Rh7AG320200

50S ribosomal protein L21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0004346
Physical Location & Seq
Forward (+)
36249830 .. 36253795
3966 bp
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UTR
Exon/CDS
Intron
Rh7AG320200.1

Sequence Viewer

Length: 681 bp
ATGGCGTCGTCTGCAACTCTTTCCTTGCTGTGCTCCTCCTTCGCATCCCATTGCAAAATCTCACCTCCCGCCACAACTTGCCTCTCTCAATTCAACTCCAATCTCACCTTCCTCTCCCTCTCTTCTTCTTCGTCTTCGCCGCACAAGCTCTCCTCCTCTCCGCGTCCCACATTCCCTCTCCTCCCCAAGTCGTCGGAGATTGACTCCGCCGTCCTCGAAGCCGAGCTTCAAGCTCCCGAGCCTGACGACGAACCTCTTGCTTCCGAGCAGCCTGCCCCCGCCTTGGAACTTGCTGAATCCTCCGCGACGGAGCCAGCCCCAAAACGCGAGGAGATTTTCGCTGTTGTTATGATTGGAGGGCGCCAATACATTGTTATACCTGGACGATATATCTATACACAGCGCCTCAAAGGAGCCAAGGTCAATGATAAGGTTGTTCTGAACAAGGTGCTGCTTGTGGGAACAAAAACAACTACCTACATTGGAAAACCAATAGTGACAAATGCTGCTATACATGCTGTAGTTGAAGAGCAAGGACTGAATGACAAAGTGGTTGTCTTCAAGTATAAGAAGAAGAAAAATTATCGGAGAAACATTGGTCACCGACAGCCAAATTCACGCATAAGGATAACAGCTATCACAGGCTATCAAGACTATCCAGCAGTTACCCTTGAATCATAG

Protein Analysis

226

Amino Acids

24.59

Weight (kDa)

9.18

Isoelectric Point (pI)

57.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L21p PF00829 113 - 213 4.5e-30 Ribosomal prokaryotic L21 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 209
AccB1I GGYRCC 1 cut(s) 360
AccII CGCG 3 cut(s) 163, 305, 327
AciI CCGC 6 cut(s) 69, 140, 161, 207, 279, 303
AcsI RAATTY 1 cut(s) 613
AcyI GRCGYC 2 cut(s) 5, 361
AfiI CCNNNNNNNGG 1 cut(s) 283
AgsI TTSAA 5 cut(s) 94, 230, 527, 562, 674
AjnI CCWGG 1 cut(s) 379
AjuI GAANNNNNNNTTGG 2 cut(s) 92, 124
AluBI AGCT 4 cut(s) 148, 226, 233, 635
AluI AGCT 4 cut(s) 148, 226, 233, 635
Alw21I GWGCWC 1 cut(s) 35
Ama87I CYCGRG 1 cut(s) 236
ApeKI GCWGC 3 cut(s) 268, 451, 506
ApoI RAATTY 1 cut(s) 613
ArsI GACNNNNNNTTYG 2 cut(s) 540, 572
AspLEI GCGC 2 cut(s) 363, 405
AsuHPI GGTGA 3 cut(s) 54, 97, 593
AvaI CYCGRG 1 cut(s) 236
BanI GGYRCC 1 cut(s) 360
BbsI GAAGAC 2 cut(s) 126, 550
Bbv12I GWGCWC 1 cut(s) 35
BbvI GCAGC 3 cut(s) 280, 438, 493
BceAI ACGGC 1 cut(s) 194
BcgI CGANNNNNNTGC 4 cut(s) 239, 254, 273, 288
BciT130I CCWGG 1 cut(s) 381
BfmI CTRYAG 1 cut(s) 519
BfoI RGCGCY 2 cut(s) 364, 406
BisI GCNGC 4 cut(s) 140, 269, 452, 507
BlsI GCNGC 4 cut(s) 141, 270, 453, 508
Bme1390I CCNGG 1 cut(s) 381
BmeT110I CYCGRG 1 cut(s) 236
BmiI GGNNCC 3 cut(s) 312, 362, 415
BmrFI CCNGG 1 cut(s) 381
BmsI GCATC 1 cut(s) 53
BpiI GAAGAC 2 cut(s) 126, 550
BplI GAGNNNNNCTC 2 cut(s) 188, 220
BsaHI GRCGYC 2 cut(s) 5, 361
BsaJI CCNNGG 2 cut(s) 282, 417
BsaXI ACNNNNNCTCC 4 cut(s) 134, 164, 405, 435
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse3DI GCAATG 1 cut(s) 49
BseBI CCWGG 1 cut(s) 381
BseDI CCNNGG 2 cut(s) 282, 417
BseGI GGATG 1 cut(s) 44
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMI GCAATG 1 cut(s) 49
BseRI GAGGAG 5 cut(s) 25, 142, 145, 170, 344
BseXI GCAGC 3 cut(s) 280, 438, 493
Bsh1236I CGCG 3 cut(s) 163, 305, 327
BshNI GGYRCC 1 cut(s) 360
BsiHKAI GWGCWC 1 cut(s) 35
BsiHKCI CYCGRG 1 cut(s) 236
BslFI GGGAC 1 cut(s) 150
BslI CCNNNNNNNGG 1 cut(s) 283
BsmFI GGGAC 1 cut(s) 150
BsoBI CYCGRG 1 cut(s) 236
Bsp1286I GDGCHC 1 cut(s) 35
BspACI CCGC 6 cut(s) 69, 140, 161, 207, 279, 303
BspFNI CGCG 3 cut(s) 163, 305, 327
BspLI GGNNCC 3 cut(s) 312, 362, 415
BspQI GCTCTTC 1 cut(s) 522
BspT107I GGYRCC 1 cut(s) 360
BsrDI GCAATG 1 cut(s) 49
BssECI CCNNGG 2 cut(s) 282, 417
BssNI GRCGYC 2 cut(s) 5, 361
BssT1I CCWWGG 2 cut(s) 282, 417
Bst2UI CCWGG 1 cut(s) 381
Bst6I CTCTTC 2 cut(s) 127, 522
BstACI GRCGYC 2 cut(s) 5, 361
BstC8I GCNNGC 2 cut(s) 273, 315
BstEII GGTNACC 1 cut(s) 599
BstF5I GGATG 1 cut(s) 44
BstFNI CGCG 3 cut(s) 163, 305, 327
BstH2I RGCGCY 2 cut(s) 364, 406
BstHHI GCGC 2 cut(s) 363, 405
BstMWI GCNNNNNNNGC 3 cut(s) 11, 145, 515
BstNI CCWGG 1 cut(s) 381
BstNSI RCATGY 1 cut(s) 518
BstPI GGTNACC 1 cut(s) 599
BstSCI CCNGG 1 cut(s) 379
BstSFI CTRYAG 1 cut(s) 519
BstUI CGCG 3 cut(s) 163, 305, 327
BstV1I GCAGC 3 cut(s) 280, 438, 493
BstV2I GAAGAC 2 cut(s) 126, 550
BtsCI GGATG 1 cut(s) 44
Cac8I GCNNGC 2 cut(s) 273, 315
CfoI GCGC 2 cut(s) 363, 405
CseI GACGC 1 cut(s) 152
CviAII CATG 1 cut(s) 515
DinI GGCGCC 1 cut(s) 362
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
Eam1104I CTCTTC 2 cut(s) 127, 522
EarI CTCTTC 2 cut(s) 127, 522
EciI GGCGGA 1 cut(s) 196
Eco130I CCWWGG 2 cut(s) 282, 417
Eco88I CYCGRG 1 cut(s) 236
Eco91I GGTNACC 1 cut(s) 599
EcoO65I GGTNACC 1 cut(s) 599
EcoRII CCWGG 1 cut(s) 379
EcoT14I CCWWGG 2 cut(s) 282, 417
EgeI GGCGCC 1 cut(s) 362
EheI GGCGCC 1 cut(s) 362
ErhI CCWWGG 2 cut(s) 282, 417
FaeI CATG 1 cut(s) 518
FaiI YATR 9 cut(s) 350, 377, 390, 396, 512, 516, 567, 623, 679
FalI AAGNNNNNCTT 2 cut(s) 210, 242
FaqI GGGAC 1 cut(s) 150
FatI CATG 1 cut(s) 514
FauI CCCGC 2 cut(s) 76, 286
Fnu4HI GCNGC 4 cut(s) 140, 269, 452, 507
FokI GGATG 1 cut(s) 31
Fsp4HI GCNGC 4 cut(s) 140, 269, 452, 507
GlaI GCGC 2 cut(s) 362, 404
GluI GCNGC 4 cut(s) 140, 269, 452, 507
HaeII RGCGCY 2 cut(s) 364, 406
HgaI GACGC 1 cut(s) 152
HhaI GCGC 2 cut(s) 363, 405
Hin1I GRCGYC 2 cut(s) 5, 361
Hin1II CATG 1 cut(s) 518
Hin6I GCGC 2 cut(s) 361, 403
HinP1I GCGC 2 cut(s) 361, 403
HinfI GANTC 3 cut(s) 203, 296, 674
HphI GGTGA 3 cut(s) 54, 97, 593
Hpy188I TCNGA 4 cut(s) 196, 265, 441, 588
Hpy188III TCNNGA 2 cut(s) 236, 650
Hpy99I CGWCG 4 cut(s) 10, 196, 251, 310
HpyAV CCTTC 2 cut(s) 49, 118
HpyCH4V TGCA 2 cut(s) 14, 54
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 145, 515
Hsp92I GRCGYC 2 cut(s) 5, 361
Hsp92II CATG 1 cut(s) 518
HspAI GCGC 2 cut(s) 361, 403
KasI GGCGCC 1 cut(s) 360
LguI GCTCTTC 1 cut(s) 522
LmnI GCTCC 4 cut(s) 38, 238, 310, 413
LpnPI CCDG 7 cut(s) 255, 285, 327, 366, 393, 627, 672
Lsp1109I GCAGC 3 cut(s) 280, 438, 493
LweI GCATC 1 cut(s) 53
MaeIII GTNAC 3 cut(s) 496, 599, 664
MboII GAAGA 8 cut(s) 114, 117, 120, 126, 539, 550, 583, 586
MhlI GDGCHC 1 cut(s) 35
MluCI AATT 3 cut(s) 89, 580, 613
Mly113I GGCGCC 1 cut(s) 361
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 1 cut(s) 174
MspR9I CCNGG 1 cut(s) 381
MvaI CCWGG 1 cut(s) 381
MvnI CGCG 3 cut(s) 163, 305, 327
MwoI GCNNNNNNNGC 3 cut(s) 11, 145, 515
NarI GGCGCC 1 cut(s) 361
NlaIII CATG 1 cut(s) 518
NlaIV GGNNCC 3 cut(s) 312, 362, 415
NmeAIII GCCGAG 1 cut(s) 247
NmuCI GTSAC 2 cut(s) 496, 599
NspI RCATGY 1 cut(s) 518
PciSI GCTCTTC 1 cut(s) 522
PfeI GAWTC 2 cut(s) 296, 674
PkrI GCNGC 4 cut(s) 141, 270, 453, 508
PleI GAGTC 1 cut(s) 197
PluTI GGCGCC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 197
Psp6I CCWGG 1 cut(s) 379
PspEI GGTNACC 1 cut(s) 599
PspGI CCWGG 1 cut(s) 379
PspN4I GGNNCC 3 cut(s) 312, 362, 415
SapI GCTCTTC 1 cut(s) 522
SatI GCNGC 4 cut(s) 140, 269, 452, 507
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 381
SduI GDGCHC 1 cut(s) 35
SfaNI GCATC 1 cut(s) 53
SfcI CTRYAG 1 cut(s) 519
SfoI GGCGCC 1 cut(s) 362
Sse9I AATT 3 cut(s) 89, 580, 613
SsiI CCGC 6 cut(s) 69, 140, 161, 207, 279, 303
SspDI GGCGCC 1 cut(s) 360
StyD4I CCNGG 1 cut(s) 379
StyI CCWWGG 2 cut(s) 282, 417
TaqI TCGA 1 cut(s) 216
TasI AATT 3 cut(s) 89, 580, 613
TauI GCSGC 1 cut(s) 142
TfiI GAWTC 2 cut(s) 296, 674
TseFI GTSAC 2 cut(s) 496, 599
TseI GCWGC 3 cut(s) 268, 451, 506
Tsp45I GTSAC 2 cut(s) 496, 599
TspGWI ACGGA 1 cut(s) 323
XapI RAATTY 1 cut(s) 613
XceI RCATGY 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.