MD00G1148200.v1.1

Belongs to the alternative oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0006091
Physical Location & Seq
Reverse (-)
32488852 .. 32491834
2983 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1148200.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGATGATGGCGAGCCGGAGACACGCGGCGAACTCGATGGCGGGCACAGTACGGCTGTTCACCACTGCAACCGCTGCCAATAAGCCTCTGATGGCTGCTGCCGTTCATCTTCTTCCTCTGAATTATCTTCATAGCGGTTGTGGTGGTGCCCTAGTCCGGAGTGCTAGCACCAGTGCTGCAAATGTGGACGTGGCTGCAGAGGGTCATAACACTACGGCCCCTTCCGCTGCTGGCCACGGCGGTGGTAAGGATGATGAGAAGACGATCACCAGCTACTGGGGTGTGGGACCCACCAAAGTCACCAAGCAAGATGGAACCGAATGGAAGTGGACTTGCTTTAGGCCATGGGAGACGTACAAAGCGGACGTCTCGATAGATCTGAAGAAGCATCACGTGCCAACGACTTTGTTGGACAGAATGGCTTACCGGACCGTCAAATCTCTCCGATGGCCTACTGATCTATTCTTCCAGAGGCGGTATGGGTGCCGAGCAATGATGCTAGAAACAGTGGCAGCAGTTCCCGGAATGGTAGGAGGGATGCTGCTGCACTGCAAGTCCCTACGGCGGTTCGAGCACAGCGGAGGGTGGATCAAAGCTCTGCTGGAAGAAGCCGAGAACGAGCGAATGCACCTCATGACATTTATGGAAGTAGCTAACCCCAAGTGGTACGAGCGTGCCCTCGTCTTTGCGGTTCAAGGCGTCTTCTTCAACGCCTACTTCGTAGGGTATATGGTTTCCCCGAAATTCGCTCATCGAATGGTTGGGTACCTTGAGGAGGAAGCAATCCACTCGTACACTGAGTTCCTCAAGGAGCTGGATAAAGGGAACATAGAGAATGTGCCAGCTCCGGCGATTGCCATTGATTACTGGCAGCTGCCACCCGACTCGACTCTGAGAGATGTTGTTGCGGTTGTTAGGGCGGATGAGGCACACCACCGCGATGTCAATCACTTTGCATCGGATATACACTACAATGGACGGGAACTGAGGGAATCACCAGCTCCAATTGGTTATCACTGA

Protein Analysis

340

Amino Acids

37.91

Weight (kDa)

7.79

Isoelectric Point (pI)

39.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 129 - 320 1.8e-83 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 369
Acc65I GGTACC 1 cut(s) 765
AccB1I GGYRCC 3 cut(s) 146, 483, 765
AccB7I CCANNNNNTGG 1 cut(s) 276
AccII CGCG 2 cut(s) 26, 939
AccIII TCCGGA 1 cut(s) 156
AclWI GGATC 1 cut(s) 596
AcoI YGGCCR 1 cut(s) 232
AcsI RAATTY 1 cut(s) 743
AcuI CTGAAG 1 cut(s) 401
AcvI CACGTG 1 cut(s) 394
AcyI GRCGYC 2 cut(s) 366, 699
AfaI GTAC 5 cut(s) 51, 356, 668, 767, 794
AfiI CCNNNNNNNGG 4 cut(s) 156, 276, 564, 775
AgsI TTSAA 2 cut(s) 695, 709
AjiI CACGTC 1 cut(s) 190
AleI CACNNNNGTG 1 cut(s) 240
AluBI AGCT 7 cut(s) 273, 596, 653, 814, 845, 874, 1001
AluI AGCT 7 cut(s) 273, 596, 653, 814, 845, 874, 1001
Alw21I GWGCWC 1 cut(s) 576
Alw26I GTCTC 3 cut(s) 13, 344, 373
AlwI GGATC 1 cut(s) 596
AlwNI CAGNNNCTG 1 cut(s) 276
Aor13HI TCCGGA 1 cut(s) 156
AoxI GGCC 4 cut(s) 216, 232, 341, 449
ApoI RAATTY 1 cut(s) 743
ArsI GACNNNNNNTTYG 2 cut(s) 351, 383
Asp718I GGTACC 1 cut(s) 765
AspS9I GGNCC 3 cut(s) 217, 287, 429
AsuC2I CCSGG 1 cut(s) 522
AsuHPI GGTGA 4 cut(s) 52, 259, 292, 987
AsuNHI GCTAGC 1 cut(s) 164
AvaII GGWCC 2 cut(s) 287, 429
BaeGI GKGCMC 3 cut(s) 47, 151, 679
BaeI ACNNNNGTAYC 2 cut(s) 658, 691
BalI TGGCCA 1 cut(s) 234
BanI GGYRCC 3 cut(s) 146, 483, 765
BbrPI CACGTG 1 cut(s) 394
BbsI GAAGAC 2 cut(s) 266, 694
Bbv12I GWGCWC 1 cut(s) 576
BccI CCATC 4 cut(s) 31, 85, 305, 441
BceAI ACGGC 5 cut(s) 68, 86, 231, 253, 578
BcgI CGANNNNNNTGC 2 cut(s) 771, 805
BcnI CCSGG 1 cut(s) 522
BcoDI GTCTC 3 cut(s) 13, 344, 373
BfaI CTAG 3 cut(s) 152, 165, 500
BfmI CTRYAG 1 cut(s) 195
BglII AGATCT 1 cut(s) 376
Bme1390I CCNGG 1 cut(s) 522
Bme18I GGWCC 2 cut(s) 287, 429
BmgBI CACGTC 1 cut(s) 190
BmgT120I GGNCC 3 cut(s) 217, 287, 429
BmiI GGNNCC 7 cut(s) 148, 219, 288, 289, 316, 485, 767
BmrFI CCNGG 1 cut(s) 522
BmrI ACTGGG 1 cut(s) 286
BmsI GCATC 4 cut(s) 397, 486, 528, 965
BmtI GCTAGC 1 cut(s) 168
BmuI ACTGGG 1 cut(s) 286
BpiI GAAGAC 2 cut(s) 266, 694
BpuEI CTTGAG 2 cut(s) 791, 791
BpuMI CCSGG 1 cut(s) 522
BsaAI YACGTR 1 cut(s) 394
BsaBI GATNNNNATC 1 cut(s) 945
BsaHI GRCGYC 2 cut(s) 366, 699
BsaJI CCNNGG 2 cut(s) 235, 344
BsaWI WCCGGW 2 cut(s) 156, 426
Bsc4I CCNNNNNNNGG 4 cut(s) 156, 276, 564, 775
Bse1I ACTGG 3 cut(s) 171, 281, 872
Bse3DI GCAATG 1 cut(s) 498
Bse8I GATNNNNATC 1 cut(s) 945
BseAI TCCGGA 1 cut(s) 156
BseDI CCNNGG 2 cut(s) 235, 344
BseGI GGATG 3 cut(s) 256, 543, 928
BseJI GATNNNNATC 1 cut(s) 945
BseLI CCNNNNNNNGG 4 cut(s) 156, 276, 564, 775
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 3 cut(s) 789, 884, 977
BseNI ACTGG 3 cut(s) 171, 281, 872
BseRI GAGGAG 1 cut(s) 788
BseSI GKGCMC 3 cut(s) 47, 151, 679
BsgI GTGCAG 1 cut(s) 530
Bsh1236I CGCG 2 cut(s) 26, 939
BshFI GGCC 4 cut(s) 218, 234, 343, 451
BshNI GGYRCC 3 cut(s) 146, 483, 765
BsiHKAI GWGCWC 1 cut(s) 576
BsiSI CCGG 5 cut(s) 16, 157, 427, 522, 848
BslFI GGGAC 2 cut(s) 300, 541
BslI CCNNNNNNNGG 4 cut(s) 156, 276, 564, 775
BsmAI GTCTC 3 cut(s) 13, 344, 373
BsmBI CGTCTC 2 cut(s) 344, 373
BsmFI GGGAC 2 cut(s) 300, 541
BsmI GAATGC 1 cut(s) 630
BsnI GGCC 4 cut(s) 218, 234, 343, 451
Bsp1286I GDGCHC 4 cut(s) 47, 151, 576, 679
Bsp13I TCCGGA 1 cut(s) 156
Bsp143I GATC 4 cut(s) 264, 376, 457, 588
Bsp19I CCATGG 1 cut(s) 344
BspANI GGCC 4 cut(s) 218, 234, 343, 451
BspCNI CTCAG 3 cut(s) 790, 885, 978
BspEI TCCGGA 1 cut(s) 156
BspFNI CGCG 2 cut(s) 26, 939
BspHI TCATGA 1 cut(s) 633
BspLI GGNNCC 7 cut(s) 148, 219, 288, 289, 316, 485, 767
BspMAI CTGCAG 1 cut(s) 199
BspOI GCTAGC 1 cut(s) 168
BspPI GGATC 1 cut(s) 596
BspT107I GGYRCC 3 cut(s) 146, 483, 765
BsrDI GCAATG 1 cut(s) 498
BsrI ACTGG 3 cut(s) 171, 281, 872
BssECI CCNNGG 2 cut(s) 235, 344
BssMI GATC 4 cut(s) 264, 376, 457, 588
BssNI GRCGYC 2 cut(s) 366, 699
BssT1I CCWWGG 1 cut(s) 344
Bst4CI ACNGT 3 cut(s) 49, 433, 508
BstACI GRCGYC 2 cut(s) 366, 699
BstAPI GCANNNNNTGC 2 cut(s) 74, 394
BstBAI YACGTR 1 cut(s) 394
BstC8I GCNNGC 6 cut(s) 13, 43, 166, 232, 675, 843
BstDEI CTNAG 3 cut(s) 798, 893, 986
BstDSI CCRYGG 2 cut(s) 235, 344
BstENI CCTNNNNNAGG 1 cut(s) 773
BstF5I GGATG 3 cut(s) 256, 543, 928
BstFNI CGCG 2 cut(s) 26, 939
BstKTI GATC 4 cut(s) 267, 379, 460, 591
BstMAI GTCTC 3 cut(s) 13, 344, 373
BstMBI GATC 4 cut(s) 264, 376, 457, 588
BstMWI GCNNNNNNNGC 5 cut(s) 74, 224, 394, 571, 926
BstSCI CCNGG 1 cut(s) 520
BstSFI CTRYAG 1 cut(s) 195
BstSLI GKGCMC 3 cut(s) 47, 151, 679
BstUI CGCG 2 cut(s) 26, 939
BstV2I GAAGAC 2 cut(s) 266, 694
BstX2I RGATCY 1 cut(s) 376
BstXI CCANNNNNNTGG 1 cut(s) 242
BstYI RGATCY 1 cut(s) 376
BsuRI GGCC 4 cut(s) 218, 234, 343, 451
BtgI CCRYGG 2 cut(s) 235, 344
BtgZI GCGATG 1 cut(s) 954
BtrI CACGTC 1 cut(s) 190
BtsCI GGATG 3 cut(s) 256, 543, 928
BtsI GCAGTG 2 cut(s) 63, 547
BtsIMutI CAGTG 6 cut(s) 63, 178, 513, 547, 795, 1015
Cac8I GCNNGC 6 cut(s) 13, 43, 166, 232, 675, 843
CaiI CAGNNNCTG 1 cut(s) 276
CciI TCATGA 1 cut(s) 633
Cfr13I GGNCC 3 cut(s) 217, 287, 429
CpoI CGGWCCG 1 cut(s) 429
CseI GACGC 1 cut(s) 688
Csp6I GTAC 5 cut(s) 50, 355, 667, 766, 793
CspI CGGWCCG 1 cut(s) 429
CviAII CATG 2 cut(s) 345, 634
CviQI GTAC 5 cut(s) 50, 355, 667, 766, 793
DdeI CTNAG 3 cut(s) 798, 893, 986
DpnI GATC 4 cut(s) 266, 378, 459, 590
DpnII GATC 4 cut(s) 264, 376, 457, 588
EaeI YGGCCR 1 cut(s) 232
EciI GGCGGA 1 cut(s) 935
Eco130I CCWWGG 1 cut(s) 344
Eco47I GGWCC 2 cut(s) 287, 429
Eco57I CTGAAG 1 cut(s) 401
Eco72I CACGTG 1 cut(s) 394
EcoNI CCTNNNNNAGG 1 cut(s) 773
EcoO109I RGGNCCY 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 344
ErhI CCWWGG 1 cut(s) 344
Esp3I CGTCTC 2 cut(s) 344, 373
FaeI CATG 2 cut(s) 348, 637
FaqI GGGAC 2 cut(s) 300, 541
FatI CATG 2 cut(s) 344, 633
FauI CCCGC 1 cut(s) 34
FokI GGATG 3 cut(s) 263, 550, 935
FspBI CTAG 3 cut(s) 152, 165, 500
HaeIII GGCC 4 cut(s) 218, 234, 343, 451
HapII CCGG 5 cut(s) 16, 157, 427, 522, 848
HgaI GACGC 1 cut(s) 688
Hin1I GRCGYC 2 cut(s) 366, 699
Hin1II CATG 2 cut(s) 348, 637
HinfI GANTC 3 cut(s) 884, 889, 992
HpaII CCGG 5 cut(s) 16, 157, 427, 522, 848
HphI GGTGA 4 cut(s) 52, 259, 292, 987
Hpy166II GTNNAC 4 cut(s) 60, 187, 330, 795
Hpy188I TCNGA 6 cut(s) 90, 120, 381, 446, 894, 961
Hpy188III TCNNGA 4 cut(s) 157, 370, 469, 634
Hpy8I GTNNAC 4 cut(s) 60, 187, 330, 795
HpyAV CCTTC 1 cut(s) 231
HpyCH4III ACNGT 3 cut(s) 49, 433, 508
HpyCH4IV ACGT 4 cut(s) 189, 353, 366, 393
HpyCH4V TGCA 7 cut(s) 68, 179, 197, 547, 552, 628, 956
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 224, 394, 571, 926
HpyF3I CTNAG 3 cut(s) 798, 893, 986
HpySE526I ACGT 4 cut(s) 189, 353, 366, 393
Hsp92I GRCGYC 2 cut(s) 366, 699
Hsp92II CATG 2 cut(s) 348, 637
KflI GGGWCCC 1 cut(s) 287
Kpn2I TCCGGA 1 cut(s) 156
KpnI GGTACC 1 cut(s) 769
Kzo9I GATC 4 cut(s) 264, 376, 457, 588
LmnI GCTCC 3 cut(s) 811, 850, 1006
LweI GCATC 4 cut(s) 397, 486, 528, 965
MaeI CTAG 3 cut(s) 152, 165, 500
MaeII ACGT 4 cut(s) 189, 353, 366, 393
MaeIII GTNAC 1 cut(s) 298
MalI GATC 4 cut(s) 266, 378, 459, 590
MboI GATC 4 cut(s) 264, 376, 457, 588
MboII GAAGA 9 cut(s) 101, 104, 119, 271, 394, 457, 617, 694, 697
MfeI CAATTG 1 cut(s) 1005
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 4 cut(s) 47, 151, 576, 679
MlsI TGGCCA 1 cut(s) 234
MluCI AATT 3 cut(s) 121, 743, 1005
MluNI TGGCCA 1 cut(s) 234
MlyI GAGTC 2 cut(s) 878, 883
MmeI TCCRAC 1 cut(s) 390
Mox20I TGGCCA 1 cut(s) 234
MroI TCCGGA 1 cut(s) 156
MscI TGGCCA 1 cut(s) 234
MslI CAYNNNNRTG 3 cut(s) 240, 939, 972
Msp20I TGGCCA 1 cut(s) 234
MspA1I CMGCKG 4 cut(s) 74, 227, 579, 874
MspI CCGG 5 cut(s) 16, 157, 427, 522, 848
MspR9I CCNGG 1 cut(s) 522
MunI CAATTG 1 cut(s) 1005
Mva1269I GAATGC 1 cut(s) 630
MvnI CGCG 2 cut(s) 26, 939
MwoI GCNNNNNNNGC 5 cut(s) 74, 224, 394, 571, 926
NciI CCSGG 1 cut(s) 522
NcoI CCATGG 1 cut(s) 344
NdeII GATC 4 cut(s) 264, 376, 457, 588
NheI GCTAGC 1 cut(s) 164
NlaIII CATG 2 cut(s) 348, 637
NlaIV GGNNCC 7 cut(s) 148, 219, 288, 289, 316, 485, 767
NmeAIII GCCGAG 2 cut(s) 512, 637
NmuCI GTSAC 1 cut(s) 298
OliI CACNNNNGTG 1 cut(s) 240
PagI TCATGA 1 cut(s) 633
PcsI WCGNNNNNNNCGW 1 cut(s) 717
PctI GAATGC 1 cut(s) 630
PfeI GAWTC 1 cut(s) 992
PflMI CCANNNNNTGG 1 cut(s) 276
PfoI TCCNGGA 1 cut(s) 520
PleI GAGTC 2 cut(s) 878, 883
PmaCI CACGTG 1 cut(s) 394
PmlI CACGTG 1 cut(s) 394
PpsI GAGTC 2 cut(s) 878, 883
Ppu21I YACGTR 1 cut(s) 394
PpuMI RGGWCCY 1 cut(s) 287
Psp5II RGGWCCY 1 cut(s) 287
PspCI CACGTG 1 cut(s) 394
PspN4I GGNNCC 7 cut(s) 148, 219, 288, 289, 316, 485, 767
PspPI GGNCC 3 cut(s) 217, 287, 429
PspPPI RGGWCCY 1 cut(s) 287
PsrI GAACNNNNNNTAC 2 cut(s) 785, 817
PstI CTGCAG 1 cut(s) 199
PstNI CAGNNNCTG 1 cut(s) 276
PsuI RGATCY 1 cut(s) 376
PvuII CAGCTG 1 cut(s) 874
RsaI GTAC 5 cut(s) 51, 356, 668, 767, 794
RsaNI GTAC 5 cut(s) 50, 355, 667, 766, 793
RseI CAYNNNNRTG 3 cut(s) 240, 939, 972
Rsr2I CGGWCCG 1 cut(s) 429
RsrII CGGWCCG 1 cut(s) 429
Sau3AI GATC 4 cut(s) 264, 376, 457, 588
Sau96I GGNCC 3 cut(s) 217, 287, 429
SchI GAGTC 2 cut(s) 878, 883
ScrFI CCNGG 1 cut(s) 522
SduI GDGCHC 4 cut(s) 47, 151, 576, 679
SfaNI GCATC 4 cut(s) 397, 486, 528, 965
SfcI CTRYAG 1 cut(s) 195
SinI GGWCC 2 cut(s) 287, 429
SmiMI CAYNNNNRTG 3 cut(s) 240, 939, 972
SmlI CTYRAG 2 cut(s) 770, 806
SmoI CTYRAG 2 cut(s) 770, 806
Sse9I AATT 3 cut(s) 121, 743, 1005
SspMI CTAG 3 cut(s) 152, 165, 500
StyD4I CCNGG 1 cut(s) 520
StyI CCWWGG 1 cut(s) 344
TaaI ACNGT 3 cut(s) 49, 433, 508
TaiI ACGT 4 cut(s) 192, 356, 369, 396
TaqI TCGA 5 cut(s) 35, 371, 570, 754, 887
TasI AATT 3 cut(s) 121, 743, 1005
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 1 cut(s) 992
TscAI CASTG 5 cut(s) 70, 178, 513, 554, 802
TseFI GTSAC 1 cut(s) 298
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 2 cut(s) 95, 119
TspRI CASTG 5 cut(s) 70, 178, 513, 554, 802
Van91I CCANNNNNTGG 1 cut(s) 276
VpaK11BI GGWCC 2 cut(s) 287, 429
XagI CCTNNNNNAGG 1 cut(s) 773
XapI RAATTY 1 cut(s) 743
XcmI CCANNNNNNNNNTGG 1 cut(s) 476
XspI CTAG 3 cut(s) 152, 165, 500
ZraI GACGTC 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.