Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003577

Physical Location & Seq

Genomic Coordinates Reverse (-)

44937689 .. 44941780

Gene Structure 4092 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1322000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1443 bp

ATGAATATTGTATCAAAAGCGACGGCTTTCTTTGTTTTGGCTCTCGTTGCACTGCTGTTTGTGGAGCCTGTTTCGGGTAGAACAAAAAGGGCTAGTACTGAGAAACTGAACAACAACTCACTGGGGACTCAGGACCTTGTGACCAACTTGCCCGGCCAGCCTGCTGCAGACTTCAAGCACTACGCTGGCTATGTGACCGTGAACGAAACAAATGGGAGGGCTCTTTTTTACTGGTTTTATGAGGCTGCCTCCAACCCAGATGGAAAGCCTCTTGTGTTGTGGCTTAATGGAGGTCCTGGGTGCTCTTCTGTGGGATATGGAGCAACCCAAGAGATTGGTCCATTCATAGTGGACACTGATGGAAGTGGAATTAAATCTAATAACTACTCATGGAATAAAGAGGCCAATATGTTATTCTTGGAATCTCCAGTTGGGGTTGGCTTTTCATATTCTAATACAACTAGGGACTATGATAATCTCGGAGATGAGTTTACAGCGAATGATGCGTATAACTTCTTGCATAAGTGGTATCTCAAGTTCCCAACATACAGAACAAGGACCTTCTACATTTCTGGGGAGAGTTATGCAGGAAAGTACGTTCCAGAGCTCGCCGAGCTCATATATGACAAGAACAATAATCCTTCCCTTCATATTGATATCAAGGGTATCCTGCTGGGTAATCCTGAAACATCTGATGCTGATGACTGGAGAGGTCTGGTAGATTATGCTTGGAGTCATGCTGTGATATCAGACGAAACTCACAAAATAATACGCGAAAGCTGTGATTTCGACAGAAATGATACTTGGAGTAACAAGGATTGTACTCTAGCTGTGGATGAAGTGCTAAAACAGTACAAGGAGATAGATATTTACAGCCTTTACACCTCGGTCTGCATTGGTGATACGGCAAGCTCAACTGATGGTTCGATGCAGGTCATGATGAAACGCACATCTACGATGATGCCAAGGATTATGGGTGGCTATGATCCATGTCTCGATGGATATTCAAAAACCTTTTATAACAGACCAGATGTGCAAAAGGCCCTCCACGTTAGTGATGGTGTCCGGCTCAAGAACTGGACCATCTGCAACAAAAAAATATTTGAGGACTGGGCATATTCGAAGCCTTCTGTTCTTCCTATATACAAGAAACTCATTGCAGCAGGACTTAGAATATGGGTGTACAGCGGAGACACAGACGGAAGAGTTCCAGTGCTTTCCACAAGATACAGCTTAAGTGCTCTGGGATTGCCTATTACTAAGGCATGGAGGCCTTGGTACCACGAGAAGCAGGTTAGTGGATGGTTTCAAGAATATGAAGGTCTTACATTTGCAACATTTAGAGGAGCTGGGCATGCAGTTCCCTGCTTCAAACCAAGCAACTCACTGGCATTGTTCACGTCTTTTCTCCTCGGCGATCCTCTCCCAACCGCTCGAGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004180 GO:0004185 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0009056 GO:0009057 GO:0009987 GO:0016787 GO:0017171 GO:0019538 GO:0030163 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0070008 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S10

Protein Analysis

481

Amino Acids

53.78

Weight (kDa)

5.96

Isoelectric Point (pI)

28.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S10	PF00450	51 - 471	1.6e-141	Serine carboxypeptidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003577)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11080 AT1G11080 AT1G61130 AT4G15100
fragaria_vesca	FvH4_3g04600 FvH4_3g04600 FvH4_3g04600 FvH4_3g04620
malus_domestica	MD05G1322000.v1.1 MD10G1300000.v1.1
prunus_persica	Prupe.4G040800_v2.0.a1 Prupe.4G040800_v2.0.a1
pyrus_communis	pycom05g29630 pycom10g25270
rosa_chinensis	RchiOBHm_Chr5g0007541 RchiOBHm_Chr5g0007551
rosa_laevigata	RLG00000031466 RLG00000031467
rosa_multiflora	Rmu_ssc0000182.1_g000013
rosa_roxburghii	Rroxscaffold_1G00068870 Rroxscaffold_1G00068880
rosa_rugosa	Rorug04G0428200 Rorug04G0428300 Rorug04G0428400 Rorug04G0428500
rosa_samantha	Rh5AG055900 Rh5AG056100 Rh5BG053600 Rh5BG053700 Rh5CG063400 Rh5CG063500 Rh5DG052600 Rh5DG052700
rosa_wichuraiana	Rw5G005400 Rw5G005410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1020
Acc36I	ACCTGC	2 cut(s)	922, 1282
Acc65I	GGTACC	1 cut(s)	1278
AccB1I	GGYRCC	1 cut(s)	1278
AccBSI	CCGCTC	1 cut(s)	1433
AccII	CGCG	1 cut(s)	774
AciI	CCGC	2 cut(s)	1188, 1431
AclWI	GGATC	2 cut(s)	980, 1412
AcoI	YGGCCR	1 cut(s)	154
AfaI	GTAC	6 cut(s)	97, 596, 823, 854, 1184, 1280
AfiI	CCNNNNNNNGG	1 cut(s)	74
AflII	CTTAAG	1 cut(s)	1234
AgsI	TTSAA	4 cut(s)	175, 1008, 1310, 1372
AjiI	CACGTC	1 cut(s)	1401
AjnI	CCWGG	1 cut(s)	295
AjuI	GAANNNNNNNTTGG	6 cut(s)	398, 414, 430, 446, 787, 819
AleI	CACNNNNGTG	1 cut(s)	1053
AloI	GAACNNNNNNTCC	2 cut(s)	582, 614
AluBI	AGCT	7 cut(s)	607, 616, 780, 830, 912, 1233, 1349
AluI	AGCT	7 cut(s)	607, 616, 780, 830, 912, 1233, 1349
Alw21I	GWGCWC	4 cut(s)	305, 609, 618, 1243
Alw26I	GTCTC	2 cut(s)	998, 1185
AlwI	GGATC	2 cut(s)	980, 1412
Ama87I	CYCGRG	1 cut(s)	1434
AoxI	GGCC	4 cut(s)	154, 402, 1041, 1271
ApeKI	GCWGC	3 cut(s)	164, 245, 1160
Asp718I	GGTACC	1 cut(s)	1278
AspS9I	GGNCC	6 cut(s)	133, 293, 338, 558, 1042, 1080
AsuC2I	CCSGG	1 cut(s)	153
AsuHPI	GGTGA	1 cut(s)	911
AsuII	TTCGAA	1 cut(s)	1121
AvaI	CYCGRG	1 cut(s)	1434
AvaII	GGWCC	5 cut(s)	133, 293, 338, 558, 1080
BanI	GGYRCC	1 cut(s)	1278
BanII	GRGCYC	3 cut(s)	223, 609, 618
BauI	CACGAG	1 cut(s)	1283
Bbv12I	GWGCWC	4 cut(s)	305, 609, 618, 1243
BbvI	GCAGC	3 cut(s)	151, 232, 1172
BccI	CCATC	7 cut(s)	254, 353, 914, 992, 1052, 1091, 1296
BceAI	ACGGC	2 cut(s)	39, 921
BciT130I	CCWGG	1 cut(s)	297
BciVI	GTATCC	1 cut(s)	677
BcnI	CCSGG	1 cut(s)	153
BcoDI	GTCTC	2 cut(s)	998, 1185
BfaI	CTAG	3 cut(s)	93, 462, 827
BfmI	CTRYAG	1 cut(s)	165
BfrI	CTTAAG	1 cut(s)	1234
BfuAI	ACCTGC	2 cut(s)	922, 1282
BfuI	GTATCC	1 cut(s)	677
BisI	GCNGC	3 cut(s)	165, 246, 1161
BlsI	GCNGC	3 cut(s)	166, 247, 1162
BmcAI	AGTACT	1 cut(s)	97
Bme1390I	CCNGG	2 cut(s)	153, 297
Bme18I	GGWCC	5 cut(s)	133, 293, 338, 558, 1080
BmeT110I	CYCGRG	1 cut(s)	1434
BmgBI	CACGTC	1 cut(s)	1401
BmgT120I	GGNCC	6 cut(s)	133, 293, 338, 558, 1042, 1080
BmiI	GGNNCC	2 cut(s)	66, 1280
BmrFI	CCNGG	2 cut(s)	153, 297
BmrI	ACTGGG	2 cut(s)	131, 1120
BmsI	GCATC	4 cut(s)	493, 685, 918, 951
BmuI	ACTGGG	2 cut(s)	131, 1120
BplI	GAGNNNNNCTC	2 cut(s)	233, 265
BpmI	CTGGAG	2 cut(s)	411, 727
Bpu14I	TTCGAA	1 cut(s)	1121
BpuEI	CTTGAG	2 cut(s)	518, 1055
BpuMI	CCSGG	1 cut(s)	153
BsaJI	CCNNGG	5 cut(s)	296, 885, 965, 1274, 1411
Bsc4I	CCNNNNNNNGG	1 cut(s)	74
Bse1I	ACTGG	8 cut(s)	126, 236, 428, 710, 1082, 1115, 1211, 1392
Bse3DI	GCAATG	1 cut(s)	1155
BseBI	CCWGG	1 cut(s)	297
BseDI	CCNNGG	5 cut(s)	296, 885, 965, 1274, 1411
BseGI	GGATG	2 cut(s)	841, 1307
BseLI	CCNNNNNNNGG	1 cut(s)	74
BseMI	GCAATG	1 cut(s)	1155
BseMII	CTCAG	2 cut(s)	90, 143
BseNI	ACTGG	8 cut(s)	126, 236, 428, 710, 1082, 1115, 1211, 1392
BseRI	GAGGAG	2 cut(s)	1359, 1400
BseXI	GCAGC	3 cut(s)	151, 232, 1172
BseYI	CCCAGC	2 cut(s)	673, 1349
Bsh1236I	CGCG	1 cut(s)	774
BshFI	GGCC	4 cut(s)	156, 404, 1043, 1273
BshNI	GGYRCC	1 cut(s)	1278
BsiHKAI	GWGCWC	4 cut(s)	305, 609, 618, 1243
BsiHKCI	CYCGRG	1 cut(s)	1434
BsiSI	CCGG	2 cut(s)	153, 1066
BslFI	GGGAC	2 cut(s)	139, 479
BslI	CCNNNNNNNGG	1 cut(s)	74
BsmAI	GTCTC	2 cut(s)	998, 1185
BsmFI	GGGAC	2 cut(s)	139, 479
BsnI	GGCC	4 cut(s)	156, 404, 1043, 1273
BsoBI	CYCGRG	1 cut(s)	1434
Bsp119I	TTCGAA	1 cut(s)	1121
Bsp1286I	GDGCHC	5 cut(s)	223, 305, 609, 618, 1243
Bsp1407I	TGTACA	1 cut(s)	1182
Bsp143I	GATC	2 cut(s)	985, 1417
BspACI	CCGC	2 cut(s)	1188, 1431
BspANI	GGCC	4 cut(s)	156, 404, 1043, 1273
BspCNI	CTCAG	2 cut(s)	91, 142
BspFNI	CGCG	1 cut(s)	774
BspHI	TCATGA	1 cut(s)	936
BspLI	GGNNCC	2 cut(s)	66, 1280
BspMAI	CTGCAG	1 cut(s)	169
BspMI	ACCTGC	2 cut(s)	922, 1282
BspPI	GGATC	2 cut(s)	980, 1412
BspQI	GCTCTTC	1 cut(s)	310
BspT104I	TTCGAA	1 cut(s)	1121
BspT107I	GGYRCC	1 cut(s)	1278
BspTI	CTTAAG	1 cut(s)	1234
BsrBI	CCGCTC	1 cut(s)	1433
BsrDI	GCAATG	1 cut(s)	1155
BsrGI	TGTACA	1 cut(s)	1182
BsrI	ACTGG	8 cut(s)	126, 236, 428, 710, 1082, 1115, 1211, 1392
BssECI	CCNNGG	5 cut(s)	296, 885, 965, 1274, 1411
BssMI	GATC	2 cut(s)	985, 1417
BssSI	CACGAG	1 cut(s)	1283
BssT1I	CCWWGG	2 cut(s)	965, 1274
Bst2BI	CACGAG	1 cut(s)	1283
Bst2UI	CCWGG	1 cut(s)	297
Bst4CI	ACNGT	2 cut(s)	199, 852
Bst6I	CTCTTC	2 cut(s)	310, 1198
BstAFI	CTTAAG	1 cut(s)	1234
BstAUI	TGTACA	1 cut(s)	1182
BstBI	TTCGAA	1 cut(s)	1121
BstC8I	GCNNGC	6 cut(s)	158, 162, 187, 609, 910, 1356
BstDEI	CTNAG	4 cut(s)	99, 129, 1169, 1260
BstF5I	GGATG	2 cut(s)	841, 1307
BstFNI	CGCG	1 cut(s)	774
BstKTI	GATC	2 cut(s)	988, 1420
BstMAI	GTCTC	2 cut(s)	998, 1185
BstMBI	GATC	2 cut(s)	985, 1417
BstMWI	GCNNNNNNNGC	5 cut(s)	47, 157, 503, 613, 1355
BstNI	CCWGG	1 cut(s)	297
BstNSI	RCATGY	1 cut(s)	1358
BstSCI	CCNGG	2 cut(s)	151, 295
BstSFI	CTRYAG	1 cut(s)	165
BstUI	CGCG	1 cut(s)	774
BstV1I	GCAGC	3 cut(s)	151, 232, 1172
BstXI	CCANNNNNNTGG	1 cut(s)	335
BsuI	GTATCC	1 cut(s)	677
BsuRI	GGCC	4 cut(s)	156, 404, 1043, 1273
BtrI	CACGTC	1 cut(s)	1401
BtsCI	GGATG	2 cut(s)	841, 1307
BtsI	GCAGTG	1 cut(s)	50
BtsIMutI	CAGTG	5 cut(s)	50, 119, 354, 1218, 1385
BveI	ACCTGC	2 cut(s)	922, 1282
Cac8I	GCNNGC	6 cut(s)	158, 162, 187, 609, 910, 1356
CciI	TCATGA	1 cut(s)	936
Cfr13I	GGNCC	6 cut(s)	133, 293, 338, 558, 1042, 1080
Csp6I	GTAC	6 cut(s)	96, 595, 822, 853, 1183, 1279
CviAII	CATG	6 cut(s)	390, 737, 937, 990, 1266, 1355
CviQI	GTAC	6 cut(s)	96, 595, 822, 853, 1183, 1279
DdeI	CTNAG	4 cut(s)	99, 129, 1169, 1260
DpnI	GATC	2 cut(s)	987, 1419
DpnII	GATC	2 cut(s)	985, 1417
EaeI	YGGCCR	1 cut(s)	154
Eam1104I	CTCTTC	2 cut(s)	310, 1198
EarI	CTCTTC	2 cut(s)	310, 1198
Ecl136II	GAGCTC	2 cut(s)	607, 616
Eco130I	CCWWGG	2 cut(s)	965, 1274
Eco147I	AGGCCT	1 cut(s)	1273
Eco24I	GRGCYC	3 cut(s)	223, 609, 618
Eco32I	GATATC	2 cut(s)	658, 747
Eco47I	GGWCC	5 cut(s)	133, 293, 338, 558, 1080
Eco53kI	GAGCTC	2 cut(s)	607, 616
Eco88I	CYCGRG	1 cut(s)	1434
EcoICRI	GAGCTC	2 cut(s)	607, 616
EcoO109I	RGGNCCY	4 cut(s)	133, 293, 558, 1042
EcoRII	CCWGG	1 cut(s)	295
EcoRV	GATATC	2 cut(s)	658, 747
EcoT14I	CCWWGG	2 cut(s)	965, 1274
EcoT38I	GRGCYC	3 cut(s)	223, 609, 618
ErhI	CCWWGG	2 cut(s)	965, 1274
FaeI	CATG	6 cut(s)	393, 740, 940, 993, 1269, 1358
FaqI	GGGAC	2 cut(s)	139, 479
FatI	CATG	6 cut(s)	389, 736, 936, 989, 1265, 1354
Fnu4HI	GCNGC	3 cut(s)	165, 246, 1161
FokI	GGATG	2 cut(s)	848, 1314
FriOI	GRGCYC	3 cut(s)	223, 609, 618
Fsp4HI	GCNGC	3 cut(s)	165, 246, 1161
FspBI	CTAG	3 cut(s)	93, 462, 827
GluI	GCNGC	3 cut(s)	165, 246, 1161
GsaI	CCCAGC	2 cut(s)	677, 1353
GsuI	CTGGAG	2 cut(s)	411, 727
HaeIII	GGCC	4 cut(s)	156, 404, 1043, 1273
HapII	CCGG	2 cut(s)	153, 1066
Hin1II	CATG	6 cut(s)	393, 740, 940, 993, 1269, 1358
HinfI	GANTC	3 cut(s)	127, 422, 733
HpaII	CCGG	2 cut(s)	153, 1066
HphI	GGTGA	1 cut(s)	911
Hpy166II	GTNNAC	5 cut(s)	202, 352, 492, 1183, 1398
Hpy188I	TCNGA	3 cut(s)	482, 694, 751
Hpy188III	TCNNGA	7 cut(s)	131, 602, 683, 937, 995, 1072, 1310
Hpy8I	GTNNAC	5 cut(s)	202, 352, 492, 1183, 1398
Hpy99I	CGWCG	1 cut(s)	25
HpyAV	CCTTC	5 cut(s)	571, 651, 656, 1137, 1313
HpyCH4III	ACNGT	2 cut(s)	199, 852
HpyCH4IV	ACGT	3 cut(s)	597, 1050, 1400
HpyF10VI	GCNNNNNNNGC	5 cut(s)	47, 157, 503, 613, 1355
HpyF3I	CTNAG	4 cut(s)	99, 129, 1169, 1260
HpySE526I	ACGT	3 cut(s)	597, 1050, 1400
Hsp92II	CATG	6 cut(s)	393, 740, 940, 993, 1269, 1358
KpnI	GGTACC	1 cut(s)	1282
Kzo9I	GATC	2 cut(s)	985, 1417
LguI	GCTCTTC	1 cut(s)	310
LmnI	GCTCC	3 cut(s)	64, 320, 1346
Lsp1109I	GCAGC	3 cut(s)	151, 232, 1172
LweI	GCATC	4 cut(s)	493, 685, 918, 951
MaeI	CTAG	3 cut(s)	93, 462, 827
MaeII	ACGT	3 cut(s)	597, 1050, 1400
MaeIII	GTNAC	3 cut(s)	139, 193, 809
MalI	GATC	2 cut(s)	987, 1419
MbiI	CCGCTC	1 cut(s)	1433
MboI	GATC	2 cut(s)	985, 1417
MboII	GAAGA	3 cut(s)	297, 1127, 1215
MhlI	GDGCHC	5 cut(s)	223, 305, 609, 618, 1243
MluCI	AATT	1 cut(s)	369
MlyI	GAGTC	2 cut(s)	121, 742
MmeI	TCCRAC	1 cut(s)	276
MseI	TTAA	3 cut(s)	285, 372, 1235
MslI	CAYNNNNRTG	1 cut(s)	1053
MspA1I	CMGCKG	1 cut(s)	1188
MspCI	CTTAAG	1 cut(s)	1234
MspI	CCGG	2 cut(s)	153, 1066
MspR9I	CCNGG	2 cut(s)	153, 297
MvaI	CCWGG	1 cut(s)	297
MvnI	CGCG	1 cut(s)	774
MwoI	GCNNNNNNNGC	5 cut(s)	47, 157, 503, 613, 1355
NciI	CCSGG	1 cut(s)	153
NdeII	GATC	2 cut(s)	985, 1417
NlaIII	CATG	6 cut(s)	393, 740, 940, 993, 1269, 1358
NlaIV	GGNNCC	2 cut(s)	66, 1280
NmeAIII	GCCGAG	2 cut(s)	637, 1392
NmuCI	GTSAC	2 cut(s)	139, 193
NspI	RCATGY	1 cut(s)	1358
NspV	TTCGAA	1 cut(s)	1121
OliI	CACNNNNGTG	1 cut(s)	1053
PaeI	GCATGC	1 cut(s)	1358
PaeR7I	CTCGAG	1 cut(s)	1434
PagI	TCATGA	1 cut(s)	936
PceI	AGGCCT	1 cut(s)	1273
PciSI	GCTCTTC	1 cut(s)	310
PfeI	GAWTC	1 cut(s)	422
PkrI	GCNGC	3 cut(s)	166, 247, 1162
PleI	GAGTC	2 cut(s)	121, 741
PpsI	GAGTC	2 cut(s)	121, 741
PpuMI	RGGWCCY	3 cut(s)	133, 293, 558
PsiI	TTATAA	1 cut(s)	1020
Psp124BI	GAGCTC	2 cut(s)	609, 618
Psp5II	RGGWCCY	3 cut(s)	133, 293, 558
Psp6I	CCWGG	1 cut(s)	295
PspFI	CCCAGC	2 cut(s)	673, 1349
PspGI	CCWGG	1 cut(s)	295
PspN4I	GGNNCC	2 cut(s)	66, 1280
PspPI	GGNCC	6 cut(s)	133, 293, 338, 558, 1042, 1080
PspPPI	RGGWCCY	3 cut(s)	133, 293, 558
PspXI	VCTCGAGB	1 cut(s)	1434
PsrI	GAACNNNNNNTAC	2 cut(s)	521, 553
PstI	CTGCAG	1 cut(s)	169
RsaI	GTAC	6 cut(s)	97, 596, 823, 854, 1184, 1280
RsaNI	GTAC	6 cut(s)	96, 595, 822, 853, 1183, 1279
RseI	CAYNNNNRTG	1 cut(s)	1053
SacI	GAGCTC	2 cut(s)	609, 618
SapI	GCTCTTC	1 cut(s)	310
SaqAI	TTAA	3 cut(s)	285, 372, 1235
SatI	GCNGC	3 cut(s)	165, 246, 1161
Sau3AI	GATC	2 cut(s)	985, 1417
Sau96I	GGNCC	6 cut(s)	133, 293, 338, 558, 1042, 1080
ScaI	AGTACT	1 cut(s)	97
SchI	GAGTC	2 cut(s)	121, 742
ScrFI	CCNGG	2 cut(s)	153, 297
SduI	GDGCHC	5 cut(s)	223, 305, 609, 618, 1243
SfaNI	GCATC	4 cut(s)	493, 685, 918, 951
SfcI	CTRYAG	1 cut(s)	165
Sfr274I	CTCGAG	1 cut(s)	1434
SfuI	TTCGAA	1 cut(s)	1121
SinI	GGWCC	5 cut(s)	133, 293, 338, 558, 1080
SlaI	CTCGAG	1 cut(s)	1434
SmiMI	CAYNNNNRTG	1 cut(s)	1053
SmlI	CTYRAG	4 cut(s)	533, 1070, 1234, 1434
SmoI	CTYRAG	4 cut(s)	533, 1070, 1234, 1434
SphI	GCATGC	1 cut(s)	1358
Sse9I	AATT	1 cut(s)	369
SseBI	AGGCCT	1 cut(s)	1273
SsiI	CCGC	2 cut(s)	1188, 1431
SspI	AATATT	2 cut(s)	7, 1101
SspMI	CTAG	3 cut(s)	93, 462, 827
SstI	GAGCTC	2 cut(s)	609, 618
StuI	AGGCCT	1 cut(s)	1273
StyD4I	CCNGG	2 cut(s)	151, 295
StyI	CCWWGG	2 cut(s)	965, 1274
TaaI	ACNGT	2 cut(s)	199, 852
TaiI	ACGT	3 cut(s)	600, 1053, 1403
TaqI	TCGA	5 cut(s)	789, 926, 996, 1121, 1435
TaqII	GACCGA	1 cut(s)	877
TasI	AATT	1 cut(s)	369
TatI	WGTACW	4 cut(s)	95, 821, 852, 1182
TfiI	GAWTC	1 cut(s)	422
Tru1I	TTAA	3 cut(s)	285, 372, 1235
Tru9I	TTAA	3 cut(s)	285, 372, 1235
TscAI	CASTG	5 cut(s)	57, 126, 361, 1218, 1392
TseFI	GTSAC	2 cut(s)	139, 193
TseI	GCWGC	3 cut(s)	164, 245, 1160
Tsp45I	GTSAC	2 cut(s)	139, 193
TspDTI	ATGAA	7 cut(s)	17, 334, 435, 638, 852, 956, 1332
TspGWI	ACGGA	1 cut(s)	1215
TspRI	CASTG	5 cut(s)	57, 126, 361, 1218, 1392
Vha464I	CTTAAG	1 cut(s)	1234
VpaK11BI	GGWCC	5 cut(s)	133, 293, 338, 558, 1080
XceI	RCATGY	1 cut(s)	1358
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1055
XhoI	CTCGAG	1 cut(s)	1434
XspI	CTAG	3 cut(s)	93, 462, 827
ZrmI	AGTACT	1 cut(s)	97

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.