Rw5G005410

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003577
Physical Location & Seq
Reverse (-)
5675884 .. 5679024
3141 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G005410.1

Sequence Viewer

Length: 1443 bp
ATGAATTTTTTAGTAGTAAAAGCAACAGCTATCTTCCTAATTTTGGCTCTTACTGTGGTCGAGCCAAAGCCTGTTCCGGATGCTAGAAAGAAGCGGTCTAATACTGAGAAATTGATCAACTTATTGGCGAGTCAGGATCTTGTGACCAACCTGCCTGGCCAGCCTGCTGTAGACTTCCGGCACTATGCTGGCTATGTCACTGTCAATGAACCAAATGGCAGGGCTCTCTTTTACTGGTTTTATGAGGCTGCTACTAACCCAGATCTAAAACCTCTTGTTTTGTGGCTTAATGGAGGTCCTGGGTGCTCTTCTGTGGGATATGGAGCAACCCAAGAGATTGGTCCATTCATAGTGGAAACTGATGGCCATGGACTTAGATTTAATAACTACTCATGGAATAAAGAGGCCAACATGCTGTTCTTGGAATCTCCAGTTGGGGTTGGCTTTTCATACTCCAATACAACTACTGATTATGATAATCTCGGAGATGCCTTTACAGCAAATGATGCATATGCGTTTCTGCACAAGTGGTTTCTCAAGTTCCCATCTTACAGAACAAGAACCTTCTACATTGCAGGGGAGAGTTATGCAGGAAAATATGTTCCGGAGCTGGCCGAGCTCATCAATGACAAGAATACAGATCCTTCCCTCCAAATTGATCTCAAGGGAGTCCTGCTGGGTAATCCTGAAACATCAGATGCTGAGGATTGGAGAGGTATAGTGGATTATGCTTGGAGTCATGCTGTGATATCAGACGAAACTCACAAAGTTATAAGCGAAAACTGTGATTTTGACGGTGATACTTGGAGTAATAAGATCTGTACTCAAGCTGTGGATGAAGTACTAAAACAATACAAGGAGATAGACATTTACAGCCTTTATACCTCAGTCTGCATCGGTGATACAGCAAGTTCAAATGATGGTTCGATGCAAGTAATGATGATGAAGCACACATCTACAATGATGCCAAGAATTATGGGTGGCTATGATCCGTGTCTGGATGATTATTCAAGAAAATTCTATAACAGAGCAGATGTGCAAAAGGCCCTCCATGTTAGTAATGGTCCCCAGCTCAAGAACTGGAGCATCTGCAGTACCAAAATATTTCATGAGTGGGTTGATTCAAAGCCATCTGTTCTTCCTATATACAAGAAACTCATTGCAGCAGGAATGAGAATATGGGTGTACAGTGGAGACACGGATGGAAGAGTTCCTGTGCTGTCCACGAGATATAGCTTAAGTGCTCTGGGACTGCCTATTACAAAAGCATGGACACCGTGGTACCACCAAAAGCAGGTGAGTGGGTGGTTTCAAGAATATAAGGGCCTTACATTTGCAACATTCAGAGGAGCCGGCCATGCAGTTCCTTGCTTCAAACCAAGCAACTCACTGGCTTTATTCACCTCTTTTCTCCTTGGGGAATCTCTGCCTTCTGCTCGATGA

Protein Analysis

480

Amino Acids

53.74

Weight (kDa)

5.96

Isoelectric Point (pI)

28.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 52 - 472 4.5e-141 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 773
AarI CACCTGC 1 cut(s) 1285
Acc36I ACCTGC 2 cut(s) 159, 1285
Acc65I GGTACC 1 cut(s) 1281
AccB1I GGYRCC 1 cut(s) 1281
AccI GTMKAC 1 cut(s) 171
AccIII TCCGGA 2 cut(s) 76, 604
AciI CCGC 1 cut(s) 94
AclWI GGATC 3 cut(s) 144, 635, 983
AcoI YGGCCR 4 cut(s) 157, 364, 612, 1354
AcsI RAATTY 2 cut(s) 4, 1016
AfaI GTAC 5 cut(s) 823, 843, 1096, 1187, 1283
AfiI CCNNNNNNNGG 2 cut(s) 43, 1294
AflII CTTAAG 1 cut(s) 1237
AgsI TTSAA 5 cut(s) 915, 1011, 1125, 1313, 1375
AjnI CCWGG 2 cut(s) 154, 298
AjuI GAANNNNNNNTTGG 4 cut(s) 401, 417, 433, 449
AloI GAACNNNNNNTCC 2 cut(s) 585, 617
AluBI AGCT 6 cut(s) 29, 610, 619, 830, 1072, 1236
AluI AGCT 6 cut(s) 29, 610, 619, 830, 1072, 1236
Alw21I GWGCWC 3 cut(s) 308, 621, 1246
Alw26I GTCTC 1 cut(s) 1188
AlwI GGATC 3 cut(s) 144, 635, 983
AlwNI CAGNNNCTG 1 cut(s) 701
Aor13HI TCCGGA 2 cut(s) 76, 604
AoxI GGCC 7 cut(s) 157, 364, 405, 612, 1044, 1324, 1354
ApeKI GCWGC 2 cut(s) 248, 1163
ApoI RAATTY 2 cut(s) 4, 1016
Asp718I GGTACC 1 cut(s) 1281
AspS9I GGNCC 5 cut(s) 296, 341, 1045, 1064, 1324
AsuHPI GGTGA 4 cut(s) 809, 911, 1309, 1393
AvaII GGWCC 3 cut(s) 296, 341, 1064
BaeI ACNNNNGTAYC 2 cut(s) 1265, 1298
BalI TGGCCA 2 cut(s) 159, 366
BanI GGYRCC 1 cut(s) 1281
BanII GRGCYC 2 cut(s) 226, 621
BauI CACGAG 1 cut(s) 1225
Bbv12I GWGCWC 3 cut(s) 308, 621, 1246
BbvCI CCTCAGC 1 cut(s) 702
BbvI GCAGC 2 cut(s) 235, 1175
BccI CCATC 5 cut(s) 356, 553, 914, 1138, 1196
BciT130I CCWGG 2 cut(s) 156, 300
BclI TGATCA 1 cut(s) 114
BcoDI GTCTC 1 cut(s) 1188
BfaI CTAG 1 cut(s) 84
BfmI CTRYAG 2 cut(s) 168, 1090
BfrI CTTAAG 1 cut(s) 1237
BfuAI ACCTGC 2 cut(s) 159, 1285
BglII AGATCT 2 cut(s) 262, 816
BisI GCNGC 2 cut(s) 249, 1164
BlsI GCNGC 2 cut(s) 250, 1165
BmcAI AGTACT 1 cut(s) 843
Bme1390I CCNGG 2 cut(s) 156, 300
Bme18I GGWCC 3 cut(s) 296, 341, 1064
BmgT120I GGNCC 5 cut(s) 296, 341, 1045, 1064, 1324
BmiI GGNNCC 3 cut(s) 1066, 1283, 1351
BmrFI CCNGG 2 cut(s) 156, 300
BmsI GCATC 8 cut(s) 70, 478, 496, 688, 903, 918, 954, 1095
BpmI CTGGAG 2 cut(s) 414, 1102
Bpu10I CCTNAGC 1 cut(s) 702
BpuEI CTTGAG 4 cut(s) 521, 647, 810, 1058
BsaJI CCNNGG 4 cut(s) 299, 367, 1277, 1414
BsaWI WCCGGW 2 cut(s) 76, 604
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 1294
Bse118I RCCGGY 1 cut(s) 1352
Bse1I ACTGG 4 cut(s) 239, 431, 1085, 1395
Bse3DI GCAATG 2 cut(s) 570, 1158
BseAI TCCGGA 2 cut(s) 76, 604
BseBI CCWGG 2 cut(s) 156, 300
BseDI CCNNGG 4 cut(s) 299, 367, 1277, 1414
BseGI GGATG 4 cut(s) 85, 841, 1006, 1207
BseLI CCNNNNNNNGG 2 cut(s) 43, 1294
BseMI GCAATG 2 cut(s) 570, 1158
BseMII CTCAG 3 cut(s) 96, 693, 900
BseNI ACTGG 4 cut(s) 239, 431, 1085, 1395
BseRI GAGGAG 1 cut(s) 1362
BseXI GCAGC 2 cut(s) 235, 1175
BseYI CCCAGC 2 cut(s) 676, 1068
BsgI GTGCAG 1 cut(s) 506
BshFI GGCC 7 cut(s) 159, 366, 407, 614, 1046, 1326, 1356
BshNI GGYRCC 1 cut(s) 1281
BsiHKAI GWGCWC 3 cut(s) 308, 621, 1246
BsiSI CCGG 4 cut(s) 77, 178, 605, 1353
BslFI GGGAC 2 cut(s) 1050, 1263
BslI CCNNNNNNNGG 2 cut(s) 43, 1294
BsmAI GTCTC 1 cut(s) 1188
BsmFI GGGAC 2 cut(s) 1050, 1263
BsnI GGCC 7 cut(s) 159, 366, 407, 614, 1046, 1326, 1356
Bsp1286I GDGCHC 4 cut(s) 226, 308, 621, 1246
Bsp13I TCCGGA 2 cut(s) 76, 604
Bsp1407I TGTACA 1 cut(s) 1185
Bsp143I GATC 7 cut(s) 114, 136, 262, 640, 658, 816, 988
Bsp19I CCATGG 1 cut(s) 367
BspACI CCGC 1 cut(s) 94
BspANI GGCC 7 cut(s) 159, 366, 407, 614, 1046, 1326, 1356
BspCNI CTCAG 3 cut(s) 97, 694, 899
BspEI TCCGGA 2 cut(s) 76, 604
BspHI TCATGA 1 cut(s) 1108
BspLI GGNNCC 3 cut(s) 1066, 1283, 1351
BspMAI CTGCAG 1 cut(s) 1094
BspMI ACCTGC 2 cut(s) 159, 1285
BspPI GGATC 3 cut(s) 144, 635, 983
BspQI GCTCTTC 1 cut(s) 313
BspT107I GGYRCC 1 cut(s) 1281
BspTI CTTAAG 1 cut(s) 1237
BsrDI GCAATG 2 cut(s) 570, 1158
BsrFI RCCGGY 1 cut(s) 1352
BsrGI TGTACA 1 cut(s) 1185
BsrI ACTGG 4 cut(s) 239, 431, 1085, 1395
BssAI RCCGGY 1 cut(s) 1352
BssECI CCNNGG 4 cut(s) 299, 367, 1277, 1414
BssMI GATC 7 cut(s) 114, 136, 262, 640, 658, 816, 988
BssSI CACGAG 1 cut(s) 1225
BssT1I CCWWGG 2 cut(s) 367, 1414
Bst2BI CACGAG 1 cut(s) 1225
Bst2UI CCWGG 2 cut(s) 156, 300
Bst4CI ACNGT 6 cut(s) 55, 202, 785, 797, 1190, 1278
Bst6I CTCTTC 2 cut(s) 313, 1201
BstAFI CTTAAG 1 cut(s) 1237
BstAPI GCANNNNNTGC 1 cut(s) 506
BstAUI TGTACA 1 cut(s) 1185
BstC8I GCNNGC 5 cut(s) 161, 165, 190, 612, 1354
BstDEI CTNAG 4 cut(s) 105, 374, 702, 886
BstDSI CCRYGG 2 cut(s) 367, 1277
BstF5I GGATG 4 cut(s) 85, 841, 1006, 1207
BstKTI GATC 7 cut(s) 117, 139, 265, 643, 661, 819, 991
BstMAI GTCTC 1 cut(s) 1188
BstMBI GATC 7 cut(s) 114, 136, 262, 640, 658, 816, 988
BstMWI GCNNNNNNNGC 5 cut(s) 160, 497, 506, 616, 1358
BstNI CCWGG 2 cut(s) 156, 300
BstNSI RCATGY 1 cut(s) 415
BstSCI CCNGG 2 cut(s) 154, 298
BstSFI CTRYAG 2 cut(s) 168, 1090
BstV1I GCAGC 2 cut(s) 235, 1175
BstX2I RGATCY 4 cut(s) 136, 262, 640, 816
BstXI CCANNNNNNTGG 1 cut(s) 338
BstYI RGATCY 4 cut(s) 136, 262, 640, 816
BsuRI GGCC 7 cut(s) 159, 366, 407, 614, 1046, 1326, 1356
BtgI CCRYGG 2 cut(s) 367, 1277
BtsCI GGATG 4 cut(s) 85, 841, 1006, 1207
BtsIMutI CAGTG 3 cut(s) 198, 1195, 1388
BveI ACCTGC 2 cut(s) 159, 1285
Cac8I GCNNGC 5 cut(s) 161, 165, 190, 612, 1354
CaiI CAGNNNCTG 1 cut(s) 701
CciI TCATGA 1 cut(s) 1108
Cfr10I RCCGGY 1 cut(s) 1352
Cfr13I GGNCC 5 cut(s) 296, 341, 1045, 1064, 1324
Csp6I GTAC 5 cut(s) 822, 842, 1095, 1186, 1282
CviAII CATG 8 cut(s) 368, 393, 412, 740, 1052, 1109, 1269, 1358
CviQI GTAC 5 cut(s) 822, 842, 1095, 1186, 1282
DdeI CTNAG 4 cut(s) 105, 374, 702, 886
DpnI GATC 7 cut(s) 116, 138, 264, 642, 660, 818, 990
DpnII GATC 7 cut(s) 114, 136, 262, 640, 658, 816, 988
EaeI YGGCCR 4 cut(s) 157, 364, 612, 1354
Eam1104I CTCTTC 2 cut(s) 313, 1201
EarI CTCTTC 2 cut(s) 313, 1201
Ecl136II GAGCTC 1 cut(s) 619
Eco130I CCWWGG 2 cut(s) 367, 1414
Eco24I GRGCYC 2 cut(s) 226, 621
Eco32I GATATC 1 cut(s) 750
Eco47I GGWCC 3 cut(s) 296, 341, 1064
Eco53kI GAGCTC 1 cut(s) 619
EcoICRI GAGCTC 1 cut(s) 619
EcoO109I RGGNCCY 3 cut(s) 296, 1045, 1324
EcoRII CCWGG 2 cut(s) 154, 298
EcoRV GATATC 1 cut(s) 750
EcoT14I CCWWGG 2 cut(s) 367, 1414
EcoT22I ATGCAT 1 cut(s) 511
EcoT38I GRGCYC 2 cut(s) 226, 621
ErhI CCWWGG 2 cut(s) 367, 1414
FaeI CATG 8 cut(s) 371, 396, 415, 743, 1055, 1112, 1272, 1361
FaqI GGGAC 2 cut(s) 1050, 1263
FatI CATG 8 cut(s) 367, 392, 411, 739, 1051, 1108, 1268, 1357
FauNDI CATATG 1 cut(s) 511
FbaI TGATCA 1 cut(s) 114
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 2 cut(s) 249, 1164
FokI GGATG 4 cut(s) 92, 848, 1013, 1214
FriOI GRGCYC 2 cut(s) 226, 621
Fsp4HI GCNGC 2 cut(s) 249, 1164
FspBI CTAG 1 cut(s) 84
GluI GCNGC 2 cut(s) 249, 1164
GsaI CCCAGC 2 cut(s) 680, 1072
GsuI CTGGAG 2 cut(s) 414, 1102
HaeIII GGCC 7 cut(s) 159, 366, 407, 614, 1046, 1326, 1356
HapII CCGG 4 cut(s) 77, 178, 605, 1353
Hin1II CATG 8 cut(s) 371, 396, 415, 743, 1055, 1112, 1272, 1361
HinfI GANTC 6 cut(s) 130, 425, 669, 736, 1121, 1421
HpaII CCGG 4 cut(s) 77, 178, 605, 1353
HphI GGTGA 4 cut(s) 809, 911, 1309, 1393
Hpy166II GTNNAC 3 cut(s) 172, 1186, 1224
Hpy188I TCNGA 4 cut(s) 485, 697, 754, 1346
Hpy188III TCNNGA 9 cut(s) 77, 134, 605, 686, 998, 1011, 1075, 1109, 1313
Hpy8I GTNNAC 3 cut(s) 172, 1186, 1224
HpyAV CCTTC 3 cut(s) 574, 654, 1440
HpyCH4III ACNGT 6 cut(s) 55, 202, 785, 797, 1190, 1278
HpyF10VI GCNNNNNNNGC 5 cut(s) 160, 497, 506, 616, 1358
HpyF3I CTNAG 4 cut(s) 105, 374, 702, 886
Hsp92II CATG 8 cut(s) 371, 396, 415, 743, 1055, 1112, 1272, 1361
Kpn2I TCCGGA 2 cut(s) 76, 604
KpnI GGTACC 1 cut(s) 1285
KroI GCCGGC 1 cut(s) 1352
KroNI GCCGGC 1 cut(s) 1354
Ksp22I TGATCA 1 cut(s) 114
Kzo9I GATC 7 cut(s) 114, 136, 262, 640, 658, 816, 988
LguI GCTCTTC 1 cut(s) 313
LmnI GCTCC 4 cut(s) 323, 607, 1083, 1349
Lsp1109I GCAGC 2 cut(s) 235, 1175
LweI GCATC 8 cut(s) 70, 478, 496, 688, 903, 918, 954, 1095
MaeI CTAG 1 cut(s) 84
MaeIII GTNAC 2 cut(s) 142, 196
MalI GATC 7 cut(s) 116, 138, 264, 642, 660, 818, 990
MboI GATC 7 cut(s) 114, 136, 262, 640, 658, 816, 988
MboII GAAGA 4 cut(s) 25, 300, 1130, 1218
MflI RGATCY 4 cut(s) 136, 262, 640, 816
MhlI GDGCHC 4 cut(s) 226, 308, 621, 1246
MlsI TGGCCA 2 cut(s) 159, 366
MluCI AATT 6 cut(s) 4, 39, 110, 654, 972, 1016
MluNI TGGCCA 2 cut(s) 159, 366
MlyI GAGTC 3 cut(s) 139, 678, 745
Mox20I TGGCCA 2 cut(s) 159, 366
Mph1103I ATGCAT 1 cut(s) 511
MroI TCCGGA 2 cut(s) 76, 604
MroNI GCCGGC 1 cut(s) 1352
MscI TGGCCA 2 cut(s) 159, 366
MseI TTAA 3 cut(s) 288, 381, 1238
Msp20I TGGCCA 2 cut(s) 159, 366
MspCI CTTAAG 1 cut(s) 1237
MspI CCGG 4 cut(s) 77, 178, 605, 1353
MspR9I CCNGG 2 cut(s) 156, 300
MvaI CCWGG 2 cut(s) 156, 300
MwoI GCNNNNNNNGC 5 cut(s) 160, 497, 506, 616, 1358
NaeI GCCGGC 1 cut(s) 1354
NcoI CCATGG 1 cut(s) 367
NdeI CATATG 1 cut(s) 511
NdeII GATC 7 cut(s) 114, 136, 262, 640, 658, 816, 988
NgoMIV GCCGGC 1 cut(s) 1352
NlaIII CATG 8 cut(s) 371, 396, 415, 743, 1055, 1112, 1272, 1361
NlaIV GGNNCC 3 cut(s) 1066, 1283, 1351
NmeAIII GCCGAG 1 cut(s) 640
NmuCI GTSAC 2 cut(s) 142, 196
NsiI ATGCAT 1 cut(s) 511
NspI RCATGY 1 cut(s) 415
PagI TCATGA 1 cut(s) 1108
PaqCI CACCTGC 1 cut(s) 1285
PciSI GCTCTTC 1 cut(s) 313
PdiI GCCGGC 1 cut(s) 1354
PfeI GAWTC 3 cut(s) 425, 1121, 1421
PkrI GCNGC 2 cut(s) 250, 1165
PleI GAGTC 3 cut(s) 138, 677, 744
PpsI GAGTC 3 cut(s) 138, 677, 744
PpuMI RGGWCCY 1 cut(s) 296
PsiI TTATAA 1 cut(s) 773
Psp124BI GAGCTC 1 cut(s) 621
Psp5II RGGWCCY 1 cut(s) 296
Psp6I CCWGG 2 cut(s) 154, 298
PspFI CCCAGC 2 cut(s) 676, 1068
PspGI CCWGG 2 cut(s) 154, 298
PspN4I GGNNCC 3 cut(s) 1066, 1283, 1351
PspPI GGNCC 5 cut(s) 296, 341, 1045, 1064, 1324
PspPPI RGGWCCY 1 cut(s) 296
PstI CTGCAG 1 cut(s) 1094
PstNI CAGNNNCTG 1 cut(s) 701
PsuI RGATCY 4 cut(s) 136, 262, 640, 816
RsaI GTAC 5 cut(s) 823, 843, 1096, 1187, 1283
RsaNI GTAC 5 cut(s) 822, 842, 1095, 1186, 1282
SacI GAGCTC 1 cut(s) 621
SapI GCTCTTC 1 cut(s) 313
SaqAI TTAA 3 cut(s) 288, 381, 1238
SatI GCNGC 2 cut(s) 249, 1164
Sau3AI GATC 7 cut(s) 114, 136, 262, 640, 658, 816, 988
Sau96I GGNCC 5 cut(s) 296, 341, 1045, 1064, 1324
ScaI AGTACT 1 cut(s) 843
SchI GAGTC 3 cut(s) 139, 678, 745
ScrFI CCNGG 2 cut(s) 156, 300
SduI GDGCHC 4 cut(s) 226, 308, 621, 1246
SfaNI GCATC 8 cut(s) 70, 478, 496, 688, 903, 918, 954, 1095
SfcI CTRYAG 2 cut(s) 168, 1090
SinI GGWCC 3 cut(s) 296, 341, 1064
SmlI CTYRAG 5 cut(s) 536, 662, 825, 1073, 1237
SmoI CTYRAG 5 cut(s) 536, 662, 825, 1073, 1237
Sse9I AATT 6 cut(s) 4, 39, 110, 654, 972, 1016
SsiI CCGC 1 cut(s) 94
SspI AATATT 1 cut(s) 1104
SspMI CTAG 1 cut(s) 84
SstI GAGCTC 1 cut(s) 621
StyD4I CCNGG 2 cut(s) 154, 298
StyI CCWWGG 2 cut(s) 367, 1414
TaaI ACNGT 6 cut(s) 55, 202, 785, 797, 1190, 1278
TaqI TCGA 3 cut(s) 60, 926, 1438
TasI AATT 6 cut(s) 4, 39, 110, 654, 972, 1016
TatI WGTACW 3 cut(s) 821, 841, 1185
TfiI GAWTC 3 cut(s) 425, 1121, 1421
Tru1I TTAA 3 cut(s) 288, 381, 1238
Tru9I TTAA 3 cut(s) 288, 381, 1238
TscAI CASTG 3 cut(s) 205, 1195, 1395
TseFI GTSAC 2 cut(s) 142, 196
TseI GCWGC 2 cut(s) 248, 1163
Tsp45I GTSAC 2 cut(s) 142, 196
TspDTI ATGAA 7 cut(s) 17, 222, 337, 438, 852, 959, 1097
TspGWI ACGGA 2 cut(s) 981, 1214
TspRI CASTG 3 cut(s) 205, 1195, 1395
Vha464I CTTAAG 1 cut(s) 1237
VpaK11BI GGWCC 3 cut(s) 296, 341, 1064
XapI RAATTY 2 cut(s) 4, 1016
XceI RCATGY 1 cut(s) 415
XcmI CCANNNNNNNNNTGG 1 cut(s) 1058
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 1 cut(s) 84
ZrmI AGTACT 1 cut(s) 843
Zsp2I ATGCAT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.