Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0002643

Physical Location & Seq

Genomic Coordinates Reverse (-)

4294495 .. 4300498

Gene Structure 6004 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g03790.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2958 bp

ATGGATGATAGTCTCTACGATGAGTTCGGGAACTATATAGGTCCCGAAATCGAGTCTGACCAGGAGAGTGAGAGGGAGGAAGAAGATGAGGAGCTTCCGGATAGGACGGATGATAGAGGCGCAGCATCCGACGGTGACGATGCTCCGAATGGGTGGATGACGGCTTCAAATGACGTAGACATGGACAACCAGATTGTGCTTGCTGAGGATAAGAAGTATTATCCTACTGCAGAGGAGGTTTATGGCGAAGATGTTGAGACATTGGTCATGGATGAAGATGCCATGCCGCTTGAGCAGCCGATTATCAAGCCGGTTCGGAATGTTAAGTTTGAGGTTGGTGTCAAGGACTCTTCGACTTATGTGTCCACACAGTTCCTTGTGGGGCTTATGTCGAACCCTGCGCTGGTGAGGAATGTGGCGCTTGTGGGGCACTTGCAGCATGGGAAGACGGTGTTTATGGATATGTTGGTTGAGCAGACACATCATATGTCTACTTTTGATGCGAATAGCGATAAGCACATGAGGTATACTGATACGAGGATTGACGAGCAGGAGAGGAGGATATCTATTAAGGCTGTTCCGATGTCGCTTGTGCTTGAGGATAGCAATTCAAAGTCGTACTTGTGCAATGTCATGGATACTCCTGGTCATGTCAATTTCTCTGATGAAATGACTGCTTCACTCAGGCTCGCTGATGGTGCTGTCCTCATTGTGGATGCTGCAGAAGGGATGATGGTAAATACTGAGAGGGCTATACGCCATGCAATCCAAGAGCGCCTACCAATTGTGGTTGTGATCAACAAGGTTGACAGGTTGATAACAGAACTAAAATTGCCCCCAAGGGATGCTTACTTCAAGCTTAGGCATACACTGGAAGTGATTAATAACCACATAACAGCTGCCTCTTCAACTGCTGGTCATGTACAAGTCATTGATCCTGTTGCTGGAAATGTATGCTTTGCAAGTGCTACTGCAGGATGGTCGTTCACATTGCAATCATTTGCGAAGCTCTATGTTAAGCTTCATGGGATCGCTTTTGACGCTGACCAGTTTGCTTCTCGTCTATGGGGAGATATGTATTATCATCCTGAGGACAGGTCATTCAGAAGAAAACCCCCTGTAAGTGGAGGAGAAAGGTCGTTTGTGCAGTTTGTGCTTGAACCTCTGTATAAAATTTATAGCCAGGTGATCGGAGAACATAAAAAGAGTGTTGAGGCAACTCTTGCTGAGCTTGGTATCACCCTTAGCAATGCAGCTTACAAATTGAATGTTAGGCCTCTACTGAGGTTGGCTTGTAGCTCAGTCTTTGGATCTGCCTCGGGCTTTACTGATATGCTAGTGCAACATATTCCTTCTCCTAAAGATGCTGCGACAAGGAAAGTGGACCACACATATACTGGACCAAAAGACTCTGAGATTTACAAAGCCATGAAAACATGTGATCCCAATGGCCCGTTGATGGTTAATGTTACCAAGCTGTATCCCAAGTCCGATTGTAGCGTGTTTGATGCATTTGGTAGGGTTTACTCTGGGAAGATTCAGACAGGGCAGACAGTTCGTGTACTGGGAGAAGGTTATTCACCTGATGATGAGGAGGACATGACGGTTAAAGAAGTAACAAAGCTATGGCTTTATCAAGCTCGGGATCGGATACCTATAGCTGAAGCTCTTCCTGGTACTTGGGTTCTCATCGAAGGTGTGGATGCTTCCATAATGAAGACTGCCACTCTTTGTAATGAGACTTATGATGAGGAGGCATACATATTCCGTCCACTTCAGTTCAATACACTCCCAGTGGTGAAAACGGCAACTGAGCCTCTGAATCCCAGTGAGTTGCCAAAGATGGTGGAGGGTCTTAGAAAGATCAGCAAGAGTTACCCTCTTGCCATCACCAAAGTTGAGGAGTCTGGGGAGCACACAATCTTAGGTACAGGAGAATTGTACTTGGATTCCATCATGAAGGACCTCAGAGAGCTCTACTCCGAAGTAGAAGTCAAGGTTGCAGATCCTGTTGTTTCATTCTGCGAAACAGTTGTGGAATCTTCATCAATGAAGTGTTTTGCTGAAACACCAAACAAGAAGAATAAAATAACTATGATTGCAGAACCGTTGGAGAGAGGACTTGCGGAGGACATTGAGAAAGGTGTAGTAAGCATCGATTGGTCTAGGAGAGATATAGGTAATTTCTTCCAGACTAGATATGAGTGGGATGTTCTTGCTGCAAGGTCCATATGGGCATTTGGTCCTGATAAGCAGGGACCCAACATACTACTAGATGACACACTCTCTACCGAAGTTGACAAGAGCTTACTGAATGCCGTGAAAGACTCGATTGTGCAAGGGTTTCAGTGGGGTGCTCGAGAAGGACCCCTATGTGATGAACCCATCAGAAATGTTAAGTTCAAGATAGTTGATGCAAGGATTGCACCTGAGCCTTTACATCGTGGATCTGGTCAAATCATTCCCACATCTAGACGTGTGGCCTATTCATCTTTCCTAATGGCAACCCCACGGCTCATGGAGCCGGTATATTATGTGGAGATACAAACGCCAATCGACTGTATATCTGCAATCTACACTGTTTTATCTCGCAGGCGTGGACATGTTACAGCCGATGTACCTCAACCCGGGACCCCAGCTTACATTGTTAAGGCATTTTTACCTGTCATAGAATCGTTTGGTTTTGAGACGGACTTGAGGTACCATACTCAAGGACAAGCTTTTTGCCTCTCAGTCTTTGATCATTGGGCTATAGTTCCTGGAGATCCTCTTGACAAGAGTATTGTTCTGCGACCACTTGAACCCGCACCGATTCAACACCTGGCTCGTGAATTTATGGTCAAGACAAGGCGTAGAAAGGGAATGAGTGAGGATGTGAGCATCAGCAAATTTTTCGATGAGGCCATGATGGTGGAACTGGCACAGCAGGAAGCTGATATTCACCAGCAAATGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005730 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0007275 GO:0008150 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0010154 GO:0015030 GO:0016020 GO:0016604 GO:0016607 GO:0022414 GO:0030054 GO:0030312 GO:0030532 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043900 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045694 GO:0046540 GO:0048316 GO:0048367 GO:0048437 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0070013 GO:0071007 GO:0071013 GO:0071944 GO:0090567 GO:0097525 GO:0097526 GO:0099402 GO:0120114 GO:1902494 GO:1990904 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

EFG_C EFG_IV EFTUD2 GTP_EFTU GTP_EFTU_D2

Protein Analysis

986

Amino Acids

110.41

Weight (kDa)

5.0

Isoelectric Point (pI)

39.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EFTUD2	PF16004	3 - 116	9.9e-36	116 kDa U5 small nuclear ribonucleoprotein component N-terminus
GTP_EFTU	PF00009	136 - 330	6.8e-42	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	504 - 578	2.6e-10	Elongation factor Tu domain 2
EFG_IV	PF03764	724 - 835	9.9e-19	Elongation factor G, domain IV
EFG_C	PF00679	838 - 926	3.7e-22	Elongation factor G C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002643)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06220 AT1G06220 AT1G06220 AT5G25230 AT5G25230
fragaria_vesca	FvH4_2g24581 FvH4_7g03790 FvH4_7g03790 FvH4_7g03790
malus_domestica	MD02G1013600.v1.1 MD15G1160400.v1.1
prunus_persica	Prupe.3G036300_v2.0.a1
pyrus_communis	pycom02g01260 pycom15g14420
rosa_chinensis	RchiOBHm_Chr1g0327101 RchiOBHm_Chr1g0327121 RchiOBHm_Chr2g0127751
rosa_laevigata	RLG00000012029 RLG00000012030 RLG00000030043
rosa_multiflora	Rmu_sc0000795.1_g000050 Rmu_sc0004240.1_g000007
rosa_roxburghii	Rroxscaffold_4G00323330 Rroxscaffold_6G00399840 Rroxscaffold_6G00400180
rosa_rugosa	Rorug01G0063500
rosa_samantha	Rh1AG080100 Rh1AG080300 Rh1AG080700 Rh1AG080900 Rh1BG064100 Rh1CG078700 Rh1CG078800 Rh1DG085100 Rh1DG085200 Rh2AG323400 Rh2BG332600 Rh2DG350900
rosa_wichuraiana	Rw1G006400 Rw2G026250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	361
Acc65I	GGTACC	1 cut(s)	2700
AccB1I	GGYRCC	1 cut(s)	2700
AccI	GTMKAC	3 cut(s)	177, 491, 527
AccIII	TCCGGA	1 cut(s)	97
AciI	CCGC	3 cut(s)	287, 2126, 2805
AclWI	GGATC	8 cut(s)	929, 1037, 1318, 1436, 1653, 2000, 2455, 2759
AcsI	RAATTY	3 cut(s)	1173, 2831, 2888
AcuI	CTGAAG	2 cut(s)	1683, 1760
AfaI	GTAC	8 cut(s)	620, 924, 1563, 1678, 1930, 1943, 2619, 2702
AfiI	CCNNNNNNNGG	6 cut(s)	403, 712, 944, 1124, 1459, 2627
AflIII	ACRYGT	3 cut(s)	1436, 2476, 2602
AjiI	CACGTC	1 cut(s)	2477
AjnI	CCWGG	6 cut(s)	60, 643, 1182, 1672, 2758, 2820
Alw21I	GWGCWC	3 cut(s)	1917, 1977, 2359
Alw26I	GTCTC	4 cut(s)	17, 251, 1733, 2681
AlwI	GGATC	8 cut(s)	929, 1037, 1318, 1436, 1653, 2000, 2455, 2759
AlwNI	CAGNNNCTG	1 cut(s)	2009
Ama87I	CYCGRG	4 cut(s)	1318, 1641, 2358, 2627
Aor13HI	TCCGGA	1 cut(s)	97
AoxI	GGCC	4 cut(s)	1274, 1450, 2481, 2901
ApeKI	GCWGC	8 cut(s)	122, 295, 436, 719, 899, 1253, 1367, 2219
ApoI	RAATTY	3 cut(s)	1173, 2831, 2888
ArsI	GACNNNNNNTTYG	4 cut(s)	1977, 2009, 2706, 2738
AseI	ATTAAT	1 cut(s)	882
Asp700I	GAANNNNTTC	1 cut(s)	1668
Asp718I	GGTACC	1 cut(s)	2700
AspLEI	GCGC	4 cut(s)	122, 403, 421, 777
AsuC2I	CCSGG	2 cut(s)	2628, 2629
AsuHPI	GGTGA	8 cut(s)	146, 418, 1198, 1231, 1572, 1810, 1882, 2933
AvaI	CYCGRG	4 cut(s)	1318, 1641, 2358, 2627
AvaII	GGWCC	9 cut(s)	41, 1384, 1400, 1963, 2226, 2243, 2258, 2366, 2631
AxyI	CCTNAGG	1 cut(s)	1089
BaeGI	GKGCMC	1 cut(s)	432
BaeI	ACNNNNGTAYC	2 cut(s)	1668, 1701
BanI	GGYRCC	1 cut(s)	2700
BanII	GRGCYC	1 cut(s)	1977
BauI	CACGAG	1 cut(s)	2826
BbsI	GAAGAC	2 cut(s)	452, 1724
Bbv12I	GWGCWC	3 cut(s)	1917, 1977, 2359
BbvCI	CCTCAGC	1 cut(s)	204
BbvI	GCAGC	8 cut(s)	134, 307, 448, 706, 886, 1265, 1354, 2206
BccI	CCATC	9 cut(s)	689, 727, 972, 1453, 1837, 1895, 1961, 2393, 2902
BceAI	ACGGC	4 cut(s)	177, 1821, 2303, 2528
BcgI	CGANNNNNNTGC	6 cut(s)	963, 997, 1538, 1572, 2875, 2909
BciT130I	CCWGG	6 cut(s)	62, 645, 1184, 1674, 2760, 2822
BciVI	GTATCC	3 cut(s)	631, 1491, 1644
BclI	TGATCA	2 cut(s)	795, 2740
BcnI	CCSGG	2 cut(s)	2628, 2629
BcoDI	GTCTC	4 cut(s)	17, 251, 1733, 2681
BfaI	CTAG	5 cut(s)	1337, 2166, 2196, 2273, 2472
BfmI	CTRYAG	5 cut(s)	228, 720, 972, 1656, 2751
BfoI	RGCGCY	2 cut(s)	422, 778
BfuI	GTATCC	3 cut(s)	631, 1491, 1644
BisI	GCNGC	9 cut(s)	123, 287, 296, 437, 720, 900, 1254, 1368, 2220
BlpI	GCTNAGC	1 cut(s)	1227
BlsI	GCNGC	9 cut(s)	124, 288, 297, 438, 721, 901, 1255, 1369, 2221
Bme1390I	CCNGG	8 cut(s)	62, 645, 1184, 1674, 2628, 2629, 2760, 2822
Bme18I	GGWCC	9 cut(s)	41, 1384, 1400, 1963, 2226, 2243, 2258, 2366, 2631
BmeT110I	CYCGRG	4 cut(s)	1318, 1641, 2358, 2627
BmgBI	CACGTC	1 cut(s)	2477
BmiI	GGNNCC	8 cut(s)	43, 2259, 2260, 2368, 2523, 2632, 2633, 2702
BmrFI	CCNGG	8 cut(s)	62, 645, 1184, 1674, 2628, 2629, 2760, 2822
BmrI	ACTGGG	3 cut(s)	1574, 1787, 1821
BmuI	ACTGGG	3 cut(s)	1574, 1787, 1821
BoxI	GACNNNNGTC	1 cut(s)	263
BpiI	GAAGAC	2 cut(s)	452, 1724
BplI	GAGNNNNNCTC	4 cut(s)	1864, 1896, 1964, 1996
BpmI	CTGGAG	1 cut(s)	2781
Bpu10I	CCTNAGC	4 cut(s)	204, 860, 1244, 2430
Bpu1102I	GCTNAGC	1 cut(s)	1227
BpuEI	CTTGAG	4 cut(s)	311, 617, 2694, 2716
BpuMI	CCSGG	2 cut(s)	2628, 2629
Bsa29I	ATCGAT	1 cut(s)	2157
BsaJI	CCNNGG	4 cut(s)	839, 1317, 2510, 2627
BsaWI	WCCGGW	1 cut(s)	97
Bsc4I	CCNNNNNNNGG	6 cut(s)	403, 712, 944, 1124, 1459, 2627
Bse118I	RCCGGY	2 cut(s)	310, 2524
Bse1I	ACTGG	7 cut(s)	876, 1048, 1402, 1569, 1793, 1827, 2922
Bse21I	CCTNAGG	1 cut(s)	1089
Bse3DI	GCAATG	3 cut(s)	634, 989, 1255
BseAI	TCCGGA	1 cut(s)	97
BseBI	CCWGG	6 cut(s)	62, 645, 1184, 1674, 2760, 2822
BseCI	ATCGAT	1 cut(s)	2157
BseDI	CCNNGG	4 cut(s)	839, 1317, 2510, 2627
BseLI	CCNNNNNNNGG	6 cut(s)	403, 712, 944, 1124, 1459, 2627
BseMI	GCAATG	3 cut(s)	634, 989, 1255
BseNI	ACTGG	7 cut(s)	876, 1048, 1402, 1569, 1793, 1827, 2922
BseRI	GAGGAG	7 cut(s)	104, 248, 571, 1143, 1607, 1766, 1916
BseSI	GKGCMC	1 cut(s)	432
BseXI	GCAGC	8 cut(s)	134, 307, 448, 706, 886, 1265, 1354, 2206
BseYI	CCCAGC	1 cut(s)	2635
BsgI	GTGCAG	1 cut(s)	1166
BshFI	GGCC	4 cut(s)	1276, 1452, 2483, 2903
BshNI	GGYRCC	1 cut(s)	2700
BshVI	ATCGAT	1 cut(s)	2157
BsiHKAI	GWGCWC	3 cut(s)	1917, 1977, 2359
BsiHKCI	CYCGRG	4 cut(s)	1318, 1641, 2358, 2627
BsiSI	CCGG	4 cut(s)	98, 311, 2525, 2628
BslFI	GGGAC	3 cut(s)	27, 2271, 2644
BslI	CCNNNNNNNGG	6 cut(s)	403, 712, 944, 1124, 1459, 2627
BsmAI	GTCTC	4 cut(s)	17, 251, 1733, 2681
BsmBI	CGTCTC	1 cut(s)	2681
BsmFI	GGGAC	3 cut(s)	27, 2271, 2644
BsmI	GAATGC	1 cut(s)	2320
BsnI	GGCC	4 cut(s)	1276, 1452, 2483, 2903
BsoBI	CYCGRG	4 cut(s)	1318, 1641, 2358, 2627
Bsp1286I	GDGCHC	4 cut(s)	432, 1917, 1977, 2359
Bsp13I	TCCGGA	1 cut(s)	97
Bsp1407I	TGTACA	1 cut(s)	922
Bsp1720I	GCTNAGC	1 cut(s)	1227
BspACI	CCGC	3 cut(s)	287, 2126, 2805
BspANI	GGCC	4 cut(s)	1276, 1452, 2483, 2903
BspDI	ATCGAT	1 cut(s)	2157
BspEI	TCCGGA	1 cut(s)	97
BspHI	TCATGA	1 cut(s)	1956
BspLI	GGNNCC	8 cut(s)	43, 2259, 2260, 2368, 2523, 2632, 2633, 2702
BspMAI	CTGCAG	3 cut(s)	232, 724, 976
BspPI	GGATC	8 cut(s)	929, 1037, 1318, 1436, 1653, 2000, 2455, 2759
BspQI	GCTCTTC	1 cut(s)	1674
BspT107I	GGYRCC	1 cut(s)	2700
BsrDI	GCAATG	3 cut(s)	634, 989, 1255
BsrFI	RCCGGY	2 cut(s)	310, 2524
BsrGI	TGTACA	1 cut(s)	922
BsrI	ACTGG	7 cut(s)	876, 1048, 1402, 1569, 1793, 1827, 2922
BssAI	RCCGGY	2 cut(s)	310, 2524
BssECI	CCNNGG	4 cut(s)	839, 1317, 2510, 2627
BssNAI	GTATAC	1 cut(s)	528
BssSI	CACGAG	1 cut(s)	2826
BssT1I	CCWWGG	1 cut(s)	839
Bst1107I	GTATAC	1 cut(s)	528
Bst2BI	CACGAG	1 cut(s)	2826
Bst2UI	CCWGG	6 cut(s)	62, 645, 1184, 1674, 2760, 2822
Bst4CI	ACNGT	9 cut(s)	134, 372, 451, 1555, 1606, 2032, 2109, 2561, 2581
Bst6I	CTCTTC	3 cut(s)	355, 910, 1674
BstAPI	GCANNNNNTGC	3 cut(s)	1153, 1223, 2423
BstAUI	TGTACA	1 cut(s)	922
BstC8I	GCNNGC	3 cut(s)	201, 690, 2594
BstDSI	CCRYGG	1 cut(s)	2510
BstH2I	RGCGCY	2 cut(s)	422, 778
BstHHI	GCGC	4 cut(s)	122, 403, 421, 777
BstMAI	GTCTC	4 cut(s)	17, 251, 1733, 2681
BstNI	CCWGG	6 cut(s)	62, 645, 1184, 1674, 2760, 2822
BstNSI	RCATGY	2 cut(s)	1440, 2606
BstPAI	GACNNNNGTC	1 cut(s)	263
BstSCI	CCNGG	8 cut(s)	60, 643, 1182, 1672, 2626, 2627, 2758, 2820
BstSFI	CTRYAG	5 cut(s)	228, 720, 972, 1656, 2751
BstSLI	GKGCMC	1 cut(s)	432
BstV1I	GCAGC	8 cut(s)	134, 307, 448, 706, 886, 1265, 1354, 2206
BstV2I	GAAGAC	2 cut(s)	452, 1724
BstX2I	RGATCY	4 cut(s)	1310, 2005, 2447, 2764
BstXI	CCANNNNNNTGG	1 cut(s)	2911
BstYI	RGATCY	4 cut(s)	1310, 2005, 2447, 2764
BstZ17I	GTATAC	1 cut(s)	528
Bsu15I	ATCGAT	1 cut(s)	2157
Bsu36I	CCTNAGG	1 cut(s)	1089
BsuI	GTATCC	3 cut(s)	631, 1491, 1644
BsuRI	GGCC	4 cut(s)	1276, 1452, 2483, 2903
BsuTUI	ATCGAT	1 cut(s)	2157
BtgI	CCRYGG	1 cut(s)	2510
BtrI	CACGTC	1 cut(s)	2477
BtsIMutI	CAGTG	5 cut(s)	869, 1800, 1834, 2354, 2577
Cac8I	GCNNGC	3 cut(s)	201, 690, 2594
CaiI	CAGNNNCTG	1 cut(s)	2009
CciI	TCATGA	1 cut(s)	1956
CfoI	GCGC	4 cut(s)	122, 403, 421, 777
Cfr10I	RCCGGY	2 cut(s)	310, 2524
Cfr9I	CCCGGG	1 cut(s)	2627
ClaI	ATCGAT	1 cut(s)	2157
CseI	GACGC	1 cut(s)	1049
Csp6I	GTAC	8 cut(s)	619, 923, 1562, 1677, 1929, 1942, 2618, 2701
CspCI	CAANNNNNGTGG	4 cut(s)	1362, 1397, 1827, 1862
CviQI	GTAC	8 cut(s)	619, 923, 1562, 1677, 1929, 1942, 2618, 2701
DrdI	GACNNNNNNGTC	1 cut(s)	361
DseDI	GACNNNNNNGTC	1 cut(s)	361
Eam1104I	CTCTTC	3 cut(s)	355, 910, 1674
EarI	CTCTTC	3 cut(s)	355, 910, 1674
Ecl136II	GAGCTC	1 cut(s)	1975
Eco130I	CCWWGG	1 cut(s)	839
Eco147I	AGGCCT	1 cut(s)	1276
Eco24I	GRGCYC	1 cut(s)	1977
Eco32I	GATATC	1 cut(s)	564
Eco47I	GGWCC	9 cut(s)	41, 1384, 1400, 1963, 2226, 2243, 2258, 2366, 2631
Eco53kI	GAGCTC	1 cut(s)	1975
Eco57I	CTGAAG	2 cut(s)	1683, 1760
Eco81I	CCTNAGG	1 cut(s)	1089
Eco88I	CYCGRG	4 cut(s)	1318, 1641, 2358, 2627
EcoICRI	GAGCTC	1 cut(s)	1975
EcoO109I	RGGNCCY	5 cut(s)	41, 1963, 2258, 2366, 2631
EcoRII	CCWGG	6 cut(s)	60, 643, 1182, 1672, 2758, 2820
EcoRV	GATATC	1 cut(s)	564
EcoT14I	CCWWGG	1 cut(s)	839
EcoT22I	ATGCAT	1 cut(s)	1513
EcoT38I	GRGCYC	1 cut(s)	1977
ErhI	CCWWGG	1 cut(s)	839
Esp3I	CGTCTC	1 cut(s)	2681
FalI	AAGNNNNNCTT	4 cut(s)	605, 637, 832, 864
FaqI	GGGAC	3 cut(s)	27, 2271, 2644
FauI	CCCGC	1 cut(s)	2812
FauNDI	CATATG	2 cut(s)	486, 2231
FbaI	TGATCA	2 cut(s)	795, 2740
FblI	GTMKAC	3 cut(s)	177, 491, 527
Fnu4HI	GCNGC	9 cut(s)	123, 287, 296, 437, 720, 900, 1254, 1368, 2220
FriOI	GRGCYC	1 cut(s)	1977
Fsp4HI	GCNGC	9 cut(s)	123, 287, 296, 437, 720, 900, 1254, 1368, 2220
FspBI	CTAG	5 cut(s)	1337, 2166, 2196, 2273, 2472
GlaI	GCGC	4 cut(s)	121, 402, 420, 776
GluI	GCNGC	9 cut(s)	123, 287, 296, 437, 720, 900, 1254, 1368, 2220
GsaI	CCCAGC	1 cut(s)	2639
GsuI	CTGGAG	1 cut(s)	2781
HaeII	RGCGCY	2 cut(s)	422, 778
HaeIII	GGCC	4 cut(s)	1276, 1452, 2483, 2903
HapII	CCGG	4 cut(s)	98, 311, 2525, 2628
HgaI	GACGC	1 cut(s)	1049
HhaI	GCGC	4 cut(s)	122, 403, 421, 777
Hin6I	GCGC	4 cut(s)	120, 401, 419, 775
HinP1I	GCGC	4 cut(s)	120, 401, 419, 775
HincII	GTYRAC	2 cut(s)	808, 2299
HindII	GTYRAC	2 cut(s)	808, 2299
HindIII	AAGCTT	3 cut(s)	857, 1019, 2718
HpaII	CCGG	4 cut(s)	98, 311, 2525, 2628
HphI	GGTGA	8 cut(s)	146, 418, 1198, 1231, 1572, 1810, 1882, 2933
Hpy99I	CGWCG	1 cut(s)	134
HpyAV	CCTTC	6 cut(s)	719, 1362, 1565, 1688, 1954, 2357
HpyCH4III	ACNGT	9 cut(s)	134, 372, 451, 1555, 1606, 2032, 2109, 2561, 2581
HpyCH4IV	ACGT	2 cut(s)	174, 2476
HpySE526I	ACGT	2 cut(s)	174, 2476
HspAI	GCGC	4 cut(s)	120, 401, 419, 775
KflI	GGGWCCC	2 cut(s)	2258, 2631
Kpn2I	TCCGGA	1 cut(s)	97
KpnI	GGTACC	1 cut(s)	2704
Ksp22I	TGATCA	2 cut(s)	795, 2740
LguI	GCTCTTC	1 cut(s)	1674
LmnI	GCTCC	4 cut(s)	91, 148, 1912, 2521
Lsp1109I	GCAGC	8 cut(s)	134, 307, 448, 706, 886, 1265, 1354, 2206
MaeI	CTAG	5 cut(s)	1337, 2166, 2196, 2273, 2472
MaeII	ACGT	2 cut(s)	174, 2476
MaeIII	GTNAC	5 cut(s)	134, 1468, 1615, 1874, 2605
MfeI	CAATTG	1 cut(s)	783
MflI	RGATCY	4 cut(s)	1310, 2005, 2447, 2764
MhlI	GDGCHC	4 cut(s)	432, 1917, 1977, 2359
MlyI	GAGTC	5 cut(s)	62, 341, 1403, 1913, 2321
MmeI	TCCRAC	2 cut(s)	153, 2091
Mph1103I	ATGCAT	1 cut(s)	1513
MroI	TCCGGA	1 cut(s)	97
MroXI	GAANNNNTTC	1 cut(s)	1668
MseI	TTAA	8 cut(s)	324, 570, 882, 1017, 1464, 1608, 2397, 2649
MslI	CAYNNNNRTG	1 cut(s)	2909
MspA1I	CMGCKG	1 cut(s)	899
MspI	CCGG	4 cut(s)	98, 311, 2525, 2628
MspR9I	CCNGG	8 cut(s)	62, 645, 1184, 1674, 2628, 2629, 2760, 2822
MunI	CAATTG	1 cut(s)	783
Mva1269I	GAATGC	1 cut(s)	2320
MvaI	CCWGG	6 cut(s)	62, 645, 1184, 1674, 2760, 2822
NciI	CCSGG	2 cut(s)	2628, 2629
NdeI	CATATG	2 cut(s)	486, 2231
NlaIV	GGNNCC	8 cut(s)	43, 2259, 2260, 2368, 2523, 2632, 2633, 2702
NmuCI	GTSAC	1 cut(s)	134
NsiI	ATGCAT	1 cut(s)	1513
NspI	RCATGY	2 cut(s)	1440, 2606
PaeR7I	CTCGAG	1 cut(s)	2358
PagI	TCATGA	1 cut(s)	1956
PceI	AGGCCT	1 cut(s)	1276
PciI	ACATGT	2 cut(s)	1436, 2602
PciSI	GCTCTTC	1 cut(s)	1674
PctI	GAATGC	1 cut(s)	2320
PdmI	GAANNNNTTC	1 cut(s)	1668
PfeI	GAWTC	6 cut(s)	1537, 1822, 1949, 2039, 2672, 2812
PfoI	TCCNGGA	1 cut(s)	2758
PkrI	GCNGC	9 cut(s)	124, 288, 297, 438, 721, 901, 1255, 1369, 2221
PleI	GAGTC	5 cut(s)	61, 341, 1403, 1912, 2321
PpsI	GAGTC	5 cut(s)	61, 341, 1403, 1912, 2321
PpuMI	RGGWCCY	5 cut(s)	41, 1963, 2258, 2366, 2631
PscI	ACATGT	2 cut(s)	1436, 2602
PshAI	GACNNNNGTC	1 cut(s)	263
PshBI	ATTAAT	1 cut(s)	882
Psp124BI	GAGCTC	1 cut(s)	1977
Psp5II	RGGWCCY	5 cut(s)	41, 1963, 2258, 2366, 2631
Psp6I	CCWGG	6 cut(s)	60, 643, 1182, 1672, 2758, 2820
PspFI	CCCAGC	1 cut(s)	2635
PspGI	CCWGG	6 cut(s)	60, 643, 1182, 1672, 2758, 2820
PspN4I	GGNNCC	8 cut(s)	43, 2259, 2260, 2368, 2523, 2632, 2633, 2702
PspPPI	RGGWCCY	5 cut(s)	41, 1963, 2258, 2366, 2631
PstI	CTGCAG	3 cut(s)	232, 724, 976
PstNI	CAGNNNCTG	1 cut(s)	2009
PsuI	RGATCY	4 cut(s)	1310, 2005, 2447, 2764
PvuII	CAGCTG	1 cut(s)	899
RsaI	GTAC	8 cut(s)	620, 924, 1563, 1678, 1930, 1943, 2619, 2702
RsaNI	GTAC	8 cut(s)	619, 923, 1562, 1677, 1929, 1942, 2618, 2701
RseI	CAYNNNNRTG	1 cut(s)	2909
SacI	GAGCTC	1 cut(s)	1977
SapI	GCTCTTC	1 cut(s)	1674
SaqAI	TTAA	8 cut(s)	324, 570, 882, 1017, 1464, 1608, 2397, 2649
SatI	GCNGC	9 cut(s)	123, 287, 296, 437, 720, 900, 1254, 1368, 2220
SchI	GAGTC	5 cut(s)	62, 341, 1403, 1913, 2321
ScrFI	CCNGG	8 cut(s)	62, 645, 1184, 1674, 2628, 2629, 2760, 2822
SduI	GDGCHC	4 cut(s)	432, 1917, 1977, 2359
SfcI	CTRYAG	5 cut(s)	228, 720, 972, 1656, 2751
Sfr274I	CTCGAG	1 cut(s)	2358
SinI	GGWCC	9 cut(s)	41, 1384, 1400, 1963, 2226, 2243, 2258, 2366, 2631
SlaI	CTCGAG	1 cut(s)	2358
SmaI	CCCGGG	1 cut(s)	2629
SmiMI	CAYNNNNRTG	1 cut(s)	2909
SmlI	CTYRAG	5 cut(s)	290, 596, 2358, 2695, 2709
SmoI	CTYRAG	5 cut(s)	290, 596, 2358, 2695, 2709
SseBI	AGGCCT	1 cut(s)	1276
SsiI	CCGC	3 cut(s)	287, 2126, 2805
SspMI	CTAG	5 cut(s)	1337, 2166, 2196, 2273, 2472
SstI	GAGCTC	1 cut(s)	1977
StuI	AGGCCT	1 cut(s)	1276
StyD4I	CCNGG	8 cut(s)	60, 643, 1182, 1672, 2626, 2627, 2758, 2820
StyI	CCWWGG	1 cut(s)	839
TaaI	ACNGT	9 cut(s)	134, 372, 451, 1555, 1606, 2032, 2109, 2561, 2581
TaiI	ACGT	2 cut(s)	177, 2479
TaqI	TCGA	9 cut(s)	51, 353, 392, 1692, 2157, 2330, 2359, 2556, 2895
TatI	WGTACW	3 cut(s)	922, 1561, 1941
TauI	GCSGC	1 cut(s)	289
TfiI	GAWTC	6 cut(s)	1537, 1822, 1949, 2039, 2672, 2812
Tru1I	TTAA	8 cut(s)	324, 570, 882, 1017, 1464, 1608, 2397, 2649
Tru9I	TTAA	8 cut(s)	324, 570, 882, 1017, 1464, 1608, 2397, 2649
TscAI	CASTG	5 cut(s)	876, 1800, 1834, 2354, 2584
TseFI	GTSAC	1 cut(s)	134
TseI	GCWGC	8 cut(s)	122, 295, 436, 719, 899, 1253, 1367, 2219
Tsp45I	GTSAC	1 cut(s)	134
TspGWI	ACGGA	3 cut(s)	122, 1757, 2705
TspMI	CCCGGG	1 cut(s)	2627
TspRI	CASTG	5 cut(s)	876, 1800, 1834, 2354, 2584
VpaK11BI	GGWCC	9 cut(s)	41, 1384, 1400, 1963, 2226, 2243, 2258, 2366, 2631
VspI	ATTAAT	1 cut(s)	882
XapI	RAATTY	3 cut(s)	1173, 2831, 2888
XbaI	TCTAGA	1 cut(s)	2471
XceI	RCATGY	2 cut(s)	1440, 2606
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1394
XhoI	CTCGAG	1 cut(s)	2358
XmaI	CCCGGG	1 cut(s)	2627
XmiI	GTMKAC	3 cut(s)	177, 491, 527
XmnI	GAANNNNTTC	1 cut(s)	1668
XspI	CTAG	5 cut(s)	1337, 2166, 2196, 2273, 2472
Zsp2I	ATGCAT	1 cut(s)	1513

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.