MD07G1257500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001384
Physical Location & Seq
Reverse (-)
32375061 .. 32380145
5085 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1257500.v1.1.491

Sequence Viewer

Length: 2007 bp
ATGGCGTCCACAAACTCAACCCCAACAGAACCATCTCTCTCGCCGGACGACATAGCCGCCAGGGCCCTCAACCGGCGATTCGAAAGCCTGGTAACTGTTCGAACAAAGGCCATCAAGGGGAAAGGAGCTTGGTACTGGGCTCACTTGGAGCCCGTTCTCGTTCGCATCCCCAACTCCAACATCCCCAAGGCCGTCAAGCTCAAGTGCTCACTCTGCGACGCCGTCTTCTCCGCCTCCAACCCCTCCAGAACCGCCTCAGAGCATCTCAAACGGGGTACCTGCCCGAATTTCACCTCTGTTTTGCGGCCCAATTCATCGGCGGCGGCATCTCCGGTTCCGATTTCTTCCTTGCCTTCTCCGTCGTCTCACAATCACAGAAAGCGGAGCTCTCAAATGGGTACTAGTCCTTCTCCTATTTCTCAAGCTCCTCCGCACATTAGTCCTAGTCCGATACAGGTTCACTCTTTAGCTATGATCGAGTCTTCCCGCTTCGGCGGTGACCATAATTTCTCACAGTCTCCAAACCCTGTTGGGATTGCGAGAAGCATGGGGCTGGATCAGCACCATTTGGTGTTATCTGGTGGGAAGGAGGATTTGGGTGCTTTGGCAATGTTAGAAAATAGTGTGAAGAAACTTAAGAGTCCCAAAGCTTCACCTGGTGCCACTCTTAGTAAGGAGCAGATTGATTCTGCACTTCAACTACTGTCTGAGTGGTTCTATGAGTCATGTGGGTCAGTCTCATTTTCGAGCCTCGAGCATCCCAAGTTCAGGGCTTTCCTTAGCCAGGTGGGCTTGCCTGCATTGCTGCAGCGTGAGCTTTCTGGTGCTCGACTTGATGCTAAGTTTGATGAGGTGAAAGCTGAGTCTGAAGCTAGGATTAGAGATGCAATGTTTTTCCAAGTTGCTTCTGATGGGTGGAAGAGCGGGGATTCTTGTGGGGAAGAGAATGTTGTTAAGTTCACAGTTAATCTTCCAAATGGGATTAGTGTTTTCCAGAAGGTGGTGTTTACTGGCGGGTCAGTTTCTTCGAAATATGCGGAGGAGGTGTTGTGGGATTCAGTTACTGGAATATGTGGGAATGCTGTGCAGCGTTGTGTGGGGATCGTTGCAGACAAATATAAGGCCAAGGCATTGAGGAACTTGGAGACTCAGAACCATTGGATGGTGAATGTCTCCTGGATGATTCAAACTGAAGGATTTTGGAATGAATTGGAGGCAGTTTATTCACTTGTTAAGATGATCAGAGGGATGACTCAGGAGATTGAGGCTGAGAGGCCATTAATCGGGCAATGTCTACCCCTTTGGGAAGAATTGAGAACAAAAGTAAAGGATTGGTGTGCCAAGTTCAACATTGTTGAAGGACCTGTTGAGGAAATAGTTGAAAAACGATTCAGAAAGAACTACCACCCAGCATGGTCGGCTGCATTTATCCTTGACCCGATATACTTGATGAGGGACACTAGTGGAAAGTACCTTCCACCATTCAAGTGCTTGACACATGAGCAGGAGAAGGATGTAGATAAGCTCATTACCAGGTTGGTTTCCAGAGAAGAAGCTCATGTTGCATTGATGGAGCTCATGAAATGGAGAACGGAAGGAATGGACCCACTTTACGCTCAGGCAGTGCAGGTTAAACAGCGAGATCCTGTAACTGGAAAGATGAAAATGGCTAACCCACAGAGCAGCAGACTTGTGTGGGAAACTTGTCTAAGTGAGTTAAAGACATTGGGGAGGGTTGCAGTGAGACTTATCTTCCTCCATGCAACCTCGTGTGGATTTAAGTGCAATTGGTCTTTCATGAGATGGATTCGTGTACATAGGCACTCAAGGGTAGGCCTGGAAAAGGCACAGAAGATGATATTTATTGCAGCTCATGCCAAAATTGAGAGAAGGGATCTTTCTAGTGAGGAAGAAAAGGAAGCAGAGCTGTTTGGGACTGCAGACGTTGAGGATGACATGCTGACTGAGGTCTTTTCCGATGCACCCACAGTGATAGGGTTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

669

Amino Acids

74.38

Weight (kDa)

8.63

Isoelectric Point (pI)

52.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7963 PF25908 16 - 98 1.5e-44 Domain of unknown function (DUF7963)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 287, 1618
Acc65I GGTACC 1 cut(s) 275
AccB1I GGYRCC 2 cut(s) 275, 659
AccB7I CCANNNNNTGG 2 cut(s) 1000, 1162
AccBSI CCGCTC 1 cut(s) 924
AccI GTMKAC 1 cut(s) 1294
AclWI GGATC 4 cut(s) 564, 1109, 1637, 1902
AcsI RAATTY 1 cut(s) 286
AcuI CTGAAG 2 cut(s) 888, 1212
AcyI GRCGYC 2 cut(s) 5, 219
AdeI CACNNNGTG 1 cut(s) 659
AfaI GTAC 5 cut(s) 134, 277, 400, 1472, 1815
AfiI CCNNNNNNNGG 9 cut(s) 72, 117, 768, 784, 1000, 1162, 1283, 1652, 1843
AflII CTTAAG 1 cut(s) 635
AgsI TTSAA 6 cut(s) 698, 1187, 1348, 1358, 1382, 1486
AhlI ACTAGT 2 cut(s) 401, 1460
AjnI CCWGG 7 cut(s) 59, 87, 655, 783, 1175, 1532, 1836
AjuI GAANNNNNNNTTGG 2 cut(s) 578, 610
Alw21I GWGCWC 4 cut(s) 209, 389, 829, 1578
Alw26I GTCTC 6 cut(s) 369, 522, 742, 1139, 1177, 1738
AlwI GGATC 4 cut(s) 564, 1109, 1637, 1902
AlwNI CAGNNNCTG 1 cut(s) 1064
Ama87I CYCGRG 1 cut(s) 752
AoxI GGCC 7 cut(s) 63, 108, 189, 305, 1122, 1274, 1834
ApaI GGGCCC 1 cut(s) 67
ApeKI GCWGC 6 cut(s) 805, 808, 1087, 1421, 1683, 1868
ApoI RAATTY 1 cut(s) 286
AseI ATTAAT 1 cut(s) 1280
Asp718I GGTACC 1 cut(s) 275
AspS9I GGNCC 5 cut(s) 63, 64, 306, 1361, 1603
AsuHPI GGTGA 5 cut(s) 283, 509, 645, 865, 1177
AsuII TTCGAA 3 cut(s) 81, 100, 1028
AvaI CYCGRG 1 cut(s) 752
AvaII GGWCC 2 cut(s) 1361, 1603
BaeGI GKGCMC 1 cut(s) 67
BanI GGYRCC 2 cut(s) 275, 659
BanII GRGCYC 5 cut(s) 67, 142, 153, 389, 1578
BarI GAAGNNNNNNTAC 2 cut(s) 391, 423
BauI CACGAG 1 cut(s) 1768
BbsI GAAGAC 2 cut(s) 217, 474
Bbv12I GWGCWC 4 cut(s) 209, 389, 829, 1578
BbvI GCAGC 6 cut(s) 792, 820, 1099, 1408, 1695, 1880
BccI CCATC 6 cut(s) 40, 119, 905, 1156, 1564, 1797
BceAI ACGGC 2 cut(s) 176, 206
BciT130I CCWGG 7 cut(s) 61, 89, 657, 785, 1177, 1534, 1838
BclI TGATCA 1 cut(s) 1239
BcoDI GTCTC 6 cut(s) 369, 522, 742, 1139, 1177, 1738
BcuI ACTAGT 2 cut(s) 401, 1460
BfaI CTAG 5 cut(s) 402, 444, 873, 1461, 1902
BfmI CTRYAG 2 cut(s) 806, 1938
BfrI CTTAAG 1 cut(s) 635
BfuAI ACCTGC 2 cut(s) 287, 1618
BglI GCCNNNNNGGC 2 cut(s) 62, 789
Bme1390I CCNGG 7 cut(s) 61, 89, 657, 785, 1177, 1534, 1838
Bme18I GGWCC 2 cut(s) 1361, 1603
BmeT110I CYCGRG 1 cut(s) 752
BmgT120I GGNCC 5 cut(s) 63, 64, 306, 1361, 1603
BmiI GGNNCC 6 cut(s) 65, 150, 277, 336, 661, 1605
BmrFI CCNGG 7 cut(s) 61, 89, 657, 785, 1177, 1534, 1838
BmrI ACTGGG 1 cut(s) 145
BmsI GCATC 7 cut(s) 174, 271, 335, 766, 826, 874, 1969
BmuI ACTGGG 1 cut(s) 145
BoxI GACNNNNGTC 1 cut(s) 1967
BpiI GAAGAC 2 cut(s) 217, 474
BpmI CTGGAG 1 cut(s) 229
Bpu10I CCTNAGC 2 cut(s) 779, 1617
Bpu14I TTCGAA 3 cut(s) 81, 100, 1028
BpuEI CTTGAG 3 cut(s) 185, 405, 1810
BsaHI GRCGYC 2 cut(s) 5, 219
BsaJI CCNNGG 3 cut(s) 60, 186, 1125
BsaWI WCCGGW 1 cut(s) 331
BsaXI ACNNNNNCTCC 4 cut(s) 1031, 1034, 1061, 1064
Bsc4I CCNNNNNNNGG 9 cut(s) 72, 117, 768, 784, 1000, 1162, 1283, 1652, 1843
Bse118I RCCGGY 1 cut(s) 72
Bse1I ACTGG 4 cut(s) 140, 1015, 1069, 1657
Bse3DI GCAATG 4 cut(s) 615, 800, 894, 1295
BseBI CCWGG 7 cut(s) 61, 89, 657, 785, 1177, 1534, 1838
BseDI CCNNGG 3 cut(s) 60, 186, 1125
BseGI GGATG 8 cut(s) 165, 180, 757, 1167, 1185, 1254, 1519, 1957
BseLI CCNNNNNNNGG 9 cut(s) 72, 117, 768, 784, 1000, 1162, 1283, 1652, 1843
BseMI GCAATG 4 cut(s) 615, 800, 894, 1295
BseMII CTCAG 8 cut(s) 270, 699, 852, 1163, 1260, 1268, 1631, 1956
BseNI ACTGG 4 cut(s) 140, 1015, 1069, 1657
BseRI GAGGAG 2 cut(s) 417, 1055
BseSI GKGCMC 1 cut(s) 67
BseXI GCAGC 6 cut(s) 792, 820, 1099, 1408, 1695, 1880
BseYI CCCAGC 1 cut(s) 1408
BsgI GTGCAG 3 cut(s) 675, 1106, 1646
BshFI GGCC 7 cut(s) 65, 110, 191, 307, 1124, 1276, 1836
BshNI GGYRCC 2 cut(s) 275, 659
BsiHKAI GWGCWC 4 cut(s) 209, 389, 829, 1578
BsiHKCI CYCGRG 1 cut(s) 752
BsiSI CCGG 3 cut(s) 44, 73, 332
BslFI GGGAC 3 cut(s) 627, 1469, 1948
BslI CCNNNNNNNGG 9 cut(s) 72, 117, 768, 784, 1000, 1162, 1283, 1652, 1843
BsmAI GTCTC 6 cut(s) 369, 522, 742, 1139, 1177, 1738
BsmBI CGTCTC 1 cut(s) 369
BsmFI GGGAC 3 cut(s) 627, 1469, 1948
BsmI GAATGC 1 cut(s) 1084
BsnI GGCC 7 cut(s) 65, 110, 191, 307, 1124, 1276, 1836
BsoBI CYCGRG 1 cut(s) 752
Bsp119I TTCGAA 3 cut(s) 81, 100, 1028
Bsp120I GGGCCC 1 cut(s) 63
Bsp1286I GDGCHC 7 cut(s) 67, 142, 153, 209, 389, 829, 1578
Bsp1407I TGTACA 1 cut(s) 1813
Bsp143I GATC 6 cut(s) 474, 556, 1101, 1239, 1642, 1894
BspANI GGCC 7 cut(s) 65, 110, 191, 307, 1124, 1276, 1836
BspCNI CTCAG 8 cut(s) 269, 700, 853, 1162, 1261, 1267, 1630, 1957
BspHI TCATGA 2 cut(s) 1578, 1797
BspLI GGNNCC 6 cut(s) 65, 150, 277, 336, 661, 1605
BspMAI CTGCAG 2 cut(s) 810, 1942
BspMI ACCTGC 2 cut(s) 287, 1618
BspPI GGATC 4 cut(s) 564, 1109, 1637, 1902
BspQI GCTCTTC 1 cut(s) 914
BspT104I TTCGAA 3 cut(s) 81, 100, 1028
BspT107I GGYRCC 2 cut(s) 275, 659
BspTI CTTAAG 1 cut(s) 635
BsrBI CCGCTC 1 cut(s) 924
BsrDI GCAATG 4 cut(s) 615, 800, 894, 1295
BsrFI RCCGGY 1 cut(s) 72
BsrGI TGTACA 1 cut(s) 1813
BsrI ACTGG 4 cut(s) 140, 1015, 1069, 1657
BssAI RCCGGY 1 cut(s) 72
BssECI CCNNGG 3 cut(s) 60, 186, 1125
BssMI GATC 6 cut(s) 474, 556, 1101, 1239, 1642, 1894
BssNI GRCGYC 2 cut(s) 5, 219
BssSI CACGAG 1 cut(s) 1768
BssT1I CCWWGG 2 cut(s) 186, 1125
Bst2BI CACGAG 1 cut(s) 1768
Bst2UI CCWGG 7 cut(s) 61, 89, 657, 785, 1177, 1534, 1838
Bst4CI ACNGT 5 cut(s) 97, 516, 705, 964, 1990
Bst6I CTCTTC 2 cut(s) 914, 936
BstACI GRCGYC 2 cut(s) 5, 219
BstAFI CTTAAG 1 cut(s) 635
BstAPI GCANNNNNTGC 1 cut(s) 1874
BstAUI TGTACA 1 cut(s) 1813
BstBI TTCGAA 3 cut(s) 81, 100, 1028
BstC8I GCNNGC 2 cut(s) 794, 798
BstEII GGTNACC 1 cut(s) 497
BstENI CCTNNNNNAGG 2 cut(s) 782, 1841
BstF5I GGATG 8 cut(s) 165, 180, 757, 1167, 1185, 1254, 1519, 1957
BstKTI GATC 6 cut(s) 477, 559, 1104, 1242, 1645, 1897
BstMAI GTCTC 6 cut(s) 369, 522, 742, 1139, 1177, 1738
BstMBI GATC 6 cut(s) 474, 556, 1101, 1239, 1642, 1894
BstMWI GCNNNNNNNGC 9 cut(s) 62, 213, 559, 789, 802, 814, 1418, 1562, 1874
BstNI CCWGG 7 cut(s) 61, 89, 657, 785, 1177, 1534, 1838
BstNSI RCATGY 1 cut(s) 1960
BstPAI GACNNNNGTC 1 cut(s) 1967
BstPI GGTNACC 1 cut(s) 497
BstSCI CCNGG 7 cut(s) 59, 87, 655, 783, 1175, 1532, 1836
BstSFI CTRYAG 2 cut(s) 806, 1938
BstSLI GKGCMC 1 cut(s) 67
BstV1I GCAGC 6 cut(s) 792, 820, 1099, 1408, 1695, 1880
BstV2I GAAGAC 2 cut(s) 217, 474
BstX2I RGATCY 2 cut(s) 1642, 1894
BstYI RGATCY 2 cut(s) 1642, 1894
BsuRI GGCC 7 cut(s) 65, 110, 191, 307, 1124, 1276, 1836
BtsCI GGATG 8 cut(s) 165, 180, 757, 1167, 1185, 1254, 1519, 1957
BtsI GCAGTG 2 cut(s) 1629, 1746
BtsIMutI CAGTG 3 cut(s) 1629, 1746, 1995
BveI ACCTGC 2 cut(s) 287, 1618
Cac8I GCNNGC 2 cut(s) 794, 798
CaiI CAGNNNCTG 1 cut(s) 1064
CciI TCATGA 2 cut(s) 1578, 1797
Cfr10I RCCGGY 1 cut(s) 72
Cfr13I GGNCC 5 cut(s) 63, 64, 306, 1361, 1603
CseI GACGC 1 cut(s) 227
CsiI ACCWGGT 2 cut(s) 655, 1532
Csp6I GTAC 5 cut(s) 133, 276, 399, 1471, 1814
CviQI GTAC 5 cut(s) 133, 276, 399, 1471, 1814
DpnI GATC 6 cut(s) 476, 558, 1103, 1241, 1644, 1896
DpnII GATC 6 cut(s) 474, 556, 1101, 1239, 1642, 1894
DraIII CACNNNGTG 1 cut(s) 659
Eam1104I CTCTTC 2 cut(s) 914, 936
EarI CTCTTC 2 cut(s) 914, 936
EciI GGCGGA 1 cut(s) 220
Ecl136II GAGCTC 2 cut(s) 387, 1576
Eco130I CCWWGG 2 cut(s) 186, 1125
Eco147I AGGCCT 1 cut(s) 1836
Eco24I GRGCYC 5 cut(s) 67, 142, 153, 389, 1578
Eco47I GGWCC 2 cut(s) 1361, 1603
Eco53kI GAGCTC 2 cut(s) 387, 1576
Eco57I CTGAAG 2 cut(s) 888, 1212
Eco88I CYCGRG 1 cut(s) 752
Eco91I GGTNACC 1 cut(s) 497
EcoICRI GAGCTC 2 cut(s) 387, 1576
EcoNI CCTNNNNNAGG 2 cut(s) 782, 1841
EcoO109I RGGNCCY 3 cut(s) 63, 64, 1361
EcoO65I GGTNACC 1 cut(s) 497
EcoRII CCWGG 7 cut(s) 59, 87, 655, 783, 1175, 1532, 1836
EcoT14I CCWWGG 2 cut(s) 186, 1125
EcoT38I GRGCYC 5 cut(s) 67, 142, 153, 389, 1578
ErhI CCWWGG 2 cut(s) 186, 1125
Esp3I CGTCTC 1 cut(s) 369
FaqI GGGAC 3 cut(s) 627, 1469, 1948
FauI CCCGC 3 cut(s) 494, 917, 1007
FbaI TGATCA 1 cut(s) 1239
FblI GTMKAC 1 cut(s) 1294
FokI GGATG 8 cut(s) 152, 167, 744, 1174, 1192, 1261, 1526, 1964
FriOI GRGCYC 5 cut(s) 67, 142, 153, 389, 1578
FspBI CTAG 5 cut(s) 402, 444, 873, 1461, 1902
GsaI CCCAGC 1 cut(s) 1412
GsuI CTGGAG 1 cut(s) 229
HaeIII GGCC 7 cut(s) 65, 110, 191, 307, 1124, 1276, 1836
HapII CCGG 3 cut(s) 44, 73, 332
HgaI GACGC 1 cut(s) 227
Hin1I GRCGYC 2 cut(s) 5, 219
HindIII AAGCTT 1 cut(s) 648
HpaII CCGG 3 cut(s) 44, 73, 332
HphI GGTGA 5 cut(s) 283, 509, 645, 865, 1177
Hpy166II GTNNAC 6 cut(s) 9, 460, 960, 1008, 1295, 1814
Hpy188III TCNNGA 7 cut(s) 246, 994, 1256, 1545, 1579, 1798, 2004
Hpy8I GTNNAC 6 cut(s) 9, 460, 960, 1008, 1295, 1814
Hpy99I CGWCG 2 cut(s) 221, 364
HpyCH4III ACNGT 5 cut(s) 97, 516, 705, 964, 1990
HpyCH4IV ACGT 1 cut(s) 1944
HpyF10VI GCNNNNNNNGC 9 cut(s) 62, 213, 559, 789, 802, 814, 1418, 1562, 1874
HpySE526I ACGT 1 cut(s) 1944
Hsp92I GRCGYC 2 cut(s) 5, 219
KpnI GGTACC 1 cut(s) 279
Ksp22I TGATCA 1 cut(s) 1239
Kzo9I GATC 6 cut(s) 474, 556, 1101, 1239, 1642, 1894
LguI GCTCTTC 1 cut(s) 914
LmnI GCTCC 6 cut(s) 125, 148, 384, 430, 676, 1573
Lsp1109I GCAGC 6 cut(s) 792, 820, 1099, 1408, 1695, 1880
LweI GCATC 7 cut(s) 174, 271, 335, 766, 826, 874, 1969
MabI ACCWGGT 2 cut(s) 655, 1532
MaeI CTAG 5 cut(s) 402, 444, 873, 1461, 1902
MaeII ACGT 1 cut(s) 1944
MaeIII GTNAC 4 cut(s) 91, 497, 1060, 1648
MalI GATC 6 cut(s) 476, 558, 1103, 1241, 1644, 1896
MbiI CCGCTC 1 cut(s) 924
MboI GATC 6 cut(s) 474, 556, 1101, 1239, 1642, 1894
MfeI CAATTG 1 cut(s) 1786
MflI RGATCY 2 cut(s) 1642, 1894
MhlI GDGCHC 7 cut(s) 67, 142, 153, 209, 389, 829, 1578
MluCI AATT 7 cut(s) 286, 310, 505, 1208, 1310, 1786, 1881
MlyI GAGTC 6 cut(s) 488, 649, 731, 872, 1141, 1246
MmeI TCCRAC 2 cut(s) 201, 261
MseI TTAA 8 cut(s) 636, 954, 966, 1233, 1280, 1632, 1718, 1779
MslI CAYNNNNRTG 1 cut(s) 1486
MspCI CTTAAG 1 cut(s) 635
MspI CCGG 3 cut(s) 44, 73, 332
MspR9I CCNGG 7 cut(s) 61, 89, 657, 785, 1177, 1534, 1838
MunI CAATTG 1 cut(s) 1786
Mva1269I GAATGC 1 cut(s) 1084
MvaI CCWGG 7 cut(s) 61, 89, 657, 785, 1177, 1534, 1838
MwoI GCNNNNNNNGC 9 cut(s) 62, 213, 559, 789, 802, 814, 1418, 1562, 1874
NdeII GATC 6 cut(s) 474, 556, 1101, 1239, 1642, 1894
NlaIV GGNNCC 6 cut(s) 65, 150, 277, 336, 661, 1605
NmuCI GTSAC 1 cut(s) 497
NspI RCATGY 1 cut(s) 1960
NspV TTCGAA 3 cut(s) 81, 100, 1028
PaeR7I CTCGAG 1 cut(s) 752
PagI TCATGA 2 cut(s) 1578, 1797
PceI AGGCCT 1 cut(s) 1836
PciSI GCTCTTC 1 cut(s) 914
PctI GAATGC 1 cut(s) 1084
PfeI GAWTC 7 cut(s) 78, 686, 929, 1055, 1183, 1389, 1807
PflFI GACNNNGTC 1 cut(s) 221
PflMI CCANNNNNTGG 2 cut(s) 1000, 1162
PfoI TCCNGGA 1 cut(s) 1175
PleI GAGTC 6 cut(s) 487, 648, 730, 871, 1141, 1246
PpsI GAGTC 6 cut(s) 487, 648, 730, 871, 1141, 1246
PpuMI RGGWCCY 1 cut(s) 1361
PshAI GACNNNNGTC 1 cut(s) 1967
PshBI ATTAAT 1 cut(s) 1280
Psp124BI GAGCTC 2 cut(s) 389, 1578
Psp5II RGGWCCY 1 cut(s) 1361
Psp6I CCWGG 7 cut(s) 59, 87, 655, 783, 1175, 1532, 1836
PspEI GGTNACC 1 cut(s) 497
PspFI CCCAGC 1 cut(s) 1408
PspGI CCWGG 7 cut(s) 59, 87, 655, 783, 1175, 1532, 1836
PspN4I GGNNCC 6 cut(s) 65, 150, 277, 336, 661, 1605
PspOMI GGGCCC 1 cut(s) 63
PspPI GGNCC 5 cut(s) 63, 64, 306, 1361, 1603
PspPPI RGGWCCY 1 cut(s) 1361
PspXI VCTCGAGB 1 cut(s) 752
PstI CTGCAG 2 cut(s) 810, 1942
PstNI CAGNNNCTG 1 cut(s) 1064
PsuI RGATCY 2 cut(s) 1642, 1894
PsyI GACNNNGTC 1 cut(s) 221
RsaI GTAC 5 cut(s) 134, 277, 400, 1472, 1815
RsaNI GTAC 5 cut(s) 133, 276, 399, 1471, 1814
RseI CAYNNNNRTG 1 cut(s) 1486
SacI GAGCTC 2 cut(s) 389, 1578
SapI GCTCTTC 1 cut(s) 914
SaqAI TTAA 8 cut(s) 636, 954, 966, 1233, 1280, 1632, 1718, 1779
Sau3AI GATC 6 cut(s) 474, 556, 1101, 1239, 1642, 1894
Sau96I GGNCC 5 cut(s) 63, 64, 306, 1361, 1603
SchI GAGTC 6 cut(s) 488, 649, 731, 872, 1141, 1246
ScrFI CCNGG 7 cut(s) 61, 89, 657, 785, 1177, 1534, 1838
SduI GDGCHC 7 cut(s) 67, 142, 153, 209, 389, 829, 1578
SexAI ACCWGGT 2 cut(s) 655, 1532
SfaNI GCATC 7 cut(s) 174, 271, 335, 766, 826, 874, 1969
SfcI CTRYAG 2 cut(s) 806, 1938
Sfr274I CTCGAG 1 cut(s) 752
SfuI TTCGAA 3 cut(s) 81, 100, 1028
SinI GGWCC 2 cut(s) 1361, 1603
SlaI CTCGAG 1 cut(s) 752
SmiMI CAYNNNNRTG 1 cut(s) 1486
SmlI CTYRAG 5 cut(s) 200, 420, 635, 752, 1825
SmoI CTYRAG 5 cut(s) 200, 420, 635, 752, 1825
SpeI ACTAGT 2 cut(s) 401, 1460
Sse9I AATT 7 cut(s) 286, 310, 505, 1208, 1310, 1786, 1881
SseBI AGGCCT 1 cut(s) 1836
SspMI CTAG 5 cut(s) 402, 444, 873, 1461, 1902
SstI GAGCTC 2 cut(s) 389, 1578
StuI AGGCCT 1 cut(s) 1836
StyD4I CCNGG 7 cut(s) 59, 87, 655, 783, 1175, 1532, 1836
StyI CCWWGG 2 cut(s) 186, 1125
TaaI ACNGT 5 cut(s) 97, 516, 705, 964, 1990
TaiI ACGT 1 cut(s) 1947
TaqI TCGA 7 cut(s) 81, 100, 477, 746, 753, 829, 1028
TasI AATT 7 cut(s) 286, 310, 505, 1208, 1310, 1786, 1881
TatI WGTACW 1 cut(s) 1813
TauI GCSGC 4 cut(s) 59, 307, 323, 326
TfiI GAWTC 7 cut(s) 78, 686, 929, 1055, 1183, 1389, 1807
Tru1I TTAA 8 cut(s) 636, 954, 966, 1233, 1280, 1632, 1718, 1779
Tru9I TTAA 8 cut(s) 636, 954, 966, 1233, 1280, 1632, 1718, 1779
TscAI CASTG 3 cut(s) 1629, 1746, 1995
TseFI GTSAC 1 cut(s) 497
TseI GCWGC 6 cut(s) 805, 808, 1087, 1421, 1683, 1868
Tsp45I GTSAC 1 cut(s) 497
TspDTI ATGAA 5 cut(s) 303, 1221, 1595, 1676, 1786
TspGWI ACGGA 2 cut(s) 348, 1607
TspRI CASTG 3 cut(s) 1629, 1746, 1995
Tth111I GACNNNGTC 1 cut(s) 221
Van91I CCANNNNNTGG 2 cut(s) 1000, 1162
Vha464I CTTAAG 1 cut(s) 635
VpaK11BI GGWCC 2 cut(s) 1361, 1603
VspI ATTAAT 1 cut(s) 1280
XagI CCTNNNNNAGG 2 cut(s) 782, 1841
XapI RAATTY 1 cut(s) 286
XceI RCATGY 1 cut(s) 1960
XhoI CTCGAG 1 cut(s) 752
XmiI GTMKAC 1 cut(s) 1294
XspI CTAG 5 cut(s) 402, 444, 873, 1461, 1902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.