Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0006496

Physical Location & Seq

Genomic Coordinates Reverse (-)

1292359 .. 1292753

Gene Structure 395 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G04735.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 318 bp

ATGAGCAATATGAAAATAACAAATAGGTTTATGCTAGTGGCAACATTCATAGCATGTGTGTTCATAAGCAGCATGAACATGACGGTTGGAAAAGTAATTGGGTATCCAGGGTTGAAGCCTGATCTTCCTTGTGATCATCATCGGTATCCTTCTGCATGTGCACCCTCCGAACAACCAGTGAATCCTTACAGACGGGGATGTAGTAAAATACATAGATGTAGACGCGATTCACCCCCTGCACCAATTTCTAGAAAAATGTTAATACGAGGTCAATTAATTTATAATAATGCATATAATGCATATATCCAATATCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005102 GO:0005179 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005911 GO:0007154 GO:0007165 GO:0007267 GO:0008150 GO:0009506 GO:0009966 GO:0009987 GO:0010469 GO:0010646 GO:0019722 GO:0019932 GO:0023051 GO:0023052 GO:0030054 GO:0030545 GO:0035556 GO:0048018 GO:0048046 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0065009 GO:0098772

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RALF

Protein Analysis

105

Amino Acids

11.95

Weight (kDa)

9.8

Isoelectric Point (pI)

58.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RALF	PF05498	31 - 75	1.6e-11	Rapid ALkalinization Factor (RALF)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006496)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23145 AT1G23147 AT1G60625 AT1G60815 AT1G60913 AT1G61563 AT1G61566 AT2G22055 AT2G32835 AT3G04735 AT4G11510
fragaria_vesca	FvH4_6g41850
prunus_persica	Prupe.3G207900_v2.0.a1
rosa_roxburghii	Rroxscaffold_2G00092030 Rroxscaffold_2G00092050 Rroxscaffold_2G00107720 Rroxscaffold_2G00107730
rosa_samantha	Rh2AG386600 Rh2AG386700 Rh2BG541700 Rh2CG373900 Rh2CG374000 Rh2DG409600
rosa_wichuraiana	Rw2G031640 Rw2G043630 Rw2G043650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	282
AccI	GTMKAC	1 cut(s)	220
AccII	CGCG	1 cut(s)	225
AgsI	TTSAA	1 cut(s)	115
AjnI	CCWGG	1 cut(s)	106
Alw21I	GWGCWC	1 cut(s)	163
Alw44I	GTGCAC	1 cut(s)	159
ApaLI	GTGCAC	1 cut(s)	159
ApeKI	GCWGC	1 cut(s)	69
AseI	ATTAAT	1 cut(s)	275
AsuHPI	GGTGA	1 cut(s)	222
BaeGI	GKGCMC	1 cut(s)	163
Bbv12I	GWGCWC	1 cut(s)	163
BbvI	GCAGC	1 cut(s)	81
BciT130I	CCWGG	1 cut(s)	108
BciVI	GTATCC	2 cut(s)	114, 156
BclI	TGATCA	1 cut(s)	133
BfaI	CTAG	2 cut(s)	35, 249
BfuI	GTATCC	2 cut(s)	114, 156
BisI	GCNGC	1 cut(s)	70
BlsI	GCNGC	1 cut(s)	71
Bme1390I	CCNGG	1 cut(s)	108
BmrFI	CCNGG	1 cut(s)	108
BsaBI	GATNNNNATC	1 cut(s)	138
BsaJI	CCNNGG	1 cut(s)	107
Bse1I	ACTGG	1 cut(s)	176
Bse8I	GATNNNNATC	1 cut(s)	138
BseBI	CCWGG	1 cut(s)	108
BseDI	CCNNGG	1 cut(s)	107
BseGI	GGATG	1 cut(s)	203
BseJI	GATNNNNATC	1 cut(s)	138
BseNI	ACTGG	1 cut(s)	176
BseSI	GKGCMC	1 cut(s)	163
BseXI	GCAGC	1 cut(s)	81
BsgI	GTGCAG	1 cut(s)	222
Bsh1236I	CGCG	1 cut(s)	225
BsiHKAI	GWGCWC	1 cut(s)	163
Bsp1286I	GDGCHC	1 cut(s)	163
Bsp143I	GATC	2 cut(s)	121, 133
BspFNI	CGCG	1 cut(s)	225
BsrI	ACTGG	1 cut(s)	176
BssECI	CCNNGG	1 cut(s)	107
BssMI	GATC	2 cut(s)	121, 133
Bst2UI	CCWGG	1 cut(s)	108
Bst4CI	ACNGT	1 cut(s)	85
BstAPI	GCANNNNNTGC	1 cut(s)	296
BstF5I	GGATG	1 cut(s)	203
BstFNI	CGCG	1 cut(s)	225
BstKTI	GATC	2 cut(s)	124, 136
BstMBI	GATC	2 cut(s)	121, 133
BstMWI	GCNNNNNNNGC	1 cut(s)	296
BstNI	CCWGG	1 cut(s)	108
BstNSI	RCATGY	2 cut(s)	57, 159
BstSCI	CCNGG	1 cut(s)	106
BstSLI	GKGCMC	1 cut(s)	163
BstUI	CGCG	1 cut(s)	225
BstV1I	GCAGC	1 cut(s)	81
BsuI	GTATCC	2 cut(s)	114, 156
BtsCI	GGATG	1 cut(s)	203
BtsIMutI	CAGTG	1 cut(s)	183
CseI	GACGC	1 cut(s)	231
CviAII	CATG	5 cut(s)	54, 73, 79, 156, 315
CviJI	RGCY	1 cut(s)	118
CviKI_1	RGCY	1 cut(s)	118
DpnI	GATC	2 cut(s)	123, 135
DpnII	GATC	2 cut(s)	121, 133
EcoRII	CCWGG	1 cut(s)	106
EcoT22I	ATGCAT	2 cut(s)	292, 301
FaeI	CATG	5 cut(s)	57, 76, 82, 159, 318
FatI	CATG	5 cut(s)	53, 72, 78, 155, 314
FbaI	TGATCA	1 cut(s)	133
FblI	GTMKAC	1 cut(s)	220
Fnu4HI	GCNGC	1 cut(s)	70
FokI	GGATG	1 cut(s)	210
Fsp4HI	GCNGC	1 cut(s)	70
FspBI	CTAG	2 cut(s)	35, 249
GluI	GCNGC	1 cut(s)	70
HgaI	GACGC	1 cut(s)	231
Hin1II	CATG	5 cut(s)	57, 76, 82, 159, 318
HinfI	GANTC	2 cut(s)	181, 227
HphI	GGTGA	1 cut(s)	222
Hpy166II	GTNNAC	2 cut(s)	161, 221
Hpy188I	TCNGA	1 cut(s)	169
Hpy188III	TCNNGA	1 cut(s)	249
Hpy8I	GTNNAC	2 cut(s)	161, 221
HpyAV	CCTTC	1 cut(s)	159
HpyCH4III	ACNGT	1 cut(s)	85
HpyCH4V	TGCA	5 cut(s)	155, 161, 239, 290, 299
HpyF10VI	GCNNNNNNNGC	1 cut(s)	296
Hsp92II	CATG	5 cut(s)	57, 76, 82, 159, 318
Ksp22I	TGATCA	1 cut(s)	133
Kzo9I	GATC	2 cut(s)	121, 133
LpnPI	CCDG	5 cut(s)	93, 120, 132, 189, 249
Lsp1109I	GCAGC	1 cut(s)	81
MaeI	CTAG	2 cut(s)	35, 249
MalI	GATC	2 cut(s)	123, 135
MboI	GATC	2 cut(s)	121, 133
MboII	GAAGA	1 cut(s)	116
MhlI	GDGCHC	1 cut(s)	163
MluCI	AATT	4 cut(s)	96, 243, 272, 276
MmeI	TCCRAC	1 cut(s)	67
MnlI	CCTC	2 cut(s)	175, 260
Mph1103I	ATGCAT	2 cut(s)	292, 301
MseI	TTAA	2 cut(s)	260, 275
MslI	CAYNNNNRTG	1 cut(s)	77
MspR9I	CCNGG	1 cut(s)	108
MvaI	CCWGG	1 cut(s)	108
MvnI	CGCG	1 cut(s)	225
MwoI	GCNNNNNNNGC	1 cut(s)	296
NdeII	GATC	2 cut(s)	121, 133
NlaIII	CATG	5 cut(s)	57, 76, 82, 159, 318
NsiI	ATGCAT	2 cut(s)	292, 301
NspI	RCATGY	2 cut(s)	57, 159
PfeI	GAWTC	2 cut(s)	181, 227
PkrI	GCNGC	1 cut(s)	71
PshBI	ATTAAT	1 cut(s)	275
PsiI	TTATAA	1 cut(s)	282
Psp6I	CCWGG	1 cut(s)	106
PspGI	CCWGG	1 cut(s)	106
RseI	CAYNNNNRTG	1 cut(s)	77
SaqAI	TTAA	2 cut(s)	260, 275
SatI	GCNGC	1 cut(s)	70
Sau3AI	GATC	2 cut(s)	121, 133
ScrFI	CCNGG	1 cut(s)	108
SduI	GDGCHC	1 cut(s)	163
SetI	ASST	2 cut(s)	29, 271
SmiMI	CAYNNNNRTG	1 cut(s)	77
Sse9I	AATT	4 cut(s)	96, 243, 272, 276
SspMI	CTAG	2 cut(s)	35, 249
StyD4I	CCNGG	1 cut(s)	106
TaaI	ACNGT	1 cut(s)	85
TasI	AATT	4 cut(s)	96, 243, 272, 276
TfiI	GAWTC	2 cut(s)	181, 227
Tru1I	TTAA	2 cut(s)	260, 275
Tru9I	TTAA	2 cut(s)	260, 275
TscAI	CASTG	1 cut(s)	183
TseI	GCWGC	1 cut(s)	69
TspDTI	ATGAA	4 cut(s)	26, 37, 52, 89
TspRI	CASTG	1 cut(s)	183
VneI	GTGCAC	1 cut(s)	159
VspI	ATTAAT	1 cut(s)	275
XbaI	TCTAGA	1 cut(s)	248
XceI	RCATGY	2 cut(s)	57, 159
XmiI	GTMKAC	1 cut(s)	220
XspI	CTAG	2 cut(s)	35, 249
Zsp2I	ATGCAT	2 cut(s)	292, 301

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.