Rmu_sc0012376.1_g000005

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0012376.1
OG0001384
Physical Location & Seq
Reverse (-)
10352 .. 10916
565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0012376.1_g000005.1.cds

Sequence Viewer

Length: 372 bp
atggccggacgactcggtgaaggggcttggtactgggcccacctaaagcccattctggttcataacaccgacaccaggctacccaaggccttgaagctccggtgcactctctgcgaggccggtttcttggcgtcgaactcgtcgcgcacggcgtcggagcacctaaagcgcaagacttgccccaatttcagctccgtttccaacccatttcgtctccgtcgccaatatcgacttctttgcccaactccgctccgcggccgctgtcctaccattgccgaaatttttgctcttttgagcctgttggtgtttggcagagatcagggtaatcttgatgaaattggaagttcagggactaacatgaagattgagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659

Protein Analysis

123

Amino Acids

13.76

Weight (kDa)

9.89

Isoelectric Point (pI)

35.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 250
AccII CGCG 2 cut(s) 145, 255
AciI CCGC 4 cut(s) 248, 253, 255, 259
AcoI YGGCCR 2 cut(s) 3, 256
AcsI RAATTY 1 cut(s) 279
AcyI GRCGYC 2 cut(s) 131, 152
AfaI GTAC 1 cut(s) 32
AfiI CCNNNNNNNGG 2 cut(s) 75, 254
AgsI TTSAA 1 cut(s) 94
AjnI CCWGG 1 cut(s) 74
AjuI GAANNNNNNNTTGG 2 cut(s) 216, 248
AluBI AGCT 2 cut(s) 97, 192
AluI AGCT 2 cut(s) 97, 192
Alw21I GWGCWC 2 cut(s) 107, 162
Alw26I GTCTC 1 cut(s) 218
Alw44I GTGCAC 1 cut(s) 103
AoxI GGCC 5 cut(s) 3, 36, 87, 117, 256
ApaI GGGCCC 1 cut(s) 40
ApaLI GTGCAC 1 cut(s) 103
ApoI RAATTY 1 cut(s) 279
AspLEI GCGC 2 cut(s) 147, 171
AspS9I GGNCC 2 cut(s) 36, 37
AsuHPI GGTGA 1 cut(s) 29
BaeGI GKGCMC 2 cut(s) 40, 107
BanII GRGCYC 1 cut(s) 40
Bbv12I GWGCWC 2 cut(s) 107, 162
BceAI ACGGC 1 cut(s) 165
BcgI CGANNNNNNTGC 4 cut(s) 219, 253, 266, 300
BciT130I CCWGG 1 cut(s) 76
BcoDI GTCTC 1 cut(s) 218
BisI GCNGC 2 cut(s) 256, 259
BlsI GCNGC 2 cut(s) 257, 260
Bme1390I CCNGG 1 cut(s) 76
BmgT120I GGNCC 2 cut(s) 36, 37
BmiI GGNNCC 1 cut(s) 38
BmrFI CCNGG 1 cut(s) 76
BmrI ACTGGG 1 cut(s) 43
BmuI ACTGGG 1 cut(s) 43
BsaHI GRCGYC 2 cut(s) 131, 152
BsaJI CCNNGG 2 cut(s) 84, 253
BsaWI WCCGGW 1 cut(s) 99
Bsc4I CCNNNNNNNGG 2 cut(s) 75, 254
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 1 cut(s) 38
Bse3DI GCAATG 1 cut(s) 270
BseBI CCWGG 1 cut(s) 76
BseDI CCNNGG 2 cut(s) 84, 253
BseLI CCNNNNNNNGG 2 cut(s) 75, 254
BseMI GCAATG 1 cut(s) 270
BseNI ACTGG 1 cut(s) 38
BseSI GKGCMC 2 cut(s) 40, 107
BseX3I CGGCCG 1 cut(s) 256
Bsh1236I CGCG 2 cut(s) 145, 255
Bsh1285I CGRYCG 1 cut(s) 259
BshFI GGCC 5 cut(s) 5, 38, 89, 119, 258
BsiEI CGRYCG 1 cut(s) 259
BsiHKAI GWGCWC 2 cut(s) 107, 162
BsiSI CCGG 3 cut(s) 6, 100, 120
BslFI GGGAC 1 cut(s) 364
BslI CCNNNNNNNGG 2 cut(s) 75, 254
BsmAI GTCTC 1 cut(s) 218
BsmBI CGTCTC 1 cut(s) 218
BsmFI GGGAC 1 cut(s) 364
BsnI GGCC 5 cut(s) 5, 38, 89, 119, 258
Bsp120I GGGCCC 1 cut(s) 36
Bsp1286I GDGCHC 3 cut(s) 40, 107, 162
Bsp143I GATC 1 cut(s) 316
BspACI CCGC 4 cut(s) 248, 253, 255, 259
BspANI GGCC 5 cut(s) 5, 38, 89, 119, 258
BspFNI CGCG 2 cut(s) 145, 255
BspLI GGNNCC 1 cut(s) 38
BsrBI CCGCTC 1 cut(s) 250
BsrDI GCAATG 1 cut(s) 270
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 1 cut(s) 38
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 2 cut(s) 84, 253
BssMI GATC 1 cut(s) 316
BssNI GRCGYC 2 cut(s) 131, 152
BssT1I CCWWGG 1 cut(s) 84
Bst2UI CCWGG 1 cut(s) 76
BstACI GRCGYC 2 cut(s) 131, 152
BstAPI GCANNNNNTGC 2 cut(s) 111, 177
BstDSI CCRYGG 1 cut(s) 253
BstFNI CGCG 2 cut(s) 145, 255
BstHHI GCGC 2 cut(s) 147, 171
BstKTI GATC 1 cut(s) 319
BstMAI GTCTC 1 cut(s) 218
BstMBI GATC 1 cut(s) 316
BstMCI CGRYCG 1 cut(s) 259
BstMWI GCNNNNNNNGC 3 cut(s) 111, 166, 177
BstNI CCWGG 1 cut(s) 76
BstSCI CCNGG 1 cut(s) 74
BstSLI GKGCMC 2 cut(s) 40, 107
BstUI CGCG 2 cut(s) 145, 255
BstZI CGGCCG 1 cut(s) 256
BsuRI GGCC 5 cut(s) 5, 38, 89, 119, 258
BtgI CCRYGG 1 cut(s) 253
CciNI GCGGCCGC 1 cut(s) 256
CfoI GCGC 2 cut(s) 147, 171
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 36, 37
Cfr42I CCGCGG 1 cut(s) 256
CseI GACGC 2 cut(s) 120, 141
Csp6I GTAC 1 cut(s) 31
CviAII CATG 1 cut(s) 358
CviQI GTAC 1 cut(s) 31
DpnI GATC 1 cut(s) 318
DpnII GATC 1 cut(s) 316
EaeI YGGCCR 2 cut(s) 3, 256
EagI CGGCCG 1 cut(s) 256
EclXI CGGCCG 1 cut(s) 256
Eco130I CCWWGG 1 cut(s) 84
Eco147I AGGCCT 1 cut(s) 89
Eco24I GRGCYC 1 cut(s) 40
Eco52I CGGCCG 1 cut(s) 256
EcoRII CCWGG 1 cut(s) 74
EcoT14I CCWWGG 1 cut(s) 84
EcoT38I GRGCYC 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 84
Esp3I CGTCTC 1 cut(s) 218
FaeI CATG 1 cut(s) 361
FaiI YATR 2 cut(s) 63, 359
FaqI GGGAC 1 cut(s) 364
FatI CATG 1 cut(s) 357
Fnu4HI GCNGC 2 cut(s) 256, 259
FriOI GRGCYC 1 cut(s) 40
Fsp4HI GCNGC 2 cut(s) 256, 259
GlaI GCGC 2 cut(s) 146, 170
GluI GCNGC 2 cut(s) 256, 259
HaeIII GGCC 5 cut(s) 5, 38, 89, 119, 258
HapII CCGG 3 cut(s) 6, 100, 120
HgaI GACGC 2 cut(s) 120, 141
HhaI GCGC 2 cut(s) 147, 171
Hin1I GRCGYC 2 cut(s) 131, 152
Hin1II CATG 1 cut(s) 361
Hin6I GCGC 2 cut(s) 145, 169
HinP1I GCGC 2 cut(s) 145, 169
HinfI GANTC 1 cut(s) 12
HpaII CCGG 3 cut(s) 6, 100, 120
HphI GGTGA 1 cut(s) 29
Hpy166II GTNNAC 1 cut(s) 105
Hpy188I TCNGA 1 cut(s) 157
Hpy188III TCNNGA 1 cut(s) 329
Hpy8I GTNNAC 1 cut(s) 105
Hpy99I CGWCG 4 cut(s) 136, 145, 157, 222
HpyAV CCTTC 1 cut(s) 14
HpyCH4V TGCA 1 cut(s) 105
HpyF10VI GCNNNNNNNGC 3 cut(s) 111, 166, 177
Hsp92I GRCGYC 2 cut(s) 131, 152
Hsp92II CATG 1 cut(s) 361
HspAI GCGC 2 cut(s) 145, 169
KspI CCGCGG 1 cut(s) 256
Kzo9I GATC 1 cut(s) 316
LmnI GCTCC 4 cut(s) 102, 157, 197, 255
MalI GATC 1 cut(s) 318
MbiI CCGCTC 1 cut(s) 250
MboI GATC 1 cut(s) 316
MhlI GDGCHC 3 cut(s) 40, 107, 162
MluCI AATT 3 cut(s) 184, 279, 336
MlyI GAGTC 1 cut(s) 6
MmeI TCCRAC 2 cut(s) 135, 225
MnlI CCTC 1 cut(s) 109
MspA1I CMGCKG 2 cut(s) 255, 261
MspI CCGG 3 cut(s) 6, 100, 120
MspR9I CCNGG 1 cut(s) 76
MvaI CCWGG 1 cut(s) 76
MvnI CGCG 2 cut(s) 145, 255
MwoI GCNNNNNNNGC 3 cut(s) 111, 166, 177
NdeII GATC 1 cut(s) 316
NlaIII CATG 1 cut(s) 361
NlaIV GGNNCC 1 cut(s) 38
NotI GCGGCCGC 1 cut(s) 256
PceI AGGCCT 1 cut(s) 89
PcsI WCGNNNNNNNCGW 2 cut(s) 149, 226
PkrI GCNGC 2 cut(s) 257, 260
PleI GAGTC 1 cut(s) 6
PpsI GAGTC 1 cut(s) 6
Psp6I CCWGG 1 cut(s) 74
PspGI CCWGG 1 cut(s) 74
PspN4I GGNNCC 1 cut(s) 38
PspOMI GGGCCC 1 cut(s) 36
PspPI GGNCC 2 cut(s) 36, 37
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
SacII CCGCGG 1 cut(s) 256
SatI GCNGC 2 cut(s) 256, 259
Sau3AI GATC 1 cut(s) 316
Sau96I GGNCC 2 cut(s) 36, 37
SchI GAGTC 1 cut(s) 6
ScrFI CCNGG 1 cut(s) 76
SduI GDGCHC 3 cut(s) 40, 107, 162
SetI ASST 4 cut(s) 45, 99, 165, 194
Sfr303I CCGCGG 1 cut(s) 256
SgrBI CCGCGG 1 cut(s) 256
Sse9I AATT 3 cut(s) 184, 279, 336
SseBI AGGCCT 1 cut(s) 89
SsiI CCGC 4 cut(s) 248, 253, 255, 259
StuI AGGCCT 1 cut(s) 89
StyD4I CCNGG 1 cut(s) 74
StyI CCWWGG 1 cut(s) 84
TaqI TCGA 2 cut(s) 134, 229
TasI AATT 3 cut(s) 184, 279, 336
TauI GCSGC 2 cut(s) 258, 261
TspDTI ATGAA 2 cut(s) 50, 348
TspGWI ACGGA 2 cut(s) 184, 206
VneI GTGCAC 1 cut(s) 103
XapI RAATTY 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.