AT1G61130

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003577
Physical Location & Seq
Reverse (-)
22528853 .. 22531827
2975 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G61130.1

Sequence Viewer

Length: 1392 bp
ATGATGAACATTTCCAATGTGTCCATTGCTTTGTATCTTTGCACTCTTTTCGCATTTGTTTCATCTGATAGTCCAGAAGCAATGAGAGATCTTGTGACAAACTTTCCTGGCCAACCAAAAGTGAGCTTCAGACATTACGCCGGTTATGTCACGGTTAATATAATCAGTGGGAGGGCTTTATTTTATTGGTTCTTCGAAGCTATGACTCATCCTAACGTGAAGCCTTTAGTATTGTGGCTTAATGGAGGTCCTGGATGTTCTTCTGTGGGATACGGGGCTACACAAGAGATTGGTCCATTTCTTGTCGATAATAAAGGAAACAGTCTTAAGTTTAACCCCTATGCATGGAATAAAGAGGCCAATATTCTCTTTCTTGAATCTCCTGCTGGTGTTGGGTTTTCGTATTCAAACACAAGTAGTGATTATAGAAAACTTGGCGATGACTTTACAGCGAGAGACTCATACACTTTTCTCCAAAAGTGGTTCTTGAGATTCCCAGCTTACAAAGAAAAGGACTTCTTCATCGCAGGAGAGAGCTATGCAGGAAAATACGTACCCGAGCTTGCAGAAGTTATTTATGACAAGAACAAGGACAACGAAAACTTGTCTCTTCACATTAATTTAAAAGGCATTTTGCTTGGAAATCCATTGACATCATATGCGGAAGATTGGACAGGATGGGTGGATTATGCGTGGAATCACGCAGTGGTATCAGACGAGACATACAGAGTCATAAAACAAAGTTGCAATTTCAGTAGTGACACTACATGGGACGTTAAAGATTGCAAAGAAGGCGTGGACGAAATACTCAAACAATACAAAGAGATCGATCAATTCAGCCTGTACACTCCTATCTGCATGCACCATTCATCAAAGGTTGATTCTTATGCAAATTACAAGACGACGATACCGAGGCTGTTCGATGGATTTGACCCGTGTTTGGATGATTACGCAAAAGTATTCTACAATAGAGCTGATGTTCAGAAGGCTCTTCACGCGACTGATGGTGTTCATCTCAAGAACTGGACCATTTGCAACGATGATATTTTAAATCACTGGAACTGGACTGATTCAAAGCGATCCGTTTTGCCTATATATAAGAAACTCATTGCCGGAGGATTTAGAGTTTGGGTTTATAGTGGCGATACTGATGGAAGAGTACCGGTACTCTCGACAAGGTACTGCATAAATAAACTGGAATTACCAATCAAGACAGCTTGGAGGCCATGGTACCATGAGACACAGGTAAGTGGATGGTTTCAGGAATATGAAGGTCTAACGTTTGCAACGTTCAGAGGAGCAGGACATGATGTGCCTTCCTTCAAACCTAGCGAATCCCTTGCATTTTTCTCTGCCTTTCTCAACGGAGTTCCTCCTCCTTTGTCGCGATAG

Protein Analysis

463

Amino Acids

52.82

Weight (kDa)

6.02

Isoelectric Point (pI)

39.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 36 - 456 1.7e-144 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1230
AccB1I GGYRCC 1 cut(s) 1230
AccII CGCG 2 cut(s) 998, 1387
AciI CCGC 1 cut(s) 662
AclI AACGTT 2 cut(s) 1280, 1289
AclWI GGATC 1 cut(s) 1074
AcoI YGGCCR 1 cut(s) 109
AcuI CTGAAG 1 cut(s) 112
AdeI CACNNNGTG 1 cut(s) 706
AfaI GTAC 6 cut(s) 555, 845, 1161, 1167, 1181, 1232
AfiI CCNNNNNNNGG 2 cut(s) 345, 940
AflII CTTAAG 1 cut(s) 326
AgeI ACCGGT 1 cut(s) 1162
AgsI TTSAA 4 cut(s) 377, 408, 1074, 1324
AjnI CCWGG 2 cut(s) 106, 250
AluBI AGCT 7 cut(s) 126, 200, 500, 537, 562, 974, 1217
AluI AGCT 7 cut(s) 126, 200, 500, 537, 562, 974, 1217
Alw26I GTCTC 4 cut(s) 450, 612, 713, 1232
AlwI GGATC 1 cut(s) 1074
Ama87I CYCGRG 1 cut(s) 557
AoxI GGCC 3 cut(s) 109, 357, 1223
AseI ATTAAT 1 cut(s) 618
AsiGI ACCGGT 1 cut(s) 1162
Asp718I GGTACC 1 cut(s) 1230
AspS9I GGNCC 3 cut(s) 248, 293, 1026
AsuII TTCGAA 1 cut(s) 195
AvaI CYCGRG 1 cut(s) 557
AvaII GGWCC 3 cut(s) 248, 293, 1026
BalI TGGCCA 1 cut(s) 111
BanI GGYRCC 1 cut(s) 1230
BccI CCATC 5 cut(s) 672, 917, 998, 1145, 1248
BcgI CGANNNNNNTGC 4 cut(s) 31, 65, 1322, 1356
BciT130I CCWGG 2 cut(s) 108, 252
BciVI GTATCC 1 cut(s) 263
BcoDI GTCTC 4 cut(s) 450, 612, 713, 1232
BfaI CTAG 1 cut(s) 1329
BfrI CTTAAG 1 cut(s) 326
BfuI GTATCC 1 cut(s) 263
BglII AGATCT 1 cut(s) 88
Bme1390I CCNGG 2 cut(s) 108, 252
Bme18I GGWCC 3 cut(s) 248, 293, 1026
BmeT110I CYCGRG 1 cut(s) 557
BmgT120I GGNCC 3 cut(s) 248, 293, 1026
BmiI GGNNCC 1 cut(s) 1232
BmrFI CCNGG 2 cut(s) 108, 252
Bpu14I TTCGAA 1 cut(s) 195
BpuEI CTTGAG 2 cut(s) 508, 1001
Bsa29I ATCGAT 1 cut(s) 828
BsaAI YACGTR 1 cut(s) 553
BsaJI CCNNGG 2 cut(s) 911, 1226
BsaWI WCCGGW 1 cut(s) 1162
Bsc4I CCNNNNNNNGG 2 cut(s) 345, 940
Bse118I RCCGGY 2 cut(s) 140, 1162
Bse1I ACTGG 4 cut(s) 1028, 1061, 1067, 1200
Bse3DI GCAATG 3 cut(s) 24, 87, 1107
BseBI CCWGG 2 cut(s) 108, 252
BseCI ATCGAT 1 cut(s) 828
BseDI CCNNGG 2 cut(s) 911, 1226
BseGI GGATG 5 cut(s) 208, 260, 683, 949, 1259
BseLI CCNNNNNNNGG 2 cut(s) 345, 940
BseMI GCAATG 3 cut(s) 24, 87, 1107
BseNI ACTGG 4 cut(s) 1028, 1061, 1067, 1200
BseRI GAGGAG 2 cut(s) 1311, 1365
BseYI CCCAGC 1 cut(s) 496
Bsh1236I CGCG 2 cut(s) 998, 1387
BshFI GGCC 3 cut(s) 111, 359, 1225
BshNI GGYRCC 1 cut(s) 1230
BshTI ACCGGT 1 cut(s) 1162
BshVI ATCGAT 1 cut(s) 828
BsiHKCI CYCGRG 1 cut(s) 557
BsiSI CCGG 3 cut(s) 141, 1113, 1163
BslFI GGGAC 1 cut(s) 785
BslI CCNNNNNNNGG 2 cut(s) 345, 940
BsmAI GTCTC 4 cut(s) 450, 612, 713, 1232
BsmFI GGGAC 1 cut(s) 785
BsnI GGCC 3 cut(s) 111, 359, 1225
BsoBI CYCGRG 1 cut(s) 557
Bsp119I TTCGAA 1 cut(s) 195
Bsp1407I TGTACA 1 cut(s) 843
Bsp143I GATC 4 cut(s) 88, 825, 829, 1079
Bsp19I CCATGG 1 cut(s) 1226
Bsp68I TCGCGA 1 cut(s) 1387
BspACI CCGC 1 cut(s) 662
BspANI GGCC 3 cut(s) 111, 359, 1225
BspDI ATCGAT 1 cut(s) 828
BspFNI CGCG 2 cut(s) 998, 1387
BspLI GGNNCC 1 cut(s) 1232
BspPI GGATC 1 cut(s) 1074
BspQI GCTCTTC 1 cut(s) 996
BspT104I TTCGAA 1 cut(s) 195
BspT107I GGYRCC 1 cut(s) 1230
BspTI CTTAAG 1 cut(s) 326
BsrDI GCAATG 3 cut(s) 24, 87, 1107
BsrFI RCCGGY 2 cut(s) 140, 1162
BsrGI TGTACA 1 cut(s) 843
BsrI ACTGG 4 cut(s) 1028, 1061, 1067, 1200
BssAI RCCGGY 2 cut(s) 140, 1162
BssECI CCNNGG 2 cut(s) 911, 1226
BssMI GATC 4 cut(s) 88, 825, 829, 1079
BssT1I CCWWGG 1 cut(s) 1226
Bst2UI CCWGG 2 cut(s) 108, 252
Bst4CI ACNGT 2 cut(s) 154, 323
Bst6I CTCTTC 3 cut(s) 615, 996, 1150
BstAFI CTTAAG 1 cut(s) 326
BstAUI TGTACA 1 cut(s) 843
BstBAI YACGTR 1 cut(s) 553
BstBI TTCGAA 1 cut(s) 195
BstC8I GCNNGC 2 cut(s) 564, 860
BstDSI CCRYGG 1 cut(s) 1226
BstF5I GGATG 5 cut(s) 208, 260, 683, 949, 1259
BstFNI CGCG 2 cut(s) 998, 1387
BstKTI GATC 4 cut(s) 91, 828, 832, 1082
BstMAI GTCTC 4 cut(s) 450, 612, 713, 1232
BstMBI GATC 4 cut(s) 88, 825, 829, 1079
BstMWI GCNNNNNNNGC 2 cut(s) 792, 995
BstNI CCWGG 2 cut(s) 108, 252
BstNSI RCATGY 1 cut(s) 862
BstSCI CCNGG 2 cut(s) 106, 250
BstSNI TACGTA 1 cut(s) 553
BstUI CGCG 2 cut(s) 998, 1387
BstX2I RGATCY 1 cut(s) 88
BstYI RGATCY 1 cut(s) 88
Bsu15I ATCGAT 1 cut(s) 828
BsuI GTATCC 1 cut(s) 263
BsuRI GGCC 3 cut(s) 111, 359, 1225
BsuTUI ATCGAT 1 cut(s) 828
BtgI CCRYGG 1 cut(s) 1226
BtgZI GCGATG 2 cut(s) 453, 508
BtsCI GGATG 5 cut(s) 208, 260, 683, 949, 1259
BtsI GCAGTG 1 cut(s) 711
BtsIMutI CAGTG 3 cut(s) 172, 711, 1054
BtuMI TCGCGA 1 cut(s) 1387
Cac8I GCNNGC 2 cut(s) 564, 860
Cfr10I RCCGGY 2 cut(s) 140, 1162
Cfr13I GGNCC 3 cut(s) 248, 293, 1026
ClaI ATCGAT 1 cut(s) 828
Csp6I GTAC 6 cut(s) 554, 844, 1160, 1166, 1180, 1231
CspAI ACCGGT 1 cut(s) 1162
CviAII CATG 6 cut(s) 345, 768, 859, 1227, 1235, 1307
CviQI GTAC 6 cut(s) 554, 844, 1160, 1166, 1180, 1231
DpnI GATC 4 cut(s) 90, 827, 831, 1081
DpnII GATC 4 cut(s) 88, 825, 829, 1079
DraI TTTAAA 2 cut(s) 624, 1050
DraIII CACNNNGTG 1 cut(s) 706
EaeI YGGCCR 1 cut(s) 109
Eam1104I CTCTTC 3 cut(s) 615, 996, 1150
EarI CTCTTC 3 cut(s) 615, 996, 1150
Eco105I TACGTA 1 cut(s) 553
Eco130I CCWWGG 1 cut(s) 1226
Eco47I GGWCC 3 cut(s) 248, 293, 1026
Eco57I CTGAAG 1 cut(s) 112
Eco88I CYCGRG 1 cut(s) 557
EcoO109I RGGNCCY 1 cut(s) 248
EcoRII CCWGG 2 cut(s) 106, 250
EcoT14I CCWWGG 1 cut(s) 1226
EcoT22I ATGCAT 1 cut(s) 346
ErhI CCWWGG 1 cut(s) 1226
FaeI CATG 6 cut(s) 348, 771, 862, 1230, 1238, 1310
FalI AAGNNNNNCTT 4 cut(s) 470, 502, 503, 535
FaqI GGGAC 1 cut(s) 785
FatI CATG 6 cut(s) 344, 767, 858, 1226, 1234, 1306
FauNDI CATATG 1 cut(s) 658
FokI GGATG 5 cut(s) 195, 267, 690, 956, 1266
FspBI CTAG 1 cut(s) 1329
GsaI CCCAGC 1 cut(s) 500
HaeIII GGCC 3 cut(s) 111, 359, 1225
HapII CCGG 3 cut(s) 141, 1113, 1163
Hin1II CATG 6 cut(s) 348, 771, 862, 1230, 1238, 1310
HinfI GANTC 9 cut(s) 205, 377, 458, 492, 697, 729, 881, 1070, 1334
HpaII CCGG 3 cut(s) 141, 1113, 1163
Hpy166II GTNNAC 2 cut(s) 799, 846
Hpy188I TCNGA 5 cut(s) 67, 131, 715, 984, 1295
Hpy188III TCNNGA 8 cut(s) 74, 374, 487, 1018, 1171, 1210, 1262, 1386
Hpy8I GTNNAC 2 cut(s) 799, 846
Hpy99I CGWCG 1 cut(s) 907
HpyAV CCTTC 5 cut(s) 785, 979, 1265, 1326, 1330
HpyCH4III ACNGT 2 cut(s) 154, 323
HpyCH4IV ACGT 5 cut(s) 216, 552, 774, 1280, 1289
HpyF10VI GCNNNNNNNGC 2 cut(s) 792, 995
HpySE526I ACGT 5 cut(s) 216, 552, 774, 1280, 1289
Hsp92II CATG 6 cut(s) 348, 771, 862, 1230, 1238, 1310
KpnI GGTACC 1 cut(s) 1234
Kzo9I GATC 4 cut(s) 88, 825, 829, 1079
LguI GCTCTTC 1 cut(s) 996
LmnI GCTCC 1 cut(s) 1298
MaeI CTAG 1 cut(s) 1329
MaeII ACGT 5 cut(s) 216, 552, 774, 1280, 1289
MaeIII GTNAC 3 cut(s) 94, 148, 758
MalI GATC 4 cut(s) 90, 827, 831, 1081
MboI GATC 4 cut(s) 88, 825, 829, 1079
MboII GAAGA 7 cut(s) 184, 252, 511, 602, 677, 983, 1167
MflI RGATCY 1 cut(s) 88
MlsI TGGCCA 1 cut(s) 111
MluCI AATT 5 cut(s) 619, 748, 833, 892, 1199
MluNI TGGCCA 1 cut(s) 111
MlyI GAGTC 3 cut(s) 199, 452, 738
MnlI CCTC 9 cut(s) 165, 239, 349, 906, 1109, 1215, 1289, 1383, 1386
Mox20I TGGCCA 1 cut(s) 111
Mph1103I ATGCAT 1 cut(s) 346
MscI TGGCCA 1 cut(s) 111
MseI TTAA 8 cut(s) 156, 240, 327, 333, 618, 623, 777, 1049
Msp20I TGGCCA 1 cut(s) 111
MspCI CTTAAG 1 cut(s) 326
MspI CCGG 3 cut(s) 141, 1113, 1163
MspR9I CCNGG 2 cut(s) 108, 252
MvaI CCWGG 2 cut(s) 108, 252
MvnI CGCG 2 cut(s) 998, 1387
MwoI GCNNNNNNNGC 2 cut(s) 792, 995
NcoI CCATGG 1 cut(s) 1226
NdeI CATATG 1 cut(s) 658
NdeII GATC 4 cut(s) 88, 825, 829, 1079
NlaIII CATG 6 cut(s) 348, 771, 862, 1230, 1238, 1310
NlaIV GGNNCC 1 cut(s) 1232
NmuCI GTSAC 3 cut(s) 94, 148, 758
NruI TCGCGA 1 cut(s) 1387
NsiI ATGCAT 1 cut(s) 346
NspI RCATGY 1 cut(s) 862
NspV TTCGAA 1 cut(s) 195
PaeI GCATGC 1 cut(s) 862
PciSI GCTCTTC 1 cut(s) 996
PcsI WCGNNNNNNNCGW 2 cut(s) 908, 1286
PfeI GAWTC 6 cut(s) 377, 492, 697, 881, 1070, 1334
PfoI TCCNGGA 1 cut(s) 250
PinAI ACCGGT 1 cut(s) 1162
PleI GAGTC 3 cut(s) 199, 452, 737
PpsI GAGTC 3 cut(s) 199, 452, 737
Ppu21I YACGTR 1 cut(s) 553
PpuMI RGGWCCY 1 cut(s) 248
PshBI ATTAAT 1 cut(s) 618
Psp1406I AACGTT 2 cut(s) 1280, 1289
Psp5II RGGWCCY 1 cut(s) 248
Psp6I CCWGG 2 cut(s) 106, 250
PspFI CCCAGC 1 cut(s) 496
PspGI CCWGG 2 cut(s) 106, 250
PspN4I GGNNCC 1 cut(s) 1232
PspPI GGNCC 3 cut(s) 248, 293, 1026
PspPPI RGGWCCY 1 cut(s) 248
PsuI RGATCY 1 cut(s) 88
RruI TCGCGA 1 cut(s) 1387
RsaI GTAC 6 cut(s) 555, 845, 1161, 1167, 1181, 1232
RsaNI GTAC 6 cut(s) 554, 844, 1160, 1166, 1180, 1231
SapI GCTCTTC 1 cut(s) 996
SaqAI TTAA 8 cut(s) 156, 240, 327, 333, 618, 623, 777, 1049
Sau3AI GATC 4 cut(s) 88, 825, 829, 1079
Sau96I GGNCC 3 cut(s) 248, 293, 1026
SchI GAGTC 3 cut(s) 199, 452, 738
ScrFI CCNGG 2 cut(s) 108, 252
SfuI TTCGAA 1 cut(s) 195
SinI GGWCC 3 cut(s) 248, 293, 1026
SmlI CTYRAG 3 cut(s) 326, 487, 1016
SmoI CTYRAG 3 cut(s) 326, 487, 1016
SnaBI TACGTA 1 cut(s) 553
SphI GCATGC 1 cut(s) 862
Sse9I AATT 5 cut(s) 619, 748, 833, 892, 1199
SsiI CCGC 1 cut(s) 662
SspI AATATT 1 cut(s) 364
SspMI CTAG 1 cut(s) 1329
StyD4I CCNGG 2 cut(s) 106, 250
StyI CCWWGG 1 cut(s) 1226
TaaI ACNGT 2 cut(s) 154, 323
TaiI ACGT 5 cut(s) 219, 555, 777, 1283, 1292
TaqI TCGA 5 cut(s) 195, 306, 828, 921, 1172
TasI AATT 5 cut(s) 619, 748, 833, 892, 1199
TatI WGTACW 1 cut(s) 843
TfiI GAWTC 6 cut(s) 377, 492, 697, 881, 1070, 1334
Tru1I TTAA 8 cut(s) 156, 240, 327, 333, 618, 623, 777, 1049
Tru9I TTAA 8 cut(s) 156, 240, 327, 333, 618, 623, 777, 1049
TscAI CASTG 3 cut(s) 172, 711, 1061
TseFI GTSAC 3 cut(s) 94, 148, 758
Tsp45I GTSAC 3 cut(s) 94, 148, 758
TspDTI ATGAA 6 cut(s) 20, 51, 511, 858, 1001, 1284
TspGWI ACGGA 2 cut(s) 1072, 1380
TspRI CASTG 3 cut(s) 172, 711, 1061
Vha464I CTTAAG 1 cut(s) 326
VpaK11BI GGWCC 3 cut(s) 248, 293, 1026
VspI ATTAAT 1 cut(s) 618
XceI RCATGY 1 cut(s) 862
XspI CTAG 1 cut(s) 1329
Zsp2I ATGCAT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.