FvH4_2g33050

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003633
Physical Location & Seq
Forward (+)
24888155 .. 24889933
1779 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33050.t1

Sequence Viewer

Length: 795 bp
ATGTTGAGCCCTTTCAGCTGTGGCTTTTTCCACCCAGAAACATCTGATGAGTACGACGAACCCATCATCACAAACAGTCCTTGCTCGACTCCAAGAAGATCATCAGGCAGAAGAAGTGGTGGTCTCATTCATAGAATCCATCACAGCAACAAGGACAAGAACCCTTATTCGACTCGAGGCCTCGACAAGTTCTCTGCACTTGTGGCTGATCTTGAAGAGAAACGCAAGCAGATTTATGCTCAAACCGGCTCTGAGGACGATACCTTTGTTCGGTTTGTGTACAAGGACTCAAACGACAACGTTCCTGTTCCGATTGTGCTCAAATTAAAGGAGAAGAAGCAAGACGAGCAGAAGAAGAAGCCTCACCATCATGATAATCATGAAGAAAAGCATGCAGAAGGGTTAGATAAAATGACCGTTAAGCCAGCGCCCGCCAAAGAAGAAGCAGTACTTGAGCAACAACCTAAGAAGAGCAACATGTTTGACTTCTGGTCAGACATCAACTTCAGACGACCGTCGTTTTATCTTCCGGTGGTTGTAGTGCTGATTTTGGTGTTGCTCGCCGTGTTTGGGCGGTCGGTGGCGATTTTGTGTACCTCTATAGGTTGGTATGCGATTCCAACACTGAAAGAACAGTCTGCTGCAAAGAGAAGAAAATCTTCGAAGCCAAAACAACATGTGAAAAGGTCGAGTGAGCATAAGATGGTGATCAGTACTAGTGAATTATCTTCTCCGAAAAGTGACAGCAATTACACTCCCCGATCCCGGAAACATGATCACCGGAAAAGTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

265

Amino Acids

30.31

Weight (kDa)

9.56

Isoelectric Point (pI)

71.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 432, 574
AclI AACGTT 1 cut(s) 300
AclWI GGATC 1 cut(s) 756
AcuI CTGAAG 1 cut(s) 490
AfaI GTAC 5 cut(s) 53, 281, 450, 595, 715
AfiI CCNNNNNNNGG 3 cut(s) 270, 570, 765
AflIII ACRYGT 2 cut(s) 477, 676
AgsI TTSAA 1 cut(s) 215
AhdI GACNNNNNGTC 1 cut(s) 490
AhlI ACTAGT 1 cut(s) 716
AluBI AGCT 1 cut(s) 18
AluI AGCT 1 cut(s) 18
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 1 cut(s) 756
Ama87I CYCGRG 1 cut(s) 174
AoxI GGCC 1 cut(s) 178
ApeKI GCWGC 1 cut(s) 641
ArsI GACNNNNNNTTYG 2 cut(s) 248, 280
Asp700I GAANNNNTTC 1 cut(s) 658
AspLEI GCGC 1 cut(s) 430
AsuC2I CCSGG 1 cut(s) 766
AsuHPI GGTGA 3 cut(s) 356, 718, 770
AsuII TTCGAA 1 cut(s) 662
AvaI CYCGRG 1 cut(s) 174
BaeI ACNNNNGTAYC 2 cut(s) 252, 285
BanII GRGCYC 1 cut(s) 11
BarI GAAGNNNNNNTAC 2 cut(s) 432, 464
Bbv12I GWGCWC 1 cut(s) 321
BbvI GCAGC 1 cut(s) 628
BccI CCATC 4 cut(s) 71, 147, 375, 697
BceAI ACGGC 1 cut(s) 548
BclI TGATCA 2 cut(s) 708, 775
BcnI CCSGG 1 cut(s) 766
BcoDI GTCTC 1 cut(s) 128
BcuI ACTAGT 1 cut(s) 716
BfaI CTAG 1 cut(s) 717
BfmI CTRYAG 1 cut(s) 600
BfoI RGCGCY 1 cut(s) 431
BisI GCNGC 1 cut(s) 642
BlsI GCNGC 1 cut(s) 643
BmcAI AGTACT 2 cut(s) 450, 715
Bme1390I CCNGG 1 cut(s) 766
BmeRI GACNNNNNGTC 1 cut(s) 490
BmeT110I CYCGRG 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 766
BoxI GACNNNNGTC 1 cut(s) 514
Bpu14I TTCGAA 1 cut(s) 662
BpuEI CTTGAG 1 cut(s) 473
BpuMI CCSGG 1 cut(s) 766
BsaBI GATNNNNATC 1 cut(s) 707
BsaI GGTCTC 1 cut(s) 128
BsaWI WCCGGW 2 cut(s) 529, 780
Bsc4I CCNNNNNNNGG 3 cut(s) 270, 570, 765
Bse118I RCCGGY 1 cut(s) 245
Bse8I GATNNNNATC 1 cut(s) 707
BseJI GATNNNNATC 1 cut(s) 707
BseLI CCNNNNNNNGG 3 cut(s) 270, 570, 765
BseMII CTCAG 1 cut(s) 243
BseXI GCAGC 1 cut(s) 628
BsgI GTGCAG 1 cut(s) 180
Bsh1285I CGRYCG 2 cut(s) 515, 578
BshFI GGCC 1 cut(s) 180
BsiEI CGRYCG 2 cut(s) 515, 578
BsiHKAI GWGCWC 1 cut(s) 321
BsiHKCI CYCGRG 1 cut(s) 174
BsiSI CCGG 4 cut(s) 246, 530, 766, 781
BslI CCNNNNNNNGG 3 cut(s) 270, 570, 765
BsmAI GTCTC 1 cut(s) 128
BsnI GGCC 1 cut(s) 180
Bso31I GGTCTC 1 cut(s) 128
BsoBI CYCGRG 1 cut(s) 174
Bsp119I TTCGAA 1 cut(s) 662
Bsp1286I GDGCHC 2 cut(s) 11, 321
Bsp1407I TGTACA 1 cut(s) 279
Bsp143I GATC 5 cut(s) 98, 208, 708, 761, 775
BspACI CCGC 2 cut(s) 432, 574
BspANI GGCC 1 cut(s) 180
BspCNI CTCAG 1 cut(s) 244
BspHI TCATGA 2 cut(s) 370, 379
BspPI GGATC 1 cut(s) 756
BspQI GCTCTTC 1 cut(s) 464
BspT104I TTCGAA 1 cut(s) 662
BspTNI GGTCTC 1 cut(s) 128
BsrFI RCCGGY 1 cut(s) 245
BsrGI TGTACA 1 cut(s) 279
BssAI RCCGGY 1 cut(s) 245
BssMI GATC 5 cut(s) 98, 208, 708, 761, 775
Bst4CI ACNGT 4 cut(s) 77, 418, 516, 636
Bst6I CTCTTC 2 cut(s) 210, 464
BstAUI TGTACA 1 cut(s) 279
BstBI TTCGAA 1 cut(s) 662
BstC8I GCNNGC 5 cut(s) 227, 393, 426, 432, 561
BstDEI CTNAG 2 cut(s) 252, 465
BstH2I RGCGCY 1 cut(s) 431
BstHHI GCGC 1 cut(s) 430
BstKTI GATC 5 cut(s) 101, 211, 711, 764, 778
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 5 cut(s) 98, 208, 708, 761, 775
BstMCI CGRYCG 2 cut(s) 515, 578
BstMWI GCNNNNNNNGC 3 cut(s) 15, 203, 346
BstNSI RCATGY 3 cut(s) 395, 481, 680
BstPAI GACNNNNGTC 1 cut(s) 514
BstSCI CCNGG 1 cut(s) 764
BstSFI CTRYAG 1 cut(s) 600
BstV1I GCAGC 1 cut(s) 628
BsuRI GGCC 1 cut(s) 180
BtsIMutI CAGTG 1 cut(s) 623
Cac8I GCNNGC 5 cut(s) 227, 393, 426, 432, 561
CciI TCATGA 2 cut(s) 370, 379
CfoI GCGC 1 cut(s) 430
Cfr10I RCCGGY 1 cut(s) 245
Csp6I GTAC 5 cut(s) 52, 280, 449, 594, 714
CviAII CATG 6 cut(s) 371, 380, 392, 478, 677, 773
CviJI RGCY 9 cut(s) 9, 18, 24, 180, 206, 249, 361, 424, 667
CviKI_1 RGCY 9 cut(s) 9, 18, 24, 180, 206, 249, 361, 424, 667
CviQI GTAC 5 cut(s) 52, 280, 449, 594, 714
DdeI CTNAG 2 cut(s) 252, 465
DpnI GATC 5 cut(s) 100, 210, 710, 763, 777
DpnII GATC 5 cut(s) 98, 208, 708, 761, 775
DriI GACNNNNNGTC 1 cut(s) 490
Eam1104I CTCTTC 2 cut(s) 210, 464
Eam1105I GACNNNNNGTC 1 cut(s) 490
EarI CTCTTC 2 cut(s) 210, 464
Eco147I AGGCCT 1 cut(s) 180
Eco24I GRGCYC 1 cut(s) 11
Eco31I GGTCTC 1 cut(s) 128
Eco57I CTGAAG 1 cut(s) 490
Eco88I CYCGRG 1 cut(s) 174
EcoT38I GRGCYC 1 cut(s) 11
FaeI CATG 6 cut(s) 374, 383, 395, 481, 680, 776
FalI AAGNNNNNCTT 4 cut(s) 435, 467, 643, 675
FatI CATG 6 cut(s) 370, 379, 391, 477, 676, 772
FauI CCCGC 1 cut(s) 439
FbaI TGATCA 2 cut(s) 708, 775
Fnu4HI GCNGC 1 cut(s) 642
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 1 cut(s) 642
FspBI CTAG 1 cut(s) 717
GlaI GCGC 1 cut(s) 429
GluI GCNGC 1 cut(s) 642
HaeII RGCGCY 1 cut(s) 431
HaeIII GGCC 1 cut(s) 180
HapII CCGG 4 cut(s) 246, 530, 766, 781
HhaI GCGC 1 cut(s) 430
Hin1II CATG 6 cut(s) 374, 383, 395, 481, 680, 776
Hin6I GCGC 1 cut(s) 428
HinP1I GCGC 1 cut(s) 428
HinfI GANTC 5 cut(s) 88, 135, 172, 287, 616
HpaII CCGG 4 cut(s) 246, 530, 766, 781
HphI GGTGA 3 cut(s) 356, 718, 770
Hpy166II GTNNAC 2 cut(s) 280, 594
Hpy188I TCNGA 6 cut(s) 46, 253, 312, 496, 509, 735
Hpy188III TCNNGA 3 cut(s) 212, 371, 380
Hpy8I GTNNAC 2 cut(s) 280, 594
Hpy99I CGWCG 2 cut(s) 59, 520
HpyAV CCTTC 1 cut(s) 392
HpyCH4III ACNGT 4 cut(s) 77, 418, 516, 636
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 3 cut(s) 197, 395, 644
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 203, 346
HpyF3I CTNAG 2 cut(s) 252, 465
HpySE526I ACGT 1 cut(s) 300
Hsp92II CATG 6 cut(s) 374, 383, 395, 481, 680, 776
HspAI GCGC 1 cut(s) 428
Ksp22I TGATCA 2 cut(s) 708, 775
Kzo9I GATC 5 cut(s) 98, 208, 708, 761, 775
LguI GCTCTTC 1 cut(s) 464
LpnPI CCDG 8 cut(s) 48, 90, 259, 318, 438, 475, 543, 779
Lsp1109I GCAGC 1 cut(s) 628
MaeI CTAG 1 cut(s) 717
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 1 cut(s) 740
MalI GATC 5 cut(s) 100, 210, 710, 763, 777
MboI GATC 5 cut(s) 98, 208, 708, 761, 775
MhlI GDGCHC 2 cut(s) 11, 321
MluCI AATT 3 cut(s) 323, 722, 748
MlyI GAGTC 3 cut(s) 82, 166, 281
MmeI TCCRAC 1 cut(s) 644
MnlI CCTC 5 cut(s) 170, 191, 247, 372, 607
MroXI GAANNNNTTC 1 cut(s) 658
MseI TTAA 2 cut(s) 326, 420
MslI CAYNNNNRTG 1 cut(s) 369
MspA1I CMGCKG 1 cut(s) 18
MspI CCGG 4 cut(s) 246, 530, 766, 781
MspR9I CCNGG 1 cut(s) 766
MwoI GCNNNNNNNGC 3 cut(s) 15, 203, 346
NciI CCSGG 1 cut(s) 766
NdeII GATC 5 cut(s) 98, 208, 708, 761, 775
NlaIII CATG 6 cut(s) 374, 383, 395, 481, 680, 776
NmuCI GTSAC 1 cut(s) 740
NspI RCATGY 3 cut(s) 395, 481, 680
NspV TTCGAA 1 cut(s) 662
PaeI GCATGC 1 cut(s) 395
PaeR7I CTCGAG 1 cut(s) 174
PagI TCATGA 2 cut(s) 370, 379
PceI AGGCCT 1 cut(s) 180
PciI ACATGT 2 cut(s) 477, 676
PciSI GCTCTTC 1 cut(s) 464
PdmI GAANNNNTTC 1 cut(s) 658
PfeI GAWTC 2 cut(s) 135, 616
PfoI TCCNGGA 1 cut(s) 764
PkrI GCNGC 1 cut(s) 643
PleI GAGTC 3 cut(s) 82, 166, 281
PpsI GAGTC 3 cut(s) 82, 166, 281
PscI ACATGT 2 cut(s) 477, 676
PshAI GACNNNNGTC 1 cut(s) 514
Psp1406I AACGTT 1 cut(s) 300
PspXI VCTCGAGB 1 cut(s) 174
PvuII CAGCTG 1 cut(s) 18
RsaI GTAC 5 cut(s) 53, 281, 450, 595, 715
RsaNI GTAC 5 cut(s) 52, 280, 449, 594, 714
RseI CAYNNNNRTG 1 cut(s) 369
SapI GCTCTTC 1 cut(s) 464
SaqAI TTAA 2 cut(s) 326, 420
SatI GCNGC 1 cut(s) 642
Sau3AI GATC 5 cut(s) 98, 208, 708, 761, 775
ScaI AGTACT 2 cut(s) 450, 715
SchI GAGTC 3 cut(s) 82, 166, 281
ScrFI CCNGG 1 cut(s) 766
SduI GDGCHC 2 cut(s) 11, 321
SetI ASST 7 cut(s) 20, 266, 303, 466, 599, 607, 689
SfcI CTRYAG 1 cut(s) 600
Sfr274I CTCGAG 1 cut(s) 174
SfuI TTCGAA 1 cut(s) 662
SlaI CTCGAG 1 cut(s) 174
SmiMI CAYNNNNRTG 1 cut(s) 369
SmlI CTYRAG 2 cut(s) 174, 452
SmoI CTYRAG 2 cut(s) 174, 452
SpeI ACTAGT 1 cut(s) 716
SphI GCATGC 1 cut(s) 395
Sse9I AATT 3 cut(s) 323, 722, 748
SseBI AGGCCT 1 cut(s) 180
SsiI CCGC 2 cut(s) 432, 574
SspMI CTAG 1 cut(s) 717
StuI AGGCCT 1 cut(s) 180
StyD4I CCNGG 1 cut(s) 764
TaaI ACNGT 4 cut(s) 77, 418, 516, 636
TaiI ACGT 1 cut(s) 303
TaqI TCGA 6 cut(s) 86, 170, 175, 183, 662, 689
TasI AATT 3 cut(s) 323, 722, 748
TatI WGTACW 3 cut(s) 279, 448, 713
TfiI GAWTC 2 cut(s) 135, 616
Tru1I TTAA 2 cut(s) 326, 420
Tru9I TTAA 2 cut(s) 326, 420
TscAI CASTG 1 cut(s) 630
TseFI GTSAC 1 cut(s) 740
TseI GCWGC 1 cut(s) 641
Tsp45I GTSAC 1 cut(s) 740
TspDTI ATGAA 2 cut(s) 119, 396
TspRI CASTG 1 cut(s) 630
XceI RCATGY 3 cut(s) 395, 481, 680
XhoI CTCGAG 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 658
XspI CTAG 1 cut(s) 717
ZrmI AGTACT 2 cut(s) 450, 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.