RchiOBHm_Chr7g0213861

Belongs to the alternative oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0006091
Physical Location & Seq
Reverse (-)
30971655 .. 30972691
1037 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19138

Sequence Viewer

Length: 681 bp
ATGGTACTGAATGGAAATGGAATTGCTTCAGAGTATCCATGGGAGACGTACAAGGCCGACACCACGATAGATCTCCAGAAGCATCACGTGCCTACAACTTTGATCGACAAAATGGCGTACTGGACGGTCAAGTCTCTCCGCTGGCCTACCGACCTATTCTTCCAGACAGTGGCAGCAGTGCCTGGAATGGTAGGAGGGATGCTGCTGCACTGCAAATTGCTTAGGCGATTTGAGCACAGTGGCGGATGGATCAAAGCTCTTCTAGAAGAAGCCGAGAACGAGCGTATGCACCTCATGACCTTCATGGAAGTCGCCAAGCCCTGGTGGTATGAGCGCGCGCTTGTATTTGCCGTTCAAGGTGTTTTCTTCAATGCGTACTTCTTGGGATATATGATTTCACCCAAGTTTGCTCATAGAATGGTTGGATATCTTGAGGAGGAAGCAATTCACTCATATACCGAGTTCCTCAAGGAATTAGACAAAGGTAACATTGAGAATGTTCCAGCTCCAGCTATTGCTATTGATTATTGGCAGCTACCTTCCAATTCCACCTTGAGAGATGTTGTTACAGTTGTTAGGGCAGACGAGGCTCACCACCGAGATGTCAATCACTTCGCTTCGGACATACATTATAATGGACGTGAACTGAAGGAATCTCCAGCTCCAATTGGTTACCATTGA

Protein Analysis

226

Amino Acids

26.07

Weight (kDa)

5.93

Isoelectric Point (pI)

40.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 52 - 207 1.1e-72 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 633
AasI GACNNNNNNGTC 1 cut(s) 130
AccB7I CCANNNNNTGG 2 cut(s) 169, 321
AccII CGCG 2 cut(s) 336, 338
AciI CCGC 2 cut(s) 139, 243
AclWI GGATC 1 cut(s) 257
AcuI CTGAAG 2 cut(s) 12, 668
AcvI CACGTG 1 cut(s) 88
AfaI GTAC 4 cut(s) 6, 50, 119, 377
AfiI CCNNNNNNNGG 2 cut(s) 169, 321
AgsI TTSAA 2 cut(s) 356, 370
AjiI CACGTC 1 cut(s) 641
AjnI CCWGG 2 cut(s) 181, 320
AluBI AGCT 5 cut(s) 257, 506, 512, 535, 662
AluI AGCT 5 cut(s) 257, 506, 512, 535, 662
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 2 cut(s) 38, 138
AlwI GGATC 1 cut(s) 257
AlwNI CAGNNNCTG 1 cut(s) 182
AoxI GGCC 2 cut(s) 54, 143
ApeKI GCWGC 4 cut(s) 173, 202, 205, 532
Asp700I GAANNNNTTC 2 cut(s) 25, 444
AspLEI GCGC 3 cut(s) 336, 338, 340
AsuHPI GGTGA 2 cut(s) 390, 584
BbrPI CACGTG 1 cut(s) 88
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 4 cut(s) 185, 189, 192, 544
BccI CCATC 1 cut(s) 240
BceAI ACGGC 1 cut(s) 335
BciT130I CCWGG 2 cut(s) 183, 322
BciVI GTATCC 1 cut(s) 45
BcoDI GTCTC 2 cut(s) 38, 138
BfaI CTAG 1 cut(s) 263
BfuI GTATCC 1 cut(s) 45
BglII AGATCT 1 cut(s) 70
BisI GCNGC 4 cut(s) 174, 203, 206, 533
BlsI GCNGC 4 cut(s) 175, 204, 207, 534
Bme1390I CCNGG 2 cut(s) 183, 322
BmgBI CACGTC 1 cut(s) 641
BmrFI CCNGG 2 cut(s) 183, 322
BmsI GCATC 2 cut(s) 91, 189
BpmI CTGGAG 3 cut(s) 59, 492, 642
Bpu10I CCTNAGC 1 cut(s) 221
BpuEI CTTGAG 3 cut(s) 452, 452, 574
BsaAI YACGTR 1 cut(s) 88
BsaBI GATNNNNATC 1 cut(s) 606
BsaJI CCNNGG 2 cut(s) 38, 320
Bsc4I CCNNNNNNNGG 2 cut(s) 169, 321
Bse1I ACTGG 1 cut(s) 125
Bse8I GATNNNNATC 1 cut(s) 606
BseBI CCWGG 2 cut(s) 183, 322
BseDI CCNNGG 2 cut(s) 38, 320
BseGI GGATG 2 cut(s) 204, 251
BseJI GATNNNNATC 1 cut(s) 606
BseLI CCNNNNNNNGG 2 cut(s) 169, 321
BseNI ACTGG 1 cut(s) 125
BsePI GCGCGC 2 cut(s) 334, 336
BseRI GAGGAG 1 cut(s) 449
BseXI GCAGC 4 cut(s) 185, 189, 192, 544
BsgI GTGCAG 1 cut(s) 191
Bsh1236I CGCG 2 cut(s) 336, 338
BshFI GGCC 2 cut(s) 56, 145
BsiHKAI GWGCWC 1 cut(s) 237
BslI CCNNNNNNNGG 2 cut(s) 169, 321
BsmAI GTCTC 2 cut(s) 38, 138
BsmBI CGTCTC 1 cut(s) 38
BsnI GGCC 2 cut(s) 56, 145
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 3 cut(s) 70, 102, 249
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 2 cut(s) 139, 243
BspANI GGCC 2 cut(s) 56, 145
BspFNI CGCG 2 cut(s) 336, 338
BspHI TCATGA 1 cut(s) 294
BspPI GGATC 1 cut(s) 257
BspQI GCTCTTC 1 cut(s) 264
BsrI ACTGG 1 cut(s) 125
BssECI CCNNGG 2 cut(s) 38, 320
BssHII GCGCGC 2 cut(s) 334, 336
BssMI GATC 3 cut(s) 70, 102, 249
BssT1I CCWWGG 1 cut(s) 38
Bst2UI CCWGG 2 cut(s) 183, 322
Bst4CI ACNGT 4 cut(s) 127, 169, 239, 571
Bst6I CTCTTC 1 cut(s) 264
BstAPI GCANNNNNTGC 1 cut(s) 88
BstBAI YACGTR 1 cut(s) 88
BstC8I GCNNGC 3 cut(s) 143, 336, 338
BstDEI CTNAG 1 cut(s) 221
BstDSI CCRYGG 1 cut(s) 38
BstEII GGTNACC 1 cut(s) 671
BstF5I GGATG 2 cut(s) 204, 251
BstFNI CGCG 2 cut(s) 336, 338
BstHHI GCGC 3 cut(s) 336, 338, 340
BstKTI GATC 3 cut(s) 73, 105, 252
BstMAI GTCTC 2 cut(s) 38, 138
BstMBI GATC 3 cut(s) 70, 102, 249
BstMWI GCNNNNNNNGC 3 cut(s) 88, 232, 587
BstNI CCWGG 2 cut(s) 183, 322
BstPI GGTNACC 1 cut(s) 671
BstSCI CCNGG 2 cut(s) 181, 320
BstUI CGCG 2 cut(s) 336, 338
BstV1I GCAGC 4 cut(s) 185, 189, 192, 544
BstX2I RGATCY 1 cut(s) 70
BstYI RGATCY 1 cut(s) 70
BsuI GTATCC 1 cut(s) 45
BsuRI GGCC 2 cut(s) 56, 145
BtgI CCRYGG 1 cut(s) 38
BtrI CACGTC 1 cut(s) 641
BtsCI GGATG 2 cut(s) 204, 251
BtsI GCAGTG 2 cut(s) 183, 208
BtsIMutI CAGTG 4 cut(s) 174, 183, 208, 244
Cac8I GCNNGC 3 cut(s) 143, 336, 338
CaiI CAGNNNCTG 1 cut(s) 182
CciI TCATGA 1 cut(s) 294
CfoI GCGC 3 cut(s) 336, 338, 340
Csp6I GTAC 4 cut(s) 5, 49, 118, 376
CviAII CATG 3 cut(s) 39, 295, 304
CviQI GTAC 4 cut(s) 5, 49, 118, 376
DdeI CTNAG 1 cut(s) 221
DpnI GATC 3 cut(s) 72, 104, 251
DpnII GATC 3 cut(s) 70, 102, 249
DrdI GACNNNNNNGTC 1 cut(s) 130
DseDI GACNNNNNNGTC 1 cut(s) 130
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
EciI GGCGGA 1 cut(s) 258
Eco130I CCWWGG 1 cut(s) 38
Eco32I GATATC 1 cut(s) 428
Eco57I CTGAAG 2 cut(s) 12, 668
Eco72I CACGTG 1 cut(s) 88
Eco91I GGTNACC 1 cut(s) 671
EcoO65I GGTNACC 1 cut(s) 671
EcoRII CCWGG 2 cut(s) 181, 320
EcoRV GATATC 1 cut(s) 428
EcoT14I CCWWGG 1 cut(s) 38
ErhI CCWWGG 1 cut(s) 38
Esp3I CGTCTC 1 cut(s) 38
FaeI CATG 3 cut(s) 42, 298, 307
FatI CATG 3 cut(s) 38, 294, 303
Fnu4HI GCNGC 4 cut(s) 174, 203, 206, 533
FokI GGATG 2 cut(s) 211, 258
Fsp4HI GCNGC 4 cut(s) 174, 203, 206, 533
FspBI CTAG 1 cut(s) 263
GlaI GCGC 3 cut(s) 335, 337, 339
GluI GCNGC 4 cut(s) 174, 203, 206, 533
GsuI CTGGAG 3 cut(s) 59, 492, 642
HaeIII GGCC 2 cut(s) 56, 145
HhaI GCGC 3 cut(s) 336, 338, 340
Hin1II CATG 3 cut(s) 42, 298, 307
Hin6I GCGC 3 cut(s) 334, 336, 338
HinP1I GCGC 3 cut(s) 334, 336, 338
HinfI GANTC 1 cut(s) 653
HphI GGTGA 2 cut(s) 390, 584
Hpy166II GTNNAC 1 cut(s) 644
Hpy188I TCNGA 2 cut(s) 31, 622
Hpy188III TCNNGA 5 cut(s) 76, 163, 263, 295, 431
Hpy8I GTNNAC 1 cut(s) 644
HpyAV CCTTC 3 cut(s) 310, 549, 643
HpyCH4III ACNGT 4 cut(s) 127, 169, 239, 571
HpyCH4IV ACGT 3 cut(s) 47, 87, 640
HpyCH4V TGCA 3 cut(s) 208, 213, 289
HpyF10VI GCNNNNNNNGC 3 cut(s) 88, 232, 587
HpyF3I CTNAG 1 cut(s) 221
HpySE526I ACGT 3 cut(s) 47, 87, 640
Hsp92II CATG 3 cut(s) 42, 298, 307
HspAI GCGC 3 cut(s) 334, 336, 338
Kzo9I GATC 3 cut(s) 70, 102, 249
LguI GCTCTTC 1 cut(s) 264
LmnI GCTCC 2 cut(s) 511, 667
Lsp1109I GCAGC 4 cut(s) 185, 189, 192, 544
LweI GCATC 2 cut(s) 91, 189
MaeI CTAG 1 cut(s) 263
MaeII ACGT 3 cut(s) 47, 87, 640
MaeIII GTNAC 3 cut(s) 485, 565, 671
MalI GATC 3 cut(s) 72, 104, 251
MboI GATC 3 cut(s) 70, 102, 249
MboII GAAGA 4 cut(s) 151, 251, 278, 358
MfeI CAATTG 1 cut(s) 666
MflI RGATCY 1 cut(s) 70
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 6 cut(s) 21, 215, 444, 473, 544, 666
MmeI TCCRAC 1 cut(s) 403
MnlI CCTC 6 cut(s) 188, 302, 427, 430, 476, 580
MroXI GAANNNNTTC 2 cut(s) 25, 444
MslI CAYNNNNRTG 2 cut(s) 600, 633
MspA1I CMGCKG 1 cut(s) 141
MspR9I CCNGG 2 cut(s) 183, 322
MunI CAATTG 1 cut(s) 666
MvaI CCWGG 2 cut(s) 183, 322
MvnI CGCG 2 cut(s) 336, 338
MwoI GCNNNNNNNGC 3 cut(s) 88, 232, 587
NcoI CCATGG 1 cut(s) 38
NdeII GATC 3 cut(s) 70, 102, 249
NlaIII CATG 3 cut(s) 42, 298, 307
NmeAIII GCCGAG 1 cut(s) 298
PagI TCATGA 1 cut(s) 294
PauI GCGCGC 2 cut(s) 334, 336
PciSI GCTCTTC 1 cut(s) 264
PdmI GAANNNNTTC 2 cut(s) 25, 444
PfeI GAWTC 1 cut(s) 653
PflMI CCANNNNNTGG 2 cut(s) 169, 321
PkrI GCNGC 4 cut(s) 175, 204, 207, 534
PmaCI CACGTG 1 cut(s) 88
PmlI CACGTG 1 cut(s) 88
Ppu21I YACGTR 1 cut(s) 88
PsiI TTATAA 1 cut(s) 633
Psp6I CCWGG 2 cut(s) 181, 320
PspCI CACGTG 1 cut(s) 88
PspEI GGTNACC 1 cut(s) 671
PspGI CCWGG 2 cut(s) 181, 320
PsrI GAACNNNNNNTAC 2 cut(s) 336, 368
PstNI CAGNNNCTG 1 cut(s) 182
PsuI RGATCY 1 cut(s) 70
PteI GCGCGC 2 cut(s) 334, 336
RsaI GTAC 4 cut(s) 6, 50, 119, 377
RsaNI GTAC 4 cut(s) 5, 49, 118, 376
RseI CAYNNNNRTG 2 cut(s) 600, 633
SapI GCTCTTC 1 cut(s) 264
SatI GCNGC 4 cut(s) 174, 203, 206, 533
Sau3AI GATC 3 cut(s) 70, 102, 249
ScrFI CCNGG 2 cut(s) 183, 322
SduI GDGCHC 1 cut(s) 237
SfaNI GCATC 2 cut(s) 91, 189
SmiMI CAYNNNNRTG 2 cut(s) 600, 633
SmlI CTYRAG 3 cut(s) 431, 467, 553
SmoI CTYRAG 3 cut(s) 431, 467, 553
Sse9I AATT 6 cut(s) 21, 215, 444, 473, 544, 666
SsiI CCGC 2 cut(s) 139, 243
SspMI CTAG 1 cut(s) 263
StyD4I CCNGG 2 cut(s) 181, 320
StyI CCWWGG 1 cut(s) 38
TaaI ACNGT 4 cut(s) 127, 169, 239, 571
TaiI ACGT 3 cut(s) 50, 90, 643
TaqI TCGA 1 cut(s) 105
TasI AATT 6 cut(s) 21, 215, 444, 473, 544, 666
TfiI GAWTC 1 cut(s) 653
TscAI CASTG 4 cut(s) 174, 183, 215, 244
TseI GCWGC 4 cut(s) 173, 202, 205, 532
TspDTI ATGAA 1 cut(s) 292
TspRI CASTG 4 cut(s) 174, 183, 215, 244
Van91I CCANNNNNTGG 2 cut(s) 169, 321
XbaI TCTAGA 1 cut(s) 262
XmnI GAANNNNTTC 2 cut(s) 25, 444
XspI CTAG 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.