Rw5G005400

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003577
Physical Location & Seq
Reverse (-)
5663257 .. 5665981
2725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G005400.1

Sequence Viewer

Length: 1446 bp
ATGGAGTTCGTAGTAAAAGCGACAGCCTTCTTCTTCATTCTGGCAACTATTGTGGAGCCTCAGCCTGTTTTGGGATCTAGAAGAATATTGCAACAACAGACTGGGATTAGTACTAGTAGTGATGAGGATCTTGTGACCAACTTGCCTCGCCAGCCTGCCGTAGACTTCAAGCACTATGCCGGCTATGTCACTGTGAACGAAACAAAAGGACGGGCACTCTTTTACTGGTTCTTTGAGGCTGCTACCACTCCAGATCAAAAACCTCTTGTTTTGTGGCTTAATGGAGGTCCTGGGTGCTCTTCTGTGGGATATGGAGCCAGCCAAGAGATTGGTCCATTCCTATTGGACATTAAGAGCCAGGGACTCATATTTAATAACTACTCATGGAATAAAGAGGCCAACGTGCTATTCGTGGAATCTCCGATTGGGGTTGGCTTTTCATACACCAACACAACTTCTGATTTTGATAAGCTAGGAGATAACTTCACAGCGCAAGATTCATACACATTCCTGCATAAGTGGTTTCTCAAATTCCCATCATACAGAAACCGGACCTTGTATATTGCAGGCGAGAGTTATGCAGGAAAATATATTCCGGAGCTAGCTGATTTCATCTTTGATAGGAACTCAGATCCTTCCCTCCATATTGATCTCAAGGGTATCCTGATGGGTAATCCTGAAACAGATGATGCTGAGGATTGGAGAGGTCTGGTGGATTATGCTTGGAGTCATGCTGTGGTTTCGGACGAAACTCACAAATTAATAAGCGAGTACTGTGATTTCCACAGTAATGATACTTGGGAAAATAAAAATTGTACACAAGGTGTGGATGAAGTATTTAAACAGTACAAGGAGATAGATATATACAGCCTTTACACCCCAGCCTGCACTACAAATGATATAGCAACTTCTAATACTGCTGATTCTAAAGTTCAAGTCATGACGATGAAGCACACATCTACAATGATACCAAGGATTATGGGAGGCTATGATGTATGTCTGGATGGTTATTCGAAAAAATTCTTCAACAGAGCAGATGTGCAAAAGGCCCTCCATGCCAGTGATGGTCTCCAGCTCAAGAACTGGAGCATCTGCAATAATCAAATATTTACCAATTGGTCAGATTCAAAGCCATCTCTTCTTCCCATATACAAGAAACTCATTGCAGCAGGACTAAGAATATGGTTGTACAGTGGAGACACGGATGGAAGAGTACCTGTGCTGTCCACAAGATATAGCTTAGGTGCTCTAGGGCTGCCTATTACAAAGACATGGAGACCTTGGTACCATCAGAAGCAGGTGAGTGGTTGGTATCAAGAATATGAGGGGCTTACATATGCAACAGTTAGAGGAGCTGGGCATGATGTTCCCTCCTTCAAACCAGCAGAGTCATTGGTTCTATTCACATCTTTTCTCCTTGGGGAATCTCCATCTTCGACTCGATGA

Protein Analysis

481

Amino Acids

54.24

Weight (kDa)

5.53

Isoelectric Point (pI)

33.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 52 - 473 2.2e-140 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1288
Acc36I ACCTGC 1 cut(s) 1288
Acc65I GGTACC 1 cut(s) 1284
AccB1I GGYRCC 1 cut(s) 1284
AccI GTMKAC 1 cut(s) 162
AccIII TCCGGA 1 cut(s) 595
AclWI GGATC 3 cut(s) 82, 135, 626
AcsI RAATTY 2 cut(s) 530, 1019
AdeI CACNNNGTG 1 cut(s) 824
AfaI GTAC 7 cut(s) 112, 773, 817, 848, 1190, 1215, 1286
AfiI CCNNNNNNNGG 1 cut(s) 71
AgsI TTSAA 5 cut(s) 169, 935, 1027, 1128, 1378
AhlI ACTAGT 1 cut(s) 113
AjnI CCWGG 2 cut(s) 289, 357
AjuI GAANNNNNNNTTGG 4 cut(s) 392, 408, 424, 440
AluBI AGCT 6 cut(s) 472, 601, 605, 1075, 1239, 1355
AluI AGCT 6 cut(s) 472, 601, 605, 1075, 1239, 1355
Alw21I GWGCWC 2 cut(s) 299, 1249
Alw26I GTCTC 3 cut(s) 1073, 1191, 1270
AlwI GGATC 3 cut(s) 82, 135, 626
Aor13HI TCCGGA 1 cut(s) 595
AoxI GGCC 2 cut(s) 396, 1047
ApeKI GCWGC 3 cut(s) 239, 1166, 1255
ApoI RAATTY 2 cut(s) 530, 1019
AseI ATTAAT 1 cut(s) 761
Asp700I GAANNNNTTC 1 cut(s) 1019
Asp718I GGTACC 1 cut(s) 1284
AspLEI GCGC 1 cut(s) 493
AspS9I GGNCC 4 cut(s) 287, 332, 552, 1048
AsuHPI GGTGA 1 cut(s) 1312
AsuII TTCGAA 1 cut(s) 1013
AsuNHI GCTAGC 1 cut(s) 601
AvaII GGWCC 3 cut(s) 287, 332, 552
BaeGI GKGCMC 1 cut(s) 217
BaeI ACNNNNGTAYC 2 cut(s) 1268, 1301
BanI GGYRCC 1 cut(s) 1284
Bbv12I GWGCWC 2 cut(s) 299, 1249
BbvCI CCTCAGC 2 cut(s) 60, 693
BbvI GCAGC 3 cut(s) 226, 1178, 1242
BccI CCATC 8 cut(s) 544, 661, 998, 1058, 1141, 1199, 1296, 1438
BceAI ACGGC 1 cut(s) 143
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BciT130I CCWGG 2 cut(s) 291, 359
BciVI GTATCC 1 cut(s) 671
BcoDI GTCTC 3 cut(s) 1073, 1191, 1270
BcuI ACTAGT 1 cut(s) 113
BfaI CTAG 5 cut(s) 78, 114, 473, 602, 1250
BfuAI ACCTGC 1 cut(s) 1288
BfuI GTATCC 1 cut(s) 671
BisI GCNGC 3 cut(s) 240, 1167, 1256
BlsI GCNGC 3 cut(s) 241, 1168, 1257
BmcAI AGTACT 2 cut(s) 112, 773
Bme1390I CCNGG 2 cut(s) 291, 359
Bme18I GGWCC 3 cut(s) 287, 332, 552
BmgT120I GGNCC 4 cut(s) 287, 332, 552, 1048
BmiI GGNNCC 3 cut(s) 57, 316, 1286
BmrFI CCNGG 2 cut(s) 291, 359
BmrI ACTGGG 1 cut(s) 111
BmsI GCATC 2 cut(s) 679, 1098
BmtI GCTAGC 1 cut(s) 605
BmuI ACTGGG 1 cut(s) 111
BpmI CTGGAG 3 cut(s) 234, 1055, 1105
Bpu10I CCTNAGC 3 cut(s) 60, 693, 1240
Bpu14I TTCGAA 1 cut(s) 1013
BpuEI CTTGAG 2 cut(s) 638, 1061
BsaBI GATNNNNATC 1 cut(s) 126
BsaI GGTCTC 2 cut(s) 1073, 1270
BsaJI CCNNGG 5 cut(s) 290, 358, 971, 1280, 1417
BsaWI WCCGGW 2 cut(s) 549, 595
Bsc4I CCNNNNNNNGG 1 cut(s) 71
Bse118I RCCGGY 1 cut(s) 179
Bse1I ACTGG 4 cut(s) 106, 230, 1059, 1088
Bse3DI GCAATG 1 cut(s) 1161
Bse8I GATNNNNATC 1 cut(s) 126
BseAI TCCGGA 1 cut(s) 595
BseBI CCWGG 2 cut(s) 291, 359
BseDI CCNNGG 5 cut(s) 290, 358, 971, 1280, 1417
BseGI GGATG 3 cut(s) 835, 1009, 1210
BseJI GATNNNNATC 1 cut(s) 126
BseLI CCNNNNNNNGG 1 cut(s) 71
BseMI GCAATG 1 cut(s) 1161
BseMII CTCAG 3 cut(s) 74, 642, 684
BseNI ACTGG 4 cut(s) 106, 230, 1059, 1088
BseRI GAGGAG 1 cut(s) 1365
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 3 cut(s) 226, 1178, 1242
BseYI CCCAGC 2 cut(s) 880, 1355
BsgI GTGCAG 1 cut(s) 871
BshFI GGCC 2 cut(s) 398, 1049
BshNI GGYRCC 1 cut(s) 1284
BsiHKAI GWGCWC 2 cut(s) 299, 1249
BsiSI CCGG 3 cut(s) 180, 550, 596
BslFI GGGAC 1 cut(s) 375
BslI CCNNNNNNNGG 1 cut(s) 71
BsmAI GTCTC 3 cut(s) 1073, 1191, 1270
BsmFI GGGAC 1 cut(s) 375
BsnI GGCC 2 cut(s) 398, 1049
Bso31I GGTCTC 2 cut(s) 1073, 1270
Bsp119I TTCGAA 1 cut(s) 1013
Bsp1286I GDGCHC 3 cut(s) 217, 299, 1249
Bsp13I TCCGGA 1 cut(s) 595
Bsp1407I TGTACA 2 cut(s) 815, 1188
Bsp143I GATC 5 cut(s) 74, 127, 253, 631, 649
BspANI GGCC 2 cut(s) 398, 1049
BspCNI CTCAG 3 cut(s) 73, 641, 685
BspEI TCCGGA 1 cut(s) 595
BspHI TCATGA 1 cut(s) 939
BspLI GGNNCC 3 cut(s) 57, 316, 1286
BspMI ACCTGC 1 cut(s) 1288
BspOI GCTAGC 1 cut(s) 605
BspPI GGATC 3 cut(s) 82, 135, 626
BspQI GCTCTTC 1 cut(s) 304
BspT104I TTCGAA 1 cut(s) 1013
BspT107I GGYRCC 1 cut(s) 1284
BspTNI GGTCTC 2 cut(s) 1073, 1270
BsrDI GCAATG 1 cut(s) 1161
BsrFI RCCGGY 1 cut(s) 179
BsrGI TGTACA 2 cut(s) 815, 1188
BsrI ACTGG 4 cut(s) 106, 230, 1059, 1088
BssAI RCCGGY 1 cut(s) 179
BssECI CCNNGG 5 cut(s) 290, 358, 971, 1280, 1417
BssMI GATC 5 cut(s) 74, 127, 253, 631, 649
BssT1I CCWWGG 3 cut(s) 971, 1280, 1417
Bst2UI CCWGG 2 cut(s) 291, 359
Bst4CI ACNGT 6 cut(s) 193, 776, 788, 846, 1193, 1345
Bst6I CTCTTC 3 cut(s) 304, 1143, 1204
BstAUI TGTACA 2 cut(s) 815, 1188
BstBI TTCGAA 1 cut(s) 1013
BstC8I GCNNGC 7 cut(s) 152, 156, 181, 319, 568, 603, 886
BstDEI CTNAG 5 cut(s) 60, 628, 693, 1175, 1240
BstF5I GGATG 3 cut(s) 835, 1009, 1210
BstHHI GCGC 1 cut(s) 493
BstKTI GATC 5 cut(s) 77, 130, 256, 634, 652
BstMAI GTCTC 3 cut(s) 1073, 1191, 1270
BstMBI GATC 5 cut(s) 74, 127, 253, 631, 649
BstMWI GCNNNNNNNGC 2 cut(s) 151, 1055
BstNI CCWGG 2 cut(s) 291, 359
BstSCI CCNGG 2 cut(s) 289, 357
BstSLI GKGCMC 1 cut(s) 217
BstV1I GCAGC 3 cut(s) 226, 1178, 1242
BstX2I RGATCY 3 cut(s) 74, 127, 631
BstXI CCANNNNNNTGG 1 cut(s) 329
BstYI RGATCY 3 cut(s) 74, 127, 631
BsuI GTATCC 1 cut(s) 671
BsuRI GGCC 2 cut(s) 398, 1049
BtsCI GGATG 3 cut(s) 835, 1009, 1210
BtsIMutI CAGTG 3 cut(s) 189, 1066, 1198
BveI ACCTGC 1 cut(s) 1288
Cac8I GCNNGC 7 cut(s) 152, 156, 181, 319, 568, 603, 886
CciI TCATGA 1 cut(s) 939
CfoI GCGC 1 cut(s) 493
Cfr10I RCCGGY 1 cut(s) 179
Cfr13I GGNCC 4 cut(s) 287, 332, 552, 1048
Csp6I GTAC 7 cut(s) 111, 772, 816, 847, 1189, 1214, 1285
CspCI CAANNNNNGTGG 2 cut(s) 33, 68
CviAII CATG 6 cut(s) 384, 731, 940, 1055, 1272, 1361
CviQI GTAC 7 cut(s) 111, 772, 816, 847, 1189, 1214, 1285
DdeI CTNAG 5 cut(s) 60, 628, 693, 1175, 1240
DpnI GATC 5 cut(s) 76, 129, 255, 633, 651
DpnII GATC 5 cut(s) 74, 127, 253, 631, 649
DraI TTTAAA 1 cut(s) 841
DraIII CACNNNGTG 1 cut(s) 824
Eam1104I CTCTTC 3 cut(s) 304, 1143, 1204
EarI CTCTTC 3 cut(s) 304, 1143, 1204
Eco130I CCWWGG 3 cut(s) 971, 1280, 1417
Eco31I GGTCTC 2 cut(s) 1073, 1270
Eco47I GGWCC 3 cut(s) 287, 332, 552
EcoO109I RGGNCCY 2 cut(s) 287, 1048
EcoRII CCWGG 2 cut(s) 289, 357
EcoT14I CCWWGG 3 cut(s) 971, 1280, 1417
ErhI CCWWGG 3 cut(s) 971, 1280, 1417
FaeI CATG 6 cut(s) 387, 734, 943, 1058, 1275, 1364
FaqI GGGAC 1 cut(s) 375
FatI CATG 6 cut(s) 383, 730, 939, 1054, 1271, 1360
FauNDI CATATG 1 cut(s) 1336
FblI GTMKAC 1 cut(s) 162
Fnu4HI GCNGC 3 cut(s) 240, 1167, 1256
FokI GGATG 3 cut(s) 842, 1016, 1217
Fsp4HI GCNGC 3 cut(s) 240, 1167, 1256
FspBI CTAG 5 cut(s) 78, 114, 473, 602, 1250
GlaI GCGC 1 cut(s) 492
GluI GCNGC 3 cut(s) 240, 1167, 1256
GsaI CCCAGC 2 cut(s) 884, 1359
GsuI CTGGAG 3 cut(s) 234, 1055, 1105
HaeIII GGCC 2 cut(s) 398, 1049
HapII CCGG 3 cut(s) 180, 550, 596
HhaI GCGC 1 cut(s) 493
Hin1II CATG 6 cut(s) 387, 734, 943, 1058, 1275, 1364
Hin6I GCGC 1 cut(s) 491
HinP1I GCGC 1 cut(s) 491
HinfI GANTC 9 cut(s) 363, 416, 497, 727, 923, 1124, 1388, 1424, 1438
HpaII CCGG 3 cut(s) 180, 550, 596
HphI GGTGA 1 cut(s) 1312
Hpy166II GTNNAC 4 cut(s) 163, 196, 818, 1227
Hpy188I TCNGA 6 cut(s) 423, 460, 631, 745, 1123, 1293
Hpy188III TCNNGA 9 cut(s) 78, 251, 596, 664, 677, 940, 1001, 1078, 1316
Hpy8I GTNNAC 4 cut(s) 163, 196, 818, 1227
HpyAV CCTTC 3 cut(s) 37, 645, 1384
HpyCH4III ACNGT 6 cut(s) 193, 776, 788, 846, 1193, 1345
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 9 cut(s) 91, 514, 566, 581, 888, 1042, 1095, 1166, 1340
HpyF10VI GCNNNNNNNGC 2 cut(s) 151, 1055
HpyF3I CTNAG 5 cut(s) 60, 628, 693, 1175, 1240
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 6 cut(s) 387, 734, 943, 1058, 1275, 1364
HspAI GCGC 1 cut(s) 491
Kpn2I TCCGGA 1 cut(s) 595
KpnI GGTACC 1 cut(s) 1288
KroI GCCGGC 1 cut(s) 179
KroNI GCCGGC 1 cut(s) 181
Kzo9I GATC 5 cut(s) 74, 127, 253, 631, 649
LguI GCTCTTC 1 cut(s) 304
LmnI GCTCC 5 cut(s) 55, 314, 598, 1086, 1352
Lsp1109I GCAGC 3 cut(s) 226, 1178, 1242
LweI GCATC 2 cut(s) 679, 1098
MaeI CTAG 5 cut(s) 78, 114, 473, 602, 1250
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 2 cut(s) 133, 187
MalI GATC 5 cut(s) 76, 129, 255, 633, 651
MboI GATC 5 cut(s) 74, 127, 253, 631, 649
MboII GAAGA 9 cut(s) 22, 25, 93, 291, 1015, 1130, 1133, 1221, 1425
MfeI CAATTG 1 cut(s) 1114
MflI RGATCY 3 cut(s) 74, 127, 631
MhlI GDGCHC 3 cut(s) 217, 299, 1249
MluCI AATT 5 cut(s) 530, 758, 811, 1019, 1114
MlyI GAGTC 4 cut(s) 357, 736, 1397, 1432
MroI TCCGGA 1 cut(s) 595
MroNI GCCGGC 1 cut(s) 179
MroXI GAANNNNTTC 1 cut(s) 1019
MseI TTAA 5 cut(s) 279, 351, 372, 761, 840
MslI CAYNNNNRTG 3 cut(s) 789, 944, 1059
MspI CCGG 3 cut(s) 180, 550, 596
MspR9I CCNGG 2 cut(s) 291, 359
MunI CAATTG 1 cut(s) 1114
MvaI CCWGG 2 cut(s) 291, 359
MwoI GCNNNNNNNGC 2 cut(s) 151, 1055
NaeI GCCGGC 1 cut(s) 181
NdeI CATATG 1 cut(s) 1336
NdeII GATC 5 cut(s) 74, 127, 253, 631, 649
NgoMIV GCCGGC 1 cut(s) 179
NheI GCTAGC 1 cut(s) 601
NlaIII CATG 6 cut(s) 387, 734, 943, 1058, 1275, 1364
NlaIV GGNNCC 3 cut(s) 57, 316, 1286
NmuCI GTSAC 2 cut(s) 133, 187
NspV TTCGAA 1 cut(s) 1013
PagI TCATGA 1 cut(s) 939
PaqCI CACCTGC 1 cut(s) 1288
PciSI GCTCTTC 1 cut(s) 304
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PdiI GCCGGC 1 cut(s) 181
PdmI GAANNNNTTC 1 cut(s) 1019
PfeI GAWTC 5 cut(s) 416, 497, 923, 1124, 1424
PkrI GCNGC 3 cut(s) 241, 1168, 1257
PleI GAGTC 4 cut(s) 357, 735, 1396, 1432
PpsI GAGTC 4 cut(s) 357, 735, 1396, 1432
PpuMI RGGWCCY 1 cut(s) 287
PshBI ATTAAT 1 cut(s) 761
Psp5II RGGWCCY 1 cut(s) 287
Psp6I CCWGG 2 cut(s) 289, 357
PspFI CCCAGC 2 cut(s) 880, 1355
PspGI CCWGG 2 cut(s) 289, 357
PspN4I GGNNCC 3 cut(s) 57, 316, 1286
PspPI GGNCC 4 cut(s) 287, 332, 552, 1048
PspPPI RGGWCCY 1 cut(s) 287
PsuI RGATCY 3 cut(s) 74, 127, 631
RsaI GTAC 7 cut(s) 112, 773, 817, 848, 1190, 1215, 1286
RsaNI GTAC 7 cut(s) 111, 772, 816, 847, 1189, 1214, 1285
RseI CAYNNNNRTG 3 cut(s) 789, 944, 1059
SapI GCTCTTC 1 cut(s) 304
SaqAI TTAA 5 cut(s) 279, 351, 372, 761, 840
SatI GCNGC 3 cut(s) 240, 1167, 1256
Sau3AI GATC 5 cut(s) 74, 127, 253, 631, 649
Sau96I GGNCC 4 cut(s) 287, 332, 552, 1048
ScaI AGTACT 2 cut(s) 112, 773
SchI GAGTC 4 cut(s) 357, 736, 1397, 1432
ScrFI CCNGG 2 cut(s) 291, 359
SduI GDGCHC 3 cut(s) 217, 299, 1249
SfaNI GCATC 2 cut(s) 679, 1098
SfuI TTCGAA 1 cut(s) 1013
SinI GGWCC 3 cut(s) 287, 332, 552
SmiMI CAYNNNNRTG 3 cut(s) 789, 944, 1059
SmlI CTYRAG 2 cut(s) 653, 1076
SmoI CTYRAG 2 cut(s) 653, 1076
SpeI ACTAGT 1 cut(s) 113
Sse9I AATT 5 cut(s) 530, 758, 811, 1019, 1114
SspI AATATT 2 cut(s) 87, 1107
SspMI CTAG 5 cut(s) 78, 114, 473, 602, 1250
StyD4I CCNGG 2 cut(s) 289, 357
StyI CCWWGG 3 cut(s) 971, 1280, 1417
TaaI ACNGT 6 cut(s) 193, 776, 788, 846, 1193, 1345
TaiI ACGT 1 cut(s) 405
TaqI TCGA 3 cut(s) 1013, 1436, 1441
TasI AATT 5 cut(s) 530, 758, 811, 1019, 1114
TatI WGTACW 5 cut(s) 110, 771, 815, 846, 1188
TfiI GAWTC 5 cut(s) 416, 497, 923, 1124, 1424
Tru1I TTAA 5 cut(s) 279, 351, 372, 761, 840
Tru9I TTAA 5 cut(s) 279, 351, 372, 761, 840
TscAI CASTG 3 cut(s) 196, 1066, 1198
TseFI GTSAC 2 cut(s) 133, 187
TseI GCWGC 3 cut(s) 239, 1166, 1255
Tsp45I GTSAC 2 cut(s) 133, 187
TspDTI ATGAA 6 cut(s) 25, 429, 489, 601, 846, 962
TspGWI ACGGA 1 cut(s) 1217
TspRI CASTG 3 cut(s) 196, 1066, 1198
VpaK11BI GGWCC 3 cut(s) 287, 332, 552
VspI ATTAAT 1 cut(s) 761
XapI RAATTY 2 cut(s) 530, 1019
XbaI TCTAGA 1 cut(s) 77
XcmI CCANNNNNNNNNTGG 1 cut(s) 1061
XmiI GTMKAC 1 cut(s) 162
XmnI GAANNNNTTC 1 cut(s) 1019
XspI CTAG 5 cut(s) 78, 114, 473, 602, 1250
ZrmI AGTACT 2 cut(s) 112, 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.