AT1G35680

Ribosomal protein L21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004346
Physical Location & Seq
Forward (+)
13208683 .. 13210428
1746 bp
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UTR
Exon/CDS
Intron
AT1G35680.1

Sequence Viewer

Length: 663 bp
ATGGCGTCTTCTTCAGCGACGCTCTCTCTCTGCTCTACTTTCTCTGCTCATTGCAATGTCAATTCTCGCCGTTCTAGCACTATCCTCTGTTCTCTCTCTAAACCTTCGCTTAATTTGGCTAAACCTCTGACTGGATTTCTTTCTCCTTCCACTGCGTCGACGTCTCGAACCGCATTCACCGTCGCTCCGAAATTCGCGGAATCAGTCGTCGAAGCTGAACCGGAGACTACCGATATCGAAGCTGTGGTGGTTTCCGATGTTTCGGAGGTTACTGAGGAAAAAGCTAAACGAGAAGAGATTTTCGCTGTTATTATGGTTGGTGGACGCCAATACATAGTGTTTCCAGGGAGATATCTCTACACTCAGAGGCTAAAAGATGCAAATGTTGATGACCAGATTGTTCTCAACAAAGTGTTGCTTGTTGGAACAAAGACACACACTTACATTGGCAAGCCGGTTGTGACCAATGCCACTGTACATGCTGTTGTGGAAAGTCAAGGTTTGAATGATAAAGTGGTAGTCTTCAAGTACAAGCCTAAGAAGAAGTACCGTAGAAATATCGGTCACAGACAGCCGAATACGAGGATTAGGATTACCGGAATCACAGGGTATGAAGAGTATCCAGCCTCGCCCAATGTTGCAGTTGGAGAAGTAAATCTTTGA

Protein Analysis

220

Amino Acids

24.04

Weight (kDa)

9.32

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L21p PF00829 101 - 201 1e-31 Ribosomal prokaryotic L21 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 164
AccI GTMKAC 1 cut(s) 158
AccII CGCG 1 cut(s) 197
AciI CCGC 2 cut(s) 171, 197
AcsI RAATTY 1 cut(s) 191
AcyI GRCGYC 3 cut(s) 5, 161, 325
AfaI GTAC 3 cut(s) 477, 530, 548
AfiI CCNNNNNNNGG 1 cut(s) 131
AgsI TTSAA 2 cut(s) 505, 526
AjnI CCWGG 1 cut(s) 343
AluBI AGCT 3 cut(s) 215, 242, 284
AluI AGCT 3 cut(s) 215, 242, 284
Alw26I GTCTC 2 cut(s) 168, 218
ApoI RAATTY 1 cut(s) 191
AsuHPI GGTGA 1 cut(s) 169
BbsI GAAGAC 1 cut(s) 514
BceAI ACGGC 1 cut(s) 54
BciT130I CCWGG 1 cut(s) 345
BciVI GTATCC 1 cut(s) 630
BcoDI GTCTC 2 cut(s) 168, 218
BfaI CTAG 1 cut(s) 75
BfuI GTATCC 1 cut(s) 630
Bme1390I CCNGG 1 cut(s) 345
BmrFI CCNGG 1 cut(s) 345
BmsI GCATC 1 cut(s) 367
BpiI GAAGAC 1 cut(s) 514
BsaHI GRCGYC 3 cut(s) 5, 161, 325
BsaJI CCNNGG 1 cut(s) 344
BsaWI WCCGGW 2 cut(s) 220, 596
BsaXI ACNNNNNCTCC 2 cut(s) 169, 199
Bsc4I CCNNNNNNNGG 1 cut(s) 131
Bse118I RCCGGY 1 cut(s) 454
Bse1I ACTGG 1 cut(s) 136
Bse3DI GCAATG 2 cut(s) 49, 61
BseBI CCWGG 1 cut(s) 345
BseDI CCNNGG 1 cut(s) 344
BseLI CCNNNNNNNGG 1 cut(s) 131
BseMI GCAATG 2 cut(s) 49, 61
BseMII CTCAG 2 cut(s) 264, 377
BseNI ACTGG 1 cut(s) 136
Bsh1236I CGCG 1 cut(s) 197
BsiSI CCGG 3 cut(s) 221, 455, 597
BslI CCNNNNNNNGG 1 cut(s) 131
BsmAI GTCTC 2 cut(s) 168, 218
BsmBI CGTCTC 1 cut(s) 168
BsmI GAATGC 1 cut(s) 173
Bsp1407I TGTACA 1 cut(s) 475
BspACI CCGC 2 cut(s) 171, 197
BspCNI CTCAG 2 cut(s) 265, 376
BspFNI CGCG 1 cut(s) 197
BsrDI GCAATG 2 cut(s) 49, 61
BsrFI RCCGGY 1 cut(s) 454
BsrGI TGTACA 1 cut(s) 475
BsrI ACTGG 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 454
BssECI CCNNGG 1 cut(s) 344
BssNI GRCGYC 3 cut(s) 5, 161, 325
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 3 cut(s) 181, 475, 551
Bst6I CTCTTC 2 cut(s) 288, 609
BstACI GRCGYC 3 cut(s) 5, 161, 325
BstAUI TGTACA 1 cut(s) 475
BstC8I GCNNGC 1 cut(s) 452
BstDEI CTNAG 3 cut(s) 273, 363, 537
BstFNI CGCG 1 cut(s) 197
BstMAI GTCTC 2 cut(s) 168, 218
BstMWI GCNNNNNNNGC 1 cut(s) 75
BstNI CCWGG 1 cut(s) 345
BstNSI RCATGY 1 cut(s) 482
BstSCI CCNGG 1 cut(s) 343
BstUI CGCG 1 cut(s) 197
BstV2I GAAGAC 1 cut(s) 514
BsuI GTATCC 1 cut(s) 630
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 2 cut(s) 150, 471
Cac8I GCNNGC 1 cut(s) 452
Cfr10I RCCGGY 1 cut(s) 454
CseI GACGC 3 cut(s) 28, 144, 333
Csp6I GTAC 3 cut(s) 476, 529, 547
CviAII CATG 1 cut(s) 479
CviJI RGCY 9 cut(s) 119, 215, 242, 284, 370, 454, 535, 574, 626
CviKI_1 RGCY 9 cut(s) 119, 215, 242, 284, 370, 454, 535, 574, 626
CviQI GTAC 3 cut(s) 476, 529, 547
DdeI CTNAG 3 cut(s) 273, 363, 537
Eam1104I CTCTTC 2 cut(s) 288, 609
EarI CTCTTC 2 cut(s) 288, 609
Eco32I GATATC 2 cut(s) 235, 353
EcoRII CCWGG 1 cut(s) 343
EcoRV GATATC 2 cut(s) 235, 353
Esp3I CGTCTC 1 cut(s) 168
FaeI CATG 1 cut(s) 482
FaiI YATR 4 cut(s) 314, 335, 480, 612
FalI AAGNNNNNCTT 3 cut(s) 402, 434, 642
FatI CATG 1 cut(s) 478
FblI GTMKAC 1 cut(s) 158
FspBI CTAG 1 cut(s) 75
HapII CCGG 3 cut(s) 221, 455, 597
HgaI GACGC 3 cut(s) 28, 144, 333
Hin1I GRCGYC 3 cut(s) 5, 161, 325
Hin1II CATG 1 cut(s) 482
HincII GTYRAC 1 cut(s) 159
HindII GTYRAC 1 cut(s) 159
HinfI GANTC 2 cut(s) 200, 600
HpaII CCGG 3 cut(s) 221, 455, 597
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 2 cut(s) 159, 323
Hpy188I TCNGA 5 cut(s) 129, 189, 256, 265, 366
Hpy188III TCNNGA 1 cut(s) 165
Hpy8I GTNNAC 2 cut(s) 159, 323
Hpy99I CGWCG 5 cut(s) 22, 160, 163, 185, 212
HpyAV CCTTC 2 cut(s) 114, 156
HpyCH4III ACNGT 3 cut(s) 181, 475, 551
HpyCH4IV ACGT 1 cut(s) 161
HpyCH4V TGCA 3 cut(s) 54, 380, 641
HpyF10VI GCNNNNNNNGC 1 cut(s) 75
HpyF3I CTNAG 3 cut(s) 273, 363, 537
HpySE526I ACGT 1 cut(s) 161
Hsp92I GRCGYC 3 cut(s) 5, 161, 325
Hsp92II CATG 1 cut(s) 482
LmnI GCTCC 1 cut(s) 190
LpnPI CCDG 9 cut(s) 117, 234, 330, 357, 407, 468, 591, 610, 636
LweI GCATC 1 cut(s) 367
MaeI CTAG 1 cut(s) 75
MaeII ACGT 1 cut(s) 161
MaeIII GTNAC 3 cut(s) 268, 460, 563
MboII GAAGA 5 cut(s) 3, 305, 514, 553, 626
MluCI AATT 3 cut(s) 61, 112, 191
MmeI TCCRAC 2 cut(s) 403, 625
MnlI CCTC 7 cut(s) 95, 135, 259, 268, 360, 576, 637
MseI TTAA 1 cut(s) 111
MslI CAYNNNNRTG 1 cut(s) 54
MspI CCGG 3 cut(s) 221, 455, 597
MspR9I CCNGG 1 cut(s) 345
Mva1269I GAATGC 1 cut(s) 173
MvaI CCWGG 1 cut(s) 345
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 1 cut(s) 75
NlaIII CATG 1 cut(s) 482
NmuCI GTSAC 2 cut(s) 460, 563
NspI RCATGY 1 cut(s) 482
PctI GAATGC 1 cut(s) 173
PfeI GAWTC 2 cut(s) 200, 600
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
RsaI GTAC 3 cut(s) 477, 530, 548
RsaNI GTAC 3 cut(s) 476, 529, 547
RseI CAYNNNNRTG 1 cut(s) 54
SalI GTCGAC 1 cut(s) 157
SaqAI TTAA 1 cut(s) 111
ScrFI CCNGG 1 cut(s) 345
SetI ASST 8 cut(s) 106, 127, 164, 217, 244, 270, 286, 502
SfaNI GCATC 1 cut(s) 367
SgrDI CGTCGACG 1 cut(s) 157
SmiMI CAYNNNNRTG 1 cut(s) 54
Sse9I AATT 3 cut(s) 61, 112, 191
SsiI CCGC 2 cut(s) 171, 197
SspMI CTAG 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 343
TaaI ACNGT 3 cut(s) 181, 475, 551
TaiI ACGT 1 cut(s) 164
TaqI TCGA 4 cut(s) 158, 166, 210, 237
TaqII GACCGA 1 cut(s) 551
TasI AATT 3 cut(s) 61, 112, 191
TatI WGTACW 2 cut(s) 475, 528
TfiI GAWTC 2 cut(s) 200, 600
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TscAI CASTG 2 cut(s) 157, 478
TseFI GTSAC 2 cut(s) 460, 563
Tsp45I GTSAC 2 cut(s) 460, 563
TspDTI ATGAA 1 cut(s) 627
TspRI CASTG 2 cut(s) 157, 478
XapI RAATTY 1 cut(s) 191
XceI RCATGY 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 158
XspI CTAG 1 cut(s) 75
ZraI GACGTC 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.