Rw2G043630

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006496
Physical Location & Seq
Reverse (-)
70086006 .. 70086275
270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G043630.1

Sequence Viewer

Length: 270 bp
ATGGCATCAGTTGTGTTCAAGGCAACAAGTGTTTTGTGCCTAACTATGTTTGTATCAGTTTTGCTGATTGAAGAAGTAGATGGCAATAAGTACATAGACTACGGAGCTATTAAAGGAGATGAAATTCCGGGATGCAGCGCTAAGCATCCAGAAAATTGTTACCCAAAAAGGGCAAATCGATATCAAAGAGGTTGTAACCCGGAAACGCGATGCAGACAAGTCCATACCACAACAGCACCCAAAGGCGAAAGTGTAGGAGCAACCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

89

Amino Acids

9.75

Weight (kDa)

8.65

Isoelectric Point (pI)

25.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 27 - 72 5.3e-11 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 208
AcsI RAATTY 1 cut(s) 123
AfaI GTAC 1 cut(s) 92
AfeI AGCGCT 1 cut(s) 139
AfiI CCNNNNNNNGG 1 cut(s) 169
AgsI TTSAA 2 cut(s) 19, 71
AluBI AGCT 1 cut(s) 107
AluI AGCT 1 cut(s) 107
Aor51HI AGCGCT 1 cut(s) 139
ApeKI GCWGC 1 cut(s) 135
ApoI RAATTY 1 cut(s) 123
AspLEI GCGC 1 cut(s) 140
AsuC2I CCSGG 2 cut(s) 129, 200
BbvI GCAGC 1 cut(s) 147
BccI CCATC 1 cut(s) 74
BcnI CCSGG 2 cut(s) 129, 200
BfoI RGCGCY 1 cut(s) 141
BisI GCNGC 1 cut(s) 136
BlpI GCTNAGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 137
Bme1390I CCNGG 2 cut(s) 129, 200
BmrFI CCNGG 2 cut(s) 129, 200
BmsI GCATC 4 cut(s) 14, 122, 154, 200
Bpu1102I GCTNAGC 1 cut(s) 141
BpuMI CCSGG 2 cut(s) 129, 200
Bsa29I ATCGAT 1 cut(s) 178
Bsc4I CCNNNNNNNGG 1 cut(s) 169
BseCI ATCGAT 1 cut(s) 178
BseGI GGATG 2 cut(s) 137, 145
BseLI CCNNNNNNNGG 1 cut(s) 169
BseXI GCAGC 1 cut(s) 147
Bsh1236I CGCG 1 cut(s) 208
BshVI ATCGAT 1 cut(s) 178
BsiSI CCGG 2 cut(s) 128, 200
BslI CCNNNNNNNGG 1 cut(s) 169
Bsp1720I GCTNAGC 1 cut(s) 141
BspDI ATCGAT 1 cut(s) 178
BspFNI CGCG 1 cut(s) 208
BstDEI CTNAG 1 cut(s) 141
BstF5I GGATG 2 cut(s) 137, 145
BstFNI CGCG 1 cut(s) 208
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 1 cut(s) 140
BstSCI CCNGG 2 cut(s) 127, 198
BstUI CGCG 1 cut(s) 208
BstV1I GCAGC 1 cut(s) 147
Bsu15I ATCGAT 1 cut(s) 178
BsuTUI ATCGAT 1 cut(s) 178
BtgZI GCGATG 1 cut(s) 223
BtsCI GGATG 2 cut(s) 137, 145
CfoI GCGC 1 cut(s) 140
ClaI ATCGAT 1 cut(s) 178
Csp6I GTAC 1 cut(s) 91
CviJI RGCY 1 cut(s) 107
CviKI_1 RGCY 1 cut(s) 107
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 1 cut(s) 141
Eco32I GATATC 1 cut(s) 182
Eco47III AGCGCT 1 cut(s) 139
EcoRV GATATC 1 cut(s) 182
FaiI YATR 3 cut(s) 47, 95, 225
Fnu4HI GCNGC 1 cut(s) 136
FokI GGATG 2 cut(s) 132, 144
Fsp4HI GCNGC 1 cut(s) 136
GlaI GCGC 1 cut(s) 139
GluI GCNGC 1 cut(s) 136
HaeII RGCGCY 1 cut(s) 141
HapII CCGG 2 cut(s) 128, 200
HhaI GCGC 1 cut(s) 140
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HpaII CCGG 2 cut(s) 128, 200
Hpy188III TCNNGA 1 cut(s) 149
HpyCH4V TGCA 2 cut(s) 135, 213
HpyF3I CTNAG 1 cut(s) 141
HspAI GCGC 1 cut(s) 138
LmnI GCTCC 2 cut(s) 104, 257
LpnPI CCDG 3 cut(s) 141, 162, 213
Lsp1109I GCAGC 1 cut(s) 147
LweI GCATC 4 cut(s) 14, 122, 154, 200
MaeIII GTNAC 2 cut(s) 158, 194
MboII GAAGA 1 cut(s) 83
MluCI AATT 2 cut(s) 123, 154
MnlI CCTC 1 cut(s) 182
MseI TTAA 1 cut(s) 111
MspI CCGG 2 cut(s) 128, 200
MspR9I CCNGG 2 cut(s) 129, 200
MvnI CGCG 1 cut(s) 208
NciI CCSGG 2 cut(s) 129, 200
PfoI TCCNGGA 1 cut(s) 127
PkrI GCNGC 1 cut(s) 137
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 1 cut(s) 136
ScrFI CCNGG 2 cut(s) 129, 200
SetI ASST 2 cut(s) 109, 193
SfaNI GCATC 4 cut(s) 14, 122, 154, 200
SgeI CNNG 9 cut(s) 31, 39, 140, 141, 161, 211, 212, 219, 230
Sse9I AATT 2 cut(s) 123, 154
StyD4I CCNGG 2 cut(s) 127, 198
TaqI TCGA 1 cut(s) 178
TasI AATT 2 cut(s) 123, 154
TatI WGTACW 1 cut(s) 90
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TseI GCWGC 1 cut(s) 135
TspDTI ATGAA 1 cut(s) 135
TspGWI ACGGA 1 cut(s) 117
XapI RAATTY 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.