 2590
HpyCH4IV	ACGT	7 cut(s)	366, 882, 995, 1050, 2233, 2485, 2860
HpyF10VI	GCNNNNNNNGC	9 cut(s)	103, 442, 704, 1232, 1564, 2432, 2530, 2932, 2936
HpySE526I	ACGT	7 cut(s)	366, 882, 995, 1050, 2233, 2485, 2860
Hsp92I	GRCGYC	1 cut(s)	882
KflI	GGGWCCC	1 cut(s)	2640
KpnI	GGTACC	1 cut(s)	2713
Ksp22I	TGATCA	2 cut(s)	58, 2749
KspI	CCGCGG	2 cut(s)	237, 2522
LmnI	GCTCC	7 cut(s)	91, 478, 1681, 1921, 2113, 2530, 2923
MabI	ACCWGGT	1 cut(s)	195
MaeI	CTAG	1 cut(s)	2205
MaeII	ACGT	7 cut(s)	366, 882, 995, 1050, 2233, 2485, 2860
MaeIII	GTNAC	4 cut(s)	1246, 1477, 1624, 2614
MbiI	CCGCTC	1 cut(s)	781
MfeI	CAATTG	2 cut(s)	789, 2340
MflI	RGATCY	4 cut(s)	1972, 2014, 2456, 2773
MhlI	GDGCHC	4 cut(s)	438, 1167, 1926, 2368
MlsI	TGGCCA	1 cut(s)	2463
MluNI	TGGCCA	1 cut(s)	2463
MlyI	GAGTC	6 cut(s)	62, 384, 478, 1412, 1922, 2057
MmeI	TCCRAC	2 cut(s)	254, 1524
Mox20I	TGGCCA	1 cut(s)	2463
Mph1103I	ATGCAT	2 cut(s)	124, 1522
MroXI	GAANNNNTTC	1 cut(s)	377
MscI	TGGCCA	1 cut(s)	2463
MseI	TTAA	4 cut(s)	312, 576, 888, 1023
MslI	CAYNNNNRTG	3 cut(s)	365, 467, 1098
Msp20I	TGGCCA	1 cut(s)	2463
MspA1I	CMGCKG	4 cut(s)	143, 236, 2521, 2942
MspI	CCGG	1 cut(s)	44
MspR9I	CCNGG	7 cut(s)	44, 197, 651, 1193, 1683, 2637, 2769
MunI	CAATTG	2 cut(s)	789, 2340
Mva1269I	GAATGC	1 cut(s)	2329
MvaI	CCWGG	6 cut(s)	197, 651, 1193, 1683, 2637, 2769
MvnI	CGCG	2 cut(s)	236, 2521
MwoI	GCNNNNNNNGC	9 cut(s)	103, 442, 704, 1232, 1564, 2432, 2530, 2932, 2936
NciI	CCSGG	1 cut(s)	44
NcoI	CCATGG	1 cut(s)	1030
NdeI	CATATG	2 cut(s)	492, 2240
NmeAIII	GCCGAG	1 cut(s)	1696
NmuCI	GTSAC	3 cut(s)	1246, 1477, 1624
NsiI	ATGCAT	2 cut(s)	124, 1522
NspI	RCATGY	1 cut(s)	2615
OliI	CACNNNNGTG	1 cut(s)	365
PaeR7I	CTCGAG	3 cut(s)	602, 1650, 2367
PasI	CCCWGGG	1 cut(s)	2768
PciI	ACATGT	1 cut(s)	2611
PcsI	WCGNNNNNNNCGW	1 cut(s)	1001
PctI	GAATGC	1 cut(s)	2329
PdmI	GAANNNNTTC	1 cut(s)	377
PfeI	GAWTC	6 cut(s)	353, 506, 1546, 1958, 2336, 2681
PflFI	GACNNNGTC	1 cut(s)	169
PflMI	CCANNNNNTGG	1 cut(s)	159
PleI	GAGTC	6 cut(s)	61, 384, 478, 1412, 1921, 2056
PpsI	GAGTC	6 cut(s)	61, 384, 478, 1412, 1921, 2056
Ppu21I	YACGTR	1 cut(s)	367
PpuMI	RGGWCCY	3 cut(s)	2267, 2375, 2640
PscI	ACATGT	1 cut(s)	2611
PshAI	GACNNNNGTC	1 cut(s)	394
PshBI	ATTAAT	1 cut(s)	888
Psp5II	RGGWCCY	3 cut(s)	2267, 2375, 2640
Psp6I	CCWGG	6 cut(s)	195, 649, 1191, 1681, 2635, 2767
PspGI	CCWGG	6 cut(s)	195, 649, 1191, 1681, 2635, 2767
PspPI	GGNCC	9 cut(s)	41, 149, 1409, 1460, 2252, 2267, 2375, 2640, 2833
PspPPI	RGGWCCY	3 cut(s)	2267, 2375, 2640
PspXI	VCTCGAGB	1 cut(s)	602
PstI	CTGCAG	2 cut(s)	685, 730
PstNI	CAGNNNCTG	4 cut(s)	146, 295, 1820, 2830
PsuI	RGATCY	4 cut(s)	1972, 2014, 2456, 2773
PsyI	GACNNNGTC	1 cut(s)	169
PvuII	CAGCTG	2 cut(s)	143, 2942
RsaI	GTAC	8 cut(s)	17, 533, 600, 930, 1088, 1687, 1939, 2711
RsaNI	GTAC	8 cut(s)	16, 532, 599, 929, 1087, 1686, 1938, 2710
RseI	CAYNNNNRTG	3 cut(s)	365, 467, 1098
SacII	CCGCGG	2 cut(s)	237, 2522
SalI	GTCGAC	1 cut(s)	2306
SaqAI	TTAA	4 cut(s)	312, 576, 888, 1023
Sau96I	GGNCC	9 cut(s)	41, 149, 1409, 1460, 2252, 2267, 2375, 2640, 2833
SchI	GAGTC	6 cut(s)	62, 384, 478, 1412, 1922, 2057
ScrFI	CCNGG	7 cut(s)	44, 197, 651, 1193, 1683, 2637, 2769
SduI	GDGCHC	4 cut(s)	438, 1167, 1926, 2368
SexAI	ACCWGGT	1 cut(s)	195
SfcI	CTRYAG	4 cut(s)	681, 726, 1504, 2760
Sfr274I	CTCGAG	3 cut(s)	602, 1650, 2367
Sfr303I	CCGCGG	2 cut(s)	237, 2522
SgrBI	CCGCGG	2 cut(s)	237, 2522
SinI	GGWCC	6 cut(s)	149, 1409, 1460, 2267, 2375, 2640
SlaI	CTCGAG	3 cut(s)	602, 1650, 2367
SmiMI	CAYNNNNRTG	3 cut(s)	365, 467, 1098
SmlI	CTYRAG	3 cut(s)	602, 1650, 2367
SmoI	CTYRAG	3 cut(s)	602, 1650, 2367
SspI	AATATT	2 cut(s)	1751, 1932
SspMI	CTAG	1 cut(s)	2205
StyD4I	CCNGG	7 cut(s)	42, 195, 649, 1191, 1681, 2635, 2767
StyI	CCWWGG	2 cut(s)	1030, 2719
TaaI	ACNGT	9 cut(s)	11, 457, 583, 1505, 1564, 1615, 2041, 2570, 2590
TaiI	ACGT	7 cut(s)	369, 885, 998, 1053, 2236, 2488, 2863
TatI	WGTACW	3 cut(s)	598, 928, 1086
TauI	GCSGC	4 cut(s)	784, 908, 1379, 2524
TfiI	GAWTC	6 cut(s)	353, 506, 1546, 1958, 2336, 2681
Tru1I	TTAA	4 cut(s)	312, 576, 888, 1023
Tru9I	TTAA	4 cut(s)	312, 576, 888, 1023
TscAI	CASTG	8 cut(s)	462, 1138, 2046, 2305, 2593, 2679, 2748, 2759
TseFI	GTSAC	3 cut(s)	1246, 1477, 1624
Tsp45I	GTSAC	3 cut(s)	1246, 1477, 1624
TspGWI	ACGGA	3 cut(s)	551, 1061, 1766
TspRI	CASTG	8 cut(s)	462, 1138, 2046, 2305, 2593, 2679, 2748, 2759
Tth111I	GACNNNGTC	1 cut(s)	169
Van91I	CCANNNNNTGG	1 cut(s)	159
VpaK11BI	GGWCC	6 cut(s)	149, 1409, 1460, 2267, 2375, 2640
VspI	ATTAAT	1 cut(s)	888
XagI	CCTNNNNNAGG	2 cut(s)	209, 948
XapI	RAATTY	3 cut(s)	1182, 1238, 2897
XceI	RCATGY	1 cut(s)	2615
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1403
XhoI	CTCGAG	3 cut(s)	602, 1650, 2367
XmiI	GTMKAC	4 cut(s)	497, 1392, 1767, 2307
XmnI	GAANNNNTTC	1 cut(s)	377
XspI	CTAG	1 cut(s)	2205
ZraI	GACGTC	1 cut(s)	883
Zsp2I	ATGCAT	2 cut(s)	124, 1522

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.