pycom07g23050

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001384
Physical Location & Seq
Reverse (-)
24129405 .. 24133565
4161 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g23050.2

Sequence Viewer

Length: 2448 bp
ATGGCGTCCACAAACTCAACCCCAACAGAACCATCTCTCTCGCCGGACGACATAGCCGCCAGGGCCCTCAACCGGCGATTCGAAAGCCTGGTAACTGTTCGAACAAAGGCCATAAAGGGGAAAGGAGCTTGGTACTGGGCTCACTTGGAGCCCGTTCTCGTTTGCGCCCCCAACTCCAACATCCCCAAGGCCGTCAAGCTCAAGTGCTCACTCTGCGATGCCGTCTTCTCCGCCTCCAACCCCTCCAGAACCGCCTCAGAGCATCTCAAACGGGGTACCTGCCCGAATTTCACCTCTGTTTTGAGACCCAATTCATCGGCGGCGGTATCTCCGGTTCCGATATCTTCCTTGCCTTCTCCGTCGTCTCACAATCACAGAAAGCGGAGCTCTCAAATGGGTACTAGTCCTTCTCCTATTTCTCAAGCTCCTCCGCACATTAGTCCTAGTCCGATACAGGTTCACTCTTTAGCTATGATCGAGTCTTCCCGCTTCGGCGGTGACCATAATTTCTCACAGTCTCCAAACCCTGTTGGGATTGCGAGAAGTACGGGGCTGGATCAGCACCATTTGGTTTTATCTGGTGGGAAGGAGGATTTGGGTGCTTTGGCAATGTTAGAAAATAGTGTGAAGAAACTTAAGAGTCCCAAAGCTTCGCCTGGTGCCACTCTTAGTAAGGAGCAGATTGATTCTGCACTCCAACTACTGTCTGAGTGGTTCTATGAGTCATGTGGGTCCGTCTCATTTTCGAGCCTCGAGCATCCCAAGTTCAGGGCTTTCCTTAGCCAGGTGGGCTTGCCTGCATTGCTGCAGCGTGAGCTTTCTGGTGCTCGACTTGATGCTAAGTTTGATGAGGTGAAAGCTGAGTCTGAAGCTAGGATTAGAGATGCAATGTTTTTCCAAGTTGCTTCTGATGGGTGGAAAAGCGGGGATTCTTGTGGGGAAGAGAATGTTGTTAAGTTCACAGTTAATCTTCCGAATGGGATTAGTGTTTTCCAGAAGGTGGTGTTTACTGGCGGGTCAGTTTCTTCAAAATATGCAGAGGAGGTGTTGTGGGATTCAGTTACTGGAGTATGTGGGAATGCTGTGCAGCGTTGTGTGGGGATCGTTGCAGACAAATATAAGGCCAAGGCATTGAGGAACTTGGAGACTCAGAACCATTGGATGGTGAATGTCTCCTGTCAGCTTCAGGGGTTCATATCTTTGATTAAGGATTTTAACAAAGAGCTTCCACTGTTTAGGGTTGTCATTGAAAATTGCTTAAAGGTTGCGAATTTTGTAAATAGCAACTCTGAGGTTAGGCATGCTTTCGAGAAGTGCAAGATACAAGAGCTTGAGTATGCTGGGTTGCTCCAAGTTCCTTCGCCCAAATGTGATACTTCAAAGAACTTTGCACCTATCTACGCAATGCTGGAGGATATATTGAGTTGTGCGCGCATACTCCAAATGGTCGTCTTGGTTGATTTTTATAAGGCGATATGTCTGGAGGATCCAATTGCTAGGGAAGTTGCAGGGATGATTCAAACTGAAGGATTTTGGAATGAATTAGAGGCAGTTTATTCACTTGTTAAGATGATCAGAGGGATGGCTCAGGAGATTGAGGCTGAGAGGCCATTAATTGGGCAATGTCTACCACTTTGGGAAGAATTGAGAACAAAAGTAAAGGATTGGTGTGCCAAGTTCAACATTGTTGAAGGACCTGTTGAGGAAATAGTTGAAAAACGATTCAGAAAGAACTACCACCCAGCATGGTCGGCTGCATTTATCCTTGACCCGATATACTTGATGAGGGACACTAGTGGAAAGTACCTTCCACCATTCAAGTGCTTGACACACGAGCAGGAGAAGGATGTAGATAAGCTCATTACCAGGTTGGTTTCCAGAGAAGAAGCTCATGTTGCATTGATGGAGCTCATGAAATGGAGAACAGAAGGAATGGACCCACTTTATGCTCAGGCAGTGCAGGTTAAACAGCGAGATCCTGTAACTGGAAAGATGAAAATGGCAAACCCGCAGAGCAGCAGACTTGTGTGGGAAACTTGTCTAAGTGAGTTAAAGACATTGGGGAGGGTTGCAGTGAGACTGATCTTCCTCCATGCAACCTCATGTGGATTTAAGTGCAATTGGTCTTTCATGAGATGGATTCGTGTACATAGGCACTCAAGGGTAGGCCTGGAAAGGGCACAGAAGATGATATTTATTGCAGCTCATGCCAAACTTGAGAGAAGGGATCTTTCTAGTGAGGAAGAAAAGGAAGCAGAGCTGTTTGGGACTGCAGACGTTGAGGATGACATGCTGACTGAGGTCTTTTCCGATGCACCCACAGTGATAGGGTTTTCCCGAATGGGTTGGGTGGATGGCAAAACCAGGCTTGAGTCATCACACGGGTCAAACCTCGTAAGAACACAAGAGCGAATCTTTGTAGCCAAAGACACACTTCTCACTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

816

Amino Acids

90.83

Weight (kDa)

8.25

Isoelectric Point (pI)

50.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1467
Acc36I ACCTGC 2 cut(s) 287, 1951
Acc65I GGTACC 1 cut(s) 275
AccB1I GGYRCC 2 cut(s) 275, 659
AccB7I CCANNNNNTGG 3 cut(s) 1000, 1162, 1616
AccI GTMKAC 1 cut(s) 1627
AccII CGCG 1 cut(s) 1432
AclWI GGATC 6 cut(s) 564, 1109, 1481, 1494, 1970, 2235
AcsI RAATTY 2 cut(s) 286, 1270
AcuI CTGAAG 3 cut(s) 888, 1169, 1545
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 6 cut(s) 134, 277, 400, 547, 1805, 2148
AfiI CCNNNNNNNGG 9 cut(s) 72, 117, 768, 784, 1000, 1162, 1616, 1985, 2176
AflII CTTAAG 1 cut(s) 635
AgsI TTSAA 8 cut(s) 1029, 1250, 1380, 1520, 1681, 1691, 1715, 1819
AhlI ACTAGT 2 cut(s) 401, 1793
AjnI CCWGG 7 cut(s) 59, 87, 655, 783, 1865, 2169, 2363
AjuI GAANNNNNNNTTGG 2 cut(s) 578, 610
Alw21I GWGCWC 4 cut(s) 209, 389, 829, 1911
Alw26I GTCTC 7 cut(s) 298, 369, 522, 742, 1139, 1177, 2071
AlwI GGATC 6 cut(s) 564, 1109, 1481, 1494, 1970, 2235
AlwNI CAGNNNCTG 1 cut(s) 1064
Ama87I CYCGRG 1 cut(s) 752
AoxI GGCC 6 cut(s) 63, 108, 189, 1122, 1607, 2167
ApaI GGGCCC 1 cut(s) 67
ApeKI GCWGC 6 cut(s) 805, 808, 1087, 1754, 2016, 2201
ApoI RAATTY 2 cut(s) 286, 1270
AseI ATTAAT 1 cut(s) 1613
Asp718I GGTACC 1 cut(s) 275
AspLEI GCGC 3 cut(s) 167, 1432, 1434
AspS9I GGNCC 5 cut(s) 63, 64, 732, 1694, 1936
AsuHPI GGTGA 4 cut(s) 283, 509, 865, 1177
AsuII TTCGAA 2 cut(s) 81, 100
AvaI CYCGRG 1 cut(s) 752
AvaII GGWCC 3 cut(s) 732, 1694, 1936
BaeGI GKGCMC 2 cut(s) 67, 2182
BamHI GGATCC 1 cut(s) 1486
BanI GGYRCC 2 cut(s) 275, 659
BanII GRGCYC 5 cut(s) 67, 142, 153, 389, 1911
BarI GAAGNNNNNNTAC 2 cut(s) 391, 423
BauI CACGAG 1 cut(s) 1832
BbsI GAAGAC 2 cut(s) 217, 474
Bbv12I GWGCWC 4 cut(s) 209, 389, 829, 1911
BbvI GCAGC 6 cut(s) 792, 820, 1099, 1741, 2028, 2213
BccI CCATC 7 cut(s) 40, 905, 1156, 1576, 1897, 2130, 2348
BceAI ACGGC 2 cut(s) 176, 206
BciT130I CCWGG 7 cut(s) 61, 89, 657, 785, 1867, 2171, 2365
BclI TGATCA 1 cut(s) 1572
BcoDI GTCTC 7 cut(s) 298, 369, 522, 742, 1139, 1177, 2071
BcuI ACTAGT 2 cut(s) 401, 1793
BfaI CTAG 6 cut(s) 402, 444, 873, 1497, 1794, 2235
BfmI CTRYAG 2 cut(s) 806, 2271
BfrI CTTAAG 1 cut(s) 635
BfuAI ACCTGC 2 cut(s) 287, 1951
BglI GCCNNNNNGGC 2 cut(s) 62, 789
BisI GCNGC 8 cut(s) 57, 321, 806, 809, 1088, 1755, 2017, 2202
BlsI GCNGC 8 cut(s) 58, 322, 807, 810, 1089, 1756, 2018, 2203
Bme1390I CCNGG 7 cut(s) 61, 89, 657, 785, 1867, 2171, 2365
Bme18I GGWCC 3 cut(s) 732, 1694, 1936
BmeT110I CYCGRG 1 cut(s) 752
BmgT120I GGNCC 5 cut(s) 63, 64, 732, 1694, 1936
BmiI GGNNCC 8 cut(s) 65, 150, 277, 336, 661, 733, 1488, 1938
BmrFI CCNGG 7 cut(s) 61, 89, 657, 785, 1867, 2171, 2365
BmrI ACTGGG 1 cut(s) 145
BmsI GCATC 6 cut(s) 208, 271, 766, 826, 874, 2302
BmuI ACTGGG 1 cut(s) 145
BoxI GACNNNNGTC 1 cut(s) 2300
BpiI GAAGAC 2 cut(s) 217, 474
BpmI CTGGAG 4 cut(s) 229, 1086, 1430, 1502
Bpu10I CCTNAGC 3 cut(s) 779, 1587, 1950
Bpu14I TTCGAA 2 cut(s) 81, 100
BpuEI CTTGAG 6 cut(s) 185, 405, 1352, 2143, 2237, 2390
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 298
BsaJI CCNNGG 3 cut(s) 60, 186, 1125
BsaWI WCCGGW 1 cut(s) 331
BsaXI ACNNNNNCTCC 2 cut(s) 1034, 1064
Bsc4I CCNNNNNNNGG 9 cut(s) 72, 117, 768, 784, 1000, 1162, 1616, 1985, 2176
Bse118I RCCGGY 1 cut(s) 72
Bse1I ACTGG 4 cut(s) 140, 1015, 1069, 1990
Bse3DI GCAATG 5 cut(s) 615, 800, 894, 1410, 1628
BseBI CCWGG 7 cut(s) 61, 89, 657, 785, 1867, 2171, 2365
BseDI CCNNGG 3 cut(s) 60, 186, 1125
BseGI GGATG 8 cut(s) 180, 757, 1167, 1518, 1587, 1852, 2290, 2359
BseLI CCNNNNNNNGG 9 cut(s) 72, 117, 768, 784, 1000, 1162, 1616, 1985, 2176
BseMI GCAATG 5 cut(s) 615, 800, 894, 1410, 1628
BseMII CTCAG 9 cut(s) 270, 699, 852, 1163, 1281, 1593, 1601, 1964, 2289
BseNI ACTGG 4 cut(s) 140, 1015, 1069, 1990
BsePI GCGCGC 1 cut(s) 1430
BseRI GAGGAG 2 cut(s) 417, 1055
BseSI GKGCMC 2 cut(s) 67, 2182
BseXI GCAGC 6 cut(s) 792, 820, 1099, 1741, 2028, 2213
BseYI CCCAGC 2 cut(s) 1340, 1741
BsgI GTGCAG 3 cut(s) 675, 1106, 1979
Bsh1236I CGCG 1 cut(s) 1432
BshFI GGCC 6 cut(s) 65, 110, 191, 1124, 1609, 2169
BshNI GGYRCC 2 cut(s) 275, 659
BsiHKAI GWGCWC 4 cut(s) 209, 389, 829, 1911
BsiHKCI CYCGRG 1 cut(s) 752
BsiSI CCGG 3 cut(s) 44, 73, 332
BslFI GGGAC 3 cut(s) 627, 1802, 2281
BslI CCNNNNNNNGG 9 cut(s) 72, 117, 768, 784, 1000, 1162, 1616, 1985, 2176
BsmAI GTCTC 7 cut(s) 298, 369, 522, 742, 1139, 1177, 2071
BsmBI CGTCTC 2 cut(s) 369, 742
BsmFI GGGAC 3 cut(s) 627, 1802, 2281
BsmI GAATGC 1 cut(s) 1084
BsnI GGCC 6 cut(s) 65, 110, 191, 1124, 1609, 2169
Bso31I GGTCTC 1 cut(s) 298
BsoBI CYCGRG 1 cut(s) 752
Bsp119I TTCGAA 2 cut(s) 81, 100
Bsp120I GGGCCC 1 cut(s) 63
Bsp1286I GDGCHC 8 cut(s) 67, 142, 153, 209, 389, 829, 1911, 2182
Bsp1407I TGTACA 1 cut(s) 2146
Bsp143I GATC 8 cut(s) 474, 556, 1101, 1486, 1572, 1975, 2082, 2227
BspANI GGCC 6 cut(s) 65, 110, 191, 1124, 1609, 2169
BspCNI CTCAG 9 cut(s) 269, 700, 853, 1162, 1282, 1594, 1600, 1963, 2290
BspFNI CGCG 1 cut(s) 1432
BspHI TCATGA 2 cut(s) 1911, 2130
BspLI GGNNCC 8 cut(s) 65, 150, 277, 336, 661, 733, 1488, 1938
BspMAI CTGCAG 2 cut(s) 810, 2275
BspMI ACCTGC 2 cut(s) 287, 1951
BspPI GGATC 6 cut(s) 564, 1109, 1481, 1494, 1970, 2235
BspT104I TTCGAA 2 cut(s) 81, 100
BspT107I GGYRCC 2 cut(s) 275, 659
BspTI CTTAAG 1 cut(s) 635
BspTNI GGTCTC 1 cut(s) 298
BsrDI GCAATG 5 cut(s) 615, 800, 894, 1410, 1628
BsrFI RCCGGY 1 cut(s) 72
BsrGI TGTACA 1 cut(s) 2146
BsrI ACTGG 4 cut(s) 140, 1015, 1069, 1990
BssAI RCCGGY 1 cut(s) 72
BssECI CCNNGG 3 cut(s) 60, 186, 1125
BssHII GCGCGC 1 cut(s) 1430
BssMI GATC 8 cut(s) 474, 556, 1101, 1486, 1572, 1975, 2082, 2227
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1832
BssT1I CCWWGG 2 cut(s) 186, 1125
Bst2BI CACGAG 1 cut(s) 1832
Bst2UI CCWGG 7 cut(s) 61, 89, 657, 785, 1867, 2171, 2365
Bst4CI ACNGT 6 cut(s) 97, 516, 705, 964, 1233, 2323
Bst6I CTCTTC 1 cut(s) 936
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 635
BstAPI GCANNNNNTGC 1 cut(s) 2207
BstAUI TGTACA 1 cut(s) 2146
BstBI TTCGAA 2 cut(s) 81, 100
BstC8I GCNNGC 4 cut(s) 794, 798, 1302, 1432
BstEII GGTNACC 1 cut(s) 497
BstENI CCTNNNNNAGG 1 cut(s) 782
BstF5I GGATG 8 cut(s) 180, 757, 1167, 1518, 1587, 1852, 2290, 2359
BstFNI CGCG 1 cut(s) 1432
BstHHI GCGC 3 cut(s) 167, 1432, 1434
BstKTI GATC 8 cut(s) 477, 559, 1104, 1489, 1575, 1978, 2085, 2230
BstMAI GTCTC 7 cut(s) 298, 369, 522, 742, 1139, 1177, 2071
BstMBI GATC 8 cut(s) 474, 556, 1101, 1486, 1572, 1975, 2082, 2227
BstMWI GCNNNNNNNGC 9 cut(s) 62, 213, 559, 789, 802, 814, 1751, 1895, 2207
BstNI CCWGG 7 cut(s) 61, 89, 657, 785, 1867, 2171, 2365
BstNSI RCATGY 2 cut(s) 1304, 2293
BstPAI GACNNNNGTC 1 cut(s) 2300
BstPI GGTNACC 1 cut(s) 497
BstSCI CCNGG 7 cut(s) 59, 87, 655, 783, 1865, 2169, 2363
BstSFI CTRYAG 2 cut(s) 806, 2271
BstSLI GKGCMC 2 cut(s) 67, 2182
BstUI CGCG 1 cut(s) 1432
BstV1I GCAGC 6 cut(s) 792, 820, 1099, 1741, 2028, 2213
BstV2I GAAGAC 2 cut(s) 217, 474
BstX2I RGATCY 3 cut(s) 1486, 1975, 2227
BstYI RGATCY 3 cut(s) 1486, 1975, 2227
BsuRI GGCC 6 cut(s) 65, 110, 191, 1124, 1609, 2169
BtgZI GCGATG 1 cut(s) 231
BtsCI GGATG 8 cut(s) 180, 757, 1167, 1518, 1587, 1852, 2290, 2359
BtsI GCAGTG 2 cut(s) 1962, 2079
BtsIMutI CAGTG 5 cut(s) 1229, 1962, 2079, 2328, 2439
BveI ACCTGC 2 cut(s) 287, 1951
Cac8I GCNNGC 4 cut(s) 794, 798, 1302, 1432
CaiI CAGNNNCTG 1 cut(s) 1064
CciI TCATGA 2 cut(s) 1911, 2130
CfoI GCGC 3 cut(s) 167, 1432, 1434
Cfr10I RCCGGY 1 cut(s) 72
Cfr13I GGNCC 5 cut(s) 63, 64, 732, 1694, 1936
CsiI ACCWGGT 1 cut(s) 1865
Csp6I GTAC 6 cut(s) 133, 276, 399, 546, 1804, 2147
CviQI GTAC 6 cut(s) 133, 276, 399, 546, 1804, 2147
DpnI GATC 8 cut(s) 476, 558, 1103, 1488, 1574, 1977, 2084, 2229
DpnII GATC 8 cut(s) 474, 556, 1101, 1486, 1572, 1975, 2082, 2227
Eam1104I CTCTTC 1 cut(s) 936
EarI CTCTTC 1 cut(s) 936
EciI GGCGGA 1 cut(s) 220
Ecl136II GAGCTC 2 cut(s) 387, 1909
Eco130I CCWWGG 2 cut(s) 186, 1125
Eco147I AGGCCT 1 cut(s) 2169
Eco24I GRGCYC 5 cut(s) 67, 142, 153, 389, 1911
Eco31I GGTCTC 1 cut(s) 298
Eco32I GATATC 1 cut(s) 342
Eco47I GGWCC 3 cut(s) 732, 1694, 1936
Eco53kI GAGCTC 2 cut(s) 387, 1909
Eco57I CTGAAG 3 cut(s) 888, 1169, 1545
Eco88I CYCGRG 1 cut(s) 752
Eco91I GGTNACC 1 cut(s) 497
EcoICRI GAGCTC 2 cut(s) 387, 1909
EcoNI CCTNNNNNAGG 1 cut(s) 782
EcoO109I RGGNCCY 3 cut(s) 63, 64, 1694
EcoO65I GGTNACC 1 cut(s) 497
EcoRII CCWGG 7 cut(s) 59, 87, 655, 783, 1865, 2169, 2363
EcoRV GATATC 1 cut(s) 342
EcoT14I CCWWGG 2 cut(s) 186, 1125
EcoT38I GRGCYC 5 cut(s) 67, 142, 153, 389, 1911
ErhI CCWWGG 2 cut(s) 186, 1125
Esp3I CGTCTC 2 cut(s) 369, 742
FalI AAGNNNNNCTT 1 cut(s) 2418
FaqI GGGAC 3 cut(s) 627, 1802, 2281
FauI CCCGC 4 cut(s) 494, 917, 1007, 2016
FbaI TGATCA 1 cut(s) 1572
FblI GTMKAC 1 cut(s) 1627
Fnu4HI GCNGC 8 cut(s) 57, 321, 806, 809, 1088, 1755, 2017, 2202
FokI GGATG 8 cut(s) 167, 744, 1174, 1525, 1594, 1859, 2297, 2366
FriOI GRGCYC 5 cut(s) 67, 142, 153, 389, 1911
Fsp4HI GCNGC 8 cut(s) 57, 321, 806, 809, 1088, 1755, 2017, 2202
FspBI CTAG 6 cut(s) 402, 444, 873, 1497, 1794, 2235
GlaI GCGC 3 cut(s) 166, 1431, 1433
GluI GCNGC 8 cut(s) 57, 321, 806, 809, 1088, 1755, 2017, 2202
GsaI CCCAGC 2 cut(s) 1344, 1745
GsuI CTGGAG 4 cut(s) 229, 1086, 1430, 1502
HaeIII GGCC 6 cut(s) 65, 110, 191, 1124, 1609, 2169
HapII CCGG 3 cut(s) 44, 73, 332
HhaI GCGC 3 cut(s) 167, 1432, 1434
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 3 cut(s) 165, 1430, 1432
HinP1I GCGC 3 cut(s) 165, 1430, 1432
HindIII AAGCTT 1 cut(s) 648
HpaII CCGG 3 cut(s) 44, 73, 332
HphI GGTGA 4 cut(s) 283, 509, 865, 1177
Hpy166II GTNNAC 6 cut(s) 9, 460, 960, 1008, 1628, 2147
Hpy188III TCNNGA 9 cut(s) 246, 994, 1309, 1481, 1589, 1878, 1912, 2131, 2337
Hpy8I GTNNAC 6 cut(s) 9, 460, 960, 1008, 1628, 2147
Hpy99I CGWCG 1 cut(s) 364
HpyCH4III ACNGT 6 cut(s) 97, 516, 705, 964, 1233, 2323
HpyCH4IV ACGT 1 cut(s) 2277
HpyF10VI GCNNNNNNNGC 9 cut(s) 62, 213, 559, 789, 802, 814, 1751, 1895, 2207
HpySE526I ACGT 1 cut(s) 2277
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 3 cut(s) 165, 1430, 1432
KpnI GGTACC 1 cut(s) 279
Ksp22I TGATCA 1 cut(s) 1572
Kzo9I GATC 8 cut(s) 474, 556, 1101, 1486, 1572, 1975, 2082, 2227
LmnI GCTCC 7 cut(s) 125, 148, 384, 430, 676, 1353, 1906
Lsp1109I GCAGC 6 cut(s) 792, 820, 1099, 1741, 2028, 2213
LweI GCATC 6 cut(s) 208, 271, 766, 826, 874, 2302
MabI ACCWGGT 1 cut(s) 1865
MaeI CTAG 6 cut(s) 402, 444, 873, 1497, 1794, 2235
MaeII ACGT 1 cut(s) 2277
MaeIII GTNAC 4 cut(s) 91, 497, 1060, 1981
MalI GATC 8 cut(s) 476, 558, 1103, 1488, 1574, 1977, 2084, 2229
MboI GATC 8 cut(s) 474, 556, 1101, 1486, 1572, 1975, 2082, 2227
MfeI CAATTG 2 cut(s) 1491, 2119
MflI RGATCY 3 cut(s) 1486, 1975, 2227
MhlI GDGCHC 8 cut(s) 67, 142, 153, 209, 389, 829, 1911, 2182
MlyI GAGTC 6 cut(s) 488, 649, 731, 872, 1141, 2381
MmeI TCCRAC 3 cut(s) 201, 261, 721
MslI CAYNNNNRTG 1 cut(s) 1819
MspCI CTTAAG 1 cut(s) 635
MspI CCGG 3 cut(s) 44, 73, 332
MspR9I CCNGG 7 cut(s) 61, 89, 657, 785, 1867, 2171, 2365
MunI CAATTG 2 cut(s) 1491, 2119
Mva1269I GAATGC 1 cut(s) 1084
MvaI CCWGG 7 cut(s) 61, 89, 657, 785, 1867, 2171, 2365
MvnI CGCG 1 cut(s) 1432
MwoI GCNNNNNNNGC 9 cut(s) 62, 213, 559, 789, 802, 814, 1751, 1895, 2207
NdeII GATC 8 cut(s) 474, 556, 1101, 1486, 1572, 1975, 2082, 2227
NlaIV GGNNCC 8 cut(s) 65, 150, 277, 336, 661, 733, 1488, 1938
NmuCI GTSAC 1 cut(s) 497
NspI RCATGY 2 cut(s) 1304, 2293
NspV TTCGAA 2 cut(s) 81, 100
PaeI GCATGC 1 cut(s) 1304
PaeR7I CTCGAG 1 cut(s) 752
PagI TCATGA 2 cut(s) 1911, 2130
PauI GCGCGC 1 cut(s) 1430
PceI AGGCCT 1 cut(s) 2169
PctI GAATGC 1 cut(s) 1084
PfeI GAWTC 8 cut(s) 78, 686, 929, 1055, 1516, 1722, 2140, 2412
PflMI CCANNNNNTGG 3 cut(s) 1000, 1162, 1616
PkrI GCNGC 8 cut(s) 58, 322, 807, 810, 1089, 1756, 2018, 2203
PleI GAGTC 6 cut(s) 487, 648, 730, 871, 1141, 2380
PpsI GAGTC 6 cut(s) 487, 648, 730, 871, 1141, 2380
PpuMI RGGWCCY 1 cut(s) 1694
PshAI GACNNNNGTC 1 cut(s) 2300
PshBI ATTAAT 1 cut(s) 1613
PsiI TTATAA 1 cut(s) 1467
Psp124BI GAGCTC 2 cut(s) 389, 1911
Psp5II RGGWCCY 1 cut(s) 1694
Psp6I CCWGG 7 cut(s) 59, 87, 655, 783, 1865, 2169, 2363
PspEI GGTNACC 1 cut(s) 497
PspFI CCCAGC 2 cut(s) 1340, 1741
PspGI CCWGG 7 cut(s) 59, 87, 655, 783, 1865, 2169, 2363
PspN4I GGNNCC 8 cut(s) 65, 150, 277, 336, 661, 733, 1488, 1938
PspOMI GGGCCC 1 cut(s) 63
PspPI GGNCC 5 cut(s) 63, 64, 732, 1694, 1936
PspPPI RGGWCCY 1 cut(s) 1694
PspXI VCTCGAGB 1 cut(s) 752
PsrI GAACNNNNNNTAC 2 cut(s) 318, 350
PstI CTGCAG 2 cut(s) 810, 2275
PstNI CAGNNNCTG 1 cut(s) 1064
PsuI RGATCY 3 cut(s) 1486, 1975, 2227
PteI GCGCGC 1 cut(s) 1430
RsaI GTAC 6 cut(s) 134, 277, 400, 547, 1805, 2148
RsaNI GTAC 6 cut(s) 133, 276, 399, 546, 1804, 2147
RseI CAYNNNNRTG 1 cut(s) 1819
SacI GAGCTC 2 cut(s) 389, 1911
SatI GCNGC 8 cut(s) 57, 321, 806, 809, 1088, 1755, 2017, 2202
Sau3AI GATC 8 cut(s) 474, 556, 1101, 1486, 1572, 1975, 2082, 2227
Sau96I GGNCC 5 cut(s) 63, 64, 732, 1694, 1936
SchI GAGTC 6 cut(s) 488, 649, 731, 872, 1141, 2381
ScrFI CCNGG 7 cut(s) 61, 89, 657, 785, 1867, 2171, 2365
SduI GDGCHC 8 cut(s) 67, 142, 153, 209, 389, 829, 1911, 2182
SexAI ACCWGGT 1 cut(s) 1865
SfaNI GCATC 6 cut(s) 208, 271, 766, 826, 874, 2302
SfcI CTRYAG 2 cut(s) 806, 2271
Sfr274I CTCGAG 1 cut(s) 752
SfuI TTCGAA 2 cut(s) 81, 100
SinI GGWCC 3 cut(s) 732, 1694, 1936
SlaI CTCGAG 1 cut(s) 752
SmiMI CAYNNNNRTG 1 cut(s) 1819
SmlI CTYRAG 8 cut(s) 200, 420, 635, 752, 1331, 2158, 2216, 2369
SmoI CTYRAG 8 cut(s) 200, 420, 635, 752, 1331, 2158, 2216, 2369
SpeI ACTAGT 2 cut(s) 401, 1793
SphI GCATGC 1 cut(s) 1304
SseBI AGGCCT 1 cut(s) 2169
SspMI CTAG 6 cut(s) 402, 444, 873, 1497, 1794, 2235
SstI GAGCTC 2 cut(s) 389, 1911
StuI AGGCCT 1 cut(s) 2169
StyD4I CCNGG 7 cut(s) 59, 87, 655, 783, 1865, 2169, 2363
StyI CCWWGG 2 cut(s) 186, 1125
TaaI ACNGT 6 cut(s) 97, 516, 705, 964, 1233, 2323
TaiI ACGT 1 cut(s) 2280
TaqI TCGA 7 cut(s) 81, 100, 477, 746, 753, 829, 1308
TatI WGTACW 1 cut(s) 2146
TauI GCSGC 2 cut(s) 59, 323
TfiI GAWTC 8 cut(s) 78, 686, 929, 1055, 1516, 1722, 2140, 2412
TscAI CASTG 5 cut(s) 1236, 1962, 2079, 2328, 2446
TseFI GTSAC 1 cut(s) 497
TseI GCWGC 6 cut(s) 805, 808, 1087, 1754, 2016, 2201
Tsp45I GTSAC 1 cut(s) 497
TspDTI ATGAA 6 cut(s) 303, 1183, 1554, 1928, 2009, 2119
TspGWI ACGGA 2 cut(s) 348, 724
TspRI CASTG 5 cut(s) 1236, 1962, 2079, 2328, 2446
Van91I CCANNNNNTGG 3 cut(s) 1000, 1162, 1616
Vha464I CTTAAG 1 cut(s) 635
VpaK11BI GGWCC 3 cut(s) 732, 1694, 1936
VspI ATTAAT 1 cut(s) 1613
XagI CCTNNNNNAGG 1 cut(s) 782
XapI RAATTY 2 cut(s) 286, 1270
XceI RCATGY 2 cut(s) 1304, 2293
XhoI CTCGAG 1 cut(s) 752
XmiI GTMKAC 1 cut(s) 1627
XspI CTAG 6 cut(s) 402, 444, 873, 1497, 1794, 2235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.