AT1G62870

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001384
Physical Location & Seq
Reverse (-)
23283912 .. 23286797
2886 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G62870.1

Sequence Viewer

Length: 2289 bp
ATGGCGACGACGACAAACGCAACAACAACGGCGACACAACAGCAAACGCAAACGCAATCACCAACGCAACAACCGTCGGCGGAAGAATTAGCGACAAAGGCGTTACAAAAGCGTTACGAAGGATTAATGATGGTAAGAACAAAAGCAGTTAAAGGCAAAGGCGCGTGGTATTGGAGTCACCTTGAACCAATCTTACTACACAACACCGACACTGGTTTCCCTAAAGCCGTTAAACTCCGATGTTCTTTATGCGACGCCGTTTTCTCAGCTTCTAATCCTTCCCGAACTGCTTCTGAGCATCTTAAACGTGGAACTTGTCCAAATTTCAACTCTTTACCTAAACCCATCTCCACAATCTCTCCTTCTCCTCCTCCTCCTCCGTCTTCTTCTCACCGGAAACGTAATTCCTCCGCCGTGGAAGCTCTGAATCATCATCATCATCATCCTCATCATCATCATCAAGGGTCTTATAATGTGACGCCTCTTAGTGTCGTTGATCCCTCGAGATTCTGTGGGCAGTTTCCGGTTACACAGCAGCCGCATTTGATGCTTTCCGGTGGAAAAGACGATTTGGGTCCATTAGCTATGCTTGAAGATAGTGTGAAGAAGCTCAAGAGTCCTAAAACTTCTCAGACGCGGAATCTAACTAAAGCTCAGATAGATTCTGCGTTAGATTCTCTCTCTGATTGGGTTTTTGAATCTTGTGGCTCTGTTTCTTTATCTGGTCTCGAGCATCCAAAGTTGAGAGCTTTTTTGACGCAAGTTGGTTTACCGATCATCTCAAGGAGAGATTTTGTCACCGGAAGATTGGATTTGAAGTATGAAGATTCGAGAGCAGAGGCTGAATCGAGAATCCACGACGCAATGTTCTTTCAGATTGCTTCTGATGGATGGAAATTCGACAGCTCCGGCGAGAATTTGGTGAATTTGATTGTGAATTTGCCTAATGGGACGAGTTTGTATAGACGAGCTGTGTTTGTGAACGGAGCTGTGCCGTCTAATTACGCAGAGGAGGTTTTGTGGGAGACGGTGAGGGGTATTTGTGGTAATTCACCTCAGAGGTGTGTTGGGATTGTTTCAGATAGGTTTATGAGTAAGGCATTGAGGAATCTTGAGAGCCAGCATCAATGGATGGTGAATCTCTCTTGTCAGTTTCAAGGTTTCAACAGTTTGATTCGAGATTTTGTAAAAGAGCTTCCTTTGTTTAAATCCGTCTCTCAAAGCTGCTCGAGGCTCGTTAATTTCGTGAACAGCACGGCACAGATCCGAAACGCGGTTTGTAAGTATCAGTTGCAAGAGCAAGGAGAGACTAGGATGCTGCATTTGCCTTTGGATAGTTCTTTGTTTGAGCCATTGTATAATCTCCTTGAGGATGTGCTTAGTTTCGCTAGAGCGATTCAGCTGGTGATGCATGATGATGTGTGTAAGGCTGTTCTAATGGAGGATCATATGGCTAGAGAGGTTGGTGAAATGGTTGGAGATGTCGGGTTTTGGAACGAGGTAGAGGCGGTTTATTTGTTGTTGAAGCTTGTTAAAGAAATGGCTCGGAGAATAGAGGAAGAGAGGCCTTTGGTAGGGCAATGTTTACCGCTATGGGATGAGCTAAGGTCCAAGATAAAAGATTGGTATGCAAAGTTCAATGTTGTTGAAGAGAGGCAAGTAGAGAAGATCGTTGAGAGAAGATTCAAGAAGAGTTATCACCCAGCTTGGGCTGCGGCGTTTATACTTGATCCGCTTTACTTGATAAAAGACAGTAGCGGAAAATATCTTCCTCCATTCAAATGCTTGTCCCCGGAGCAAGAGAAAGATGTTGATAAGTTAATAACAAGGCTTGTGTCTAGAGACGAGGCACATATCGCGATGATGGAGCTGATGAAATGGAGAACAGAAGGGCTTGATCCGGTTTACGCAAGGGCGGTTCAAATGAAGGAGAGAGATCCTGTTTCGGGGAAAATGAGGATAGCTAATCCACAAAGTAGTAGACTTGTTTGGGAAACTTATCTTAGTGAGTTCAGGTCATTGGGGAGAGTTGCAGTTAGGCTTATTTTTCTCCATGCAACTTCTTGTGGTTTCAAATGCAATTCCTCTGTTTTGAGATGGGTAAATTCGAATGGAAGATCACGCGCAGCCGTGGATCGAGCTCAAAAGTTGATCTTTATATCCGCTAACTCGAAATTCGAAAGGAGAGACTTCTCCAACGAGGAAGAGAGAGATGCGGAGCTGCTCGCTATGGCGAATGGCGAAGATGATGTGCTAAACGATGTTCTCATCGATACATCCTCAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

762

Amino Acids

86.09

Weight (kDa)

8.36

Isoelectric Point (pI)

46.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7963 PF25908 27 - 110 1.5e-46 Domain of unknown function (DUF7963)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 471
AccI GTMKAC 1 cut(s) 1981
AccII CGCG 5 cut(s) 164, 637, 1274, 1859, 2124
AclWI GGATC 7 cut(s) 491, 1258, 1452, 1724, 1892, 1931, 2142
AcsI RAATTY 7 cut(s) 322, 896, 916, 925, 937, 2104, 2174
AcyI GRCGYC 2 cut(s) 255, 479
AfiI CCNNNNNNNGG 4 cut(s) 1273, 1574, 1708, 1946
Alw21I GWGCWC 1 cut(s) 2143
Alw26I GTCTC 6 cut(s) 731, 1019, 1219, 1301, 1836, 2181
AlwI GGATC 7 cut(s) 491, 1258, 1452, 1724, 1892, 1931, 2142
AlwNI CAGNNNCTG 1 cut(s) 842
Ama87I CYCGRG 3 cut(s) 502, 728, 1228
AoxI GGCC 1 cut(s) 1565
ApeKI GCWGC 6 cut(s) 535, 1224, 1318, 1712, 2126, 2221
ApoI RAATTY 7 cut(s) 322, 896, 916, 925, 937, 2104, 2174
AseI ATTAAT 1 cut(s) 125
Asp700I GAANNNNTTC 1 cut(s) 289
AspLEI GCGC 2 cut(s) 164, 2126
AspS9I GGNCC 2 cut(s) 575, 1608
AsuC2I CCSGG 1 cut(s) 1793
AsuII TTCGAA 2 cut(s) 2108, 2178
AvaI CYCGRG 3 cut(s) 502, 728, 1228
AvaII GGWCC 2 cut(s) 575, 1608
BanII GRGCYC 1 cut(s) 2143
BarI GAAGNNNNNNTAC 2 cut(s) 1179, 1211
BbsI GAAGAC 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 2143
BbvI GCAGC 6 cut(s) 547, 1211, 1305, 1699, 2138, 2208
BccI CCATC 7 cut(s) 124, 353, 881, 885, 1126, 1858, 2091
BceAI ACGGC 7 cut(s) 45, 212, 242, 398, 979, 1272, 2114
BcnI CCSGG 1 cut(s) 1793
BcoDI GTCTC 6 cut(s) 731, 1019, 1219, 1301, 1836, 2181
BfaI CTAG 4 cut(s) 1311, 1389, 1455, 1839
BisI GCNGC 8 cut(s) 536, 539, 1225, 1319, 1713, 1716, 2127, 2222
BlsI GCNGC 8 cut(s) 537, 540, 1226, 1320, 1714, 1717, 2128, 2223
Bme1390I CCNGG 1 cut(s) 1793
Bme18I GGWCC 2 cut(s) 575, 1608
BmeT110I CYCGRG 3 cut(s) 502, 728, 1228
BmgT120I GGNCC 2 cut(s) 575, 1608
BmiI GGNNCC 1 cut(s) 576
BmrFI CCNGG 1 cut(s) 1793
BmsI GCATC 7 cut(s) 307, 537, 742, 1132, 1305, 1398, 2203
BpiI GAAGAC 1 cut(s) 375
Bpu10I CCTNAGC 1 cut(s) 1604
Bpu14I TTCGAA 2 cut(s) 2108, 2178
BpuEI CTTGAG 4 cut(s) 596, 766, 1133, 1388
BpuMI CCSGG 1 cut(s) 1793
Bsa29I ATCGAT 1 cut(s) 2271
BsaHI GRCGYC 2 cut(s) 255, 479
BsaI GGTCTC 1 cut(s) 731
BsaJI CCNNGG 3 cut(s) 414, 1791, 2130
BsaWI WCCGGW 5 cut(s) 393, 523, 554, 800, 1900
BsaXI ACNNNNNCTCC 4 cut(s) 343, 373, 1004, 1034
Bsc4I CCNNNNNNNGG 4 cut(s) 1273, 1574, 1708, 1946
Bse1I ACTGG 1 cut(s) 217
Bse3DI GCAATG 2 cut(s) 870, 1586
BseCI ATCGAT 1 cut(s) 2271
BseDI CCNNGG 3 cut(s) 414, 1791, 2130
BseGI GGATG 8 cut(s) 442, 733, 896, 1137, 1320, 1378, 1603, 2276
BseLI CCNNNNNNNGG 4 cut(s) 1273, 1574, 1708, 1946
BseMI GCAATG 2 cut(s) 870, 1586
BseMII CTCAG 5 cut(s) 279, 285, 644, 668, 1070
BseNI ACTGG 1 cut(s) 217
BseRI GAGGAG 5 cut(s) 357, 360, 363, 366, 1025
BseXI GCAGC 6 cut(s) 547, 1211, 1305, 1699, 2138, 2208
BseYI CCCAGC 1 cut(s) 1702
Bsh1236I CGCG 5 cut(s) 164, 637, 1274, 1859, 2124
BshFI GGCC 1 cut(s) 1567
BshVI ATCGAT 1 cut(s) 2271
BsiHKAI GWGCWC 1 cut(s) 2143
BsiHKCI CYCGRG 3 cut(s) 502, 728, 1228
BsiSI CCGG 7 cut(s) 394, 524, 555, 801, 909, 1793, 1901
BslFI GGGAC 2 cut(s) 964, 1774
BslI CCNNNNNNNGG 4 cut(s) 1273, 1574, 1708, 1946
BsmAI GTCTC 6 cut(s) 731, 1019, 1219, 1301, 1836, 2181
BsmBI CGTCTC 3 cut(s) 1019, 1219, 1836
BsmFI GGGAC 2 cut(s) 964, 1774
BsnI GGCC 1 cut(s) 1567
Bso31I GGTCTC 1 cut(s) 731
BsoBI CYCGRG 3 cut(s) 502, 728, 1228
Bsp119I TTCGAA 2 cut(s) 2108, 2178
Bsp1286I GDGCHC 1 cut(s) 2143
Bsp68I TCGCGA 1 cut(s) 1859
BspANI GGCC 1 cut(s) 1567
BspCNI CTCAG 5 cut(s) 278, 286, 643, 667, 1069
BspDI ATCGAT 1 cut(s) 2271
BspFNI CGCG 5 cut(s) 164, 637, 1274, 1859, 2124
BspLI GGNNCC 1 cut(s) 576
BspPI GGATC 7 cut(s) 491, 1258, 1452, 1724, 1892, 1931, 2142
BspT104I TTCGAA 2 cut(s) 2108, 2178
BspTNI GGTCTC 1 cut(s) 731
BsrDI GCAATG 2 cut(s) 870, 1586
BsrI ACTGG 1 cut(s) 217
BssECI CCNNGG 3 cut(s) 414, 1791, 2130
BssNI GRCGYC 2 cut(s) 255, 479
Bst4CI ACNGT 4 cut(s) 75, 1030, 1169, 1754
Bst6I CTCTTC 4 cut(s) 1554, 1644, 1685, 2199
BstACI GRCGYC 2 cut(s) 255, 479
BstAPI GCANNNNNTGC 1 cut(s) 547
BstBI TTCGAA 2 cut(s) 2108, 2178
BstC8I GCNNGC 2 cut(s) 1121, 2226
BstDSI CCRYGG 2 cut(s) 414, 2130
BstENI CCTNNNNNAGG 1 cut(s) 1572
BstF5I GGATG 8 cut(s) 442, 733, 896, 1137, 1320, 1378, 1603, 2276
BstFNI CGCG 5 cut(s) 164, 637, 1274, 1859, 2124
BstHHI GCGC 2 cut(s) 164, 2126
BstMAI GTCTC 6 cut(s) 731, 1019, 1219, 1301, 1836, 2181
BstMWI GCNNNNNNNGC 7 cut(s) 98, 419, 547, 1324, 1408, 1712, 1856
BstSCI CCNGG 1 cut(s) 1791
BstUI CGCG 5 cut(s) 164, 637, 1274, 1859, 2124
BstV1I GCAGC 6 cut(s) 547, 1211, 1305, 1699, 2138, 2208
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 2 cut(s) 1263, 1936
BstYI RGATCY 2 cut(s) 1263, 1936
Bsu15I ATCGAT 1 cut(s) 2271
BsuRI GGCC 1 cut(s) 1567
BsuTUI ATCGAT 1 cut(s) 2271
BtgI CCRYGG 2 cut(s) 414, 2130
BtgZI GCGATG 1 cut(s) 1874
BtsCI GGATG 8 cut(s) 442, 733, 896, 1137, 1320, 1378, 1603, 2276
BtsIMutI CAGTG 2 cut(s) 210, 2289
BtuMI TCGCGA 1 cut(s) 1859
Cac8I GCNNGC 2 cut(s) 1121, 2226
CaiI CAGNNNCTG 1 cut(s) 842
CfoI GCGC 2 cut(s) 164, 2126
Cfr13I GGNCC 2 cut(s) 575, 1608
ClaI ATCGAT 1 cut(s) 2271
CseI GACGC 5 cut(s) 263, 487, 643, 766, 869
CviAII CATG 2 cut(s) 1412, 2054
DraI TTTAAA 1 cut(s) 1207
Eam1104I CTCTTC 4 cut(s) 1554, 1644, 1685, 2199
EarI CTCTTC 4 cut(s) 1554, 1644, 1685, 2199
EciI GGCGGA 2 cut(s) 95, 400
Ecl136II GAGCTC 1 cut(s) 2141
Eco147I AGGCCT 1 cut(s) 1567
Eco24I GRGCYC 1 cut(s) 2143
Eco31I GGTCTC 1 cut(s) 731
Eco47I GGWCC 2 cut(s) 575, 1608
Eco53kI GAGCTC 1 cut(s) 2141
Eco88I CYCGRG 3 cut(s) 502, 728, 1228
EcoICRI GAGCTC 1 cut(s) 2141
EcoNI CCTNNNNNAGG 1 cut(s) 1572
EcoT22I ATGCAT 1 cut(s) 1413
EcoT38I GRGCYC 1 cut(s) 2143
Esp3I CGTCTC 3 cut(s) 1019, 1219, 1836
FaeI CATG 2 cut(s) 1415, 2057
FalI AAGNNNNNCTT 2 cut(s) 2138, 2170
FaqI GGGAC 2 cut(s) 964, 1774
FatI CATG 2 cut(s) 1411, 2053
FauNDI CATATG 1 cut(s) 1449
FblI GTMKAC 1 cut(s) 1981
Fnu4HI GCNGC 8 cut(s) 536, 539, 1225, 1319, 1713, 1716, 2127, 2222
FokI GGATG 8 cut(s) 429, 720, 903, 1144, 1327, 1385, 1610, 2263
FriOI GRGCYC 1 cut(s) 2143
Fsp4HI GCNGC 8 cut(s) 536, 539, 1225, 1319, 1713, 1716, 2127, 2222
FspBI CTAG 4 cut(s) 1311, 1389, 1455, 1839
GlaI GCGC 2 cut(s) 163, 2125
GluI GCNGC 8 cut(s) 536, 539, 1225, 1319, 1713, 1716, 2127, 2222
GsaI CCCAGC 1 cut(s) 1706
HaeIII GGCC 1 cut(s) 1567
HapII CCGG 7 cut(s) 394, 524, 555, 801, 909, 1793, 1901
HgaI GACGC 5 cut(s) 263, 487, 643, 766, 869
HhaI GCGC 2 cut(s) 164, 2126
Hin1I GRCGYC 2 cut(s) 255, 479
Hin1II CATG 2 cut(s) 1415, 2057
Hin6I GCGC 2 cut(s) 162, 2124
HinP1I GCGC 2 cut(s) 162, 2124
HindIII AAGCTT 1 cut(s) 1526
HpaII CCGG 7 cut(s) 394, 524, 555, 801, 909, 1793, 1901
Hpy166II GTNNAC 6 cut(s) 770, 982, 1249, 1586, 1906, 1982
Hpy8I GTNNAC 6 cut(s) 770, 982, 1249, 1586, 1906, 1982
Hpy99I CGWCG 5 cut(s) 10, 13, 79, 257, 863
HpyAV CCTTC 5 cut(s) 113, 288, 372, 1883, 1921
HpyCH4III ACNGT 4 cut(s) 75, 1030, 1169, 1754
HpyCH4IV ACGT 2 cut(s) 307, 400
HpyCH4V TGCA 7 cut(s) 1294, 1321, 1411, 1631, 2033, 2057, 2079
HpyF10VI GCNNNNNNNGC 7 cut(s) 98, 419, 547, 1324, 1408, 1712, 1856
HpySE526I ACGT 2 cut(s) 307, 400
Hsp92I GRCGYC 2 cut(s) 255, 479
Hsp92II CATG 2 cut(s) 1415, 2057
HspAI GCGC 2 cut(s) 162, 2124
LmnI GCTCC 5 cut(s) 911, 986, 1795, 1867, 2218
Lsp1109I GCAGC 6 cut(s) 547, 1211, 1305, 1699, 2138, 2208
LweI GCATC 7 cut(s) 307, 537, 742, 1132, 1305, 1398, 2203
MaeI CTAG 4 cut(s) 1311, 1389, 1455, 1839
MaeII ACGT 2 cut(s) 307, 400
MaeIII GTNAC 6 cut(s) 102, 113, 176, 475, 526, 796
MflI RGATCY 2 cut(s) 1263, 1936
MhlI GDGCHC 1 cut(s) 2143
MlyI GAGTC 2 cut(s) 184, 625
MmeI TCCRAC 2 cut(s) 1456, 2220
Mph1103I ATGCAT 1 cut(s) 1413
MroXI GAANNNNTTC 1 cut(s) 289
MseI TTAA 8 cut(s) 125, 150, 231, 303, 1206, 1239, 1533, 1820
MslI CAYNNNNRTG 2 cut(s) 1416, 1780
MspA1I CMGCKG 1 cut(s) 1402
MspI CCGG 7 cut(s) 394, 524, 555, 801, 909, 1793, 1901
MspR9I CCNGG 1 cut(s) 1793
MvnI CGCG 5 cut(s) 164, 637, 1274, 1859, 2124
MwoI GCNNNNNNNGC 7 cut(s) 98, 419, 547, 1324, 1408, 1712, 1856
NciI CCSGG 1 cut(s) 1793
NdeI CATATG 1 cut(s) 1449
NlaIII CATG 2 cut(s) 1415, 2057
NlaIV GGNNCC 1 cut(s) 576
NmuCI GTSAC 3 cut(s) 176, 475, 796
NruI TCGCGA 1 cut(s) 1859
NsiI ATGCAT 1 cut(s) 1413
NspV TTCGAA 2 cut(s) 2108, 2178
PaeR7I CTCGAG 3 cut(s) 502, 728, 1228
PceI AGGCCT 1 cut(s) 1567
PcsI WCGNNNNNNNCGW 2 cut(s) 71, 1242
PdmI GAANNNNTTC 1 cut(s) 289
PkrI GCNGC 8 cut(s) 537, 540, 1226, 1320, 1714, 1717, 2128, 2223
PleI GAGTC 2 cut(s) 183, 624
PpsI GAGTC 2 cut(s) 183, 624
PshBI ATTAAT 1 cut(s) 125
PsiI TTATAA 1 cut(s) 471
Psp124BI GAGCTC 1 cut(s) 2143
PspFI CCCAGC 1 cut(s) 1702
PspN4I GGNNCC 1 cut(s) 576
PspPI GGNCC 2 cut(s) 575, 1608
PspXI VCTCGAGB 1 cut(s) 1228
PsrI GAACNNNNNNTAC 4 cut(s) 177, 209, 1619, 1651
PstNI CAGNNNCTG 1 cut(s) 842
PsuI RGATCY 2 cut(s) 1263, 1936
PvuII CAGCTG 1 cut(s) 1402
RruI TCGCGA 1 cut(s) 1859
RseI CAYNNNNRTG 2 cut(s) 1416, 1780
SacI GAGCTC 1 cut(s) 2143
SaqAI TTAA 8 cut(s) 125, 150, 231, 303, 1206, 1239, 1533, 1820
SatI GCNGC 8 cut(s) 536, 539, 1225, 1319, 1713, 1716, 2127, 2222
Sau96I GGNCC 2 cut(s) 575, 1608
SchI GAGTC 2 cut(s) 184, 625
ScrFI CCNGG 1 cut(s) 1793
SduI GDGCHC 1 cut(s) 2143
SfaNI GCATC 7 cut(s) 307, 537, 742, 1132, 1305, 1398, 2203
Sfr274I CTCGAG 3 cut(s) 502, 728, 1228
SfuI TTCGAA 2 cut(s) 2108, 2178
SinI GGWCC 2 cut(s) 575, 1608
SlaI CTCGAG 3 cut(s) 502, 728, 1228
SmiMI CAYNNNNRTG 2 cut(s) 1416, 1780
SmlI CTYRAG 7 cut(s) 502, 611, 728, 781, 1112, 1228, 1367
SmoI CTYRAG 7 cut(s) 502, 611, 728, 781, 1112, 1228, 1367
SseBI AGGCCT 1 cut(s) 1567
SspMI CTAG 4 cut(s) 1311, 1389, 1455, 1839
SstI GAGCTC 1 cut(s) 2143
StuI AGGCCT 1 cut(s) 1567
StyD4I CCNGG 1 cut(s) 1791
TaaI ACNGT 4 cut(s) 75, 1030, 1169, 1754
TaiI ACGT 2 cut(s) 310, 403
TauI GCSGC 2 cut(s) 541, 1718
Tru1I TTAA 8 cut(s) 125, 150, 231, 303, 1206, 1239, 1533, 1820
Tru9I TTAA 8 cut(s) 125, 150, 231, 303, 1206, 1239, 1533, 1820
TscAI CASTG 2 cut(s) 217, 2289
TseFI GTSAC 3 cut(s) 176, 475, 796
TseI GCWGC 6 cut(s) 535, 1224, 1318, 1712, 2126, 2221
Tsp45I GTSAC 3 cut(s) 176, 475, 796
TspDTI ATGAA 3 cut(s) 837, 1889, 1940
TspGWI ACGGA 3 cut(s) 369, 999, 1201
TspRI CASTG 2 cut(s) 217, 2289
VpaK11BI GGWCC 2 cut(s) 575, 1608
VspI ATTAAT 1 cut(s) 125
XagI CCTNNNNNAGG 1 cut(s) 1572
XapI RAATTY 7 cut(s) 322, 896, 916, 925, 937, 2104, 2174
XbaI TCTAGA 1 cut(s) 1838
XhoI CTCGAG 3 cut(s) 502, 728, 1228
XmiI GTMKAC 1 cut(s) 1981
XmnI GAANNNNTTC 1 cut(s) 289
XspI CTAG 4 cut(s) 1311, 1389, 1455, 1839
Zsp2I ATGCAT 1 cut(s) 1413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.