Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002643

Physical Location & Seq

Genomic Coordinates Forward (+)

8739545 .. 8743880

Gene Structure 4336 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G25230.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2922 bp

ATGGATGGTAGCTTGTATGGTGAGTGTGGTAACTATATTGGACCTGAGATTGAATCTGACAGAGATAGTGATGATAGTGTAGAAGATGAAGATCTCCAAGAGCCTGGGGGTTCTAATGGCTGGATTACCACTATCAATGAGAACCAAAATATTGTTCTTCCTGAGGATAAGAAGTACTACCCTATTGCTAAGGAGGTTTATGGTGAGGATGTTGAGACCTTGGTTATGGATGAAGATGAGCAGTCTCTTGAGCAACCCATTATCAAACCCGTTAGAGATATTAGATTTGAGGTGGGGGTCATCAAGGATCAAACAACAACGTATGTGTCAACACTGTTTCTTATCGGCCTCATGTCTAATCCTGCGCTTGTGAGGAATGTTGCTCTTGTGGGGCATCTCCAGCACGGCAAAACTGTCTTCATGGATATGTTGGTAGAGCAGACGCATCGTATGTCTACTTTTAATGCTGAAAACGACAAGCATATGAGATATACGGACACAAGAGTCGATGAGCAGGAGAGAAATATATCTATCAAGGCGGTTCCAATGTCTCTTGTCCTTGAGGACAGTAGATCCAAATCATACCTGTGCAATATCATGGATACACCTGGAAATGTCAATTTCTCTGACGAAATGACTGCTTCTTTAAGACTTGCTGATGGTGCTGTTTTCATTGTTGATGCTGCTCAAGGAGTCATGGTAAACACAGAGAGAGCTATACGGCATGCAATCCAGGATCATCTCCCTATTGTTGTTGTGATCAATAAGGTTGACAGGCTCATAACTGAGCTCAAACTACCTCCAAGGGATGCTTACTACAAGCTGAGGTATACAATTGAAGTCATTAATAACCATATATCTGCTGCTTCAACAAATGCTGCAGACTTACCTCTTATAGACCCTGCAGCTGGAAATGTATGTTTCGCCAGTGGTACAGCCGGATGGTCCTTCACATTACAATCTTTTGCTAGAATGTATGCCAAGCTTCATGGTGTGGCCATGGACGTGGATAAGTTTGCATCTAGACTTTGGGGAGATGTATATTATCACCCTGATACTAGGGTATTCAATACAAGTCCTCCAGTAGGTGGTGGAGAAAGAGCATTTGTTCAGTTTATTCTGGAGCCTCTATACAAGATATACAGCCAAGTGATAGGTGAACATAAGAAGAGTGTAGAGACCACCCTTGCAGAATTAGGAGTGACTCTGAGTAATAGTGCATATAAGCTGAACGTCAGACCTTTACTTAGGTTAGCCTGTAGTTCAGTGTTTGGTTCGGCATCAGGGTTCACTGATATGTTGGTAAAGCACATTCCTTCACCCAGAGAGGCTGCAGCTAGGAAAGTGGACCATTCATACACTGGAACCAAAGATTCTCCCATTTATGAATCAATGGTAGAATGTGATCCATCTGGACCTCTCATGGTTAATGTGACAAAGTTGTACCCCAAATCAGATACTAGTGTTTTTGATGTGTTTGGAAGAGTTTATAGTGGTAGGCTTCAAACAGGGCAAAGCGTACGTGTACTAGGAGAAGGGTATTCACCTGAGGATGAGGAGGACATGACTATAAAAGAAGTCACGAAGCTATGGATATATCAAGCTAGGTATAGAATACCAGTAAGCAGTGCCCCTCCCGGTTCTTGGGTTTTAATTGAAGGTGTCGATGCATCCATCATGAAAACTGCAACTCTGTGTAATGCAAGTTATGATGAAGATGTCTACATATTCCGGGCTCTCAAATTTAATACCCTCCCAGTAGTCAAGACTGCAACTGAGCCTTTGAATCCTAGTGAGCTGCCTAAAATGGTGGAAGGGCTTAGGAAAATTAGCAAAAGCTATCCCCTTGCGATAACCAAAGTTGAGGAGTCTGGGGAACATACTATTCTAGGCACAGGTGAGTTGTACTTGGATTCCATCATCAAGGACCTCAGGGAACTCTATTCAGAGGTCCAAGTAAAGGTTGCAGATCCTGTGGTCTCCTTCTGTGAGACAGTGGTTGAATCTTCGTCAATGAAGTGCTTTGCTGAGACACCAAACAAAAAGAACAAACTAACTATGATTGCAGAGCCATTGGATAGAGGACTTGCAGAGGATATTGAGAATGGCGTTGTAAGCATTGACTGGAACAGAGTACAACTTGGTGACTTCTTCAGGACGAAATATGACTGGGATTTACTTGCTGCACGTTCCATCTGGGCTTTTGGCCCTGACAAACAGGGGACAAACATATTATTGGATGACACCCTTCCAACTGAGGTGGACAGGAACTTGATGATGGGAGTGAAAGACTCCATTGTTCAAGGGTTCCAGTGGGGTGCTCGTGAAGGTCCCCTCTGTGATGAGCCCATCAGAAATGTAAAGTTCAAGATTGTTGATGCACGGATAGCACCTGAGCCGTTGCATAGAGGATCTGGTCAGATGATTCCAACGGCAAGACGTGTTGCATATTCAGCCTTTCTTATGGCAACTCCAAGGCTAATGGAACCTGTCTACTATGTGGAGATACAAACACCAATAGACTGTGTAACTGCCATCTACACAGTTTTGTCACGTAGACGTGGGTATGTCACATCTGATGTTCCACAACCCGGTACTCCTGCTTATATCGTGAAGGCTTTTCTGCCTGTGATAGAGTCGTTTGGGTTTGAGACAGATCTGAGGTACCACACTCAGGGCCAAGCCTTTTGCCTATCTGTGTTTGATCATTGGGCCATAGTCCCTGGAGACCCTCTAGACAAAGCCATCCAATTGCGCCCACTTGAACCCGCCCCTATTCAGCATCTAGCTCGGGAATTCATGGTCAAGACCCGCCGTAGAAAGGGAATGAGCGAAGATGTCAGCGGGAATAAATTCTTTGATGAAGCGATGATGGTAGAGCTTGCCCAGCAAACAGGCGATCTCCATCTGCAGATGATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005730 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0007275 GO:0008150 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0010154 GO:0015030 GO:0016020 GO:0016604 GO:0016607 GO:0022414 GO:0030054 GO:0030312 GO:0030532 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043900 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045694 GO:0046540 GO:0048316 GO:0048367 GO:0048437 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0070013 GO:0071007 GO:0071013 GO:0071944 GO:0090567 GO:0097525 GO:0097526 GO:0099402 GO:0120114 GO:1902494 GO:1990904 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

EFG_C EFG_IV EFTUD2 GTP_EFTU GTP_EFTU_D2

Protein Analysis

973

Amino Acids

108.8

Weight (kDa)

5.09

Isoelectric Point (pI)

41.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EFTUD2	PF16004	4 - 98	1.1e-23	116 kDa U5 small nuclear ribonucleoprotein component N-terminus
GTP_EFTU	PF00009	124 - 318	3.4e-41	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	492 - 566	6.2e-09	Elongation factor Tu domain 2
EFG_IV	PF03764	716 - 823	2.5e-18	Elongation factor G, domain IV
EFG_C	PF00679	826 - 914	1.6e-21	Elongation factor G C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002643)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06220 AT1G06220 AT1G06220 AT5G25230 AT5G25230
fragaria_vesca	FvH4_2g24581 FvH4_7g03790 FvH4_7g03790 FvH4_7g03790
malus_domestica	MD02G1013600.v1.1 MD15G1160400.v1.1
prunus_persica	Prupe.3G036300_v2.0.a1
pyrus_communis	pycom02g01260 pycom15g14420
rosa_chinensis	RchiOBHm_Chr1g0327101 RchiOBHm_Chr1g0327121 RchiOBHm_Chr2g0127751
rosa_laevigata	RLG00000012029 RLG00000012030 RLG00000030043
rosa_multiflora	Rmu_sc0000795.1_g000050 Rmu_sc0004240.1_g000007
rosa_roxburghii	Rroxscaffold_4G00323330 Rroxscaffold_6G00399840 Rroxscaffold_6G00400180
rosa_rugosa	Rorug01G0063500
rosa_samantha	Rh1AG080100 Rh1AG080300 Rh1AG080700 Rh1AG080900 Rh1BG064100 Rh1CG078700 Rh1CG078800 Rh1DG085100 Rh1DG085200 Rh2AG323400 Rh2BG332600 Rh2DG350900
rosa_wichuraiana	Rw1G006400 Rw2G026250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	503
Acc65I	GGTACC	1 cut(s)	2664
AccB1I	GGYRCC	1 cut(s)	2664
AccB7I	CCANNNNNTGG	1 cut(s)	1088
AccI	GTMKAC	5 cut(s)	455, 830, 1722, 2493, 2557
AciI	CCGC	4 cut(s)	539, 2769, 2812, 2844
AclWI	GGATC	6 cut(s)	315, 567, 744, 1400, 1964, 2419
AcoI	YGGCCR	1 cut(s)	996
AcsI	RAATTY	3 cut(s)	1742, 2795, 2852
AcuI	CTGAAG	1 cut(s)	2137
AfaI	GTAC	9 cut(s)	176, 934, 1445, 1521, 1527, 1907, 2136, 2596, 2666
AfiI	CCNNNNNNNGG	8 cut(s)	908, 1085, 1088, 1644, 1960, 2591, 2674, 2821
AflIII	ACRYGT	2 cut(s)	1522, 2440
AhlI	ACTAGT	1 cut(s)	1460
AjiI	CACGTC	3 cut(s)	1006, 2441, 2561
AjnI	CCWGG	4 cut(s)	103, 607, 732, 2722
AjuI	GAANNNNNNNTTGG	2 cut(s)	138, 170
Alw21I	GWGCWC	2 cut(s)	792, 2323
Alw26I	GTCTC	9 cut(s)	209, 249, 555, 1172, 1984, 1985, 2024, 2645, 2721
AlwI	GGATC	6 cut(s)	315, 567, 744, 1400, 1964, 2419
AlwNI	CAGNNNCTG	1 cut(s)	1973
Ama87I	CYCGRG	1 cut(s)	2790
AoxI	GGCC	5 cut(s)	346, 996, 2206, 2677, 2712
ApeKI	GCWGC	8 cut(s)	683, 863, 878, 905, 1331, 1334, 1798, 2183
ApoI	RAATTY	3 cut(s)	1742, 2795, 2852
AseI	ATTAAT	1 cut(s)	846
Asp700I	GAANNNNTTC	1 cut(s)	2852
Asp718I	GGTACC	1 cut(s)	2664
AspLEI	GCGC	2 cut(s)	367, 2757
AsuC2I	CCSGG	3 cut(s)	1638, 1733, 2592
AsuHPI	GGTGA	8 cut(s)	32, 215, 1040, 1169, 1311, 1536, 1910, 2156
AvaI	CYCGRG	1 cut(s)	2790
AvaII	GGWCC	7 cut(s)	41, 945, 1348, 1415, 1927, 1951, 2330
AxyI	CCTNAGG	3 cut(s)	162, 1548, 1931
BaeGI	GKGCMC	1 cut(s)	1633
BaeI	ACNNNNGTAYC	4 cut(s)	1047, 1080, 1449, 1482
BalI	TGGCCA	1 cut(s)	998
BanI	GGYRCC	1 cut(s)	2664
BanII	GRGCYC	3 cut(s)	792, 1738, 2349
BauI	CACGAG	1 cut(s)	2322
BbsI	GAAGAC	1 cut(s)	409
Bbv12I	GWGCWC	2 cut(s)	792, 2323
BbvCI	CCTCAGC	1 cut(s)	824
BbvI	GCAGC	8 cut(s)	670, 850, 865, 917, 1318, 1346, 1785, 2170
BceAI	ACGGC	5 cut(s)	421, 737, 2383, 2448, 2799
BcgI	CGANNNNNNTGC	6 cut(s)	428, 462, 620, 654, 2771, 2805
BciT130I	CCWGG	4 cut(s)	105, 609, 734, 2724
BciVI	GTATCC	1 cut(s)	595
BclI	TGATCA	2 cut(s)	759, 2704
BcnI	CCSGG	3 cut(s)	1638, 1733, 2592
BcoDI	GTCTC	9 cut(s)	209, 249, 555, 1172, 1984, 1985, 2024, 2645, 2721
BcuI	ACTAGT	1 cut(s)	1460
BfmI	CTRYAG	5 cut(s)	879, 903, 1258, 1332, 2909
BfuI	GTATCC	1 cut(s)	595
BglII	AGATCT	2 cut(s)	91, 2656
BisI	GCNGC	8 cut(s)	684, 864, 879, 906, 1332, 1335, 1799, 2184
BlsI	GCNGC	8 cut(s)	685, 865, 880, 907, 1333, 1336, 1800, 2185
BmcAI	AGTACT	1 cut(s)	176
Bme1390I	CCNGG	7 cut(s)	105, 609, 734, 1638, 1733, 2592, 2724
Bme18I	GGWCC	7 cut(s)	41, 945, 1348, 1415, 1927, 1951, 2330
BmeT110I	CYCGRG	1 cut(s)	2790
BmgBI	CACGTC	3 cut(s)	1006, 2441, 2561
BmiI	GGNNCC	7 cut(s)	543, 1125, 1366, 2309, 2332, 2487, 2666
BmrFI	CCNGG	7 cut(s)	105, 609, 734, 1638, 1733, 2592, 2724
BmrI	ACTGGG	2 cut(s)	1751, 2179
BmuI	ACTGGG	2 cut(s)	1751, 2179
BpiI	GAAGAC	1 cut(s)	409
BpmI	CTGGAG	4 cut(s)	383, 1065, 1142, 2745
Bpu10I	CCTNAGC	4 cut(s)	189, 824, 1820, 2394
BpuEI	CTTGAG	3 cut(s)	269, 581, 672
BpuMI	CCSGG	3 cut(s)	1638, 1733, 2592
BsaAI	YACGTR	2 cut(s)	1523, 2555
BsaBI	GATNNNNATC	3 cut(s)	90, 577, 2904
BsaI	GGTCTC	4 cut(s)	209, 1172, 1984, 2721
BsaJI	CCNNGG	6 cut(s)	104, 219, 803, 999, 2474, 2722
BsaXI	ACNNNNNCTCC	4 cut(s)	684, 714, 1063, 1093
Bsc4I	CCNNNNNNNGG	8 cut(s)	908, 1085, 1088, 1644, 1960, 2591, 2674, 2821
Bse1I	ACTGG	8 cut(s)	927, 1082, 1366, 1619, 1757, 2129, 2174, 2311
Bse21I	CCTNAGG	3 cut(s)	162, 1548, 1931
Bse8I	GATNNNNATC	3 cut(s)	90, 577, 2904
BseBI	CCWGG	4 cut(s)	105, 609, 734, 2724
BseDI	CCNNGG	6 cut(s)	104, 219, 803, 999, 2474, 2722
BseGI	GGATG	9 cut(s)	10, 214, 235, 814, 947, 1558, 1670, 2245, 2745
BseJI	GATNNNNATC	3 cut(s)	90, 577, 2904
BseLI	CCNNNNNNNGG	8 cut(s)	908, 1085, 1088, 1644, 1960, 2591, 2674, 2821
BseNI	ACTGG	8 cut(s)	927, 1082, 1366, 1619, 1757, 2129, 2174, 2311
BseRI	GAGGAG	2 cut(s)	1571, 1880
BseSI	GKGCMC	1 cut(s)	1633
BseXI	GCAGC	8 cut(s)	670, 850, 865, 917, 1318, 1346, 1785, 2170
BseYI	CCCAGC	1 cut(s)	2886
BsgI	GTGCAG	1 cut(s)	2169
BshFI	GGCC	5 cut(s)	348, 998, 2208, 2679, 2714
BshNI	GGYRCC	1 cut(s)	2664
BsiHKAI	GWGCWC	2 cut(s)	792, 2323
BsiHKCI	CYCGRG	1 cut(s)	2790
BsiSI	CCGG	4 cut(s)	939, 1638, 1732, 2592
BsiWI	CGTACG	1 cut(s)	1519
BslFI	GGGAC	3 cut(s)	2236, 2316, 2705
BslI	CCNNNNNNNGG	8 cut(s)	908, 1085, 1088, 1644, 1960, 2591, 2674, 2821
BsmAI	GTCTC	9 cut(s)	209, 249, 555, 1172, 1984, 1985, 2024, 2645, 2721
BsmFI	GGGAC	3 cut(s)	2236, 2316, 2705
BsnI	GGCC	5 cut(s)	348, 998, 2208, 2679, 2714
Bso31I	GGTCTC	4 cut(s)	209, 1172, 1984, 2721
BsoBI	CYCGRG	1 cut(s)	2790
Bsp1286I	GDGCHC	5 cut(s)	792, 1633, 1738, 2323, 2349
Bsp19I	CCATGG	1 cut(s)	999
BspACI	CCGC	4 cut(s)	539, 2769, 2812, 2844
BspANI	GGCC	5 cut(s)	348, 998, 2208, 2679, 2714
BspHI	TCATGA	1 cut(s)	1677
BspLI	GGNNCC	7 cut(s)	543, 1125, 1366, 2309, 2332, 2487, 2666
BspMAI	CTGCAG	4 cut(s)	883, 907, 1336, 2913
BspPI	GGATC	6 cut(s)	315, 567, 744, 1400, 1964, 2419
BspT107I	GGYRCC	1 cut(s)	2664
BspTNI	GGTCTC	4 cut(s)	209, 1172, 1984, 2721
BsrI	ACTGG	8 cut(s)	927, 1082, 1366, 1619, 1757, 2129, 2174, 2311
BssECI	CCNNGG	6 cut(s)	104, 219, 803, 999, 2474, 2722
BssNAI	GTATAC	1 cut(s)	831
BssSI	CACGAG	1 cut(s)	2322
BssT1I	CCWWGG	4 cut(s)	219, 803, 999, 2474
Bst1107I	GTATAC	1 cut(s)	831
Bst2BI	CACGAG	1 cut(s)	2322
Bst2UI	CCWGG	4 cut(s)	105, 609, 734, 2724
Bst4CI	ACNGT	6 cut(s)	336, 415, 569, 1996, 2525, 2545
Bst6I	CTCTTC	2 cut(s)	1163, 1477
BstBAI	YACGTR	2 cut(s)	1523, 2555
BstC8I	GCNNGC	2 cut(s)	726, 2883
BstDSI	CCRYGG	1 cut(s)	999
BstENI	CCTNNNNNAGG	1 cut(s)	1083
BstF5I	GGATG	9 cut(s)	10, 214, 235, 814, 947, 1558, 1670, 2245, 2745
BstHHI	GCGC	2 cut(s)	367, 2757
BstMAI	GTCTC	9 cut(s)	209, 249, 555, 1172, 1984, 1985, 2024, 2645, 2721
BstMWI	GCNNNNNNNGC	6 cut(s)	400, 662, 2115, 2387, 2453, 2887
BstNI	CCWGG	4 cut(s)	105, 609, 734, 2724
BstNSI	RCATGY	1 cut(s)	728
BstSCI	CCNGG	7 cut(s)	103, 607, 732, 1636, 1731, 2590, 2722
BstSFI	CTRYAG	5 cut(s)	879, 903, 1258, 1332, 2909
BstSLI	GKGCMC	1 cut(s)	1633
BstV1I	GCAGC	8 cut(s)	670, 850, 865, 917, 1318, 1346, 1785, 2170
BstV2I	GAAGAC	1 cut(s)	409
BstX2I	RGATCY	5 cut(s)	91, 572, 1969, 2411, 2656
BstXI	CCANNNNNNTGG	2 cut(s)	104, 1006
BstYI	RGATCY	5 cut(s)	91, 572, 1969, 2411, 2656
BstZ17I	GTATAC	1 cut(s)	831
Bsu36I	CCTNAGG	3 cut(s)	162, 1548, 1931
BsuI	GTATCC	1 cut(s)	595
BsuRI	GGCC	5 cut(s)	348, 998, 2208, 2679, 2714
BtgI	CCRYGG	1 cut(s)	999
BtgZI	GCGATG	1 cut(s)	2882
BtrI	CACGTC	3 cut(s)	1006, 2441, 2561
BtsCI	GGATG	9 cut(s)	10, 214, 235, 814, 947, 1558, 1670, 2245, 2745
BtsI	GCAGTG	1 cut(s)	1633
BtsIMutI	CAGTG	8 cut(s)	332, 934, 1272, 1290, 1359, 1633, 2001, 2318
Cac8I	GCNNGC	2 cut(s)	726, 2883
CaiI	CAGNNNCTG	1 cut(s)	1973
CciI	TCATGA	1 cut(s)	1677
CfoI	GCGC	2 cut(s)	367, 2757
CseI	GACGC	1 cut(s)	451
Csp6I	GTAC	9 cut(s)	175, 933, 1444, 1520, 1526, 1906, 2135, 2595, 2665
CspCI	CAANNNNNGTGG	2 cut(s)	2241, 2276
CviQI	GTAC	9 cut(s)	175, 933, 1444, 1520, 1526, 1906, 2135, 2595, 2665
DrdI	GACNNNNNNGTC	1 cut(s)	503
DseDI	GACNNNNNNGTC	1 cut(s)	503
EaeI	YGGCCR	1 cut(s)	996
Eam1104I	CTCTTC	2 cut(s)	1163, 1477
EarI	CTCTTC	2 cut(s)	1163, 1477
Ecl136II	GAGCTC	1 cut(s)	790
Eco130I	CCWWGG	4 cut(s)	219, 803, 999, 2474
Eco24I	GRGCYC	3 cut(s)	792, 1738, 2349
Eco31I	GGTCTC	4 cut(s)	209, 1172, 1984, 2721
Eco47I	GGWCC	7 cut(s)	41, 945, 1348, 1415, 1927, 1951, 2330
Eco53kI	GAGCTC	1 cut(s)	790
Eco57I	CTGAAG	1 cut(s)	2137
Eco81I	CCTNAGG	3 cut(s)	162, 1548, 1931
Eco88I	CYCGRG	1 cut(s)	2790
EcoICRI	GAGCTC	1 cut(s)	790
EcoNI	CCTNNNNNAGG	1 cut(s)	1083
EcoO109I	RGGNCCY	2 cut(s)	1927, 2330
EcoRI	GAATTC	1 cut(s)	2795
EcoRII	CCWGG	4 cut(s)	103, 607, 732, 2722
EcoT14I	CCWWGG	4 cut(s)	219, 803, 999, 2474
EcoT22I	ATGCAT	1 cut(s)	1672
EcoT38I	GRGCYC	3 cut(s)	792, 1738, 2349
ErhI	CCWWGG	4 cut(s)	219, 803, 999, 2474
FalI	AAGNNNNNCTT	2 cut(s)	796, 828
FaqI	GGGAC	3 cut(s)	2236, 2316, 2705
FauI	CCCGC	3 cut(s)	2776, 2819, 2837
FauNDI	CATATG	1 cut(s)	483
FbaI	TGATCA	2 cut(s)	759, 2704
FblI	GTMKAC	5 cut(s)	455, 830, 1722, 2493, 2557
Fnu4HI	GCNGC	8 cut(s)	684, 864, 879, 906, 1332, 1335, 1799, 2184
FokI	GGATG	9 cut(s)	17, 221, 242, 821, 954, 1565, 1657, 2252, 2732
FriOI	GRGCYC	3 cut(s)	792, 1738, 2349
Fsp4HI	GCNGC	8 cut(s)	684, 864, 879, 906, 1332, 1335, 1799, 2184
GlaI	GCGC	2 cut(s)	366, 2756
GluI	GCNGC	8 cut(s)	684, 864, 879, 906, 1332, 1335, 1799, 2184
GsaI	CCCAGC	1 cut(s)	2890
GsuI	CTGGAG	4 cut(s)	383, 1065, 1142, 2745
HaeIII	GGCC	5 cut(s)	348, 998, 2208, 2679, 2714
HapII	CCGG	4 cut(s)	939, 1638, 1732, 2592
HgaI	GACGC	1 cut(s)	451
HhaI	GCGC	2 cut(s)	367, 2757
Hin6I	GCGC	2 cut(s)	365, 2755
HinP1I	GCGC	2 cut(s)	365, 2755
HincII	GTYRAC	2 cut(s)	330, 772
HindII	GTYRAC	2 cut(s)	330, 772
HindIII	AAGCTT	1 cut(s)	983
HpaII	CCGG	4 cut(s)	939, 1638, 1732, 2592
HphI	GGTGA	8 cut(s)	32, 215, 1040, 1169, 1311, 1536, 1910, 2156
HpyAV	CCTTC	9 cut(s)	958, 1326, 1529, 1652, 1808, 1993, 2258, 2321, 2608
HpyCH4III	ACNGT	6 cut(s)	336, 415, 569, 1996, 2525, 2545
HpyCH4IV	ACGT	8 cut(s)	320, 1005, 1233, 1522, 2188, 2440, 2554, 2560
HpyF10VI	GCNNNNNNNGC	6 cut(s)	400, 662, 2115, 2387, 2453, 2887
HpySE526I	ACGT	8 cut(s)	320, 1005, 1233, 1522, 2188, 2440, 2554, 2560
HspAI	GCGC	2 cut(s)	365, 2755
KpnI	GGTACC	1 cut(s)	2668
Ksp22I	TGATCA	2 cut(s)	759, 2704
LmnI	GCTCC	1 cut(s)	1123
Lsp1109I	GCAGC	8 cut(s)	670, 850, 865, 917, 1318, 1346, 1785, 2170
MaeII	ACGT	8 cut(s)	320, 1005, 1233, 1522, 2188, 2440, 2554, 2560
MaeIII	GTNAC	8 cut(s)	29, 1201, 1432, 1579, 2144, 2527, 2550, 2569
MfeI	CAATTG	2 cut(s)	834, 2750
MflI	RGATCY	5 cut(s)	91, 572, 1969, 2411, 2656
MhlI	GDGCHC	5 cut(s)	792, 1633, 1738, 2323, 2349
MlsI	TGGCCA	1 cut(s)	998
MluCI	AATT	9 cut(s)	619, 834, 1193, 1653, 1742, 1827, 2750, 2795, 2852
MluNI	TGGCCA	1 cut(s)	998
MlyI	GAGTC	6 cut(s)	513, 702, 1198, 1877, 2285, 2645
MmeI	TCCRAC	2 cut(s)	2276, 2453
Mox20I	TGGCCA	1 cut(s)	998
Mph1103I	ATGCAT	1 cut(s)	1672
MroXI	GAANNNNTTC	1 cut(s)	2852
MscI	TGGCCA	1 cut(s)	998
MseI	TTAA	6 cut(s)	462, 647, 846, 1428, 1652, 1746
MslI	CAYNNNNRTG	4 cut(s)	425, 586, 1004, 1295
Msp20I	TGGCCA	1 cut(s)	998
MspA1I	CMGCKG	2 cut(s)	908, 2844
MspI	CCGG	4 cut(s)	939, 1638, 1732, 2592
MspR9I	CCNGG	7 cut(s)	105, 609, 734, 1638, 1733, 2592, 2724
MunI	CAATTG	2 cut(s)	834, 2750
MvaI	CCWGG	4 cut(s)	105, 609, 734, 2724
MwoI	GCNNNNNNNGC	6 cut(s)	400, 662, 2115, 2387, 2453, 2887
NciI	CCSGG	3 cut(s)	1638, 1733, 2592
NcoI	CCATGG	1 cut(s)	999
NdeI	CATATG	1 cut(s)	483
NlaIV	GGNNCC	7 cut(s)	543, 1125, 1366, 2309, 2332, 2487, 2666
NmuCI	GTSAC	6 cut(s)	1201, 1432, 1579, 2144, 2550, 2569
NsiI	ATGCAT	1 cut(s)	1672
NspI	RCATGY	1 cut(s)	728
PaeI	GCATGC	1 cut(s)	728
PagI	TCATGA	1 cut(s)	1677
PdmI	GAANNNNTTC	1 cut(s)	2852
PfeI	GAWTC	7 cut(s)	53, 1373, 1388, 1786, 1913, 2003, 2425
Pfl23II	CGTACG	1 cut(s)	1519
PflMI	CCANNNNNTGG	1 cut(s)	1088
PfoI	TCCNGGA	1 cut(s)	732
PkrI	GCNGC	8 cut(s)	685, 865, 880, 907, 1333, 1336, 1800, 2185
PleI	GAGTC	6 cut(s)	512, 701, 1198, 1876, 2285, 2644
PpsI	GAGTC	6 cut(s)	512, 701, 1198, 1876, 2285, 2644
Ppu21I	YACGTR	2 cut(s)	1523, 2555
PpuMI	RGGWCCY	2 cut(s)	1927, 2330
PshBI	ATTAAT	1 cut(s)	846
Psp124BI	GAGCTC	1 cut(s)	792
Psp5II	RGGWCCY	2 cut(s)	1927, 2330
Psp6I	CCWGG	4 cut(s)	103, 607, 732, 2722
PspFI	CCCAGC	1 cut(s)	2886
PspGI	CCWGG	4 cut(s)	103, 607, 732, 2722
PspLI	CGTACG	1 cut(s)	1519
PspN4I	GGNNCC	7 cut(s)	543, 1125, 1366, 2309, 2332, 2487, 2666
PspPPI	RGGWCCY	2 cut(s)	1927, 2330
PstI	CTGCAG	4 cut(s)	883, 907, 1336, 2913
PstNI	CAGNNNCTG	1 cut(s)	1973
PsuI	RGATCY	5 cut(s)	91, 572, 1969, 2411, 2656
PvuII	CAGCTG	1 cut(s)	908
RsaI	GTAC	9 cut(s)	176, 934, 1445, 1521, 1527, 1907, 2136, 2596, 2666
RsaNI	GTAC	9 cut(s)	175, 933, 1444, 1520, 1526, 1906, 2135, 2595, 2665
RseI	CAYNNNNRTG	4 cut(s)	425, 586, 1004, 1295
SacI	GAGCTC	1 cut(s)	792
SaqAI	TTAA	6 cut(s)	462, 647, 846, 1428, 1652, 1746
SatI	GCNGC	8 cut(s)	684, 864, 879, 906, 1332, 1335, 1799, 2184
ScaI	AGTACT	1 cut(s)	176
SchI	GAGTC	6 cut(s)	513, 702, 1198, 1877, 2285, 2645
ScrFI	CCNGG	7 cut(s)	105, 609, 734, 1638, 1733, 2592, 2724
SduI	GDGCHC	5 cut(s)	792, 1633, 1738, 2323, 2349
SfcI	CTRYAG	5 cut(s)	879, 903, 1258, 1332, 2909
SinI	GGWCC	7 cut(s)	41, 945, 1348, 1415, 1927, 1951, 2330
SmiMI	CAYNNNNRTG	4 cut(s)	425, 586, 1004, 1295
SmlI	CTYRAG	3 cut(s)	248, 560, 687
SmoI	CTYRAG	3 cut(s)	248, 560, 687
SpeI	ACTAGT	1 cut(s)	1460
SphI	GCATGC	1 cut(s)	728
Sse9I	AATT	9 cut(s)	619, 834, 1193, 1653, 1742, 1827, 2750, 2795, 2852
SsiI	CCGC	4 cut(s)	539, 2769, 2812, 2844
SspI	AATATT	1 cut(s)	151
SstI	GAGCTC	1 cut(s)	792
StyD4I	CCNGG	7 cut(s)	103, 607, 732, 1636, 1731, 2590, 2722
StyI	CCWWGG	4 cut(s)	219, 803, 999, 2474
TaaI	ACNGT	6 cut(s)	336, 415, 569, 1996, 2525, 2545
TaiI	ACGT	8 cut(s)	323, 1008, 1236, 1525, 2191, 2443, 2557, 2563
TaqI	TCGA	2 cut(s)	507, 1665
TasI	AATT	9 cut(s)	619, 834, 1193, 1653, 1742, 1827, 2750, 2795, 2852
TatI	WGTACW	4 cut(s)	174, 1525, 1905, 2134
TfiI	GAWTC	7 cut(s)	53, 1373, 1388, 1786, 1913, 2003, 2425
Tru1I	TTAA	6 cut(s)	462, 647, 846, 1428, 1652, 1746
Tru9I	TTAA	6 cut(s)	462, 647, 846, 1428, 1652, 1746
TscAI	CASTG	8 cut(s)	339, 934, 1272, 1297, 1366, 1633, 2001, 2318
TseFI	GTSAC	6 cut(s)	1201, 1432, 1579, 2144, 2550, 2569
TseI	GCWGC	8 cut(s)	683, 863, 878, 905, 1331, 1334, 1798, 2183
Tsp45I	GTSAC	6 cut(s)	1201, 1432, 1579, 2144, 2550, 2569
TspGWI	ACGGA	2 cut(s)	509, 2398
TspRI	CASTG	8 cut(s)	339, 934, 1272, 1297, 1366, 1633, 2001, 2318
Van91I	CCANNNNNTGG	1 cut(s)	1088
VpaK11BI	GGWCC	7 cut(s)	41, 945, 1348, 1415, 1927, 1951, 2330
VspI	ATTAAT	1 cut(s)	846
XagI	CCTNNNNNAGG	1 cut(s)	1083
XapI	RAATTY	3 cut(s)	1742, 2795, 2852
XbaI	TCTAGA	2 cut(s)	1022, 2734
XceI	RCATGY	1 cut(s)	728
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1358
XmiI	GTMKAC	5 cut(s)	455, 830, 1722, 2493, 2557
XmnI	GAANNNNTTC	1 cut(s)	2852
ZrmI	AGTACT	1 cut(s)	176
Zsp2I	ATGCAT	1 cut(s)	1672

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.