RchiOBHm_Chr5g0007541

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003577
Physical Location & Seq
Forward (+)
4754494 .. 4757636
3143 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28858

Sequence Viewer

Length: 1413 bp
ATGAATTTTGTAGTAGTAAAAGCAACAGTTATCTTCCTAATTTTGGCTCTTACTGTGGTCGAGCCAAAGCCTGTTCCGGATGCTAGAAAGAAGCGGTCTAATACTGAGAAATTGATCAACTCATTGGCGAGTCAGGATCTTGTGACCAACCTGCCTGGCCAGCCTGCTGTAGACTTCCGGCACTATGCTGGCTATGTCCCTGTCAATGAAACAAATGGCAGGGCTCTCTTTTACTGGTTTTATGAGGCTGCTACTAACCCAGATCTAAAACCTCTTGTTTTGTGGCTTAATGGAGGTCCTGGGTGCTCTTCTGTGGGATATGGAGCAACCCAAGAGATTGGTCCATTCATAGTGGAAACTGATGGCCATGGACTTAGATTTAATAACTACTCATGGAATAAAGAGGCCAACATGCTGTTCTTGGAATCTCCAGTTGGGGTTGGCTTTTCATACTCCAATACAACTACTGATTATGATAATCTCGGAGATGCCTTTACAGCAAATGATGCATATGCGTTTCTGCACAAGTGGTTTCTCAAGTTCCCATCATACAGAACAAGAACCTTCTACATTGCAGGGGAGAGTTATGCAGGAAAATATGTTCCGGAGCTGGCCGAGCTCATCAATGACAAGAATACAGATCCTTCCCTCCATATTGATCTCAAGGGAGTCCTGCTGGGTAATCCTGAAACATCAGATGCTGAGGATTGGAGAGGTATAGTGGATTATGCTTGGAGTCATGCTGTGATATCAGACGAAACTCACAAAGTTATAAGCGAAAACTGTGATTTTGACGGTGATACTTGGAGTAATAAGATCTGTGCTCAAGCTGTGGATGAAGTACTAAAACAATACAAGGAGATAGACATTTACAGCCTTTATACCTCAGTCTGCATCGGTGATACAGCAAGTTCAAATGATGGTTCGATGCAAATGCCAAGAATTATGGGTGGCTATGATCCGTGTCTGGATGATTATTCAAGAAAATTCTATAACAGAGCAGATGTGCAAAAGGCCCTCCATGTTAGTAATGGTCCCCAGCTCAAGAACTGGAGCATCTGCAGTACCAAAATATTTCATGAGTGGGTTGATTCAAAGCCATCTGTTCTTCCTATATACAAGAAACTCATTGCAGCAGGAATGAGAATATGGGTGTACAGTGGAGACACGGATGGAAGAGTTCCTGTGCTGTCCACGAGATATAGCATAAGTGCTCTGGGACTGCCTATTACAAAAGCATGGACACCGTGGTACCACCAAAAGCAGGTGAGTGGGTGGTTTCAAGAATATAAGGGGCTTACATTTGCAACATTCAGAGGAGCCGGGCATGCAGTTCCTTGCTTCAAACCAAGCAACTCACTGGCTTTATTCACCTCTTTTCTCCTTGGGGAATCTCTGCCTTCTGCTCGATGA

Protein Analysis

470

Amino Acids

52.53

Weight (kDa)

5.84

Isoelectric Point (pI)

29.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 52 - 462 1.2e-141 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 773
AarI CACCTGC 1 cut(s) 1255
Acc36I ACCTGC 2 cut(s) 159, 1255
Acc65I GGTACC 1 cut(s) 1251
AccB1I GGYRCC 1 cut(s) 1251
AccI GTMKAC 1 cut(s) 171
AccIII TCCGGA 2 cut(s) 76, 604
AciI CCGC 1 cut(s) 94
AclWI GGATC 3 cut(s) 144, 635, 953
AcoI YGGCCR 3 cut(s) 157, 364, 612
AcsI RAATTY 2 cut(s) 4, 986
AfaI GTAC 4 cut(s) 843, 1066, 1157, 1253
AfiI CCNNNNNNNGG 2 cut(s) 43, 1264
AgsI TTSAA 5 cut(s) 915, 981, 1095, 1283, 1345
AjnI CCWGG 2 cut(s) 154, 298
AjuI GAANNNNNNNTTGG 4 cut(s) 401, 417, 433, 449
AloI GAACNNNNNNTCC 2 cut(s) 585, 617
AluBI AGCT 4 cut(s) 610, 619, 830, 1042
AluI AGCT 4 cut(s) 610, 619, 830, 1042
Alw21I GWGCWC 4 cut(s) 308, 621, 826, 1216
Alw26I GTCTC 1 cut(s) 1158
AlwI GGATC 3 cut(s) 144, 635, 953
AlwNI CAGNNNCTG 1 cut(s) 701
Aor13HI TCCGGA 2 cut(s) 76, 604
AoxI GGCC 5 cut(s) 157, 364, 405, 612, 1014
ApeKI GCWGC 2 cut(s) 248, 1133
ApoI RAATTY 2 cut(s) 4, 986
Asp718I GGTACC 1 cut(s) 1251
AspS9I GGNCC 4 cut(s) 296, 341, 1015, 1034
AsuC2I CCSGG 1 cut(s) 1324
AsuHPI GGTGA 4 cut(s) 809, 911, 1279, 1363
AvaII GGWCC 3 cut(s) 296, 341, 1034
BaeI ACNNNNGTAYC 2 cut(s) 1235, 1268
BalI TGGCCA 2 cut(s) 159, 366
BanI GGYRCC 1 cut(s) 1251
BanII GRGCYC 2 cut(s) 226, 621
BauI CACGAG 1 cut(s) 1195
Bbv12I GWGCWC 4 cut(s) 308, 621, 826, 1216
BbvCI CCTCAGC 1 cut(s) 702
BbvI GCAGC 2 cut(s) 235, 1145
BccI CCATC 5 cut(s) 356, 553, 914, 1108, 1166
BcgI CGANNNNNNTGC 2 cut(s) 916, 950
BciT130I CCWGG 2 cut(s) 156, 300
BclI TGATCA 1 cut(s) 114
BcnI CCSGG 1 cut(s) 1324
BcoDI GTCTC 1 cut(s) 1158
BfaI CTAG 1 cut(s) 84
BfmI CTRYAG 2 cut(s) 168, 1060
BfuAI ACCTGC 2 cut(s) 159, 1255
BglII AGATCT 2 cut(s) 262, 816
BisI GCNGC 2 cut(s) 249, 1134
BlsI GCNGC 2 cut(s) 250, 1135
BmcAI AGTACT 1 cut(s) 843
Bme1390I CCNGG 3 cut(s) 156, 300, 1324
Bme18I GGWCC 3 cut(s) 296, 341, 1034
BmgT120I GGNCC 4 cut(s) 296, 341, 1015, 1034
BmiI GGNNCC 3 cut(s) 1036, 1253, 1321
BmrFI CCNGG 3 cut(s) 156, 300, 1324
BmsI GCATC 7 cut(s) 70, 478, 496, 688, 903, 918, 1065
BpmI CTGGAG 2 cut(s) 414, 1072
Bpu10I CCTNAGC 1 cut(s) 702
BpuEI CTTGAG 4 cut(s) 521, 647, 810, 1028
BpuMI CCSGG 1 cut(s) 1324
BsaJI CCNNGG 4 cut(s) 299, 367, 1247, 1384
BsaWI WCCGGW 2 cut(s) 76, 604
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 1264
Bse1I ACTGG 4 cut(s) 239, 431, 1055, 1365
Bse3DI GCAATG 2 cut(s) 570, 1128
BseAI TCCGGA 2 cut(s) 76, 604
BseBI CCWGG 2 cut(s) 156, 300
BseDI CCNNGG 4 cut(s) 299, 367, 1247, 1384
BseGI GGATG 4 cut(s) 85, 841, 976, 1177
BseLI CCNNNNNNNGG 2 cut(s) 43, 1264
BseMI GCAATG 2 cut(s) 570, 1128
BseMII CTCAG 3 cut(s) 96, 693, 900
BseNI ACTGG 4 cut(s) 239, 431, 1055, 1365
BseRI GAGGAG 1 cut(s) 1332
BseXI GCAGC 2 cut(s) 235, 1145
BseYI CCCAGC 2 cut(s) 676, 1038
BsgI GTGCAG 1 cut(s) 506
BshFI GGCC 5 cut(s) 159, 366, 407, 614, 1016
BshNI GGYRCC 1 cut(s) 1251
BsiHKAI GWGCWC 4 cut(s) 308, 621, 826, 1216
BsiSI CCGG 4 cut(s) 77, 178, 605, 1323
BslFI GGGAC 3 cut(s) 182, 1020, 1233
BslI CCNNNNNNNGG 2 cut(s) 43, 1264
BsmAI GTCTC 1 cut(s) 1158
BsmFI GGGAC 3 cut(s) 182, 1020, 1233
BsnI GGCC 5 cut(s) 159, 366, 407, 614, 1016
Bsp1286I GDGCHC 5 cut(s) 226, 308, 621, 826, 1216
Bsp13I TCCGGA 2 cut(s) 76, 604
Bsp1407I TGTACA 1 cut(s) 1155
Bsp143I GATC 7 cut(s) 114, 136, 262, 640, 658, 816, 958
Bsp19I CCATGG 1 cut(s) 367
BspACI CCGC 1 cut(s) 94
BspANI GGCC 5 cut(s) 159, 366, 407, 614, 1016
BspCNI CTCAG 3 cut(s) 97, 694, 899
BspEI TCCGGA 2 cut(s) 76, 604
BspHI TCATGA 1 cut(s) 1078
BspLI GGNNCC 3 cut(s) 1036, 1253, 1321
BspMAI CTGCAG 1 cut(s) 1064
BspMI ACCTGC 2 cut(s) 159, 1255
BspPI GGATC 3 cut(s) 144, 635, 953
BspQI GCTCTTC 1 cut(s) 313
BspT107I GGYRCC 1 cut(s) 1251
BsrDI GCAATG 2 cut(s) 570, 1128
BsrGI TGTACA 1 cut(s) 1155
BsrI ACTGG 4 cut(s) 239, 431, 1055, 1365
BssECI CCNNGG 4 cut(s) 299, 367, 1247, 1384
BssMI GATC 7 cut(s) 114, 136, 262, 640, 658, 816, 958
BssSI CACGAG 1 cut(s) 1195
BssT1I CCWWGG 2 cut(s) 367, 1384
Bst2BI CACGAG 1 cut(s) 1195
Bst2UI CCWGG 2 cut(s) 156, 300
Bst4CI ACNGT 6 cut(s) 28, 55, 785, 797, 1160, 1248
Bst6I CTCTTC 2 cut(s) 313, 1171
BstAPI GCANNNNNTGC 1 cut(s) 506
BstAUI TGTACA 1 cut(s) 1155
BstC8I GCNNGC 5 cut(s) 161, 165, 190, 612, 1329
BstDEI CTNAG 4 cut(s) 105, 374, 702, 886
BstDSI CCRYGG 2 cut(s) 367, 1247
BstF5I GGATG 4 cut(s) 85, 841, 976, 1177
BstKTI GATC 7 cut(s) 117, 139, 265, 643, 661, 819, 961
BstMAI GTCTC 1 cut(s) 1158
BstMBI GATC 7 cut(s) 114, 136, 262, 640, 658, 816, 958
BstMWI GCNNNNNNNGC 5 cut(s) 160, 497, 506, 616, 1328
BstNI CCWGG 2 cut(s) 156, 300
BstNSI RCATGY 2 cut(s) 415, 1331
BstSCI CCNGG 3 cut(s) 154, 298, 1322
BstSFI CTRYAG 2 cut(s) 168, 1060
BstV1I GCAGC 2 cut(s) 235, 1145
BstX2I RGATCY 4 cut(s) 136, 262, 640, 816
BstXI CCANNNNNNTGG 1 cut(s) 338
BstYI RGATCY 4 cut(s) 136, 262, 640, 816
BsuRI GGCC 5 cut(s) 159, 366, 407, 614, 1016
BtgI CCRYGG 2 cut(s) 367, 1247
BtsCI GGATG 4 cut(s) 85, 841, 976, 1177
BtsIMutI CAGTG 2 cut(s) 1165, 1358
BveI ACCTGC 2 cut(s) 159, 1255
Cac8I GCNNGC 5 cut(s) 161, 165, 190, 612, 1329
CaiI CAGNNNCTG 1 cut(s) 701
CciI TCATGA 1 cut(s) 1078
Cfr13I GGNCC 4 cut(s) 296, 341, 1015, 1034
Csp6I GTAC 4 cut(s) 842, 1065, 1156, 1252
CviAII CATG 8 cut(s) 368, 393, 412, 740, 1022, 1079, 1239, 1328
CviQI GTAC 4 cut(s) 842, 1065, 1156, 1252
DdeI CTNAG 4 cut(s) 105, 374, 702, 886
DpnI GATC 7 cut(s) 116, 138, 264, 642, 660, 818, 960
DpnII GATC 7 cut(s) 114, 136, 262, 640, 658, 816, 958
EaeI YGGCCR 3 cut(s) 157, 364, 612
Eam1104I CTCTTC 2 cut(s) 313, 1171
EarI CTCTTC 2 cut(s) 313, 1171
Ecl136II GAGCTC 1 cut(s) 619
Eco130I CCWWGG 2 cut(s) 367, 1384
Eco24I GRGCYC 2 cut(s) 226, 621
Eco32I GATATC 1 cut(s) 750
Eco47I GGWCC 3 cut(s) 296, 341, 1034
Eco53kI GAGCTC 1 cut(s) 619
EcoICRI GAGCTC 1 cut(s) 619
EcoO109I RGGNCCY 2 cut(s) 296, 1015
EcoRII CCWGG 2 cut(s) 154, 298
EcoRV GATATC 1 cut(s) 750
EcoT14I CCWWGG 2 cut(s) 367, 1384
EcoT22I ATGCAT 1 cut(s) 511
EcoT38I GRGCYC 2 cut(s) 226, 621
ErhI CCWWGG 2 cut(s) 367, 1384
FaeI CATG 8 cut(s) 371, 396, 415, 743, 1025, 1082, 1242, 1331
FaqI GGGAC 3 cut(s) 182, 1020, 1233
FatI CATG 8 cut(s) 367, 392, 411, 739, 1021, 1078, 1238, 1327
FauNDI CATATG 1 cut(s) 511
FbaI TGATCA 1 cut(s) 114
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 2 cut(s) 249, 1134
FokI GGATG 4 cut(s) 92, 848, 983, 1184
FriOI GRGCYC 2 cut(s) 226, 621
Fsp4HI GCNGC 2 cut(s) 249, 1134
FspBI CTAG 1 cut(s) 84
GluI GCNGC 2 cut(s) 249, 1134
GsaI CCCAGC 2 cut(s) 680, 1042
GsuI CTGGAG 2 cut(s) 414, 1072
HaeIII GGCC 5 cut(s) 159, 366, 407, 614, 1016
HapII CCGG 4 cut(s) 77, 178, 605, 1323
Hin1II CATG 8 cut(s) 371, 396, 415, 743, 1025, 1082, 1242, 1331
HinfI GANTC 6 cut(s) 130, 425, 669, 736, 1091, 1391
HpaII CCGG 4 cut(s) 77, 178, 605, 1323
HphI GGTGA 4 cut(s) 809, 911, 1279, 1363
Hpy166II GTNNAC 3 cut(s) 172, 1156, 1194
Hpy188I TCNGA 4 cut(s) 485, 697, 754, 1316
Hpy188III TCNNGA 9 cut(s) 77, 134, 605, 686, 968, 981, 1045, 1079, 1283
Hpy8I GTNNAC 3 cut(s) 172, 1156, 1194
HpyAV CCTTC 3 cut(s) 574, 654, 1410
HpyCH4III ACNGT 6 cut(s) 28, 55, 785, 797, 1160, 1248
HpyF10VI GCNNNNNNNGC 5 cut(s) 160, 497, 506, 616, 1328
HpyF3I CTNAG 4 cut(s) 105, 374, 702, 886
Hsp92II CATG 8 cut(s) 371, 396, 415, 743, 1025, 1082, 1242, 1331
Kpn2I TCCGGA 2 cut(s) 76, 604
KpnI GGTACC 1 cut(s) 1255
Ksp22I TGATCA 1 cut(s) 114
Kzo9I GATC 7 cut(s) 114, 136, 262, 640, 658, 816, 958
LguI GCTCTTC 1 cut(s) 313
LmnI GCTCC 4 cut(s) 323, 607, 1053, 1319
Lsp1109I GCAGC 2 cut(s) 235, 1145
LweI GCATC 7 cut(s) 70, 478, 496, 688, 903, 918, 1065
MaeI CTAG 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 142
MalI GATC 7 cut(s) 116, 138, 264, 642, 660, 818, 960
MboI GATC 7 cut(s) 114, 136, 262, 640, 658, 816, 958
MboII GAAGA 4 cut(s) 25, 300, 1100, 1188
MflI RGATCY 4 cut(s) 136, 262, 640, 816
MhlI GDGCHC 5 cut(s) 226, 308, 621, 826, 1216
MlsI TGGCCA 2 cut(s) 159, 366
MluCI AATT 5 cut(s) 4, 39, 110, 942, 986
MluNI TGGCCA 2 cut(s) 159, 366
MlyI GAGTC 3 cut(s) 139, 678, 745
Mox20I TGGCCA 2 cut(s) 159, 366
Mph1103I ATGCAT 1 cut(s) 511
MroI TCCGGA 2 cut(s) 76, 604
MscI TGGCCA 2 cut(s) 159, 366
MseI TTAA 2 cut(s) 288, 381
Msp20I TGGCCA 2 cut(s) 159, 366
MspI CCGG 4 cut(s) 77, 178, 605, 1323
MspR9I CCNGG 3 cut(s) 156, 300, 1324
MvaI CCWGG 2 cut(s) 156, 300
MwoI GCNNNNNNNGC 5 cut(s) 160, 497, 506, 616, 1328
NciI CCSGG 1 cut(s) 1324
NcoI CCATGG 1 cut(s) 367
NdeI CATATG 1 cut(s) 511
NdeII GATC 7 cut(s) 114, 136, 262, 640, 658, 816, 958
NlaIII CATG 8 cut(s) 371, 396, 415, 743, 1025, 1082, 1242, 1331
NlaIV GGNNCC 3 cut(s) 1036, 1253, 1321
NmeAIII GCCGAG 1 cut(s) 640
NmuCI GTSAC 1 cut(s) 142
NsiI ATGCAT 1 cut(s) 511
NspI RCATGY 2 cut(s) 415, 1331
PaeI GCATGC 1 cut(s) 1331
PagI TCATGA 1 cut(s) 1078
PaqCI CACCTGC 1 cut(s) 1255
PciSI GCTCTTC 1 cut(s) 313
PfeI GAWTC 3 cut(s) 425, 1091, 1391
PkrI GCNGC 2 cut(s) 250, 1135
PleI GAGTC 3 cut(s) 138, 677, 744
PpsI GAGTC 3 cut(s) 138, 677, 744
PpuMI RGGWCCY 1 cut(s) 296
PsiI TTATAA 1 cut(s) 773
Psp124BI GAGCTC 1 cut(s) 621
Psp5II RGGWCCY 1 cut(s) 296
Psp6I CCWGG 2 cut(s) 154, 298
PspFI CCCAGC 2 cut(s) 676, 1038
PspGI CCWGG 2 cut(s) 154, 298
PspN4I GGNNCC 3 cut(s) 1036, 1253, 1321
PspPI GGNCC 4 cut(s) 296, 341, 1015, 1034
PspPPI RGGWCCY 1 cut(s) 296
PstI CTGCAG 1 cut(s) 1064
PstNI CAGNNNCTG 1 cut(s) 701
PsuI RGATCY 4 cut(s) 136, 262, 640, 816
RsaI GTAC 4 cut(s) 843, 1066, 1157, 1253
RsaNI GTAC 4 cut(s) 842, 1065, 1156, 1252
SacI GAGCTC 1 cut(s) 621
SapI GCTCTTC 1 cut(s) 313
SaqAI TTAA 2 cut(s) 288, 381
SatI GCNGC 2 cut(s) 249, 1134
Sau3AI GATC 7 cut(s) 114, 136, 262, 640, 658, 816, 958
Sau96I GGNCC 4 cut(s) 296, 341, 1015, 1034
ScaI AGTACT 1 cut(s) 843
SchI GAGTC 3 cut(s) 139, 678, 745
ScrFI CCNGG 3 cut(s) 156, 300, 1324
SduI GDGCHC 5 cut(s) 226, 308, 621, 826, 1216
SfaNI GCATC 7 cut(s) 70, 478, 496, 688, 903, 918, 1065
SfcI CTRYAG 2 cut(s) 168, 1060
SinI GGWCC 3 cut(s) 296, 341, 1034
SmlI CTYRAG 4 cut(s) 536, 662, 825, 1043
SmoI CTYRAG 4 cut(s) 536, 662, 825, 1043
SphI GCATGC 1 cut(s) 1331
Sse9I AATT 5 cut(s) 4, 39, 110, 942, 986
SsiI CCGC 1 cut(s) 94
SspI AATATT 1 cut(s) 1074
SspMI CTAG 1 cut(s) 84
SstI GAGCTC 1 cut(s) 621
StyD4I CCNGG 3 cut(s) 154, 298, 1322
StyI CCWWGG 2 cut(s) 367, 1384
TaaI ACNGT 6 cut(s) 28, 55, 785, 797, 1160, 1248
TaqI TCGA 3 cut(s) 60, 926, 1408
TasI AATT 5 cut(s) 4, 39, 110, 942, 986
TatI WGTACW 2 cut(s) 841, 1155
TfiI GAWTC 3 cut(s) 425, 1091, 1391
Tru1I TTAA 2 cut(s) 288, 381
Tru9I TTAA 2 cut(s) 288, 381
TscAI CASTG 2 cut(s) 1165, 1365
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 2 cut(s) 248, 1133
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 6 cut(s) 17, 222, 337, 438, 852, 1067
TspGWI ACGGA 2 cut(s) 951, 1184
TspRI CASTG 2 cut(s) 1165, 1365
VpaK11BI GGWCC 3 cut(s) 296, 341, 1034
XapI RAATTY 2 cut(s) 4, 986
XceI RCATGY 2 cut(s) 415, 1331
XcmI CCANNNNNNNNNTGG 1 cut(s) 1028
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 1 cut(s) 84
ZrmI AGTACT 1 cut(s) 843
Zsp2I ATGCAT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.