AT3G22370

Belongs to the alternative oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006091
Physical Location & Seq
Forward (+)
7906521 .. 7908746
2226 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22370.1

Sequence Viewer

Length: 1065 bp
ATGATGATAACTCGCGGTGGAGCCAAGGCGGCGAAATCGCTGTTAGTGGCGGCTGGACCACGTTTGTTCTCGACGGTCCGTACGGTTTCGTCTCACGAGGCTTTATCAGCAAGCCATATTTTGAAGCCTGGTGTTACATCTGCTTGGATATGGACTAGAGCTCCGACGATTGGAGGTATGAGATTCGCTAGCACGATCACTCTGGGAGAGAAAACTCCGATGAAGGAGGAGGACGCGAATCAGAAGAAAACAGAGAACGAATCCACCGGTGGAGACGCCGCCGGAGGTAATAACAAGGGAGATAAAGGAATCGCGAGCTATTGGGGTGTTGAACCTAATAAGATTACTAAAGAAGATGGTTCTGAATGGAAGTGGAACTGTTTCAGGCCATGGGAAACGTATAAAGCTGATATAACGATAGATCTGAAGAAGCATCATGTTCCAACGACGTTTCTTGATAGAATAGCTTATTGGACTGTTAAATCTCTTCGTTGGCCTACCGATTTGTTCTTCCAGAGGAGATATGGATGTCGAGCTATGATGCTTGAAACTGTAGCAGCAGTACCTGGAATGGTTGGAGGAATGTTACTACACTGCAAATCGCTTCGACGTTTTGAGCAAAGTGGAGGATGGATTAAGGCTCTTCTTGAGGAAGCAGAGAATGAGAGAATGCATCTTATGACATTCATGGAAGTCGCGAAACCGAAATGGTACGAGAGAGCGCTCGTGATCACTGTGCAAGGAGTCTTCTTCAACGCTTATTTCCTTGGTTACTTAATCTCTCCCAAGTTTGCTCATCGTATGGTTGGGTACCTTGAAGAAGAAGCGATCCATTCTTATACTGAGTTTCTCAAGGAACTTGACAAAGGTAACATTGAGAATGTTCCTGCTCCGGCTATTGCTATTGATTACTGGAGGCTTCCTGCTGATGCGACACTTCGTGATGTTGTGATGGTTGTTCGTGCTGACGAGGCTCATCACCGTGATGTAAACCATTTTGCATCTGATATTCACTACCAAGGTCGTGAACTAAAGGAAGCTCCAGCTCCAATTGGGTATCATTGA

Protein Analysis

354

Amino Acids

39.98

Weight (kDa)

8.56

Isoelectric Point (pI)

35.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 144 - 335 1.6e-84 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 810
AccB1I GGYRCC 1 cut(s) 810
AccII CGCG 4 cut(s) 15, 236, 314, 698
AciI CCGC 4 cut(s) 15, 29, 50, 279
AclWI GGATC 1 cut(s) 823
AcuI CTGAAG 1 cut(s) 446
AcyI GRCGYC 1 cut(s) 276
AdeI CACNNNGTG 1 cut(s) 941
AfaI GTAC 4 cut(s) 82, 564, 713, 812
AfeI AGCGCT 1 cut(s) 723
AfiI CCNNNNNNNGG 1 cut(s) 170
AgeI ACCGGT 1 cut(s) 266
AgsI TTSAA 5 cut(s) 124, 332, 548, 754, 818
AjnI CCWGG 2 cut(s) 127, 565
AjuI GAANNNNNNNTTGG 2 cut(s) 454, 486
AluBI AGCT 7 cut(s) 161, 318, 407, 467, 536, 1040, 1046
AluI AGCT 7 cut(s) 161, 318, 407, 467, 536, 1040, 1046
Alw21I GWGCWC 1 cut(s) 163
Alw26I GTCTC 2 cut(s) 96, 267
AlwI GGATC 1 cut(s) 823
AlwNI CAGNNNCTG 1 cut(s) 566
Aor51HI AGCGCT 1 cut(s) 723
AoxI GGCC 2 cut(s) 386, 494
ApeKI GCWGC 1 cut(s) 557
AsiGI ACCGGT 1 cut(s) 266
Asp700I GAANNNNTTC 1 cut(s) 380
Asp718I GGTACC 1 cut(s) 810
AspLEI GCGC 1 cut(s) 724
AspS9I GGNCC 2 cut(s) 56, 76
AsuHPI GGTGA 1 cut(s) 971
AsuNHI GCTAGC 1 cut(s) 188
AvaII GGWCC 2 cut(s) 56, 76
BanI GGYRCC 1 cut(s) 810
BanII GRGCYC 1 cut(s) 163
BauI CACGAG 2 cut(s) 95, 725
BbsI GAAGAC 1 cut(s) 739
Bbv12I GWGCWC 1 cut(s) 163
BbvI GCAGC 1 cut(s) 569
BccI CCATC 3 cut(s) 350, 624, 946
BciT130I CCWGG 2 cut(s) 129, 567
BclI TGATCA 1 cut(s) 729
BcoDI GTCTC 2 cut(s) 96, 267
BfaI CTAG 2 cut(s) 156, 189
BfmI CTRYAG 1 cut(s) 552
BfoI RGCGCY 1 cut(s) 725
BglI GCCNNNNNGGC 1 cut(s) 29
BglII AGATCT 1 cut(s) 421
BisI GCNGC 4 cut(s) 30, 51, 279, 558
BlsI GCNGC 4 cut(s) 31, 52, 280, 559
Bme1390I CCNGG 2 cut(s) 129, 567
Bme18I GGWCC 2 cut(s) 56, 76
BmgT120I GGNCC 2 cut(s) 56, 76
BmiI GGNNCC 2 cut(s) 22, 812
BmrFI CCNGG 2 cut(s) 129, 567
BmsI GCATC 5 cut(s) 442, 531, 682, 919, 1010
BmtI GCTAGC 1 cut(s) 192
BpiI GAAGAC 1 cut(s) 739
BpmI CTGGAG 2 cut(s) 934, 1026
BpuEI CTTGAG 2 cut(s) 668, 836
BsaHI GRCGYC 1 cut(s) 276
BsaJI CCNNGG 4 cut(s) 24, 389, 766, 1018
BsaWI WCCGGW 1 cut(s) 266
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 1 cut(s) 917
BseBI CCWGG 2 cut(s) 129, 567
BseDI CCNNGG 4 cut(s) 24, 389, 766, 1018
BseGI GGATG 2 cut(s) 533, 635
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMII CTCAG 1 cut(s) 834
BseNI ACTGG 1 cut(s) 917
BseRI GAGGAG 2 cut(s) 242, 532
BseXI GCAGC 1 cut(s) 569
Bsh1236I CGCG 4 cut(s) 15, 236, 314, 698
BshFI GGCC 2 cut(s) 388, 496
BshNI GGYRCC 1 cut(s) 810
BshTI ACCGGT 1 cut(s) 266
BsiHKAI GWGCWC 1 cut(s) 163
BsiSI CCGG 3 cut(s) 267, 282, 893
BsiWI CGTACG 1 cut(s) 80
BslI CCNNNNNNNGG 1 cut(s) 170
BsmAI GTCTC 2 cut(s) 96, 267
BsmBI CGTCTC 2 cut(s) 96, 267
BsmI GAATGC 1 cut(s) 675
BsnI GGCC 2 cut(s) 388, 496
Bsp1286I GDGCHC 1 cut(s) 163
Bsp143I GATC 4 cut(s) 195, 421, 729, 828
Bsp19I CCATGG 1 cut(s) 389
Bsp68I TCGCGA 2 cut(s) 314, 698
BspACI CCGC 4 cut(s) 15, 29, 50, 279
BspANI GGCC 2 cut(s) 388, 496
BspCNI CTCAG 1 cut(s) 835
BspFNI CGCG 4 cut(s) 15, 236, 314, 698
BspLI GGNNCC 2 cut(s) 22, 812
BspOI GCTAGC 1 cut(s) 192
BspPI GGATC 1 cut(s) 823
BspQI GCTCTTC 1 cut(s) 648
BspT107I GGYRCC 1 cut(s) 810
BsrFI RCCGGY 1 cut(s) 266
BsrI ACTGG 1 cut(s) 917
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 4 cut(s) 24, 389, 766, 1018
BssMI GATC 4 cut(s) 195, 421, 729, 828
BssNI GRCGYC 1 cut(s) 276
BssSI CACGAG 2 cut(s) 95, 725
BssT1I CCWWGG 4 cut(s) 24, 389, 766, 1018
Bst2BI CACGAG 2 cut(s) 95, 725
Bst2UI CCWGG 2 cut(s) 129, 567
Bst4CI ACNGT 7 cut(s) 76, 85, 380, 478, 553, 736, 983
Bst6I CTCTTC 2 cut(s) 492, 648
BstACI GRCGYC 1 cut(s) 276
BstC8I GCNNGC 3 cut(s) 112, 190, 316
BstDEI CTNAG 1 cut(s) 843
BstDSI CCRYGG 1 cut(s) 389
BstF5I GGATG 2 cut(s) 533, 635
BstFNI CGCG 4 cut(s) 15, 236, 314, 698
BstH2I RGCGCY 1 cut(s) 725
BstHHI GCGC 1 cut(s) 724
BstKTI GATC 4 cut(s) 198, 424, 732, 831
BstMAI GTCTC 2 cut(s) 96, 267
BstMBI GATC 4 cut(s) 195, 421, 729, 828
BstMWI GCNNNNNNNGC 3 cut(s) 29, 107, 971
BstNI CCWGG 2 cut(s) 129, 567
BstSCI CCNGG 2 cut(s) 127, 565
BstSFI CTRYAG 1 cut(s) 552
BstUI CGCG 4 cut(s) 15, 236, 314, 698
BstV1I GCAGC 1 cut(s) 569
BstV2I GAAGAC 1 cut(s) 739
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
BsuRI GGCC 2 cut(s) 388, 496
BtgI CCRYGG 1 cut(s) 389
BtsCI GGATG 2 cut(s) 533, 635
BtsI GCAGTG 1 cut(s) 592
BtsIMutI CAGTG 2 cut(s) 592, 732
BtuMI TCGCGA 2 cut(s) 314, 698
Cac8I GCNNGC 3 cut(s) 112, 190, 316
CaiI CAGNNNCTG 1 cut(s) 566
CfoI GCGC 1 cut(s) 724
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 2 cut(s) 56, 76
CpoI CGGWCCG 1 cut(s) 76
CseI GACGC 2 cut(s) 242, 284
Csp6I GTAC 4 cut(s) 81, 563, 712, 811
CspAI ACCGGT 1 cut(s) 266
CspI CGGWCCG 1 cut(s) 76
CviAII CATG 3 cut(s) 390, 437, 688
CviQI GTAC 4 cut(s) 81, 563, 712, 811
DdeI CTNAG 1 cut(s) 843
DpnI GATC 4 cut(s) 197, 423, 731, 830
DpnII GATC 4 cut(s) 195, 421, 729, 828
DraIII CACNNNGTG 1 cut(s) 941
Eam1104I CTCTTC 2 cut(s) 492, 648
EarI CTCTTC 2 cut(s) 492, 648
Ecl136II GAGCTC 1 cut(s) 161
Eco130I CCWWGG 4 cut(s) 24, 389, 766, 1018
Eco24I GRGCYC 1 cut(s) 163
Eco47I GGWCC 2 cut(s) 56, 76
Eco47III AGCGCT 1 cut(s) 723
Eco53kI GAGCTC 1 cut(s) 161
Eco57I CTGAAG 1 cut(s) 446
EcoICRI GAGCTC 1 cut(s) 161
EcoRII CCWGG 2 cut(s) 127, 565
EcoT14I CCWWGG 4 cut(s) 24, 389, 766, 1018
EcoT22I ATGCAT 1 cut(s) 675
EcoT38I GRGCYC 1 cut(s) 163
ErhI CCWWGG 4 cut(s) 24, 389, 766, 1018
Esp3I CGTCTC 2 cut(s) 96, 267
FaeI CATG 3 cut(s) 393, 440, 691
FatI CATG 3 cut(s) 389, 436, 687
FbaI TGATCA 1 cut(s) 729
Fnu4HI GCNGC 4 cut(s) 30, 51, 279, 558
FokI GGATG 2 cut(s) 540, 642
FriOI GRGCYC 1 cut(s) 163
Fsp4HI GCNGC 4 cut(s) 30, 51, 279, 558
FspBI CTAG 2 cut(s) 156, 189
GlaI GCGC 1 cut(s) 723
GluI GCNGC 4 cut(s) 30, 51, 279, 558
GsuI CTGGAG 2 cut(s) 934, 1026
HaeII RGCGCY 1 cut(s) 725
HaeIII GGCC 2 cut(s) 388, 496
HapII CCGG 3 cut(s) 267, 282, 893
HgaI GACGC 2 cut(s) 242, 284
HhaI GCGC 1 cut(s) 724
Hin1I GRCGYC 1 cut(s) 276
Hin1II CATG 3 cut(s) 393, 440, 691
Hin6I GCGC 1 cut(s) 722
HinP1I GCGC 1 cut(s) 722
HinfI GANTC 5 cut(s) 183, 238, 260, 309, 744
HpaII CCGG 3 cut(s) 267, 282, 893
HphI GGTGA 1 cut(s) 971
Hpy166II GTNNAC 2 cut(s) 991, 1028
Hpy188I TCNGA 6 cut(s) 165, 219, 243, 364, 426, 1006
Hpy8I GTNNAC 2 cut(s) 991, 1028
Hpy99I CGWCG 4 cut(s) 76, 169, 451, 612
HpyAV CCTTC 1 cut(s) 217
HpyCH4III ACNGT 7 cut(s) 76, 85, 380, 478, 553, 736, 983
HpyCH4IV ACGT 4 cut(s) 61, 398, 449, 610
HpyCH4V TGCA 4 cut(s) 597, 673, 739, 1001
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 107, 971
HpyF3I CTNAG 1 cut(s) 843
HpySE526I ACGT 4 cut(s) 61, 398, 449, 610
Hsp92I GRCGYC 1 cut(s) 276
Hsp92II CATG 3 cut(s) 393, 440, 691
HspAI GCGC 1 cut(s) 722
KpnI GGTACC 1 cut(s) 814
Ksp22I TGATCA 1 cut(s) 729
Kzo9I GATC 4 cut(s) 195, 421, 729, 828
LguI GCTCTTC 1 cut(s) 648
LmnI GCTCC 5 cut(s) 20, 166, 895, 1045, 1051
Lsp1109I GCAGC 1 cut(s) 569
LweI GCATC 5 cut(s) 442, 531, 682, 919, 1010
MaeI CTAG 2 cut(s) 156, 189
MaeII ACGT 4 cut(s) 61, 398, 449, 610
MaeIII GTNAC 4 cut(s) 133, 585, 770, 869
MalI GATC 4 cut(s) 197, 423, 731, 830
MboI GATC 4 cut(s) 195, 421, 729, 828
MfeI CAATTG 1 cut(s) 1050
MflI RGATCY 1 cut(s) 421
MhlI GDGCHC 1 cut(s) 163
MluCI AATT 1 cut(s) 1050
MlyI GAGTC 1 cut(s) 753
MmeI TCCRAC 3 cut(s) 188, 467, 556
Mph1103I ATGCAT 1 cut(s) 675
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 3 cut(s) 480, 636, 776
MslI CAYNNNNRTG 2 cut(s) 981, 984
MspI CCGG 3 cut(s) 267, 282, 893
MspR9I CCNGG 2 cut(s) 129, 567
MunI CAATTG 1 cut(s) 1050
Mva1269I GAATGC 1 cut(s) 675
MvaI CCWGG 2 cut(s) 129, 567
MvnI CGCG 4 cut(s) 15, 236, 314, 698
MwoI GCNNNNNNNGC 3 cut(s) 29, 107, 971
NcoI CCATGG 1 cut(s) 389
NdeII GATC 4 cut(s) 195, 421, 729, 828
NheI GCTAGC 1 cut(s) 188
NlaIII CATG 3 cut(s) 393, 440, 691
NlaIV GGNNCC 2 cut(s) 22, 812
NruI TCGCGA 2 cut(s) 314, 698
NsiI ATGCAT 1 cut(s) 675
PciSI GCTCTTC 1 cut(s) 648
PctI GAATGC 1 cut(s) 675
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 4 cut(s) 183, 238, 260, 309
Pfl23II CGTACG 1 cut(s) 80
PinAI ACCGGT 1 cut(s) 266
PkrI GCNGC 4 cut(s) 31, 52, 280, 559
PleI GAGTC 1 cut(s) 752
PpsI GAGTC 1 cut(s) 752
Psp124BI GAGCTC 1 cut(s) 163
Psp6I CCWGG 2 cut(s) 127, 565
PspGI CCWGG 2 cut(s) 127, 565
PspLI CGTACG 1 cut(s) 80
PspN4I GGNNCC 2 cut(s) 22, 812
PspPI GGNCC 2 cut(s) 56, 76
PstNI CAGNNNCTG 1 cut(s) 566
PsuI RGATCY 1 cut(s) 421
RruI TCGCGA 2 cut(s) 314, 698
RsaI GTAC 4 cut(s) 82, 564, 713, 812
RsaNI GTAC 4 cut(s) 81, 563, 712, 811
RseI CAYNNNNRTG 2 cut(s) 981, 984
Rsr2I CGGWCCG 1 cut(s) 76
RsrII CGGWCCG 1 cut(s) 76
SacI GAGCTC 1 cut(s) 163
SapI GCTCTTC 1 cut(s) 648
SaqAI TTAA 3 cut(s) 480, 636, 776
SatI GCNGC 4 cut(s) 30, 51, 279, 558
Sau3AI GATC 4 cut(s) 195, 421, 729, 828
Sau96I GGNCC 2 cut(s) 56, 76
SchI GAGTC 1 cut(s) 753
ScrFI CCNGG 2 cut(s) 129, 567
SduI GDGCHC 1 cut(s) 163
SfaNI GCATC 5 cut(s) 442, 531, 682, 919, 1010
SfcI CTRYAG 1 cut(s) 552
SgrAI CRCCGGYG 1 cut(s) 266
SinI GGWCC 2 cut(s) 56, 76
SmiMI CAYNNNNRTG 2 cut(s) 981, 984
SmlI CTYRAG 2 cut(s) 647, 851
SmoI CTYRAG 2 cut(s) 647, 851
Sse9I AATT 1 cut(s) 1050
SsiI CCGC 4 cut(s) 15, 29, 50, 279
SspMI CTAG 2 cut(s) 156, 189
SstI GAGCTC 1 cut(s) 163
StyD4I CCNGG 2 cut(s) 127, 565
StyI CCWWGG 4 cut(s) 24, 389, 766, 1018
TaaI ACNGT 7 cut(s) 76, 85, 380, 478, 553, 736, 983
TaiI ACGT 4 cut(s) 64, 401, 452, 613
TaqI TCGA 3 cut(s) 71, 532, 607
TasI AATT 1 cut(s) 1050
TauI GCSGC 3 cut(s) 32, 53, 281
TfiI GAWTC 4 cut(s) 183, 238, 260, 309
Tru1I TTAA 3 cut(s) 480, 636, 776
Tru9I TTAA 3 cut(s) 480, 636, 776
TscAI CASTG 2 cut(s) 599, 739
TseI GCWGC 1 cut(s) 557
TspDTI ATGAA 2 cut(s) 236, 676
TspGWI ACGGA 1 cut(s) 68
TspRI CASTG 2 cut(s) 599, 739
VpaK11BI GGWCC 2 cut(s) 56, 76
XcmI CCANNNNNNNNNTGG 1 cut(s) 521
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 2 cut(s) 156, 189
Zsp2I ATGCAT 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.