AT2G39445

PIG-P (InterPro IPR013717), Phosphatidylinositol N-acetylglucosaminyltransferase, GPI19 PIG-P subunit (InterPro IPR016542)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004032
Physical Location & Seq
Reverse (-)
16469779 .. 16471274
1496 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G39445.3

Sequence Viewer

Length: 546 bp
ATGTCTCCGGCTAAAGTCTCTTCCTTTGACCAAAACATTGAAGAAGCTCATTCAGTGAGTAGTCCAAGACGAGTTTTAAGCTTCTCGAAGAGAAATAAGCAAAAGCCTGGTTTCCAAGATACAGACAGTAGAAGGTCATCTTCATTTAGAGCATCAGAAGTGCATGGACCTAAACCCTCAGAGGTGTACGGTTTCGTAGGATCGATATCGACTGTTGTTGCCACAGTCATCTTCTTGGTATGGGCATATGTTCCAGATAAGTTGTTGGAGTCTATAGGAATCCATTACTACCCTAGTAGGTATTGGGTATTGGCTATGCCAACGTATTTGATGGTGACTCTGATGTTAGCTTTGGCTTTTTACATTGGTCTCAACTTCATCGCCACTCCACCCCCAACCTCTCTCAATACCTTGTTTGATGAGTACAGTAGAGAACCTGGTGAATTTGCTGCTGGGATGGAGGAAGGTGAAGACAGGCCTATAGATCCAATCTCTGACATTGATATTACAAGAATAAATGATCTTATGTTCAATTCAAGTGATTAA

Protein Analysis

181

Amino Acids

20.28

Weight (kDa)

5.07

Isoelectric Point (pI)

60.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIG-P PF08510 60 - 177 3.6e-40 PIG-P
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 208, 479
AcsI RAATTY 1 cut(s) 443
AfaI GTAC 2 cut(s) 188, 425
AgsI TTSAA 3 cut(s) 41, 532, 537
AjnI CCWGG 2 cut(s) 106, 436
AluBI AGCT 3 cut(s) 47, 81, 350
AluI AGCT 3 cut(s) 47, 81, 350
Alw26I GTCTC 3 cut(s) 9, 22, 374
AlwI GGATC 2 cut(s) 208, 479
AoxI GGCC 1 cut(s) 476
ApeKI GCWGC 1 cut(s) 449
ApoI RAATTY 1 cut(s) 443
AspS9I GGNCC 1 cut(s) 167
AsuHPI GGTGA 3 cut(s) 346, 452, 479
AvaII GGWCC 1 cut(s) 167
BbsI GAAGAC 1 cut(s) 477
BbvI GCAGC 1 cut(s) 436
BccI CCATC 2 cut(s) 325, 451
BciT130I CCWGG 2 cut(s) 108, 438
BcoDI GTCTC 3 cut(s) 9, 22, 374
BfaI CTAG 1 cut(s) 294
BfmI CTRYAG 2 cut(s) 273, 480
BisI GCNGC 1 cut(s) 450
BlsI GCNGC 1 cut(s) 451
Bme1390I CCNGG 2 cut(s) 108, 438
Bme18I GGWCC 1 cut(s) 167
BmgT120I GGNCC 1 cut(s) 167
BmrFI CCNGG 2 cut(s) 108, 438
BmsI GCATC 1 cut(s) 161
BpiI GAAGAC 1 cut(s) 477
Bsa29I ATCGAT 1 cut(s) 203
BsaBI GATNNNNATC 1 cut(s) 205
BsaI GGTCTC 1 cut(s) 374
Bse8I GATNNNNATC 1 cut(s) 205
BseBI CCWGG 2 cut(s) 108, 438
BseCI ATCGAT 1 cut(s) 203
BseGI GGATG 1 cut(s) 462
BseJI GATNNNNATC 1 cut(s) 205
BseMII CTCAG 1 cut(s) 192
BseXI GCAGC 1 cut(s) 436
BseYI CCCAGC 1 cut(s) 452
BshFI GGCC 1 cut(s) 478
BshVI ATCGAT 1 cut(s) 203
BsiSI CCGG 1 cut(s) 8
BsmAI GTCTC 3 cut(s) 9, 22, 374
BsnI GGCC 1 cut(s) 478
Bso31I GGTCTC 1 cut(s) 374
Bsp143I GATC 3 cut(s) 200, 484, 520
BspANI GGCC 1 cut(s) 478
BspCNI CTCAG 1 cut(s) 191
BspDI ATCGAT 1 cut(s) 203
BspPI GGATC 2 cut(s) 208, 479
BspTNI GGTCTC 1 cut(s) 374
BssMI GATC 3 cut(s) 200, 484, 520
Bst2UI CCWGG 2 cut(s) 108, 438
Bst4CI ACNGT 5 cut(s) 128, 191, 214, 226, 428
Bst6I CTCTTC 2 cut(s) 25, 83
BstDEI CTNAG 1 cut(s) 178
BstF5I GGATG 1 cut(s) 462
BstKTI GATC 3 cut(s) 203, 487, 523
BstMAI GTCTC 3 cut(s) 9, 22, 374
BstMBI GATC 3 cut(s) 200, 484, 520
BstNI CCWGG 2 cut(s) 108, 438
BstSCI CCNGG 2 cut(s) 106, 436
BstSFI CTRYAG 2 cut(s) 273, 480
BstV1I GCAGC 1 cut(s) 436
BstV2I GAAGAC 1 cut(s) 477
BstX2I RGATCY 1 cut(s) 484
BstYI RGATCY 1 cut(s) 484
Bsu15I ATCGAT 1 cut(s) 203
BsuRI GGCC 1 cut(s) 478
BsuTUI ATCGAT 1 cut(s) 203
BtgZI GCGATG 1 cut(s) 364
BtsCI GGATG 1 cut(s) 462
BtsIMutI CAGTG 1 cut(s) 60
Cfr13I GGNCC 1 cut(s) 167
ClaI ATCGAT 1 cut(s) 203
CsiI ACCWGGT 1 cut(s) 436
Csp6I GTAC 2 cut(s) 187, 424
CviAII CATG 1 cut(s) 164
CviJI RGCY 8 cut(s) 11, 47, 81, 106, 314, 350, 356, 478
CviKI_1 RGCY 8 cut(s) 11, 47, 81, 106, 314, 350, 356, 478
CviQI GTAC 2 cut(s) 187, 424
DdeI CTNAG 1 cut(s) 178
DpnI GATC 3 cut(s) 202, 486, 522
DpnII GATC 3 cut(s) 200, 484, 520
Eam1104I CTCTTC 2 cut(s) 25, 83
EarI CTCTTC 2 cut(s) 25, 83
Eco147I AGGCCT 1 cut(s) 478
Eco31I GGTCTC 1 cut(s) 374
Eco32I GATATC 1 cut(s) 207
Eco47I GGWCC 1 cut(s) 167
EcoRII CCWGG 2 cut(s) 106, 436
EcoRV GATATC 1 cut(s) 207
FaeI CATG 1 cut(s) 167
FaiI YATR 8 cut(s) 165, 241, 247, 249, 275, 317, 482, 527
FalI AAGNNNNNCTT 2 cut(s) 124, 156
FatI CATG 1 cut(s) 163
FauNDI CATATG 1 cut(s) 247
Fnu4HI GCNGC 1 cut(s) 450
FokI GGATG 1 cut(s) 469
Fsp4HI GCNGC 1 cut(s) 450
FspBI CTAG 1 cut(s) 294
GluI GCNGC 1 cut(s) 450
GsaI CCCAGC 1 cut(s) 456
HaeIII GGCC 1 cut(s) 478
HapII CCGG 1 cut(s) 8
Hin1II CATG 1 cut(s) 167
HindIII AAGCTT 1 cut(s) 79
HinfI GANTC 3 cut(s) 269, 279, 337
HpaII CCGG 1 cut(s) 8
HphI GGTGA 3 cut(s) 346, 452, 479
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 4 cut(s) 157, 181, 342, 496
Hpy188III TCNNGA 2 cut(s) 85, 254
Hpy8I GTNNAC 1 cut(s) 187
HpyAV CCTTC 2 cut(s) 126, 458
HpyCH4III ACNGT 5 cut(s) 128, 191, 214, 226, 428
HpyCH4IV ACGT 1 cut(s) 323
HpyCH4V TGCA 1 cut(s) 163
HpyF3I CTNAG 1 cut(s) 178
HpySE526I ACGT 1 cut(s) 323
Hsp92II CATG 1 cut(s) 167
Kzo9I GATC 3 cut(s) 200, 484, 520
LpnPI CCDG 8 cut(s) 21, 93, 120, 267, 423, 438, 450, 460
Lsp1109I GCAGC 1 cut(s) 436
LweI GCATC 1 cut(s) 161
MabI ACCWGGT 1 cut(s) 436
MaeI CTAG 1 cut(s) 294
MaeII ACGT 1 cut(s) 323
MaeIII GTNAC 1 cut(s) 334
MalI GATC 3 cut(s) 202, 486, 522
MboI GATC 3 cut(s) 200, 484, 520
MboII GAAGA 6 cut(s) 12, 53, 100, 132, 223, 482
MflI RGATCY 1 cut(s) 484
MluCI AATT 2 cut(s) 443, 532
MlyI GAGTC 2 cut(s) 278, 331
MmeI TCCRAC 1 cut(s) 246
MnlI CCTC 4 cut(s) 175, 187, 409, 454
MseI TTAA 2 cut(s) 77, 544
MspI CCGG 1 cut(s) 8
MspR9I CCNGG 2 cut(s) 108, 438
MvaI CCWGG 2 cut(s) 108, 438
NdeI CATATG 1 cut(s) 247
NdeII GATC 3 cut(s) 200, 484, 520
NlaIII CATG 1 cut(s) 167
NmuCI GTSAC 1 cut(s) 334
PceI AGGCCT 1 cut(s) 478
PfeI GAWTC 1 cut(s) 279
PkrI GCNGC 1 cut(s) 451
PleI GAGTC 2 cut(s) 277, 331
PpsI GAGTC 2 cut(s) 277, 331
Psp6I CCWGG 2 cut(s) 106, 436
PspFI CCCAGC 1 cut(s) 452
PspGI CCWGG 2 cut(s) 106, 436
PspPI GGNCC 1 cut(s) 167
PsuI RGATCY 1 cut(s) 484
RsaI GTAC 2 cut(s) 188, 425
RsaNI GTAC 2 cut(s) 187, 424
SaqAI TTAA 2 cut(s) 77, 544
SatI GCNGC 1 cut(s) 450
Sau3AI GATC 3 cut(s) 200, 484, 520
Sau96I GGNCC 1 cut(s) 167
SchI GAGTC 2 cut(s) 278, 331
ScrFI CCNGG 2 cut(s) 108, 438
SexAI ACCWGGT 1 cut(s) 436
SfaNI GCATC 1 cut(s) 161
SfcI CTRYAG 2 cut(s) 273, 480
SinI GGWCC 1 cut(s) 167
Sse9I AATT 2 cut(s) 443, 532
SseBI AGGCCT 1 cut(s) 478
SspMI CTAG 1 cut(s) 294
StuI AGGCCT 1 cut(s) 478
StyD4I CCNGG 2 cut(s) 106, 436
TaaI ACNGT 5 cut(s) 128, 191, 214, 226, 428
TaiI ACGT 1 cut(s) 326
TaqI TCGA 3 cut(s) 86, 203, 209
TasI AATT 2 cut(s) 443, 532
TatI WGTACW 1 cut(s) 423
TfiI GAWTC 1 cut(s) 279
Tru1I TTAA 2 cut(s) 77, 544
Tru9I TTAA 2 cut(s) 77, 544
TscAI CASTG 1 cut(s) 60
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 1 cut(s) 449
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 2 cut(s) 132, 367
TspRI CASTG 1 cut(s) 60
VpaK11BI GGWCC 1 cut(s) 167
XapI RAATTY 1 cut(s) 443
XspI CTAG 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.