Rroxscaffold_3G00245670

Belongs to the alternative oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006091
Physical Location & Seq
Forward (+)
38741355 .. 38741846
492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00245670.1

Sequence Viewer

Length: 492 bp
ATGATGCTGGAGACAGTGGCAGCAGTGCCTGGAATGGTAGGAGGGATGCTGCTGCACTGCAAATCGCTTAGGCGATTCGAGCACAGTGGCGGATGGATCAAAGCTCTTCTAGAAGAAGCCGAGAACGAGCGTATGCACCTCATGATCTTCATGGAAGTCGCCAAGCCCAGGTGGTACGAGCGTGCACTTGTATTTGCCGTTCAAGGTGTTTTCTTCAATGCGTACTTCTTGGGATATATGATTTCACCCAAGTTTGCTCATAGAATGGTTGGATATCTTGAGGAGGAAGCAATTCACTCATATACCGAGTTCCTCAAGGAATTAGACAAAGGTAACATTGAGAATGTTCCAGCTCCAGCTATTGCTATTGATTATTGGCAGCTACCTTCCAATTCCACCTTGAGAGATGTTGTTACAGTTGTTCGGGCAGACGAGGCTCACCACCGAGATGTCAATCACTTCGCTTCGGTAAGGACTGAAGATCGCACCTGA

Protein Analysis

163

Amino Acids

18.76

Weight (kDa)

5.89

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 1 - 154 1.7e-74 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 90
AclWI GGATC 1 cut(s) 104
AfaI GTAC 2 cut(s) 176, 224
AfiI CCNNNNNNNGG 1 cut(s) 168
AgsI TTSAA 2 cut(s) 203, 217
AjnI CCWGG 2 cut(s) 28, 167
AluBI AGCT 4 cut(s) 104, 353, 359, 382
AluI AGCT 4 cut(s) 104, 353, 359, 382
Alw21I GWGCWC 2 cut(s) 84, 187
Alw26I GTCTC 1 cut(s) 5
Alw44I GTGCAC 1 cut(s) 183
AlwI GGATC 1 cut(s) 104
AlwNI CAGNNNCTG 1 cut(s) 29
ApaLI GTGCAC 1 cut(s) 183
ApeKI GCWGC 4 cut(s) 20, 49, 52, 379
Asp700I GAANNNNTTC 1 cut(s) 291
AsuHPI GGTGA 2 cut(s) 237, 431
BaeGI GKGCMC 1 cut(s) 187
BaeI ACNNNNGTAYC 2 cut(s) 166, 199
Bbv12I GWGCWC 2 cut(s) 84, 187
BbvI GCAGC 4 cut(s) 32, 36, 39, 391
BccI CCATC 1 cut(s) 87
BceAI ACGGC 1 cut(s) 182
BciT130I CCWGG 2 cut(s) 30, 169
BcoDI GTCTC 1 cut(s) 5
BfaI CTAG 1 cut(s) 110
BisI GCNGC 4 cut(s) 21, 50, 53, 380
BlsI GCNGC 4 cut(s) 22, 51, 54, 381
Bme1390I CCNGG 2 cut(s) 30, 169
BmrFI CCNGG 2 cut(s) 30, 169
BmsI GCATC 1 cut(s) 36
BpmI CTGGAG 2 cut(s) 29, 339
Bpu10I CCTNAGC 1 cut(s) 68
BpuEI CTTGAG 3 cut(s) 299, 299, 421
BsaBI GATNNNNATC 1 cut(s) 453
BsaJI CCNNGG 1 cut(s) 167
Bsc4I CCNNNNNNNGG 1 cut(s) 168
Bse8I GATNNNNATC 1 cut(s) 453
BseBI CCWGG 2 cut(s) 30, 169
BseDI CCNNGG 1 cut(s) 167
BseGI GGATG 2 cut(s) 51, 98
BseJI GATNNNNATC 1 cut(s) 453
BseLI CCNNNNNNNGG 1 cut(s) 168
BseRI GAGGAG 1 cut(s) 296
BseSI GKGCMC 1 cut(s) 187
BseXI GCAGC 4 cut(s) 32, 36, 39, 391
BsgI GTGCAG 1 cut(s) 38
BsiHKAI GWGCWC 2 cut(s) 84, 187
BslI CCNNNNNNNGG 1 cut(s) 168
BsmAI GTCTC 1 cut(s) 5
Bsp1286I GDGCHC 2 cut(s) 84, 187
Bsp143I GATC 3 cut(s) 96, 144, 481
BspACI CCGC 1 cut(s) 90
BspHI TCATGA 1 cut(s) 141
BspPI GGATC 1 cut(s) 104
BspQI GCTCTTC 1 cut(s) 111
BssECI CCNNGG 1 cut(s) 167
BssMI GATC 3 cut(s) 96, 144, 481
Bst2UI CCWGG 2 cut(s) 30, 169
Bst4CI ACNGT 3 cut(s) 16, 86, 418
Bst6I CTCTTC 1 cut(s) 111
BstC8I GCNNGC 1 cut(s) 183
BstDEI CTNAG 1 cut(s) 68
BstF5I GGATG 2 cut(s) 51, 98
BstKTI GATC 3 cut(s) 99, 147, 484
BstMAI GTCTC 1 cut(s) 5
BstMBI GATC 3 cut(s) 96, 144, 481
BstMWI GCNNNNNNNGC 2 cut(s) 79, 434
BstNI CCWGG 2 cut(s) 30, 169
BstSCI CCNGG 2 cut(s) 28, 167
BstSLI GKGCMC 1 cut(s) 187
BstV1I GCAGC 4 cut(s) 32, 36, 39, 391
BtsCI GGATG 2 cut(s) 51, 98
BtsI GCAGTG 2 cut(s) 30, 55
BtsIMutI CAGTG 4 cut(s) 21, 30, 55, 91
Cac8I GCNNGC 1 cut(s) 183
CaiI CAGNNNCTG 1 cut(s) 29
CciI TCATGA 1 cut(s) 141
Csp6I GTAC 2 cut(s) 175, 223
CviAII CATG 2 cut(s) 142, 151
CviJI RGCY 7 cut(s) 104, 119, 166, 353, 359, 382, 437
CviKI_1 RGCY 7 cut(s) 104, 119, 166, 353, 359, 382, 437
CviQI GTAC 2 cut(s) 175, 223
DdeI CTNAG 1 cut(s) 68
DpnI GATC 3 cut(s) 98, 146, 483
DpnII GATC 3 cut(s) 96, 144, 481
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
EciI GGCGGA 1 cut(s) 105
Eco32I GATATC 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 28, 167
EcoRV GATATC 1 cut(s) 275
FaeI CATG 2 cut(s) 145, 154
FaiI YATR 8 cut(s) 134, 143, 152, 237, 239, 261, 301, 303
FatI CATG 2 cut(s) 141, 150
Fnu4HI GCNGC 4 cut(s) 21, 50, 53, 380
FokI GGATG 2 cut(s) 58, 105
Fsp4HI GCNGC 4 cut(s) 21, 50, 53, 380
FspBI CTAG 1 cut(s) 110
GluI GCNGC 4 cut(s) 21, 50, 53, 380
GsuI CTGGAG 2 cut(s) 29, 339
Hin1II CATG 2 cut(s) 145, 154
HinfI GANTC 1 cut(s) 75
HphI GGTGA 2 cut(s) 237, 431
Hpy166II GTNNAC 1 cut(s) 185
Hpy188III TCNNGA 3 cut(s) 110, 142, 278
Hpy8I GTNNAC 1 cut(s) 185
HpyAV CCTTC 1 cut(s) 396
HpyCH4III ACNGT 3 cut(s) 16, 86, 418
HpyCH4V TGCA 4 cut(s) 55, 60, 136, 185
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 434
HpyF3I CTNAG 1 cut(s) 68
Hsp92II CATG 2 cut(s) 145, 154
Kzo9I GATC 3 cut(s) 96, 144, 481
LguI GCTCTTC 1 cut(s) 111
LmnI GCTCC 1 cut(s) 358
LpnPI CCDG 6 cut(s) 15, 42, 154, 181, 363, 369
Lsp1109I GCAGC 4 cut(s) 32, 36, 39, 391
LweI GCATC 1 cut(s) 36
MaeI CTAG 1 cut(s) 110
MaeIII GTNAC 2 cut(s) 332, 412
MalI GATC 3 cut(s) 98, 146, 483
MboI GATC 3 cut(s) 96, 144, 481
MboII GAAGA 5 cut(s) 98, 125, 139, 205, 491
MhlI GDGCHC 2 cut(s) 84, 187
MluCI AATT 3 cut(s) 291, 320, 391
MmeI TCCRAC 1 cut(s) 250
MnlI CCTC 6 cut(s) 35, 149, 274, 277, 323, 427
MroXI GAANNNNTTC 1 cut(s) 291
MslI CAYNNNNRTG 1 cut(s) 447
MspR9I CCNGG 2 cut(s) 30, 169
MvaI CCWGG 2 cut(s) 30, 169
MwoI GCNNNNNNNGC 2 cut(s) 79, 434
NdeII GATC 3 cut(s) 96, 144, 481
NlaIII CATG 2 cut(s) 145, 154
NmeAIII GCCGAG 1 cut(s) 145
PagI TCATGA 1 cut(s) 141
PciSI GCTCTTC 1 cut(s) 111
PdmI GAANNNNTTC 1 cut(s) 291
PfeI GAWTC 1 cut(s) 75
PkrI GCNGC 4 cut(s) 22, 51, 54, 381
Psp6I CCWGG 2 cut(s) 28, 167
PspGI CCWGG 2 cut(s) 28, 167
PsrI GAACNNNNNNTAC 2 cut(s) 183, 215
PstNI CAGNNNCTG 1 cut(s) 29
RsaI GTAC 2 cut(s) 176, 224
RsaNI GTAC 2 cut(s) 175, 223
RseI CAYNNNNRTG 1 cut(s) 447
SapI GCTCTTC 1 cut(s) 111
SatI GCNGC 4 cut(s) 21, 50, 53, 380
Sau3AI GATC 3 cut(s) 96, 144, 481
ScrFI CCNGG 2 cut(s) 30, 169
SduI GDGCHC 2 cut(s) 84, 187
SfaNI GCATC 1 cut(s) 36
SmiMI CAYNNNNRTG 1 cut(s) 447
SmlI CTYRAG 3 cut(s) 278, 314, 400
SmoI CTYRAG 3 cut(s) 278, 314, 400
Sse9I AATT 3 cut(s) 291, 320, 391
SsiI CCGC 1 cut(s) 90
SspMI CTAG 1 cut(s) 110
StyD4I CCNGG 2 cut(s) 28, 167
TaaI ACNGT 3 cut(s) 16, 86, 418
TaqI TCGA 1 cut(s) 78
TasI AATT 3 cut(s) 291, 320, 391
TfiI GAWTC 1 cut(s) 75
TscAI CASTG 4 cut(s) 21, 30, 62, 91
TseI GCWGC 4 cut(s) 20, 49, 52, 379
TspDTI ATGAA 1 cut(s) 139
TspRI CASTG 4 cut(s) 21, 30, 62, 91
VneI GTGCAC 1 cut(s) 183
XbaI TCTAGA 1 cut(s) 109
XmnI GAANNNNTTC 1 cut(s) 291
XspI CTAG 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.