MD04G1078600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001384
Physical Location & Seq
Forward (+)
10741122 .. 10745665
4544 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1078600.v1.1.491

Sequence Viewer

Length: 2310 bp
ATGGCGACTACCAACACCACCACGCAGGCCGTGGGAGTTGAGGGGGTGGACGCGTCAGCATCGGCGGACGAGGCGGCGGCTAAAGCCGTGCACAAGAGGTACGAAGGGCTTGTGATGGTACGGACGAAGGCGATAAAGGGGAAAGGGGCTTGGTACTGGGCTCACTTGGAGCCTATGTTGGTCCACAACACCGACACTGGTCTGCCCAAAGCGGTGAAGCTCCGGTGCTCCTTTTGCGACGCCTTGTTCTCCGCCTCGAACCCGTCTCGCACCGCCTCCGAGCACTTAAAGCGCGGGACTTGCCCCAATTTCAACTCCGTCGCGAAACCCATTTCGTCCCTCTCGCCGTCCTCCACCATCAACTTGCCTCCGTCGCCTGGCCCCGTCCACCATACCAGCCGCAAGCGGAGCTCGTCTTCCGTTTCCGTTTCGGCTTCAACGTCGTACCATGTTCCGCCGCTCGCGATAGTGGACCCGACCCGGTTCTGCGGCGAGCTCACTTACTCTCCGGTGACCGCGACGGCGCATACGGCGGTGACCCACCAGCCGCATTTGGTGTTGTCGGGTGGGAAAGAGGACTTGGAAGCTCTGGCGATGCTGGAGGACAGCGTAAAGAAGCTCAAGAGCCCCAAAACTTCACCCGGTCCGACGCTGAGTAAGACGCAGGTGGACGTTGCGCTTGATTTTCTGGCCGATTGGGTGTTTGAGTCTTGTGGGTCGGTGTCGTTTTCGAGCCTCGAGCACCCAAAGTTCAGAGCTTTTCTCAACCAGGTGGGTCTGCGGGCGATTTCCGGGCGGGAGTTCACTGGGTCGAGACTCGACGCCAAGTTTGAGGAAGCGAAGGCCGAGTCCGAGGCCCGAATTCACGACGCCATGTTCTTTCAGATTGCTTCTGATGGGTGGAAGAGCAAGACTTTTGGCGCGTTTGGGGAAGATGGTCTTGTAAATTTGACCGTGAATCTTCCCAATGGGACTAGTGTGTACAGGAAGGCGGTGTTTGTCGGCGGCTCTGTACCTTCGAAGTATGCCGAGGATGTTTTGTGGGAGACAGTGACAAGCATTTGTGGAAATGTTGTGCAGCAGTGTGTAGGAATAGTAGCAGACAAGTTTAAGTCCAAGGCACTAAGGAATTTAGAGAATCAGAATCACTGGATGGTTAATCTTTCTTGTCAGTTTCAGGGTTTCAATAGTTTGATTAAGGATTTCAGTAAGGAACTTCCCTTGTTTAAGGATGTCGCCGAGAGTTGTTTTAAGATTGCGAATTTCGTGAATAACAAGTCTCAGGTTAGGAGTAGCTTTCATAAGTATCAGTCCCAGGAGTATGGTCATGCCGGGCTGTTAAGAGTGCCGTTGCGTGAGTTCGAAATGGTTAACTTTGGAGCAGTGCATATATTGTTCGAGGATATTCTGAGCTCAGCCGGGGCACTTCAGTTGGTCCTGCTAGACGAATCTTATAAAGTTGCCTCCATGGAGGATCCAATGGCTAGAGAAGTCGCCGAGATGATTGGGGATGTGGGGTTTTGGAATGAATTGCAGGCGGTGCATTCGTTGGTTAAGTTGATCAAGGACATGGCTCAAGAGATTGAGACGGAGAGGCCGCTGGTTGGTAAATGCCTCCCACTTTGGGATGAACTAAGAACAAAAGTGAAGGATTGGTGTGCCAGTTTCCACATTCCAGAAGAACCGGTGGAGAAGGTGATTGAAAGGCGGTTCAGGAAGAATTATCATCCCGCTTGGGCGGCTGCGTTTATACTTGATCCGCTCTATTTAATTAGGGACACTAGTGGGAAGTATCTTCCACCATTCAAGTTGTTGACGCCAGAGCAGGAGAAGGATGTGGACAAGCTTATAACCCGGCTTGTGTCGAGGGAGGAAGCCCATATAGCACTAATGGAACTCATGAAATGGAGAACGGAGGGGCTTGATCAGGTATATGCGCGCGCTGTACAGATGAAGGAGAGGGACCCCAATACAGGGAAGATGAGAATTGCCAACCCACAGAGCAGTAGGCTTGTATGGGAAACACACCTCACCGAGTTTAAGTCACTAGGGAAAGTTGCAGTTAGGCTCATCTTCCTTCATGCAACGTCGTGTGGGTTCAAATGTAATTGGTCTCTATTGAGATGGGTGTCTGCCCATGGCCACTCAAGGGTGGGAATTGACAAGGCACAGAAGTTGATATTCATTGCAGCTCATTCCAAGCTCGAAAGGCGGGATTATTCCTTCGAAGAAGACAAGGATGCGGAGCTACTCGCCTTAGCAAACGAAGCATTCGACAAAGATATCGTTGGACTGGAGAGGCCACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF659

Protein Analysis

770

Amino Acids

85.56

Weight (kDa)

7.88

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7963 PF25908 21 - 105 4.7e-47 Domain of unknown function (DUF7963)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1455, 1850
AarI CACCTGC 1 cut(s) 655
Acc36I ACCTGC 1 cut(s) 655
AccBSI CCGCTC 2 cut(s) 460, 1762
AccII CGCG 8 cut(s) 53, 294, 323, 464, 518, 923, 1939, 1941
AclWI GGATC 3 cut(s) 1469, 1482, 1751
AcoI YGGCCR 2 cut(s) 690, 2140
AcsI RAATTY 4 cut(s) 861, 946, 1129, 1261
AcuI CTGAAG 1 cut(s) 1412
AcyI GRCGYC 4 cut(s) 240, 822, 870, 1817
AfaI GTAC 7 cut(s) 101, 120, 155, 446, 983, 1014, 1947
AfiI CCNNNNNNNGG 6 cut(s) 377, 1604, 1624, 1973, 1974, 2149
AflIII ACRYGT 1 cut(s) 51
AgeI ACCGGT 1 cut(s) 1684
AgsI TTSAA 6 cut(s) 313, 438, 1186, 1703, 1807, 2101
AhlI ACTAGT 2 cut(s) 974, 1781
AjnI CCWGG 3 cut(s) 376, 768, 1314
AjuI GAANNNNNNNTTGG 2 cut(s) 563, 595
Alw21I GWGCWC 7 cut(s) 93, 230, 285, 413, 498, 744, 1415
Alw26I GTCTC 6 cut(s) 270, 808, 1040, 1284, 1580, 2118
Alw44I GTGCAC 1 cut(s) 89
AlwI GGATC 3 cut(s) 1469, 1482, 1751
Ama87I CYCGRG 1 cut(s) 737
AoxI GGCC 8 cut(s) 27, 379, 690, 843, 855, 1595, 2140, 2300
ApaLI GTGCAC 1 cut(s) 89
ApeKI GCWGC 3 cut(s) 1078, 1742, 2189
ApoI RAATTY 4 cut(s) 861, 946, 1129, 1261
ArsI GACNNNNNNTTYG 4 cut(s) 812, 833, 844, 865
AsiGI ACCGGT 1 cut(s) 1684
AspLEI GCGC 7 cut(s) 294, 526, 679, 923, 1939, 1941, 1943
AspS9I GGNCC 7 cut(s) 181, 380, 472, 644, 856, 1435, 1963
AsuC2I CCSGG 6 cut(s) 481, 642, 793, 1333, 1420, 1855
AsuHPI GGTGA 6 cut(s) 226, 523, 547, 630, 1708, 2023
AsuII TTCGAA 3 cut(s) 1019, 1362, 2226
AvaI CYCGRG 1 cut(s) 737
AvaII GGWCC 5 cut(s) 181, 472, 644, 1435, 1963
BaeGI GKGCMC 2 cut(s) 93, 1426
BalI TGGCCA 1 cut(s) 2142
BamHI GGATCC 1 cut(s) 1474
BanII GRGCYC 5 cut(s) 163, 413, 498, 629, 1415
BbsI GAAGAC 2 cut(s) 408, 2238
Bbv12I GWGCWC 7 cut(s) 93, 230, 285, 413, 498, 744, 1415
BbvI GCAGC 3 cut(s) 1090, 1729, 2201
BccI CCATC 6 cut(s) 109, 365, 890, 929, 1147, 2118
BceAI ACGGC 6 cut(s) 14, 71, 331, 537, 546, 1333
BciT130I CCWGG 3 cut(s) 378, 770, 1316
BclI TGATCA 2 cut(s) 1560, 1924
BcnI CCSGG 6 cut(s) 481, 642, 793, 1333, 1420, 1855
BcoDI GTCTC 6 cut(s) 270, 808, 1040, 1284, 1580, 2118
BcuI ACTAGT 2 cut(s) 974, 1781
BfaI CTAG 5 cut(s) 975, 1442, 1485, 1782, 2048
BfuAI ACCTGC 1 cut(s) 655
BlpI GCTNAGC 1 cut(s) 1414
Bme1390I CCNGG 9 cut(s) 378, 481, 642, 770, 793, 1316, 1333, 1420, 1855
Bme18I GGWCC 5 cut(s) 181, 472, 644, 1435, 1963
BmeT110I CYCGRG 1 cut(s) 737
BmgT120I GGNCC 7 cut(s) 181, 380, 472, 644, 856, 1435, 1963
BmiI GGNNCC 6 cut(s) 171, 382, 474, 1476, 1964, 1965
BmrFI CCNGG 9 cut(s) 378, 481, 642, 770, 793, 1316, 1333, 1420, 1855
BmrI ACTGGG 2 cut(s) 166, 816
BmsI GCATC 3 cut(s) 68, 585, 2230
BmuI ACTGGG 2 cut(s) 166, 816
BoxI GACNNNNGTC 1 cut(s) 198
BpiI GAAGAC 2 cut(s) 408, 2238
BplI GAGNNNNNCTC 2 cut(s) 747, 779
BpmI CTGGAG 1 cut(s) 620
Bpu10I CCTNAGC 1 cut(s) 2257
Bpu1102I GCTNAGC 1 cut(s) 1414
Bpu14I TTCGAA 3 cut(s) 1019, 1362, 2226
BpuEI CTTGAG 3 cut(s) 605, 1560, 2131
BpuMI CCSGG 6 cut(s) 481, 642, 793, 1333, 1420, 1855
BsaHI GRCGYC 4 cut(s) 240, 822, 870, 1817
BsaI GGTCTC 1 cut(s) 2118
BsaJI CCNNGG 8 cut(s) 30, 852, 1029, 1116, 1314, 1419, 1467, 2137
BsaWI WCCGGW 3 cut(s) 222, 508, 1684
BsaXI ACNNNNNCTCC 4 cut(s) 161, 191, 490, 520
Bsc4I CCNNNNNNNGG 6 cut(s) 377, 1604, 1624, 1973, 1974, 2149
Bse118I RCCGGY 1 cut(s) 1684
Bse1I ACTGG 6 cut(s) 161, 202, 811, 1154, 1662, 2298
Bse3DI GCAATG 1 cut(s) 2184
BseBI CCWGG 3 cut(s) 378, 770, 1316
BseDI CCNNGG 8 cut(s) 30, 852, 1029, 1116, 1314, 1419, 1467, 2137
BseGI GGATG 8 cut(s) 1039, 1158, 1237, 1516, 1633, 1726, 1840, 2245
BseLI CCNNNNNNNGG 6 cut(s) 377, 1604, 1624, 1973, 1974, 2149
BseMI GCAATG 1 cut(s) 2184
BseMII CTCAG 4 cut(s) 644, 1295, 1400, 1428
BseNI ACTGG 6 cut(s) 161, 202, 811, 1154, 1662, 2298
BsePI GCGCGC 2 cut(s) 1937, 1939
BseSI GKGCMC 2 cut(s) 93, 1426
BseXI GCAGC 3 cut(s) 1090, 1729, 2201
BsgI GTGCAG 1 cut(s) 1097
Bsh1236I CGCG 8 cut(s) 53, 294, 323, 464, 518, 923, 1939, 1941
BshFI GGCC 8 cut(s) 29, 381, 692, 845, 857, 1597, 2142, 2302
BshTI ACCGGT 1 cut(s) 1684
BsiHKAI GWGCWC 7 cut(s) 93, 230, 285, 413, 498, 744, 1415
BsiHKCI CYCGRG 1 cut(s) 737
BsiSI CCGG 9 cut(s) 223, 481, 509, 642, 792, 1332, 1419, 1685, 1855
BslFI GGGAC 6 cut(s) 310, 322, 985, 1297, 1790, 1976
BslI CCNNNNNNNGG 6 cut(s) 377, 1604, 1624, 1973, 1974, 2149
BsmAI GTCTC 6 cut(s) 270, 808, 1040, 1284, 1580, 2118
BsmBI CGTCTC 2 cut(s) 270, 1580
BsmFI GGGAC 6 cut(s) 310, 322, 985, 1297, 1790, 1976
BsmI GAATGC 2 cut(s) 1543, 2270
BsnI GGCC 8 cut(s) 29, 381, 692, 845, 857, 1597, 2142, 2302
Bso31I GGTCTC 1 cut(s) 2118
BsoBI CYCGRG 1 cut(s) 737
Bsp119I TTCGAA 3 cut(s) 1019, 1362, 2226
Bsp1407I TGTACA 2 cut(s) 981, 1945
Bsp143I GATC 4 cut(s) 1474, 1560, 1756, 1924
Bsp1720I GCTNAGC 1 cut(s) 1414
Bsp19I CCATGG 2 cut(s) 1467, 2137
Bsp68I TCGCGA 2 cut(s) 323, 464
BspANI GGCC 8 cut(s) 29, 381, 692, 845, 857, 1597, 2142, 2302
BspCNI CTCAG 4 cut(s) 645, 1294, 1401, 1427
BspFNI CGCG 8 cut(s) 53, 294, 323, 464, 518, 923, 1939, 1941
BspHI TCATGA 1 cut(s) 1899
BspLI GGNNCC 6 cut(s) 171, 382, 474, 1476, 1964, 1965
BspMI ACCTGC 1 cut(s) 655
BspPI GGATC 3 cut(s) 1469, 1482, 1751
BspQI GCTCTTC 1 cut(s) 899
BspT104I TTCGAA 3 cut(s) 1019, 1362, 2226
BspTNI GGTCTC 1 cut(s) 2118
BsrBI CCGCTC 2 cut(s) 460, 1762
BsrDI GCAATG 1 cut(s) 2184
BsrFI RCCGGY 1 cut(s) 1684
BsrGI TGTACA 2 cut(s) 981, 1945
BsrI ACTGG 6 cut(s) 161, 202, 811, 1154, 1662, 2298
BssAI RCCGGY 1 cut(s) 1684
BssECI CCNNGG 8 cut(s) 30, 852, 1029, 1116, 1314, 1419, 1467, 2137
BssHII GCGCGC 2 cut(s) 1937, 1939
BssMI GATC 4 cut(s) 1474, 1560, 1756, 1924
BssNI GRCGYC 4 cut(s) 240, 822, 870, 1817
BssT1I CCWWGG 3 cut(s) 1116, 1467, 2137
Bst2UI CCWGG 3 cut(s) 378, 770, 1316
Bst4CI ACNGT 2 cut(s) 955, 1051
Bst6I CTCTTC 1 cut(s) 899
BstACI GRCGYC 4 cut(s) 240, 822, 870, 1817
BstAPI GCANNNNNTGC 1 cut(s) 1540
BstAUI TGTACA 2 cut(s) 981, 1945
BstBI TTCGAA 3 cut(s) 1019, 1362, 2226
BstC8I GCNNGC 8 cut(s) 27, 404, 462, 494, 783, 1536, 1939, 1941
BstDEI CTNAG 7 cut(s) 653, 1124, 1281, 1409, 1414, 1634, 2257
BstDSI CCRYGG 3 cut(s) 30, 1467, 2137
BstEII GGTNACC 2 cut(s) 511, 535
BstF5I GGATG 8 cut(s) 1039, 1158, 1237, 1516, 1633, 1726, 1840, 2245
BstFNI CGCG 8 cut(s) 53, 294, 323, 464, 518, 923, 1939, 1941
BstHHI GCGC 7 cut(s) 294, 526, 679, 923, 1939, 1941, 1943
BstKTI GATC 4 cut(s) 1477, 1563, 1759, 1927
BstMAI GTCTC 6 cut(s) 270, 808, 1040, 1284, 1580, 2118
BstMBI GATC 4 cut(s) 1474, 1560, 1756, 1924
BstNI CCWGG 3 cut(s) 378, 770, 1316
BstPAI GACNNNNGTC 1 cut(s) 198
BstPI GGTNACC 2 cut(s) 511, 535
BstSCI CCNGG 9 cut(s) 376, 479, 640, 768, 791, 1314, 1331, 1418, 1853
BstSLI GKGCMC 2 cut(s) 93, 1426
BstUI CGCG 8 cut(s) 53, 294, 323, 464, 518, 923, 1939, 1941
BstV1I GCAGC 3 cut(s) 1090, 1729, 2201
BstV2I GAAGAC 2 cut(s) 408, 2238
BstX2I RGATCY 1 cut(s) 1474
BstXI CCANNNNNNTGG 1 cut(s) 1322
BstYI RGATCY 1 cut(s) 1474
BsuRI GGCC 8 cut(s) 29, 381, 692, 845, 857, 1597, 2142, 2302
BtgI CCRYGG 3 cut(s) 30, 1467, 2137
BtgZI GCGATG 1 cut(s) 608
BtsCI GGATG 8 cut(s) 1039, 1158, 1237, 1516, 1633, 1726, 1840, 2245
BtsI GCAGTG 2 cut(s) 1088, 1389
BtsIMutI CAGTG 6 cut(s) 195, 804, 1056, 1088, 1147, 1389
BtuMI TCGCGA 2 cut(s) 323, 464
BveI ACCTGC 1 cut(s) 655
Cac8I GCNNGC 8 cut(s) 27, 404, 462, 494, 783, 1536, 1939, 1941
CciI TCATGA 1 cut(s) 1899
CfoI GCGC 7 cut(s) 294, 526, 679, 923, 1939, 1941, 1943
Cfr10I RCCGGY 1 cut(s) 1684
Cfr13I GGNCC 7 cut(s) 181, 380, 472, 644, 856, 1435, 1963
CpoI CGGWCCG 1 cut(s) 644
CseI GACGC 8 cut(s) 42, 59, 248, 658, 670, 830, 878, 1825
CsiI ACCWGGT 1 cut(s) 768
Csp6I GTAC 7 cut(s) 100, 119, 154, 445, 982, 1013, 1946
CspAI ACCGGT 1 cut(s) 1684
CspI CGGWCCG 1 cut(s) 644
CviAII CATG 8 cut(s) 449, 874, 1328, 1468, 1570, 1900, 2081, 2138
CviQI GTAC 7 cut(s) 100, 119, 154, 445, 982, 1013, 1946
DdeI CTNAG 7 cut(s) 653, 1124, 1281, 1409, 1414, 1634, 2257
DpnI GATC 4 cut(s) 1476, 1562, 1758, 1926
DpnII GATC 4 cut(s) 1474, 1560, 1756, 1924
EaeI YGGCCR 2 cut(s) 690, 2140
Eam1104I CTCTTC 1 cut(s) 899
EarI CTCTTC 1 cut(s) 899
EciI GGCGGA 3 cut(s) 80, 241, 444
Ecl136II GAGCTC 3 cut(s) 411, 496, 1413
Eco130I CCWWGG 3 cut(s) 1116, 1467, 2137
Eco24I GRGCYC 5 cut(s) 163, 413, 498, 629, 1415
Eco31I GGTCTC 1 cut(s) 2118
Eco32I GATATC 1 cut(s) 2284
Eco47I GGWCC 5 cut(s) 181, 472, 644, 1435, 1963
Eco53kI GAGCTC 3 cut(s) 411, 496, 1413
Eco57I CTGAAG 1 cut(s) 1412
Eco88I CYCGRG 1 cut(s) 737
Eco91I GGTNACC 2 cut(s) 511, 535
EcoICRI GAGCTC 3 cut(s) 411, 496, 1413
EcoO109I RGGNCCY 1 cut(s) 1963
EcoO65I GGTNACC 2 cut(s) 511, 535
EcoRI GAATTC 1 cut(s) 861
EcoRII CCWGG 3 cut(s) 376, 768, 1314
EcoRV GATATC 1 cut(s) 2284
EcoT14I CCWWGG 3 cut(s) 1116, 1467, 2137
EcoT38I GRGCYC 5 cut(s) 163, 413, 498, 629, 1415
ErhI CCWWGG 3 cut(s) 1116, 1467, 2137
Esp3I CGTCTC 2 cut(s) 270, 1580
FaeI CATG 8 cut(s) 452, 877, 1331, 1471, 1573, 1903, 2084, 2141
FalI AAGNNNNNCTT 2 cut(s) 924, 956
FaqI GGGAC 6 cut(s) 310, 322, 985, 1297, 1790, 1976
FatI CATG 8 cut(s) 448, 873, 1327, 1467, 1569, 1899, 2080, 2137
FauI CCCGC 5 cut(s) 287, 774, 789, 1738, 2205
FbaI TGATCA 2 cut(s) 1560, 1924
FokI GGATG 8 cut(s) 1046, 1165, 1244, 1523, 1640, 1713, 1847, 2252
FriOI GRGCYC 5 cut(s) 163, 413, 498, 629, 1415
FspBI CTAG 5 cut(s) 975, 1442, 1485, 1782, 2048
GlaI GCGC 7 cut(s) 293, 525, 678, 922, 1938, 1940, 1942
GsuI CTGGAG 1 cut(s) 620
HaeIII GGCC 8 cut(s) 29, 381, 692, 845, 857, 1597, 2142, 2302
HapII CCGG 9 cut(s) 223, 481, 509, 642, 792, 1332, 1419, 1685, 1855
HgaI GACGC 8 cut(s) 42, 59, 248, 658, 670, 830, 878, 1825
HhaI GCGC 7 cut(s) 294, 526, 679, 923, 1939, 1941, 1943
Hin1I GRCGYC 4 cut(s) 240, 822, 870, 1817
Hin1II CATG 8 cut(s) 452, 877, 1331, 1471, 1573, 1903, 2084, 2141
Hin6I GCGC 7 cut(s) 292, 524, 677, 921, 1937, 1939, 1941
HinP1I GCGC 7 cut(s) 292, 524, 677, 921, 1937, 1939, 1941
HincII GTYRAC 2 cut(s) 1372, 1815
HindII GTYRAC 2 cut(s) 1372, 1815
HindIII AAGCTT 1 cut(s) 1844
HinfI GANTC 7 cut(s) 707, 816, 848, 958, 1138, 1144, 1448
HpaI GTTAAC 1 cut(s) 1372
HpaII CCGG 9 cut(s) 223, 481, 509, 642, 792, 1332, 1419, 1685, 1855
HphI GGTGA 6 cut(s) 226, 523, 547, 630, 1708, 2023
Hpy188I TCNGA 8 cut(s) 280, 648, 755, 853, 885, 895, 1143, 1410
Hpy99I CGWCG 9 cut(s) 242, 323, 376, 445, 523, 652, 824, 872, 2092
HpyCH4III ACNGT 2 cut(s) 955, 1051
HpyCH4IV ACGT 3 cut(s) 440, 672, 2087
HpyCH4V TGCA 8 cut(s) 91, 1078, 1387, 1534, 1543, 2060, 2084, 2189
HpyF3I CTNAG 7 cut(s) 653, 1124, 1281, 1409, 1414, 1634, 2257
HpySE526I ACGT 3 cut(s) 440, 672, 2087
Hsp92I GRCGYC 4 cut(s) 240, 822, 870, 1817
Hsp92II CATG 8 cut(s) 452, 877, 1331, 1471, 1573, 1903, 2084, 2141
HspAI GCGC 7 cut(s) 292, 524, 677, 921, 1937, 1939, 1941
KflI GGGWCCC 1 cut(s) 1963
Ksp22I TGATCA 2 cut(s) 1560, 1924
KspAI GTTAAC 1 cut(s) 1372
Kzo9I GATC 4 cut(s) 1474, 1560, 1756, 1924
LguI GCTCTTC 1 cut(s) 899
LmnI GCTCC 6 cut(s) 169, 225, 233, 408, 1379, 2245
Lsp1109I GCAGC 3 cut(s) 1090, 1729, 2201
LweI GCATC 3 cut(s) 68, 585, 2230
MabI ACCWGGT 1 cut(s) 768
MaeI CTAG 5 cut(s) 975, 1442, 1485, 1782, 2048
MaeII ACGT 3 cut(s) 440, 672, 2087
MaeIII GTNAC 4 cut(s) 511, 535, 1051, 2043
MalI GATC 4 cut(s) 1476, 1562, 1758, 1926
MbiI CCGCTC 2 cut(s) 460, 1762
MboI GATC 4 cut(s) 1474, 1560, 1756, 1924
MflI RGATCY 1 cut(s) 1474
MlsI TGGCCA 1 cut(s) 2142
MluI ACGCGT 1 cut(s) 51
MluNI TGGCCA 1 cut(s) 2142
MlyI GAGTC 3 cut(s) 716, 810, 857
MmeI TCCRAC 2 cut(s) 671, 2269
Mox20I TGGCCA 1 cut(s) 2142
MscI TGGCCA 1 cut(s) 2142
Msp20I TGGCCA 1 cut(s) 2142
MspA1I CMGCKG 1 cut(s) 1600
MspI CCGG 9 cut(s) 223, 481, 509, 642, 792, 1332, 1419, 1685, 1855
MspR9I CCNGG 9 cut(s) 378, 481, 642, 770, 793, 1316, 1333, 1420, 1855
Mva1269I GAATGC 2 cut(s) 1543, 2270
MvaI CCWGG 3 cut(s) 378, 770, 1316
MvnI CGCG 8 cut(s) 53, 294, 323, 464, 518, 923, 1939, 1941
NciI CCSGG 6 cut(s) 481, 642, 793, 1333, 1420, 1855
NcoI CCATGG 2 cut(s) 1467, 2137
NdeII GATC 4 cut(s) 1474, 1560, 1756, 1924
NlaIII CATG 8 cut(s) 452, 877, 1331, 1471, 1573, 1903, 2084, 2141
NlaIV GGNNCC 6 cut(s) 171, 382, 474, 1476, 1964, 1965
NmeAIII GCCGAG 4 cut(s) 871, 1054, 1264, 1522
NmuCI GTSAC 4 cut(s) 511, 535, 1051, 2043
NruI TCGCGA 2 cut(s) 323, 464
NspV TTCGAA 3 cut(s) 1019, 1362, 2226
PaeR7I CTCGAG 1 cut(s) 737
PagI TCATGA 1 cut(s) 1899
PaqCI CACCTGC 1 cut(s) 655
PauI GCGCGC 2 cut(s) 1937, 1939
PciSI GCTCTTC 1 cut(s) 899
PcsI WCGNNNNNNNCGW 2 cut(s) 437, 2271
PctI GAATGC 2 cut(s) 1543, 2270
PfeI GAWTC 4 cut(s) 958, 1138, 1144, 1448
PinAI ACCGGT 1 cut(s) 1684
PleI GAGTC 3 cut(s) 715, 810, 856
PpsI GAGTC 3 cut(s) 715, 810, 856
PpuMI RGGWCCY 1 cut(s) 1963
PshAI GACNNNNGTC 1 cut(s) 198
PsiI TTATAA 2 cut(s) 1455, 1850
Psp124BI GAGCTC 3 cut(s) 413, 498, 1415
Psp5II RGGWCCY 1 cut(s) 1963
Psp6I CCWGG 3 cut(s) 376, 768, 1314
PspEI GGTNACC 2 cut(s) 511, 535
PspGI CCWGG 3 cut(s) 376, 768, 1314
PspN4I GGNNCC 6 cut(s) 171, 382, 474, 1476, 1964, 1965
PspPI GGNCC 7 cut(s) 181, 380, 472, 644, 856, 1435, 1963
PspPPI RGGWCCY 1 cut(s) 1963
PspXI VCTCGAGB 1 cut(s) 737
PsuI RGATCY 1 cut(s) 1474
PteI GCGCGC 2 cut(s) 1937, 1939
RruI TCGCGA 2 cut(s) 323, 464
RsaI GTAC 7 cut(s) 101, 120, 155, 446, 983, 1014, 1947
RsaNI GTAC 7 cut(s) 100, 119, 154, 445, 982, 1013, 1946
Rsr2I CGGWCCG 1 cut(s) 644
RsrII CGGWCCG 1 cut(s) 644
SacI GAGCTC 3 cut(s) 413, 498, 1415
SapI GCTCTTC 1 cut(s) 899
Sau3AI GATC 4 cut(s) 1474, 1560, 1756, 1924
Sau96I GGNCC 7 cut(s) 181, 380, 472, 644, 856, 1435, 1963
SchI GAGTC 3 cut(s) 716, 810, 857
ScrFI CCNGG 9 cut(s) 378, 481, 642, 770, 793, 1316, 1333, 1420, 1855
SexAI ACCWGGT 1 cut(s) 768
SfaNI GCATC 3 cut(s) 68, 585, 2230
Sfr274I CTCGAG 1 cut(s) 737
SfuI TTCGAA 3 cut(s) 1019, 1362, 2226
SinI GGWCC 5 cut(s) 181, 472, 644, 1435, 1963
SlaI CTCGAG 1 cut(s) 737
SmlI CTYRAG 4 cut(s) 620, 737, 1575, 2146
SmoI CTYRAG 4 cut(s) 620, 737, 1575, 2146
SpeI ACTAGT 2 cut(s) 974, 1781
SspMI CTAG 5 cut(s) 975, 1442, 1485, 1782, 2048
SstI GAGCTC 3 cut(s) 413, 498, 1415
StyD4I CCNGG 9 cut(s) 376, 479, 640, 768, 791, 1314, 1331, 1418, 1853
StyI CCWWGG 3 cut(s) 1116, 1467, 2137
TaaI ACNGT 2 cut(s) 955, 1051
TaiI ACGT 3 cut(s) 443, 675, 2090
TatI WGTACW 2 cut(s) 981, 1945
TauI GCSGC 9 cut(s) 77, 80, 402, 460, 492, 550, 1008, 1600, 1742
TfiI GAWTC 4 cut(s) 958, 1138, 1144, 1448
TscAI CASTG 6 cut(s) 202, 811, 1056, 1088, 1154, 1389
TseFI GTSAC 4 cut(s) 511, 535, 1051, 2043
TseI GCWGC 3 cut(s) 1078, 1742, 2189
Tsp45I GTSAC 4 cut(s) 511, 535, 1051, 2043
TspDTI ATGAA 7 cut(s) 1289, 1542, 1644, 1916, 1967, 2069, 2173
TspGWI ACGGA 7 cut(s) 136, 307, 360, 409, 415, 1604, 1928
TspRI CASTG 6 cut(s) 202, 811, 1056, 1088, 1154, 1389
VneI GTGCAC 1 cut(s) 89
VpaK11BI GGWCC 5 cut(s) 181, 472, 644, 1435, 1963
XapI RAATTY 4 cut(s) 861, 946, 1129, 1261
XcmI CCANNNNNNNNNTGG 1 cut(s) 28
XhoI CTCGAG 1 cut(s) 737
XspI CTAG 5 cut(s) 975, 1442, 1485, 1782, 2048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.