RchiOBHm_Chr7g0218651

50S ribosomal protein L21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004346
Physical Location & Seq
Forward (+)
36808610 .. 36812800
4191 bp
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UTR
Exon/CDS
Intron
PRQ19573

Sequence Viewer

Length: 675 bp
ATGGCGTCGTCTGCAACTCTTTCGTTGTTGTGCTCCTCCTTCGCATCCCACTGCAAAATCTCACCTCCCGCCACAACTTGCCTCTCTCAATTCAACTCCAATCTCACCTTCCTCTCCCTCTCTTCTTCTTCTTCGTCTTCGCCGCACAAGCTCTCCTCCTCTCCGCGTCCCACATTCCCTCTCCTCCCCAAGTCGTCGGACATTGACTCCGCCGTCCTCGAAGCCGAGCTTCAAGCTCCCGAGCCTGACGCCGAACCTCTTGCTTCCGAGGAGCCTGCCCCCGCCTTGGAACTTGCTGAATCCGAACCAGCCCCAAAACGCGAGGAGATTTTCGCTGTTGTTTTGATTGGAGGGCGCCAGTACATTGTTATACCTGGACGATATATCTATACACAGCGCCTCAAAGGAGCCAAGGTCAATGATAAGGTTGTTCTGAACAAGGTGCTGCTTGTGGGAACAAAAACAACTACCTACATTGGAAAACCAATAGTGACAAATGCTGCTATACATGCTATAGTTGAAGAGCAAGGACTGAATGACAAAGTGGTTGTCTTCAAGTATAAGAAGAAGAAAAATTATAGGAGAAACATTGGTCACCGACAGCCAAATTCACGCATAAGGATAACAGCTATCACCGGCTATCAAGACTATCCAGCAGTTACCCTTGAATCATAG

Protein Analysis

224

Amino Acids

24.35

Weight (kDa)

9.18

Isoelectric Point (pI)

56.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L21p PF00829 111 - 211 9.9e-30 Ribosomal prokaryotic L21 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 212
AccB1I GGYRCC 1 cut(s) 354
AccII CGCG 2 cut(s) 166, 321
AciI CCGC 5 cut(s) 69, 143, 164, 210, 282
AcsI RAATTY 1 cut(s) 607
AcyI GRCGYC 3 cut(s) 5, 249, 355
AfaI GTAC 1 cut(s) 362
AfiI CCNNNNNNNGG 1 cut(s) 286
AgsI TTSAA 5 cut(s) 94, 233, 521, 556, 668
AjnI CCWGG 1 cut(s) 373
AjuI GAANNNNNNNTTGG 2 cut(s) 92, 124
AluBI AGCT 4 cut(s) 151, 229, 236, 629
AluI AGCT 4 cut(s) 151, 229, 236, 629
Alw21I GWGCWC 1 cut(s) 35
Ama87I CYCGRG 1 cut(s) 239
ApeKI GCWGC 2 cut(s) 445, 500
ApoI RAATTY 1 cut(s) 607
ArsI GACNNNNNNTTYG 2 cut(s) 534, 566
AspLEI GCGC 2 cut(s) 357, 399
AsuHPI GGTGA 4 cut(s) 54, 97, 587, 625
AvaI CYCGRG 1 cut(s) 239
BanI GGYRCC 1 cut(s) 354
BbsI GAAGAC 2 cut(s) 129, 544
Bbv12I GWGCWC 1 cut(s) 35
BbvI GCAGC 2 cut(s) 432, 487
BceAI ACGGC 1 cut(s) 197
BcgI CGANNNNNNTGC 5 cut(s) 37, 242, 257, 276, 291
BciT130I CCWGG 1 cut(s) 375
BfmI CTRYAG 1 cut(s) 513
BfoI RGCGCY 2 cut(s) 358, 400
BisI GCNGC 3 cut(s) 143, 446, 501
BlsI GCNGC 3 cut(s) 144, 447, 502
Bme1390I CCNGG 1 cut(s) 375
BmeT110I CYCGRG 1 cut(s) 239
BmiI GGNNCC 3 cut(s) 273, 356, 409
BmrFI CCNGG 1 cut(s) 375
BmsI GCATC 1 cut(s) 53
BpiI GAAGAC 2 cut(s) 129, 544
BsaHI GRCGYC 3 cut(s) 5, 249, 355
BsaJI CCNNGG 3 cut(s) 267, 285, 411
BsaXI ACNNNNNCTCC 6 cut(s) 137, 167, 191, 221, 399, 429
Bsc4I CCNNNNNNNGG 1 cut(s) 286
Bse118I RCCGGY 1 cut(s) 635
Bse1I ACTGG 1 cut(s) 358
BseBI CCWGG 1 cut(s) 375
BseDI CCNNGG 3 cut(s) 267, 285, 411
BseGI GGATG 1 cut(s) 44
BseLI CCNNNNNNNGG 1 cut(s) 286
BseNI ACTGG 1 cut(s) 358
BseRI GAGGAG 6 cut(s) 25, 145, 148, 173, 284, 338
BseXI GCAGC 2 cut(s) 432, 487
Bsh1236I CGCG 2 cut(s) 166, 321
BshNI GGYRCC 1 cut(s) 354
BsiHKAI GWGCWC 1 cut(s) 35
BsiHKCI CYCGRG 1 cut(s) 239
BsiSI CCGG 1 cut(s) 636
BslFI GGGAC 1 cut(s) 153
BslI CCNNNNNNNGG 1 cut(s) 286
BsmFI GGGAC 1 cut(s) 153
BsoBI CYCGRG 1 cut(s) 239
Bsp1286I GDGCHC 1 cut(s) 35
BspACI CCGC 5 cut(s) 69, 143, 164, 210, 282
BspFNI CGCG 2 cut(s) 166, 321
BspLI GGNNCC 3 cut(s) 273, 356, 409
BspQI GCTCTTC 1 cut(s) 516
BspT107I GGYRCC 1 cut(s) 354
BsrFI RCCGGY 1 cut(s) 635
BsrI ACTGG 1 cut(s) 358
BssAI RCCGGY 1 cut(s) 635
BssECI CCNNGG 3 cut(s) 267, 285, 411
BssNI GRCGYC 3 cut(s) 5, 249, 355
BssT1I CCWWGG 2 cut(s) 285, 411
Bst2UI CCWGG 1 cut(s) 375
Bst6I CTCTTC 2 cut(s) 127, 516
BstACI GRCGYC 3 cut(s) 5, 249, 355
BstC8I GCNNGC 1 cut(s) 276
BstEII GGTNACC 1 cut(s) 593
BstF5I GGATG 1 cut(s) 44
BstFNI CGCG 2 cut(s) 166, 321
BstH2I RGCGCY 2 cut(s) 358, 400
BstHHI GCGC 2 cut(s) 357, 399
BstMWI GCNNNNNNNGC 3 cut(s) 11, 148, 509
BstNI CCWGG 1 cut(s) 375
BstNSI RCATGY 1 cut(s) 512
BstPI GGTNACC 1 cut(s) 593
BstSCI CCNGG 1 cut(s) 373
BstSFI CTRYAG 1 cut(s) 513
BstUI CGCG 2 cut(s) 166, 321
BstV1I GCAGC 2 cut(s) 432, 487
BstV2I GAAGAC 2 cut(s) 129, 544
BtsCI GGATG 1 cut(s) 44
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 1 cut(s) 49
Cac8I GCNNGC 1 cut(s) 276
CfoI GCGC 2 cut(s) 357, 399
Cfr10I RCCGGY 1 cut(s) 635
CseI GACGC 2 cut(s) 155, 257
Csp6I GTAC 1 cut(s) 361
CviAII CATG 1 cut(s) 509
CviQI GTAC 1 cut(s) 361
DinI GGCGCC 1 cut(s) 356
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
Eam1104I CTCTTC 2 cut(s) 127, 516
EarI CTCTTC 2 cut(s) 127, 516
EciI GGCGGA 1 cut(s) 199
Eco130I CCWWGG 2 cut(s) 285, 411
Eco88I CYCGRG 1 cut(s) 239
Eco91I GGTNACC 1 cut(s) 593
EcoO65I GGTNACC 1 cut(s) 593
EcoRII CCWGG 1 cut(s) 373
EcoT14I CCWWGG 2 cut(s) 285, 411
EgeI GGCGCC 1 cut(s) 356
EheI GGCGCC 1 cut(s) 356
ErhI CCWWGG 2 cut(s) 285, 411
FaeI CATG 1 cut(s) 512
FalI AAGNNNNNCTT 2 cut(s) 213, 245
FaqI GGGAC 1 cut(s) 153
FatI CATG 1 cut(s) 508
FauI CCCGC 2 cut(s) 76, 289
Fnu4HI GCNGC 3 cut(s) 143, 446, 501
FokI GGATG 1 cut(s) 31
Fsp4HI GCNGC 3 cut(s) 143, 446, 501
GlaI GCGC 2 cut(s) 356, 398
GluI GCNGC 3 cut(s) 143, 446, 501
HaeII RGCGCY 2 cut(s) 358, 400
HapII CCGG 1 cut(s) 636
HgaI GACGC 2 cut(s) 155, 257
HhaI GCGC 2 cut(s) 357, 399
Hin1I GRCGYC 3 cut(s) 5, 249, 355
Hin1II CATG 1 cut(s) 512
Hin6I GCGC 2 cut(s) 355, 397
HinP1I GCGC 2 cut(s) 355, 397
HinfI GANTC 3 cut(s) 206, 299, 668
HpaII CCGG 1 cut(s) 636
HphI GGTGA 4 cut(s) 54, 97, 587, 625
Hpy188I TCNGA 4 cut(s) 199, 268, 304, 435
Hpy188III TCNNGA 2 cut(s) 239, 644
Hpy99I CGWCG 2 cut(s) 10, 199
HpyAV CCTTC 2 cut(s) 49, 118
HpyCH4V TGCA 2 cut(s) 14, 54
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 148, 509
Hsp92I GRCGYC 3 cut(s) 5, 249, 355
Hsp92II CATG 1 cut(s) 512
HspAI GCGC 2 cut(s) 355, 397
KasI GGCGCC 1 cut(s) 354
LguI GCTCTTC 1 cut(s) 516
LmnI GCTCC 4 cut(s) 38, 241, 271, 407
LpnPI CCDG 8 cut(s) 258, 288, 321, 360, 371, 387, 649, 666
Lsp1109I GCAGC 2 cut(s) 432, 487
LweI GCATC 1 cut(s) 53
MaeIII GTNAC 3 cut(s) 490, 593, 658
MboII GAAGA 9 cut(s) 114, 117, 120, 123, 129, 533, 544, 577, 580
MhlI GDGCHC 1 cut(s) 35
MluCI AATT 3 cut(s) 89, 574, 607
Mly113I GGCGCC 1 cut(s) 355
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 177
MspI CCGG 1 cut(s) 636
MspR9I CCNGG 1 cut(s) 375
MvaI CCWGG 1 cut(s) 375
MvnI CGCG 2 cut(s) 166, 321
MwoI GCNNNNNNNGC 3 cut(s) 11, 148, 509
NarI GGCGCC 1 cut(s) 355
NlaIII CATG 1 cut(s) 512
NlaIV GGNNCC 3 cut(s) 273, 356, 409
NmeAIII GCCGAG 1 cut(s) 250
NmuCI GTSAC 2 cut(s) 490, 593
NspI RCATGY 1 cut(s) 512
PciSI GCTCTTC 1 cut(s) 516
PfeI GAWTC 2 cut(s) 299, 668
PkrI GCNGC 3 cut(s) 144, 447, 502
PleI GAGTC 1 cut(s) 200
PluTI GGCGCC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 200
Psp6I CCWGG 1 cut(s) 373
PspEI GGTNACC 1 cut(s) 593
PspGI CCWGG 1 cut(s) 373
PspN4I GGNNCC 3 cut(s) 273, 356, 409
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
SapI GCTCTTC 1 cut(s) 516
SatI GCNGC 3 cut(s) 143, 446, 501
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 1 cut(s) 375
SduI GDGCHC 1 cut(s) 35
SfaNI GCATC 1 cut(s) 53
SfcI CTRYAG 1 cut(s) 513
SfoI GGCGCC 1 cut(s) 356
Sse9I AATT 3 cut(s) 89, 574, 607
SsiI CCGC 5 cut(s) 69, 143, 164, 210, 282
SspDI GGCGCC 1 cut(s) 354
StyD4I CCNGG 1 cut(s) 373
StyI CCWWGG 2 cut(s) 285, 411
TaqI TCGA 1 cut(s) 219
TasI AATT 3 cut(s) 89, 574, 607
TatI WGTACW 1 cut(s) 360
TauI GCSGC 1 cut(s) 145
TfiI GAWTC 2 cut(s) 299, 668
TscAI CASTG 1 cut(s) 56
TseFI GTSAC 2 cut(s) 490, 593
TseI GCWGC 2 cut(s) 445, 500
Tsp45I GTSAC 2 cut(s) 490, 593
TspRI CASTG 1 cut(s) 56
XapI RAATTY 1 cut(s) 607
XceI RCATGY 1 cut(s) 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.