Rroxscaffold_2G00107720

Cell signaling peptide that may regulate plant stress, growth, and development. Mediates a rapid alkalinization of extracellular space by mediating a transient increase in the cytoplasmic Ca(2 ) concentration leading to a calcium-dependent signaling events through a cell surface receptor and a concomitant activation of some intracellular mitogen-activated protein kinases (By similarity)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006496
Physical Location & Seq
Reverse (-)
31707919 .. 31708158
240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00107720.1

Sequence Viewer

Length: 240 bp
ATGGCGGTTGTGTTCAAGACAATGAGTGTTTTGTGCCTAATTATGTTTGCATTAGTTTTGATTGAAGAAGTAGATAGCACACGGTTTATACACTACGGACCTATTCAAAGAGGTGATTCCACTCCTGGATGCAGCCCAAAGTATCCACAGAATTGCAACCATGGAGATGCAAATCATTATCAAAGAGGTTGTACCCCGCAATTGGGATGCAGGGAAGGTCCCTCGAAACCTGAGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

79

Amino Acids

8.74

Weight (kDa)

6.8

Isoelectric Point (pI)

57.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 25 - 71 4e-06 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 5, 197
AfaI GTAC 1 cut(s) 193
AfiI CCNNNNNNNGG 2 cut(s) 202, 203
AgsI TTSAA 3 cut(s) 16, 65, 107
AjnI CCWGG 1 cut(s) 124
ApeKI GCWGC 1 cut(s) 132
AspS9I GGNCC 2 cut(s) 98, 218
AsuHPI GGTGA 1 cut(s) 125
AvaII GGWCC 2 cut(s) 98, 218
AxyI CCTNAGG 1 cut(s) 231
BbvI GCAGC 1 cut(s) 144
BciT130I CCWGG 1 cut(s) 126
BciVI GTATCC 1 cut(s) 153
BfuI GTATCC 1 cut(s) 153
BisI GCNGC 1 cut(s) 133
BlsI GCNGC 1 cut(s) 134
Bme1390I CCNGG 1 cut(s) 126
Bme18I GGWCC 2 cut(s) 98, 218
BmgT120I GGNCC 2 cut(s) 98, 218
BmiI GGNNCC 1 cut(s) 220
BmrFI CCNGG 1 cut(s) 126
BmsI GCATC 3 cut(s) 119, 157, 197
BsaBI GATNNNNATC 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 160
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 203
Bse21I CCTNAGG 1 cut(s) 231
Bse8I GATNNNNATC 1 cut(s) 171
BseBI CCWGG 1 cut(s) 126
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 2 cut(s) 134, 212
BseJI GATNNNNATC 1 cut(s) 171
BseLI CCNNNNNNNGG 2 cut(s) 202, 203
BseMII CTCAG 1 cut(s) 222
BseXI GCAGC 1 cut(s) 144
BslFI GGGAC 1 cut(s) 204
BslI CCNNNNNNNGG 2 cut(s) 202, 203
BsmFI GGGAC 1 cut(s) 204
Bsp19I CCATGG 1 cut(s) 160
BspACI CCGC 2 cut(s) 5, 197
BspCNI CTCAG 1 cut(s) 223
BspLI GGNNCC 1 cut(s) 220
BssECI CCNNGG 1 cut(s) 160
BssT1I CCWWGG 1 cut(s) 160
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 1 cut(s) 84
BstDEI CTNAG 1 cut(s) 231
BstDSI CCRYGG 1 cut(s) 160
BstF5I GGATG 2 cut(s) 134, 212
BstNI CCWGG 1 cut(s) 126
BstSCI CCNGG 1 cut(s) 124
BstV1I GCAGC 1 cut(s) 144
Bsu36I CCTNAGG 1 cut(s) 231
BsuI GTATCC 1 cut(s) 153
BtgI CCRYGG 1 cut(s) 160
BtsCI GGATG 2 cut(s) 134, 212
Cfr13I GGNCC 2 cut(s) 98, 218
Csp6I GTAC 1 cut(s) 192
CviAII CATG 1 cut(s) 161
CviJI RGCY 1 cut(s) 135
CviKI_1 RGCY 1 cut(s) 135
CviQI GTAC 1 cut(s) 192
DdeI CTNAG 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 160
Eco47I GGWCC 2 cut(s) 98, 218
Eco81I CCTNAGG 1 cut(s) 231
EcoO109I RGGNCCY 1 cut(s) 218
EcoRII CCWGG 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 160
FaeI CATG 1 cut(s) 164
FaiI YATR 3 cut(s) 44, 89, 162
FaqI GGGAC 1 cut(s) 204
FatI CATG 1 cut(s) 160
FauI CCCGC 1 cut(s) 204
Fnu4HI GCNGC 1 cut(s) 133
FokI GGATG 2 cut(s) 141, 219
Fsp4HI GCNGC 1 cut(s) 133
GluI GCNGC 1 cut(s) 133
Hin1II CATG 1 cut(s) 164
HinfI GANTC 1 cut(s) 116
HphI GGTGA 1 cut(s) 125
Hpy188III TCNNGA 1 cut(s) 16
HpyAV CCTTC 1 cut(s) 209
HpyCH4III ACNGT 1 cut(s) 84
HpyCH4V TGCA 5 cut(s) 50, 132, 156, 170, 210
HpyF3I CTNAG 1 cut(s) 231
Hsp92II CATG 1 cut(s) 164
LpnPI CCDG 3 cut(s) 111, 138, 196
Lsp1109I GCAGC 1 cut(s) 144
LweI GCATC 3 cut(s) 119, 157, 197
MboII GAAGA 1 cut(s) 77
MfeI CAATTG 1 cut(s) 200
MluCI AATT 3 cut(s) 39, 151, 200
MnlI CCTC 4 cut(s) 104, 179, 226, 232
MslI CAYNNNNRTG 1 cut(s) 165
MspR9I CCNGG 1 cut(s) 126
MunI CAATTG 1 cut(s) 200
MvaI CCWGG 1 cut(s) 126
NcoI CCATGG 1 cut(s) 160
NlaIII CATG 1 cut(s) 164
NlaIV GGNNCC 1 cut(s) 220
PfeI GAWTC 1 cut(s) 116
PfoI TCCNGGA 1 cut(s) 124
PkrI GCNGC 1 cut(s) 134
PpuMI RGGWCCY 1 cut(s) 218
Psp5II RGGWCCY 1 cut(s) 218
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 1 cut(s) 220
PspPI GGNCC 2 cut(s) 98, 218
PspPPI RGGWCCY 1 cut(s) 218
RsaI GTAC 1 cut(s) 193
RsaNI GTAC 1 cut(s) 192
RseI CAYNNNNRTG 1 cut(s) 165
SatI GCNGC 1 cut(s) 133
Sau96I GGNCC 2 cut(s) 98, 218
ScrFI CCNGG 1 cut(s) 126
SetI ASST 6 cut(s) 103, 115, 190, 220, 232, 237
SfaNI GCATC 3 cut(s) 119, 157, 197
SgeI CNNG 8 cut(s) 28, 93, 137, 138, 173, 208, 223, 235
SinI GGWCC 2 cut(s) 98, 218
SmiMI CAYNNNNRTG 1 cut(s) 165
Sse9I AATT 3 cut(s) 39, 151, 200
SsiI CCGC 2 cut(s) 5, 197
StyD4I CCNGG 1 cut(s) 124
StyI CCWWGG 1 cut(s) 160
TaaI ACNGT 1 cut(s) 84
TaqI TCGA 1 cut(s) 224
TasI AATT 3 cut(s) 39, 151, 200
TfiI GAWTC 1 cut(s) 116
TseI GCWGC 1 cut(s) 132
TspGWI ACGGA 1 cut(s) 111
VpaK11BI GGWCC 2 cut(s) 98, 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.