Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0002643

Physical Location & Seq

Genomic Coordinates Reverse (-)

13071353 .. 13076990

Gene Structure 5638 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G006400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2907 bp

ATGGATGATAGTCTATACGACGAGTTCGGGAACTATATTGGACCCGAAATCGAGTCTGACCAGGAGAGTGATAGGGAGGAAGAAGATGAGGAGCTTCCGGATAGGTTAGAAGATAGAGGTGCGGCATCTGACGGTGAGGATGCTCAGAATGGGTGGATGACTGCCTCTAATGATGTAGATATGGACAACCAGGTTGTTCTTGCTGAGGATAAGAAGTATTATCCTACAGCGGAGGAGGTGTACGGTGAAGATGTCGAGACGTTGGTCATGGATGAAGATGCACAGCCCCTTGAACAGCTGATTATCAAGCCGGTTCGGAATATTAAGTTTGAGGTTGGTGTCAAGGACTCCTCGACGTATGTGTCGACGCAGTTTCTTGTTGGGCTTATGTCGAACCCTGCTCTGGTTTGGAATGTGGCGCTGGTGGGGCACTTGCAGCATGGGAAGACGGTGTTTATGGATATGTTGGTGGAACAGACTCATCATATGTCTACTTTTGATGCAAATAGTGATAAGCACATGAGGTACACTGATACGAGGATTGATGAACAAGAGAGGAGGATATCTATTAAGGCTGTTCCCATGTCCCTTGTACTTGAGGATAGCAATTCAAAGTCGTACCTGTGCAATGTCATGGATACTCCTGGTCATGTCAATTTCTCTGATGAAATGACTGTTTCACTTAGGCTCGCTGATGGTGCTGTCCTCATTGTTGATGCTGCAGAAGGGATGAGCATGGTTATAAAATTTTATGATTTTGATCAACAGGTAAATACTGAGAGGGCTATATGCCATGCAATCCAAGAGCGCCTACCAATTGTGGTTGTAATCAACAAGGTTGACAGGTTGATAACAGAACTTAAATTGCCCCCAAGGGATGCTTACTTCAAGCTTAGGCATACTCTGGAAGTGATTAATAAGCACATAACGGCTGCCTCTTCAACTGCTGGTCATGTACAAGTTATTGATCCTGTTGCTGGAAACGTATGCTTTGCAACTATATATGTTAAGCTTCATGGGATCGCTTTTGACGCGGATCAGTTTGCTTCTCATCTATGGGGAGATATGTGCTATCATCCTGACGACAGGGCTTTCAGGAGAAAACCCCCTGCAAGTGGAGGAGAAAGGTCGTTTGTCCAGTTTGTGCTTGAACCCCTGTATAAAATTTATAGCCAGGTGATTGGAGAACATAAAAAGAGTGTTGAGGCAACTCTTGCTGAACTTGGTGTCACCCTTAGCAATGCAGCTTACAAATTGAATGTTAGGCCTCTACTGAGGTTGGCTTGTAGCTCGGTTTTTGGATCTGCCTCGGGCTTCACTGATATGCTTGTGCAACATATTCCTTCTCCTAAAGATGCTGCAACAAGGAAAGTCGACCACACATATACTGGACCAAAAGACTCTTTGATTTACAAAGCCATGAAAACTTGTGATCCCAATGGCCCGTTGATGGTAAATGTTACCAAGCTGTATCCCAAGTCCGATTGTAGCGTGTTGGATGCATTTGGTAGGGTATACAGTGGCAAGATTCAGACAGGACAGTCAGTGCGAGTATTGGGAGAAGGGTATTCACCTGACGATGAGGAGGACATGACAGTCAAAGAAGTAATGAAGCTATGGCTTTACCACGCGCGAGATAGGATACCTATAGCTGAAGCTCCTCCTGGTACTTGGGTTCTCATCGAAGGGGTGGATGCTTCCATAATGAAAACTGCCACTCTGTGTAATGAGAGTTATGACGAGGATGTATACATATTCTGTCCTCTTCAGTTCAATACACTGCCAGTGGTGAAAACGGCAACTGAGCCTCTAAATCCCAGTGAGTTGCCAAAAATGGTGGAGGGTCTTAGAAAGATCTGCAAGAGTTATCCTCTTGCCATAACCAAAGTTGGGGAGTCTGGGGAGCACACAATCTTAGGTACAGGAGAACTGTATTTGGATTCCATCATGAAGGACCTCAGAGAGCTCTACTCTGAAGTAGAAGTCAAGGTTGCAGATCCTGTTGTTTCATTCTGCGAAACAGTTGTGGAATCTTCATCAATGAAGTGTTTTGCTGAAACACCAAACAGGAAGAATAAAATTACTATGATTGCAGAGCCATTAGAGAGAGGACTTGCGGAGGACATTGAGAAAGGTGTTGTAAGCATTGATTGGTCTAGGAGAGATATAGGTAATTTCTTCCAGACTAGGTATGAGTGGGATGTTCTTGCTGCACGGTCCATATGGGCATTCGGTCCTGATAAGCAGGGACCCAACATACTACTTGGTGACACACTTTCAAGCGAAGTTGGCAAGAGCTTGCTGAATGCTGTGAAAGACTCCATTGTGCAAGGGTTTCAGTGGGGTGCTCGAGAAGGACCTCTGTGTGATGAACCCATCAGAAATGTTAAGTTCAAGATTGTTGATGCAAGGATTGCACCTGAGCCGTTGCATCGTGGATCTGGTCAAATCATTCCCACATCTCGACGTGTGGCCTATTCATCTTTCCTAATGGCAACCCCACGGCTCATGGAGCCAGTATATTATGTGGAGGTAAATAATCCTCAAGTCCATACGACGATGAGATTTGCCGATGTTCCTCAACCCGGGACCCCAGCTTACATTGTTAAGGCATTTTTACCCGTCATAGAATCGTTTGGTTTTGAGACGGACTTGAGGTACCATACTCAAGGACAGGCTTTTTGTCTCTCAGTCTTTGATCATTGGGCTATAGTTCCTGGGGATCCTCTTGACAAGAGTATTGTTCTGCGACCACTTGAACCCGCACCGATTCAGCACTGGCTCGTGAATTTATGGTCAAGACAAGGCGGAATGAGCGAGGATGTGAGCATCAGCAAATTTTTTGATGAGGCCATGATGGTGGAACTGGCTCAGCAGGAAGCTGATATTCACCAGCAAATGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005730 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0007275 GO:0008150 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0010154 GO:0015030 GO:0016020 GO:0016604 GO:0016607 GO:0022414 GO:0030054 GO:0030312 GO:0030532 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043900 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045694 GO:0046540 GO:0048316 GO:0048367 GO:0048437 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051239 GO:0055044 GO:0061458 GO:0065007 GO:0070013 GO:0071007 GO:0071013 GO:0071944 GO:0090567 GO:0097525 GO:0097526 GO:0099402 GO:0120114 GO:1902494 GO:1990904 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

EFG_C EFG_IV EFTUD2 GTP_EFTU GTP_EFTU_D2

Protein Analysis

968

Amino Acids

108.39

Weight (kDa)

4.9

Isoelectric Point (pI)

37.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EFTUD2	PF16004	3 - 116	6.6e-35	116 kDa U5 small nuclear ribonucleoprotein component N-terminus
GTP_EFTU	PF00009	137 - 322	2.1e-36	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	501 - 575	8.5e-10	Elongation factor Tu domain 2
EFG_IV	PF03764	721 - 832	5.2e-19	Elongation factor G, domain IV
EFG_C	PF00679	855 - 912	7.6e-10	Elongation factor G C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002643)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06220 AT1G06220 AT1G06220 AT5G25230 AT5G25230
fragaria_vesca	FvH4_2g24581 FvH4_7g03790 FvH4_7g03790 FvH4_7g03790
malus_domestica	MD02G1013600.v1.1 MD15G1160400.v1.1
prunus_persica	Prupe.3G036300_v2.0.a1
pyrus_communis	pycom02g01260 pycom15g14420
rosa_chinensis	RchiOBHm_Chr1g0327101 RchiOBHm_Chr1g0327121 RchiOBHm_Chr2g0127751
rosa_laevigata	RLG00000012029 RLG00000012030 RLG00000030043
rosa_multiflora	Rmu_sc0000795.1_g000050 Rmu_sc0004240.1_g000007
rosa_roxburghii	Rroxscaffold_4G00323330 Rroxscaffold_6G00399840 Rroxscaffold_6G00400180
rosa_rugosa	Rorug01G0063500
rosa_samantha	Rh1AG080100 Rh1AG080300 Rh1AG080700 Rh1AG080900 Rh1BG064100 Rh1CG078700 Rh1CG078800 Rh1DG085100 Rh1DG085200 Rh2AG323400 Rh2BG332600 Rh2DG350900
rosa_wichuraiana	Rw1G006400 Rw2G026250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	743
AasI	GACNNNNNNGTC	3 cut(s)	361, 1540, 1595
Acc65I	GGTACC	1 cut(s)	2658
AccB1I	GGYRCC	1 cut(s)	2658
AccI	GTMKAC	5 cut(s)	365, 491, 1374, 1515, 1749
AccII	CGCG	3 cut(s)	1034, 1631, 1633
AccIII	TCCGGA	1 cut(s)	97
AciI	CCGC	6 cut(s)	122, 230, 1034, 2117, 2763, 2808
AclWI	GGATC	9 cut(s)	962, 1028, 1044, 1309, 1427, 1991, 2446, 2717, 2730
AcsI	RAATTY	4 cut(s)	746, 1164, 2788, 2837
AcuI	CTGAAG	3 cut(s)	1674, 1751, 1995
AdeI	CACNNNGTG	1 cut(s)	1722
AfaI	GTAC	8 cut(s)	242, 527, 594, 620, 957, 1669, 1921, 2660
AfiI	CCNNNNNNNGG	6 cut(s)	403, 977, 1115, 1450, 1890, 2585
AflIII	ACRYGT	1 cut(s)	2467
AjiI	CACGTC	1 cut(s)	2468
AjnI	CCWGG	6 cut(s)	60, 189, 643, 1173, 1663, 2716
Alw21I	GWGCWC	3 cut(s)	1908, 1968, 2350
Alw26I	GTCTC	3 cut(s)	251, 2639, 2690
AlwI	GGATC	9 cut(s)	962, 1028, 1044, 1309, 1427, 1991, 2446, 2717, 2730
AlwNI	CAGNNNCTG	1 cut(s)	2000
Ama87I	CYCGRG	3 cut(s)	1309, 2349, 2585
Aor13HI	TCCGGA	1 cut(s)	97
AoxI	GGCC	4 cut(s)	1265, 1441, 2472, 2850
ApeKI	GCWGC	6 cut(s)	436, 719, 932, 1244, 1358, 2210
ApoI	RAATTY	4 cut(s)	746, 1164, 2788, 2837
ArsI	GACNNNNNNTTYG	2 cut(s)	2664, 2696
AseI	ATTAAT	1 cut(s)	915
Asp718I	GGTACC	1 cut(s)	2658
AspLEI	GCGC	3 cut(s)	421, 810, 1633
AspS9I	GGNCC	9 cut(s)	41, 1391, 1442, 1954, 2217, 2234, 2249, 2357, 2589
AsuC2I	CCSGG	2 cut(s)	2586, 2587
AsuHPI	GGTGA	8 cut(s)	146, 257, 1189, 1222, 1563, 1801, 2279, 2882
AvaI	CYCGRG	3 cut(s)	1309, 2349, 2585
AvaII	GGWCC	8 cut(s)	41, 1391, 1954, 2217, 2234, 2249, 2357, 2589
BaeGI	GKGCMC	1 cut(s)	432
BaeI	ACNNNNGTAYC	2 cut(s)	1659, 1692
BamHI	GGATCC	1 cut(s)	2722
BanI	GGYRCC	1 cut(s)	2658
BanII	GRGCYC	1 cut(s)	1968
BauI	CACGAG	1 cut(s)	2783
BbsI	GAAGAC	1 cut(s)	452
Bbv12I	GWGCWC	3 cut(s)	1908, 1968, 2350
BbvCI	CCTCAGC	1 cut(s)	204
BbvI	GCAGC	6 cut(s)	448, 706, 919, 1256, 1345, 2197
BccI	CCATC	5 cut(s)	689, 1444, 1952, 2384, 2851
BceAI	ACGGC	4 cut(s)	945, 1812, 2410, 2519
BciT130I	CCWGG	6 cut(s)	62, 191, 645, 1175, 1665, 2718
BciVI	GTATCC	3 cut(s)	631, 1482, 1635
BclI	TGATCA	2 cut(s)	760, 2698
BcnI	CCSGG	2 cut(s)	2586, 2587
BcoDI	GTCTC	3 cut(s)	251, 2639, 2690
BfaI	CTAG	2 cut(s)	2157, 2187
BfmI	CTRYAG	4 cut(s)	225, 720, 1647, 2709
BfoI	RGCGCY	2 cut(s)	422, 811
BfuI	GTATCC	3 cut(s)	631, 1482, 1635
BglII	AGATCT	1 cut(s)	1854
BisI	GCNGC	7 cut(s)	123, 437, 720, 933, 1245, 1359, 2211
BlpI	GCTNAGC	1 cut(s)	2871
BlsI	GCNGC	7 cut(s)	124, 438, 721, 934, 1246, 1360, 2212
Bme1390I	CCNGG	8 cut(s)	62, 191, 645, 1175, 1665, 2586, 2587, 2718
Bme18I	GGWCC	8 cut(s)	41, 1391, 1954, 2217, 2234, 2249, 2357, 2589
BmeT110I	CYCGRG	3 cut(s)	1309, 2349, 2585
BmgBI	CACGTC	1 cut(s)	2468
BmgT120I	GGNCC	9 cut(s)	41, 1391, 1442, 1954, 2217, 2234, 2249, 2357, 2589
BmiI	GGNNCC	8 cut(s)	43, 2250, 2251, 2514, 2590, 2591, 2660, 2724
BmrFI	CCNGG	8 cut(s)	62, 191, 645, 1175, 1665, 2586, 2587, 2718
BmrI	ACTGGG	1 cut(s)	1812
BmuI	ACTGGG	1 cut(s)	1812
BoxI	GACNNNNGTC	1 cut(s)	263
BpiI	GAAGAC	1 cut(s)	452
BplI	GAGNNNNNCTC	4 cut(s)	1855, 1887, 1955, 1987
Bpu10I	CCTNAGC	4 cut(s)	204, 893, 1235, 2421
Bpu1102I	GCTNAGC	1 cut(s)	2871
BpuEI	CTTGAG	4 cut(s)	617, 2529, 2652, 2674
BpuMI	CCSGG	2 cut(s)	2586, 2587
BsaBI	GATNNNNATC	1 cut(s)	759
BsaJI	CCNNGG	5 cut(s)	872, 1308, 2501, 2585, 2717
BsaWI	WCCGGW	1 cut(s)	97
BsaXI	ACNNNNNCTCC	2 cut(s)	224, 254
Bsc4I	CCNNNNNNNGG	6 cut(s)	403, 977, 1115, 1450, 1890, 2585
Bse118I	RCCGGY	1 cut(s)	310
Bse1I	ACTGG	7 cut(s)	1138, 1393, 1784, 1818, 2516, 2783, 2871
Bse3DI	GCAATG	2 cut(s)	634, 1246
Bse8I	GATNNNNATC	1 cut(s)	759
BseAI	TCCGGA	1 cut(s)	97
BseBI	CCWGG	6 cut(s)	62, 191, 645, 1175, 1665, 2718
BseDI	CCNNGG	5 cut(s)	872, 1308, 2501, 2585, 2717
BseJI	GATNNNNATC	1 cut(s)	759
BseLI	CCNNNNNNNGG	6 cut(s)	403, 977, 1115, 1450, 1890, 2585
BseMI	GCAATG	2 cut(s)	634, 1246
BseMII	CTCAG	9 cut(s)	158, 195, 768, 1265, 1794, 1972, 2412, 2703, 2885
BseNI	ACTGG	7 cut(s)	1138, 1393, 1784, 1818, 2516, 2783, 2871
BseRI	GAGGAG	7 cut(s)	104, 248, 340, 571, 1134, 1598, 1650
BseSI	GKGCMC	1 cut(s)	432
BseXI	GCAGC	6 cut(s)	448, 706, 919, 1256, 1345, 2197
BseYI	CCCAGC	1 cut(s)	2593
BsgI	GTGCAG	1 cut(s)	2196
Bsh1236I	CGCG	3 cut(s)	1034, 1631, 1633
BshFI	GGCC	4 cut(s)	1267, 1443, 2474, 2852
BshNI	GGYRCC	1 cut(s)	2658
BsiHKAI	GWGCWC	3 cut(s)	1908, 1968, 2350
BsiHKCI	CYCGRG	3 cut(s)	1309, 2349, 2585
BsiSI	CCGG	3 cut(s)	98, 311, 2586
BslFI	GGGAC	3 cut(s)	571, 2262, 2602
BslI	CCNNNNNNNGG	6 cut(s)	403, 977, 1115, 1450, 1890, 2585
BsmAI	GTCTC	3 cut(s)	251, 2639, 2690
BsmBI	CGTCTC	2 cut(s)	251, 2639
BsmFI	GGGAC	3 cut(s)	571, 2262, 2602
BsmI	GAATGC	2 cut(s)	2228, 2311
BsnI	GGCC	4 cut(s)	1267, 1443, 2474, 2852
BsoBI	CYCGRG	3 cut(s)	1309, 2349, 2585
Bsp1286I	GDGCHC	4 cut(s)	432, 1908, 1968, 2350
Bsp13I	TCCGGA	1 cut(s)	97
Bsp1407I	TGTACA	1 cut(s)	955
Bsp1720I	GCTNAGC	1 cut(s)	2871
BspACI	CCGC	6 cut(s)	122, 230, 1034, 2117, 2763, 2808
BspANI	GGCC	4 cut(s)	1267, 1443, 2474, 2852
BspCNI	CTCAG	9 cut(s)	157, 196, 769, 1266, 1795, 1971, 2413, 2702, 2884
BspEI	TCCGGA	1 cut(s)	97
BspFNI	CGCG	3 cut(s)	1034, 1631, 1633
BspHI	TCATGA	1 cut(s)	1947
BspLI	GGNNCC	8 cut(s)	43, 2250, 2251, 2514, 2590, 2591, 2660, 2724
BspMAI	CTGCAG	1 cut(s)	724
BspPI	GGATC	9 cut(s)	962, 1028, 1044, 1309, 1427, 1991, 2446, 2717, 2730
BspT107I	GGYRCC	1 cut(s)	2658
BsrDI	GCAATG	2 cut(s)	634, 1246
BsrFI	RCCGGY	1 cut(s)	310
BsrGI	TGTACA	1 cut(s)	955
BsrI	ACTGG	7 cut(s)	1138, 1393, 1784, 1818, 2516, 2783, 2871
BssAI	RCCGGY	1 cut(s)	310
BssECI	CCNNGG	5 cut(s)	872, 1308, 2501, 2585, 2717
BssNAI	GTATAC	2 cut(s)	1516, 1750
BssSI	CACGAG	1 cut(s)	2783
BssT1I	CCWWGG	1 cut(s)	872
Bst1107I	GTATAC	2 cut(s)	1516, 1750
Bst2BI	CACGAG	1 cut(s)	2783
Bst2UI	CCWGG	6 cut(s)	62, 191, 645, 1175, 1665, 2718
Bst6I	CTCTTC	2 cut(s)	943, 1770
BstAPI	GCANNNNNTGC	2 cut(s)	1214, 2414
BstAUI	TGTACA	1 cut(s)	955
BstC8I	GCNNGC	2 cut(s)	690, 2300
BstDSI	CCRYGG	1 cut(s)	2501
BstFNI	CGCG	3 cut(s)	1034, 1631, 1633
BstH2I	RGCGCY	2 cut(s)	422, 811
BstHHI	GCGC	3 cut(s)	421, 810, 1633
BstMAI	GTCTC	3 cut(s)	251, 2639, 2690
BstMWI	GCNNNNNNNGC	9 cut(s)	427, 436, 698, 1031, 1214, 2289, 2414, 2512, 2814
BstNI	CCWGG	6 cut(s)	62, 191, 645, 1175, 1665, 2718
BstPAI	GACNNNNGTC	1 cut(s)	263
BstSCI	CCNGG	8 cut(s)	60, 189, 643, 1173, 1663, 2584, 2585, 2716
BstSFI	CTRYAG	4 cut(s)	225, 720, 1647, 2709
BstSLI	GKGCMC	1 cut(s)	432
BstUI	CGCG	3 cut(s)	1034, 1631, 1633
BstV1I	GCAGC	6 cut(s)	448, 706, 919, 1256, 1345, 2197
BstV2I	GAAGAC	1 cut(s)	452
BstX2I	RGATCY	5 cut(s)	1301, 1854, 1996, 2438, 2722
BstXI	CCANNNNNNTGG	2 cut(s)	1181, 2860
BstYI	RGATCY	5 cut(s)	1301, 1854, 1996, 2438, 2722
BstZ17I	GTATAC	2 cut(s)	1516, 1750
BsuI	GTATCC	3 cut(s)	631, 1482, 1635
BsuRI	GGCC	4 cut(s)	1267, 1443, 2474, 2852
BtgI	CCRYGG	1 cut(s)	2501
BtrI	CACGTC	1 cut(s)	2468
BtsI	GCAGTG	1 cut(s)	1778
BtsIMutI	CAGTG	9 cut(s)	528, 1317, 1525, 1551, 1778, 1791, 1825, 2345, 2776
Cac8I	GCNNGC	2 cut(s)	690, 2300
CaiI	CAGNNNCTG	1 cut(s)	2000
CciI	TCATGA	1 cut(s)	1947
CfoI	GCGC	3 cut(s)	421, 810, 1633
Cfr10I	RCCGGY	1 cut(s)	310
Cfr13I	GGNCC	9 cut(s)	41, 1391, 1442, 1954, 2217, 2234, 2249, 2357, 2589
Cfr9I	CCCGGG	1 cut(s)	2585
CseI	GACGC	2 cut(s)	376, 1040
CsiI	ACCWGGT	1 cut(s)	189
Csp6I	GTAC	8 cut(s)	241, 526, 593, 619, 956, 1668, 1920, 2659
CspCI	CAANNNNNGTGG	2 cut(s)	1818, 1853
CviQI	GTAC	8 cut(s)	241, 526, 593, 619, 956, 1668, 1920, 2659
DraIII	CACNNNGTG	1 cut(s)	1722
DrdI	GACNNNNNNGTC	3 cut(s)	361, 1540, 1595
DseDI	GACNNNNNNGTC	3 cut(s)	361, 1540, 1595
Eam1104I	CTCTTC	2 cut(s)	943, 1770
EarI	CTCTTC	2 cut(s)	943, 1770
EciI	GGCGGA	1 cut(s)	2823
Ecl136II	GAGCTC	1 cut(s)	1966
Eco130I	CCWWGG	1 cut(s)	872
Eco147I	AGGCCT	1 cut(s)	1267
Eco24I	GRGCYC	1 cut(s)	1968
Eco32I	GATATC	1 cut(s)	564
Eco47I	GGWCC	8 cut(s)	41, 1391, 1954, 2217, 2234, 2249, 2357, 2589
Eco53kI	GAGCTC	1 cut(s)	1966
Eco57I	CTGAAG	3 cut(s)	1674, 1751, 1995
Eco88I	CYCGRG	3 cut(s)	1309, 2349, 2585
EcoICRI	GAGCTC	1 cut(s)	1966
EcoO109I	RGGNCCY	4 cut(s)	1954, 2249, 2357, 2589
EcoRII	CCWGG	6 cut(s)	60, 189, 643, 1173, 1663, 2716
EcoRV	GATATC	1 cut(s)	564
EcoT14I	CCWWGG	1 cut(s)	872
EcoT22I	ATGCAT	1 cut(s)	1504
EcoT38I	GRGCYC	1 cut(s)	1968
ErhI	CCWWGG	1 cut(s)	872
Esp3I	CGTCTC	2 cut(s)	251, 2639
FalI	AAGNNNNNCTT	2 cut(s)	865, 897
FaqI	GGGAC	3 cut(s)	571, 2262, 2602
FauI	CCCGC	1 cut(s)	2770
FauNDI	CATATG	2 cut(s)	486, 2222
FbaI	TGATCA	2 cut(s)	760, 2698
FblI	GTMKAC	5 cut(s)	365, 491, 1374, 1515, 1749
Fnu4HI	GCNGC	7 cut(s)	123, 437, 720, 933, 1245, 1359, 2211
FriOI	GRGCYC	1 cut(s)	1968
Fsp4HI	GCNGC	7 cut(s)	123, 437, 720, 933, 1245, 1359, 2211
FspBI	CTAG	2 cut(s)	2157, 2187
GlaI	GCGC	3 cut(s)	420, 809, 1632
GluI	GCNGC	7 cut(s)	123, 437, 720, 933, 1245, 1359, 2211
GsaI	CCCAGC	1 cut(s)	2597
HaeII	RGCGCY	2 cut(s)	422, 811
HaeIII	GGCC	4 cut(s)	1267, 1443, 2474, 2852
HapII	CCGG	3 cut(s)	98, 311, 2586
HgaI	GACGC	2 cut(s)	376, 1040
HhaI	GCGC	3 cut(s)	421, 810, 1633
Hin6I	GCGC	3 cut(s)	419, 808, 1631
HinP1I	GCGC	3 cut(s)	419, 808, 1631
HincII	GTYRAC	3 cut(s)	366, 841, 1375
HindII	GTYRAC	3 cut(s)	366, 841, 1375
HindIII	AAGCTT	2 cut(s)	890, 1010
HpaII	CCGG	3 cut(s)	98, 311, 2586
HphI	GGTGA	8 cut(s)	146, 257, 1189, 1222, 1563, 1801, 2279, 2882
Hpy166II	GTNNAC	8 cut(s)	241, 366, 492, 528, 841, 1375, 1516, 1750
Hpy8I	GTNNAC	8 cut(s)	241, 366, 492, 528, 841, 1375, 1516, 1750
Hpy99I	CGWCG	5 cut(s)	23, 358, 370, 2469, 2560
HpyAV	CCTTC	6 cut(s)	719, 1353, 1556, 1679, 1945, 2348
HpyCH4IV	ACGT	4 cut(s)	260, 356, 984, 2467
HpyF10VI	GCNNNNNNNGC	9 cut(s)	427, 436, 698, 1031, 1214, 2289, 2414, 2512, 2814
HpySE526I	ACGT	4 cut(s)	260, 356, 984, 2467
HspAI	GCGC	3 cut(s)	419, 808, 1631
KflI	GGGWCCC	2 cut(s)	2249, 2589
Kpn2I	TCCGGA	1 cut(s)	97
KpnI	GGTACC	1 cut(s)	2662
Ksp22I	TGATCA	2 cut(s)	760, 2698
LmnI	GCTCC	4 cut(s)	91, 1663, 1903, 2512
Lsp1109I	GCAGC	6 cut(s)	448, 706, 919, 1256, 1345, 2197
MabI	ACCWGGT	1 cut(s)	189
MaeI	CTAG	2 cut(s)	2157, 2187
MaeII	ACGT	4 cut(s)	260, 356, 984, 2467
MaeIII	GTNAC	3 cut(s)	1228, 1459, 2267
MfeI	CAATTG	1 cut(s)	816
MflI	RGATCY	5 cut(s)	1301, 1854, 1996, 2438, 2722
MhlI	GDGCHC	4 cut(s)	432, 1908, 1968, 2350
MlyI	GAGTC	6 cut(s)	62, 341, 472, 1394, 1904, 2312
MmeI	TCCRAC	1 cut(s)	1476
Mph1103I	ATGCAT	1 cut(s)	1504
MroI	TCCGGA	1 cut(s)	97
MseI	TTAA	7 cut(s)	324, 570, 861, 915, 1008, 2388, 2607
MslI	CAYNNNNRTG	2 cut(s)	1322, 2858
MspA1I	CMGCKG	2 cut(s)	230, 298
MspI	CCGG	3 cut(s)	98, 311, 2586
MspR9I	CCNGG	8 cut(s)	62, 191, 645, 1175, 1665, 2586, 2587, 2718
MunI	CAATTG	1 cut(s)	816
Mva1269I	GAATGC	2 cut(s)	2228, 2311
MvaI	CCWGG	6 cut(s)	62, 191, 645, 1175, 1665, 2718
MvnI	CGCG	3 cut(s)	1034, 1631, 1633
MwoI	GCNNNNNNNGC	9 cut(s)	427, 436, 698, 1031, 1214, 2289, 2414, 2512, 2814
NciI	CCSGG	2 cut(s)	2586, 2587
NdeI	CATATG	2 cut(s)	486, 2222
NlaIV	GGNNCC	8 cut(s)	43, 2250, 2251, 2514, 2590, 2591, 2660, 2724
NmuCI	GTSAC	2 cut(s)	1228, 2267
NsiI	ATGCAT	1 cut(s)	1504
PaeR7I	CTCGAG	1 cut(s)	2349
PagI	TCATGA	1 cut(s)	1947
PceI	AGGCCT	1 cut(s)	1267
PcsI	WCGNNNNNNNCGW	1 cut(s)	362
PctI	GAATGC	2 cut(s)	2228, 2311
PfeI	GAWTC	5 cut(s)	1528, 1940, 2030, 2630, 2770
PkrI	GCNGC	7 cut(s)	124, 438, 721, 934, 1246, 1360, 2212
PleI	GAGTC	6 cut(s)	61, 341, 472, 1394, 1903, 2312
PpsI	GAGTC	6 cut(s)	61, 341, 472, 1394, 1903, 2312
PpuMI	RGGWCCY	4 cut(s)	1954, 2249, 2357, 2589
PshAI	GACNNNNGTC	1 cut(s)	263
PshBI	ATTAAT	1 cut(s)	915
PsiI	TTATAA	1 cut(s)	743
Psp124BI	GAGCTC	1 cut(s)	1968
Psp5II	RGGWCCY	4 cut(s)	1954, 2249, 2357, 2589
Psp6I	CCWGG	6 cut(s)	60, 189, 643, 1173, 1663, 2716
PspFI	CCCAGC	1 cut(s)	2593
PspGI	CCWGG	6 cut(s)	60, 189, 643, 1173, 1663, 2716
PspN4I	GGNNCC	8 cut(s)	43, 2250, 2251, 2514, 2590, 2591, 2660, 2724
PspPI	GGNCC	9 cut(s)	41, 1391, 1442, 1954, 2217, 2234, 2249, 2357, 2589
PspPPI	RGGWCCY	4 cut(s)	1954, 2249, 2357, 2589
PstI	CTGCAG	1 cut(s)	724
PstNI	CAGNNNCTG	1 cut(s)	2000
PsuI	RGATCY	5 cut(s)	1301, 1854, 1996, 2438, 2722
PvuII	CAGCTG	1 cut(s)	298
RsaI	GTAC	8 cut(s)	242, 527, 594, 620, 957, 1669, 1921, 2660
RsaNI	GTAC	8 cut(s)	241, 526, 593, 619, 956, 1668, 1920, 2659
RseI	CAYNNNNRTG	2 cut(s)	1322, 2858
SacI	GAGCTC	1 cut(s)	1968
SalI	GTCGAC	2 cut(s)	364, 1373
SaqAI	TTAA	7 cut(s)	324, 570, 861, 915, 1008, 2388, 2607
SatI	GCNGC	7 cut(s)	123, 437, 720, 933, 1245, 1359, 2211
Sau96I	GGNCC	9 cut(s)	41, 1391, 1442, 1954, 2217, 2234, 2249, 2357, 2589
SchI	GAGTC	6 cut(s)	62, 341, 472, 1394, 1904, 2312
ScrFI	CCNGG	8 cut(s)	62, 191, 645, 1175, 1665, 2586, 2587, 2718
SduI	GDGCHC	4 cut(s)	432, 1908, 1968, 2350
SexAI	ACCWGGT	1 cut(s)	189
SfcI	CTRYAG	4 cut(s)	225, 720, 1647, 2709
Sfr274I	CTCGAG	1 cut(s)	2349
SinI	GGWCC	8 cut(s)	41, 1391, 1954, 2217, 2234, 2249, 2357, 2589
SlaI	CTCGAG	1 cut(s)	2349
SmaI	CCCGGG	1 cut(s)	2587
SmiMI	CAYNNNNRTG	2 cut(s)	1322, 2858
SmlI	CTYRAG	5 cut(s)	596, 2349, 2544, 2653, 2667
SmoI	CTYRAG	5 cut(s)	596, 2349, 2544, 2653, 2667
SseBI	AGGCCT	1 cut(s)	1267
SsiI	CCGC	6 cut(s)	122, 230, 1034, 2117, 2763, 2808
SspI	AATATT	1 cut(s)	322
SspMI	CTAG	2 cut(s)	2157, 2187
SstI	GAGCTC	1 cut(s)	1968
StuI	AGGCCT	1 cut(s)	1267
StyD4I	CCNGG	8 cut(s)	60, 189, 643, 1173, 1663, 2584, 2585, 2716
StyI	CCWWGG	1 cut(s)	872
TaiI	ACGT	4 cut(s)	263, 359, 987, 2470
TaqI	TCGA	9 cut(s)	51, 255, 353, 365, 392, 1374, 1683, 2350, 2464
TaqII	GACCGA	1 cut(s)	2222
TatI	WGTACW	2 cut(s)	592, 955
TauI	GCSGC	1 cut(s)	125
TfiI	GAWTC	5 cut(s)	1528, 1940, 2030, 2630, 2770
Tru1I	TTAA	7 cut(s)	324, 570, 861, 915, 1008, 2388, 2607
Tru9I	TTAA	7 cut(s)	324, 570, 861, 915, 1008, 2388, 2607
TscAI	CASTG	9 cut(s)	535, 1324, 1525, 1551, 1785, 1791, 1825, 2345, 2783
TseFI	GTSAC	2 cut(s)	1228, 2267
TseI	GCWGC	6 cut(s)	436, 719, 932, 1244, 1358, 2210
Tsp45I	GTSAC	2 cut(s)	1228, 2267
TspGWI	ACGGA	1 cut(s)	2663
TspMI	CCCGGG	1 cut(s)	2585
TspRI	CASTG	9 cut(s)	535, 1324, 1525, 1551, 1785, 1791, 1825, 2345, 2783
VpaK11BI	GGWCC	8 cut(s)	41, 1391, 1954, 2217, 2234, 2249, 2357, 2589
VspI	ATTAAT	1 cut(s)	915
XapI	RAATTY	4 cut(s)	746, 1164, 2788, 2837
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1385
XhoI	CTCGAG	1 cut(s)	2349
XmaI	CCCGGG	1 cut(s)	2585
XmiI	GTMKAC	5 cut(s)	365, 491, 1374, 1515, 1749
XspI	CTAG	2 cut(s)	2157, 2187
Zsp2I	ATGCAT	1 cut(s)	1504

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.