MD07G1211400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003633
Physical Location & Seq
Forward (+)
28995980 .. 28998194
2215 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1211400.v1.1.491

Sequence Viewer

Length: 741 bp
ATGTTGAGCCCCTTCAACTGTGCCACTTTTAATCACCAAGAAGAAGATGATGATGAGCCCATGATTTCAACTTCACCTCCAACTCCAAGAAAATCATCGAGATCGAAAAGCGCTGGCCTGATTTACAGAAGACACAACAAGGACAGCAAAAACCCTTATTCGGACAGCGGCCTCGACAAGTTTTATGCACTTGTAGCTGACCTAGAAGAGAAAAGGAAAAAGATTTACGCACAAATGGACCCGAAAGACATATCTTTCGTGCGGTTCGTGTTCAAGGACGAGACTGATACAAGTGCTCCGGTTCCAATCGTGATAAAAAGGAAAGACCAAAGGGACAAAACAAAGACAACTGGTAGTCCTGAAGAGAAGCGTTCAAACCGTAATTCAGAAACCTTGGAAAATATTGACCAAGCCCACATCGACAAGAAAGACGTGAAGCAGAAGCAACCGGGATCGATATCCGATCAAGAGAGCAAGAAGGGGAGTTTTTCAAGTTCGAACATGAAGTCGGATGAGTGGAGAAGGCCATCTTATTATTTGCCGGTCGTTGTGATCTTTATTTTGGTGTTGTTGACTTTGTCTGGGCGGTCTTTTGCAATACTGTGTACGTCTATATTCTGGTACGCGATTCCATCGTTGAAAGAGAGTTCGAGTAGTACAAGAAGGCCGGCGGCATCAAAGAAGAAAGATTATATTAGAAGATTGAGTGACAAGAAGATGGTAGTTACTGAGGGAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

247

Amino Acids

28.1

Weight (kDa)

9.49

Isoelectric Point (pI)

71.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 626
AciI CCGC 4 cut(s) 168, 262, 586, 671
AclWI GGATC 1 cut(s) 460
AcsI RAATTY 1 cut(s) 735
AcuI CTGAAG 1 cut(s) 381
AfaI GTAC 3 cut(s) 607, 623, 658
AfeI AGCGCT 1 cut(s) 112
AfiI CCNNNNNNNGG 1 cut(s) 160
AgsI TTSAA 6 cut(s) 16, 69, 274, 375, 492, 640
AjiI CACGTC 1 cut(s) 433
AluBI AGCT 1 cut(s) 197
AluI AGCT 1 cut(s) 197
Alw21I GWGCWC 1 cut(s) 298
Alw26I GTCTC 1 cut(s) 275
AlwI GGATC 1 cut(s) 460
Aor51HI AGCGCT 1 cut(s) 112
AoxI GGCC 4 cut(s) 115, 169, 524, 665
ApoI RAATTY 1 cut(s) 735
ArsI GACNNNNNNTTYG 4 cut(s) 239, 271, 491, 523
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 1 cut(s) 238
AsuC2I CCSGG 1 cut(s) 450
AsuHPI GGTGA 2 cut(s) 26, 66
AsuII TTCGAA 1 cut(s) 497
AvaII GGWCC 1 cut(s) 238
BanII GRGCYC 2 cut(s) 11, 60
BbsI GAAGAC 1 cut(s) 136
Bbv12I GWGCWC 1 cut(s) 298
BccI CCATC 3 cut(s) 535, 640, 712
BcnI CCSGG 1 cut(s) 450
BcoDI GTCTC 1 cut(s) 275
BfaI CTAG 1 cut(s) 203
BfoI RGCGCY 1 cut(s) 114
BisI GCNGC 2 cut(s) 169, 672
BlsI GCNGC 2 cut(s) 170, 673
Bme1390I CCNGG 1 cut(s) 450
Bme18I GGWCC 1 cut(s) 238
BmgBI CACGTC 1 cut(s) 433
BmgT120I GGNCC 1 cut(s) 238
BmiI GGNNCC 2 cut(s) 240, 303
BmrFI CCNGG 1 cut(s) 450
BmsI GCATC 1 cut(s) 683
BpiI GAAGAC 1 cut(s) 136
Bpu14I TTCGAA 1 cut(s) 497
BpuMI CCSGG 1 cut(s) 450
Bsa29I ATCGAT 1 cut(s) 455
BsaBI GATNNNNATC 1 cut(s) 457
BsaJI CCNNGG 1 cut(s) 393
BsaWI WCCGGW 1 cut(s) 298
BsaXI ACNNNNNCTCC 4 cut(s) 61, 91, 280, 310
Bsc4I CCNNNNNNNGG 1 cut(s) 160
Bse118I RCCGGY 2 cut(s) 541, 667
Bse1I ACTGG 1 cut(s) 355
Bse8I GATNNNNATC 1 cut(s) 457
BseCI ATCGAT 1 cut(s) 455
BseDI CCNNGG 1 cut(s) 393
BseGI GGATG 1 cut(s) 517
BseJI GATNNNNATC 1 cut(s) 457
BseLI CCNNNNNNNGG 1 cut(s) 160
BseMII CTCAG 1 cut(s) 720
BseNI ACTGG 1 cut(s) 355
Bsh1236I CGCG 1 cut(s) 626
Bsh1285I CGRYCG 1 cut(s) 546
BshFI GGCC 4 cut(s) 117, 171, 526, 667
BshVI ATCGAT 1 cut(s) 455
BsiEI CGRYCG 1 cut(s) 546
BsiHKAI GWGCWC 1 cut(s) 298
BsiSI CCGG 4 cut(s) 299, 449, 542, 668
BslFI GGGAC 1 cut(s) 347
BslI CCNNNNNNNGG 1 cut(s) 160
BsmAI GTCTC 1 cut(s) 275
BsmFI GGGAC 1 cut(s) 347
BsnI GGCC 4 cut(s) 117, 171, 526, 667
Bsp119I TTCGAA 1 cut(s) 497
Bsp1286I GDGCHC 3 cut(s) 11, 60, 298
Bsp143I GATC 4 cut(s) 101, 452, 463, 552
BspACI CCGC 4 cut(s) 168, 262, 586, 671
BspANI GGCC 4 cut(s) 117, 171, 526, 667
BspCNI CTCAG 1 cut(s) 721
BspDI ATCGAT 1 cut(s) 455
BspFNI CGCG 1 cut(s) 626
BspLI GGNNCC 2 cut(s) 240, 303
BspPI GGATC 1 cut(s) 460
BspT104I TTCGAA 1 cut(s) 497
BsrFI RCCGGY 2 cut(s) 541, 667
BsrI ACTGG 1 cut(s) 355
BssAI RCCGGY 2 cut(s) 541, 667
BssECI CCNNGG 1 cut(s) 393
BssMI GATC 4 cut(s) 101, 452, 463, 552
BssT1I CCWWGG 1 cut(s) 393
Bst4CI ACNGT 3 cut(s) 20, 380, 603
Bst6I CTCTTC 2 cut(s) 201, 357
BstBI TTCGAA 1 cut(s) 497
BstC8I GCNNGC 2 cut(s) 115, 669
BstDEI CTNAG 1 cut(s) 729
BstF5I GGATG 1 cut(s) 517
BstFNI CGCG 1 cut(s) 626
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 1 cut(s) 113
BstKTI GATC 4 cut(s) 104, 455, 466, 555
BstMAI GTCTC 1 cut(s) 275
BstMBI GATC 4 cut(s) 101, 452, 463, 552
BstMCI CGRYCG 1 cut(s) 546
BstMWI GCNNNNNNNGC 1 cut(s) 194
BstSCI CCNGG 1 cut(s) 448
BstUI CGCG 1 cut(s) 626
BstV2I GAAGAC 1 cut(s) 136
Bsu15I ATCGAT 1 cut(s) 455
BsuRI GGCC 4 cut(s) 117, 171, 526, 667
BsuTUI ATCGAT 1 cut(s) 455
BtrI CACGTC 1 cut(s) 433
BtsCI GGATG 1 cut(s) 517
Cac8I GCNNGC 2 cut(s) 115, 669
CfoI GCGC 1 cut(s) 113
Cfr10I RCCGGY 2 cut(s) 541, 667
Cfr13I GGNCC 1 cut(s) 238
ClaI ATCGAT 1 cut(s) 455
Csp6I GTAC 3 cut(s) 606, 622, 657
CviAII CATG 2 cut(s) 61, 502
CviJI RGCY 8 cut(s) 9, 58, 117, 171, 197, 413, 526, 667
CviKI_1 RGCY 8 cut(s) 9, 58, 117, 171, 197, 413, 526, 667
CviQI GTAC 3 cut(s) 606, 622, 657
DdeI CTNAG 1 cut(s) 729
DpnI GATC 4 cut(s) 103, 454, 465, 554
DpnII GATC 4 cut(s) 101, 452, 463, 552
Eam1104I CTCTTC 2 cut(s) 201, 357
EarI CTCTTC 2 cut(s) 201, 357
Eco130I CCWWGG 1 cut(s) 393
Eco24I GRGCYC 2 cut(s) 11, 60
Eco32I GATATC 1 cut(s) 459
Eco47I GGWCC 1 cut(s) 238
Eco47III AGCGCT 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 381
EcoRV GATATC 1 cut(s) 459
EcoT14I CCWWGG 1 cut(s) 393
EcoT38I GRGCYC 2 cut(s) 11, 60
ErhI CCWWGG 1 cut(s) 393
FaeI CATG 2 cut(s) 64, 505
FaiI YATR 6 cut(s) 62, 186, 251, 503, 614, 693
FalI AAGNNNNNCTT 2 cut(s) 514, 546
FaqI GGGAC 1 cut(s) 347
FatI CATG 2 cut(s) 60, 501
Fnu4HI GCNGC 2 cut(s) 169, 672
FokI GGATG 1 cut(s) 524
FriOI GRGCYC 2 cut(s) 11, 60
Fsp4HI GCNGC 2 cut(s) 169, 672
FspBI CTAG 1 cut(s) 203
GlaI GCGC 1 cut(s) 112
GluI GCNGC 2 cut(s) 169, 672
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 4 cut(s) 117, 171, 526, 667
HapII CCGG 4 cut(s) 299, 449, 542, 668
HhaI GCGC 1 cut(s) 113
Hin1II CATG 2 cut(s) 64, 505
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HincII GTYRAC 1 cut(s) 573
HindII GTYRAC 1 cut(s) 573
HinfI GANTC 1 cut(s) 628
HpaII CCGG 4 cut(s) 299, 449, 542, 668
HphI GGTGA 2 cut(s) 26, 66
Hpy166II GTNNAC 2 cut(s) 573, 606
Hpy188I TCNGA 4 cut(s) 163, 388, 463, 511
Hpy188III TCNNGA 4 cut(s) 99, 310, 359, 467
Hpy8I GTNNAC 2 cut(s) 573, 606
HpyAV CCTTC 4 cut(s) 22, 472, 516, 657
HpyCH4III ACNGT 3 cut(s) 20, 380, 603
HpyCH4IV ACGT 2 cut(s) 432, 608
HpyCH4V TGCA 2 cut(s) 188, 596
HpyF10VI GCNNNNNNNGC 1 cut(s) 194
HpyF3I CTNAG 1 cut(s) 729
HpySE526I ACGT 2 cut(s) 432, 608
Hsp92II CATG 2 cut(s) 64, 505
HspAI GCGC 1 cut(s) 111
KroI GCCGGC 1 cut(s) 667
KroNI GCCGGC 1 cut(s) 669
Kzo9I GATC 4 cut(s) 101, 452, 463, 552
LmnI GCTCC 1 cut(s) 301
LweI GCATC 1 cut(s) 683
MaeI CTAG 1 cut(s) 203
MaeII ACGT 2 cut(s) 432, 608
MaeIII GTNAC 2 cut(s) 707, 724
MalI GATC 4 cut(s) 103, 454, 465, 554
MboI GATC 4 cut(s) 101, 452, 463, 552
MboII GAAGA 8 cut(s) 53, 56, 141, 218, 374, 694, 711, 727
MhlI GDGCHC 3 cut(s) 11, 60, 298
MluCI AATT 2 cut(s) 382, 735
MmeI TCCRAC 2 cut(s) 104, 489
MnlI CCTC 3 cut(s) 87, 182, 724
MroNI GCCGGC 1 cut(s) 667
MseI TTAA 1 cut(s) 30
MspA1I CMGCKG 1 cut(s) 168
MspI CCGG 4 cut(s) 299, 449, 542, 668
MspR9I CCNGG 1 cut(s) 450
MvnI CGCG 1 cut(s) 626
MwoI GCNNNNNNNGC 1 cut(s) 194
NaeI GCCGGC 1 cut(s) 669
NciI CCSGG 1 cut(s) 450
NdeII GATC 4 cut(s) 101, 452, 463, 552
NgoMIV GCCGGC 1 cut(s) 667
NlaIII CATG 2 cut(s) 64, 505
NlaIV GGNNCC 2 cut(s) 240, 303
NmuCI GTSAC 1 cut(s) 707
NspV TTCGAA 1 cut(s) 497
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PdiI GCCGGC 1 cut(s) 669
PfeI GAWTC 1 cut(s) 628
PflFI GACNNNGTC 1 cut(s) 577
PkrI GCNGC 2 cut(s) 170, 673
PspN4I GGNNCC 2 cut(s) 240, 303
PspPI GGNCC 1 cut(s) 238
PsyI GACNNNGTC 1 cut(s) 577
RsaI GTAC 3 cut(s) 607, 623, 658
RsaNI GTAC 3 cut(s) 606, 622, 657
SaqAI TTAA 1 cut(s) 30
SatI GCNGC 2 cut(s) 169, 672
Sau3AI GATC 4 cut(s) 101, 452, 463, 552
Sau96I GGNCC 1 cut(s) 238
ScrFI CCNGG 1 cut(s) 450
SduI GDGCHC 3 cut(s) 11, 60, 298
SetI ASST 6 cut(s) 79, 199, 204, 395, 435, 611
SfaNI GCATC 1 cut(s) 683
SfuI TTCGAA 1 cut(s) 497
SinI GGWCC 1 cut(s) 238
Sse9I AATT 2 cut(s) 382, 735
SsiI CCGC 4 cut(s) 168, 262, 586, 671
SspI AATATT 1 cut(s) 403
SspMI CTAG 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 448
StyI CCWWGG 1 cut(s) 393
TaaI ACNGT 3 cut(s) 20, 380, 603
TaiI ACGT 2 cut(s) 435, 611
TaqI TCGA 7 cut(s) 98, 104, 174, 420, 455, 497, 650
TasI AATT 2 cut(s) 382, 735
TatI WGTACW 1 cut(s) 656
TauI GCSGC 2 cut(s) 171, 674
TfiI GAWTC 1 cut(s) 628
Tru1I TTAA 1 cut(s) 30
Tru9I TTAA 1 cut(s) 30
TseFI GTSAC 1 cut(s) 707
Tsp45I GTSAC 1 cut(s) 707
TspDTI ATGAA 1 cut(s) 518
Tth111I GACNNNGTC 1 cut(s) 577
VpaK11BI GGWCC 1 cut(s) 238
XapI RAATTY 1 cut(s) 735
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.