MD01G1185300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001384
Physical Location & Seq
Reverse (-)
28436229 .. 28444097
7869 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1185300.v1.1.491

Sequence Viewer

Length: 2340 bp
ATGGCTTCTACAAACTCAACCCCCACCGACCCATCTCTCTCGCCGGACGACATAGCCGCTAGGGCCCTCAACAGGCGATTCGAAAGCTTGGTGACTGTTCGAACAAAGGCCATAAAGGGGAAAGGAGCTTGGTACTGGGCTCACTTGGAGCCCATTCTCGTTCGCACCCCCAACTCCAACGTCCCCAAGGCCGTCAAGCTCAAGTGCTCCCTCTGCGACGCCGTCTTCTCCGCCTCCAACCCCTCCAGAACCGCCTCGGAGCATCTCAAACGGGGTACCTGCCCGAATTTTACCTCTGTTTTGAGACCCAATTCAACGGTGGCGGCTTCGCCGGTTCCCATATCTTCCTTGCCGTCTCCGTCGTCACTCAATCACAGAAAGCGGAGCTCGCAAATGGGTATTAGTCCTTCTCCTATTTCTCAAGCTCCTCCTCTTACTAGTCCTACGTCGATTCAGGTTCATTCTTTAGCTATGATCGAGTCTTCCGGCTTCTGCGGTGATCATAATTTCTCACAGTCTCCAAACCCAGTTGGGATTGCGAGAAGTACGGGGCTAGATCAGCACCATTTGGTGTTATCCGGTGGGAAGGAGGATTTGGGTGCTTTGGCAATGTTAGAAAAAAGTGTGAAGAAACTTAAGAGTCCCAAAGCTTCGCCTGGTGCCACTCTTAATAAGGAGCAGATTGATTCTGCACTTGAATTACTGTCTGAGTGGTTCTATGAGTCATGTGGGTCGGTCTCGTTTTCGAGCCTCGAGCATCCCAAGTTCAGGGCTTTCCTTAGCCAGGTGGGCTTGCCTGCATTGTTGCAGCGCGAGCTTTCGGGTGCTCGACTTGATGCTAAGTTTGATGAGGTGAAAGCTGAGTCTGAAGCTAGGATTAGAGATGCAATGTTTTTTCAAGTTGCTTCTGATGGGTGGAAAAGCAGGGATTCTTGTGGGGAGGAGAATGTTGTTAAGTTCACGGTTAATCTTCCGAATGGGATTAGTGTTTTCCAGAAGGCAGTATTTACTGGCGGGTCAGTTCCGACGAAATATGCAGAAGAGGTGTTGTGGGACTCAGTTACCACTATATGTGGGAATGCTGTGCAGCGTTGCGTGGGGATAGTTGCGGACAAGTATAAGGCCAAGGCACTGAGGAATTTGGAGACTCAGAACCATTGGATGGTGAACGTCTCCTGTCAGCTTCAGGGGTTCATGTCTTTGATTAAGGATTTTAACAAAGAGCTTCCATTGTTCAGGGTTGTCATTGAAAATTGCTTAAAGGTTGCGAATTTTGTAGATAGCACCTCTGAGGTTAGGCATGCTTTCGAGAAGTACAAGATGCAAGAGCTTGAATATGCTGGGTTGATCCAAATTCCTTCGCCCAAATGTGATACCTCAAAGAACTTTGCACCTGTCTATGCAATGCTGGAGGATATATTGAGCTGTGCCCGCATACTCCAAATGGTTGTCTTGGATGATTTCTATAAGGCGATATGTCTGGAGGATCCAATTGCAAGGGAAGTTGCAGGGATGATTCAAACTGAAGGTTTTTGGAATGAATTAGAGGCAGTTTATTCACTTGTTAAGATGATCAGGGGGATGACTCAGGAGATTGAGGCTGAGAGGCCATTACTTGGGCAATGCCTGCCCCTTTGGGAAGAATTGAGAACAAAAGTAAAGGATTGGTGTGCCAAATTCAACATTGTCGAAGGTCCTGTTGAGAAAATAGTTGAAAGACGATTCAGAAAGAACTACCACCCAGCATGGTCGGCTGCATTTATACTTGACCCGATATACTTGATGAGGGACACTAGTGGAAAGTACCTTCCACCATTCAAGTGCTTGACGCACGAGCAGGAGAAGGATGTAGATAAGCTCATTACGAGGTTGGTTTCCAGGGAAGAAGCTCATGTTGCATTGATGGAGCTCATGAAATGGAGAACAGAAGGAATGGACCCGCTGTATGCTCAGGCAGTTCAGGTTAAACAGCGAGATCCTGTAACTGGAAAGATGAAAATGGCAAACCCGCAGAGCAGCAGACTTGTGTGGGAAACTTGTCTAAGTGAGTTAAAGACATTGGGGAGGGTTGCAGTGCGACTTATCTTCCTCCATGCAACCTCATGTGGATTTAAGTGCAATTGGTCTTTCATGAAATGGATTCGTGTACATAGGCACTCAAGGGTAGGCCTGGAAAAGGTGCAGAAGATGATATTTATTGCAGCTCATGCCAAACTCGAGAGAAGGGATCTTTCTAGTGAGGAAGAAAAGGAAGCAGAGCTGTTTGGGACTGCAGACATTGAGGATGACATGCTGACTGAGGTCTTTTCCGGTGCACCCACAGTGACAGGGTTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

780

Amino Acids

86.98

Weight (kDa)

8.37

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7963 PF25908 16 - 98 3.1e-45 Domain of unknown function (DUF7963)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 287
Acc65I GGTACC 1 cut(s) 275
AccB1I GGYRCC 2 cut(s) 275, 659
AccB7I CCANNNNNTGG 2 cut(s) 1162, 1616
AccII CGCG 1 cut(s) 813
AclWI GGATC 5 cut(s) 1342, 1481, 1494, 1970, 2235
AcsI RAATTY 5 cut(s) 286, 1138, 1270, 1353, 1676
AcuI CTGAAG 3 cut(s) 888, 1169, 1545
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 6 cut(s) 134, 277, 547, 1316, 1805, 2148
AfiI CCNNNNNNNGG 8 cut(s) 72, 117, 768, 784, 1162, 1616, 1985, 2176
AflII CTTAAG 1 cut(s) 635
AgsI TTSAA 9 cut(s) 315, 698, 899, 1250, 1334, 1520, 1681, 1715, 1819
AhlI ACTAGT 2 cut(s) 437, 1793
AjnI CCWGG 4 cut(s) 655, 783, 1877, 2169
AjuI GAANNNNNNNTTGG 2 cut(s) 578, 610
Alw21I GWGCWC 5 cut(s) 209, 389, 829, 1911, 2317
Alw26I GTCTC 6 cut(s) 298, 360, 522, 742, 1139, 1177
Alw44I GTGCAC 1 cut(s) 2313
AlwI GGATC 5 cut(s) 1342, 1481, 1494, 1970, 2235
Ama87I CYCGRG 2 cut(s) 752, 2216
AoxI GGCC 6 cut(s) 63, 108, 189, 1122, 1607, 2167
ApaI GGGCCC 1 cut(s) 67
ApaLI GTGCAC 1 cut(s) 2313
ApeKI GCWGC 5 cut(s) 808, 1087, 1754, 2016, 2201
ApoI RAATTY 5 cut(s) 286, 1138, 1270, 1353, 1676
Asp718I GGTACC 1 cut(s) 275
AspLEI GCGC 1 cut(s) 813
AspS9I GGNCC 4 cut(s) 63, 64, 1694, 1936
AsuHPI GGTGA 4 cut(s) 103, 509, 865, 1177
AsuII TTCGAA 2 cut(s) 81, 100
AvaI CYCGRG 2 cut(s) 752, 2216
AvaII GGWCC 2 cut(s) 1694, 1936
BaeGI GKGCMC 3 cut(s) 67, 1432, 2317
BamHI GGATCC 1 cut(s) 1486
BanI GGYRCC 2 cut(s) 275, 659
BanII GRGCYC 5 cut(s) 67, 142, 153, 389, 1911
BarI GAAGNNNNNNTAC 2 cut(s) 391, 423
BauI CACGAG 1 cut(s) 1832
BbsI GAAGAC 2 cut(s) 217, 474
Bbv12I GWGCWC 5 cut(s) 209, 389, 829, 1911, 2317
BbvI GCAGC 5 cut(s) 820, 1099, 1741, 2028, 2213
BccI CCATC 4 cut(s) 40, 905, 1156, 1897
BceAI ACGGC 3 cut(s) 176, 206, 337
BciT130I CCWGG 4 cut(s) 657, 785, 1879, 2171
BclI TGATCA 2 cut(s) 499, 1572
BcoDI GTCTC 6 cut(s) 298, 360, 522, 742, 1139, 1177
BcuI ACTAGT 2 cut(s) 437, 1793
BfaI CTAG 6 cut(s) 60, 438, 554, 873, 1794, 2235
BfmI CTRYAG 1 cut(s) 2271
BfrI CTTAAG 1 cut(s) 635
BfuAI ACCTGC 1 cut(s) 287
BglI GCCNNNNNGGC 2 cut(s) 62, 789
BisI GCNGC 7 cut(s) 57, 324, 809, 1088, 1755, 2017, 2202
BlsI GCNGC 7 cut(s) 58, 325, 810, 1089, 1756, 2018, 2203
Bme1390I CCNGG 4 cut(s) 657, 785, 1879, 2171
Bme18I GGWCC 2 cut(s) 1694, 1936
BmeT110I CYCGRG 2 cut(s) 752, 2216
BmgT120I GGNCC 4 cut(s) 63, 64, 1694, 1936
BmiI GGNNCC 7 cut(s) 65, 150, 277, 336, 661, 1488, 1938
BmrFI CCNGG 4 cut(s) 657, 785, 1879, 2171
BmrI ACTGGG 2 cut(s) 145, 521
BmsI GCATC 5 cut(s) 271, 766, 826, 874, 1311
BmuI ACTGGG 2 cut(s) 145, 521
BoxI GACNNNNGTC 1 cut(s) 2300
BpiI GAAGAC 2 cut(s) 217, 474
BpmI CTGGAG 3 cut(s) 229, 1430, 1502
Bpu10I CCTNAGC 2 cut(s) 779, 1950
Bpu14I TTCGAA 2 cut(s) 81, 100
BpuEI CTTGAG 3 cut(s) 185, 405, 2143
BsaHI GRCGYC 1 cut(s) 219
BsaI GGTCTC 2 cut(s) 298, 742
BsaJI CCNNGG 4 cut(s) 186, 255, 1125, 1878
BsaWI WCCGGW 2 cut(s) 578, 2309
Bsc4I CCNNNNNNNGG 8 cut(s) 72, 117, 768, 784, 1162, 1616, 1985, 2176
Bse118I RCCGGY 1 cut(s) 331
Bse1I ACTGG 4 cut(s) 140, 527, 1015, 1990
Bse3DI GCAATG 4 cut(s) 615, 894, 1410, 1628
BseBI CCWGG 4 cut(s) 657, 785, 1879, 2171
BseDI CCNNGG 4 cut(s) 186, 255, 1125, 1878
BseGI GGATG 7 cut(s) 757, 1167, 1462, 1518, 1587, 1852, 2290
BseLI CCNNNNNNNGG 8 cut(s) 72, 117, 768, 784, 1162, 1616, 1985, 2176
BseMI GCAATG 4 cut(s) 615, 894, 1410, 1628
BseNI ACTGG 4 cut(s) 140, 527, 1015, 1990
BseRI GAGGAG 3 cut(s) 417, 420, 956
BseSI GKGCMC 3 cut(s) 67, 1432, 2317
BseXI GCAGC 5 cut(s) 820, 1099, 1741, 2028, 2213
BseYI CCCAGC 2 cut(s) 1340, 1741
BsgI GTGCAG 3 cut(s) 675, 1106, 2201
Bsh1236I CGCG 1 cut(s) 813
BshFI GGCC 6 cut(s) 65, 110, 191, 1124, 1609, 2169
BshNI GGYRCC 2 cut(s) 275, 659
BsiHKAI GWGCWC 5 cut(s) 209, 389, 829, 1911, 2317
BsiHKCI CYCGRG 2 cut(s) 752, 2216
BsiSI CCGG 5 cut(s) 44, 332, 486, 579, 2310
BslFI GGGAC 5 cut(s) 167, 627, 1067, 1802, 2281
BslI CCNNNNNNNGG 8 cut(s) 72, 117, 768, 784, 1162, 1616, 1985, 2176
BsmAI GTCTC 6 cut(s) 298, 360, 522, 742, 1139, 1177
BsmBI CGTCTC 2 cut(s) 360, 1177
BsmFI GGGAC 5 cut(s) 167, 627, 1067, 1802, 2281
BsmI GAATGC 1 cut(s) 1084
BsnI GGCC 6 cut(s) 65, 110, 191, 1124, 1609, 2169
Bso31I GGTCTC 2 cut(s) 298, 742
BsoBI CYCGRG 2 cut(s) 752, 2216
Bsp119I TTCGAA 2 cut(s) 81, 100
Bsp120I GGGCCC 1 cut(s) 63
Bsp1286I GDGCHC 9 cut(s) 67, 142, 153, 209, 389, 829, 1432, 1911, 2317
Bsp1407I TGTACA 1 cut(s) 2146
Bsp143I GATC 8 cut(s) 474, 499, 556, 1347, 1486, 1572, 1975, 2227
BspANI GGCC 6 cut(s) 65, 110, 191, 1124, 1609, 2169
BspFNI CGCG 1 cut(s) 813
BspHI TCATGA 2 cut(s) 1911, 2130
BspLI GGNNCC 7 cut(s) 65, 150, 277, 336, 661, 1488, 1938
BspMAI CTGCAG 1 cut(s) 2275
BspMI ACCTGC 1 cut(s) 287
BspPI GGATC 5 cut(s) 1342, 1481, 1494, 1970, 2235
BspT104I TTCGAA 2 cut(s) 81, 100
BspT107I GGYRCC 2 cut(s) 275, 659
BspTI CTTAAG 1 cut(s) 635
BspTNI GGTCTC 2 cut(s) 298, 742
BsrDI GCAATG 4 cut(s) 615, 894, 1410, 1628
BsrFI RCCGGY 1 cut(s) 331
BsrGI TGTACA 1 cut(s) 2146
BsrI ACTGG 4 cut(s) 140, 527, 1015, 1990
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 4 cut(s) 186, 255, 1125, 1878
BssMI GATC 8 cut(s) 474, 499, 556, 1347, 1486, 1572, 1975, 2227
BssNI GRCGYC 1 cut(s) 219
BssSI CACGAG 1 cut(s) 1832
BssT1I CCWWGG 2 cut(s) 186, 1125
Bst2BI CACGAG 1 cut(s) 1832
Bst2UI CCWGG 4 cut(s) 657, 785, 1879, 2171
Bst4CI ACNGT 6 cut(s) 97, 319, 516, 705, 964, 2323
Bst6I CTCTTC 1 cut(s) 1035
BstACI GRCGYC 1 cut(s) 219
BstAFI CTTAAG 1 cut(s) 635
BstAPI GCANNNNNTGC 2 cut(s) 1627, 2207
BstAUI TGTACA 1 cut(s) 2146
BstBI TTCGAA 2 cut(s) 81, 100
BstC8I GCNNGC 7 cut(s) 389, 794, 798, 815, 1302, 1432, 1628
BstENI CCTNNNNNAGG 2 cut(s) 782, 2174
BstF5I GGATG 7 cut(s) 757, 1167, 1462, 1518, 1587, 1852, 2290
BstFNI CGCG 1 cut(s) 813
BstHHI GCGC 1 cut(s) 813
BstKTI GATC 8 cut(s) 477, 502, 559, 1350, 1489, 1575, 1978, 2230
BstMAI GTCTC 6 cut(s) 298, 360, 522, 742, 1139, 1177
BstMBI GATC 8 cut(s) 474, 499, 556, 1347, 1486, 1572, 1975, 2227
BstNI CCWGG 4 cut(s) 657, 785, 1879, 2171
BstNSI RCATGY 2 cut(s) 1304, 2293
BstPAI GACNNNNGTC 1 cut(s) 2300
BstSCI CCNGG 4 cut(s) 655, 783, 1877, 2169
BstSFI CTRYAG 1 cut(s) 2271
BstSLI GKGCMC 3 cut(s) 67, 1432, 2317
BstUI CGCG 1 cut(s) 813
BstV1I GCAGC 5 cut(s) 820, 1099, 1741, 2028, 2213
BstV2I GAAGAC 2 cut(s) 217, 474
BstX2I RGATCY 3 cut(s) 1486, 1975, 2227
BstYI RGATCY 3 cut(s) 1486, 1975, 2227
BsuRI GGCC 6 cut(s) 65, 110, 191, 1124, 1609, 2169
BtsCI GGATG 7 cut(s) 757, 1167, 1462, 1518, 1587, 1852, 2290
BtsI GCAGTG 1 cut(s) 2079
BtsIMutI CAGTG 3 cut(s) 1130, 2079, 2328
BveI ACCTGC 1 cut(s) 287
Cac8I GCNNGC 7 cut(s) 389, 794, 798, 815, 1302, 1432, 1628
CciI TCATGA 2 cut(s) 1911, 2130
CfoI GCGC 1 cut(s) 813
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 4 cut(s) 63, 64, 1694, 1936
CseI GACGC 2 cut(s) 227, 1837
Csp6I GTAC 6 cut(s) 133, 276, 546, 1315, 1804, 2147
CviQI GTAC 6 cut(s) 133, 276, 546, 1315, 1804, 2147
DpnI GATC 8 cut(s) 476, 501, 558, 1349, 1488, 1574, 1977, 2229
DpnII GATC 8 cut(s) 474, 499, 556, 1347, 1486, 1572, 1975, 2227
Eam1104I CTCTTC 1 cut(s) 1035
EarI CTCTTC 1 cut(s) 1035
EciI GGCGGA 1 cut(s) 220
Ecl136II GAGCTC 2 cut(s) 387, 1909
Eco130I CCWWGG 2 cut(s) 186, 1125
Eco147I AGGCCT 1 cut(s) 2169
Eco24I GRGCYC 5 cut(s) 67, 142, 153, 389, 1911
Eco31I GGTCTC 2 cut(s) 298, 742
Eco47I GGWCC 2 cut(s) 1694, 1936
Eco53kI GAGCTC 2 cut(s) 387, 1909
Eco57I CTGAAG 3 cut(s) 888, 1169, 1545
Eco88I CYCGRG 2 cut(s) 752, 2216
EcoICRI GAGCTC 2 cut(s) 387, 1909
EcoNI CCTNNNNNAGG 2 cut(s) 782, 2174
EcoO109I RGGNCCY 3 cut(s) 63, 64, 1694
EcoRII CCWGG 4 cut(s) 655, 783, 1877, 2169
EcoT14I CCWWGG 2 cut(s) 186, 1125
EcoT38I GRGCYC 5 cut(s) 67, 142, 153, 389, 1911
ErhI CCWWGG 2 cut(s) 186, 1125
Esp3I CGTCTC 2 cut(s) 360, 1177
FaqI GGGAC 5 cut(s) 167, 627, 1067, 1802, 2281
FauI CCCGC 4 cut(s) 1007, 1439, 1947, 2016
FbaI TGATCA 2 cut(s) 499, 1572
Fnu4HI GCNGC 7 cut(s) 57, 324, 809, 1088, 1755, 2017, 2202
FokI GGATG 7 cut(s) 744, 1174, 1469, 1525, 1594, 1859, 2297
FriOI GRGCYC 5 cut(s) 67, 142, 153, 389, 1911
Fsp4HI GCNGC 7 cut(s) 57, 324, 809, 1088, 1755, 2017, 2202
FspBI CTAG 6 cut(s) 60, 438, 554, 873, 1794, 2235
GlaI GCGC 1 cut(s) 812
GluI GCNGC 7 cut(s) 57, 324, 809, 1088, 1755, 2017, 2202
GsaI CCCAGC 2 cut(s) 1344, 1745
GsuI CTGGAG 3 cut(s) 229, 1430, 1502
HaeIII GGCC 6 cut(s) 65, 110, 191, 1124, 1609, 2169
HapII CCGG 5 cut(s) 44, 332, 486, 579, 2310
HgaI GACGC 2 cut(s) 227, 1837
HhaI GCGC 1 cut(s) 813
Hin1I GRCGYC 1 cut(s) 219
Hin6I GCGC 1 cut(s) 811
HinP1I GCGC 1 cut(s) 811
HindIII AAGCTT 2 cut(s) 85, 648
HpaII CCGG 5 cut(s) 44, 332, 486, 579, 2310
HphI GGTGA 4 cut(s) 103, 509, 865, 1177
Hpy166II GTNNAC 4 cut(s) 960, 1168, 2147, 2315
Hpy188I TCNGA 9 cut(s) 259, 709, 868, 910, 975, 1026, 1152, 1291, 1727
Hpy188III TCNNGA 9 cut(s) 246, 994, 1309, 1481, 1589, 1912, 2131, 2218, 2337
Hpy8I GTNNAC 4 cut(s) 960, 1168, 2147, 2315
Hpy99I CGWCG 4 cut(s) 221, 364, 451, 1030
HpyCH4III ACNGT 6 cut(s) 97, 319, 516, 705, 964, 2323
HpyCH4IV ACGT 3 cut(s) 180, 446, 1170
HpySE526I ACGT 3 cut(s) 180, 446, 1170
Hsp92I GRCGYC 1 cut(s) 219
HspAI GCGC 1 cut(s) 811
KpnI GGTACC 1 cut(s) 279
Ksp22I TGATCA 2 cut(s) 499, 1572
Kzo9I GATC 8 cut(s) 474, 499, 556, 1347, 1486, 1572, 1975, 2227
LmnI GCTCC 8 cut(s) 125, 148, 212, 259, 384, 430, 676, 1906
Lsp1109I GCAGC 5 cut(s) 820, 1099, 1741, 2028, 2213
LweI GCATC 5 cut(s) 271, 766, 826, 874, 1311
MaeI CTAG 6 cut(s) 60, 438, 554, 873, 1794, 2235
MaeII ACGT 3 cut(s) 180, 446, 1170
MaeIII GTNAC 5 cut(s) 91, 363, 1060, 1981, 2323
MalI GATC 8 cut(s) 476, 501, 558, 1349, 1488, 1574, 1977, 2229
MboI GATC 8 cut(s) 474, 499, 556, 1347, 1486, 1572, 1975, 2227
MfeI CAATTG 2 cut(s) 1491, 2119
MflI RGATCY 3 cut(s) 1486, 1975, 2227
MhlI GDGCHC 9 cut(s) 67, 142, 153, 209, 389, 829, 1432, 1911, 2317
MlyI GAGTC 7 cut(s) 488, 649, 731, 872, 1049, 1141, 1579
MmeI TCCRAC 3 cut(s) 201, 261, 1049
MslI CAYNNNNRTG 1 cut(s) 1819
MspA1I CMGCKG 1 cut(s) 1942
MspCI CTTAAG 1 cut(s) 635
MspI CCGG 5 cut(s) 44, 332, 486, 579, 2310
MspR9I CCNGG 4 cut(s) 657, 785, 1879, 2171
MunI CAATTG 2 cut(s) 1491, 2119
Mva1269I GAATGC 1 cut(s) 1084
MvaI CCWGG 4 cut(s) 657, 785, 1879, 2171
MvnI CGCG 1 cut(s) 813
NdeII GATC 8 cut(s) 474, 499, 556, 1347, 1486, 1572, 1975, 2227
NlaIV GGNNCC 7 cut(s) 65, 150, 277, 336, 661, 1488, 1938
NmuCI GTSAC 3 cut(s) 91, 363, 2323
NspI RCATGY 2 cut(s) 1304, 2293
NspV TTCGAA 2 cut(s) 81, 100
PaeI GCATGC 1 cut(s) 1304
PaeR7I CTCGAG 2 cut(s) 752, 2216
PagI TCATGA 2 cut(s) 1911, 2130
PceI AGGCCT 1 cut(s) 2169
PctI GAATGC 1 cut(s) 1084
PfeI GAWTC 7 cut(s) 78, 451, 686, 929, 1516, 1722, 2140
PflFI GACNNNGTC 1 cut(s) 221
PflMI CCANNNNNTGG 2 cut(s) 1162, 1616
PkrI GCNGC 7 cut(s) 58, 325, 810, 1089, 1756, 2018, 2203
PleI GAGTC 7 cut(s) 487, 648, 730, 871, 1049, 1141, 1579
PpsI GAGTC 7 cut(s) 487, 648, 730, 871, 1049, 1141, 1579
PpuMI RGGWCCY 1 cut(s) 1694
PshAI GACNNNNGTC 1 cut(s) 2300
Psp124BI GAGCTC 2 cut(s) 389, 1911
Psp5II RGGWCCY 1 cut(s) 1694
Psp6I CCWGG 4 cut(s) 655, 783, 1877, 2169
PspFI CCCAGC 2 cut(s) 1340, 1741
PspGI CCWGG 4 cut(s) 655, 783, 1877, 2169
PspN4I GGNNCC 7 cut(s) 65, 150, 277, 336, 661, 1488, 1938
PspOMI GGGCCC 1 cut(s) 63
PspPI GGNCC 4 cut(s) 63, 64, 1694, 1936
PspPPI RGGWCCY 1 cut(s) 1694
PspXI VCTCGAGB 1 cut(s) 752
PstI CTGCAG 1 cut(s) 2275
PsuI RGATCY 3 cut(s) 1486, 1975, 2227
PsyI GACNNNGTC 1 cut(s) 221
RsaI GTAC 6 cut(s) 134, 277, 547, 1316, 1805, 2148
RsaNI GTAC 6 cut(s) 133, 276, 546, 1315, 1804, 2147
RseI CAYNNNNRTG 1 cut(s) 1819
SacI GAGCTC 2 cut(s) 389, 1911
SatI GCNGC 7 cut(s) 57, 324, 809, 1088, 1755, 2017, 2202
Sau3AI GATC 8 cut(s) 474, 499, 556, 1347, 1486, 1572, 1975, 2227
Sau96I GGNCC 4 cut(s) 63, 64, 1694, 1936
SchI GAGTC 7 cut(s) 488, 649, 731, 872, 1049, 1141, 1579
ScrFI CCNGG 4 cut(s) 657, 785, 1879, 2171
SduI GDGCHC 9 cut(s) 67, 142, 153, 209, 389, 829, 1432, 1911, 2317
SfaNI GCATC 5 cut(s) 271, 766, 826, 874, 1311
SfcI CTRYAG 1 cut(s) 2271
Sfr274I CTCGAG 2 cut(s) 752, 2216
SfuI TTCGAA 2 cut(s) 81, 100
SinI GGWCC 2 cut(s) 1694, 1936
SlaI CTCGAG 2 cut(s) 752, 2216
SmiMI CAYNNNNRTG 1 cut(s) 1819
SmlI CTYRAG 6 cut(s) 200, 420, 635, 752, 2158, 2216
SmoI CTYRAG 6 cut(s) 200, 420, 635, 752, 2158, 2216
SpeI ACTAGT 2 cut(s) 437, 1793
SphI GCATGC 1 cut(s) 1304
SseBI AGGCCT 1 cut(s) 2169
SspMI CTAG 6 cut(s) 60, 438, 554, 873, 1794, 2235
SstI GAGCTC 2 cut(s) 389, 1911
StuI AGGCCT 1 cut(s) 2169
StyD4I CCNGG 4 cut(s) 655, 783, 1877, 2169
StyI CCWWGG 2 cut(s) 186, 1125
TaaI ACNGT 6 cut(s) 97, 319, 516, 705, 964, 2323
TaiI ACGT 3 cut(s) 183, 449, 1173
TaqII GACCGA 1 cut(s) 724
TatI WGTACW 2 cut(s) 1314, 2146
TauI GCSGC 2 cut(s) 59, 326
TfiI GAWTC 7 cut(s) 78, 451, 686, 929, 1516, 1722, 2140
TscAI CASTG 3 cut(s) 1137, 2079, 2328
TseFI GTSAC 3 cut(s) 91, 363, 2323
TseI GCWGC 5 cut(s) 808, 1087, 1754, 2016, 2201
Tsp45I GTSAC 3 cut(s) 91, 363, 2323
TspDTI ATGAA 7 cut(s) 449, 1183, 1554, 1928, 2009, 2119, 2147
TspGWI ACGGA 1 cut(s) 348
TspRI CASTG 3 cut(s) 1137, 2079, 2328
Tth111I GACNNNGTC 1 cut(s) 221
Van91I CCANNNNNTGG 2 cut(s) 1162, 1616
Vha464I CTTAAG 1 cut(s) 635
VneI GTGCAC 1 cut(s) 2313
VpaK11BI GGWCC 2 cut(s) 1694, 1936
XagI CCTNNNNNAGG 2 cut(s) 782, 2174
XapI RAATTY 5 cut(s) 286, 1138, 1270, 1353, 1676
XceI RCATGY 2 cut(s) 1304, 2293
XcmI CCANNNNNNNNNTGG 1 cut(s) 316
XhoI CTCGAG 2 cut(s) 752, 2216
XspI CTAG 6 cut(s) 60, 438, 554, 873, 1794, 2235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.