FvH4_7g28141

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001384
Physical Location & Seq
Reverse (-)
20900657 .. 20905440
4784 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g28141.t1

Sequence Viewer

Length: 2343 bp
ATGGACTCAACAAACTCCACCCCAGCCGACCCAGCTCCTCTCTCGCCGGACGACGCGGCGGCGAGAGCGCTGAACAAACGCTACGAGGGTCTCGTAACTGTTCGAACAAAGGCCATAAAGGGCAAAGGAGCTTGGTACTGGGCTCACTTGGAGCCTGTCCTGGTCCGCAACACCACCACCAACCTTCCCAAAGCTGTAAAGCTCAAGTGTCTTCTCTGCGACGCCGTTTTCTCCGCCTCAAACCCTTCCAGAACCGCCTCCGAGCACCTCAAGCGCGGCACGTGTCCGAATTTCACCTCCGTCTTGCGACCCATTTCGTCGGCGTCGCTCTCGCCGCAGCCCATCTCGTCTCTGCCGTCGCCGTCGTCACACAACCACAGAAAACGAAGCTCGGAAATGGGTACTCTATTCTCTCCTATATCTCAGGCCCCCACCTCTACTTCGATTCAAGTACATTCTTTGGCTATGATCGAGGCCCCCGGTTTTTGCGGCGACATTTACTCACACAACCCGAACCCGGGTCCGGGTCCGAGCCCGACCCCGGTCGGGTTGAGCCAGCAGCACCATTTGGTGTTGTCTGGTGGGAAAGACGACTTGGGTGCTTTGGCAATGTTGGAGAATAGTGTCAAGAAGCTTAAAAGCCCCAAGCCTTCTTCTGTTGGTGTTTCATTGACTAAGGAGCAGGTTGATTCTGCGCTTGAGTTGTTGTCTGAGTGGTTCTATGAGTCATGTGGGTCTGTCTCGTTTTCGAGCCTCGAGCATCCCAAGTTCAAGGCCTTTCTTGGCCAGGTGGGCTTGCCGGCGGTGTTGCAAAGGGAGCTTTCGGGTGCTAGGCTTGATGCTAAGTATGCCAAGGCCAAAGCTGAATGTGAAGCTAGGATAAGGGATGCAATGTTTTTTCAGGTGGCTTGTGATGGTTGGGAGAGTAATAGTTTTTGTGGTGGGGAGGAGAGTATGGTTAAGTTTATGGTTAATCTTCCGAATGGGAGTAGTGTGTTTCAGAAGGCTGTATTTACTGCAGGGTCAGTCTCGTCGAAATATGCTGAGGAGGTAATGTGGGATGCGGTGAGTGGCGTGTGTGGGAATGCTATGCAGCGTTGTGTGGGGATTGTGGCTGATAAGTATAAAGCCAAGGCGTTGAGGAATTTGGAGAGTCAGAATCATTGGATGGTGAATGTTTCGTGTCAGCTACAGGGGTTTATTTCTTTGATCAAGGATTTTCACAAAGAACATAAATTGTTTATGGTTGTTACTGATAATTGCTTAAAGGTTGCCAATTTTGTAAATACCACCACCGAGGTTAGGCGGATTTTCCAGAAGTACAAGATGCAGAAGCTTGGGTATGCTGGGTTGCTCCAAGTTTCTTCTTCCAAATGCGATAGTTCAAAAGACTATGCACTTGTTTATGCAATGCTGGAGGATATGTTGACCTGTTCCAGCATACTTGAAATGGTGGTATTGGATGATTGCTATAAGGAAACATGTGAGGAGGATTCAGTTGCTAGGGAAGTTGCAGTGATGATTCAAAATGTAGGATTCTGGAATGAACTAGGGGCAGTTTATTCATTTATGAAGCTGATAAGAGGGATGACTCAGGAGATTGAAGCTGAGAGGCCATTAATTGGGCAATGCCTACCCCTTTGGGAAGAGTTGCGAACAAAAGTGAAGGGATGGTGTGCCAAGTTTAACATTGATGAAGGACCTGTTGAGAAAATAGTTGAAAAACGGTTCGCAAAGAATTATCACCCAGCATGGTCAGCTGCATTTATACTTGATCCACAATACTTGATGAGGGACACTAGTGGAAAGTACCTTCCACCATTCAAGTGCTTGACGCATGAGCAGGAGAAGGATGTAGATAAGCTCATTACCAGATTGGTATCCAGGGAAGAAGCGCATGTTGCATTAATGGAGCTGATGAAATGGAGGACAGAAGGAATGGATCCGCTCTATGCTCAGGCAGTTCAGGTTAAACAGCAAGATCCTGTAACTGGGAAAATGAAAATTGCAAACCCGCAGAGCAGTCGACTTGTGTGGGAAACTTGTCTAAGCAAATTAAACATACTGGGGAAGGTTGCAGTGAGACTTATCTTCCTCCATGCAACGTCATTTGGATTTAAGTGCAATTGGTCTTTCATGAAATGGATTTGTGTACATAGGCACTCAAGGATAGGCCTGGAAAGGGCAGAGAAGATGATATTCATTGCCGCCCATGCAAAGCTTGAAAGAAGGGATCTCTCTAACGACGAAGAAAGGGAAGCAGAGCTGTTTGCGACTGCAAGTGTTGATGATGAGATGCTGAATGAGGGCCTTTCCGATGCACCCACAACTAGGGTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

781

Amino Acids

86.66

Weight (kDa)

8.2

Isoelectric Point (pI)

42.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7963 PF25908 17 - 99 2.1e-45 Domain of unknown function (DUF7963)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 673
AccB7I CCANNNNNTGG 1 cut(s) 1622
AccBSI CCGCTC 1 cut(s) 1948
AccI GTMKAC 1 cut(s) 2026
AccII CGCG 2 cut(s) 56, 276
AclWI GGATC 5 cut(s) 1769, 1937, 1950, 1976, 2241
AcoI YGGCCR 1 cut(s) 784
AcsI RAATTY 2 cut(s) 289, 1144
AcvI CACGTG 1 cut(s) 282
AcyI GRCGYC 2 cut(s) 222, 323
AfaI GTAC 6 cut(s) 137, 403, 453, 1322, 1811, 2154
AfeI AGCGCT 1 cut(s) 69
AflIII ACRYGT 2 cut(s) 281, 1481
AgsI TTSAA 9 cut(s) 449, 772, 1386, 1448, 1526, 1604, 1721, 1825, 2225
AhlI ACTAGT 1 cut(s) 1799
AjnI CCWGG 4 cut(s) 159, 786, 1883, 2175
AjuI GAANNNNNNNTTGG 2 cut(s) 578, 610
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 5 cut(s) 95, 354, 745, 1033, 2077
AlwI GGATC 5 cut(s) 1769, 1937, 1950, 1976, 2241
Ama87I CYCGRG 2 cut(s) 517, 755
Aor51HI AGCGCT 1 cut(s) 69
AoxI GGCC 9 cut(s) 111, 426, 474, 774, 784, 855, 1613, 2173, 2308
ApeKI GCWGC 4 cut(s) 337, 559, 1093, 1760
ApoI RAATTY 2 cut(s) 289, 1144
AseI ATTAAT 2 cut(s) 1619, 1907
AspLEI GCGC 4 cut(s) 70, 276, 697, 1897
AspS9I GGNCC 7 cut(s) 163, 427, 475, 521, 527, 1700, 2308
AsuC2I CCSGG 5 cut(s) 480, 518, 519, 525, 542
AsuHPI GGTGA 4 cut(s) 286, 1078, 1183, 1736
AsuII TTCGAA 1 cut(s) 103
AvaI CYCGRG 2 cut(s) 517, 755
AvaII GGWCC 4 cut(s) 163, 521, 527, 1700
BalI TGGCCA 1 cut(s) 786
BamHI GGATCC 1 cut(s) 1942
BanII GRGCYC 2 cut(s) 145, 536
BbrPI CACGTG 1 cut(s) 282
BbsI GAAGAC 1 cut(s) 203
Bbv12I GWGCWC 1 cut(s) 267
BbvCI CCTCAGC 1 cut(s) 1044
BbvI GCAGC 4 cut(s) 349, 571, 1105, 1747
BccI CCATC 4 cut(s) 350, 908, 1162, 1665
BceAI ACGGC 3 cut(s) 209, 340, 346
BcgI CGANNNNNNTGC 4 cut(s) 581, 615, 2006, 2040
BciT130I CCWGG 4 cut(s) 161, 788, 1885, 2177
BciVI GTATCC 1 cut(s) 1891
BclI TGATCA 1 cut(s) 1209
BcnI CCSGG 5 cut(s) 480, 518, 519, 525, 542
BcoDI GTCTC 5 cut(s) 95, 354, 745, 1033, 2077
BcuI ACTAGT 1 cut(s) 1799
BfaI CTAG 6 cut(s) 831, 876, 1503, 1550, 1800, 2331
BfmI CTRYAG 2 cut(s) 1017, 1190
BfoI RGCGCY 1 cut(s) 71
BfuAI ACCTGC 1 cut(s) 673
BfuI GTATCC 1 cut(s) 1891
BglI GCCNNNNNGGC 1 cut(s) 792
Bme1390I CCNGG 9 cut(s) 161, 480, 518, 519, 525, 542, 788, 1885, 2177
Bme18I GGWCC 4 cut(s) 163, 521, 527, 1700
BmeT110I CYCGRG 2 cut(s) 517, 755
BmgT120I GGNCC 7 cut(s) 163, 427, 475, 521, 527, 1700, 2308
BmiI GGNNCC 6 cut(s) 153, 429, 477, 522, 528, 1944
BmrFI CCNGG 9 cut(s) 161, 480, 518, 519, 525, 542, 788, 1885, 2177
BmrI ACTGGG 3 cut(s) 148, 2001, 2075
BmsI GCATC 7 cut(s) 769, 829, 877, 1051, 1317, 2286, 2308
BmuI ACTGGG 3 cut(s) 148, 2001, 2075
BoxI GACNNNNGTC 1 cut(s) 542
BpiI GAAGAC 1 cut(s) 203
BpmI CTGGAG 1 cut(s) 1436
Bpu10I CCTNAGC 2 cut(s) 1044, 1956
Bpu14I TTCGAA 1 cut(s) 103
BpuEI CTTGAG 4 cut(s) 188, 254, 719, 2149
BpuMI CCSGG 5 cut(s) 480, 518, 519, 525, 542
BsaAI YACGTR 1 cut(s) 282
BsaBI GATNNNNATC 1 cut(s) 1879
BsaHI GRCGYC 2 cut(s) 222, 323
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 7 cut(s) 478, 517, 540, 852, 1131, 1296, 1884
BsaXI ACNNNNNCTCC 4 cut(s) 1040, 1070, 1409, 1439
Bse118I RCCGGY 1 cut(s) 799
Bse1I ACTGG 3 cut(s) 143, 1996, 2070
Bse3DI GCAATG 5 cut(s) 615, 897, 1416, 1634, 2202
Bse8I GATNNNNATC 1 cut(s) 1879
BseBI CCWGG 4 cut(s) 161, 788, 1885, 2177
BseDI CCNNGG 7 cut(s) 478, 517, 540, 852, 1131, 1296, 1884
BseGI GGATG 8 cut(s) 760, 892, 1066, 1173, 1468, 1593, 1676, 1858
BseJI GATNNNNATC 1 cut(s) 1879
BseMI GCAATG 5 cut(s) 615, 897, 1416, 1634, 2202
BseMII CTCAG 6 cut(s) 437, 702, 1035, 1599, 1607, 1970
BseNI ACTGG 3 cut(s) 143, 1996, 2070
BseRI GAGGAG 4 cut(s) 27, 962, 1061, 1502
BseXI GCAGC 4 cut(s) 349, 571, 1105, 1747
BseYI CCCAGC 4 cut(s) 22, 31, 1346, 1747
Bsh1236I CGCG 2 cut(s) 56, 276
Bsh1285I CGRYCG 1 cut(s) 546
BshFI GGCC 9 cut(s) 113, 428, 476, 776, 786, 857, 1615, 2175, 2310
BsiEI CGRYCG 1 cut(s) 546
BsiHKAI GWGCWC 1 cut(s) 267
BsiHKCI CYCGRG 2 cut(s) 517, 755
BsiSI CCGG 6 cut(s) 47, 480, 518, 524, 542, 800
BslFI GGGAC 1 cut(s) 1808
BsmAI GTCTC 5 cut(s) 95, 354, 745, 1033, 2077
BsmBI CGTCTC 1 cut(s) 354
BsmFI GGGAC 1 cut(s) 1808
BsmI GAATGC 1 cut(s) 1090
BsnI GGCC 9 cut(s) 113, 428, 476, 776, 786, 857, 1615, 2175, 2310
Bso31I GGTCTC 1 cut(s) 95
BsoBI CYCGRG 2 cut(s) 517, 755
Bsp119I TTCGAA 1 cut(s) 103
Bsp1286I GDGCHC 3 cut(s) 145, 267, 536
Bsp1407I TGTACA 1 cut(s) 2152
Bsp143I GATC 6 cut(s) 468, 1209, 1774, 1942, 1981, 2233
BspANI GGCC 9 cut(s) 113, 428, 476, 776, 786, 857, 1615, 2175, 2310
BspCNI CTCAG 6 cut(s) 436, 703, 1036, 1600, 1606, 1969
BspFNI CGCG 2 cut(s) 56, 276
BspHI TCATGA 1 cut(s) 2136
BspLI GGNNCC 6 cut(s) 153, 429, 477, 522, 528, 1944
BspMAI CTGCAG 1 cut(s) 1021
BspMI ACCTGC 1 cut(s) 673
BspPI GGATC 5 cut(s) 1769, 1937, 1950, 1976, 2241
BspT104I TTCGAA 1 cut(s) 103
BspTNI GGTCTC 1 cut(s) 95
BsrBI CCGCTC 1 cut(s) 1948
BsrDI GCAATG 5 cut(s) 615, 897, 1416, 1634, 2202
BsrFI RCCGGY 1 cut(s) 799
BsrGI TGTACA 1 cut(s) 2152
BsrI ACTGG 3 cut(s) 143, 1996, 2070
BssAI RCCGGY 1 cut(s) 799
BssECI CCNNGG 7 cut(s) 478, 517, 540, 852, 1131, 1296, 1884
BssMI GATC 6 cut(s) 468, 1209, 1774, 1942, 1981, 2233
BssNI GRCGYC 2 cut(s) 222, 323
BssT1I CCWWGG 2 cut(s) 852, 1131
Bst2UI CCWGG 4 cut(s) 161, 788, 1885, 2177
Bst4CI ACNGT 2 cut(s) 100, 1728
Bst6I CTCTTC 1 cut(s) 1641
BstACI GRCGYC 2 cut(s) 222, 323
BstAUI TGTACA 1 cut(s) 2152
BstBAI YACGTR 1 cut(s) 282
BstBI TTCGAA 1 cut(s) 103
BstC8I GCNNGC 3 cut(s) 557, 797, 801
BstDEI CTNAG 9 cut(s) 423, 675, 711, 843, 1044, 1593, 1608, 1956, 2048
BstF5I GGATG 8 cut(s) 760, 892, 1066, 1173, 1468, 1593, 1676, 1858
BstFNI CGCG 2 cut(s) 56, 276
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 4 cut(s) 70, 276, 697, 1897
BstKTI GATC 6 cut(s) 471, 1212, 1777, 1945, 1984, 2236
BstMAI GTCTC 5 cut(s) 95, 354, 745, 1033, 2077
BstMBI GATC 6 cut(s) 468, 1209, 1774, 1942, 1981, 2233
BstMCI CGRYCG 1 cut(s) 546
BstNI CCWGG 4 cut(s) 161, 788, 1885, 2177
BstNSI RCATGY 2 cut(s) 1485, 1901
BstPAI GACNNNNGTC 1 cut(s) 542
BstSCI CCNGG 9 cut(s) 159, 478, 516, 517, 523, 540, 786, 1883, 2175
BstSFI CTRYAG 2 cut(s) 1017, 1190
BstUI CGCG 2 cut(s) 56, 276
BstV1I GCAGC 4 cut(s) 349, 571, 1105, 1747
BstV2I GAAGAC 1 cut(s) 203
BstX2I RGATCY 3 cut(s) 1942, 1981, 2233
BstYI RGATCY 3 cut(s) 1942, 1981, 2233
BsuI GTATCC 1 cut(s) 1891
BsuRI GGCC 9 cut(s) 113, 428, 476, 776, 786, 857, 1615, 2175, 2310
BtsCI GGATG 8 cut(s) 760, 892, 1066, 1173, 1468, 1593, 1676, 1858
BtsI GCAGTG 2 cut(s) 1521, 2085
BtsIMutI CAGTG 2 cut(s) 1521, 2085
BveI ACCTGC 1 cut(s) 673
Cac8I GCNNGC 3 cut(s) 557, 797, 801
CciI TCATGA 1 cut(s) 2136
CfoI GCGC 4 cut(s) 70, 276, 697, 1897
Cfr10I RCCGGY 1 cut(s) 799
Cfr13I GGNCC 7 cut(s) 163, 427, 475, 521, 527, 1700, 2308
Cfr9I CCCGGG 1 cut(s) 517
CseI GACGC 4 cut(s) 62, 230, 312, 1843
Csp6I GTAC 6 cut(s) 136, 402, 452, 1321, 1810, 2153
CspCI CAANNNNNGTGG 2 cut(s) 1767, 1802
CviAII CATG 8 cut(s) 729, 1482, 1752, 1838, 1898, 2099, 2137, 2213
CviQI GTAC 6 cut(s) 136, 402, 452, 1321, 1810, 2153
DdeI CTNAG 9 cut(s) 423, 675, 711, 843, 1044, 1593, 1608, 1956, 2048
DpnI GATC 6 cut(s) 470, 1211, 1776, 1944, 1983, 2235
DpnII GATC 6 cut(s) 468, 1209, 1774, 1942, 1981, 2233
EaeI YGGCCR 1 cut(s) 784
Eam1104I CTCTTC 1 cut(s) 1641
EarI CTCTTC 1 cut(s) 1641
EciI GGCGGA 2 cut(s) 223, 1321
Eco130I CCWWGG 2 cut(s) 852, 1131
Eco147I AGGCCT 2 cut(s) 776, 2175
Eco24I GRGCYC 2 cut(s) 145, 536
Eco31I GGTCTC 1 cut(s) 95
Eco47I GGWCC 4 cut(s) 163, 521, 527, 1700
Eco47III AGCGCT 1 cut(s) 69
Eco72I CACGTG 1 cut(s) 282
Eco88I CYCGRG 2 cut(s) 517, 755
EcoO109I RGGNCCY 4 cut(s) 427, 475, 1700, 2308
EcoRII CCWGG 4 cut(s) 159, 786, 1883, 2175
EcoT14I CCWWGG 2 cut(s) 852, 1131
EcoT38I GRGCYC 2 cut(s) 145, 536
ErhI CCWWGG 2 cut(s) 852, 1131
Esp3I CGTCTC 1 cut(s) 354
FaeI CATG 8 cut(s) 732, 1485, 1755, 1841, 1901, 2102, 2140, 2216
FaqI GGGAC 1 cut(s) 1808
FatI CATG 8 cut(s) 728, 1481, 1751, 1837, 1897, 2098, 2136, 2212
FauI CCCGC 1 cut(s) 2022
FbaI TGATCA 1 cut(s) 1209
FblI GTMKAC 1 cut(s) 2026
FokI GGATG 8 cut(s) 747, 899, 1073, 1180, 1475, 1600, 1683, 1865
FriOI GRGCYC 2 cut(s) 145, 536
FspBI CTAG 6 cut(s) 831, 876, 1503, 1550, 1800, 2331
GlaI GCGC 4 cut(s) 69, 275, 696, 1896
GsaI CCCAGC 4 cut(s) 26, 35, 1350, 1751
GsuI CTGGAG 1 cut(s) 1436
HaeII RGCGCY 1 cut(s) 71
HaeIII GGCC 9 cut(s) 113, 428, 476, 776, 786, 857, 1615, 2175, 2310
HapII CCGG 6 cut(s) 47, 480, 518, 524, 542, 800
HgaI GACGC 4 cut(s) 62, 230, 312, 1843
HhaI GCGC 4 cut(s) 70, 276, 697, 1897
Hin1I GRCGYC 2 cut(s) 222, 323
Hin1II CATG 8 cut(s) 732, 1485, 1755, 1841, 1901, 2102, 2140, 2216
Hin6I GCGC 4 cut(s) 68, 274, 695, 1895
HinP1I GCGC 4 cut(s) 68, 274, 695, 1895
HincII GTYRAC 2 cut(s) 1428, 2027
HindII GTYRAC 2 cut(s) 1428, 2027
HindIII AAGCTT 3 cut(s) 632, 1334, 2219
HpaII CCGG 6 cut(s) 47, 480, 518, 524, 542, 800
HphI GGTGA 4 cut(s) 286, 1078, 1183, 1736
Hpy166II GTNNAC 3 cut(s) 1428, 2027, 2153
Hpy188I TCNGA 9 cut(s) 262, 288, 394, 531, 712, 981, 1002, 1158, 2317
Hpy188III TCNNGA 6 cut(s) 249, 628, 1315, 1540, 1595, 2137
Hpy8I GTNNAC 3 cut(s) 1428, 2027, 2153
Hpy99I CGWCG 8 cut(s) 56, 224, 322, 328, 361, 367, 1036, 2249
HpyCH4III ACNGT 2 cut(s) 100, 1728
HpyCH4IV ACGT 2 cut(s) 281, 2105
HpyF3I CTNAG 9 cut(s) 423, 675, 711, 843, 1044, 1593, 1608, 1956, 2048
HpySE526I ACGT 2 cut(s) 281, 2105
Hsp92I GRCGYC 2 cut(s) 222, 323
Hsp92II CATG 8 cut(s) 732, 1485, 1755, 1841, 1901, 2102, 2140, 2216
HspAI GCGC 4 cut(s) 68, 274, 695, 1895
KroI GCCGGC 1 cut(s) 799
KroNI GCCGGC 1 cut(s) 801
Ksp22I TGATCA 1 cut(s) 1209
Kzo9I GATC 6 cut(s) 468, 1209, 1774, 1942, 1981, 2233
LmnI GCTCC 7 cut(s) 40, 128, 151, 679, 817, 1359, 1912
Lsp1109I GCAGC 4 cut(s) 349, 571, 1105, 1747
LweI GCATC 7 cut(s) 769, 829, 877, 1051, 1317, 2286, 2308
MaeI CTAG 6 cut(s) 831, 876, 1503, 1550, 1800, 2331
MaeII ACGT 2 cut(s) 281, 2105
MaeIII GTNAC 4 cut(s) 94, 366, 1249, 1987
MalI GATC 6 cut(s) 470, 1211, 1776, 1944, 1983, 2235
MbiI CCGCTC 1 cut(s) 1948
MboI GATC 6 cut(s) 468, 1209, 1774, 1942, 1981, 2233
MfeI CAATTG 1 cut(s) 2125
MflI RGATCY 3 cut(s) 1942, 1981, 2233
MhlI GDGCHC 3 cut(s) 145, 267, 536
MlsI TGGCCA 1 cut(s) 786
MluNI TGGCCA 1 cut(s) 786
MlyI GAGTC 3 cut(s) 734, 1162, 1585
MmeI TCCRAC 1 cut(s) 594
Mox20I TGGCCA 1 cut(s) 786
MroNI GCCGGC 1 cut(s) 799
MscI TGGCCA 1 cut(s) 786
MslI CAYNNNNRTG 1 cut(s) 1825
Msp20I TGGCCA 1 cut(s) 786
MspA1I CMGCKG 1 cut(s) 1760
MspI CCGG 6 cut(s) 47, 480, 518, 524, 542, 800
MspR9I CCNGG 9 cut(s) 161, 480, 518, 519, 525, 542, 788, 1885, 2177
MunI CAATTG 1 cut(s) 2125
Mva1269I GAATGC 1 cut(s) 1090
MvaI CCWGG 4 cut(s) 161, 788, 1885, 2177
MvnI CGCG 2 cut(s) 56, 276
NaeI GCCGGC 1 cut(s) 801
NciI CCSGG 5 cut(s) 480, 518, 519, 525, 542
NdeII GATC 6 cut(s) 468, 1209, 1774, 1942, 1981, 2233
NgoMIV GCCGGC 1 cut(s) 799
NlaIII CATG 8 cut(s) 732, 1485, 1755, 1841, 1901, 2102, 2140, 2216
NlaIV GGNNCC 6 cut(s) 153, 429, 477, 522, 528, 1944
NmuCI GTSAC 1 cut(s) 366
NspI RCATGY 2 cut(s) 1485, 1901
NspV TTCGAA 1 cut(s) 103
PaeR7I CTCGAG 1 cut(s) 755
PagI TCATGA 1 cut(s) 2136
PceI AGGCCT 2 cut(s) 776, 2175
PciI ACATGT 1 cut(s) 1481
PcsI WCGNNNNNNNCGW 2 cut(s) 90, 353
PctI GAATGC 1 cut(s) 1090
PdiI GCCGGC 1 cut(s) 801
PfeI GAWTC 6 cut(s) 445, 689, 1159, 1493, 1522, 1536
PflMI CCANNNNNTGG 1 cut(s) 1622
PleI GAGTC 3 cut(s) 733, 1161, 1585
PmaCI CACGTG 1 cut(s) 282
PmlI CACGTG 1 cut(s) 282
PpsI GAGTC 3 cut(s) 733, 1161, 1585
Ppu21I YACGTR 1 cut(s) 282
PpuMI RGGWCCY 1 cut(s) 1700
PscI ACATGT 1 cut(s) 1481
PshAI GACNNNNGTC 1 cut(s) 542
PshBI ATTAAT 2 cut(s) 1619, 1907
Psp5II RGGWCCY 1 cut(s) 1700
Psp6I CCWGG 4 cut(s) 159, 786, 1883, 2175
PspCI CACGTG 1 cut(s) 282
PspFI CCCAGC 4 cut(s) 22, 31, 1346, 1747
PspGI CCWGG 4 cut(s) 159, 786, 1883, 2175
PspN4I GGNNCC 6 cut(s) 153, 429, 477, 522, 528, 1944
PspPI GGNCC 7 cut(s) 163, 427, 475, 521, 527, 1700, 2308
PspPPI RGGWCCY 1 cut(s) 1700
PspXI VCTCGAGB 1 cut(s) 755
PsrI GAACNNNNNNTAC 2 cut(s) 65, 97
PstI CTGCAG 1 cut(s) 1021
PsuI RGATCY 3 cut(s) 1942, 1981, 2233
PvuII CAGCTG 1 cut(s) 1760
RsaI GTAC 6 cut(s) 137, 403, 453, 1322, 1811, 2154
RsaNI GTAC 6 cut(s) 136, 402, 452, 1321, 1810, 2153
RseI CAYNNNNRTG 1 cut(s) 1825
SalI GTCGAC 1 cut(s) 2025
Sau3AI GATC 6 cut(s) 468, 1209, 1774, 1942, 1981, 2233
Sau96I GGNCC 7 cut(s) 163, 427, 475, 521, 527, 1700, 2308
SchI GAGTC 3 cut(s) 734, 1162, 1585
ScrFI CCNGG 9 cut(s) 161, 480, 518, 519, 525, 542, 788, 1885, 2177
SduI GDGCHC 3 cut(s) 145, 267, 536
SfaNI GCATC 7 cut(s) 769, 829, 877, 1051, 1317, 2286, 2308
SfcI CTRYAG 2 cut(s) 1017, 1190
Sfr274I CTCGAG 1 cut(s) 755
SfuI TTCGAA 1 cut(s) 103
SinI GGWCC 4 cut(s) 163, 521, 527, 1700
SlaI CTCGAG 1 cut(s) 755
SmaI CCCGGG 1 cut(s) 519
SmiMI CAYNNNNRTG 1 cut(s) 1825
SmlI CTYRAG 5 cut(s) 203, 269, 698, 755, 2164
SmoI CTYRAG 5 cut(s) 203, 269, 698, 755, 2164
SpeI ACTAGT 1 cut(s) 1799
SseBI AGGCCT 2 cut(s) 776, 2175
SspMI CTAG 6 cut(s) 831, 876, 1503, 1550, 1800, 2331
StuI AGGCCT 2 cut(s) 776, 2175
StyD4I CCNGG 9 cut(s) 159, 478, 516, 517, 523, 540, 786, 1883, 2175
StyI CCWWGG 2 cut(s) 852, 1131
TaaI ACNGT 2 cut(s) 100, 1728
TaiI ACGT 2 cut(s) 284, 2108
TaqI TCGA 7 cut(s) 103, 443, 471, 749, 756, 1034, 2026
TatI WGTACW 3 cut(s) 451, 1320, 2152
TauI GCSGC 6 cut(s) 59, 62, 279, 337, 492, 2210
TfiI GAWTC 6 cut(s) 445, 689, 1159, 1493, 1522, 1536
TscAI CASTG 2 cut(s) 1521, 2085
TseFI GTSAC 1 cut(s) 366
TseI GCWGC 4 cut(s) 337, 559, 1093, 1760
Tsp45I GTSAC 1 cut(s) 366
TspGWI ACGGA 1 cut(s) 289
TspMI CCCGGG 1 cut(s) 517
TspRI CASTG 2 cut(s) 1521, 2085
Van91I CCANNNNNTGG 1 cut(s) 1622
VpaK11BI GGWCC 4 cut(s) 163, 521, 527, 1700
VspI ATTAAT 2 cut(s) 1619, 1907
XapI RAATTY 2 cut(s) 289, 1144
XceI RCATGY 2 cut(s) 1485, 1901
XhoI CTCGAG 1 cut(s) 755
XmaI CCCGGG 1 cut(s) 517
XmiI GTMKAC 1 cut(s) 2026
XspI CTAG 6 cut(s) 831, 876, 1503, 1550, 1800, 2331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.