AT1G32350

Belongs to the alternative oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006091
Physical Location & Seq
Reverse (-)
11666886 .. 11668690
1805 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G32350.1

Sequence Viewer

Length: 957 bp
ATGTCCTACAGATCGATTTACCGCACTCTTCGACCGGTACTATCATCTTCGGTACAATCCTCCGGTTTAGGAATTGGAGGATTCAGGGGACATCTCATTAGCCACTTGCCCAATGTTCGGCTATTGAGCTCTGACACGTCATCTCCGGTAAGTGGGAATAACCAGCCAGAAAATCCTATCCGAACGGCCGATGGTAAAGTTATATCCACTTATTGGGGTATACCTCCTACTAAGATCACTAAACCGGACGGTTCAGCTTGGAAGTGGAATTGTTTTCAGCCATGGGATTCATACAAACCGGATGTGTCCATTGATGTAACTAAACATCATAAACCCTCCAATTTCACTGACAAATTCGCATATTGGACCGTTCAAACTCTGAAAATACCGGTTCAACTATTTTTTCAGAGGAAGCACATGTGCCATGCGATGTTGCTAGAGACGGTGGCTGCGGTGCCGGGAATGGTCGGGGGGATGCTTTTGCACTTGAAATCTCTCCGGAGGTTCGAACATAGCGGGGGATGGATCAAAGCTTTGCTCGAAGAGGCTGAGAACGAGCGTATGCATCTCATGACTTTCATCGAACTTTCACAACCCAAATGGTACGAACGAGCGATTGTGTTCACGGTCCAAGGCGTTTTCTTCAACGCATATTTCCTGGCTTATGTAATTTCACCCAAACTTGCTCATCGTATCACTGGATACTTAGAAGAAGAGGCTGTAAATTCTTACACTGAATTTCTCAAAGACATTGATGCCGGAAAATTCGAAAACTCGCCAGCTCCAGCCATCGCAATCGATTACTGGCGGTTGCCTAAAGATGCAACGCTTAGGGATGTGGTTTATGTTATACGAGCTGACGAAGCTCACCACCGTGATATTAACCACTATGCTTCGGATATACAATTCAAAGGACATGAACTCAAGGAAGCTCCGGCTCCTATTGGATATCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AOX

Protein Analysis

318

Amino Acids

36.2

Weight (kDa)

8.65

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 107 - 299 1.9e-82 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 454
AccB7I CCANNNNNTGG 1 cut(s) 213
AccI GTMKAC 1 cut(s) 220
AccIII TCCGGA 1 cut(s) 498
AciI CCGC 4 cut(s) 22, 452, 516, 808
AclWI GGATC 1 cut(s) 533
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 4 cut(s) 353, 724, 737, 764
AfaI GTAC 3 cut(s) 39, 54, 605
AfiI CCNNNNNNNGG 3 cut(s) 117, 152, 213
AflIII ACRYGT 2 cut(s) 135, 417
AgeI ACCGGT 2 cut(s) 34, 388
AgsI TTSAA 5 cut(s) 374, 395, 490, 646, 910
AjiI CACGTC 1 cut(s) 138
AjnI CCWGG 1 cut(s) 657
AleI CACNNNNGTG 1 cut(s) 873
AluBI AGCT 7 cut(s) 129, 257, 533, 782, 857, 866, 932
AluI AGCT 7 cut(s) 129, 257, 533, 782, 857, 866, 932
Alw21I GWGCWC 1 cut(s) 131
Alw26I GTCTC 1 cut(s) 434
AlwI GGATC 1 cut(s) 533
Aor13HI TCCGGA 1 cut(s) 498
AoxI GGCC 1 cut(s) 186
ApeKI GCWGC 1 cut(s) 449
ApoI RAATTY 4 cut(s) 353, 724, 737, 764
AsiGI ACCGGT 2 cut(s) 34, 388
AspS9I GGNCC 2 cut(s) 366, 628
AsuC2I CCSGG 1 cut(s) 459
AsuHPI GGTGA 2 cut(s) 666, 860
AsuII TTCGAA 2 cut(s) 507, 768
AvaII GGWCC 2 cut(s) 366, 628
BanI GGYRCC 1 cut(s) 454
BanII GRGCYC 1 cut(s) 131
Bbv12I GWGCWC 1 cut(s) 131
BbvI GCAGC 1 cut(s) 436
BccI CCATC 3 cut(s) 185, 516, 797
BceAI ACGGC 1 cut(s) 201
BciT130I CCWGG 1 cut(s) 659
BciVI GTATCC 1 cut(s) 695
BcnI CCSGG 1 cut(s) 459
BcoDI GTCTC 1 cut(s) 434
BfaI CTAG 1 cut(s) 437
BfmI CTRYAG 1 cut(s) 7
BfuI GTATCC 1 cut(s) 695
BisI GCNGC 1 cut(s) 450
BlsI GCNGC 1 cut(s) 451
Bme1390I CCNGG 2 cut(s) 459, 659
Bme18I GGWCC 2 cut(s) 366, 628
BmgBI CACGTC 1 cut(s) 138
BmgT120I GGNCC 2 cut(s) 366, 628
BmiI GGNNCC 2 cut(s) 456, 939
BmrFI CCNGG 2 cut(s) 459, 659
BmsI GCATC 4 cut(s) 465, 574, 745, 811
BpmI CTGGAG 1 cut(s) 768
Bpu10I CCTNAGC 1 cut(s) 830
Bpu14I TTCGAA 2 cut(s) 507, 768
BpuEI CTTGAG 1 cut(s) 908
BpuMI CCSGG 1 cut(s) 459
Bsa29I ATCGAT 2 cut(s) 14, 798
BsaJI CCNNGG 2 cut(s) 281, 631
BsaWI WCCGGW 7 cut(s) 34, 62, 145, 244, 298, 388, 498
BsaXI ACNNNNNCTCC 2 cut(s) 127, 157
Bsc4I CCNNNNNNNGG 3 cut(s) 117, 152, 213
Bse118I RCCGGY 2 cut(s) 34, 388
Bse1I ACTGG 2 cut(s) 703, 809
BseAI TCCGGA 1 cut(s) 498
BseBI CCWGG 1 cut(s) 659
BseCI ATCGAT 2 cut(s) 14, 798
BseDI CCNNGG 2 cut(s) 281, 631
BseGI GGATG 4 cut(s) 307, 480, 527, 841
BseLI CCNNNNNNNGG 3 cut(s) 117, 152, 213
BseMII CTCAG 1 cut(s) 540
BseNI ACTGG 2 cut(s) 703, 809
BseX3I CGGCCG 1 cut(s) 186
BseXI GCAGC 1 cut(s) 436
Bsh1285I CGRYCG 2 cut(s) 35, 189
BshFI GGCC 1 cut(s) 188
BshNI GGYRCC 1 cut(s) 454
BshTI ACCGGT 2 cut(s) 34, 388
BshVI ATCGAT 2 cut(s) 14, 798
BsiEI CGRYCG 2 cut(s) 35, 189
BsiHKAI GWGCWC 1 cut(s) 131
BslFI GGGAC 1 cut(s) 102
BslI CCNNNNNNNGG 3 cut(s) 117, 152, 213
BsmAI GTCTC 1 cut(s) 434
BsmBI CGTCTC 1 cut(s) 434
BsmFI GGGAC 1 cut(s) 102
BsnI GGCC 1 cut(s) 188
Bsp119I TTCGAA 2 cut(s) 507, 768
Bsp1286I GDGCHC 1 cut(s) 131
Bsp13I TCCGGA 1 cut(s) 498
Bsp143I GATC 3 cut(s) 11, 234, 525
Bsp19I CCATGG 1 cut(s) 281
BspACI CCGC 4 cut(s) 22, 452, 516, 808
BspANI GGCC 1 cut(s) 188
BspCNI CTCAG 1 cut(s) 541
BspDI ATCGAT 2 cut(s) 14, 798
BspEI TCCGGA 1 cut(s) 498
BspHI TCATGA 1 cut(s) 570
BspLI GGNNCC 2 cut(s) 456, 939
BspPI GGATC 1 cut(s) 533
BspT104I TTCGAA 2 cut(s) 507, 768
BspT107I GGYRCC 1 cut(s) 454
BsrFI RCCGGY 2 cut(s) 34, 388
BsrI ACTGG 2 cut(s) 703, 809
BssAI RCCGGY 2 cut(s) 34, 388
BssECI CCNNGG 2 cut(s) 281, 631
BssMI GATC 3 cut(s) 11, 234, 525
BssNAI GTATAC 1 cut(s) 221
BssT1I CCWWGG 2 cut(s) 281, 631
Bst1107I GTATAC 1 cut(s) 221
Bst2UI CCWGG 1 cut(s) 659
Bst4CI ACNGT 5 cut(s) 251, 370, 445, 628, 875
Bst6I CTCTTC 3 cut(s) 33, 537, 708
BstBI TTCGAA 2 cut(s) 507, 768
BstC8I GCNNGC 1 cut(s) 780
BstDEI CTNAG 4 cut(s) 231, 549, 706, 830
BstDSI CCRYGG 1 cut(s) 281
BstF5I GGATG 4 cut(s) 307, 480, 527, 841
BstKTI GATC 3 cut(s) 14, 237, 528
BstMAI GTCTC 1 cut(s) 434
BstMBI GATC 3 cut(s) 11, 234, 525
BstMCI CGRYCG 2 cut(s) 35, 189
BstMWI GCNNNNNNNGC 1 cut(s) 863
BstNI CCWGG 1 cut(s) 659
BstNSI RCATGY 1 cut(s) 421
BstSCI CCNGG 2 cut(s) 457, 657
BstSFI CTRYAG 1 cut(s) 7
BstV1I GCAGC 1 cut(s) 436
BstZ17I GTATAC 1 cut(s) 221
BstZI CGGCCG 1 cut(s) 186
Bsu15I ATCGAT 2 cut(s) 14, 798
BsuI GTATCC 1 cut(s) 695
BsuRI GGCC 1 cut(s) 188
BsuTUI ATCGAT 2 cut(s) 14, 798
BtgI CCRYGG 1 cut(s) 281
BtgZI GCGATG 2 cut(s) 443, 775
BtrI CACGTC 1 cut(s) 138
BtsCI GGATG 4 cut(s) 307, 480, 527, 841
BtsIMutI CAGTG 3 cut(s) 345, 696, 732
Cac8I GCNNGC 1 cut(s) 780
CciI TCATGA 1 cut(s) 570
Cfr10I RCCGGY 2 cut(s) 34, 388
Cfr13I GGNCC 2 cut(s) 366, 628
ClaI ATCGAT 2 cut(s) 14, 798
Csp6I GTAC 3 cut(s) 38, 53, 604
CspAI ACCGGT 2 cut(s) 34, 388
CviAII CATG 5 cut(s) 282, 418, 425, 571, 917
CviQI GTAC 3 cut(s) 38, 53, 604
DdeI CTNAG 4 cut(s) 231, 549, 706, 830
DpnI GATC 3 cut(s) 13, 236, 527
DpnII GATC 3 cut(s) 11, 234, 525
EaeI YGGCCR 1 cut(s) 186
EagI CGGCCG 1 cut(s) 186
Eam1104I CTCTTC 3 cut(s) 33, 537, 708
EarI CTCTTC 3 cut(s) 33, 537, 708
Ecl136II GAGCTC 1 cut(s) 129
EclXI CGGCCG 1 cut(s) 186
Eco130I CCWWGG 2 cut(s) 281, 631
Eco24I GRGCYC 1 cut(s) 131
Eco32I GATATC 1 cut(s) 950
Eco47I GGWCC 2 cut(s) 366, 628
Eco52I CGGCCG 1 cut(s) 186
Eco53kI GAGCTC 1 cut(s) 129
EcoICRI GAGCTC 1 cut(s) 129
EcoRII CCWGG 1 cut(s) 657
EcoRV GATATC 1 cut(s) 950
EcoT14I CCWWGG 2 cut(s) 281, 631
EcoT22I ATGCAT 1 cut(s) 567
EcoT38I GRGCYC 1 cut(s) 131
ErhI CCWWGG 2 cut(s) 281, 631
Esp3I CGTCTC 1 cut(s) 434
FaeI CATG 5 cut(s) 285, 421, 428, 574, 920
FaqI GGGAC 1 cut(s) 102
FatI CATG 5 cut(s) 281, 417, 424, 570, 916
FauI CCCGC 1 cut(s) 509
FblI GTMKAC 1 cut(s) 220
Fnu4HI GCNGC 1 cut(s) 450
FokI GGATG 4 cut(s) 314, 487, 534, 848
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 1 cut(s) 450
FspBI CTAG 1 cut(s) 437
GluI GCNGC 1 cut(s) 450
GsuI CTGGAG 1 cut(s) 768
HaeIII GGCC 1 cut(s) 188
Hin1II CATG 5 cut(s) 285, 421, 428, 574, 920
HindIII AAGCTT 1 cut(s) 531
HinfI GANTC 2 cut(s) 81, 287
HphI GGTGA 2 cut(s) 666, 860
Hpy166II GTNNAC 2 cut(s) 221, 624
Hpy188I TCNGA 5 cut(s) 133, 182, 381, 408, 898
Hpy188III TCNNGA 2 cut(s) 499, 571
Hpy8I GTNNAC 2 cut(s) 221, 624
HpyCH4III ACNGT 5 cut(s) 251, 370, 445, 628, 875
HpyCH4IV ACGT 1 cut(s) 137
HpyCH4V TGCA 3 cut(s) 484, 565, 824
HpyF10VI GCNNNNNNNGC 1 cut(s) 863
HpyF3I CTNAG 4 cut(s) 231, 549, 706, 830
HpySE526I ACGT 1 cut(s) 137
Hsp92II CATG 5 cut(s) 285, 421, 428, 574, 920
Kpn2I TCCGGA 1 cut(s) 498
Kzo9I GATC 3 cut(s) 11, 234, 525
LmnI GCTCC 3 cut(s) 787, 937, 943
Lsp1109I GCAGC 1 cut(s) 436
LweI GCATC 4 cut(s) 465, 574, 745, 811
MaeI CTAG 1 cut(s) 437
MaeII ACGT 1 cut(s) 137
MaeIII GTNAC 1 cut(s) 316
MalI GATC 3 cut(s) 13, 236, 527
MboI GATC 3 cut(s) 11, 234, 525
MboII GAAGA 6 cut(s) 20, 39, 554, 634, 722, 725
MhlI GDGCHC 1 cut(s) 131
MluCI AATT 9 cut(s) 72, 268, 340, 353, 669, 724, 737, 764, 905
MnlI CCTC 8 cut(s) 70, 71, 234, 346, 402, 495, 538, 709
Mph1103I ATGCAT 1 cut(s) 567
MroI TCCGGA 1 cut(s) 498
MseI TTAA 2 cut(s) 882, 955
MslI CAYNNNNRTG 1 cut(s) 873
MspR9I CCNGG 2 cut(s) 459, 659
MvaI CCWGG 1 cut(s) 659
MwoI GCNNNNNNNGC 1 cut(s) 863
NciI CCSGG 1 cut(s) 459
NcoI CCATGG 1 cut(s) 281
NdeII GATC 3 cut(s) 11, 234, 525
NlaIII CATG 5 cut(s) 285, 421, 428, 574, 920
NlaIV GGNNCC 2 cut(s) 456, 939
NsiI ATGCAT 1 cut(s) 567
NspI RCATGY 1 cut(s) 421
NspV TTCGAA 2 cut(s) 507, 768
OliI CACNNNNGTG 1 cut(s) 873
PagI TCATGA 1 cut(s) 570
PciI ACATGT 1 cut(s) 417
PfeI GAWTC 2 cut(s) 81, 287
PflMI CCANNNNNTGG 1 cut(s) 213
PinAI ACCGGT 2 cut(s) 34, 388
PkrI GCNGC 1 cut(s) 451
PscI ACATGT 1 cut(s) 417
Psp124BI GAGCTC 1 cut(s) 131
Psp6I CCWGG 1 cut(s) 657
PspGI CCWGG 1 cut(s) 657
PspN4I GGNNCC 2 cut(s) 456, 939
PspPI GGNCC 2 cut(s) 366, 628
RsaI GTAC 3 cut(s) 39, 54, 605
RsaNI GTAC 3 cut(s) 38, 53, 604
RseI CAYNNNNRTG 1 cut(s) 873
SacI GAGCTC 1 cut(s) 131
SaqAI TTAA 2 cut(s) 882, 955
SatI GCNGC 1 cut(s) 450
Sau3AI GATC 3 cut(s) 11, 234, 525
Sau96I GGNCC 2 cut(s) 366, 628
ScrFI CCNGG 2 cut(s) 459, 659
SduI GDGCHC 1 cut(s) 131
SfaNI GCATC 4 cut(s) 465, 574, 745, 811
SfcI CTRYAG 1 cut(s) 7
SfuI TTCGAA 2 cut(s) 507, 768
SinI GGWCC 2 cut(s) 366, 628
SmiMI CAYNNNNRTG 1 cut(s) 873
SmlI CTYRAG 1 cut(s) 923
SmoI CTYRAG 1 cut(s) 923
Sse9I AATT 9 cut(s) 72, 268, 340, 353, 669, 724, 737, 764, 905
SsiI CCGC 4 cut(s) 22, 452, 516, 808
SspMI CTAG 1 cut(s) 437
SstI GAGCTC 1 cut(s) 131
StyD4I CCNGG 2 cut(s) 457, 657
StyI CCWWGG 2 cut(s) 281, 631
TaaI ACNGT 5 cut(s) 251, 370, 445, 628, 875
TaiI ACGT 1 cut(s) 140
TaqI TCGA 7 cut(s) 14, 31, 507, 540, 582, 768, 798
TasI AATT 9 cut(s) 72, 268, 340, 353, 669, 724, 737, 764, 905
TfiI GAWTC 2 cut(s) 81, 287
Tru1I TTAA 2 cut(s) 882, 955
Tru9I TTAA 2 cut(s) 882, 955
TscAI CASTG 3 cut(s) 352, 703, 739
TseI GCWGC 1 cut(s) 449
TspDTI ATGAA 3 cut(s) 279, 568, 933
TspRI CASTG 3 cut(s) 352, 703, 739
Van91I CCANNNNNTGG 1 cut(s) 213
VpaK11BI GGWCC 2 cut(s) 366, 628
XapI RAATTY 4 cut(s) 353, 724, 737, 764
XceI RCATGY 1 cut(s) 421
XmiI GTMKAC 1 cut(s) 220
XspI CTAG 1 cut(s) 437
Zsp2I ATGCAT 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.