AT1G61280

PIG-P (InterPro IPR013717), Phosphatidylinositol N-acetylglucosaminyltransferase, GPI19 PIG-P subunit (InterPro IPR016542)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004032
Physical Location & Seq
Forward (+)
22603586 .. 22605008
1423 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G61280.2

Sequence Viewer

Length: 414 bp
ATGTTAAGCTTGAATCAAGAAGTACACGGACCAAAAACTTCCGAGGTTTATGGTTTTGTAGGATCTATTTCGATTGTTGTTGCCACTGTGATCTTCTTAATTTGGGGATATGTACCAGATAAGTTCTTAGAGTCCATAGGCATTTATTACTACCCGAGCAAGTACTGGGCCATGGCGATGCCGATGTATTCGATGGTGACTCTGTTAGTAGCTTTGGTGTTTTACATTGGTCTCAACTTCATGTCTACTTCAAAACCAACCTCTCTCAATACTTTGTTTGATGACTACAGTAGAGAAGATGTGAATTTTCTTCCTTTGATGAAGAATGGGGAAGACAGGCCAATAGATCCAATCTCTGACATTGATATTACAAGAATCAACGATCTTATGTTCGATTCACATCTTGCCAAGTGA

Protein Analysis

137

Amino Acids

15.61

Weight (kDa)

4.64

Isoelectric Point (pI)

25.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIG-P PF08510 13 - 131 2.5e-38 PIG-P
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 245
AclWI GGATC 2 cut(s) 70, 341
AcsI RAATTY 1 cut(s) 304
AfaI GTAC 3 cut(s) 24, 114, 164
AgsI TTSAA 2 cut(s) 13, 252
AluBI AGCT 2 cut(s) 9, 212
AluI AGCT 2 cut(s) 9, 212
Alw26I GTCTC 1 cut(s) 236
AlwI GGATC 2 cut(s) 70, 341
Ama87I CYCGRG 1 cut(s) 154
AoxI GGCC 2 cut(s) 168, 338
ApoI RAATTY 1 cut(s) 304
AspS9I GGNCC 2 cut(s) 29, 168
AsuHPI GGTGA 1 cut(s) 208
AvaI CYCGRG 1 cut(s) 154
AvaII GGWCC 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 339
BccI CCATC 1 cut(s) 187
BcoDI GTCTC 1 cut(s) 236
BfmI CTRYAG 1 cut(s) 286
BmcAI AGTACT 1 cut(s) 164
Bme18I GGWCC 1 cut(s) 29
BmeT110I CYCGRG 1 cut(s) 154
BmgT120I GGNCC 2 cut(s) 29, 168
BmrI ACTGGG 1 cut(s) 175
BmsI GCATC 1 cut(s) 168
BmuI ACTGGG 1 cut(s) 175
BpiI GAAGAC 1 cut(s) 339
BsaBI GATNNNNATC 1 cut(s) 399
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 2 cut(s) 42, 171
Bse1I ACTGG 1 cut(s) 170
Bse8I GATNNNNATC 1 cut(s) 399
BseDI CCNNGG 2 cut(s) 42, 171
BseJI GATNNNNATC 1 cut(s) 399
BseNI ACTGG 1 cut(s) 170
BshFI GGCC 2 cut(s) 170, 340
BsiHKCI CYCGRG 1 cut(s) 154
BsmAI GTCTC 1 cut(s) 236
BsnI GGCC 2 cut(s) 170, 340
Bso31I GGTCTC 1 cut(s) 236
BsoBI CYCGRG 1 cut(s) 154
Bsp143I GATC 4 cut(s) 62, 90, 346, 382
Bsp19I CCATGG 1 cut(s) 171
BspANI GGCC 2 cut(s) 170, 340
BspPI GGATC 2 cut(s) 70, 341
BspTNI GGTCTC 1 cut(s) 236
BsrI ACTGG 1 cut(s) 170
BssECI CCNNGG 2 cut(s) 42, 171
BssMI GATC 4 cut(s) 62, 90, 346, 382
BssT1I CCWWGG 1 cut(s) 171
Bst4CI ACNGT 2 cut(s) 88, 290
BstDEI CTNAG 1 cut(s) 127
BstDSI CCRYGG 1 cut(s) 171
BstKTI GATC 4 cut(s) 65, 93, 349, 385
BstMAI GTCTC 1 cut(s) 236
BstMBI GATC 4 cut(s) 62, 90, 346, 382
BstSFI CTRYAG 1 cut(s) 286
BstV2I GAAGAC 1 cut(s) 339
BstX2I RGATCY 2 cut(s) 62, 346
BstYI RGATCY 2 cut(s) 62, 346
BsuRI GGCC 2 cut(s) 170, 340
BtgI CCRYGG 1 cut(s) 171
BtgZI GCGATG 1 cut(s) 191
BtsIMutI CAGTG 1 cut(s) 84
Cfr13I GGNCC 2 cut(s) 29, 168
Csp6I GTAC 3 cut(s) 23, 113, 163
CviAII CATG 2 cut(s) 172, 241
CviJI RGCY 4 cut(s) 9, 170, 212, 340
CviKI_1 RGCY 4 cut(s) 9, 170, 212, 340
CviQI GTAC 3 cut(s) 23, 113, 163
DdeI CTNAG 1 cut(s) 127
DpnI GATC 4 cut(s) 64, 92, 348, 384
DpnII GATC 4 cut(s) 62, 90, 346, 382
Eco130I CCWWGG 1 cut(s) 171
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 1 cut(s) 29
Eco88I CYCGRG 1 cut(s) 154
EcoT14I CCWWGG 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 2 cut(s) 175, 244
FaiI YATR 6 cut(s) 51, 111, 137, 173, 242, 389
FatI CATG 2 cut(s) 171, 240
FblI GTMKAC 1 cut(s) 245
HaeIII GGCC 2 cut(s) 170, 340
Hin1II CATG 2 cut(s) 175, 244
HindIII AAGCTT 1 cut(s) 7
HinfI GANTC 5 cut(s) 13, 131, 199, 375, 395
HphI GGTGA 1 cut(s) 208
Hpy166II GTNNAC 2 cut(s) 25, 246
Hpy188I TCNGA 2 cut(s) 43, 358
Hpy188III TCNNGA 1 cut(s) 17
Hpy8I GTNNAC 2 cut(s) 25, 246
HpyCH4III ACNGT 2 cut(s) 88, 290
HpyF3I CTNAG 1 cut(s) 127
Hsp92II CATG 2 cut(s) 175, 244
Kzo9I GATC 4 cut(s) 62, 90, 346, 382
LpnPI CCDG 3 cut(s) 129, 151, 322
LweI GCATC 1 cut(s) 168
MaeIII GTNAC 1 cut(s) 196
MalI GATC 4 cut(s) 64, 92, 348, 384
MboI GATC 4 cut(s) 62, 90, 346, 382
MboII GAAGA 5 cut(s) 85, 302, 308, 334, 344
MflI RGATCY 2 cut(s) 62, 346
MluCI AATT 2 cut(s) 99, 304
MlyI GAGTC 2 cut(s) 140, 193
MnlI CCTC 2 cut(s) 37, 271
MseI TTAA 2 cut(s) 5, 98
MslI CAYNNNNRTG 1 cut(s) 176
NcoI CCATGG 1 cut(s) 171
NdeII GATC 4 cut(s) 62, 90, 346, 382
NlaIII CATG 2 cut(s) 175, 244
NmuCI GTSAC 1 cut(s) 196
PfeI GAWTC 3 cut(s) 13, 375, 395
PleI GAGTC 2 cut(s) 139, 193
PpsI GAGTC 2 cut(s) 139, 193
PspPI GGNCC 2 cut(s) 29, 168
PsuI RGATCY 2 cut(s) 62, 346
RsaI GTAC 3 cut(s) 24, 114, 164
RsaNI GTAC 3 cut(s) 23, 113, 163
RseI CAYNNNNRTG 1 cut(s) 176
SaqAI TTAA 2 cut(s) 5, 98
Sau3AI GATC 4 cut(s) 62, 90, 346, 382
Sau96I GGNCC 2 cut(s) 29, 168
ScaI AGTACT 1 cut(s) 164
SchI GAGTC 2 cut(s) 140, 193
SetI ASST 4 cut(s) 11, 48, 214, 263
SfaNI GCATC 1 cut(s) 168
SfcI CTRYAG 1 cut(s) 286
SinI GGWCC 1 cut(s) 29
SmiMI CAYNNNNRTG 1 cut(s) 176
Sse9I AATT 2 cut(s) 99, 304
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 2 cut(s) 88, 290
TaqI TCGA 3 cut(s) 71, 191, 393
TasI AATT 2 cut(s) 99, 304
TatI WGTACW 2 cut(s) 22, 162
TfiI GAWTC 3 cut(s) 13, 375, 395
Tru1I TTAA 2 cut(s) 5, 98
Tru9I TTAA 2 cut(s) 5, 98
TscAI CASTG 1 cut(s) 91
TseFI GTSAC 1 cut(s) 196
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 2 cut(s) 229, 335
TspGWI ACGGA 1 cut(s) 42
TspRI CASTG 1 cut(s) 91
VpaK11BI GGWCC 1 cut(s) 29
XapI RAATTY 1 cut(s) 304
XmiI GTMKAC 1 cut(s) 245
ZrmI AGTACT 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.