Prupe.5G080400_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001384
Physical Location & Seq
Reverse (-)
9400278 .. 9405175
4898 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G080400.2

Sequence Viewer

Length: 2331 bp
ATGGCGGCTACCAACACCACCACTCAGGCCGTGGGAGTTCTGGGGGTGGACACGTCAGCGTCGGCCGACGAGGCGGCGGCCAAAGCCGTGCACAAGAGGTACGAAGGGCTTGTGATGGTACGGACGAAGGCGATAAAGGGCAAAGGGGCTTGGTACTGGGCTCACTTGGAGCCTATGCTGGTCCACAACACCGATACTGGGTTGCCCAAGGCGGTGAAGCTCCGGTGCTCCCTATGCGACGCCGTTTTCTCCGCCTCGAACCCGTCGCGCACCGCCTCCGAGCATTTAAAGCGCGGGACTTGCCCCAATTTCAACTCCGTCGCCAAACCCATTTCGTCTCTCTCGCCTTCTTCCACGATCAACTTGCCCCCTTCTCCCACTCCGGTCCACCATAACCATCGGAAGCGAAGCTCGTCTTCGGTTTCCGTTTCGGCCTCGACGTCGTCGTACCATGTTCCCCCGCTTGCGATAGTGGACCCGACTCGGTTCTGCGGCGAGCTCACTTACTCTCCGACGACGGCGACTGCTCAGACGGCGGTAACGGCTGTGACCCATCAGCCGCATTTGGTGTTATCCGGTGGGAAAGACGACTTGGGAGCTCTGGCGATGCTGGAGGACAGTGTGAAGAAGCTCAAGAGTCCCAAAACTTCACCTGGGCCGACGCTGAGTAAGACCCAGGTGGAATTTGCGCTTGATTTTTTGGCTGATTGGGTGTTTGAGTCTTGTGGGTCGGTCTCGTTTTCGAGCCTTGAGCACCCAAAGTTCAGAGCTTTTCTCAACCAGGTGGGTTTACCGTCGATTTCTCGGAGGGAGTTCACTGGGTCGAGACTCGATGCTAAGTTTGAGGAAGCTAAGGCCGAGTCAGAGGCCAGAATTAGAGACGCCATGTTCTTTCAGGTTGCCTCTGATGGGTGGAAGAACAAGAGCTTTGGTGCGTTTGGGGAGGATGGTCTTGTGAATTTGACTGTGAATCTTCCCAATGGGACTAGTTTGTACAGGAGAGCTGTGTTTGTGGGTGGCTCTGTGCCTTCTACGTATGCTGAGGAGGTTTTGTGGGACACAGTGACAAGCATTTGTGGGAATGTTGTGCAGCAGTGTGTAGGAATAGTAGCAGACAAGTTTAAGTCCAAGGCATTGAGGAATTTAGAGACTCAGAATCACTGGATGGTTAATCTTTCTTGTCAGTTTCAGGGGTTCAATAGTTTGATTAAGGATTTCAGTAAGGAACTTCCATTGTTTAAGGCTGTGACTGAAAATTGTTTCAAGCTTGCAAATTTCGTGAATAACAAGTCTCAGGTTAGGAGTAGCTTTCATAAGTATCAGTCCCAAGAATATGGTCATGCTGGGTTATTAAGAGTGCCCTTGCGTGAGTTTGAAATGTTCAACTTTGGATCAGTGCATGTAATGCTTGAGGATATTCTGAGCTCAGCCAGAGCACTTCAGTTGGTCCTGCTGGATGAATCTTATAAAGTGGCGTCCATGGAGGACCCAACAGCTAGGGAAGTTGCTGAGATGATTGGGGATGTGGGGTTTTGGAATGAATTGGAGGCAGTGCATTCCTTGGTTAAGTTGATCAAGGATATGGCTCAAGAAATTGAAACAGAGAGGCCACTGGTTGGGAAATGCCTCCCACTTTGGGATGAGCTAAGAGCAAAAGTGAAGGATTGGTGTTCCAATTTCCACATTGCCGAAGAACCGGTGGAGAAGGTGATTGAAAGGCGGTTCAAGAAGAATTACCATCCAGCTTGGGCTGCAGCATTTATACTTGATCCACTTTATTTGATTAGGGATAATAGTGGGAAGTATCTTCCACCTTTCAAATTGTTGACACCAGAGCAGGAGAAGGATGTGGACAAGCTCATAACCCGGCTTGTGACGAGGGAGGAGGCCCATATTGCACTAATGGAACTCATGAAATGGAGAACAGAAGGGCTTGATCCGGTATATGCACGTGCTGTACAGATGAAGGAAAGGGATCCCATTACAGGCAAGATGAAAATAGCTAACCCACAGAGCAGTCGGCTTGTGTGGGAGACATACCTCACTGAATTTAAGTCACTAGGGAAAGTTGCAGTTAGGCTCATCTTCCTACATGCAACTTCATGTGGGTTCAAATGCAATTGGTCTTTATTGAGATGGGTGTCTGCCCATGGGCACTCAAGGGTGGGCATGGACAAGGCACAGAAGTTGATATTCATTGCAGCTCATTCCAAACTTGAGAGGCGGGATTTTTCCTGTGATGAAGATAAGGATGCAGAGCTACTTGCCTTAGCAAACGGAGCCATTTTTTGCATCCTAAGCAGAAGGTGCTCTGTAATTCAACTGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659

Protein Analysis

777

Amino Acids

86.3

Weight (kDa)

8.66

Isoelectric Point (pI)

37.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1467
AatII GACGTC 1 cut(s) 443
AccB7I CCANNNNNTGG 1 cut(s) 1616
AccII CGCG 2 cut(s) 268, 294
AclWI GGATC 5 cut(s) 1399, 1763, 1931, 1970, 1983
AcoI YGGCCR 2 cut(s) 63, 78
AcsI RAATTY 5 cut(s) 683, 958, 1141, 1273, 2048
AcuI CTGAAG 1 cut(s) 1424
AcvI CACGTG 1 cut(s) 1952
AcyI GRCGYC 4 cut(s) 240, 440, 882, 1475
AfaI GTAC 6 cut(s) 101, 120, 155, 449, 995, 1959
AfiI CCNNNNNNNGG 5 cut(s) 198, 909, 1616, 1636, 1985
AflIII ACRYGT 1 cut(s) 51
AgeI ACCGGT 1 cut(s) 1696
AhlI ACTAGT 1 cut(s) 986
AjiI CACGTC 1 cut(s) 54
AjnI CCWGG 3 cut(s) 652, 675, 780
AjuI GAANNNNNNNTTGG 2 cut(s) 575, 607
Alw21I GWGCWC 8 cut(s) 93, 230, 501, 601, 756, 1427, 1438, 2312
Alw26I GTCTC 7 cut(s) 342, 739, 820, 873, 1142, 1296, 2027
Alw44I GTGCAC 1 cut(s) 89
AlwI GGATC 5 cut(s) 1399, 1763, 1931, 1970, 1983
AoxI GGCC 9 cut(s) 27, 63, 78, 432, 656, 855, 867, 1607, 1887
ApaLI GTGCAC 1 cut(s) 89
ApeKI GCWGC 4 cut(s) 1090, 1751, 1754, 2201
ApoI RAATTY 5 cut(s) 683, 958, 1141, 1273, 2048
AsiGI ACCGGT 1 cut(s) 1696
AspLEI GCGC 3 cut(s) 270, 294, 691
AspS9I GGNCC 7 cut(s) 181, 385, 475, 656, 1447, 1486, 1888
AsuC2I CCSGG 1 cut(s) 1867
AsuHPI GGTGA 3 cut(s) 226, 642, 1720
AvaII GGWCC 5 cut(s) 181, 385, 475, 1447, 1486
BaeGI GKGCMC 3 cut(s) 93, 1362, 2157
BamHI GGATCC 1 cut(s) 1975
BanII GRGCYC 4 cut(s) 163, 501, 601, 1427
BbrPI CACGTG 1 cut(s) 1952
BbsI GAAGAC 1 cut(s) 408
Bbv12I GWGCWC 8 cut(s) 93, 230, 501, 601, 756, 1427, 1438, 2312
BbvCI CCTCAGC 1 cut(s) 1041
BbvI GCAGC 4 cut(s) 1102, 1738, 1766, 2213
BccI CCATC 8 cut(s) 109, 405, 561, 902, 941, 1159, 1746, 2130
BceAI ACGGC 6 cut(s) 14, 71, 227, 534, 549, 558
BcgI CGANNNNNNTGC 2 cut(s) 346, 380
BciT130I CCWGG 3 cut(s) 654, 677, 782
BclI TGATCA 1 cut(s) 1572
BcnI CCSGG 1 cut(s) 1867
BcoDI GTCTC 7 cut(s) 342, 739, 820, 873, 1142, 1296, 2027
BcuI ACTAGT 1 cut(s) 986
BfaI CTAG 3 cut(s) 987, 1497, 2060
BfmI CTRYAG 1 cut(s) 1752
BglI GCCNNNNNGGC 1 cut(s) 71
BisI GCNGC 9 cut(s) 6, 75, 78, 493, 560, 1091, 1752, 1755, 2202
BlpI GCTNAGC 1 cut(s) 1426
BlsI GCNGC 9 cut(s) 7, 76, 79, 494, 561, 1092, 1753, 1756, 2203
Bme1390I CCNGG 4 cut(s) 654, 677, 782, 1867
Bme18I GGWCC 5 cut(s) 181, 385, 475, 1447, 1486
BmgBI CACGTC 1 cut(s) 54
BmgT120I GGNCC 7 cut(s) 181, 385, 475, 656, 1447, 1486, 1888
BmiI GGNNCC 5 cut(s) 171, 477, 1488, 1977, 2281
BmrFI CCNGG 4 cut(s) 654, 677, 782, 1867
BmrI ACTGGG 3 cut(s) 166, 207, 828
BmsI GCATC 4 cut(s) 597, 823, 2242, 2301
BmuI ACTGGG 3 cut(s) 166, 207, 828
BpiI GAAGAC 1 cut(s) 408
BplI GAGNNNNNCTC 2 cut(s) 759, 791
BpmI CTGGAG 1 cut(s) 632
Bpu10I CCTNAGC 4 cut(s) 852, 1041, 2269, 2297
Bpu1102I GCTNAGC 1 cut(s) 1426
BpuEI CTTGAG 6 cut(s) 617, 770, 1430, 1572, 2143, 2237
BpuMI CCSGG 1 cut(s) 1867
BsaAI YACGTR 2 cut(s) 1035, 1952
BsaHI GRCGYC 4 cut(s) 240, 440, 882, 1475
BsaI GGTCTC 1 cut(s) 739
BsaJI CCNNGG 8 cut(s) 30, 207, 653, 675, 1128, 1479, 1560, 2149
BsaWI WCCGGW 5 cut(s) 222, 382, 575, 1696, 1939
BsaXI ACNNNNNCTCC 4 cut(s) 493, 523, 1037, 1067
Bsc4I CCNNNNNNNGG 5 cut(s) 198, 909, 1616, 1636, 1985
Bse118I RCCGGY 1 cut(s) 1696
Bse1I ACTGG 5 cut(s) 161, 202, 823, 1166, 1617
Bse3DI GCAATG 2 cut(s) 1683, 2196
BseBI CCWGG 3 cut(s) 654, 677, 782
BseDI CCNNGG 8 cut(s) 30, 207, 653, 675, 1128, 1479, 1560, 2149
BseGI GGATG 9 cut(s) 952, 1170, 1462, 1528, 1645, 1738, 1852, 2257, 2292
BseLI CCNNNNNNNGG 5 cut(s) 198, 909, 1616, 1636, 1985
BseMI GCAATG 2 cut(s) 1683, 2196
BseMII CTCAG 9 cut(s) 38, 542, 656, 1032, 1166, 1307, 1412, 1440, 1500
BseNI ACTGG 5 cut(s) 161, 202, 823, 1166, 1617
BseRI GAGGAG 2 cut(s) 1058, 1898
BseSI GKGCMC 3 cut(s) 93, 1362, 2157
BseX3I CGGCCG 1 cut(s) 63
BseXI GCAGC 4 cut(s) 1102, 1738, 1766, 2213
BseYI CCCAGC 1 cut(s) 1343
BsgI GTGCAG 1 cut(s) 1109
Bsh1236I CGCG 2 cut(s) 268, 294
Bsh1285I CGRYCG 1 cut(s) 66
BshFI GGCC 9 cut(s) 29, 65, 80, 434, 658, 857, 869, 1609, 1889
BshTI ACCGGT 1 cut(s) 1696
BsiEI CGRYCG 1 cut(s) 66
BsiHKAI GWGCWC 8 cut(s) 93, 230, 501, 601, 756, 1427, 1438, 2312
BsiSI CCGG 6 cut(s) 223, 383, 576, 1697, 1867, 1940
BslFI GGGAC 5 cut(s) 310, 624, 997, 1070, 1309
BslI CCNNNNNNNGG 5 cut(s) 198, 909, 1616, 1636, 1985
BsmAI GTCTC 7 cut(s) 342, 739, 820, 873, 1142, 1296, 2027
BsmBI CGTCTC 2 cut(s) 342, 873
BsmFI GGGAC 5 cut(s) 310, 624, 997, 1070, 1309
BsmI GAATGC 1 cut(s) 1555
BsnI GGCC 9 cut(s) 29, 65, 80, 434, 658, 857, 869, 1609, 1889
Bso31I GGTCTC 1 cut(s) 739
Bsp1407I TGTACA 2 cut(s) 993, 1957
Bsp143I GATC 6 cut(s) 357, 1391, 1572, 1768, 1936, 1975
Bsp1720I GCTNAGC 1 cut(s) 1426
Bsp19I CCATGG 2 cut(s) 1479, 2149
BspANI GGCC 9 cut(s) 29, 65, 80, 434, 658, 857, 869, 1609, 1889
BspCNI CTCAG 9 cut(s) 37, 541, 657, 1033, 1165, 1306, 1413, 1439, 1501
BspFNI CGCG 2 cut(s) 268, 294
BspHI TCATGA 1 cut(s) 1911
BspLI GGNNCC 5 cut(s) 171, 477, 1488, 1977, 2281
BspMAI CTGCAG 1 cut(s) 1756
BspPI GGATC 5 cut(s) 1399, 1763, 1931, 1970, 1983
BspTNI GGTCTC 1 cut(s) 739
BsrDI GCAATG 2 cut(s) 1683, 2196
BsrFI RCCGGY 1 cut(s) 1696
BsrGI TGTACA 2 cut(s) 993, 1957
BsrI ACTGG 5 cut(s) 161, 202, 823, 1166, 1617
BssAI RCCGGY 1 cut(s) 1696
BssECI CCNNGG 8 cut(s) 30, 207, 653, 675, 1128, 1479, 1560, 2149
BssMI GATC 6 cut(s) 357, 1391, 1572, 1768, 1936, 1975
BssNI GRCGYC 4 cut(s) 240, 440, 882, 1475
BssT1I CCWWGG 5 cut(s) 207, 1128, 1479, 1560, 2149
Bst2UI CCWGG 3 cut(s) 654, 677, 782
Bst4CI ACNGT 5 cut(s) 620, 795, 967, 1063, 2325
BstACI GRCGYC 4 cut(s) 240, 440, 882, 1475
BstAPI GCANNNNNTGC 2 cut(s) 1405, 2307
BstAUI TGTACA 2 cut(s) 993, 1957
BstBAI YACGTR 2 cut(s) 1035, 1952
BstC8I GCNNGC 3 cut(s) 465, 497, 1269
BstDSI CCRYGG 3 cut(s) 30, 1479, 2149
BstF5I GGATG 9 cut(s) 952, 1170, 1462, 1528, 1645, 1738, 1852, 2257, 2292
BstFNI CGCG 2 cut(s) 268, 294
BstHHI GCGC 3 cut(s) 270, 294, 691
BstKTI GATC 6 cut(s) 360, 1394, 1575, 1771, 1939, 1978
BstMAI GTCTC 7 cut(s) 342, 739, 820, 873, 1142, 1296, 2027
BstMBI GATC 6 cut(s) 357, 1391, 1572, 1768, 1936, 1975
BstMCI CGRYCG 1 cut(s) 66
BstNI CCWGG 3 cut(s) 654, 677, 782
BstNSI RCATGY 2 cut(s) 1403, 2096
BstSCI CCNGG 4 cut(s) 652, 675, 780, 1865
BstSFI CTRYAG 1 cut(s) 1752
BstSLI GKGCMC 3 cut(s) 93, 1362, 2157
BstSNI TACGTA 1 cut(s) 1035
BstUI CGCG 2 cut(s) 268, 294
BstV1I GCAGC 4 cut(s) 1102, 1738, 1766, 2213
BstV2I GAAGAC 1 cut(s) 408
BstX2I RGATCY 1 cut(s) 1975
BstXI CCANNNNNNTGG 1 cut(s) 1334
BstYI RGATCY 1 cut(s) 1975
BstZI CGGCCG 1 cut(s) 63
BsuRI GGCC 9 cut(s) 29, 65, 80, 434, 658, 857, 869, 1609, 1889
BtgI CCRYGG 3 cut(s) 30, 1479, 2149
BtgZI GCGATG 1 cut(s) 620
BtrI CACGTC 1 cut(s) 54
BtsCI GGATG 9 cut(s) 952, 1170, 1462, 1528, 1645, 1738, 1852, 2257, 2292
BtsI GCAGTG 2 cut(s) 1100, 1557
BtsIMutI CAGTG 9 cut(s) 625, 816, 1068, 1100, 1159, 1401, 1557, 1610, 2043
Cac8I GCNNGC 3 cut(s) 465, 497, 1269
CciI TCATGA 1 cut(s) 1911
CfoI GCGC 3 cut(s) 270, 294, 691
Cfr10I RCCGGY 1 cut(s) 1696
Cfr13I GGNCC 7 cut(s) 181, 385, 475, 656, 1447, 1486, 1888
CseI GACGC 5 cut(s) 48, 248, 670, 890, 1464
CsiI ACCWGGT 1 cut(s) 780
Csp6I GTAC 6 cut(s) 100, 119, 154, 448, 994, 1958
CspAI ACCGGT 1 cut(s) 1696
CspCI CAANNNNNGTGG 2 cut(s) 1761, 1796
CviQI GTAC 6 cut(s) 100, 119, 154, 448, 994, 1958
DpnI GATC 6 cut(s) 359, 1393, 1574, 1770, 1938, 1977
DpnII GATC 6 cut(s) 357, 1391, 1572, 1768, 1936, 1975
DraI TTTAAA 1 cut(s) 288
EaeI YGGCCR 2 cut(s) 63, 78
EagI CGGCCG 1 cut(s) 63
EciI GGCGGA 1 cut(s) 241
Ecl136II GAGCTC 3 cut(s) 499, 599, 1425
EclXI CGGCCG 1 cut(s) 63
Eco105I TACGTA 1 cut(s) 1035
Eco130I CCWWGG 5 cut(s) 207, 1128, 1479, 1560, 2149
Eco24I GRGCYC 4 cut(s) 163, 501, 601, 1427
Eco31I GGTCTC 1 cut(s) 739
Eco47I GGWCC 5 cut(s) 181, 385, 475, 1447, 1486
Eco52I CGGCCG 1 cut(s) 63
Eco53kI GAGCTC 3 cut(s) 499, 599, 1425
Eco57I CTGAAG 1 cut(s) 1424
Eco72I CACGTG 1 cut(s) 1952
EcoICRI GAGCTC 3 cut(s) 499, 599, 1425
EcoO109I RGGNCCY 1 cut(s) 1486
EcoRII CCWGG 3 cut(s) 652, 675, 780
EcoT14I CCWWGG 5 cut(s) 207, 1128, 1479, 1560, 2149
EcoT38I GRGCYC 4 cut(s) 163, 501, 601, 1427
ErhI CCWWGG 5 cut(s) 207, 1128, 1479, 1560, 2149
Esp3I CGTCTC 2 cut(s) 342, 873
FalI AAGNNNNNCTT 2 cut(s) 400, 432
FaqI GGGAC 5 cut(s) 310, 624, 997, 1070, 1309
FauI CCCGC 3 cut(s) 287, 468, 2217
FbaI TGATCA 1 cut(s) 1572
Fnu4HI GCNGC 9 cut(s) 6, 75, 78, 493, 560, 1091, 1752, 1755, 2202
FokI GGATG 9 cut(s) 959, 1177, 1469, 1535, 1652, 1725, 1859, 2264, 2279
FriOI GRGCYC 4 cut(s) 163, 501, 601, 1427
Fsp4HI GCNGC 9 cut(s) 6, 75, 78, 493, 560, 1091, 1752, 1755, 2202
FspBI CTAG 3 cut(s) 987, 1497, 2060
GlaI GCGC 3 cut(s) 269, 293, 690
GluI GCNGC 9 cut(s) 6, 75, 78, 493, 560, 1091, 1752, 1755, 2202
GsaI CCCAGC 1 cut(s) 1347
GsuI CTGGAG 1 cut(s) 632
HaeIII GGCC 9 cut(s) 29, 65, 80, 434, 658, 857, 869, 1609, 1889
HapII CCGG 6 cut(s) 223, 383, 576, 1697, 1867, 1940
HgaI GACGC 5 cut(s) 48, 248, 670, 890, 1464
HhaI GCGC 3 cut(s) 270, 294, 691
Hin1I GRCGYC 4 cut(s) 240, 440, 882, 1475
Hin6I GCGC 3 cut(s) 268, 292, 689
HinP1I GCGC 3 cut(s) 268, 292, 689
HincII GTYRAC 1 cut(s) 1827
HindII GTYRAC 1 cut(s) 1827
HindIII AAGCTT 1 cut(s) 1265
HinfI GANTC 9 cut(s) 481, 637, 719, 828, 860, 970, 1150, 1156, 1460
HpaII CCGG 6 cut(s) 223, 383, 576, 1697, 1867, 1940
HphI GGTGA 3 cut(s) 226, 642, 1720
Hpy166II GTNNAC 9 cut(s) 49, 91, 184, 388, 475, 791, 816, 1827, 1852
Hpy188III TCNNGA 6 cut(s) 634, 825, 1279, 1589, 1726, 1912
Hpy8I GTNNAC 9 cut(s) 49, 91, 184, 388, 475, 791, 816, 1827, 1852
HpyCH4III ACNGT 5 cut(s) 620, 795, 967, 1063, 2325
HpyCH4IV ACGT 4 cut(s) 53, 440, 1034, 1951
HpySE526I ACGT 4 cut(s) 53, 440, 1034, 1951
Hsp92I GRCGYC 4 cut(s) 240, 440, 882, 1475
HspAI GCGC 3 cut(s) 268, 292, 689
Ksp22I TGATCA 1 cut(s) 1572
Kzo9I GATC 6 cut(s) 357, 1391, 1572, 1768, 1936, 1975
LmnI GCTCC 5 cut(s) 169, 225, 233, 596, 2279
Lsp1109I GCAGC 4 cut(s) 1102, 1738, 1766, 2213
LweI GCATC 4 cut(s) 597, 823, 2242, 2301
MabI ACCWGGT 1 cut(s) 780
MaeI CTAG 3 cut(s) 987, 1497, 2060
MaeII ACGT 4 cut(s) 53, 440, 1034, 1951
MaeIII GTNAC 6 cut(s) 538, 547, 1063, 1246, 1873, 2055
MalI GATC 6 cut(s) 359, 1393, 1574, 1770, 1938, 1977
MboI GATC 6 cut(s) 357, 1391, 1572, 1768, 1936, 1975
MfeI CAATTG 1 cut(s) 2119
MflI RGATCY 1 cut(s) 1975
MlyI GAGTC 6 cut(s) 475, 646, 728, 822, 869, 1144
MmeI TCCRAC 1 cut(s) 536
MseI TTAA 8 cut(s) 287, 1122, 1170, 1209, 1239, 1352, 1566, 2052
MspI CCGG 6 cut(s) 223, 383, 576, 1697, 1867, 1940
MspR9I CCNGG 4 cut(s) 654, 677, 782, 1867
MunI CAATTG 1 cut(s) 2119
Mva1269I GAATGC 1 cut(s) 1555
MvaI CCWGG 3 cut(s) 654, 677, 782
MvnI CGCG 2 cut(s) 268, 294
NciI CCSGG 1 cut(s) 1867
NcoI CCATGG 2 cut(s) 1479, 2149
NdeII GATC 6 cut(s) 357, 1391, 1572, 1768, 1936, 1975
NlaIV GGNNCC 5 cut(s) 171, 477, 1488, 1977, 2281
NmeAIII GCCGAG 1 cut(s) 883
NmuCI GTSAC 5 cut(s) 547, 1063, 1246, 1873, 2055
NspI RCATGY 2 cut(s) 1403, 2096
PagI TCATGA 1 cut(s) 1911
PcsI WCGNNNNNNNCGW 2 cut(s) 437, 443
PctI GAATGC 1 cut(s) 1555
PfeI GAWTC 3 cut(s) 970, 1156, 1460
PflFI GACNNNGTC 1 cut(s) 442
PflMI CCANNNNNTGG 1 cut(s) 1616
PinAI ACCGGT 1 cut(s) 1696
PkrI GCNGC 9 cut(s) 7, 76, 79, 494, 561, 1092, 1753, 1756, 2203
PleI GAGTC 6 cut(s) 475, 645, 727, 822, 868, 1144
PmaCI CACGTG 1 cut(s) 1952
PmlI CACGTG 1 cut(s) 1952
PpsI GAGTC 6 cut(s) 475, 645, 727, 822, 868, 1144
Ppu21I YACGTR 2 cut(s) 1035, 1952
PpuMI RGGWCCY 1 cut(s) 1486
PsiI TTATAA 1 cut(s) 1467
Psp124BI GAGCTC 3 cut(s) 501, 601, 1427
Psp5II RGGWCCY 1 cut(s) 1486
Psp6I CCWGG 3 cut(s) 652, 675, 780
PspCI CACGTG 1 cut(s) 1952
PspFI CCCAGC 1 cut(s) 1343
PspGI CCWGG 3 cut(s) 652, 675, 780
PspN4I GGNNCC 5 cut(s) 171, 477, 1488, 1977, 2281
PspPI GGNCC 7 cut(s) 181, 385, 475, 656, 1447, 1486, 1888
PspPPI RGGWCCY 1 cut(s) 1486
PstI CTGCAG 1 cut(s) 1756
PsuI RGATCY 1 cut(s) 1975
PsyI GACNNNGTC 1 cut(s) 442
RsaI GTAC 6 cut(s) 101, 120, 155, 449, 995, 1959
RsaNI GTAC 6 cut(s) 100, 119, 154, 448, 994, 1958
SacI GAGCTC 3 cut(s) 501, 601, 1427
SaqAI TTAA 8 cut(s) 287, 1122, 1170, 1209, 1239, 1352, 1566, 2052
SatI GCNGC 9 cut(s) 6, 75, 78, 493, 560, 1091, 1752, 1755, 2202
Sau3AI GATC 6 cut(s) 357, 1391, 1572, 1768, 1936, 1975
Sau96I GGNCC 7 cut(s) 181, 385, 475, 656, 1447, 1486, 1888
SchI GAGTC 6 cut(s) 475, 646, 728, 822, 869, 1144
ScrFI CCNGG 4 cut(s) 654, 677, 782, 1867
SexAI ACCWGGT 1 cut(s) 780
SfaNI GCATC 4 cut(s) 597, 823, 2242, 2301
SfcI CTRYAG 1 cut(s) 1752
SinI GGWCC 5 cut(s) 181, 385, 475, 1447, 1486
SmlI CTYRAG 6 cut(s) 632, 749, 1409, 1587, 2158, 2216
SmoI CTYRAG 6 cut(s) 632, 749, 1409, 1587, 2158, 2216
SnaBI TACGTA 1 cut(s) 1035
SpeI ACTAGT 1 cut(s) 986
SspMI CTAG 3 cut(s) 987, 1497, 2060
SstI GAGCTC 3 cut(s) 501, 601, 1427
StyD4I CCNGG 4 cut(s) 652, 675, 780, 1865
StyI CCWWGG 5 cut(s) 207, 1128, 1479, 1560, 2149
TaaI ACNGT 5 cut(s) 620, 795, 967, 1063, 2325
TaiI ACGT 4 cut(s) 56, 443, 1037, 1954
TaqI TCGA 6 cut(s) 257, 437, 743, 797, 824, 831
TaqII GACCGA 1 cut(s) 721
TatI WGTACW 2 cut(s) 993, 1957
TauI GCSGC 5 cut(s) 8, 77, 80, 495, 562
TfiI GAWTC 3 cut(s) 970, 1156, 1460
Tru1I TTAA 8 cut(s) 287, 1122, 1170, 1209, 1239, 1352, 1566, 2052
Tru9I TTAA 8 cut(s) 287, 1122, 1170, 1209, 1239, 1352, 1566, 2052
TscAI CASTG 9 cut(s) 625, 823, 1068, 1100, 1166, 1401, 1557, 1617, 2050
TseFI GTSAC 5 cut(s) 547, 1063, 1246, 1873, 2055
TseI GCWGC 4 cut(s) 1090, 1751, 1754, 2201
Tsp45I GTSAC 5 cut(s) 547, 1063, 1246, 1873, 2055
TspDTI ATGAA 9 cut(s) 1301, 1473, 1554, 1928, 1979, 2009, 2091, 2185, 2256
TspGWI ACGGA 4 cut(s) 136, 307, 415, 2292
TspRI CASTG 9 cut(s) 625, 823, 1068, 1100, 1166, 1401, 1557, 1617, 2050
Tth111I GACNNNGTC 1 cut(s) 442
Van91I CCANNNNNTGG 1 cut(s) 1616
VneI GTGCAC 1 cut(s) 89
VpaK11BI GGWCC 5 cut(s) 181, 385, 475, 1447, 1486
XapI RAATTY 5 cut(s) 683, 958, 1141, 1273, 2048
XceI RCATGY 2 cut(s) 1403, 2096
XcmI CCANNNNNNNNNTGG 1 cut(s) 28
XspI CTAG 3 cut(s) 987, 1497, 2060
ZraI GACGTC 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.