Rroxscaffold_3G00245650

Belongs to the alternative oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006091
Physical Location & Seq
Forward (+)
38694544 .. 38696054
1511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00245650.1

Sequence Viewer

Length: 1092 bp
ATGATGACGATGACTGGCACGCAAGCGTGCAGGCACATGGCGAAGTCGGCAGCGCACGTAGCAAGTGCGCGCCTCTTCTCCACGGCAGCCAGAGCTGCCACCACTAATGCGCCCACGGCCAAACAATTGTCCTCCATAATCACTCCTCTAAAGGATGCGGCCGCGGGCGCATGGTGGAACAACGGCAGCATCATTTACCCCGTTCAGAGGGTACGCAGTGGTAGCACTCTGGCTCTCGGCCGTCAGGAGAAGCAGCAGGAGGAGGCTGATCATAGCAACTCCGCCGTTTCGGTTGCCGGACACGGTGGTGGTAATAAGGACGATGAGGAGAAGGCCATCACCAGCTACTGGGGGGTGGGACCGAACAAGATTACCAAGGAAGACGGTACTGAATGGAAATGGAATTGCTTCAGACCATGGGAGACGTACAAGGCCGACACCTCGATAGATCTCCAGAAGCATCATGTGCCTACAACTTTGCTGGACAAAATGGCCTACTGGACTGTCAAGTCTCTCCGATGGCCTACGGACCTATTCTTCCAGAGGCGATATGGGTGCAGGGCAATGATGCTGGAGACAGTGGCAGCAGTGCCTGGAATGGTAGGAGGGATGCTGCTGCACTGCAAATCGCTTAGGCGATTCGAGCACAGTGGTGGATGGATCAAAGCTCTTCTAGAAGAAGCCGAGAACGAGCGTATGCACCTCATGACCTTCATGGAAGTCGCCAAGCCCCGGTGGTACGAGCGTGCACTTGTATTTGCCGTTCAAGGTGTTTTCTTCAATGCGTACTTCTTGGGATATATGATTTCACCCAAGTTTGCTCATAGAATGGTTGGATATCTTGAGGAGGAAGCAATTCACTCATATACCGAGTTCCTCAAGGAATTAGACAAAGGTAACATTGAGAATGTTCCAGCTCCAGCTATTGCTATTGATTATTGGCAGTTACCTTCCAATTCCACCTTGAGAGATGTTGTTACAGTTGTTAGGGCAGACGAGGCTCACCACCGAGATGTCAATCACTTCGCTTCGGACATACATTATAATGGACGTGAACTGAAGGAATCTCCAGCTCCAATTGGTTACCATTGA

Protein Analysis

363

Amino Acids

40.91

Weight (kDa)

7.77

Isoelectric Point (pI)

42.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 152 - 344 8e-84 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1044
AasI GACNNNNNNGTC 1 cut(s) 508
AccB7I CCANNNNNTGG 1 cut(s) 348
AccII CGCG 2 cut(s) 70, 164
AciI CCGC 4 cut(s) 158, 162, 164, 282
AclWI GGATC 1 cut(s) 668
AcoI YGGCCR 3 cut(s) 117, 159, 238
AcuI CTGAAG 2 cut(s) 394, 1079
AfaI GTAC 5 cut(s) 213, 388, 428, 740, 788
AfiI CCNNNNNNNGG 3 cut(s) 207, 348, 732
AgsI TTSAA 2 cut(s) 767, 781
AjiI CACGTC 1 cut(s) 1052
AjnI CCWGG 1 cut(s) 592
AleI CACNNNNGTG 2 cut(s) 306, 651
AluBI AGCT 6 cut(s) 95, 345, 668, 917, 923, 1073
AluI AGCT 6 cut(s) 95, 345, 668, 917, 923, 1073
Alw21I GWGCWC 2 cut(s) 648, 751
Alw26I GTCTC 3 cut(s) 416, 516, 569
Alw44I GTGCAC 1 cut(s) 747
AlwI GGATC 1 cut(s) 668
AlwNI CAGNNNCTG 2 cut(s) 348, 593
AoxI GGCC 7 cut(s) 117, 159, 238, 333, 432, 492, 521
ApaLI GTGCAC 1 cut(s) 747
ApeKI GCWGC 8 cut(s) 50, 86, 95, 186, 253, 584, 613, 616
Asp700I GAANNNNTTC 2 cut(s) 407, 855
AspLEI GCGC 5 cut(s) 55, 70, 72, 112, 170
AspS9I GGNCC 2 cut(s) 359, 529
AsuC2I CCSGG 1 cut(s) 733
AsuHPI GGTGA 3 cut(s) 331, 801, 995
AvaII GGWCC 2 cut(s) 359, 529
BaeGI GKGCMC 1 cut(s) 751
BaeI ACNNNNGTAYC 2 cut(s) 730, 763
BbsI GAAGAC 1 cut(s) 387
Bbv12I GWGCWC 2 cut(s) 648, 751
BbvI GCAGC 8 cut(s) 62, 82, 98, 198, 265, 596, 600, 603
BccI CCATC 3 cut(s) 344, 513, 651
BceAI ACGGC 6 cut(s) 99, 132, 199, 225, 269, 746
BcgI CGANNNNNNTGC 2 cut(s) 537, 571
BciT130I CCWGG 1 cut(s) 594
BclI TGATCA 1 cut(s) 268
BcnI CCSGG 1 cut(s) 733
BcoDI GTCTC 3 cut(s) 416, 516, 569
BfaI CTAG 1 cut(s) 674
BglII AGATCT 1 cut(s) 448
Bme1390I CCNGG 2 cut(s) 594, 733
Bme18I GGWCC 2 cut(s) 359, 529
BmgBI CACGTC 1 cut(s) 1052
BmgT120I GGNCC 2 cut(s) 359, 529
BmiI GGNNCC 1 cut(s) 360
BmrFI CCNGG 2 cut(s) 594, 733
BmrI ACTGGG 1 cut(s) 358
BmsI GCATC 5 cut(s) 145, 198, 469, 558, 600
BmuI ACTGGG 1 cut(s) 358
BpiI GAAGAC 1 cut(s) 387
BpmI CTGGAG 4 cut(s) 437, 593, 903, 1053
Bpu10I CCTNAGC 1 cut(s) 632
BpuEI CTTGAG 3 cut(s) 863, 863, 985
BpuMI CCSGG 1 cut(s) 733
BsaAI YACGTR 1 cut(s) 58
BsaBI GATNNNNATC 1 cut(s) 1017
BsaJI CCNNGG 6 cut(s) 81, 114, 162, 375, 416, 731
Bsc4I CCNNNNNNNGG 3 cut(s) 207, 348, 732
Bse1I ACTGG 3 cut(s) 19, 353, 503
Bse3DI GCAATG 1 cut(s) 570
Bse8I GATNNNNATC 1 cut(s) 1017
BseBI CCWGG 1 cut(s) 594
BseDI CCNNGG 6 cut(s) 81, 114, 162, 375, 416, 731
BseGI GGATG 3 cut(s) 160, 615, 662
BseJI GATNNNNATC 1 cut(s) 1017
BseLI CCNNNNNNNGG 3 cut(s) 207, 348, 732
BseMI GCAATG 1 cut(s) 570
BseNI ACTGG 3 cut(s) 19, 353, 503
BsePI GCGCGC 1 cut(s) 68
BseRI GAGGAG 4 cut(s) 135, 275, 341, 860
BseSI GKGCMC 1 cut(s) 751
BseX3I CGGCCG 2 cut(s) 159, 238
BseXI GCAGC 8 cut(s) 62, 82, 98, 198, 265, 596, 600, 603
BsgI GTGCAG 3 cut(s) 49, 577, 602
Bsh1236I CGCG 2 cut(s) 70, 164
Bsh1285I CGRYCG 2 cut(s) 162, 241
BshFI GGCC 7 cut(s) 119, 161, 240, 335, 434, 494, 523
BsiEI CGRYCG 2 cut(s) 162, 241
BsiHKAI GWGCWC 2 cut(s) 648, 751
BsiSI CCGG 2 cut(s) 297, 733
BslFI GGGAC 1 cut(s) 372
BslI CCNNNNNNNGG 3 cut(s) 207, 348, 732
BsmAI GTCTC 3 cut(s) 416, 516, 569
BsmBI CGTCTC 1 cut(s) 416
BsmFI GGGAC 1 cut(s) 372
BsnI GGCC 7 cut(s) 119, 161, 240, 335, 434, 494, 523
Bsp1286I GDGCHC 2 cut(s) 648, 751
Bsp143I GATC 3 cut(s) 268, 448, 660
Bsp19I CCATGG 1 cut(s) 416
BspACI CCGC 4 cut(s) 158, 162, 164, 282
BspANI GGCC 7 cut(s) 119, 161, 240, 335, 434, 494, 523
BspFNI CGCG 2 cut(s) 70, 164
BspHI TCATGA 1 cut(s) 705
BspLI GGNNCC 1 cut(s) 360
BspPI GGATC 1 cut(s) 668
BspQI GCTCTTC 1 cut(s) 675
BsrDI GCAATG 1 cut(s) 570
BsrI ACTGG 3 cut(s) 19, 353, 503
BssECI CCNNGG 6 cut(s) 81, 114, 162, 375, 416, 731
BssHII GCGCGC 1 cut(s) 68
BssMI GATC 3 cut(s) 268, 448, 660
BssT1I CCWWGG 2 cut(s) 375, 416
Bst2UI CCWGG 1 cut(s) 594
Bst4CI ACNGT 6 cut(s) 305, 386, 505, 580, 650, 982
Bst6I CTCTTC 2 cut(s) 80, 675
BstAPI GCANNNNNTGC 1 cut(s) 466
BstBAI YACGTR 1 cut(s) 58
BstC8I GCNNGC 7 cut(s) 20, 24, 28, 32, 70, 166, 747
BstDEI CTNAG 1 cut(s) 632
BstDSI CCRYGG 4 cut(s) 81, 114, 162, 416
BstEII GGTNACC 1 cut(s) 1082
BstF5I GGATG 3 cut(s) 160, 615, 662
BstFNI CGCG 2 cut(s) 70, 164
BstHHI GCGC 5 cut(s) 55, 70, 72, 112, 170
BstKTI GATC 3 cut(s) 271, 451, 663
BstMAI GTCTC 3 cut(s) 416, 516, 569
BstMBI GATC 3 cut(s) 268, 448, 660
BstMCI CGRYCG 2 cut(s) 162, 241
BstNI CCWGG 1 cut(s) 594
BstPI GGTNACC 1 cut(s) 1082
BstSCI CCNGG 2 cut(s) 592, 731
BstSLI GKGCMC 1 cut(s) 751
BstUI CGCG 2 cut(s) 70, 164
BstV1I GCAGC 8 cut(s) 62, 82, 98, 198, 265, 596, 600, 603
BstV2I GAAGAC 1 cut(s) 387
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BstZI CGGCCG 2 cut(s) 159, 238
BsuRI GGCC 7 cut(s) 119, 161, 240, 335, 434, 494, 523
BtgI CCRYGG 4 cut(s) 81, 114, 162, 416
BtrI CACGTC 1 cut(s) 1052
BtsCI GGATG 3 cut(s) 160, 615, 662
BtsI GCAGTG 3 cut(s) 223, 594, 619
BtsIMutI CAGTG 5 cut(s) 223, 585, 594, 619, 655
Cac8I GCNNGC 7 cut(s) 20, 24, 28, 32, 70, 166, 747
CaiI CAGNNNCTG 2 cut(s) 348, 593
CciI TCATGA 1 cut(s) 705
CciNI GCGGCCGC 1 cut(s) 159
CfoI GCGC 5 cut(s) 55, 70, 72, 112, 170
Cfr13I GGNCC 2 cut(s) 359, 529
Cfr42I CCGCGG 1 cut(s) 165
Csp6I GTAC 5 cut(s) 212, 387, 427, 739, 787
CviAII CATG 6 cut(s) 37, 171, 417, 464, 706, 715
CviQI GTAC 5 cut(s) 212, 387, 427, 739, 787
DdeI CTNAG 1 cut(s) 632
DpnI GATC 3 cut(s) 270, 450, 662
DpnII GATC 3 cut(s) 268, 448, 660
DrdI GACNNNNNNGTC 1 cut(s) 508
DseDI GACNNNNNNGTC 1 cut(s) 508
EaeI YGGCCR 3 cut(s) 117, 159, 238
EagI CGGCCG 2 cut(s) 159, 238
Eam1104I CTCTTC 2 cut(s) 80, 675
EarI CTCTTC 2 cut(s) 80, 675
EciI GGCGGA 1 cut(s) 271
EclXI CGGCCG 2 cut(s) 159, 238
Eco130I CCWWGG 2 cut(s) 375, 416
Eco32I GATATC 1 cut(s) 839
Eco47I GGWCC 2 cut(s) 359, 529
Eco52I CGGCCG 2 cut(s) 159, 238
Eco57I CTGAAG 2 cut(s) 394, 1079
Eco91I GGTNACC 1 cut(s) 1082
EcoO65I GGTNACC 1 cut(s) 1082
EcoRII CCWGG 1 cut(s) 592
EcoRV GATATC 1 cut(s) 839
EcoT14I CCWWGG 2 cut(s) 375, 416
ErhI CCWWGG 2 cut(s) 375, 416
Esp3I CGTCTC 1 cut(s) 416
FaeI CATG 6 cut(s) 40, 174, 420, 467, 709, 718
FaqI GGGAC 1 cut(s) 372
FatI CATG 6 cut(s) 36, 170, 416, 463, 705, 714
FauI CCCGC 1 cut(s) 157
FbaI TGATCA 1 cut(s) 268
FokI GGATG 3 cut(s) 167, 622, 669
FspBI CTAG 1 cut(s) 674
GlaI GCGC 5 cut(s) 54, 69, 71, 111, 169
GsuI CTGGAG 4 cut(s) 437, 593, 903, 1053
HaeIII GGCC 7 cut(s) 119, 161, 240, 335, 434, 494, 523
HapII CCGG 2 cut(s) 297, 733
HhaI GCGC 5 cut(s) 55, 70, 72, 112, 170
Hin1II CATG 6 cut(s) 40, 174, 420, 467, 709, 718
Hin6I GCGC 5 cut(s) 53, 68, 70, 110, 168
HinP1I GCGC 5 cut(s) 53, 68, 70, 110, 168
HinfI GANTC 2 cut(s) 639, 1064
HpaII CCGG 2 cut(s) 297, 733
HphI GGTGA 3 cut(s) 331, 801, 995
Hpy166II GTNNAC 2 cut(s) 749, 1055
Hpy188I TCNGA 4 cut(s) 207, 413, 518, 1033
Hpy188III TCNNGA 6 cut(s) 245, 454, 541, 674, 706, 842
Hpy8I GTNNAC 2 cut(s) 749, 1055
HpyAV CCTTC 4 cut(s) 325, 721, 960, 1054
HpyCH4III ACNGT 6 cut(s) 305, 386, 505, 580, 650, 982
HpyCH4IV ACGT 3 cut(s) 57, 425, 1051
HpyCH4V TGCA 6 cut(s) 30, 558, 619, 624, 700, 749
HpyF3I CTNAG 1 cut(s) 632
HpySE526I ACGT 3 cut(s) 57, 425, 1051
Hsp92II CATG 6 cut(s) 40, 174, 420, 467, 709, 718
HspAI GCGC 5 cut(s) 53, 68, 70, 110, 168
Ksp22I TGATCA 1 cut(s) 268
KspI CCGCGG 1 cut(s) 165
Kzo9I GATC 3 cut(s) 268, 448, 660
LguI GCTCTTC 1 cut(s) 675
LmnI GCTCC 2 cut(s) 922, 1078
Lsp1109I GCAGC 8 cut(s) 62, 82, 98, 198, 265, 596, 600, 603
LweI GCATC 5 cut(s) 145, 198, 469, 558, 600
MaeI CTAG 1 cut(s) 674
MaeII ACGT 3 cut(s) 57, 425, 1051
MaeIII GTNAC 4 cut(s) 896, 945, 976, 1082
MalI GATC 3 cut(s) 270, 450, 662
MboI GATC 3 cut(s) 268, 448, 660
MboII GAAGA 6 cut(s) 67, 392, 529, 662, 689, 769
MfeI CAATTG 2 cut(s) 125, 1077
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 2 cut(s) 648, 751
MluCI AATT 6 cut(s) 125, 403, 855, 884, 955, 1077
MmeI TCCRAC 1 cut(s) 814
MroXI GAANNNNTTC 2 cut(s) 407, 855
MslI CAYNNNNRTG 4 cut(s) 306, 651, 1011, 1044
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 2 cut(s) 297, 733
MspR9I CCNGG 2 cut(s) 594, 733
MunI CAATTG 2 cut(s) 125, 1077
MvaI CCWGG 1 cut(s) 594
MvnI CGCG 2 cut(s) 70, 164
NciI CCSGG 1 cut(s) 733
NcoI CCATGG 1 cut(s) 416
NdeII GATC 3 cut(s) 268, 448, 660
NlaIII CATG 6 cut(s) 40, 174, 420, 467, 709, 718
NlaIV GGNNCC 1 cut(s) 360
NmeAIII GCCGAG 2 cut(s) 216, 709
NotI GCGGCCGC 1 cut(s) 159
OliI CACNNNNGTG 2 cut(s) 306, 651
PagI TCATGA 1 cut(s) 705
PauI GCGCGC 1 cut(s) 68
PciSI GCTCTTC 1 cut(s) 675
PdmI GAANNNNTTC 2 cut(s) 407, 855
PfeI GAWTC 2 cut(s) 639, 1064
PflMI CCANNNNNTGG 1 cut(s) 348
Ppu21I YACGTR 1 cut(s) 58
PsiI TTATAA 1 cut(s) 1044
Psp6I CCWGG 1 cut(s) 592
PspEI GGTNACC 1 cut(s) 1082
PspGI CCWGG 1 cut(s) 592
PspN4I GGNNCC 1 cut(s) 360
PspPI GGNCC 2 cut(s) 359, 529
PsrI GAACNNNNNNTAC 2 cut(s) 747, 779
PstNI CAGNNNCTG 2 cut(s) 348, 593
PsuI RGATCY 1 cut(s) 448
PteI GCGCGC 1 cut(s) 68
RsaI GTAC 5 cut(s) 213, 388, 428, 740, 788
RsaNI GTAC 5 cut(s) 212, 387, 427, 739, 787
RseI CAYNNNNRTG 4 cut(s) 306, 651, 1011, 1044
SacII CCGCGG 1 cut(s) 165
SapI GCTCTTC 1 cut(s) 675
Sau3AI GATC 3 cut(s) 268, 448, 660
Sau96I GGNCC 2 cut(s) 359, 529
ScrFI CCNGG 2 cut(s) 594, 733
SduI GDGCHC 2 cut(s) 648, 751
SfaNI GCATC 5 cut(s) 145, 198, 469, 558, 600
Sfr303I CCGCGG 1 cut(s) 165
SgrBI CCGCGG 1 cut(s) 165
SinI GGWCC 2 cut(s) 359, 529
SmiMI CAYNNNNRTG 4 cut(s) 306, 651, 1011, 1044
SmlI CTYRAG 3 cut(s) 842, 878, 964
SmoI CTYRAG 3 cut(s) 842, 878, 964
Sse9I AATT 6 cut(s) 125, 403, 855, 884, 955, 1077
SsiI CCGC 4 cut(s) 158, 162, 164, 282
SspMI CTAG 1 cut(s) 674
StyD4I CCNGG 2 cut(s) 592, 731
StyI CCWWGG 2 cut(s) 375, 416
TaaI ACNGT 6 cut(s) 305, 386, 505, 580, 650, 982
TaiI ACGT 3 cut(s) 60, 428, 1054
TaqI TCGA 2 cut(s) 443, 642
TaqII GACCGA 1 cut(s) 376
TasI AATT 6 cut(s) 125, 403, 855, 884, 955, 1077
TauI GCSGC 2 cut(s) 161, 164
TfiI GAWTC 2 cut(s) 639, 1064
TscAI CASTG 5 cut(s) 223, 585, 594, 626, 655
TseI GCWGC 8 cut(s) 50, 86, 95, 186, 253, 584, 613, 616
TspDTI ATGAA 1 cut(s) 703
TspGWI ACGGA 1 cut(s) 542
TspRI CASTG 5 cut(s) 223, 585, 594, 626, 655
Van91I CCANNNNNTGG 1 cut(s) 348
VneI GTGCAC 1 cut(s) 747
VpaK11BI GGWCC 2 cut(s) 359, 529
XbaI TCTAGA 1 cut(s) 673
XcmI CCANNNNNNNNNTGG 1 cut(s) 548
XmnI GAANNNNTTC 2 cut(s) 407, 855
XspI CTAG 1 cut(s) 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.