AT1G10220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003633
Physical Location & Seq
Forward (+)
3351923 .. 3353122
1200 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G10220.1

Sequence Viewer

Length: 624 bp
ATGTTGAGTCCGTTTTCGTCACCTAGGAGATCGAGAAGAGGAAACAAAGAAAGCAAGAATCCTTATTCAAATCAAGGACTTGACAAGTTCTCTGCACTTCTATCTGAGCTCGATGAGAAAAGACAGAGCATTTACGCAAAGAGGCTTGATCCTGATGGACCGCCTCTTGTTCGGTTTGTCTTCACTAGCTCCGGCGAGTGCGTCCCTGTAATGATCAAGACAAAGAGGGCAAGTCAGAAGAAGGATGTTCTAGATGATTTCAAGGTCAAGAATAAGGATGTTCTAGATGATTTCAATGTAAAGACCGAATCAAAAACAGAGCAGGAAAAAGAAATAAAGCAAACAGATTTGGAAACAGAGCAGAAACAGAGCTGTGTATTGAATGAGAATCTGAAGAAGATTTCAAGACCAAACCATTTATTACCCGTGACAGTGGTGCTGGTTCTAATATTATTGGTGTTTTTCGGACGGACCGTCTCGATCATGTGCACTTGTATCGTTTGGTACTTGGTTCCAATGATCAAGGAACAGAGCAGAAACAGAGGATCAACATACGAGACGAAGAGGAGGAAGAAACTAAATATTGAAAACAGAGCTGCTCCTGATAAAAGTGAAACCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

207

Amino Acids

23.88

Weight (kDa)

9.65

Isoelectric Point (pI)

69.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 161
AclWI GGATC 2 cut(s) 143, 553
AcuI CTGAAG 1 cut(s) 413
AfaI GTAC 1 cut(s) 506
AgsI TTSAA 6 cut(s) 69, 262, 295, 382, 405, 587
AhdI GACNNNNNGTC 1 cut(s) 473
AluBI AGCT 4 cut(s) 109, 189, 372, 596
AluI AGCT 4 cut(s) 109, 189, 372, 596
Alw21I GWGCWC 2 cut(s) 111, 491
Alw26I GTCTC 2 cut(s) 481, 551
Alw44I GTGCAC 1 cut(s) 487
AlwI GGATC 2 cut(s) 143, 553
ApaLI GTGCAC 1 cut(s) 487
ApeKI GCWGC 1 cut(s) 596
AspA2I CCTAGG 1 cut(s) 23
AspS9I GGNCC 2 cut(s) 158, 471
AsuC2I CCSGG 1 cut(s) 619
AsuHPI GGTGA 1 cut(s) 12
AvaII GGWCC 2 cut(s) 158, 471
AvrII CCTAGG 1 cut(s) 23
BaeGI GKGCMC 1 cut(s) 491
BanII GRGCYC 1 cut(s) 111
BbsI GAAGAC 1 cut(s) 172
Bbv12I GWGCWC 2 cut(s) 111, 491
BbvI GCAGC 1 cut(s) 583
BccI CCATC 1 cut(s) 149
BcgI CGANNNNNNTGC 2 cut(s) 478, 512
BclI TGATCA 2 cut(s) 213, 519
BcnI CCSGG 1 cut(s) 619
BcoDI GTCTC 2 cut(s) 481, 551
BfaI CTAG 4 cut(s) 24, 186, 251, 284
BisI GCNGC 1 cut(s) 597
BlnI CCTAGG 1 cut(s) 23
BlsI GCNGC 1 cut(s) 598
Bme1390I CCNGG 1 cut(s) 619
Bme18I GGWCC 2 cut(s) 158, 471
BmeRI GACNNNNNGTC 1 cut(s) 473
BmgT120I GGNCC 2 cut(s) 158, 471
BmiI GGNNCC 1 cut(s) 513
BmrFI CCNGG 1 cut(s) 619
BpiI GAAGAC 1 cut(s) 172
BpuMI CCSGG 1 cut(s) 619
BsaJI CCNNGG 1 cut(s) 23
BseDI CCNNGG 1 cut(s) 23
BseGI GGATG 2 cut(s) 250, 283
BseMII CTCAG 1 cut(s) 96
BseRI GAGGAG 1 cut(s) 580
BseSI GKGCMC 1 cut(s) 491
BseXI GCAGC 1 cut(s) 583
BsgI GTGCAG 1 cut(s) 78
BsiHKAI GWGCWC 2 cut(s) 111, 491
BsiSI CCGG 2 cut(s) 192, 619
BslFI GGGAC 1 cut(s) 188
BsmAI GTCTC 2 cut(s) 481, 551
BsmBI CGTCTC 2 cut(s) 481, 551
BsmFI GGGAC 1 cut(s) 188
Bsp1286I GDGCHC 2 cut(s) 111, 491
Bsp143I GATC 6 cut(s) 29, 148, 213, 480, 519, 545
BspACI CCGC 1 cut(s) 161
BspCNI CTCAG 1 cut(s) 97
BspLI GGNNCC 1 cut(s) 513
BspPI GGATC 2 cut(s) 143, 553
BssECI CCNNGG 1 cut(s) 23
BssMI GATC 6 cut(s) 29, 148, 213, 480, 519, 545
BssT1I CCWWGG 1 cut(s) 23
Bst4CI ACNGT 2 cut(s) 433, 475
Bst6I CTCTTC 2 cut(s) 31, 557
BstDEI CTNAG 1 cut(s) 105
BstF5I GGATG 2 cut(s) 250, 283
BstKTI GATC 6 cut(s) 32, 151, 216, 483, 522, 548
BstMAI GTCTC 2 cut(s) 481, 551
BstMBI GATC 6 cut(s) 29, 148, 213, 480, 519, 545
BstSCI CCNGG 1 cut(s) 617
BstSLI GKGCMC 1 cut(s) 491
BstV1I GCAGC 1 cut(s) 583
BstV2I GAAGAC 1 cut(s) 172
BtsCI GGATG 2 cut(s) 250, 283
BtsIMutI CAGTG 1 cut(s) 438
Cfr13I GGNCC 2 cut(s) 158, 471
CpoI CGGWCCG 1 cut(s) 471
CseI GACGC 1 cut(s) 190
Csp6I GTAC 1 cut(s) 505
CspI CGGWCCG 1 cut(s) 471
CviAII CATG 1 cut(s) 484
CviJI RGCY 5 cut(s) 109, 145, 189, 372, 596
CviKI_1 RGCY 5 cut(s) 109, 145, 189, 372, 596
CviQI GTAC 1 cut(s) 505
DdeI CTNAG 1 cut(s) 105
DpnI GATC 6 cut(s) 31, 150, 215, 482, 521, 547
DpnII GATC 6 cut(s) 29, 148, 213, 480, 519, 545
DriI GACNNNNNGTC 1 cut(s) 473
Eam1104I CTCTTC 2 cut(s) 31, 557
Eam1105I GACNNNNNGTC 1 cut(s) 473
EarI CTCTTC 2 cut(s) 31, 557
Ecl136II GAGCTC 1 cut(s) 109
Eco130I CCWWGG 1 cut(s) 23
Eco24I GRGCYC 1 cut(s) 111
Eco47I GGWCC 2 cut(s) 158, 471
Eco53kI GAGCTC 1 cut(s) 109
Eco57I CTGAAG 1 cut(s) 413
EcoICRI GAGCTC 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 23
EcoT38I GRGCYC 1 cut(s) 111
ErhI CCWWGG 1 cut(s) 23
Esp3I CGTCTC 2 cut(s) 481, 551
FaeI CATG 1 cut(s) 487
FaiI YATR 2 cut(s) 485, 553
FaqI GGGAC 1 cut(s) 188
FatI CATG 1 cut(s) 483
FbaI TGATCA 2 cut(s) 213, 519
Fnu4HI GCNGC 1 cut(s) 597
FokI GGATG 2 cut(s) 257, 290
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 1 cut(s) 597
FspBI CTAG 4 cut(s) 24, 186, 251, 284
GluI GCNGC 1 cut(s) 597
HapII CCGG 2 cut(s) 192, 619
HgaI GACGC 1 cut(s) 190
Hin1II CATG 1 cut(s) 487
HinfI GANTC 4 cut(s) 7, 58, 308, 388
HpaII CCGG 2 cut(s) 192, 619
HphI GGTGA 1 cut(s) 12
Hpy166II GTNNAC 1 cut(s) 489
Hpy188I TCNGA 4 cut(s) 106, 237, 393, 467
Hpy188III TCNNGA 9 cut(s) 33, 152, 217, 251, 268, 284, 405, 478, 602
Hpy8I GTNNAC 1 cut(s) 489
HpyAV CCTTC 1 cut(s) 235
HpyCH4III ACNGT 2 cut(s) 433, 475
HpyCH4V TGCA 2 cut(s) 95, 489
HpyF3I CTNAG 1 cut(s) 105
Hsp92II CATG 1 cut(s) 487
Ksp22I TGATCA 2 cut(s) 213, 519
Kzo9I GATC 6 cut(s) 29, 148, 213, 480, 519, 545
LmnI GCTCC 2 cut(s) 194, 604
LpnPI CCDG 6 cut(s) 165, 205, 219, 308, 425, 615
Lsp1109I GCAGC 1 cut(s) 583
MaeI CTAG 4 cut(s) 24, 186, 251, 284
MaeIII GTNAC 2 cut(s) 18, 427
MalI GATC 6 cut(s) 31, 150, 215, 482, 521, 547
MboI GATC 6 cut(s) 29, 148, 213, 480, 519, 545
MboII GAAGA 7 cut(s) 48, 172, 250, 406, 409, 574, 583
MhlI GDGCHC 2 cut(s) 111, 491
MlyI GAGTC 1 cut(s) 16
MnlI CCTC 7 cut(s) 32, 135, 174, 219, 536, 558, 561
MspI CCGG 2 cut(s) 192, 619
MspR9I CCNGG 1 cut(s) 619
NciI CCSGG 1 cut(s) 619
NdeII GATC 6 cut(s) 29, 148, 213, 480, 519, 545
NlaIII CATG 1 cut(s) 487
NlaIV GGNNCC 1 cut(s) 513
NmuCI GTSAC 2 cut(s) 18, 427
PcsI WCGNNNNNNNCGW 1 cut(s) 471
PfeI GAWTC 3 cut(s) 58, 308, 388
PkrI GCNGC 1 cut(s) 598
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
Psp124BI GAGCTC 1 cut(s) 111
PspN4I GGNNCC 1 cut(s) 513
PspPI GGNCC 2 cut(s) 158, 471
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
Rsr2I CGGWCCG 1 cut(s) 471
RsrII CGGWCCG 1 cut(s) 471
SacI GAGCTC 1 cut(s) 111
SatI GCNGC 1 cut(s) 597
Sau3AI GATC 6 cut(s) 29, 148, 213, 480, 519, 545
Sau96I GGNCC 2 cut(s) 158, 471
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 1 cut(s) 619
SduI GDGCHC 2 cut(s) 111, 491
SetI ASST 6 cut(s) 25, 111, 191, 267, 374, 598
SinI GGWCC 2 cut(s) 158, 471
SsiI CCGC 1 cut(s) 161
SspI AATATT 2 cut(s) 450, 583
SspMI CTAG 4 cut(s) 24, 186, 251, 284
SstI GAGCTC 1 cut(s) 111
StyD4I CCNGG 1 cut(s) 617
StyI CCWWGG 1 cut(s) 23
TaaI ACNGT 2 cut(s) 433, 475
TaqI TCGA 3 cut(s) 32, 111, 479
TaqII GACCGA 1 cut(s) 320
TfiI GAWTC 3 cut(s) 58, 308, 388
TscAI CASTG 1 cut(s) 438
TseFI GTSAC 2 cut(s) 18, 427
TseI GCWGC 1 cut(s) 596
Tsp45I GTSAC 2 cut(s) 18, 427
TspGWI ACGGA 1 cut(s) 484
TspRI CASTG 1 cut(s) 438
VneI GTGCAC 1 cut(s) 487
VpaK11BI GGWCC 2 cut(s) 158, 471
XbaI TCTAGA 2 cut(s) 250, 283
XmaJI CCTAGG 1 cut(s) 23
XspI CTAG 4 cut(s) 24, 186, 251, 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.