Rw7G026880

50S ribosomal protein L21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0004346
Physical Location & Seq
Forward (+)
34324508 .. 34328539
4032 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G026880.1

Sequence Viewer

Length: 687 bp
ATGGCGTCGTCTGCAACTCTTTCGTTGTTGTGCTCCTCCTTCGCATCCCACTGCAAAATCTCACCTCCCGCCACAACTTGCCTCCCTCAATTCAACTCCAATCTCACCTTCCTCTCCCTCTCTTCTTCTTCTTCGTCTTCACCGCACAAGCTCTCCTCCTCTCCGCGTCCCACATTCTCTCTCCTCCCCAAGTCGTCGGAGATTGACTCCGCCGTCCTCGAAGCCGAGCTTCAAGCTCCCGAGCCTGACGCCGAAACTCTTGCTTCCGAGGAGCCTGCCCCCGCCTCCGCCGCCGCCTTGGAACTTGCTGAATCCGAACCAGCCCCAAAACGCGAGGAGATTTTCGCTGTTGTTATGATTGGAGGGCGCCAGTACATTGTTATACCTGGACGATATATCTATACACAGCGCCTCAAAGGAGCCAAGGTCAATGATAAGGTTGTTCTGAACAAGGTGCTGCTTGTGGGAACAAAAACAACTACCTACATTGGAAAACCAATAGTGACAAATGCTGCTATACATGCTGTAGTTGAAGAGCAAGGACTGAATGACAAAGTGGTTGTCTTCAAGTATAAGAAGAAGAAAAATTATAGGAGAAACATTGGTCACCGACAGCCAAATTCACGCATAAGGATAACAGCTATCACCGGCTATCAAGACTATCCAGCAGTTACCCTTGAATCATAG

Protein Analysis

228

Amino Acids

24.68

Weight (kDa)

9.18

Isoelectric Point (pI)

57.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L21p PF00829 115 - 215 4.6e-30 Ribosomal prokaryotic L21 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 212
AccB1I GGYRCC 1 cut(s) 366
AccII CGCG 2 cut(s) 166, 333
AciI CCGC 8 cut(s) 69, 143, 164, 210, 282, 288, 291, 294
AcsI RAATTY 1 cut(s) 619
AcyI GRCGYC 3 cut(s) 5, 249, 367
AfaI GTAC 1 cut(s) 374
AgsI TTSAA 5 cut(s) 94, 233, 533, 568, 680
AjnI CCWGG 1 cut(s) 385
AjuI GAANNNNNNNTTGG 2 cut(s) 92, 124
AluBI AGCT 4 cut(s) 151, 229, 236, 641
AluI AGCT 4 cut(s) 151, 229, 236, 641
Alw21I GWGCWC 1 cut(s) 35
Ama87I CYCGRG 1 cut(s) 239
ApeKI GCWGC 2 cut(s) 457, 512
ApoI RAATTY 1 cut(s) 619
ArsI GACNNNNNNTTYG 2 cut(s) 546, 578
AspLEI GCGC 2 cut(s) 369, 411
AsuHPI GGTGA 5 cut(s) 54, 97, 132, 599, 637
AvaI CYCGRG 1 cut(s) 239
BanI GGYRCC 1 cut(s) 366
BbsI GAAGAC 2 cut(s) 129, 556
Bbv12I GWGCWC 1 cut(s) 35
BbvI GCAGC 2 cut(s) 444, 499
BceAI ACGGC 1 cut(s) 197
BcgI CGANNNNNNTGC 5 cut(s) 37, 242, 257, 276, 291
BciT130I CCWGG 1 cut(s) 387
BfmI CTRYAG 1 cut(s) 525
BfoI RGCGCY 2 cut(s) 370, 412
BisI GCNGC 4 cut(s) 291, 294, 458, 513
BlsI GCNGC 4 cut(s) 292, 295, 459, 514
Bme1390I CCNGG 1 cut(s) 387
BmeT110I CYCGRG 1 cut(s) 239
BmiI GGNNCC 3 cut(s) 273, 368, 421
BmrFI CCNGG 1 cut(s) 387
BmsI GCATC 1 cut(s) 53
BpiI GAAGAC 2 cut(s) 129, 556
BplI GAGNNNNNCTC 2 cut(s) 191, 223
BsaHI GRCGYC 3 cut(s) 5, 249, 367
BsaJI CCNNGG 3 cut(s) 267, 297, 423
BsaXI ACNNNNNCTCC 4 cut(s) 137, 167, 411, 441
Bse118I RCCGGY 1 cut(s) 647
Bse1I ACTGG 1 cut(s) 370
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 3 cut(s) 267, 297, 423
BseGI GGATG 1 cut(s) 44
BseNI ACTGG 1 cut(s) 370
BseRI GAGGAG 6 cut(s) 25, 145, 148, 173, 284, 350
BseXI GCAGC 2 cut(s) 444, 499
Bsh1236I CGCG 2 cut(s) 166, 333
BshNI GGYRCC 1 cut(s) 366
BsiHKAI GWGCWC 1 cut(s) 35
BsiHKCI CYCGRG 1 cut(s) 239
BsiSI CCGG 1 cut(s) 648
BslFI GGGAC 1 cut(s) 153
BsmFI GGGAC 1 cut(s) 153
BsoBI CYCGRG 1 cut(s) 239
Bsp1286I GDGCHC 1 cut(s) 35
BspACI CCGC 8 cut(s) 69, 143, 164, 210, 282, 288, 291, 294
BspFNI CGCG 2 cut(s) 166, 333
BspLI GGNNCC 3 cut(s) 273, 368, 421
BspQI GCTCTTC 1 cut(s) 528
BspT107I GGYRCC 1 cut(s) 366
BsrFI RCCGGY 1 cut(s) 647
BsrI ACTGG 1 cut(s) 370
BssAI RCCGGY 1 cut(s) 647
BssECI CCNNGG 3 cut(s) 267, 297, 423
BssNI GRCGYC 3 cut(s) 5, 249, 367
BssT1I CCWWGG 2 cut(s) 297, 423
Bst2UI CCWGG 1 cut(s) 387
Bst6I CTCTTC 2 cut(s) 127, 528
BstACI GRCGYC 3 cut(s) 5, 249, 367
BstC8I GCNNGC 1 cut(s) 276
BstEII GGTNACC 1 cut(s) 605
BstF5I GGATG 1 cut(s) 44
BstFNI CGCG 2 cut(s) 166, 333
BstH2I RGCGCY 2 cut(s) 370, 412
BstHHI GCGC 2 cut(s) 369, 411
BstMWI GCNNNNNNNGC 3 cut(s) 11, 290, 521
BstNI CCWGG 1 cut(s) 387
BstNSI RCATGY 1 cut(s) 524
BstPI GGTNACC 1 cut(s) 605
BstSCI CCNGG 1 cut(s) 385
BstSFI CTRYAG 1 cut(s) 525
BstUI CGCG 2 cut(s) 166, 333
BstV1I GCAGC 2 cut(s) 444, 499
BstV2I GAAGAC 2 cut(s) 129, 556
BtsCI GGATG 1 cut(s) 44
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 1 cut(s) 49
Cac8I GCNNGC 1 cut(s) 276
CfoI GCGC 2 cut(s) 369, 411
Cfr10I RCCGGY 1 cut(s) 647
CseI GACGC 2 cut(s) 155, 257
Csp6I GTAC 1 cut(s) 373
CviAII CATG 1 cut(s) 521
CviQI GTAC 1 cut(s) 373
DinI GGCGCC 1 cut(s) 368
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
Eam1104I CTCTTC 2 cut(s) 127, 528
EarI CTCTTC 2 cut(s) 127, 528
EciI GGCGGA 2 cut(s) 199, 277
Eco130I CCWWGG 2 cut(s) 297, 423
Eco88I CYCGRG 1 cut(s) 239
Eco91I GGTNACC 1 cut(s) 605
EcoO65I GGTNACC 1 cut(s) 605
EcoRII CCWGG 1 cut(s) 385
EcoT14I CCWWGG 2 cut(s) 297, 423
EgeI GGCGCC 1 cut(s) 368
EheI GGCGCC 1 cut(s) 368
ErhI CCWWGG 2 cut(s) 297, 423
FaeI CATG 1 cut(s) 524
FalI AAGNNNNNCTT 2 cut(s) 213, 245
FaqI GGGAC 1 cut(s) 153
FatI CATG 1 cut(s) 520
FauI CCCGC 2 cut(s) 76, 289
Fnu4HI GCNGC 4 cut(s) 291, 294, 458, 513
FokI GGATG 1 cut(s) 31
Fsp4HI GCNGC 4 cut(s) 291, 294, 458, 513
GlaI GCGC 2 cut(s) 368, 410
GluI GCNGC 4 cut(s) 291, 294, 458, 513
HaeII RGCGCY 2 cut(s) 370, 412
HapII CCGG 1 cut(s) 648
HgaI GACGC 2 cut(s) 155, 257
HhaI GCGC 2 cut(s) 369, 411
Hin1I GRCGYC 3 cut(s) 5, 249, 367
Hin1II CATG 1 cut(s) 524
Hin6I GCGC 2 cut(s) 367, 409
HinP1I GCGC 2 cut(s) 367, 409
HinfI GANTC 3 cut(s) 206, 311, 680
HpaII CCGG 1 cut(s) 648
HphI GGTGA 5 cut(s) 54, 97, 132, 599, 637
Hpy188I TCNGA 4 cut(s) 199, 268, 316, 447
Hpy188III TCNNGA 2 cut(s) 239, 656
Hpy99I CGWCG 2 cut(s) 10, 199
HpyAV CCTTC 2 cut(s) 49, 118
HpyCH4V TGCA 2 cut(s) 14, 54
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 290, 521
Hsp92I GRCGYC 3 cut(s) 5, 249, 367
Hsp92II CATG 1 cut(s) 524
HspAI GCGC 2 cut(s) 367, 409
KasI GGCGCC 1 cut(s) 366
LguI GCTCTTC 1 cut(s) 528
LmnI GCTCC 4 cut(s) 38, 241, 271, 419
LpnPI CCDG 8 cut(s) 258, 288, 333, 372, 383, 399, 661, 678
Lsp1109I GCAGC 2 cut(s) 444, 499
LweI GCATC 1 cut(s) 53
MaeIII GTNAC 3 cut(s) 502, 605, 670
MboII GAAGA 9 cut(s) 114, 117, 120, 123, 129, 545, 556, 589, 592
MhlI GDGCHC 1 cut(s) 35
MluCI AATT 3 cut(s) 89, 586, 619
Mly113I GGCGCC 1 cut(s) 367
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 177
MspI CCGG 1 cut(s) 648
MspR9I CCNGG 1 cut(s) 387
MvaI CCWGG 1 cut(s) 387
MvnI CGCG 2 cut(s) 166, 333
MwoI GCNNNNNNNGC 3 cut(s) 11, 290, 521
NarI GGCGCC 1 cut(s) 367
NlaIII CATG 1 cut(s) 524
NlaIV GGNNCC 3 cut(s) 273, 368, 421
NmeAIII GCCGAG 1 cut(s) 250
NmuCI GTSAC 2 cut(s) 502, 605
NspI RCATGY 1 cut(s) 524
PciSI GCTCTTC 1 cut(s) 528
PfeI GAWTC 2 cut(s) 311, 680
PkrI GCNGC 4 cut(s) 292, 295, 459, 514
PleI GAGTC 1 cut(s) 200
PluTI GGCGCC 1 cut(s) 370
PpsI GAGTC 1 cut(s) 200
Psp6I CCWGG 1 cut(s) 385
PspEI GGTNACC 1 cut(s) 605
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 3 cut(s) 273, 368, 421
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
SapI GCTCTTC 1 cut(s) 528
SatI GCNGC 4 cut(s) 291, 294, 458, 513
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 1 cut(s) 387
SduI GDGCHC 1 cut(s) 35
SfaNI GCATC 1 cut(s) 53
SfcI CTRYAG 1 cut(s) 525
SfoI GGCGCC 1 cut(s) 368
Sse9I AATT 3 cut(s) 89, 586, 619
SsiI CCGC 8 cut(s) 69, 143, 164, 210, 282, 288, 291, 294
SspDI GGCGCC 1 cut(s) 366
StyD4I CCNGG 1 cut(s) 385
StyI CCWWGG 2 cut(s) 297, 423
TaqI TCGA 1 cut(s) 219
TasI AATT 3 cut(s) 89, 586, 619
TatI WGTACW 1 cut(s) 372
TauI GCSGC 2 cut(s) 293, 296
TfiI GAWTC 2 cut(s) 311, 680
TscAI CASTG 1 cut(s) 56
TseFI GTSAC 2 cut(s) 502, 605
TseI GCWGC 2 cut(s) 457, 512
Tsp45I GTSAC 2 cut(s) 502, 605
TspRI CASTG 1 cut(s) 56
XapI RAATTY 1 cut(s) 619
XceI RCATGY 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.