FvH4_3g04620

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003577
Physical Location & Seq
Forward (+)
2651305 .. 2654139
2835 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g04620.t1

Sequence Viewer

Length: 1458 bp
ATGGAGTTTGTAGTAAGAATGATAGCCTTGTTCTTCATTCTTGCTACTGTTGTGGAGCCTCTGCCAGTTTTGGGTTCTAGGCGGACATTGCAAAAGTACTCATCATTGGAGACTGGGATTAGCACTAATAGTGATGAGGATCTTGTTACCAACTTGCCTAGCCAGCCTGCCGTAGACTTCAAGCACTATGCCGGATATGTCACCGTGAGCGAAACGAATGGACGGGCTCTCTTTTACTGGTTCTTTGAGGCTGCTACTACTCCAGATCAAAAACCTCTTGTTTTGTGGCTTAATGGAGGTCCTGGGTGCTCTTCTGTGGGATATGGAGCAACCCAAGAGATTGGTCCTTTTTTATTGGACATTGAGAGCCAAGGGTTCATATTTAATAACTACTCATGGAATAAAGAGGCAAACGTGCTATTCGTGGAATCTCCCATCGGGGTTGGCTTTTCATACACCAATACAACTTCTGATTTTCATAATCTTGGAGATAACTTCACAGCGCAAGATTCATACACTTTCCTGCATAAGTGGTTTCTCAAGTTCCCATCATTCAGAAATCGGACCTTGTACATTGCAGGCGAGAGTTATGCAGGAAAATATATTCCGGAACTAGCCGAGGTCATCTTTGATAGGAACTCAGATCCTTCTCTGCATATTGATCTCAAGGGTATCCTGATGGGTAATCCTGAAACATATGATGCTGAGGATTGGAGAGGTCTGGTGGATTATGCTTGGAGTCATGCTGTGGTTTCAGACGAAACTCACAAATTAATAAATGATAACTGTGATTTCCACAGTAATGATACTTGGAGTAACAAAAATTGTACACAAGGTGTGGATGAAGTATTTAAACAGTACAAGGAGATAGACTTGTACAGCCTTTACACCCCGACCTGCACTACAAATGATACAGCAAGTTCATATACAGCAGTTTCGAAAATGCATCTCATGACGATGAAGCGTACTTCTACAATGATTCCAAGGATTATGGGAGGCTATGATCCATGTCTTGATGGTTATTCGAAAAAGTTCTTCAACAAAGCAGATGTGCAAAAAGCCCTCCATGTCAGTGATGGTCTCCAGCTCAAGAACTGGAGCATCTGCAATGATCAAATATTTTCCAATTGGTCAGATTCACGACCATCTCTTCTTCCCACATACAAGACACTCATCGCAGCAGGACTAAGAATATGGGTGTACAGTGGAGACACGGATGGAAGAGTACCTGTGTTGTCCACAAGATATAGCTTAAGTGCCCTAGGTCTGCCTATTACAAAGACATGGAGACCTTGGTACCATCAGAAGCAGGTGAGTGGTTGGTATCAAGAGTATGAGGGGCTTACATATGCAACATTTAGAGGAGCTGGGCATGATGTTCCCTCCTTCAAACCAGCAGAGTCATTGGTTCTATTCACATCTTTTCTCCTTGGAGAATCTCCGCCATCGACTCGATAG

Protein Analysis

486

Amino Acids

54.77

Weight (kDa)

5.42

Isoelectric Point (pI)

37.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 55 - 477 1.4e-138 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1300
Acc36I ACCTGC 2 cut(s) 905, 1300
Acc65I GGTACC 1 cut(s) 1296
AccB1I GGYRCC 1 cut(s) 1296
AccI GTMKAC 1 cut(s) 174
AccIII TCCGGA 1 cut(s) 607
AciI CCGC 2 cut(s) 82, 1442
AclWI GGATC 3 cut(s) 147, 638, 998
AdeI CACNNNGTG 1 cut(s) 836
AfaI GTAC 9 cut(s) 98, 572, 829, 860, 878, 967, 1202, 1227, 1298
AfiI CCNNNNNNNGG 1 cut(s) 71
AflII CTTAAG 1 cut(s) 1252
AgsI TTSAA 3 cut(s) 181, 1039, 1390
AjnI CCWGG 1 cut(s) 301
AluBI AGCT 3 cut(s) 1087, 1251, 1367
AluI AGCT 3 cut(s) 1087, 1251, 1367
Alw21I GWGCWC 1 cut(s) 311
Alw26I GTCTC 4 cut(s) 104, 1085, 1203, 1282
AlwI GGATC 3 cut(s) 147, 638, 998
Aor13HI TCCGGA 1 cut(s) 607
ApeKI GCWGC 2 cut(s) 251, 1178
AseI ATTAAT 1 cut(s) 773
Asp700I GAANNNNTTC 1 cut(s) 1031
Asp718I GGTACC 1 cut(s) 1296
AspA2I CCTAGG 1 cut(s) 1261
AspLEI GCGC 1 cut(s) 505
AspS9I GGNCC 3 cut(s) 299, 344, 564
AsuHPI GGTGA 2 cut(s) 193, 1324
AsuII TTCGAA 2 cut(s) 938, 1025
AvaII GGWCC 3 cut(s) 299, 344, 564
AvrII CCTAGG 1 cut(s) 1261
BaeGI GKGCMC 1 cut(s) 1261
BaeI ACNNNNGTAYC 2 cut(s) 1280, 1313
BanI GGYRCC 1 cut(s) 1296
BanII GRGCYC 1 cut(s) 229
Bbv12I GWGCWC 1 cut(s) 311
BbvCI CCTCAGC 1 cut(s) 705
BbvI GCAGC 2 cut(s) 238, 1190
BccI CCATC 9 cut(s) 443, 556, 673, 1010, 1070, 1153, 1211, 1308, 1453
BceAI ACGGC 1 cut(s) 155
BcgI CGANNNNNNTGC 2 cut(s) 572, 606
BciT130I CCWGG 1 cut(s) 303
BciVI GTATCC 1 cut(s) 683
BclI TGATCA 1 cut(s) 1111
BcoDI GTCTC 4 cut(s) 104, 1085, 1203, 1282
BfaI CTAG 4 cut(s) 78, 159, 614, 1262
BfrI CTTAAG 1 cut(s) 1252
BfuAI ACCTGC 2 cut(s) 905, 1300
BfuI GTATCC 1 cut(s) 683
BisI GCNGC 2 cut(s) 252, 1179
BlnI CCTAGG 1 cut(s) 1261
BlsI GCNGC 2 cut(s) 253, 1180
BmcAI AGTACT 1 cut(s) 98
Bme1390I CCNGG 1 cut(s) 303
Bme18I GGWCC 3 cut(s) 299, 344, 564
BmgT120I GGNCC 3 cut(s) 299, 344, 564
BmiI GGNNCC 2 cut(s) 57, 1298
BmrFI CCNGG 1 cut(s) 303
BmrI ACTGGG 1 cut(s) 123
BmsI GCATC 3 cut(s) 691, 955, 1110
BmuI ACTGGG 1 cut(s) 123
BpmI CTGGAG 3 cut(s) 246, 1067, 1117
Bpu10I CCTNAGC 1 cut(s) 705
Bpu14I TTCGAA 2 cut(s) 938, 1025
BpuEI CTTGAG 3 cut(s) 524, 650, 1073
BsaBI GATNNNNATC 1 cut(s) 138
BsaI GGTCTC 2 cut(s) 1085, 1282
BsaJI CCNNGG 7 cut(s) 302, 370, 618, 983, 1261, 1292, 1429
BsaWI WCCGGW 1 cut(s) 607
Bsc4I CCNNNNNNNGG 1 cut(s) 71
Bse1I ACTGG 4 cut(s) 65, 118, 242, 1100
Bse3DI GCAATG 3 cut(s) 86, 573, 1114
Bse8I GATNNNNATC 1 cut(s) 138
BseAI TCCGGA 1 cut(s) 607
BseBI CCWGG 1 cut(s) 303
BseDI CCNNGG 7 cut(s) 302, 370, 618, 983, 1261, 1292, 1429
BseGI GGATG 2 cut(s) 847, 1222
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 1 cut(s) 71
BseMI GCAATG 3 cut(s) 86, 573, 1114
BseMII CTCAG 2 cut(s) 654, 696
BseNI ACTGG 4 cut(s) 65, 118, 242, 1100
BseRI GAGGAG 1 cut(s) 1377
BseSI GKGCMC 1 cut(s) 1261
BseXI GCAGC 2 cut(s) 238, 1190
BseYI CCCAGC 1 cut(s) 1367
BsgI GTGCAG 1 cut(s) 883
BshNI GGYRCC 1 cut(s) 1296
BsiHKAI GWGCWC 1 cut(s) 311
BsiSI CCGG 2 cut(s) 192, 608
BslI CCNNNNNNNGG 1 cut(s) 71
BsmAI GTCTC 4 cut(s) 104, 1085, 1203, 1282
Bso31I GGTCTC 2 cut(s) 1085, 1282
Bsp119I TTCGAA 2 cut(s) 938, 1025
Bsp1286I GDGCHC 3 cut(s) 229, 311, 1261
Bsp13I TCCGGA 1 cut(s) 607
Bsp1407I TGTACA 4 cut(s) 570, 827, 876, 1200
Bsp143I GATC 6 cut(s) 139, 265, 643, 661, 1003, 1111
BspACI CCGC 2 cut(s) 82, 1442
BspCNI CTCAG 2 cut(s) 653, 697
BspEI TCCGGA 1 cut(s) 607
BspHI TCATGA 1 cut(s) 951
BspLI GGNNCC 2 cut(s) 57, 1298
BspMI ACCTGC 2 cut(s) 905, 1300
BspPI GGATC 3 cut(s) 147, 638, 998
BspQI GCTCTTC 1 cut(s) 316
BspT104I TTCGAA 2 cut(s) 938, 1025
BspT107I GGYRCC 1 cut(s) 1296
BspTI CTTAAG 1 cut(s) 1252
BspTNI GGTCTC 2 cut(s) 1085, 1282
BsrDI GCAATG 3 cut(s) 86, 573, 1114
BsrGI TGTACA 4 cut(s) 570, 827, 876, 1200
BsrI ACTGG 4 cut(s) 65, 118, 242, 1100
BssECI CCNNGG 7 cut(s) 302, 370, 618, 983, 1261, 1292, 1429
BssMI GATC 6 cut(s) 139, 265, 643, 661, 1003, 1111
BssT1I CCWWGG 5 cut(s) 370, 983, 1261, 1292, 1429
Bst2UI CCWGG 1 cut(s) 303
Bst4CI ACNGT 6 cut(s) 49, 205, 788, 800, 858, 1205
Bst6I CTCTTC 3 cut(s) 316, 1155, 1216
BstAFI CTTAAG 1 cut(s) 1252
BstAUI TGTACA 4 cut(s) 570, 827, 876, 1200
BstBI TTCGAA 2 cut(s) 938, 1025
BstC8I GCNNGC 3 cut(s) 164, 168, 580
BstDEI CTNAG 3 cut(s) 640, 705, 1187
BstF5I GGATG 2 cut(s) 847, 1222
BstHHI GCGC 1 cut(s) 505
BstKTI GATC 6 cut(s) 142, 268, 646, 664, 1006, 1114
BstMAI GTCTC 4 cut(s) 104, 1085, 1203, 1282
BstMBI GATC 6 cut(s) 139, 265, 643, 661, 1003, 1111
BstMWI GCNNNNNNNGC 2 cut(s) 88, 163
BstNI CCWGG 1 cut(s) 303
BstSCI CCNGG 1 cut(s) 301
BstSLI GKGCMC 1 cut(s) 1261
BstV1I GCAGC 2 cut(s) 238, 1190
BstX2I RGATCY 2 cut(s) 139, 643
BstXI CCANNNNNNTGG 1 cut(s) 341
BstYI RGATCY 2 cut(s) 139, 643
BsuI GTATCC 1 cut(s) 683
BtgZI GCGATG 1 cut(s) 1159
BtsCI GGATG 2 cut(s) 847, 1222
BtsIMutI CAGTG 2 cut(s) 1078, 1210
BveI ACCTGC 2 cut(s) 905, 1300
Cac8I GCNNGC 3 cut(s) 164, 168, 580
CciI TCATGA 1 cut(s) 951
CfoI GCGC 1 cut(s) 505
Cfr13I GGNCC 3 cut(s) 299, 344, 564
Csp6I GTAC 9 cut(s) 97, 571, 828, 859, 877, 966, 1201, 1226, 1297
CviAII CATG 7 cut(s) 396, 743, 952, 1008, 1067, 1284, 1373
CviQI GTAC 9 cut(s) 97, 571, 828, 859, 877, 966, 1201, 1226, 1297
DdeI CTNAG 3 cut(s) 640, 705, 1187
DpnI GATC 6 cut(s) 141, 267, 645, 663, 1005, 1113
DpnII GATC 6 cut(s) 139, 265, 643, 661, 1003, 1111
DraI TTTAAA 1 cut(s) 853
DraIII CACNNNGTG 1 cut(s) 836
Eam1104I CTCTTC 3 cut(s) 316, 1155, 1216
EarI CTCTTC 3 cut(s) 316, 1155, 1216
EciI GGCGGA 2 cut(s) 97, 1431
Eco130I CCWWGG 5 cut(s) 370, 983, 1261, 1292, 1429
Eco24I GRGCYC 1 cut(s) 229
Eco31I GGTCTC 2 cut(s) 1085, 1282
Eco47I GGWCC 3 cut(s) 299, 344, 564
EcoO109I RGGNCCY 1 cut(s) 299
EcoRII CCWGG 1 cut(s) 301
EcoT14I CCWWGG 5 cut(s) 370, 983, 1261, 1292, 1429
EcoT22I ATGCAT 1 cut(s) 948
EcoT38I GRGCYC 1 cut(s) 229
ErhI CCWWGG 5 cut(s) 370, 983, 1261, 1292, 1429
FaeI CATG 7 cut(s) 399, 746, 955, 1011, 1070, 1287, 1376
FatI CATG 7 cut(s) 395, 742, 951, 1007, 1066, 1283, 1372
FauNDI CATATG 2 cut(s) 697, 1348
FbaI TGATCA 1 cut(s) 1111
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 2 cut(s) 252, 1179
FokI GGATG 2 cut(s) 854, 1229
FriOI GRGCYC 1 cut(s) 229
Fsp4HI GCNGC 2 cut(s) 252, 1179
FspBI CTAG 4 cut(s) 78, 159, 614, 1262
GlaI GCGC 1 cut(s) 504
GluI GCNGC 2 cut(s) 252, 1179
GsaI CCCAGC 1 cut(s) 1371
GsuI CTGGAG 3 cut(s) 246, 1067, 1117
HapII CCGG 2 cut(s) 192, 608
HhaI GCGC 1 cut(s) 505
Hin1II CATG 7 cut(s) 399, 746, 955, 1011, 1070, 1287, 1376
Hin6I GCGC 1 cut(s) 503
HinP1I GCGC 1 cut(s) 503
HinfI GANTC 8 cut(s) 428, 509, 739, 979, 1136, 1400, 1436, 1450
HpaII CCGG 2 cut(s) 192, 608
HphI GGTGA 2 cut(s) 193, 1324
Hpy166II GTNNAC 4 cut(s) 175, 830, 1201, 1239
Hpy188I TCNGA 7 cut(s) 472, 557, 564, 643, 757, 1135, 1305
Hpy188III TCNNGA 9 cut(s) 263, 608, 676, 689, 952, 1013, 1090, 1140, 1328
Hpy8I GTNNAC 4 cut(s) 175, 830, 1201, 1239
HpyAV CCTTC 2 cut(s) 657, 1396
HpyCH4III ACNGT 6 cut(s) 49, 205, 788, 800, 858, 1205
HpyCH4IV ACGT 1 cut(s) 414
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 163
HpyF3I CTNAG 3 cut(s) 640, 705, 1187
HpySE526I ACGT 1 cut(s) 414
Hsp92II CATG 7 cut(s) 399, 746, 955, 1011, 1070, 1287, 1376
HspAI GCGC 1 cut(s) 503
Kpn2I TCCGGA 1 cut(s) 607
KpnI GGTACC 1 cut(s) 1300
Ksp22I TGATCA 1 cut(s) 1111
Kzo9I GATC 6 cut(s) 139, 265, 643, 661, 1003, 1111
LguI GCTCTTC 1 cut(s) 316
LmnI GCTCC 4 cut(s) 55, 326, 1098, 1364
Lsp1109I GCAGC 2 cut(s) 238, 1190
LweI GCATC 3 cut(s) 691, 955, 1110
MaeI CTAG 4 cut(s) 78, 159, 614, 1262
MaeII ACGT 1 cut(s) 414
MaeIII GTNAC 3 cut(s) 145, 199, 815
MalI GATC 6 cut(s) 141, 267, 645, 663, 1005, 1113
MboI GATC 6 cut(s) 139, 265, 643, 661, 1003, 1111
MboII GAAGA 6 cut(s) 25, 303, 1027, 1142, 1145, 1233
MfeI CAATTG 1 cut(s) 1126
MflI RGATCY 2 cut(s) 139, 643
MhlI GDGCHC 3 cut(s) 229, 311, 1261
MluCI AATT 3 cut(s) 770, 823, 1126
MlyI GAGTC 3 cut(s) 748, 1409, 1444
Mph1103I ATGCAT 1 cut(s) 948
MroI TCCGGA 1 cut(s) 607
MroXI GAANNNNTTC 1 cut(s) 1031
MseI TTAA 5 cut(s) 291, 384, 773, 852, 1253
MslI CAYNNNNRTG 3 cut(s) 801, 956, 1071
MspCI CTTAAG 1 cut(s) 1252
MspI CCGG 2 cut(s) 192, 608
MspR9I CCNGG 1 cut(s) 303
MunI CAATTG 1 cut(s) 1126
MvaI CCWGG 1 cut(s) 303
MwoI GCNNNNNNNGC 2 cut(s) 88, 163
NdeI CATATG 2 cut(s) 697, 1348
NdeII GATC 6 cut(s) 139, 265, 643, 661, 1003, 1111
NlaIII CATG 7 cut(s) 399, 746, 955, 1011, 1070, 1287, 1376
NlaIV GGNNCC 2 cut(s) 57, 1298
NmeAIII GCCGAG 1 cut(s) 643
NmuCI GTSAC 1 cut(s) 199
NsiI ATGCAT 1 cut(s) 948
NspV TTCGAA 2 cut(s) 938, 1025
PagI TCATGA 1 cut(s) 951
PaqCI CACCTGC 1 cut(s) 1300
PciSI GCTCTTC 1 cut(s) 316
PcsI WCGNNNNNNNCGW 1 cut(s) 420
PdmI GAANNNNTTC 1 cut(s) 1031
PfeI GAWTC 5 cut(s) 428, 509, 979, 1136, 1436
PkrI GCNGC 2 cut(s) 253, 1180
PleI GAGTC 3 cut(s) 747, 1408, 1444
PpsI GAGTC 3 cut(s) 747, 1408, 1444
PpuMI RGGWCCY 1 cut(s) 299
PshBI ATTAAT 1 cut(s) 773
Psp5II RGGWCCY 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 301
PspFI CCCAGC 1 cut(s) 1367
PspGI CCWGG 1 cut(s) 301
PspN4I GGNNCC 2 cut(s) 57, 1298
PspPI GGNCC 3 cut(s) 299, 344, 564
PspPPI RGGWCCY 1 cut(s) 299
PsuI RGATCY 2 cut(s) 139, 643
RsaI GTAC 9 cut(s) 98, 572, 829, 860, 878, 967, 1202, 1227, 1298
RsaNI GTAC 9 cut(s) 97, 571, 828, 859, 877, 966, 1201, 1226, 1297
RseI CAYNNNNRTG 3 cut(s) 801, 956, 1071
SapI GCTCTTC 1 cut(s) 316
SaqAI TTAA 5 cut(s) 291, 384, 773, 852, 1253
SatI GCNGC 2 cut(s) 252, 1179
Sau3AI GATC 6 cut(s) 139, 265, 643, 661, 1003, 1111
Sau96I GGNCC 3 cut(s) 299, 344, 564
ScaI AGTACT 1 cut(s) 98
SchI GAGTC 3 cut(s) 748, 1409, 1444
ScrFI CCNGG 1 cut(s) 303
SduI GDGCHC 3 cut(s) 229, 311, 1261
SfaNI GCATC 3 cut(s) 691, 955, 1110
SfuI TTCGAA 2 cut(s) 938, 1025
SinI GGWCC 3 cut(s) 299, 344, 564
SmiMI CAYNNNNRTG 3 cut(s) 801, 956, 1071
SmlI CTYRAG 4 cut(s) 539, 665, 1088, 1252
SmoI CTYRAG 4 cut(s) 539, 665, 1088, 1252
Sse9I AATT 3 cut(s) 770, 823, 1126
SsiI CCGC 2 cut(s) 82, 1442
SspI AATATT 1 cut(s) 1119
SspMI CTAG 4 cut(s) 78, 159, 614, 1262
StyD4I CCNGG 1 cut(s) 301
StyI CCWWGG 5 cut(s) 370, 983, 1261, 1292, 1429
TaaI ACNGT 6 cut(s) 49, 205, 788, 800, 858, 1205
TaiI ACGT 1 cut(s) 417
TaqI TCGA 4 cut(s) 938, 1025, 1448, 1453
TasI AATT 3 cut(s) 770, 823, 1126
TatI WGTACW 6 cut(s) 96, 570, 827, 858, 876, 1200
TfiI GAWTC 5 cut(s) 428, 509, 979, 1136, 1436
Tru1I TTAA 5 cut(s) 291, 384, 773, 852, 1253
Tru9I TTAA 5 cut(s) 291, 384, 773, 852, 1253
TscAI CASTG 2 cut(s) 1078, 1210
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 2 cut(s) 251, 1178
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 8 cut(s) 25, 367, 441, 467, 501, 858, 912, 974
TspGWI ACGGA 1 cut(s) 1229
TspRI CASTG 2 cut(s) 1078, 1210
Vha464I CTTAAG 1 cut(s) 1252
VpaK11BI GGWCC 3 cut(s) 299, 344, 564
VspI ATTAAT 1 cut(s) 773
XcmI CCANNNNNNNNNTGG 1 cut(s) 1073
XmaJI CCTAGG 1 cut(s) 1261
XmiI GTMKAC 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 1031
XspI CTAG 4 cut(s) 78, 159, 614, 1262
ZrmI AGTACT 1 cut(s) 98
Zsp2I ATGCAT 1 cut(s) 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.