FvH4_5g24160

Belongs to the alternative oxidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006091
Physical Location & Seq
Reverse (-)
15438581 .. 15440434
1854 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g24160.t1

Sequence Viewer

Length: 1092 bp
ATGATGGCGATGACTCGCACGCAGGCGTGCAGGTACATGGTCAAGTCGGCGGCGCACGTAGCAAGTGCCCGCCTCTTCTCCACGGCAGCAAGAGCTGCCACCACCAATGCGCCCGCGGCCAAACAATTGTCCTCCGTTATCATTCCTCTTAAGGACATGGCCGCGAGGGCACGGTGGAACAATAGCAGCATCATTTACCCCGTTCAGAAGGTCCGCAGTGCTAGCACTCTGGCTCTCGGTCATCAGGAGAAGCAGCACGAGGAGGCTGATCGTAGCAACTCCACTGTCTTGGTTGCCGGACAAGGTGGTGGTAAAAAGGATGATGAGGAGAATGGGATTACCAGCTACTGGGGGGTGGCACCGAACAAGATTACCAAGGAAGACGGTACTGAATGGAAATGGAACTGCTTCAGACCATGGGAGACGTACAAAGCCGACACCTCCATAGATCTCCAGAAGCATCACCTGCCTACTACTTTCATGGACAAAGTGGCCTACTGGACGGTCAAGTCTCTCCGATGGCCTACCGACCTATTTTTCCAGAGGCGATATGGGTGCAGGGCAATGATGCTGGAGACAGTGGCAGCAGTGCCTGGGATGGTAGGAGGGATGCTGCTGCACTGCAAGTCCCTTAGGCGATTTGAGCACAGCGGCGGCTGGATCAAAGCTCTTCTTGAAGAAGCCGAGAACGAGCGCATGCACCTCATGACCTTCATGGAAGTCGCCAAGCCCAGGTGGTACGAGCGTGCCCTCGTCTTTGCAGTTCAAGGTGTCTTCTTCAATGCTTACTTCTTGGGATATATGATTTCGCCAAAGTTTGCTCATAGAATGGTTGGGTATCTTGAGGAGGAAGCAATTCACTCATATACTGAGTTCCTCAAGGAATTGGACAAAGGTAACATTGAGAATGTTCCTGCTCCAGCCATCGCTATTGATTATTGGCAGCTACCACCCAATTCCACCTTGAGAGATGTTGTTACAGTTGTTAGGGCAGACGAGGCTCACCACCGAGATGTCAATCACTTTGCTTCGGACATACATTATAATGGACGTGAACTGAAGGAATCTCCAGCTCCAATTGGTTACCATTGA

Protein Analysis

364

Amino Acids

41.25

Weight (kDa)

8.71

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AOX PF01786 153 - 344 3.8e-84 Alternative oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1044
AarI CACCTGC 1 cut(s) 474
AasI GACNNNNNNGTC 1 cut(s) 508
Acc36I ACCTGC 2 cut(s) 21, 474
AccB1I GGYRCC 1 cut(s) 358
AccB7I CCANNNNNTGG 1 cut(s) 348
AccII CGCG 2 cut(s) 116, 164
AciI CCGC 8 cut(s) 50, 70, 114, 116, 162, 214, 651, 654
AclWI GGATC 1 cut(s) 668
AcoI YGGCCR 2 cut(s) 117, 159
AcuI CTGAAG 2 cut(s) 394, 1079
AfaI GTAC 4 cut(s) 35, 388, 428, 740
AfiI CCNNNNNNNGG 2 cut(s) 348, 732
AflII CTTAAG 1 cut(s) 149
AgsI TTSAA 3 cut(s) 677, 767, 781
AjiI CACGTC 1 cut(s) 1052
AjnI CCWGG 2 cut(s) 592, 731
AluBI AGCT 5 cut(s) 95, 345, 668, 946, 1073
AluI AGCT 5 cut(s) 95, 345, 668, 946, 1073
Alw21I GWGCWC 1 cut(s) 648
Alw26I GTCTC 3 cut(s) 416, 516, 569
AlwI GGATC 1 cut(s) 668
AlwNI CAGNNNCTG 2 cut(s) 348, 593
AoxI GGCC 4 cut(s) 117, 159, 492, 521
ApeKI GCWGC 8 cut(s) 86, 95, 186, 253, 584, 613, 616, 943
Asp700I GAANNNNTTC 2 cut(s) 407, 855
AspLEI GCGC 3 cut(s) 55, 112, 696
AspS9I GGNCC 1 cut(s) 211
AsuHPI GGTGA 2 cut(s) 455, 995
AsuNHI GCTAGC 1 cut(s) 221
AvaII GGWCC 1 cut(s) 211
AxyI CCTNAGG 1 cut(s) 632
BaeGI GKGCMC 3 cut(s) 70, 172, 751
BaeI ACNNNNGTAYC 2 cut(s) 730, 763
BanI GGYRCC 1 cut(s) 358
BauI CACGAG 1 cut(s) 257
BbsI GAAGAC 2 cut(s) 387, 766
Bbv12I GWGCWC 1 cut(s) 648
BbvI GCAGC 8 cut(s) 82, 98, 198, 265, 596, 600, 603, 955
BccI CCATC 3 cut(s) 513, 592, 932
BceAI ACGGC 1 cut(s) 99
BcgI CGANNNNNNTGC 2 cut(s) 537, 571
BciT130I CCWGG 2 cut(s) 594, 733
BcoDI GTCTC 3 cut(s) 416, 516, 569
BfaI CTAG 1 cut(s) 222
BfrI CTTAAG 1 cut(s) 149
BfuAI ACCTGC 2 cut(s) 21, 474
BglI GCCNNNNNGGC 1 cut(s) 167
BglII AGATCT 1 cut(s) 448
Bme1390I CCNGG 2 cut(s) 594, 733
Bme18I GGWCC 1 cut(s) 211
BmgBI CACGTC 1 cut(s) 1052
BmgT120I GGNCC 1 cut(s) 211
BmiI GGNNCC 1 cut(s) 360
BmrFI CCNGG 2 cut(s) 594, 733
BmrI ACTGGG 1 cut(s) 358
BmsI GCATC 4 cut(s) 198, 469, 558, 600
BmtI GCTAGC 1 cut(s) 225
BmuI ACTGGG 1 cut(s) 358
BpiI GAAGAC 2 cut(s) 387, 766
BpmI CTGGAG 4 cut(s) 437, 593, 903, 1053
BpuEI CTTGAG 3 cut(s) 863, 863, 985
BsaAI YACGTR 1 cut(s) 58
BsaBI GATNNNNATC 1 cut(s) 1017
BsaJI CCNNGG 6 cut(s) 81, 114, 375, 416, 593, 731
Bsc4I CCNNNNNNNGG 2 cut(s) 348, 732
Bse1I ACTGG 2 cut(s) 353, 503
Bse21I CCTNAGG 1 cut(s) 632
Bse3DI GCAATG 1 cut(s) 570
Bse8I GATNNNNATC 1 cut(s) 1017
BseBI CCWGG 2 cut(s) 594, 733
BseDI CCNNGG 6 cut(s) 81, 114, 375, 416, 593, 731
BseGI GGATG 3 cut(s) 325, 603, 615
BseJI GATNNNNATC 1 cut(s) 1017
BseLI CCNNNNNNNGG 2 cut(s) 348, 732
BseMI GCAATG 1 cut(s) 570
BseMII CTCAG 1 cut(s) 861
BseNI ACTGG 2 cut(s) 353, 503
BseRI GAGGAG 3 cut(s) 275, 341, 860
BseSI GKGCMC 3 cut(s) 70, 172, 751
BseXI GCAGC 8 cut(s) 82, 98, 198, 265, 596, 600, 603, 955
BsgI GTGCAG 3 cut(s) 49, 577, 602
Bsh1236I CGCG 2 cut(s) 116, 164
BshFI GGCC 4 cut(s) 119, 161, 494, 523
BshNI GGYRCC 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 648
BsiSI CCGG 1 cut(s) 297
BslFI GGGAC 1 cut(s) 613
BslI CCNNNNNNNGG 2 cut(s) 348, 732
BsmAI GTCTC 3 cut(s) 416, 516, 569
BsmBI CGTCTC 1 cut(s) 416
BsmFI GGGAC 1 cut(s) 613
BsnI GGCC 4 cut(s) 119, 161, 494, 523
Bsp1286I GDGCHC 4 cut(s) 70, 172, 648, 751
Bsp143I GATC 3 cut(s) 268, 448, 660
Bsp19I CCATGG 1 cut(s) 416
BspACI CCGC 8 cut(s) 50, 70, 114, 116, 162, 214, 651, 654
BspANI GGCC 4 cut(s) 119, 161, 494, 523
BspCNI CTCAG 1 cut(s) 862
BspFNI CGCG 2 cut(s) 116, 164
BspHI TCATGA 1 cut(s) 705
BspLI GGNNCC 1 cut(s) 360
BspMI ACCTGC 2 cut(s) 21, 474
BspOI GCTAGC 1 cut(s) 225
BspPI GGATC 1 cut(s) 668
BspQI GCTCTTC 1 cut(s) 675
BspT107I GGYRCC 1 cut(s) 358
BspTI CTTAAG 1 cut(s) 149
BsrDI GCAATG 1 cut(s) 570
BsrI ACTGG 2 cut(s) 353, 503
BssECI CCNNGG 6 cut(s) 81, 114, 375, 416, 593, 731
BssMI GATC 3 cut(s) 268, 448, 660
BssSI CACGAG 1 cut(s) 257
BssT1I CCWWGG 2 cut(s) 375, 416
Bst2BI CACGAG 1 cut(s) 257
Bst2UI CCWGG 2 cut(s) 594, 733
Bst4CI ACNGT 6 cut(s) 174, 286, 386, 505, 580, 982
Bst6I CTCTTC 2 cut(s) 80, 675
BstAFI CTTAAG 1 cut(s) 149
BstAPI GCANNNNNTGC 2 cut(s) 95, 466
BstBAI YACGTR 1 cut(s) 58
BstC8I GCNNGC 8 cut(s) 20, 24, 28, 70, 114, 223, 698, 747
BstDEI CTNAG 2 cut(s) 632, 870
BstDSI CCRYGG 3 cut(s) 81, 114, 416
BstEII GGTNACC 1 cut(s) 1082
BstF5I GGATG 3 cut(s) 325, 603, 615
BstFNI CGCG 2 cut(s) 116, 164
BstHHI GCGC 3 cut(s) 55, 112, 696
BstKTI GATC 3 cut(s) 271, 451, 663
BstMAI GTCTC 3 cut(s) 416, 516, 569
BstMBI GATC 3 cut(s) 268, 448, 660
BstMWI GCNNNNNNNGC 9 cut(s) 59, 92, 95, 116, 167, 222, 466, 643, 998
BstNI CCWGG 2 cut(s) 594, 733
BstNSI RCATGY 1 cut(s) 700
BstPI GGTNACC 1 cut(s) 1082
BstSCI CCNGG 2 cut(s) 592, 731
BstSLI GKGCMC 3 cut(s) 70, 172, 751
BstUI CGCG 2 cut(s) 116, 164
BstV1I GCAGC 8 cut(s) 82, 98, 198, 265, 596, 600, 603, 955
BstV2I GAAGAC 2 cut(s) 387, 766
BstX2I RGATCY 1 cut(s) 448
BstXI CCANNNNNNTGG 1 cut(s) 289
BstYI RGATCY 1 cut(s) 448
Bsu36I CCTNAGG 1 cut(s) 632
BsuRI GGCC 4 cut(s) 119, 161, 494, 523
BtgI CCRYGG 3 cut(s) 81, 114, 416
BtgZI GCGATG 2 cut(s) 23, 910
BtrI CACGTC 1 cut(s) 1052
BtsCI GGATG 3 cut(s) 325, 603, 615
BtsI GCAGTG 3 cut(s) 223, 594, 619
BtsIMutI CAGTG 5 cut(s) 223, 282, 585, 594, 619
BveI ACCTGC 2 cut(s) 21, 474
Cac8I GCNNGC 8 cut(s) 20, 24, 28, 70, 114, 223, 698, 747
CaiI CAGNNNCTG 2 cut(s) 348, 593
CciI TCATGA 1 cut(s) 705
CfoI GCGC 3 cut(s) 55, 112, 696
Cfr13I GGNCC 1 cut(s) 211
Cfr42I CCGCGG 1 cut(s) 117
Csp6I GTAC 4 cut(s) 34, 387, 427, 739
CviAII CATG 7 cut(s) 37, 157, 417, 481, 697, 706, 715
CviQI GTAC 4 cut(s) 34, 387, 427, 739
DdeI CTNAG 2 cut(s) 632, 870
DpnI GATC 3 cut(s) 270, 450, 662
DpnII GATC 3 cut(s) 268, 448, 660
DrdI GACNNNNNNGTC 1 cut(s) 508
DseDI GACNNNNNNGTC 1 cut(s) 508
EaeI YGGCCR 2 cut(s) 117, 159
Eam1104I CTCTTC 2 cut(s) 80, 675
EarI CTCTTC 2 cut(s) 80, 675
Eco130I CCWWGG 2 cut(s) 375, 416
Eco47I GGWCC 1 cut(s) 211
Eco57I CTGAAG 2 cut(s) 394, 1079
Eco81I CCTNAGG 1 cut(s) 632
Eco91I GGTNACC 1 cut(s) 1082
EcoO65I GGTNACC 1 cut(s) 1082
EcoRII CCWGG 2 cut(s) 592, 731
EcoT14I CCWWGG 2 cut(s) 375, 416
ErhI CCWWGG 2 cut(s) 375, 416
Esp3I CGTCTC 1 cut(s) 416
FaeI CATG 7 cut(s) 40, 160, 420, 484, 700, 709, 718
FalI AAGNNNNNCTT 2 cut(s) 657, 689
FaqI GGGAC 1 cut(s) 613
FatI CATG 7 cut(s) 36, 156, 416, 480, 696, 705, 714
FauI CCCGC 2 cut(s) 77, 121
FokI GGATG 3 cut(s) 332, 610, 622
FspBI CTAG 1 cut(s) 222
GlaI GCGC 3 cut(s) 54, 111, 695
GsuI CTGGAG 4 cut(s) 437, 593, 903, 1053
HaeIII GGCC 4 cut(s) 119, 161, 494, 523
HapII CCGG 1 cut(s) 297
HhaI GCGC 3 cut(s) 55, 112, 696
Hin1II CATG 7 cut(s) 40, 160, 420, 484, 700, 709, 718
Hin6I GCGC 3 cut(s) 53, 110, 694
HinP1I GCGC 3 cut(s) 53, 110, 694
HinfI GANTC 2 cut(s) 13, 1064
HpaII CCGG 1 cut(s) 297
HphI GGTGA 2 cut(s) 455, 995
Hpy166II GTNNAC 1 cut(s) 1055
Hpy188I TCNGA 4 cut(s) 207, 413, 518, 1033
Hpy188III TCNNGA 6 cut(s) 245, 454, 541, 674, 706, 842
Hpy8I GTNNAC 1 cut(s) 1055
HpyAV CCTTC 3 cut(s) 202, 721, 1054
HpyCH4III ACNGT 6 cut(s) 174, 286, 386, 505, 580, 982
HpyCH4IV ACGT 3 cut(s) 57, 425, 1051
HpyCH4V TGCA 6 cut(s) 30, 558, 619, 624, 700, 761
HpyF10VI GCNNNNNNNGC 9 cut(s) 59, 92, 95, 116, 167, 222, 466, 643, 998
HpyF3I CTNAG 2 cut(s) 632, 870
HpySE526I ACGT 3 cut(s) 57, 425, 1051
Hsp92II CATG 7 cut(s) 40, 160, 420, 484, 700, 709, 718
HspAI GCGC 3 cut(s) 53, 110, 694
KspI CCGCGG 1 cut(s) 117
Kzo9I GATC 3 cut(s) 268, 448, 660
LguI GCTCTTC 1 cut(s) 675
LmnI GCTCC 2 cut(s) 922, 1078
Lsp1109I GCAGC 8 cut(s) 82, 98, 198, 265, 596, 600, 603, 955
LweI GCATC 4 cut(s) 198, 469, 558, 600
MaeI CTAG 1 cut(s) 222
MaeII ACGT 3 cut(s) 57, 425, 1051
MaeIII GTNAC 3 cut(s) 896, 976, 1082
MalI GATC 3 cut(s) 270, 450, 662
MboI GATC 3 cut(s) 268, 448, 660
MboII GAAGA 6 cut(s) 67, 392, 662, 689, 766, 769
MfeI CAATTG 2 cut(s) 125, 1077
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 4 cut(s) 70, 172, 648, 751
MluCI AATT 5 cut(s) 125, 855, 884, 955, 1077
MlyI GAGTC 1 cut(s) 7
MroXI GAANNNNTTC 2 cut(s) 407, 855
MseI TTAA 1 cut(s) 150
MslI CAYNNNNRTG 2 cut(s) 1011, 1044
MspA1I CMGCKG 2 cut(s) 116, 651
MspCI CTTAAG 1 cut(s) 149
MspI CCGG 1 cut(s) 297
MspR9I CCNGG 2 cut(s) 594, 733
MunI CAATTG 2 cut(s) 125, 1077
MvaI CCWGG 2 cut(s) 594, 733
MvnI CGCG 2 cut(s) 116, 164
MwoI GCNNNNNNNGC 9 cut(s) 59, 92, 95, 116, 167, 222, 466, 643, 998
NcoI CCATGG 1 cut(s) 416
NdeII GATC 3 cut(s) 268, 448, 660
NheI GCTAGC 1 cut(s) 221
NlaIII CATG 7 cut(s) 40, 160, 420, 484, 700, 709, 718
NlaIV GGNNCC 1 cut(s) 360
NmeAIII GCCGAG 1 cut(s) 709
NspI RCATGY 1 cut(s) 700
PaeI GCATGC 1 cut(s) 700
PagI TCATGA 1 cut(s) 705
PaqCI CACCTGC 1 cut(s) 474
PciSI GCTCTTC 1 cut(s) 675
PdmI GAANNNNTTC 2 cut(s) 407, 855
PfeI GAWTC 1 cut(s) 1064
PflMI CCANNNNNTGG 1 cut(s) 348
PleI GAGTC 1 cut(s) 7
PpsI GAGTC 1 cut(s) 7
Ppu21I YACGTR 1 cut(s) 58
PsiI TTATAA 1 cut(s) 1044
Psp6I CCWGG 2 cut(s) 592, 731
PspEI GGTNACC 1 cut(s) 1082
PspGI CCWGG 2 cut(s) 592, 731
PspN4I GGNNCC 1 cut(s) 360
PspPI GGNCC 1 cut(s) 211
PstNI CAGNNNCTG 2 cut(s) 348, 593
PsuI RGATCY 1 cut(s) 448
RsaI GTAC 4 cut(s) 35, 388, 428, 740
RsaNI GTAC 4 cut(s) 34, 387, 427, 739
RseI CAYNNNNRTG 2 cut(s) 1011, 1044
SacII CCGCGG 1 cut(s) 117
SapI GCTCTTC 1 cut(s) 675
SaqAI TTAA 1 cut(s) 150
Sau3AI GATC 3 cut(s) 268, 448, 660
Sau96I GGNCC 1 cut(s) 211
SchI GAGTC 1 cut(s) 7
ScrFI CCNGG 2 cut(s) 594, 733
SduI GDGCHC 4 cut(s) 70, 172, 648, 751
SfaNI GCATC 4 cut(s) 198, 469, 558, 600
Sfr303I CCGCGG 1 cut(s) 117
SgrBI CCGCGG 1 cut(s) 117
SinI GGWCC 1 cut(s) 211
SmiMI CAYNNNNRTG 2 cut(s) 1011, 1044
SmlI CTYRAG 4 cut(s) 149, 842, 878, 964
SmoI CTYRAG 4 cut(s) 149, 842, 878, 964
SphI GCATGC 1 cut(s) 700
Sse9I AATT 5 cut(s) 125, 855, 884, 955, 1077
SsiI CCGC 8 cut(s) 50, 70, 114, 116, 162, 214, 651, 654
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 2 cut(s) 592, 731
StyI CCWWGG 2 cut(s) 375, 416
TaaI ACNGT 6 cut(s) 174, 286, 386, 505, 580, 982
TaiI ACGT 3 cut(s) 60, 428, 1054
TaqII GACCGA 1 cut(s) 227
TasI AATT 5 cut(s) 125, 855, 884, 955, 1077
TauI GCSGC 5 cut(s) 53, 119, 164, 654, 657
TfiI GAWTC 1 cut(s) 1064
Tru1I TTAA 1 cut(s) 150
Tru9I TTAA 1 cut(s) 150
TscAI CASTG 5 cut(s) 223, 289, 585, 594, 626
TseI GCWGC 8 cut(s) 86, 95, 186, 253, 584, 613, 616, 943
TspDTI ATGAA 2 cut(s) 469, 703
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 5 cut(s) 223, 289, 585, 594, 626
Van91I CCANNNNNTGG 1 cut(s) 348
Vha464I CTTAAG 1 cut(s) 149
VpaK11BI GGWCC 1 cut(s) 211
XceI RCATGY 1 cut(s) 700
XcmI CCANNNNNNNNNTGG 1 cut(s) 548
XmnI GAANNNNTTC 2 cut(s) 407, 855
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.