AT1G23145

Cell signaling peptide that may regulate plant stress, growth, and development. Mediates a rapid alkalinization of extracellular space by mediating a transient increase in the cytoplasmic Ca(2 ) concentration leading to a calcium-dependent signaling events through a cell surface receptor and a concomitant activation of some intracellular mitogen-activated protein kinases (By similarity)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006496
Physical Location & Seq
Forward (+)
8203933 .. 8204318
386 bp
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UTR
Exon/CDS
Intron
AT1G23145.1

Sequence Viewer

Length: 294 bp
ATGGAAGCAAGACATATGCTCGTGACAATACTCTTGTTATCATTTGTGTTTATGAATATCATGAAGGTGGAAGCTCAAAAAGTAATTGGATATCCAGCAATAGGACGTGATGGTGCACGTGGATGTAGTCCTAAAGATCCTTCATGTCCACAACAACCGGAGAAGCCGTACAAGCGAGGATGTGAGAAAATTACAAGATGTGAACGTGATCGCCGTAAGCAAGCCCATCTTCGGAATCCTCGAAAAGTACTAGATGTAGTCGCAGTGATGGCAAAGGCCAAGCAGCTCTACTGA

Protein Analysis

97

Amino Acids

11.11

Weight (kDa)

9.97

Isoelectric Point (pI)

48.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 23 - 69 9.2e-08 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 131
AcvI CACGTG 1 cut(s) 119
AfaI GTAC 2 cut(s) 170, 249
AfiI CCNNNNNNNGG 2 cut(s) 101, 231
AjiI CACGTC 1 cut(s) 107
AluBI AGCT 2 cut(s) 74, 286
AluI AGCT 2 cut(s) 74, 286
Alw21I GWGCWC 1 cut(s) 118
Alw44I GTGCAC 1 cut(s) 114
AlwI GGATC 1 cut(s) 131
AoxI GGCC 1 cut(s) 276
ApaLI GTGCAC 1 cut(s) 114
ApeKI GCWGC 1 cut(s) 283
BaeGI GKGCMC 1 cut(s) 118
BauI CACGAG 1 cut(s) 20
BbrPI CACGTG 1 cut(s) 119
Bbv12I GWGCWC 1 cut(s) 118
BccI CCATC 3 cut(s) 104, 234, 262
BceAI ACGGC 2 cut(s) 151, 198
BfaI CTAG 1 cut(s) 251
BisI GCNGC 1 cut(s) 284
BlsI GCNGC 1 cut(s) 285
BmcAI AGTACT 1 cut(s) 249
BmgBI CACGTC 1 cut(s) 107
BsaAI YACGTR 1 cut(s) 119
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 231
BseGI GGATG 2 cut(s) 128, 185
BseLI CCNNNNNNNGG 2 cut(s) 101, 231
BseSI GKGCMC 1 cut(s) 118
BshFI GGCC 1 cut(s) 278
BsiHKAI GWGCWC 1 cut(s) 118
BsiSI CCGG 1 cut(s) 158
BslI CCNNNNNNNGG 2 cut(s) 101, 231
BsnI GGCC 1 cut(s) 278
Bsp1286I GDGCHC 1 cut(s) 118
Bsp143I GATC 2 cut(s) 136, 208
BspANI GGCC 1 cut(s) 278
BspHI TCATGA 1 cut(s) 60
BspPI GGATC 1 cut(s) 131
BssMI GATC 2 cut(s) 136, 208
BssSI CACGAG 1 cut(s) 20
Bst2BI CACGAG 1 cut(s) 20
BstBAI YACGTR 1 cut(s) 119
BstC8I GCNNGC 1 cut(s) 222
BstF5I GGATG 2 cut(s) 128, 185
BstKTI GATC 2 cut(s) 139, 211
BstMBI GATC 2 cut(s) 136, 208
BstMWI GCNNNNNNNGC 2 cut(s) 172, 269
BstSLI GKGCMC 1 cut(s) 118
BstX2I RGATCY 1 cut(s) 136
BstYI RGATCY 1 cut(s) 136
BsuRI GGCC 1 cut(s) 278
BtrI CACGTC 1 cut(s) 107
BtsCI GGATG 2 cut(s) 128, 185
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 1 cut(s) 270
Cac8I GCNNGC 1 cut(s) 222
CciI TCATGA 1 cut(s) 60
Csp6I GTAC 2 cut(s) 169, 248
CviAII CATG 2 cut(s) 61, 144
CviJI RGCY 5 cut(s) 74, 166, 224, 278, 286
CviKI_1 RGCY 5 cut(s) 74, 166, 224, 278, 286
CviQI GTAC 2 cut(s) 169, 248
DpnI GATC 2 cut(s) 138, 210
DpnII GATC 2 cut(s) 136, 208
Eco32I GATATC 1 cut(s) 92
Eco72I CACGTG 1 cut(s) 119
EcoRV GATATC 1 cut(s) 92
FaeI CATG 2 cut(s) 64, 147
FaiI YATR 5 cut(s) 15, 17, 53, 62, 145
FalI AAGNNNNNCTT 2 cut(s) 213, 245
FatI CATG 2 cut(s) 60, 143
FauNDI CATATG 1 cut(s) 15
Fnu4HI GCNGC 1 cut(s) 284
FokI GGATG 2 cut(s) 135, 192
Fsp4HI GCNGC 1 cut(s) 284
FspBI CTAG 1 cut(s) 251
GluI GCNGC 1 cut(s) 284
HaeIII GGCC 1 cut(s) 278
HapII CCGG 1 cut(s) 158
Hin1II CATG 2 cut(s) 64, 147
HinfI GANTC 1 cut(s) 235
HpaII CCGG 1 cut(s) 158
Hpy166II GTNNAC 3 cut(s) 116, 149, 203
Hpy188I TCNGA 1 cut(s) 234
Hpy188III TCNNGA 2 cut(s) 22, 61
Hpy8I GTNNAC 3 cut(s) 116, 149, 203
HpyAV CCTTC 2 cut(s) 58, 150
HpyCH4IV ACGT 3 cut(s) 106, 118, 205
HpyCH4V TGCA 1 cut(s) 116
HpyF10VI GCNNNNNNNGC 2 cut(s) 172, 269
HpySE526I ACGT 3 cut(s) 106, 118, 205
Hsp92II CATG 2 cut(s) 64, 147
Kzo9I GATC 2 cut(s) 136, 208
LpnPI CCDG 2 cut(s) 108, 171
MaeI CTAG 1 cut(s) 251
MaeII ACGT 3 cut(s) 106, 118, 205
MaeIII GTNAC 1 cut(s) 22
MalI GATC 2 cut(s) 138, 210
MboI GATC 2 cut(s) 136, 208
MboII GAAGA 1 cut(s) 221
MflI RGATCY 1 cut(s) 136
MhlI GDGCHC 1 cut(s) 118
MluCI AATT 2 cut(s) 84, 189
MnlI CCTC 2 cut(s) 170, 249
MslI CAYNNNNRTG 2 cut(s) 65, 121
MspI CCGG 1 cut(s) 158
MwoI GCNNNNNNNGC 2 cut(s) 172, 269
NdeI CATATG 1 cut(s) 15
NdeII GATC 2 cut(s) 136, 208
NlaIII CATG 2 cut(s) 64, 147
NmuCI GTSAC 1 cut(s) 22
PagI TCATGA 1 cut(s) 60
PcsI WCGNNNNNNNCGW 2 cut(s) 211, 238
PfeI GAWTC 1 cut(s) 235
PkrI GCNGC 1 cut(s) 285
PmaCI CACGTG 1 cut(s) 119
PmlI CACGTG 1 cut(s) 119
Ppu21I YACGTR 1 cut(s) 119
PspCI CACGTG 1 cut(s) 119
PsuI RGATCY 1 cut(s) 136
RsaI GTAC 2 cut(s) 170, 249
RsaNI GTAC 2 cut(s) 169, 248
RseI CAYNNNNRTG 2 cut(s) 65, 121
SatI GCNGC 1 cut(s) 284
Sau3AI GATC 2 cut(s) 136, 208
ScaI AGTACT 1 cut(s) 249
SduI GDGCHC 1 cut(s) 118
SetI ASST 6 cut(s) 69, 76, 109, 121, 208, 288
SmiMI CAYNNNNRTG 2 cut(s) 65, 121
Sse9I AATT 2 cut(s) 84, 189
SspMI CTAG 1 cut(s) 251
TaiI ACGT 3 cut(s) 109, 121, 208
TaqI TCGA 1 cut(s) 241
TasI AATT 2 cut(s) 84, 189
TatI WGTACW 1 cut(s) 247
TfiI GAWTC 1 cut(s) 235
TscAI CASTG 1 cut(s) 270
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 1 cut(s) 283
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 3 cut(s) 68, 77, 132
TspRI CASTG 1 cut(s) 270
VneI GTGCAC 1 cut(s) 114
XspI CTAG 1 cut(s) 251
ZrmI AGTACT 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.