Rh5CG063400

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0003577
Physical Location & Seq
Forward (+)
4776008 .. 4778702
2695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG063400.1

Sequence Viewer

Length: 1164 bp
ATGCTATATGTTTCAGGTCCTGGGTGCTCTTCTGTGGGATATGGAGCAACCCAAGAGATTGGTCCATTCATAGTGGAAACTGATGGCCATGGACTTAGATTTAATAACTACTCATGGAATAAAGAGGCCAACATGCTGTTCTTGGAATCTCCAGTTGGGGTTGGCTTTTCATACTCCAATACAACTACTGATTATGATAATCTCGGAGATGCCTTTACAGCAAATGATGCATATGCGTTTCTGCACAAGTGGTTTCTCAAGTTCCCATCTTACAGAACAAGAACCTTCTACATTGCAGGGGAGAGTTATGCAGGAAAATATGTTCCGGAGCTGGCCGAGCTCATCAATGACAAGAATACAGATCCTTCCCTCCAAATTGATCTCAAGGGAGTCCTGCTGGGTAATCCTGAAACATCAGATGCTGAGGATTGGAGAGGTATAGTGGATTATGCTTGGAGTCATGCTGTGATATCAGACGAAACTCACAAAGTTATAAGCGAAAACTGTGATTTTGACGGTGATACTTGGAGTAATAAGATCTGTGCTCAAGCTGTGGATGAAGTACTAAAACAATACAAGGAGATAGACATTTACAGCCTTTATACCTCGGTCTGCATCGGTGATACAGCAAGTTCAAATGATGGTTCGATGCAAGTAATGATGATGAAGAACACATCTACAATGATGCCAAGAATTATGGGTGGCTATGATCCGTGTCTGGATGATTATTCAAGAAAATTCTATAACAGAGCAGATGTGCAAAAGGCCCTCCATGTTAGTAATGGTCCCCAGCTCAAGAACTGGAGCATCTGCAGTACCAAAATATTTCATGAGTGGGTTGATTCAAAGCCATCTGTTCTTCCTATATACAAGAAACTCATTGCAGCAGGAATGAGAATATGGGTGTACAGTGGAGACACGGATGGAAGAGTTCCTGTGCTGTCCACGAGATATAGCTTAAGTGCTCTGGGACTGCCTATTACAAAAGCATGGACACCGTGGTACCACCAAAAGCAGGTGAGTGGGTGGTTTCAAGAATATAAGGGCCTTACATTTGCAACATTCAGAGGAGCCGGCCATGCAGTTCCTTGCTTCAAACCAAGCAACTCACTGGCTTTATTCACCTCTTTTCTCCTTGGGGAATCTCTGCCTTCTGCTCGATGA

Protein Analysis

387

Amino Acids

43.3

Weight (kDa)

5.52

Isoelectric Point (pI)

24.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 6 - 379 7e-118 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 494
AarI CACCTGC 1 cut(s) 1006
Acc36I ACCTGC 1 cut(s) 1006
Acc65I GGTACC 1 cut(s) 1002
AccB1I GGYRCC 1 cut(s) 1002
AccIII TCCGGA 1 cut(s) 325
AclWI GGATC 2 cut(s) 356, 704
AcoI YGGCCR 3 cut(s) 85, 333, 1075
AcsI RAATTY 1 cut(s) 737
AfaI GTAC 4 cut(s) 564, 817, 908, 1004
AfiI CCNNNNNNNGG 1 cut(s) 1015
AflII CTTAAG 1 cut(s) 958
AgsI TTSAA 5 cut(s) 636, 732, 846, 1034, 1096
AjnI CCWGG 1 cut(s) 19
AjuI GAANNNNNNNTTGG 4 cut(s) 122, 138, 154, 170
AloI GAACNNNNNNTCC 2 cut(s) 306, 338
AluBI AGCT 5 cut(s) 331, 340, 551, 793, 957
AluI AGCT 5 cut(s) 331, 340, 551, 793, 957
Alw21I GWGCWC 4 cut(s) 29, 342, 547, 967
Alw26I GTCTC 1 cut(s) 909
AlwI GGATC 2 cut(s) 356, 704
AlwNI CAGNNNCTG 2 cut(s) 20, 422
Aor13HI TCCGGA 1 cut(s) 325
AoxI GGCC 6 cut(s) 85, 126, 333, 765, 1045, 1075
ApeKI GCWGC 1 cut(s) 884
ApoI RAATTY 1 cut(s) 737
Asp718I GGTACC 1 cut(s) 1002
AspS9I GGNCC 5 cut(s) 17, 62, 766, 785, 1045
AsuHPI GGTGA 4 cut(s) 530, 632, 1030, 1114
AvaII GGWCC 3 cut(s) 17, 62, 785
BaeI ACNNNNGTAYC 2 cut(s) 986, 1019
BalI TGGCCA 1 cut(s) 87
BanI GGYRCC 1 cut(s) 1002
BanII GRGCYC 1 cut(s) 342
BauI CACGAG 1 cut(s) 946
Bbv12I GWGCWC 4 cut(s) 29, 342, 547, 967
BbvCI CCTCAGC 1 cut(s) 423
BbvI GCAGC 1 cut(s) 896
BccI CCATC 5 cut(s) 77, 274, 635, 859, 917
BciT130I CCWGG 1 cut(s) 21
BcoDI GTCTC 1 cut(s) 909
BfmI CTRYAG 1 cut(s) 811
BfrI CTTAAG 1 cut(s) 958
BfuAI ACCTGC 1 cut(s) 1006
BglII AGATCT 1 cut(s) 537
BisI GCNGC 1 cut(s) 885
BlsI GCNGC 1 cut(s) 886
BmcAI AGTACT 1 cut(s) 564
Bme1390I CCNGG 1 cut(s) 21
Bme18I GGWCC 3 cut(s) 17, 62, 785
BmgT120I GGNCC 5 cut(s) 17, 62, 766, 785, 1045
BmiI GGNNCC 3 cut(s) 787, 1004, 1072
BmrFI CCNGG 1 cut(s) 21
BmsI GCATC 7 cut(s) 199, 217, 409, 624, 639, 675, 816
BpmI CTGGAG 2 cut(s) 135, 823
Bpu10I CCTNAGC 1 cut(s) 423
BpuEI CTTGAG 4 cut(s) 242, 368, 531, 779
BsaJI CCNNGG 5 cut(s) 20, 88, 606, 998, 1135
BsaWI WCCGGW 1 cut(s) 325
Bsc4I CCNNNNNNNGG 1 cut(s) 1015
Bse118I RCCGGY 1 cut(s) 1073
Bse1I ACTGG 3 cut(s) 152, 806, 1116
Bse3DI GCAATG 2 cut(s) 291, 879
BseAI TCCGGA 1 cut(s) 325
BseBI CCWGG 1 cut(s) 21
BseDI CCNNGG 5 cut(s) 20, 88, 606, 998, 1135
BseGI GGATG 3 cut(s) 562, 727, 928
BseLI CCNNNNNNNGG 1 cut(s) 1015
BseMI GCAATG 2 cut(s) 291, 879
BseMII CTCAG 1 cut(s) 414
BseNI ACTGG 3 cut(s) 152, 806, 1116
BseRI GAGGAG 1 cut(s) 1083
BseXI GCAGC 1 cut(s) 896
BseYI CCCAGC 2 cut(s) 397, 789
BsgI GTGCAG 1 cut(s) 227
BshFI GGCC 6 cut(s) 87, 128, 335, 767, 1047, 1077
BshNI GGYRCC 1 cut(s) 1002
BsiHKAI GWGCWC 4 cut(s) 29, 342, 547, 967
BsiSI CCGG 2 cut(s) 326, 1074
BslFI GGGAC 2 cut(s) 771, 984
BslI CCNNNNNNNGG 1 cut(s) 1015
BsmAI GTCTC 1 cut(s) 909
BsmFI GGGAC 2 cut(s) 771, 984
BsnI GGCC 6 cut(s) 87, 128, 335, 767, 1047, 1077
Bsp1286I GDGCHC 4 cut(s) 29, 342, 547, 967
Bsp13I TCCGGA 1 cut(s) 325
Bsp1407I TGTACA 1 cut(s) 906
Bsp143I GATC 4 cut(s) 361, 379, 537, 709
Bsp19I CCATGG 1 cut(s) 88
BspANI GGCC 6 cut(s) 87, 128, 335, 767, 1047, 1077
BspCNI CTCAG 1 cut(s) 415
BspEI TCCGGA 1 cut(s) 325
BspHI TCATGA 1 cut(s) 829
BspLI GGNNCC 3 cut(s) 787, 1004, 1072
BspMAI CTGCAG 1 cut(s) 815
BspMI ACCTGC 1 cut(s) 1006
BspPI GGATC 2 cut(s) 356, 704
BspQI GCTCTTC 1 cut(s) 34
BspT107I GGYRCC 1 cut(s) 1002
BspTI CTTAAG 1 cut(s) 958
BsrDI GCAATG 2 cut(s) 291, 879
BsrFI RCCGGY 1 cut(s) 1073
BsrGI TGTACA 1 cut(s) 906
BsrI ACTGG 3 cut(s) 152, 806, 1116
BssAI RCCGGY 1 cut(s) 1073
BssECI CCNNGG 5 cut(s) 20, 88, 606, 998, 1135
BssMI GATC 4 cut(s) 361, 379, 537, 709
BssSI CACGAG 1 cut(s) 946
BssT1I CCWWGG 2 cut(s) 88, 1135
Bst2BI CACGAG 1 cut(s) 946
Bst2UI CCWGG 1 cut(s) 21
Bst4CI ACNGT 4 cut(s) 506, 518, 911, 999
Bst6I CTCTTC 2 cut(s) 34, 922
BstAFI CTTAAG 1 cut(s) 958
BstAPI GCANNNNNTGC 1 cut(s) 227
BstAUI TGTACA 1 cut(s) 906
BstC8I GCNNGC 2 cut(s) 333, 1075
BstDEI CTNAG 2 cut(s) 95, 423
BstDSI CCRYGG 2 cut(s) 88, 998
BstF5I GGATG 3 cut(s) 562, 727, 928
BstKTI GATC 4 cut(s) 364, 382, 540, 712
BstMAI GTCTC 1 cut(s) 909
BstMBI GATC 4 cut(s) 361, 379, 537, 709
BstMWI GCNNNNNNNGC 4 cut(s) 218, 227, 337, 1079
BstNI CCWGG 1 cut(s) 21
BstNSI RCATGY 1 cut(s) 136
BstSCI CCNGG 1 cut(s) 19
BstSFI CTRYAG 1 cut(s) 811
BstV1I GCAGC 1 cut(s) 896
BstX2I RGATCY 2 cut(s) 361, 537
BstXI CCANNNNNNTGG 1 cut(s) 59
BstYI RGATCY 2 cut(s) 361, 537
BsuRI GGCC 6 cut(s) 87, 128, 335, 767, 1047, 1077
BtgI CCRYGG 2 cut(s) 88, 998
BtsCI GGATG 3 cut(s) 562, 727, 928
BtsIMutI CAGTG 2 cut(s) 916, 1109
BveI ACCTGC 1 cut(s) 1006
Cac8I GCNNGC 2 cut(s) 333, 1075
CaiI CAGNNNCTG 2 cut(s) 20, 422
CciI TCATGA 1 cut(s) 829
Cfr10I RCCGGY 1 cut(s) 1073
Cfr13I GGNCC 5 cut(s) 17, 62, 766, 785, 1045
Csp6I GTAC 4 cut(s) 563, 816, 907, 1003
CviAII CATG 8 cut(s) 89, 114, 133, 461, 773, 830, 990, 1079
CviQI GTAC 4 cut(s) 563, 816, 907, 1003
DdeI CTNAG 2 cut(s) 95, 423
DpnI GATC 4 cut(s) 363, 381, 539, 711
DpnII GATC 4 cut(s) 361, 379, 537, 709
EaeI YGGCCR 3 cut(s) 85, 333, 1075
Eam1104I CTCTTC 2 cut(s) 34, 922
EarI CTCTTC 2 cut(s) 34, 922
Ecl136II GAGCTC 1 cut(s) 340
Eco130I CCWWGG 2 cut(s) 88, 1135
Eco24I GRGCYC 1 cut(s) 342
Eco32I GATATC 1 cut(s) 471
Eco47I GGWCC 3 cut(s) 17, 62, 785
Eco53kI GAGCTC 1 cut(s) 340
EcoICRI GAGCTC 1 cut(s) 340
EcoO109I RGGNCCY 3 cut(s) 17, 766, 1045
EcoRII CCWGG 1 cut(s) 19
EcoRV GATATC 1 cut(s) 471
EcoT14I CCWWGG 2 cut(s) 88, 1135
EcoT22I ATGCAT 1 cut(s) 232
EcoT38I GRGCYC 1 cut(s) 342
ErhI CCWWGG 2 cut(s) 88, 1135
FaeI CATG 8 cut(s) 92, 117, 136, 464, 776, 833, 993, 1082
FaqI GGGAC 2 cut(s) 771, 984
FatI CATG 8 cut(s) 88, 113, 132, 460, 772, 829, 989, 1078
FauNDI CATATG 1 cut(s) 232
Fnu4HI GCNGC 1 cut(s) 885
FokI GGATG 3 cut(s) 569, 734, 935
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 1 cut(s) 885
GluI GCNGC 1 cut(s) 885
GsaI CCCAGC 2 cut(s) 401, 793
GsuI CTGGAG 2 cut(s) 135, 823
HaeIII GGCC 6 cut(s) 87, 128, 335, 767, 1047, 1077
HapII CCGG 2 cut(s) 326, 1074
Hin1II CATG 8 cut(s) 92, 117, 136, 464, 776, 833, 993, 1082
HinfI GANTC 5 cut(s) 146, 390, 457, 842, 1142
HpaII CCGG 2 cut(s) 326, 1074
HphI GGTGA 4 cut(s) 530, 632, 1030, 1114
Hpy166II GTNNAC 2 cut(s) 907, 945
Hpy188I TCNGA 4 cut(s) 206, 418, 475, 1067
Hpy188III TCNNGA 7 cut(s) 326, 407, 719, 732, 796, 830, 1034
Hpy8I GTNNAC 2 cut(s) 907, 945
HpyAV CCTTC 3 cut(s) 295, 375, 1161
HpyCH4III ACNGT 4 cut(s) 506, 518, 911, 999
HpyF10VI GCNNNNNNNGC 4 cut(s) 218, 227, 337, 1079
HpyF3I CTNAG 2 cut(s) 95, 423
Hsp92II CATG 8 cut(s) 92, 117, 136, 464, 776, 833, 993, 1082
Kpn2I TCCGGA 1 cut(s) 325
KpnI GGTACC 1 cut(s) 1006
KroI GCCGGC 1 cut(s) 1073
KroNI GCCGGC 1 cut(s) 1075
Kzo9I GATC 4 cut(s) 361, 379, 537, 709
LguI GCTCTTC 1 cut(s) 34
LmnI GCTCC 4 cut(s) 44, 328, 804, 1070
Lsp1109I GCAGC 1 cut(s) 896
LweI GCATC 7 cut(s) 199, 217, 409, 624, 639, 675, 816
MalI GATC 4 cut(s) 363, 381, 539, 711
MboI GATC 4 cut(s) 361, 379, 537, 709
MboII GAAGA 4 cut(s) 21, 679, 851, 939
MflI RGATCY 2 cut(s) 361, 537
MhlI GDGCHC 4 cut(s) 29, 342, 547, 967
MlsI TGGCCA 1 cut(s) 87
MluCI AATT 3 cut(s) 375, 693, 737
MluNI TGGCCA 1 cut(s) 87
MlyI GAGTC 2 cut(s) 399, 466
MnlI CCTC 8 cut(s) 118, 380, 418, 428, 616, 779, 1061, 1135
Mox20I TGGCCA 1 cut(s) 87
Mph1103I ATGCAT 1 cut(s) 232
MroI TCCGGA 1 cut(s) 325
MroNI GCCGGC 1 cut(s) 1073
MscI TGGCCA 1 cut(s) 87
MseI TTAA 2 cut(s) 102, 959
Msp20I TGGCCA 1 cut(s) 87
MspCI CTTAAG 1 cut(s) 958
MspI CCGG 2 cut(s) 326, 1074
MspR9I CCNGG 1 cut(s) 21
MvaI CCWGG 1 cut(s) 21
MwoI GCNNNNNNNGC 4 cut(s) 218, 227, 337, 1079
NaeI GCCGGC 1 cut(s) 1075
NcoI CCATGG 1 cut(s) 88
NdeI CATATG 1 cut(s) 232
NdeII GATC 4 cut(s) 361, 379, 537, 709
NgoMIV GCCGGC 1 cut(s) 1073
NlaIII CATG 8 cut(s) 92, 117, 136, 464, 776, 833, 993, 1082
NlaIV GGNNCC 3 cut(s) 787, 1004, 1072
NmeAIII GCCGAG 1 cut(s) 361
NsiI ATGCAT 1 cut(s) 232
NspI RCATGY 1 cut(s) 136
PagI TCATGA 1 cut(s) 829
PaqCI CACCTGC 1 cut(s) 1006
PciSI GCTCTTC 1 cut(s) 34
PdiI GCCGGC 1 cut(s) 1075
PfeI GAWTC 3 cut(s) 146, 842, 1142
PkrI GCNGC 1 cut(s) 886
PleI GAGTC 2 cut(s) 398, 465
PpsI GAGTC 2 cut(s) 398, 465
PpuMI RGGWCCY 1 cut(s) 17
PsiI TTATAA 1 cut(s) 494
Psp124BI GAGCTC 1 cut(s) 342
Psp5II RGGWCCY 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 19
PspFI CCCAGC 2 cut(s) 397, 789
PspGI CCWGG 1 cut(s) 19
PspN4I GGNNCC 3 cut(s) 787, 1004, 1072
PspPI GGNCC 5 cut(s) 17, 62, 766, 785, 1045
PspPPI RGGWCCY 1 cut(s) 17
PstI CTGCAG 1 cut(s) 815
PstNI CAGNNNCTG 2 cut(s) 20, 422
PsuI RGATCY 2 cut(s) 361, 537
RsaI GTAC 4 cut(s) 564, 817, 908, 1004
RsaNI GTAC 4 cut(s) 563, 816, 907, 1003
SacI GAGCTC 1 cut(s) 342
SapI GCTCTTC 1 cut(s) 34
SaqAI TTAA 2 cut(s) 102, 959
SatI GCNGC 1 cut(s) 885
Sau3AI GATC 4 cut(s) 361, 379, 537, 709
Sau96I GGNCC 5 cut(s) 17, 62, 766, 785, 1045
ScaI AGTACT 1 cut(s) 564
SchI GAGTC 2 cut(s) 399, 466
ScrFI CCNGG 1 cut(s) 21
SduI GDGCHC 4 cut(s) 29, 342, 547, 967
SfaNI GCATC 7 cut(s) 199, 217, 409, 624, 639, 675, 816
SfcI CTRYAG 1 cut(s) 811
SinI GGWCC 3 cut(s) 17, 62, 785
SmlI CTYRAG 5 cut(s) 257, 383, 546, 794, 958
SmoI CTYRAG 5 cut(s) 257, 383, 546, 794, 958
Sse9I AATT 3 cut(s) 375, 693, 737
SspI AATATT 1 cut(s) 825
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 1 cut(s) 19
StyI CCWWGG 2 cut(s) 88, 1135
TaaI ACNGT 4 cut(s) 506, 518, 911, 999
TaqI TCGA 2 cut(s) 647, 1159
TaqII GACCGA 1 cut(s) 598
TasI AATT 3 cut(s) 375, 693, 737
TatI WGTACW 2 cut(s) 562, 906
TfiI GAWTC 3 cut(s) 146, 842, 1142
Tru1I TTAA 2 cut(s) 102, 959
Tru9I TTAA 2 cut(s) 102, 959
TscAI CASTG 2 cut(s) 916, 1116
TseI GCWGC 1 cut(s) 884
TspDTI ATGAA 5 cut(s) 58, 159, 573, 680, 818
TspGWI ACGGA 2 cut(s) 702, 935
TspRI CASTG 2 cut(s) 916, 1116
Vha464I CTTAAG 1 cut(s) 958
VpaK11BI GGWCC 3 cut(s) 17, 62, 785
XapI RAATTY 1 cut(s) 737
XceI RCATGY 1 cut(s) 136
XcmI CCANNNNNNNNNTGG 1 cut(s) 779
ZrmI AGTACT 1 cut(s) 564
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.