MD15G1068500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003633
Physical Location & Seq
Forward (+)
4734772 .. 4735572
801 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1068500.v1.1.491

Sequence Viewer

Length: 801 bp
ATGTTAAGCCCTTTCAGCTGCGGATTCTTCCACTCGGAAGACGTTCAAGATGAACCCATAAACGTTTCGAGTCCTTGCTCCACGCCGAGAAGATCAAAGAGAAGCAGCAAGGACAGCAAGAACCCCTATTCGAGTCGGGGTCTCGACAAGTTCTCTGCGCTCTTAGCCGATCTCGAAGAGAAGCGGCAGAAGATTTACGCGCAAACGCCTCCGCAAGACATATCCTTCGTCCGCTTCGTGTTCAAGGACTCGAAGGAGGACGCCCCTCCTATCCCGATCGTGATCAAGTTGAAGGACAACAAGAAAGAAGACCACAAAGAGAAAACCAAAACTCAGCTGGTGAAAGAAAAGCATGCAACGCGTAATTCGGGAAGTTTGGATAAGTTTACCGTTGAGCCCTCCACGCCCACAAAAGAAGTAGCAGCTCAGCAACCACCAAAGAATGATAAGAGGGTGCGTTCGTCATTGAACTTAAAGGGAATGAACTTGGGCATGTGGAGAAGGCCCTCTTACTATTTGCCGGTGGTTGTGGTGTTCATTTTGGTGCTGCTGGCAGTGTTCGGGCGGTCGTTTGCAATTCTGTGCACGTCTATCGGCTGGTACGCATTGCCGACGTTGAAAGAAAGCAATTCCGAGACAAAAAGAGCATCAAAGAAGAAAGACCATGGTAGAAGATTGAGTGACAATAATAATAATAATACTAATAATAATCATGGATCATTGTCTTCTCCAAAGAGTAACAACACGGCGGCCGACAGTACGCCCCGATCGCAACAACACGGTCACCGGAAAAGTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

267

Amino Acids

30.01

Weight (kDa)

9.91

Isoelectric Point (pI)

61.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 200, 361
AciI CCGC 6 cut(s) 21, 184, 212, 232, 565, 749
AclI AACGTT 1 cut(s) 63
AclWI GGATC 1 cut(s) 724
AcoI YGGCCR 1 cut(s) 750
AcyI GRCGYC 1 cut(s) 261
AfaI GTAC 2 cut(s) 602, 760
AflIII ACRYGT 1 cut(s) 359
AgsI TTSAA 5 cut(s) 47, 244, 292, 469, 619
AjiI CACGTC 1 cut(s) 588
AluBI AGCT 3 cut(s) 18, 337, 425
AluI AGCT 3 cut(s) 18, 337, 425
Alw21I GWGCWC 1 cut(s) 587
Alw26I GTCTC 2 cut(s) 146, 629
Alw44I GTGCAC 1 cut(s) 583
AlwI GGATC 1 cut(s) 724
AoxI GGCC 2 cut(s) 503, 750
ApaLI GTGCAC 1 cut(s) 583
ApeKI GCWGC 4 cut(s) 18, 105, 422, 547
ArsI GACNNNNNNTTYG 2 cut(s) 209, 241
Asp700I GAANNNNTTC 1 cut(s) 42
AspLEI GCGC 2 cut(s) 160, 202
AspS9I GGNCC 1 cut(s) 504
AsuHPI GGTGA 2 cut(s) 352, 776
BaeGI GKGCMC 1 cut(s) 587
BanII GRGCYC 1 cut(s) 399
BbsI GAAGAC 3 cut(s) 45, 315, 717
Bbv12I GWGCWC 1 cut(s) 587
BbvI GCAGC 4 cut(s) 5, 117, 434, 534
BceAI ACGGC 1 cut(s) 762
BcgI CGANNNNNNTGC 2 cut(s) 574, 608
BclI TGATCA 1 cut(s) 282
BcoDI GTCTC 2 cut(s) 146, 629
BisI GCNGC 6 cut(s) 19, 106, 185, 423, 548, 750
BlpI GCTNAGC 1 cut(s) 426
BlsI GCNGC 6 cut(s) 20, 107, 186, 424, 549, 751
BmgBI CACGTC 1 cut(s) 588
BmgT120I GGNCC 1 cut(s) 504
BmsI GCATC 1 cut(s) 656
BpiI GAAGAC 3 cut(s) 45, 315, 717
Bpu1102I GCTNAGC 1 cut(s) 426
BsaBI GATNNNNATC 1 cut(s) 281
BsaHI GRCGYC 1 cut(s) 261
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 1 cut(s) 664
BsaWI WCCGGW 1 cut(s) 786
Bse118I RCCGGY 1 cut(s) 520
Bse3DI GCAATG 1 cut(s) 605
Bse8I GATNNNNATC 1 cut(s) 281
BseDI CCNNGG 1 cut(s) 664
BseJI GATNNNNATC 1 cut(s) 281
BseMI GCAATG 1 cut(s) 605
BseMII CTCAG 2 cut(s) 347, 440
BseSI GKGCMC 1 cut(s) 587
BseX3I CGGCCG 1 cut(s) 750
BseXI GCAGC 4 cut(s) 5, 117, 434, 534
Bsh1236I CGCG 2 cut(s) 200, 361
Bsh1285I CGRYCG 4 cut(s) 279, 569, 753, 770
BshFI GGCC 2 cut(s) 505, 752
BsiEI CGRYCG 4 cut(s) 279, 569, 753, 770
BsiHKAI GWGCWC 1 cut(s) 587
BsiSI CCGG 2 cut(s) 521, 787
BsmAI GTCTC 2 cut(s) 146, 629
BsnI GGCC 2 cut(s) 505, 752
Bso31I GGTCTC 1 cut(s) 146
Bsp1286I GDGCHC 2 cut(s) 399, 587
Bsp143I GATC 6 cut(s) 92, 169, 276, 282, 716, 767
Bsp1720I GCTNAGC 1 cut(s) 426
Bsp19I CCATGG 1 cut(s) 664
BspACI CCGC 6 cut(s) 21, 184, 212, 232, 565, 749
BspANI GGCC 2 cut(s) 505, 752
BspCNI CTCAG 2 cut(s) 346, 439
BspFNI CGCG 2 cut(s) 200, 361
BspPI GGATC 1 cut(s) 724
BspTNI GGTCTC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 605
BsrFI RCCGGY 1 cut(s) 520
BssAI RCCGGY 1 cut(s) 520
BssECI CCNNGG 1 cut(s) 664
BssMI GATC 6 cut(s) 92, 169, 276, 282, 716, 767
BssNI GRCGYC 1 cut(s) 261
BssT1I CCWWGG 1 cut(s) 664
Bst4CI ACNGT 3 cut(s) 391, 758, 782
Bst6I CTCTTC 1 cut(s) 171
BstACI GRCGYC 1 cut(s) 261
BstC8I GCNNGC 2 cut(s) 354, 552
BstDEI CTNAG 3 cut(s) 163, 333, 426
BstDSI CCRYGG 1 cut(s) 664
BstEII GGTNACC 1 cut(s) 782
BstFNI CGCG 2 cut(s) 200, 361
BstHHI GCGC 2 cut(s) 160, 202
BstKTI GATC 6 cut(s) 95, 172, 279, 285, 719, 770
BstMAI GTCTC 2 cut(s) 146, 629
BstMBI GATC 6 cut(s) 92, 169, 276, 282, 716, 767
BstMCI CGRYCG 4 cut(s) 279, 569, 753, 770
BstMWI GCNNNNNNNGC 6 cut(s) 15, 114, 164, 358, 403, 769
BstNSI RCATGY 2 cut(s) 356, 496
BstPI GGTNACC 1 cut(s) 782
BstSLI GKGCMC 1 cut(s) 587
BstUI CGCG 2 cut(s) 200, 361
BstV1I GCAGC 4 cut(s) 5, 117, 434, 534
BstV2I GAAGAC 3 cut(s) 45, 315, 717
BstZI CGGCCG 1 cut(s) 750
BsuRI GGCC 2 cut(s) 505, 752
BtgI CCRYGG 1 cut(s) 664
BtrI CACGTC 1 cut(s) 588
BtsI GCAGTG 1 cut(s) 561
BtsIMutI CAGTG 1 cut(s) 561
Cac8I GCNNGC 2 cut(s) 354, 552
CfoI GCGC 2 cut(s) 160, 202
Cfr10I RCCGGY 1 cut(s) 520
Cfr13I GGNCC 1 cut(s) 504
CseI GACGC 1 cut(s) 269
Csp6I GTAC 2 cut(s) 601, 759
CviAII CATG 4 cut(s) 353, 493, 665, 713
CviJI RGCY 9 cut(s) 9, 18, 167, 337, 397, 425, 505, 597, 752
CviKI_1 RGCY 9 cut(s) 9, 18, 167, 337, 397, 425, 505, 597, 752
CviQI GTAC 2 cut(s) 601, 759
DdeI CTNAG 3 cut(s) 163, 333, 426
DpnI GATC 6 cut(s) 94, 171, 278, 284, 718, 769
DpnII GATC 6 cut(s) 92, 169, 276, 282, 716, 767
EaeI YGGCCR 1 cut(s) 750
EagI CGGCCG 1 cut(s) 750
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
EclXI CGGCCG 1 cut(s) 750
Eco130I CCWWGG 1 cut(s) 664
Eco24I GRGCYC 1 cut(s) 399
Eco31I GGTCTC 1 cut(s) 146
Eco52I CGGCCG 1 cut(s) 750
Eco91I GGTNACC 1 cut(s) 782
EcoO109I RGGNCCY 1 cut(s) 504
EcoO65I GGTNACC 1 cut(s) 782
EcoT14I CCWWGG 1 cut(s) 664
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 664
FaeI CATG 4 cut(s) 356, 496, 668, 716
FaiI YATR 6 cut(s) 59, 221, 354, 494, 666, 714
FalI AAGNNNNNCTT 2 cut(s) 493, 525
FatI CATG 4 cut(s) 352, 492, 664, 712
FbaI TGATCA 1 cut(s) 282
Fnu4HI GCNGC 6 cut(s) 19, 106, 185, 423, 548, 750
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 6 cut(s) 19, 106, 185, 423, 548, 750
GlaI GCGC 2 cut(s) 159, 201
GluI GCNGC 6 cut(s) 19, 106, 185, 423, 548, 750
HaeIII GGCC 2 cut(s) 505, 752
HapII CCGG 2 cut(s) 521, 787
HgaI GACGC 1 cut(s) 269
HhaI GCGC 2 cut(s) 160, 202
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 4 cut(s) 356, 496, 668, 716
Hin6I GCGC 2 cut(s) 158, 200
HinP1I GCGC 2 cut(s) 158, 200
HinfI GANTC 4 cut(s) 24, 70, 133, 248
HpaII CCGG 2 cut(s) 521, 787
HphI GGTGA 2 cut(s) 352, 776
Hpy166II GTNNAC 2 cut(s) 387, 585
Hpy188I TCNGA 2 cut(s) 37, 634
Hpy188III TCNNGA 6 cut(s) 47, 143, 173, 274, 280, 369
Hpy8I GTNNAC 2 cut(s) 387, 585
Hpy99I CGWCG 1 cut(s) 616
HpyAV CCTTC 4 cut(s) 235, 247, 286, 495
HpyCH4III ACNGT 3 cut(s) 391, 758, 782
HpyCH4IV ACGT 4 cut(s) 42, 63, 587, 614
HpyCH4V TGCA 3 cut(s) 356, 575, 585
HpyF10VI GCNNNNNNNGC 6 cut(s) 15, 114, 164, 358, 403, 769
HpyF3I CTNAG 3 cut(s) 163, 333, 426
HpySE526I ACGT 4 cut(s) 42, 63, 587, 614
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 4 cut(s) 356, 496, 668, 716
HspAI GCGC 2 cut(s) 158, 200
Ksp22I TGATCA 1 cut(s) 282
Kzo9I GATC 6 cut(s) 92, 169, 276, 282, 716, 767
LmnI GCTCC 1 cut(s) 83
LpnPI CCDG 4 cut(s) 323, 534, 536, 583
Lsp1109I GCAGC 4 cut(s) 5, 117, 434, 534
LweI GCATC 1 cut(s) 656
MaeII ACGT 4 cut(s) 42, 63, 587, 614
MaeIII GTNAC 3 cut(s) 680, 737, 782
MalI GATC 6 cut(s) 94, 171, 278, 284, 718, 769
MboI GATC 6 cut(s) 92, 169, 276, 282, 716, 767
MboII GAAGA 9 cut(s) 19, 50, 102, 188, 202, 320, 667, 684, 717
MhlI GDGCHC 2 cut(s) 399, 587
MluCI AATT 3 cut(s) 364, 576, 628
MluI ACGCGT 1 cut(s) 359
MlyI GAGTC 3 cut(s) 79, 142, 242
MnlI CCTC 6 cut(s) 219, 250, 276, 409, 444, 517
MroXI GAANNNNTTC 1 cut(s) 42
MseI TTAA 2 cut(s) 5, 473
MslI CAYNNNNRTG 1 cut(s) 542
MspA1I CMGCKG 2 cut(s) 18, 337
MspI CCGG 2 cut(s) 521, 787
MvnI CGCG 2 cut(s) 200, 361
MwoI GCNNNNNNNGC 6 cut(s) 15, 114, 164, 358, 403, 769
NcoI CCATGG 1 cut(s) 664
NdeII GATC 6 cut(s) 92, 169, 276, 282, 716, 767
NlaIII CATG 4 cut(s) 356, 496, 668, 716
NmeAIII GCCGAG 1 cut(s) 111
NmuCI GTSAC 2 cut(s) 680, 782
NspI RCATGY 2 cut(s) 356, 496
PaeI GCATGC 1 cut(s) 356
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PdmI GAANNNNTTC 1 cut(s) 42
PfeI GAWTC 1 cut(s) 24
PkrI GCNGC 6 cut(s) 20, 107, 186, 424, 549, 751
Ple19I CGATCG 2 cut(s) 279, 770
PleI GAGTC 3 cut(s) 78, 141, 242
PpsI GAGTC 3 cut(s) 78, 141, 242
Psp1406I AACGTT 1 cut(s) 63
PspEI GGTNACC 1 cut(s) 782
PspPI GGNCC 1 cut(s) 504
PvuI CGATCG 2 cut(s) 279, 770
PvuII CAGCTG 2 cut(s) 18, 337
RsaI GTAC 2 cut(s) 602, 760
RsaNI GTAC 2 cut(s) 601, 759
RseI CAYNNNNRTG 1 cut(s) 542
SaqAI TTAA 2 cut(s) 5, 473
SatI GCNGC 6 cut(s) 19, 106, 185, 423, 548, 750
Sau3AI GATC 6 cut(s) 92, 169, 276, 282, 716, 767
Sau96I GGNCC 1 cut(s) 504
SchI GAGTC 3 cut(s) 79, 142, 242
SduI GDGCHC 2 cut(s) 399, 587
SetI ASST 7 cut(s) 20, 45, 66, 339, 427, 590, 617
SfaNI GCATC 1 cut(s) 656
SmiMI CAYNNNNRTG 1 cut(s) 542
SphI GCATGC 1 cut(s) 356
Sse9I AATT 3 cut(s) 364, 576, 628
SsiI CCGC 6 cut(s) 21, 184, 212, 232, 565, 749
StyI CCWWGG 1 cut(s) 664
TaaI ACNGT 3 cut(s) 391, 758, 782
TaiI ACGT 4 cut(s) 45, 66, 590, 617
TaqI TCGA 5 cut(s) 68, 131, 144, 174, 251
TasI AATT 3 cut(s) 364, 576, 628
TauI GCSGC 2 cut(s) 187, 752
TfiI GAWTC 1 cut(s) 24
Tru1I TTAA 2 cut(s) 5, 473
Tru9I TTAA 2 cut(s) 5, 473
TscAI CASTG 1 cut(s) 561
TseFI GTSAC 2 cut(s) 680, 782
TseI GCWGC 4 cut(s) 18, 105, 422, 547
Tsp45I GTSAC 2 cut(s) 680, 782
TspDTI ATGAA 3 cut(s) 66, 497, 526
TspRI CASTG 1 cut(s) 561
VneI GTGCAC 1 cut(s) 583
XceI RCATGY 2 cut(s) 356, 496
XcmI CCANNNNNNNNNTGG 1 cut(s) 334
XmnI GAANNNNTTC 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.